Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailDevelopment of a practical test of insulin resistance in obese Beagle dogs and effects of sc FOS
Daumas, Caroline; Lhoest, Estelle; Hornick, Jean-Luc ULg et al

in Mussa, P. P.; Nery, J.; Schiavone, A. (Eds.) et al Congress Proceedings 13t h Congress of the ESVCN (2009)

Detailed reference viewed: 38 (0 ULg)
Full Text
Peer Reviewed
See detailDevelopment of a predictive model and a risk assessment instrument to facilitate diagnosis of low bone mass in postmenopausal women
Ben Sedrine, Wafa ULg; Chevalier, Th; Micheletti, MC et al

in Arthritis and Rheumatism (2001), 44(1570), 314

Detailed reference viewed: 5 (1 ULg)
Full Text
Peer Reviewed
See detailDevelopment of a procedure to simultaneously isolate RNA, DNA, and proteins from characterizing cells invading or cultured on chitosan scaffolds.
Tchemtchoua Tateu, Victor ULg; Atanasova, Ganka; Aqil, Abdelhafid ULg et al

in Analytical Biochemistry (2009), 393(1), 145-7

For many years, chitosan and its derivatives have been considered to be promising biomaterials for tissue engineering and repair. However, information regarding their biological effect on cell phenotype ... [more ▼]

For many years, chitosan and its derivatives have been considered to be promising biomaterials for tissue engineering and repair. However, information regarding their biological effect on cell phenotype is usually limited to evaluation of cell proliferation and survival, overlooking proteomic and transcriptomic analysis. This is largely related to the lack of efficient and quantitative procedures for protein and nucleic acid purification from cells cultured on, or inside, chitosan scaffold. Here we describe an ultracentrifugation procedure enabling the simultaneous and quantitative recovery of high quality RNA, DNA and proteins from cells growing in close contact of biomaterial matrices containing chitosan. [less ▲]

Detailed reference viewed: 94 (16 ULg)
Full Text
Peer Reviewed
See detailDevelopment of a protocol for micropropagation of Jatropha curcas L
Medza Mve, Samson Daudet ULg; Mergeai, Guy ULg; Baudoin, Jean-Pierre ULg et al

in Fifth International Conference on Renewable Resources and Biorefineries (2009, June 11)

In the present investigation, in vitro clonal propagation of two-month-old Jatropha curcas L. was achieved employing nodal explants. Axillary shoot bud proliferation was best initiated on Murashige and ... [more ▼]

In the present investigation, in vitro clonal propagation of two-month-old Jatropha curcas L. was achieved employing nodal explants. Axillary shoot bud proliferation was best initiated on Murashige and Skoog’s (MS) basal medium supplemented with N6-benzyladenine (BA) and adenine sulphate. This medium allowed the production of 3.1 ± 0.5 shoots per nodal explant with 3.5 ± 0.8 cm average length after 3-4 weeks. [less ▲]

Detailed reference viewed: 22 (5 ULg)
Full Text
Peer Reviewed
See detailDevelopment of a quantitative approach based on surface-enhanced Raman chemical imaging (SER-CI)
De Bleye, Charlotte ULg; Sacre, Pierre-Yves ULg; Dumont, Elodie ULg et al

Conference (2013, October 17)

During the last decade, Raman imaging has taken an important place in the pharmaceutical field [1-2]. It enables to acquire a visual representation of samples while quantifying and identifying molecules ... [more ▼]

During the last decade, Raman imaging has taken an important place in the pharmaceutical field [1-2]. It enables to acquire a visual representation of samples while quantifying and identifying molecules of these samples. However, this technique suffers from a lack of sensitivity and the appearance of fluorescence which can limit its pharmaceutical applications. One way to circumvent these limitations is Surface Enhanced Raman chemical imaging (SER-CI) which presents the advantages of Raman imaging and enables to dramatically increase the Raman scattering of molecules adsorbed or very close to metallic surfaces [3]. The number of publications regarding SER-CI in the pharmaceutical field is very limited probably due to the well-known stability and reproducibility problem of SERS and also due to the difficulty to obtain a homogeneous colloids covering of samples surface before SER-CI analyses. In this context, the possibility to develop a quantitative approach using SER-CI on a pharmaceutical model, presented as paracetamol tablet, was studied. The aim was to develop a SER-CI method enabling to quantify 4-aminophenol (4-AP), which is the main impurity of paracetamol actively research for its toxicity, at a concentration below its limit of specification of 1000 ppm [4]. This pharmaceutical model was first investigated using SERS and a quantitative method enabling to quantify 4-AP from 3 to 15 µg mL-1 was developed and validated [5]. Based on these previous results, the possibility to develop quantitative approach to quantify 4-aminophenol in paracetamol tablet using SER-CI was investigated. Different ways to cover the tablets surface by silver colloids were tested and a homogeneity study was performed in order to improve the repeatability of SER-CI analyses. Afterwards, the SER-CI approach was optimized and different spectral intensity normalizations were tested. Finally, a quantitative approach using SER-CI was developed enabling to quantify 4-AP from 0.025% to 0.2% (w/w) in paracetamol tablets. [less ▲]

Detailed reference viewed: 51 (7 ULg)
Full Text
See detailDevelopment of a quantitative approach to measure phospholipids in dried drops by Raman spectroscopy
Malherbe, Cédric ULg; Jadoul, Laure ULg; Gilbert, Bernard ULg et al

Poster (2013, May 24)

Phospholipids, PL, such as the phosphatidylcholine PC(18:0/18:1), play a role in the structure of living cells and are suspected to be part of the development of some diseases, for example cancers. Mass ... [more ▼]

Phospholipids, PL, such as the phosphatidylcholine PC(18:0/18:1), play a role in the structure of living cells and are suspected to be part of the development of some diseases, for example cancers. Mass spectrometry enables the structural analysis of PL in complex biological media but imaging mass spectrometry by MALDI-MS is rather limited for quantification purposes. Complementarily, Raman spectroscopy as a non invasive and non destructive method is a potential candidate to quantify and visualise the spatial distribution of the PL by molecular imaging. Unfortunately, the lack of specific chemical function in PL, compared to others biomolecules, limits the use of Raman spectroscopy in the identification process of those PL in complex biological samples. The results presented here belong to a first study of the application of the Raman analyses on dried residues of PL and mice brain tissue performed in the lab. [less ▲]

Detailed reference viewed: 49 (16 ULg)
See detailDevelopment of a quantitative approach to measure phospholipids in dried drops by Raman spectroscopy
Malherbe, Cédric ULg; Jadoul, Laure; Gilbert, Bernard ULg et al

Poster (2012, October)

We present here the results obtained during our tentative to analyse quantitatively dried drops of phospholipidic solutions by Raman spectroscopy. Drops of different solutions of phospholipid were deposed ... [more ▼]

We present here the results obtained during our tentative to analyse quantitatively dried drops of phospholipidic solutions by Raman spectroscopy. Drops of different solutions of phospholipid were deposed onto different material supports. The spots were then analyses by confocal Raman microspectroscopy. Experimental settings have been optimised and the analysis of the intensity profile of the Raman signal inside the spot allows the establishment of a calibration curve for the determination of the phospholipids amount within a 1 µL solution. [less ▲]

Detailed reference viewed: 36 (8 ULg)
Full Text
Peer Reviewed
See detailDevelopment of a quantitative approach using surface-enhanced Raman chemical imaging: First step for the determination of an impurity in a pharmaceutical model
De Bleye, Charlotte ULg; Sacre, Pierre-Yves ULg; Dumont, Elodie ULg et al

in Journal of Pharmaceutical & Biomedical Analysis (2014), 90

This publication reports, for the first time, the development of a quantitative approach using surface-enhanced Raman chemical imaging (SER-CI). A pharmaceutical model presented as tablets based on ... [more ▼]

This publication reports, for the first time, the development of a quantitative approach using surface-enhanced Raman chemical imaging (SER-CI). A pharmaceutical model presented as tablets based on paracetamol, which is the most sold drug around the world, was used to develop this approach. 4-Aminophenol is the main impurity of paracetamol and is actively researched in pharmaceutical formulations because of its toxicity. As its concentration is generally very low (<0.1%, w/w), conventional Raman chemical imaging cannot be used. In this context, a SER-CI method was developed to quantify 4-aminophenol assessing a limit of quantification below its limit of specification of 1000 ppm. Citrate-reduced silver nanoparticles were used as SERS substrate and these nanoparticles were functionalized using 1-butanethiol. Different ways to cover the tablets surface by butanethiol-functionalized silver nanoparticles were tested and a homogeneity study of the silver nanoparticles covering was realized. This homogeneity study was performed in order to choose the best way to cover the surface of tablets by silver colloid. Afterwards, the optimization of the SER-CI approach was necessary and different spectral intensity normalizations were tested. Finally, a quantitative approach using SER-CI was developed enabling to quantify 4-aminophenol from 0.025% to 0.2% in paracetamol tablets. This quantitative approach was tested on two different series of tablets using different batches of silver nanoparticles. [less ▲]

Detailed reference viewed: 81 (46 ULg)
Full Text
Peer Reviewed
See detailDevelopment of a quantitative immunocapture real-time PCR assay for detecting structurally intact adenoviral particles in water
Ogorzaly, Leslie; Bonot, Sébastien; Elmoualij, Benaïssa ULg et al

in Journal of Virological Methods (2013), 194

Development of rapid, sensitive and specific methods for detection of infectious enteric viruses in water is challenging but crucial for gaining reliable information for risk assessment. An immunocapture ... [more ▼]

Development of rapid, sensitive and specific methods for detection of infectious enteric viruses in water is challenging but crucial for gaining reliable information for risk assessment. An immunocapture real-time PCR (IC-qPCR) was designed to detect jointly the two major viral particle components, i.e. the capsid protein and the viral genome. Targeting both constituents helps circumventing the technical limits of cell culture approaches and the inability of PCR based methods to predict the infectious status. Two waterborne pathogenic virus models, human adenovirus types 2 and 41, were chosen for this study. IC-qPCR showed a detection limit of 10 MPNCU/reaction with a dynamic range from 102 to 106 MPNCU/reaction. Sensitivity was thus 100-fold higher compared to ELISA-based capture employing the same anti-hexon antibodies. After optimisation, application on environmental water samples was validated, and specificity towards the targeted virus types was obtained through the qPCR step. Heat-treated pure samples as well as surface water samples brought evidence that this method achieves detection of encapsidated viral genomes while excluding free viral genome amplification. As a consequence, adenovirus concentrations estimated by IC-qPCR were below those calculated by direct qPCR. The results demonstrate that the IC-qPCR method is a sensitive and rapid tool for detecting, in a single-tube assay, structurally intact and thus potentially infectious viral particles in environmental samples. [less ▲]

Detailed reference viewed: 47 (4 ULg)
See detailDevelopment of a quantitative method to detect trace amounts of hazelnut and soy allergens in food
Dobson, Rowan ULg; Fourdrilis, Séverine; Kirsch, Stéphanie ULg et al

Conference (2009)

Detailed reference viewed: 24 (3 ULg)
Full Text
Peer Reviewed
See detailDevelopment of a quantitative risk assessment for cheese made from raw goat milk contaminated by Listeria monocytogenes
Delhalle, Laurent ULg; Ellouze, Mariem; Clinquart, Antoine ULg et al

Poster (2011, September)

A retrospective study was performed to assess the potential risk of human listeriosis following a contamination by L. monocytogenes of cheeses made from goat raw milk reported by the Belgian Federal ... [more ▼]

A retrospective study was performed to assess the potential risk of human listeriosis following a contamination by L. monocytogenes of cheeses made from goat raw milk reported by the Belgian Federal Agency for the Safety of the Food Chain in 2005. The source of the contamination was related to a shedder goat, excreting 2.6 log cfu (colonies forming units) L. monocytogenes / ml without any clinical symptom. On the basis of the collected data, a quantitative microbial risk assessment model was developed covering the production chain from the milking of goats until the consumed products. Predictive microbiology models were used to simulate the growth of L. monocytogenes during the process of cheeses made from goat raw milk. The modular exposure assessment model showed a significant growth of L. monocytogenes during chilling and storage of the milk collected the day before the cheese production (increase of 1.7 log cfu/ml for the median) and during the step of starter and rennet adjunction to milk (increase of 0.8 log cfu/ml for the median). The median estimated final result (in the fresh cheese) was equal to 3.5 log cfu/g. The model estimates (expressed as median final result issued from the exposure assessment) were realistic compared to the number of L. monocytogenes measured in the fresh cheese (3.6 log cfu/g) reported during the cheese contamination period. The average number of expected cases of human listeriosis was between 0 and 1 for a high-risk sub-population and 0 for a low-risk healthy sub-population. Scenario analysis was finally performed to identify the most significant factors and aid in developing priorities for risk mitigation. Thus, by using quantitative risk assessment and predictive microbiology models, this study provided valuable information to identify and to control critical steps in a local production chain of goat cheese made from raw milk. [less ▲]

Detailed reference viewed: 65 (5 ULg)
Full Text
Peer Reviewed
See detailDevelopment of a Quick Quantitative Real-Time PCR for the In Vivo Detection and Quantification of Peach latent mosaic viroid
Parisi, Olivier ULg; Lepoivre, Philippe ULg; Jijakli, Haissam ULg

in Plant Disease (2011), 95(2), 137-142

Viroids are plant pathogens infecting a broad range of herbaceous and tree crops. Among them, the Peach latent mosaic viroid (PLMVd) infects mainly peach trees, causing a loss of production with no ... [more ▼]

Viroids are plant pathogens infecting a broad range of herbaceous and tree crops. Among them, the Peach latent mosaic viroid (PLMVd) infects mainly peach trees, causing a loss of production with no curative options. Detecting this viroid is thus important for certification procedures aiming to avoid the release of infected material into orchards. Presented here is a complete detection method based on reverse transcription (RT) followed by a quantitative real-time polymerase chain reaction (PCR). New primers were selected and optimal reaction conditions determined for routine application of the method. The technique is 105 times more sensitive than the endpoint RT-PCR used for PLMVd detection, and permits earlier detection of PLMVd in infected plants. The quick, low-cost extraction procedure used and the quality of the results obtained make this method suitable for routine testing. [less ▲]

Detailed reference viewed: 71 (2 ULg)
Full Text
Peer Reviewed
See detailDevelopment of a radioimmunoassay for bovine chymosin B
Idrissa-Sidikou, Djibo; Remy, Benoit; Dufrasne, Isabelle ULg et al

in Tropicultura (2007), 25(3), 134-139

The present study was conducted to develop and validate a specific radioimmunoassay system for measurement of bovine chymosin B (bChyB) concentrations in plasma samples. Bovine ChyB was used for ... [more ▼]

The present study was conducted to develop and validate a specific radioimmunoassay system for measurement of bovine chymosin B (bChyB) concentrations in plasma samples. Bovine ChyB was used for immunization of rabbits and as standard and tracer. Chymosin B concentrations were measured in plasma samples from two groups of calves (Group 1: calves sampled from birth to 24 hours; Group 2: calves sampled from Day 1 to 21 after birth) and from one cow during the peri-partum period. Detection limit of the assay was 9.0 ng/mL. Recovery was higher than 89.3%. Repeatability and reproducibility ranged from 1.52% to 5.23% and from 1.52% to 12.57% respectively. No cross-reaction was found with pepsinogen A from bovine, porcine or human origins. In Group 1, bChyB concentrations increased from 47.3±45.1 ng/mL (5 min after birth) to 325.5±161.2 ng/mL (12 hours after birth), then it decreased till 293.0±161.5 ng/mL (24 hours after birth). In Group 2, concentrations decreased from Day 1 (455.3±191.1 ng/mL) to Day 21 (117.9±85.1 ng/mL). In adult cow, mean concentration was 136.0±32.3 ng/mL. In conclusion, bChyB is able to cross the stomach basal membrane and to reach the blood circulation at detectable levels in both young calves and adult cows. [less ▲]

Detailed reference viewed: 86 (10 ULg)
Full Text
Peer Reviewed
See detailDevelopment of a Radioimmunoassay for Bovine Pepsinogen A
Sidikou, I. D.; Remy, B.; Beckers, Jean-François ULg

in Revue d'Elévage et de Médecine Vétérinaire des Pays Tropicaux (2005), 58(4), 229-235

Pepsinogen A is the most abundant zymogen found in blood, and its enzymatic measurement is used for the diagnosis of gastric lesions. The present study was conducted to develop a radioimmunoassay (RIA ... [more ▼]

Pepsinogen A is the most abundant zymogen found in blood, and its enzymatic measurement is used for the diagnosis of gastric lesions. The present study was conducted to develop a radioimmunoassay (RIA) specific to pepsinogen A in bovine plasma. The authors purified large amounts of three non-denatured isoforms of bovine pepsinogen A with high proteolytic activity. These homogeneous preparations were used to produce specific antisera in New Zealand White rabbits, and three antisera with high titers were obtained. In the present assay the antiserum #822 was used at a final dilution of 1/250,000. The detection limit of the assay was 20 ng/ml and the recovery ranged from 85.5 to 103.3%. The repeatability (intra-assay coefficient of variation) was lower than 6.6%, whereas the reproducibility (inter-assay coefficient of variation) was lower than 13.4%. The capacity of the RIA to detect pepsinogen A in blood was tested by measuring the concentrations in plasma of newborn calves (n = 6) serially sampled from birth to four months of age. The mean pepsinogen A value (mean ± standard deviation) in the plasma of calves was 2071 ± 752 ng/ml one day after birth. The concentration decreased progressively and was about 1196 ± 307 ng/ml at day 21, and 677 ± 109 ng/ml at day 120. The present study is the first report on pepsinogen A concentrations in bovine measured by RIA. Further investigations using the RIA should be performed in order to confirm these values and determine pepsinogen levels in older cattle in physiological and pathological conditions such as gastrointestinal helminthosis [less ▲]

Detailed reference viewed: 14 (1 ULg)
Full Text
See detailDevelopment of a RAPD marker and a semi-selective medium for aureobasidium pullulans (strain Ach1-1), a biocontrol agent against postharvest disease on apples
El Hamouchi, A.; Najimi, B.; Achbani, E. H. et al

in Bulletin OILB/SROP = IOBC/WPRS Bulletin (2007), 30

Detailed reference viewed: 22 (0 ULg)
Full Text
Peer Reviewed
See detailDevelopment Of A Rapid Rt-Pcr Test For The Detection Of Peach Latent Mosaic Viroid, Pear Blister Canker Viroid, Hop Stunt Viroid And Apple Scar Skin Viroid In Fruit Trees From Tunisia
Hassen, If.; Roussel, S.; Kummert, J. et al

in Journal of Phytopathology (2006), 154(4), 217-223

Detailed reference viewed: 9 (0 ULg)
See detailDevelopment of a regenerable filter aid for beverages
Bonacchelli, B.; Zgoulli, S.; Harmegnies, F. et al

Poster (1998, May 08)

Detailed reference viewed: 27 (0 ULg)