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See detailDetection Of Apple Stem Grooving Virus In Dormant Apple Trees With Crude Extracts As Templates For One-Step Rt-Pcr
Marinho, Vla.; Kummert, J.; Rufflard, Gladys ULg et al

in Plant Disease (1998), 82(7),

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See detailDetection of Aspergillus spp. by PCR in Bronchoalveolar Lavage Fluid
Hayette, Marie-Pierre ULg; Vaira, Dolorès ULg; Suzin, Fabrice et al

in Journal of Clinical Microbiology (2001), 39(6), 2338-2340

The usefulness of a nested PCR assay for detection of Aspergillus sp. DNA was evaluated in 177 bronchoalveolar lavage (BAL) fluid specimens. This test was accurate both to diagnose culture-negative BAL ... [more ▼]

The usefulness of a nested PCR assay for detection of Aspergillus sp. DNA was evaluated in 177 bronchoalveolar lavage (BAL) fluid specimens. This test was accurate both to diagnose culture-negative BAL fluid specimens from patients with invasive pulmonary aspergillosis and to confirm culture-positive samples. However, it did not differentiate between infection and colonization. [less ▲]

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See detailDetection of Auroral Emissions from Callisto’s Magnetic Footprint at Jupiter
Clarke, J. T.; Wannawichian, S.; Hernandez, N. et al

Poster (2011, October)

HST observations of Jupiter’s aurora in a large campaign reveal several cases where the main oval emission appeared at unusually low latitudes, making it possible to search for the first time for auroral ... [more ▼]

HST observations of Jupiter’s aurora in a large campaign reveal several cases where the main oval emission appeared at unusually low latitudes, making it possible to search for the first time for auroral emissions from the magnetic footprint of Callisto without the overlapping bright emissions from the main oval. Several cases have been found where point-source emissions have now been detected from locations consistent with Callisto’s magnetic footprint on Jupiter at a brightness of ten’s of kilo- Rayleighs. These observations confirm that there is an electrodynamic interaction between Callisto and Jupiter’s magnetospheric environment that is similar to those at Io, Europa, and Ganymede, which all have auroral footprints. The properties of the emissions and a comparison with other observations and theoretical expectations will be presented in this paper. [less ▲]

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See detailDetection Of B-Cells And T-Cells, With Lectins Or Antibodies, In Healthy And Bovine Leukemia Virus-Infected Cattle
Fossum, C.; Burny, A.; Portetelle, Daniel ULg et al

in Veterinary Immunology and Immunopathology (1988), 18(3),

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See detailDetection of bacteria in sterile body fluids
CHRISTIAENS, Geneviève ULg; HAYETTE, Marie-Pierre ULg; De Mol, Patrick ULg

Poster (1998, October)

Purpose of the study: Development of a polymerase chain reaction (PCR) method for the detection of any bacterial DNA in synovial, cerebrospinal, pleural, and peritoneal fluids. Methods: Most of bacterial ... [more ▼]

Purpose of the study: Development of a polymerase chain reaction (PCR) method for the detection of any bacterial DNA in synovial, cerebrospinal, pleural, and peritoneal fluids. Methods: Most of bacterial species possess highly conserved, multicopy 16S ribosomal RNA genes, which can be hybridized with a single set of oligonucleotide primers. Samples were processed by an extraction protocol using proteinase K, and subjected to PCR amplification using two universal bacterial primers: RW01 and DG74; then the PCR products were detected by ethidium bromide gel electrophoresis. Study: Synovial, cerebrospinal, pleural and peritoneal fluids, obtained from 32 patients were analyzed by Gram stain, culture and PCR. Results: 1. The limit of detection, determined by analyzing successive dilutions of Staphylococcus aureus and Escherichia coli cultures in sterile water, was: 1.105 cfu/100µl. 2. We obtained 9 positive samples by culture: - 7 synovial fluids: S. agalactiae, S. viridans, coagulase negative Staphylococcus (2), S. epidermidis (2), and S. aureus. - 2 pleural fluids: S. pyogenes and Enterobacter aerogenes. All were PCR positive. 3. We tested 23 culture negative samples. All were negative by PCR. Conclusion: PCR presents some interesting features: 1. Only small amount of sample is necessary (100µl). 2. The duration of the test is shorter than 8 hours. 3. PCR provides similar or eventually greater sensitivity than culture and an excellent specificity (no false-positive and no false-negative results actually observed). [less ▲]

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See detailDetection of Banana Streak Virus (BSV) by IC-PCR-ELOSA
Delanoy, M.; Jijakli, Haissam ULg; Lepoivre, Philippe ULg

Conference (2001)

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See detailDetection of biomarkers of pathogenic bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Ruelle, Virginie; Elmoualij, Benaïssa ULg; Zorzi, Willy ULg et al

in Lichtfouse, Eric; Schwarzbauer, Jan; Robert, Didier (Eds.) Environmental Chemistry : Green Chemistry and Pollutants in Ecosystems (2005)

In recent years, various mass spectrometry procedures has been developed for identifying bacteria. The accuracy and speed with which data can be obtained by Matrix-assisted laser Desorption/Ionization ... [more ▼]

In recent years, various mass spectrometry procedures has been developed for identifying bacteria. The accuracy and speed with which data can be obtained by Matrix-assisted laser Desorption/Ionization Time-of-flight Mass Spectrometry (MALDI-TOF-MS) make this an advantageous technique for environmental monitoring. However, minor variations in the sample preparation can influence the mass spectra significantly. In the present study, we have introduced a procedure to prepare bacteria by microextraction and we have optimized experimental parameters for rapid identification by MALDI-TOF-MS of whole bacterial cells isolated from environmental samples such as wastewater and soil. [less ▲]

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See detailDetection of BLV in frozen semen samples by PCR assay
Dus Santos, M.J.; Rodriguez, Sabrina ULg; Wigdorovitz, A. et al

in AIDS Research and Human Retroviruses (2007, April 23), 23(4), 651

Detection of BLV in frozen semen samples by PCR assay Dus Santos Maria Jose; Rodriguez Sabrina; Wigdorovitz Andrés; Trono Karina Instituto de Virología, CICVyA, INTA-Castelar. CC 77. Buenos Aires ... [more ▼]

Detection of BLV in frozen semen samples by PCR assay Dus Santos Maria Jose; Rodriguez Sabrina; Wigdorovitz Andrés; Trono Karina Instituto de Virología, CICVyA, INTA-Castelar. CC 77. Buenos Aires, Argentina. Detection of BLV in frozen semen samples by PCR assay María José Dus Santos, Sabrina Rodriguez, Andrés Wigdorovitz and Karina Trono. Instituto de Virología, CICVyA, INTA-Castelar. CC 77. Buenos Aires, Argentina mdussantos@cnia.inta.gov.ar The sanitary and economic impact of BLV infection is associated with the interference in the international movement of cattle and their germ plasm. Although experimental data support the improbability that semen from BLV-positive bulls could infect recipient cows, restriction for commercialization of semen from infected animals is still present. The objective of this work was to standardize a PCR assay to diagnose the presence of BLV genome and to describe the pattern of BLV detection in frozen semen samples. The developed methodology involves the amplification of an internal fragment of gag gene and posses a limit of detection of 60 viral particles. Additionally, a biological test in susceptible sheep was performed in order to evaluate the transmission of BLV genome by semen from seropositive animals. This data strongly suggest that semen from seropositive bulls that resulted negative by PCR can be used for artificial insemination (AI), accompanied by proper collection protocols. Frozen semen samples from 30 seropositive bulls were analyzed. It was possible to detect proviral DNA in 118 out of 545 samples. It was important to note that BLV genome detection occurred in several collections but in an alternated way with non detection periods. On the other hand, in 4 seropositive bulls, it was not possible to detect BLV genome in all the samples analyzed. The development of this PCR assay constitutes a valuable diagnostic tool to determine the BLV-free status of semen used for AI. Moreover, the results suggest that BLV could present an intermittent pattern of excretion. [less ▲]

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See detailDetection of BLV in frozen semen samples by PCR assay.
Rossich, L.; Gutiérrez, G.; Rodriguez, Sabrina ULg et al

Poster (2007, November 12)

Intermitent detection of BLV in frozen semen simples by PCR assay L. Rossich1, J. Gutierrez1, S. Rodriguez1, E. Lefebvre2, K. Trono1 and M. J. Dus Santos1 (1) Instituto de Virología, CICVyA, INTA-Castelar ... [more ▼]

Intermitent detection of BLV in frozen semen simples by PCR assay L. Rossich1, J. Gutierrez1, S. Rodriguez1, E. Lefebvre2, K. Trono1 and M. J. Dus Santos1 (1) Instituto de Virología, CICVyA, INTA-Castelar. CC 77. Buenos Aires, Argentina. (2) Centro de Inseminación Artificial La Elisa. Argentina. Introduction: The major route of transmission of BLV is horizontal by direct exposure to biological fluids contaminated with infected lymphocytes, mainly blood. Although viral antigens and proviral DNA has been identified in semen, milk and colostrums, natural transmission through these secretions has not been demonstrated. The sanitary and economic impact of BLV infection is associated with the interference in the international movement of cattle and their germ plasm. Although experimental data support the improbability that semen from BLV-positive bulls could infect recipient cows, restriction for commercialization of semen from infected animals is still present[1-6]. Since there is no vaccine or treatment available, eradication and control of the disease is exclusively based on early diagnostic and segregation of infected animals. In this context the specificity and sensitivity of the diagnostic test used is a critical point. We have previously developed a PCR assay with high sensitivity and specificity to detect BLV genome in frozen semen samples. The objective of this work was to study the detection of BLV in semen. Material & methods: Samples: Serum, whole blood and semen samples were obtained from CIALE (Artificial Insemination Centre La Elisa, Capitan Sarmiento, Buenos Aires, Argentina) Serology: The agar gel immunodifussion (AGID) test kit used to detect antibodies to BLV was produced by the Faculty of Veterinary, La Plata University, Argentina. An indirect ELISA using recombinant p24 as antigen was used to detect antibodies to BLV. This assay has been completely developed and standardized in the Institute of Virology, INTA-Castelar, Buenos Aires, Argentina. Detection of proviral DNA: DNA was extracted from PMBCs (purified from whole blood) and semen samples (fresh and straw). PCR assays that amplified a region of genes pol and gag and a nPCR that amplified a region of gene env were developed. Results: Two PCR assays were standardized for detecting BLV genome in semen and PMBC. The limit of detection of viral particles was assessed by the addition of purified pBLV344 (a plasmid containing the complete BLV genome, kindly provided by Dr. Willems, Faculté Universitaire des Sciences Agronomiques, Gembloux, Belgium) to DNA from semen or PMBCs of a seronegative bull. By gag-PCR and pol-PCR, it was possible to detect 60 viral particles, using bromide staining after electrophoresis separation of DNA. In order to increase analytical sensitivity, a nested-PCR was developed which amplified a region of env gene. The n-PCR was able to detected 6 viral particles. Assessment of the limit of detection was highly repetitive under the standardized conditions. Frozen semen samples from 30 seropositive bulls were remitted to the laboratory and analyzed in the period 2005-2007. It was possible to detect proviral DNA in 172 out of 862 samples. BLV genome detection occurred in several collections but in an alternated way with non detection periods. Fresh semen samples, straws and whole blood were also obtained from 5 seronegative and 5 seropositive bulls and tested together for the presence of BLV provirus. Results indicated that while detection of provirus was positive in PMBC from all seropositive bulls, detection of gag, pol and env genes in semen did not occurred in all the samples. Discussions & conclusions: The results obtained suggest that BLV could present an intermittent pattern of excretion. Further studies should be done to confirmed the results obtained and to establish why the presence of BLV provirus in semen is not constant. References : 1. Choi, K, et al. 2002. Vet Diagn Invest 14: 403-406. 2. Kaja, R. and Olson, C. 1982. Theriogenology 18: 107-112. 3. Miller, J. and Van Der Maaten, M. 1979. J Natl Cancer Inst 62; 425-428. 4. Monke, D. 1986. JAVMA 188 (8): 823-826. 5. Romero, C., et al. 1983. Tropical Animal Health and production. 15: 215-218. 6. Straub O. 1982. In: Fourth International Symposium of Bovine Leukosis. The Hague: Martinus Nijhoff Publishers: 299-308. [less ▲]

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See detailDetection of bone sialoprotein in human (pre)neoplastic lesions of the uterine cervix
Detry, Cédric ULg; Waltregny, David ULg; Quatresooz, Pascale ULg et al

in Calcified Tissue International (2003), 73(1), 9-14

Bone sialoprotein (BSP) is a secreted glycoprotein primarily found in the mineral compartment of developing bones. BSP is detected in a variety of human cancers, particularly those that metastasize to the ... [more ▼]

Bone sialoprotein (BSP) is a secreted glycoprotein primarily found in the mineral compartment of developing bones. BSP is detected in a variety of human cancers, particularly those that metastasize to the skeleton. High expression of BSP in breast and prostate primary carcinomas is associated with progression and bone metastases development. Since squamous cell carcinoma (SCCs) of the uterine cervix also frequently metastasizes to bone, we investigated whether BSP is expressed in human cervical cancer. We examined BSP expression in cervical tissue samples from 47 patients, including 19 normal tissues, 20 squamous intraepithelial lesions (SILs) (9 low and 11 high grade) and 8 invasive SCCs. BSP protein expression was evaluated by the immunophosphatase technique using a BSP polyclonal antibody in paraffin-embedded cervical biopsies. The abundance of BSP protein was significantly higher in invasive SCCs and high grade SILs than in normal cervix tissue samples and low grade SILs, which showed no or a low level of anti-BSP immunoreactivity. In situ hybridization experiments performed on representative cervix invasive SCCs frozen sections revealed that BSP transcripts were detectable in these lesions. Our study demonstrates that BSP expression is a common feature in high grade SILs and invasive SCCs of the uterine cervix. The prognostic value of BSP detection in these lesions and the potential role of BSP as an angiogenic factor in this type of cancer are currently under investigation. [less ▲]

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See detailDetection of Bone Sialoprotein in Human Breast Cancer Tissue and Cell Lines at Both Protein and Messenger Ribonucleic Acid Levels
Bellahcene, Akeila ULg; Antoine, Nadine ULg; Clausse, N. et al

in Laboratory Investigation : Journal of Technical Methods & Pathology (1996), 75(2), 203-10

The recent demonstration that bone sialoprotein (BSP) can be detected in human breast cancer tissue by immunoperoxidase suggests that this phosphoprotein is ectopically expressed by malignant mammary ... [more ▼]

The recent demonstration that bone sialoprotein (BSP) can be detected in human breast cancer tissue by immunoperoxidase suggests that this phosphoprotein is ectopically expressed by malignant mammary epithelial cells. Its detection in human breast cancer cells raises questions about its potential role(s) during breast cancer progression. Because BSP is secreted and is present in the serum, the positivity of breast cancer cells for BSP could have been the result of an uptake of the circulating phosphoprotein by the cells rather than of an intrinsic expression. We examined the expression of BSP at both the protein and mRNA levels in nine human breast cancer samples as well as in three human breast cancer cell lines (MCF-7, T47-D, and MDA-MB-231) using immunohistochemistry, flow cytometric analysis, immunoblot, and reverse-transcriptase PCR. BSP was detected at both protein and mRNA levels in human breast cancer tissue and in the three human breast cancer cell lines. Using a specific polyclonal anti-BSP antibody, we showed by both fluorescence-activated cell sorter analysis and immunohistochemistry experiments that all of the human breast cancer cell lines studied express BSP. This was localized at the cell surface and in the cytosol of the estrogen receptor-positive MCF-7 and T47-D cell lines, whereas it was detected only in the cytosol of the estrogen receptor-negative MDA-MB-231 cells. Using the same polyclonal anti-BSP antibody, we were able to identify an approximately 97-kd band on total protein extracts from the three cell lines by immunoblotting. Reverse-transcriptase PCR reactions using specific oligonucleotides performed on total RNA of nine human breast cancer biopsy samples and the three cell lines demonstrated the presence of BSP mRNA in all of the samples examined. This study is the first demonstration that human malignant breast epithelial cell lines express BSP at the protein and mRNA levels. Our study identified MCF-7, T47-D, and MDA-MB-231 cells as useful models for the examination of the molecular mechanisms involved in the ectopic expression of BSP in breast malignant lesions. [less ▲]

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See detailDetection of bovine leukaemia virus antibodies. Use of monoclonal antibody to increase sensitivity in an ELISA test.
Portetelle, Daniel ULg; Bruck, C.; Mammerickx, M. et al

in Archives Internationales de Physiologie et de Biochimie (1982), 90(3),

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See detailDetection of Bovine Leukemia Virus antibodies : use of monoclonal antibody to increase sensitivity and specificity of the ELISA test.
Portetelle, Daniel ULg; Bruck, Claudine; Mammerickx, Marc et al

in Straub, O. C. (Ed.) Proceedings of the Fifth International Symposium on bovine leukosis (1984)

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See detailDetection of bovine leukemia virus specific antibodies using recombinant p24-ELISA.
Gutierrez, G.; Alvarez, I.; Fondevila, N. et al

in Veterinary Microbiology (2009), 137(3-4), 224-34

We developed an indirect ELISA test (rp24-ELISA) for the detection of antibodies against the p24 capsid protein of bovine leukemia virus (BLV) in bovine serum samples. A recombinant p24 (rp24) was ... [more ▼]

We developed an indirect ELISA test (rp24-ELISA) for the detection of antibodies against the p24 capsid protein of bovine leukemia virus (BLV) in bovine serum samples. A recombinant p24 (rp24) was expressed in the Escherichia coli expression system, purified in a nickel charged resin and used as antigen in the ELISA test. A cut-off point was established considering the distribution of reactivity values obtained by rp24-ELISA on a set of 555 field serum samples that were stated as double positive (DP) or double negative (DN) by the combination of commercial agar gel immunodiffusion (AGID) assay and gp51-ELISA tests results. The rp24-ELISA showed good concordance with the agar gel immunodiffusion assay, when 710 serum samples were analyzed. In addition, rp24-ELISA demonstrated to be a precise assay with good repeatability and reproducibility and a better analytical sensitivity than AGID. From 67 discordant rp24-ELISA-AGID sera, 4 negative reactors were from the DP group, 28 positive samples were from the DN group and 35 positive samples were stated as negative by AGID but positive by the gp51-ELISA. Samples from this last subgroup were sent to an OIE reference laboratory where 28 samples were stated as negative, 5 samples were stated as positive and 2 as inconclusive. Complete concordance with blind previous results from an international proficiency test panel confirmed the capability of the assay to discriminate between infected and non-infected animals. In conclusion, the rp24-ELISA developed and standardized demonstrated to have good analytical characteristics to be considered for screening of BLV. [less ▲]

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See detailDetection of bright multiply imaged quasars by GAIA
Finet, François ULg

Scientific conference (2011, June 06)

Bright multiply imaged quasars offer unique tools to investigate the properties of the Universe as a whole (cosmological parameters), the astrophysical properties of the remote galaxies acting as ... [more ▼]

Bright multiply imaged quasars offer unique tools to investigate the properties of the Universe as a whole (cosmological parameters), the astrophysical properties of the remote galaxies acting as deflectors (i.e. their mass, extinction law, ...) as well as the structure of the background sources (accretion disk, central engine, etc.). Multiply imaged quasars will represent the most easily identified signature of lensing in GAIA observations. Several thousands of such cases are expected with J <=20. Furthermore, the complete QSO catalog which will be built from the GAIA observations will be very attractive and unique in order to study QSO evolution, large scale structures, intervening absorbers, the inertial frame, ... The aim of this presentation is to report about a realistic estimate of the expected number of bright multiply imaged quasars that will be detected by GAIA. [less ▲]

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