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See detailDetection of new biallelic polymorphisms in the human MxA gene
Tran Thi Duc, Tam; Farnir, Frédéric ULg; Michaux, Charles ULg et al

in Molecular Biology Reports (2012), 39(8), 8533-8538

The interferon-inducible human MxA protein plays an important role in innate defense against an array of viruses. One might expect allelic diversity at the MxA locus to influence the timing and magnitude ... [more ▼]

The interferon-inducible human MxA protein plays an important role in innate defense against an array of viruses. One might expect allelic diversity at the MxA locus to influence the timing and magnitude of its expression or even the range of viruses whose biological cycle is inhibited by the encoded product. Here we have collected 267 samples of genomic DNA from three distinct populations (European, Asian, and African) and have systematically sequenced the promoter of the MxA gene and its 17 exons in order to inventory its allelic variants. Fifteen single-nucleotide polymorphisms were detected, four of which had never been identified before. Two of these, located in the promoter (at positions -309 and -101 respectively), might affect the MxA expression pattern. The other two result in substitutions (Gly255Glu and Val268Met) in the protein’s N-terminal region that might directly affect its antiviral function. [less ▲]

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See detailDetection of newly-synthetized RNAs in cell nuclei by a non-isotopic method using bromouridine
Nizet, S; Thiry, Marc ULg; Goessens, G

Poster (1997)

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See detailDetection of nitric oxide by diaminofluorescein visualizes the skeleton in living zebrafish.
Renn, Jörg ULg; Pruvot, Benoist; Muller, Marc ULg

in Journal of Applied Ichthyology (2014), 30(4), 701706

Several in vivo stainings, such as Calcein, Alizarin Red and Quercetin are commonly used to visualize ossification in living teleost specimen. These staining techniques represent important tools for bone ... [more ▼]

Several in vivo stainings, such as Calcein, Alizarin Red and Quercetin are commonly used to visualize ossification in living teleost specimen. These staining techniques represent important tools for bone research in fish, but do not visualize cartilage. In the present study, we show that nitric oxide (NO) labelling by DAF-FM DA visualizes both bone and cartilage in vivo during zebrafish skeletogenesis. NO detection performed in Tg(osterix:mCherry) or in combination with Alizarin Red in wild-type zebrafish reveals that intense staining through NO labelling colocalizes with the appearance of osteoblasts and characterizes ossified structures. Cartilage structures are clearly distinguished in the living larvae, although the labelling is less intensive when compared to ossified structures. This method is the first and easy to handle alternative to cartilage and bone double stainings on fixed samples. In contrast to most live skeletal stainings, which only stain the mineralized bone structures, this protocol in addition allows in vivo visualization of cartilage. [less ▲]

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See detailDetection of non concave and non increasing multifractal spectra using wavelet leaders (Part I)
Esser, Céline ULg; Kleyntssens, Thomas ULg; Bastin, Françoise ULg et al

Conference (2014, May 22)

Multifractal analysis is concerned with the study of very irregular signals. For such functions, the pointwise regularity may change widely from a point to another. Therefore, it is more interesting to ... [more ▼]

Multifractal analysis is concerned with the study of very irregular signals. For such functions, the pointwise regularity may change widely from a point to another. Therefore, it is more interesting to determine the spectrum of singularities of the signal, which is the Hausdor ff dimension of the set of points which have the same H ölder exponent. For real-life signals, the computation of the spectrum of singularities from its de finition is not feasible. Multifractal formalisms are used to approximate this spectrum. Currently, there exist several methods. In this talk, we present a new multifractal formalism based on the wavelet leaders of a signal which allows to detect non concave and non increasing spectra. [less ▲]

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See detailDetection of non concave and non increasing multifractal spectra using wavelet leaders (Part II)
Kleyntssens, Thomas ULg; Esser, Céline ULg; Nicolay, Samuel ULg

Conference (2014, May 22)

This talk follows "Detection of non concave and non increasing multifractal spectra using wavelet leaders (Part I)" given by Céline Esser. A multifractal formalism is a numerically computable formula that ... [more ▼]

This talk follows "Detection of non concave and non increasing multifractal spectra using wavelet leaders (Part I)" given by Céline Esser. A multifractal formalism is a numerically computable formula that approximates the spectrum of singularities of a function. A new multifractal formalism based on the wavelet leaders is presented as well as a comparison with other formalisms. Its main advantages are that it allows to detect non concave and non increasing spectra. An implementation is proposed. [less ▲]

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See detailDetection of non-concave and non-increasing spectra: Snu spaces revisited with wavelet leaders
Esser, Céline ULg

Conference (2014, September 26)

Our objective is to study the pointwise regularity of functions via their multifractal spectrum. Computing the multifractal spectrum of a function using directly its definition is an unattainable goal in ... [more ▼]

Our objective is to study the pointwise regularity of functions via their multifractal spectrum. Computing the multifractal spectrum of a function using directly its definition is an unattainable goal in most of the practical cases, but there exist heuristic methods, called multifractal formalisms, which allow to estimate this spectrum and which give satisfactory results in many situations. The Frisch-Parisi conjecture, classically used and based on Besov spaces, presents two disadvantages: it can only hold for spectra that are concave and it can only yield the increasing part of spectra. Concerning the first problem, the use of S spaces allows to deal with non-concave increasing spectra. Concerning the second problem, a generalization of the Frisch-Parisi conjecture obtained by replacing the role played by wavelet coefficients by wavelet leaders allows to recover the decreasing part of concave spectra. We present a combination of both approaches to define a new formalism derived from large deviations based on statistics of wavelet leaders. We also present the associated function space. [less ▲]

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See detailDetection of nonlinearity in a dynamic system using deformation modes obtained from the wavelet transform of measured responses
Nguyen, Viet Ha ULg; Peeters, Maxime ULg; Golinval, Jean-Claude ULg

in Shock and Vibration (2010), 17(4-5), 491-506

An efficient approach to Structural Health Monitoring of dynamical systems based on the Wavelet Transform (WT) and the concept of subspace angle is presented. The objective is to propose a detection ... [more ▼]

An efficient approach to Structural Health Monitoring of dynamical systems based on the Wavelet Transform (WT) and the concept of subspace angle is presented. The objective is to propose a detection method that is sensitive to the onset of nonlinear behaviour in a dynamic system. For this purpose, instantaneous frequencies are identified first from output-only vibration signals using the Wavelet Transform. Time varying deformation shapes are then extracted by analyzing the whole measurement data set on the structure. From this information, different dynamic states of the structure may be detected by inspecting time variations of 'modal' features. The experimental structure considered here as application example is a clamped beam with a geometric nonlinearity. Detection of nonlinearity is carried out by means of the concept of subspace angles between instantaneous deformation modes extracted from measurement data using the continuous Wavelet Transform. The method consists in controlling the angular coherence between active subspaces of the current and reference states respectively. The proposed technique, which shows a good sensitivity to small changes in the dynamic behaviour of the structure, may also be used for damage detection. [less ▲]

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See detailDetection of nonlinearity in a dynamic system using deformation modes obtained from the Wavelet Transfrom of measured responses
Nguyen, Viet Ha ULg; Peeters, Maxime ULg; Golinval, Jean-Claude ULg

Conference given outside the academic context (2009)

An efficient approach to Structural Health Monitoring of dynamical systems based on the Wavelet Transform (WT) and the concept of subspace angle is presented. The objective is to propose a detection ... [more ▼]

An efficient approach to Structural Health Monitoring of dynamical systems based on the Wavelet Transform (WT) and the concept of subspace angle is presented. The objective is to propose a detection method that is sensitive to the onset of nonlinear behaviour in a dynamic system. For this purpose, instantaneous frequencies are identified first from output-only vibration signals using the Wavelet Transform. Time varying deformation shapes are then extracted by analyzing the whole measurement data set on the structure. From this information, different dynamic states of the structure may be detected by inspecting time variations of ‘modal’ features. The experimental structure considered here as application example is a clamped beam with a geometric nonlinearity. Detection of nonlinearity is carried out by means of the concept of subspace angles between instantaneous deformation modes extracted from measurement data using the continuous Wavelet Transform. The method consists in controlling the angular coherence between active subspaces of the current and reference states respectively. The proposed technique, which shows a good sensitivity to small changes in the dynamic behaviour of the structure, may also be used for damage detection. [less ▲]

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See detailDetection of novel single-nucleotide polymorphisms (SNPs) in the CYP21 gene and association analysis of two SNPs for CYP21 and ESR2 with litter size in a commercial sow population
Buske, Bernd ULg; Sternstein, Ina; Reissmann, Monika et al

in Journal of Animal Breeding & Genetics (2006), 123(5), 343-348

Detailed reference viewed: 38 (11 ULg)
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See detailDetection of nucleic acids by immunocytological approaches
Thiry, Marc ULg

in Acta Microscopica (1996), 5

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See detailDetection of nucleic acids by in situ molecular immunocytochemistry
Thiry, Marc ULg

in Histochemical Journal (The) (1992), 24

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See detailDetection of Nucleic Acids in the Attomole Range Using Polybiotinylated Oligonucleotide Probes
Zecchini, Vincent; Caucheteux, Danièle; Gretry, Jeau et al

in BioTechniques (1995), 19(2), 286-90

This article describes the optimization of the hybridization signal obtained with biotinylated oligonucleotides. Optimal number and positions of biotin moieties on a 33-base oligonucleotide probe were ... [more ▼]

This article describes the optimization of the hybridization signal obtained with biotinylated oligonucleotides. Optimal number and positions of biotin moieties on a 33-base oligonucleotide probe were determined. The quality of avidin-peroxidase conjugate and the choice of chromogenic substrate influenced detection sensitivity. A signal amplification method was also developed for avidin enzymatic conjugates. These improvements allowed the detection of less than 0.02 fmol of target DNA. [less ▲]

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See detailDetection of Oligonucleotide Gas-Phase Conformers: H/D Exchange and Ion Mobility as Complementary Techniques
Balbeur, Dorothée ULg; Widart, Joëlle ULg; Leyh, Bernard ULg et al

in Journal of the American Society for Mass Spectrometry (2008), 19(7), 938-946

Gas-phase hydrogen/deuterium exchange of small oligonucleotides (dTG, dC6 and C6) with CD3OD was performed in the second hexapole of a Fourier transform ion-cyclotron resonance (FTICR) mass spectrometer ... [more ▼]

Gas-phase hydrogen/deuterium exchange of small oligonucleotides (dTG, dC6 and C6) with CD3OD was performed in the second hexapole of a Fourier transform ion-cyclotron resonance (FTICR) mass spectrometer. Ion activation experiments were conducted by accelerating the ions at the entrance of the H/D exchange cell under conditions promoting exclusively collisional isomerization. These experiments allowed us to assess the presence of several conformers, and to probe the height of the isomerization barrier separating these conformers. Ion mobility experiments were also performed. Their results were consistent with the H/D exchange data. A model accounting for the competing isomerization and H/D exchange reactions is proposed. Comparing the ion acceleration experiments for H/D exchange and for ion mobility reveals that the most compact conformer displays the fastest H/D exchange. This observation shows that H/D exchange and ion mobility provide us with complementary information because hydrogen accessibility and macromolecule compactness are not univocally associated. [less ▲]

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See detailDetection of past and future atmospheric circulation changes over the North Atlantic region with the help of an automatic circulation type classification
Belleflamme, Alexandre ULg

Doctoral thesis (2015)

Future projections of the atmospheric circulation over the Northern Hemisphere high latitudes, especially the North Atlantic, have high uncertainties and some of the projected changes are opposed to ... [more ▼]

Future projections of the atmospheric circulation over the Northern Hemisphere high latitudes, especially the North Atlantic, have high uncertainties and some of the projected changes are opposed to circulation changes that have been observed since the 2000s. In this thesis, we focus on three particular aspects of the past and projected future summertime atmospheric circulation over the broader North Atlantic region. First, we analyse whether the 2007-2012 summertime anticyclonic anomaly over the Beaufort Sea, the Canadian Arctic Archipelago, and Greenland might rather be due to global warming or to the internal variability of the atmospheric circulation by putting it in perspective with the circulation variability over the last 150 years given by five reanalysis datasets. Then, this analysis is extended for the future circulation projected towards 2100 by CMIP3 and CMIP5 General Circulation Models (GCMs) over Greenland. Finally, we evaluate the impact of the uncertainties of the future atmospheric circulation projections on the mitigating or enhancing influence of the summertime circulation changes on temperature and precipitation over Europe. We use an automatic circulation type classification to analyse in detail the atmospheric circulation changes by grouping similar daily SLP (mean sea level pressure) or Z500 (500 hPa geopotential height) fields into homogeneous circulation types. It appears that the choice of the index, on the basis of which the days are grouped together, strongly influences the characteristics of the circulation types and the kinds of circulation changes that can be detected. In comparison with Euclidean distance and pressure gradient-based indices, correlation-based indices, especially the Spearman rank correlation, are the most suitable indices when focusing on the circulation pattern. Over the Arctic region, four periods with circulation anomalies similar to that of 2007-2012 (i.e. a summertime anticyclonic anomaly over the western Arctic region) have been detected over the last 150 years, despite a higher uncertainty of the circulation given by the reanalyses due to the scarcity of observational data before 1940. Nevertheless, the 2007-2012 anomaly appears to be exceptional and several connexions with other variables, such as the North Atlantic Oscillation index and sea ice loss, suggest that it could be part of a major climatic anomaly extending beyond the Arctic region. However, the occurrence of similar periods in the past and the influence of several external and internal forcings do not allow us to attribute it to global warming. The future summertime atmospheric circulation projected by GCMs over Greenland confirms this conclusion. In fact, no significant circulation pattern changes are simulated towards 2100, besides a generalised Z500 increase caused by the projected warming. Since GCMs are able to simulate atmospheric circulation changes over other regions and since the atmospheric circulation itself is influenced by other variables, such as sea ice or snow extent, which are already impacted by long-term changes, we conclude that the 2007-2012 anomaly could rather be attributed to the internal variability of the climatic system. Finally, we evidence that projected future atmospheric circulation changes impact on the SLP and precipitation changes simulated over Europe towards 2100 for summer. Over north-western Europe, these circulation changes could mitigate the SLP decrease by around 50 % and cancel out the precipitation increase. Nevertheless, high uncertainties among the GCMs on the magnitude and even on the sign of these changes cast doubt on the reliability of these projections. On the other hand, future atmospheric circulation changes are not projected to affect significantly the warming and drying simulated for the next decades over the Mediterranean region and eastern Europe. [less ▲]

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See detailDetection of placental lactogens in swamp buffalo by radioimmunoassay technique
Hanh, N. V.; Huu, Q. X.; Uoc, N. T. et al

in Reproduction, Fertility and Development (2009), 21(1), 152-153

Ruminant placental lactogens (PL) are members of the growth factor/prolactin (GH/PRL) family. They are synthesized by trophectodermal binucleate cells. There is evidence to suggest that PL is involved in ... [more ▼]

Ruminant placental lactogens (PL) are members of the growth factor/prolactin (GH/PRL) family. They are synthesized by trophectodermal binucleate cells. There is evidence to suggest that PL is involved in control of fetal growth, through actions in both the maternal and fetal compartments, as well as in influencing mammary growth during pregnancy (Byatt JC et al. 1992 J. Anim. Sci. 70, 2911–2923). The structure and biology of PL have been studied in the cow, sheep, goat, human, and mice. The maternal concentration of PL is 100- to 1 000-fold greater in pregnant sheep and goats than in cows but no information exists about PL concentration in buffalo. The aim of the present study was to evaluate the ability to detect PL in buffalo fluids by using bovine PL antibody. Samples were collected in the slaughterhouse immediately after animal slaughter. The fetuses were measured after heart blood collection. A bPL RIA system was used to determine the bPL concentrations in the buffalo samples (Alvarez-Oxiley AV et al. 2007 Reprod. Fertil. Dev. 19, 877–885). The rbPL molecules were radio-iodinated with [125]I-Na by using the lactoperoxidase method (Thorell JI and Johansson BG 1971 Biochim. Biophys. Acta 251, 363–369). Concentrations of buffalo PL are presented in Table 1. In this RIA system, the minimum detected value was 0.068 ng mL–1, and the binding competition curves of bovine PL standard and buffalo fluids dilution using bovine PL antibody were paralleled in all kinds of samples. The lowest concentration was detected in allantoid fluid and the greatest concentration in fetal plasma (P < 0.05). Study of the biology of PL in buffalo has proved difficult because the concentration of PL in all buffalo fluids is very low. Furthermore, the research concerning buffalo PL function required in vivo experiments. Existing data suggest that at least the concentration of buffalo PL is different from cattle and other smaller domestic ruminants. In conclusion, our results provide preliminary information about concentrations of PL in buffalo fluids. [less ▲]

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See detailDetection of placental lactogens in Swamp buffalo by radioimmunoassay technique.
Hanh, N. V.; Huu, Q. X.; Uoc, N. T. et al

in Reproduction, Fertility and Development (2009), 21(1), 152-153

Detailed reference viewed: 7 (3 ULg)
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See detailDetection of plant viruses by molecular hybridization using non-radioactive probes.
Kummert, J.; Colinet, D.; Lepoivre, Philippe ULg

in Bulletin OEPP (1995), 25(1-2),

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See detailDetection of plant viruses by PCR using group or virus specific primers (potyviruses, bymoviruses).
Colinet, D.; Kummert, J.; Duterme, O. et al

in Bulletin OEPP (1995), 25(1-2),

Detailed reference viewed: 28 (0 ULg)