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Peer Reviewed
See detailDevelopment of a parenteral and of an oral formulation of albendazole with cyclodextrins
Piel, Géraldine ULg; Evrard, Brigitte ULg; Van Hees, Thierry ULg et al

in STP Pharma Sciences (1999), 3

L'albendazole est une dérivé benzimidazole à large spectre d'activité contre les parasites helminthiques de l'homme et de l'animal. L'albendazole est très faiblement soluble dans l'eau. Il a été démontré ... [more ▼]

L'albendazole est une dérivé benzimidazole à large spectre d'activité contre les parasites helminthiques de l'homme et de l'animal. L'albendazole est très faiblement soluble dans l'eau. Il a été démontré que les cyclodextrines sont capables de former des complexes d'inclusion avec l'albendazole et d'en augmenter la solubilité. La solubilité aqueuse de l'albendazole est dépendante du pH. Le but de ce travail est d'étudier l'influence combinée des cyclodextrines et de différents acides sur la solubilité de l'albendazole. Il a été démontré qu'il existe un véritable effet synergique entre les cyclodextrines et les différents acides. L'utilisation combinée d'hydroxypropyl-b-cyclodextrine (200 mM) et d'acide lactique ou acide citrique (50 mM) permet de dissoudre plus de 1 mg/ml d'albendazole. Des études RMN ont démontré l'inclusion de l'albendazole dans la b-cyclodextrine en milieu acide. Ces résultats montrent qu'il est possible de réaliser une solution injectable et une solution orale d'albendazole grâce aux cyclodextrines. [less ▲]

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See detailDevelopment of a performant method for glucocapparin determination in Boscia senegalensis Lam ex. Poir. : A study of the variability
Gueye, Momar Talla; Seck, Dogo; Diallo, Abdoulaye et al

in American Journal of Analytical Chemistry (2013), 4

This study describes a glucocapparin determination method. Based on rapeseed determination of glucosinolate (GSL), the equation of the average straight regression line is Y = 100.42X − 0.03 (R2 = 0.9998 ... [more ▼]

This study describes a glucocapparin determination method. Based on rapeseed determination of glucosinolate (GSL), the equation of the average straight regression line is Y = 100.42X − 0.03 (R2 = 0.9998). Enzymatic hydrolysis of glucocapparin extracted from leaves and fruits of B. senegalensis, analyzed by SPME-GC-MS confirmed the presence of methylisothiocyanate as the main hydrolysis glucocapparin product. Monitoring glucocapparin contents in B. senegalensis leaves and fruits collected in 4 localities in Senegal showed differences between organs according localities and periods of harvest. Glucocapparin content was very high in dry season particularly in January and the lowest rates were recorded during the rainy period between August and November. [less ▲]

Detailed reference viewed: 72 (14 ULg)
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See detailDevelopment of a pharmacological protocol of stress echocardiography in horses
Sandersen, Charlotte ULg

in Annales de Médecine Vétérinaire (2008)

Detailed reference viewed: 17 (4 ULg)
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See detailDevelopment of a positron emission tomography radiopharmaceutical for imaging thymidine kinase gene expression : Synthesis and in vitro evaluation of 9-{3-[18F] Fluoro-1-hydroxy-2-propoxymethyl guanine
Monclus, Michel; Luxen, André ULg; Cool, Vincent et al

in Bioorganic and Medicinal Chemistry Letters (1997), 7(14), 1879-1882

9-[(3-fluoro-1-hydroxy-2-propoxy)methyl]guanine (FHPG) 2 has been labeled with fluorine-18 and evaluated in vitro as a potential radiotracer for mapping gene expression in vivo with positron emission ... [more ▼]

9-[(3-fluoro-1-hydroxy-2-propoxy)methyl]guanine (FHPG) 2 has been labeled with fluorine-18 and evaluated in vitro as a potential radiotracer for mapping gene expression in vivo with positron emission tomography (PET). [less ▲]

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See detailDevelopment of a practical test of insulin resistance in obese Beagle dogs and effects of sc FOS
Daumas, Caroline; Lhoest, Estelle; Hornick, Jean-Luc ULg et al

in Mussa, P. P.; Nery, J.; Schiavone, A. (Eds.) et al Congress Proceedings 13t h Congress of the ESVCN (2009)

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See detailDevelopment of a predictive model and a risk assessment instrument to facilitate diagnosis of low bone mass in postmenopausal women
Ben Sedrine, Wafa ULg; Chevalier, Th; Micheletti, MC et al

in Arthritis and Rheumatism (2001), 44(1570), 314

Detailed reference viewed: 5 (1 ULg)
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See detailDevelopment of a procedure to simultaneously isolate RNA, DNA, and proteins from characterizing cells invading or cultured on chitosan scaffolds.
Tchemtchoua Tateu, Victor ULg; Atanasova, Ganka; Aqil, Abdelhafid ULg et al

in Analytical Biochemistry (2009), 393(1), 145-7

For many years, chitosan and its derivatives have been considered to be promising biomaterials for tissue engineering and repair. However, information regarding their biological effect on cell phenotype ... [more ▼]

For many years, chitosan and its derivatives have been considered to be promising biomaterials for tissue engineering and repair. However, information regarding their biological effect on cell phenotype is usually limited to evaluation of cell proliferation and survival, overlooking proteomic and transcriptomic analysis. This is largely related to the lack of efficient and quantitative procedures for protein and nucleic acid purification from cells cultured on, or inside, chitosan scaffold. Here we describe an ultracentrifugation procedure enabling the simultaneous and quantitative recovery of high quality RNA, DNA and proteins from cells growing in close contact of biomaterial matrices containing chitosan. [less ▲]

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See detailDevelopment of a protocol for micropropagation of Jatropha curcas L
Medza Mve, Samson Daudet ULg; Mergeai, Guy ULg; Baudoin, Jean-Pierre ULg et al

in Fifth International Conference on Renewable Resources and Biorefineries (2009, June 11)

In the present investigation, in vitro clonal propagation of two-month-old Jatropha curcas L. was achieved employing nodal explants. Axillary shoot bud proliferation was best initiated on Murashige and ... [more ▼]

In the present investigation, in vitro clonal propagation of two-month-old Jatropha curcas L. was achieved employing nodal explants. Axillary shoot bud proliferation was best initiated on Murashige and Skoog’s (MS) basal medium supplemented with N6-benzyladenine (BA) and adenine sulphate. This medium allowed the production of 3.1 ± 0.5 shoots per nodal explant with 3.5 ± 0.8 cm average length after 3-4 weeks. [less ▲]

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See detailDevelopment of a quantitative approach based on surface-enhanced Raman chemical imaging (SER-CI)
De Bleye, Charlotte ULg; Sacre, Pierre-Yves ULg; Dumont, Elodie ULg et al

Conference (2013, October 17)

During the last decade, Raman imaging has taken an important place in the pharmaceutical field [1-2]. It enables to acquire a visual representation of samples while quantifying and identifying molecules ... [more ▼]

During the last decade, Raman imaging has taken an important place in the pharmaceutical field [1-2]. It enables to acquire a visual representation of samples while quantifying and identifying molecules of these samples. However, this technique suffers from a lack of sensitivity and the appearance of fluorescence which can limit its pharmaceutical applications. One way to circumvent these limitations is Surface Enhanced Raman chemical imaging (SER-CI) which presents the advantages of Raman imaging and enables to dramatically increase the Raman scattering of molecules adsorbed or very close to metallic surfaces [3]. The number of publications regarding SER-CI in the pharmaceutical field is very limited probably due to the well-known stability and reproducibility problem of SERS and also due to the difficulty to obtain a homogeneous colloids covering of samples surface before SER-CI analyses. In this context, the possibility to develop a quantitative approach using SER-CI on a pharmaceutical model, presented as paracetamol tablet, was studied. The aim was to develop a SER-CI method enabling to quantify 4-aminophenol (4-AP), which is the main impurity of paracetamol actively research for its toxicity, at a concentration below its limit of specification of 1000 ppm [4]. This pharmaceutical model was first investigated using SERS and a quantitative method enabling to quantify 4-AP from 3 to 15 µg mL-1 was developed and validated [5]. Based on these previous results, the possibility to develop quantitative approach to quantify 4-aminophenol in paracetamol tablet using SER-CI was investigated. Different ways to cover the tablets surface by silver colloids were tested and a homogeneity study was performed in order to improve the repeatability of SER-CI analyses. Afterwards, the SER-CI approach was optimized and different spectral intensity normalizations were tested. Finally, a quantitative approach using SER-CI was developed enabling to quantify 4-AP from 0.025% to 0.2% (w/w) in paracetamol tablets. [less ▲]

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See detailDevelopment of a quantitative approach to measure phospholipids in dried drops by Raman spectroscopy
Malherbe, Cédric ULg; Jadoul, Laure ULg; Gilbert, Bernard ULg et al

Poster (2013, May 24)

Phospholipids, PL, such as the phosphatidylcholine PC(18:0/18:1), play a role in the structure of living cells and are suspected to be part of the development of some diseases, for example cancers. Mass ... [more ▼]

Phospholipids, PL, such as the phosphatidylcholine PC(18:0/18:1), play a role in the structure of living cells and are suspected to be part of the development of some diseases, for example cancers. Mass spectrometry enables the structural analysis of PL in complex biological media but imaging mass spectrometry by MALDI-MS is rather limited for quantification purposes. Complementarily, Raman spectroscopy as a non invasive and non destructive method is a potential candidate to quantify and visualise the spatial distribution of the PL by molecular imaging. Unfortunately, the lack of specific chemical function in PL, compared to others biomolecules, limits the use of Raman spectroscopy in the identification process of those PL in complex biological samples. The results presented here belong to a first study of the application of the Raman analyses on dried residues of PL and mice brain tissue performed in the lab. [less ▲]

Detailed reference viewed: 49 (16 ULg)
See detailDevelopment of a quantitative approach to measure phospholipids in dried drops by Raman spectroscopy
Malherbe, Cédric ULg; Jadoul, Laure; Gilbert, Bernard ULg et al

Poster (2012, October)

We present here the results obtained during our tentative to analyse quantitatively dried drops of phospholipidic solutions by Raman spectroscopy. Drops of different solutions of phospholipid were deposed ... [more ▼]

We present here the results obtained during our tentative to analyse quantitatively dried drops of phospholipidic solutions by Raman spectroscopy. Drops of different solutions of phospholipid were deposed onto different material supports. The spots were then analyses by confocal Raman microspectroscopy. Experimental settings have been optimised and the analysis of the intensity profile of the Raman signal inside the spot allows the establishment of a calibration curve for the determination of the phospholipids amount within a 1 µL solution. [less ▲]

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Peer Reviewed
See detailDevelopment of a quantitative approach using surface-enhanced Raman chemical imaging: First step for the determination of an impurity in a pharmaceutical model
De Bleye, Charlotte ULg; Sacre, Pierre-Yves ULg; Dumont, Elodie ULg et al

in Journal of Pharmaceutical & Biomedical Analysis (2014), 90

This publication reports, for the first time, the development of a quantitative approach using surface-enhanced Raman chemical imaging (SER-CI). A pharmaceutical model presented as tablets based on ... [more ▼]

This publication reports, for the first time, the development of a quantitative approach using surface-enhanced Raman chemical imaging (SER-CI). A pharmaceutical model presented as tablets based on paracetamol, which is the most sold drug around the world, was used to develop this approach. 4-Aminophenol is the main impurity of paracetamol and is actively researched in pharmaceutical formulations because of its toxicity. As its concentration is generally very low (<0.1%, w/w), conventional Raman chemical imaging cannot be used. In this context, a SER-CI method was developed to quantify 4-aminophenol assessing a limit of quantification below its limit of specification of 1000 ppm. Citrate-reduced silver nanoparticles were used as SERS substrate and these nanoparticles were functionalized using 1-butanethiol. Different ways to cover the tablets surface by butanethiol-functionalized silver nanoparticles were tested and a homogeneity study of the silver nanoparticles covering was realized. This homogeneity study was performed in order to choose the best way to cover the surface of tablets by silver colloid. Afterwards, the optimization of the SER-CI approach was necessary and different spectral intensity normalizations were tested. Finally, a quantitative approach using SER-CI was developed enabling to quantify 4-aminophenol from 0.025% to 0.2% in paracetamol tablets. This quantitative approach was tested on two different series of tablets using different batches of silver nanoparticles. [less ▲]

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See detailDevelopment of a quantitative immunocapture real-time PCR assay for detecting structurally intact adenoviral particles in water
Ogorzaly, Leslie; Bonot, Sébastien; Elmoualij, Benaïssa ULg et al

in Journal of Virological Methods (2013), 194

Development of rapid, sensitive and specific methods for detection of infectious enteric viruses in water is challenging but crucial for gaining reliable information for risk assessment. An immunocapture ... [more ▼]

Development of rapid, sensitive and specific methods for detection of infectious enteric viruses in water is challenging but crucial for gaining reliable information for risk assessment. An immunocapture real-time PCR (IC-qPCR) was designed to detect jointly the two major viral particle components, i.e. the capsid protein and the viral genome. Targeting both constituents helps circumventing the technical limits of cell culture approaches and the inability of PCR based methods to predict the infectious status. Two waterborne pathogenic virus models, human adenovirus types 2 and 41, were chosen for this study. IC-qPCR showed a detection limit of 10 MPNCU/reaction with a dynamic range from 102 to 106 MPNCU/reaction. Sensitivity was thus 100-fold higher compared to ELISA-based capture employing the same anti-hexon antibodies. After optimisation, application on environmental water samples was validated, and specificity towards the targeted virus types was obtained through the qPCR step. Heat-treated pure samples as well as surface water samples brought evidence that this method achieves detection of encapsidated viral genomes while excluding free viral genome amplification. As a consequence, adenovirus concentrations estimated by IC-qPCR were below those calculated by direct qPCR. The results demonstrate that the IC-qPCR method is a sensitive and rapid tool for detecting, in a single-tube assay, structurally intact and thus potentially infectious viral particles in environmental samples. [less ▲]

Detailed reference viewed: 47 (4 ULg)
See detailDevelopment of a quantitative method to detect trace amounts of hazelnut and soy allergens in food
Dobson, Rowan ULg; Fourdrilis, Séverine; Kirsch, Stéphanie ULg et al

Conference (2009)

Detailed reference viewed: 24 (3 ULg)