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See detailDevelopment of pH–responsive nanocarriers using trimethylchitosans and methacrylic acid copolymer for siRNA delivery
Dehousse, Vincent ULg; Garbacki, Nancy ULg; Colige, Alain ULg et al

in Biomaterials (2010), 31

RNA interference-based therapies are dependent on intracellular delivery of siRNA. The release of siRNA from the endosomal compartment may be a rate limiting step in the transfection process. The purpose ... [more ▼]

RNA interference-based therapies are dependent on intracellular delivery of siRNA. The release of siRNA from the endosomal compartment may be a rate limiting step in the transfection process. The purpose of this study was to produce pH–responsive nanocarriers made of trimethylchitosan (TMC). To this end, pHsensitive methacrylic acid (MAA) copolymer was added to TMC–siRNA formulations. Four different TMCs associated or not with MAA were evaluated as siRNA carriers. Nanoparticles were characterized in terms of size, surface charge, morphology and interaction with siRNA. A swelling behaviour due to a decrease in pH was observed and was found to be dependent on MAA content in the complexes. In vitro experiments aimed at evaluating how the capacity of the nanocarriers to transfect siRNA in L929 cells was affected by MAA content. Confocal microscopy experiments showed that fluorescent MAA-containing particles exhibit a different distribution pattern inside the cells comparing to their counterpart without this pH-sensitive polymer. Transfection efficiency was investigated by RhoA mRNA expression inhibition. MAA–TMC–siRNA complexes were able to transfect L929 cells with greater efficiency than corresponding TMC–siRNA complexes. This study gives an insight into the opportunity of pH-sensitive nanocarriers for siRNA delivery. Such formulations may represent an attractive strategy to improve endosomal escape of siRNA. [less ▲]

Detailed reference viewed: 61 (21 ULg)
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See detailDevelopment of pilot Trans-European service (Rhine-Main-Danube) for combined freight transport between Walloon region and the Black Sea
Marchal, Jean ULg; Kicheva, D.; Petkov, N. et al

in Eigth international conference on Marine sciences and Technologies “Balack Sea 2006” (2006, September)

Detailed reference viewed: 21 (0 ULg)
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See detailDevelopment of Procedural Learning in Children : The “Toy Task”
Lejeune, Caroline ULg; Meulemans, Thierry ULg

Poster (2008)

The authors generally agree that procedural learning abilities play an important role in the child development and that they are present early in childhood. However, few studies have been carried out in ... [more ▼]

The authors generally agree that procedural learning abilities play an important role in the child development and that they are present early in childhood. However, few studies have been carried out in this area because of the difficulty to develop tests specifically designed for the evaluation of these capacities in young children. In this study, we presented a new task aimed to evaluate visuo-motor skill learning in the young child. The purpose of the present study was to explore whether procedural learning abilities are present in the same extent in 4-year as 7- and 10-year-old children. Our sample is composed of 60 children divided into 3 age-groups. The task is composed of 5 blocks of 12 trials during which each subject has to use a reversed mouse of a computer in order to “catch” different toys appearing on the screen; 2 blocks of 12 trials was administered after a 15 minutes delay. Our results show an important difference between the groups for the first block; on the other hand, for the next blocks, the results show an absence of age-related difference between participants in our perceptive-motor skill learning task, confirming the idea according to which procedural learning abilities are efficient early in development (the difference observed during the first block being probably due to the intervention of executive mechanisms). [less ▲]

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See detailDevelopment of process control system for potential use of direct injection spraying technology
Aissaoui, Abdellah; Lebeau, Frédéric ULg; el bahir, et al

in Progress in Agricultural Engineering Sciences (2011), 7(1), 33-45

Small-scale farmers face to actual difficulties of applying pesticides accurately and safely on vegetables crops. They mainly use hand operated sprayers. As an issue, a small direct injection system based ... [more ▼]

Small-scale farmers face to actual difficulties of applying pesticides accurately and safely on vegetables crops. They mainly use hand operated sprayers. As an issue, a small direct injection system based on a five meter’s parallel boom layout was designed to improve chemical application. The boom layout was optimized to obtain the same minimal time lag response for the ten nozzles. The dynamic of the system was modeled using Simulink TM as first order model with delay. Two control strategies were implemented using PID (Proportional Integral Derivative) feedback control loops to monitor tracer injection (fluorescing) proportionally to simulated forward speed (from 0.6 to 1.2 m/s) and to control the constant operating pressure (constant carrier flow strategy) or the variable operating pressure proportionally to the injected chemical amount (variable total flow strategy). Different forward speed changes were induced using steps up and down, ramps, sine waves and sweeps excitations to evaluate the control feedback. The system stability was tested for its ability to maintain the expected concentration and application rate. The results show that the time lag remains less than 3 s (dead time < 2 s, time constant < 1 s) and the system keeps stable for the maximal speed variation (ΔV) and acceleration (ā) tested (ΔV = 200%, ā = 0.48 m/s2) which induce less than 10% variation of application rate. [less ▲]

Detailed reference viewed: 61 (21 ULg)
See detailDevelopment of products for GNSS users at the Belgian Solar-Terrestrial Centre of Excellence
Warnant, René ULg; Lejeune, Sandrine; Stankov, Stanimir et al

Conference (2008, December)

Detailed reference viewed: 117 (22 ULg)
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See detailDevelopment of PSII photochemistry after a single white flash in etiolated barley leaves
Franck, Fabrice ULg

in Baltscheffsky, M. (Ed.) Current Research in Photosynthesis (1990)

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See detailDevelopment of psoriasiform skin lesions under anti-TNF therapy: a genetic link?
Cleynen, I.; Claes, B.; Nuytten, H. et al

in Conference Abstract Book (2008)

Detailed reference viewed: 23 (3 ULg)
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See detailDevelopment of pure prolactin receptor antagonists
Bernichtein, Sophie; Kayser, Christine; Dillner, Karin et al

in Journal of Biological Chemistry (2003), 278(38), 35988-99

Prolactin (PRL) promotes tumor growth in various experimental models and leads to prostate hyperplasia and mammary neoplasia in PRL transgenic mice. Increasing experimental evidence argues for the ... [more ▼]

Prolactin (PRL) promotes tumor growth in various experimental models and leads to prostate hyperplasia and mammary neoplasia in PRL transgenic mice. Increasing experimental evidence argues for the involvement of autocrine PRL in this process. PRL receptor antagonists have been developed to counteract these undesired proliferative actions of PRL. However, all forms of PRL receptor antagonists obtained to date exhibit partial agonism, preventing their therapeutic use as full antagonists. In the present study, we describe the development of new human PRL antagonists devoid of agonistic properties and therefore able to act as pure antagonists. This was demonstrated using several in vitro bioassays, including highly sensitive assays able to detect extremely low levels of receptor activation. These new compounds also act as pure antagonists in vivo, as assessed by analyzing their ability to competitively inhibit PRL-triggered signaling cascades in various target tissues (liver, mammary gland, and prostate). Finally, by using transgenic mice expressing PRL specifically in the prostate, which exhibit constitutively activated signaling cascades paralleling hyperplasia, we show that these new PRL analogs are able to completely revert PRL-activated events. These second generation human PRL antagonists are good candidates to be used as inhibitors of growth-promoting actions of PRL. [less ▲]

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See detailDevelopment of Purkinje cells in the ovine brain
Salouci, Moustafa ULg; Engelen, Virginie; Gyan, Mathilda et al

in Anatomia, Histologia, Embryologia (2012), 41(3), 227232

Purkinje cells are involved in many vital functions within the body. Twenty ovine fetuses ranging from 2 to 5 months of gestation, two lambs in the first week after birth and three adult sheep were ... [more ▼]

Purkinje cells are involved in many vital functions within the body. Twenty ovine fetuses ranging from 2 to 5 months of gestation, two lambs in the first week after birth and three adult sheep were studied. Sections of the cerebellum were stained with haematoxylin and eosin, cresyl violet and Klu¨ver–Barrera. This study indicates that Purkinje cells began to appear after the 15th week of gestation. There were varying degrees of development of Purkinje cells in different zones of the cerebellum. Our findings in sheep fetuses suggest that the maturation of Purkinje cells starts in the caudal regions of the cerebellum and that the process begins in the vermis before it does in the cerebellar hemispheres. The alignment of Purkinje cells was found to be very regular in the caudal regions of the cerebellum. A partial absence of Purkinje cells in the rostral regions of the cerebellum was observed in both sheep fetuses and adult sheep. In the first post-natal week, some ectopic Purkinje cells were found in the white matter of the cerebellum. [less ▲]

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See detailDevelopment of Purkinje cells in the ovine brain
Salouci, Moustafa ULg; Engelen, Virginie; Gyan, Mathilda et al

Poster (2013, July 18)

Purkinje cells are involved in many vital functions within the body. Twenty ovine fetuses ranging from 2 to 5 months of gestation, two lambs in the first week after birth and three adult sheep were ... [more ▼]

Purkinje cells are involved in many vital functions within the body. Twenty ovine fetuses ranging from 2 to 5 months of gestation, two lambs in the first week after birth and three adult sheep were studied. Sections of the cerebellum were stained with haematoxylin and eosin, cresyl violet and Klu¨ver–Barrera. This study indicates that Purkinje cells began to appear after the 15th week of gestation. There were varying degrees of development of Purkinje cells in different zones of the cerebellum. Our findings in sheep fetuses suggest that the maturation of Purkinje cells starts in the caudal regions of the cerebellum and that the process begins in the vermis before it does in the cerebellar hemispheres. The alignment of Purkinje cells was found to be very regular in the caudal regions of the cerebellum. A partial absence of Purkinje cells in the rostral regions of the cerebellum was observed in both sheep fetuses and adult sheep. In the first post-natal week, some ectopic Purkinje cells were found in the white matter of the cerebellum. [less ▲]

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See detailDevelopment of Purkinje cells in the ovine brain
Salouci, Moustafa ULg; Engelen, Virginie; Gyan, Mathilda et al

Scientific conference (2012)

Detailed reference viewed: 20 (4 ULg)
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See detailDevelopment of quantification methods applied to HSS alloys for carbides volume fraction and grain size assessments
Tchuindjang, Jérôme Tchoufack ULg; Paydas, Hakan ULg; Hashemi, Seyedeh Neda ULg et al

Poster (2015, July)

High speed steels (HSS) are used in applications where enhanced mechanical properties together with hot oxidation and wear resistances are required. Such an improved physico-chemical behaviour is mainly ... [more ▼]

High speed steels (HSS) are used in applications where enhanced mechanical properties together with hot oxidation and wear resistances are required. Such an improved physico-chemical behaviour is mainly due to the presence of primary carbides within the microstructure. Depending of the initial chemical composition several types of carbides can precipitate in HSS such as MC (Nb and V-rich), M2C (Mo and W-rich), Cr-rich M7C3, etc. Two groups of HSS were analysed in this study, which are the so-called cast HSS grades that are manufactured by a centrifugal casting process and the cladded HSS manufactured by laser cladding. Purpose: Quantify the carbide volume fractions and the grain size within HSS grades in order to set correlations between the microstructure and both the mechanical and the tribological properties of the materials. Findings: The metallurgical features such as carbides nature and shape have been determined that are related to the manufacturing process of the studied material, and several methods have been used to quantify the carbide volume fractions and to assess the grain size. [less ▲]

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See detailThe development of radial glia and radial dendrites during barrel formation in mouse somatosensory cortex
Crandall, james E; Butler, darlene; Misson, Jean-Paul ULg

in Developmental Brain Research (1990), 55

Detailed reference viewed: 16 (1 ULg)
See detailDevelopment of real time PCR for quantification of Erwinia spp infecting potato tubers in Belgium
Moh, A.; Massart, Sébastien ULg; Roussel, S. et al

Poster (2006)

Detailed reference viewed: 17 (2 ULg)
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See detailDevelopment Of Real-Time Pcr For The Rapid Detection Of Episomal Banana Streak Virus (Bsv)
Delanoy, Martine; Salmon, Marc ULg; Kummert, Jean et al

in Plant Disease (2003), 87(1), 33-38

Detailed reference viewed: 32 (6 ULg)
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See detailDevelopment Of Real-Time Pcr Using Minor Groove Binding Probe To Monitor The Biological Control Agent Candida Oleophila (Strain O)
Massart, Sébastien ULg; De Clercq, Deborah; Dickburt, Catherine et al

in Journal of Microbiological Methods (2005), 60(1), 73-82

Detailed reference viewed: 18 (3 ULg)