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See detailThe detection and characterization of broad-leaved forest canopy gaps: a regeneration perspective
Bonnet, Stéphanie ULg; Bauwens, Sébastien; Lehaire, François ULg et al

Poster (2012, September)

Canopy gaps are areas of high regeneration potential and, in uneven-aged forest, gaps are therefore places of particular care for the forest manager. Nevertheless the cartography and characterization of ... [more ▼]

Canopy gaps are areas of high regeneration potential and, in uneven-aged forest, gaps are therefore places of particular care for the forest manager. Nevertheless the cartography and characterization of canopy gaps are complex issues. This paper addresses the fundamental question of the canopy gap definition: what is the minimal area, the maximal height of vegetation, type of regeneration, etc? From a regeneration point of view, canopy gaps can be defined as holes in the forest cover where light conditions are suitable for recruitment. As an active sensor, LiDAR has made it possible to tackle the problems of shadows and penetration into the canopy, typical of aerial images. This study investigates the cartography and characterization of forest canopy gaps as areas of natural regeneration. [less ▲]

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See detailDetection and Characterization of Extra-Solar Planets with Gaia
Lattanzi, M. G.; Casertano, S.; Jancart, Sylvie ULg et al

in O'Flaherty, K.S.; Perryman, M.A.C (Eds.) The Three-Dimensional Universe with Gaia (2005, January 01)

The scope of this paper is twofold. First, it describes the simulation scenario and the results of the large scale double-blind test campaign set-up by the Planetary System Working Group for the realistic ... [more ▼]

The scope of this paper is twofold. First, it describes the simulation scenario and the results of the large scale double-blind test campaign set-up by the Planetary System Working Group for the realistic simulation of the Gaia capabilities in detecting extra-solar planets. Present limitations and envisaged future improvements are also discussed. Then, the identified capabilities are put in context by highlighting the unique contributions that the Gaia exo-planet discoveries will be able to bring to the science of extra-solar systems of the next decade. [less ▲]

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See detailDetection and characterization of SNPs useful for identity control and parentage testing in major European dairy breeds
Werner, F. A. O.; Durstewitz, G.; Habermann, F. A. et al

in Animal Genetics (2004), 35(1), 44-49

We propose the use of single nucleotide polymorphisms (SNPs) instead of polymorphic microsatellite markers for individual identification and parentage control in cattle. To this end, we present an initial ... [more ▼]

We propose the use of single nucleotide polymorphisms (SNPs) instead of polymorphic microsatellite markers for individual identification and parentage control in cattle. To this end, we present an initial set of 37 SNP markers together with a gender-specific SNP for identity control and parentage testing in the Holstein, Fleckvieh and Braunvieh breeds. To obtain suitable SNPs, a total of 91.13 kb of random genomic DNA was screened yielding 531 SNPs. These, and 43 previously identified SNPs, were subjected to the following selection criteria: (1) the frequency of the minor allele must be larger than 0.1 in at least two of the three examined breeds, and (2) markers should not be linked closely. Allele frequencies were estimated by analysing sequencing traces of pooled DNA or by genotyping individual DNA samples. The selected SNP loci were physically mapped by radiation hybrid mapping or by fluorescence in situ hybridization, and tested against the neutral mutation hypothesis. The presented marker set theoretically allows probabilities of identity less than 10(-13) for individual verification and exclusion powers exceeding 99.99% for parentage testing. [less ▲]

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See detailDetection and characterization of tilted peptides in amyloid proteins
Crowet, Jean-Marc ULg; Lins, Laurence ULg; Brasseur, Robert ULg

Poster (2009, April 25)

The study of amyloidogenic proteins is of interest in biochemistry because these proteins undergo conformational changes and aggregation. Both processes are largely implicated in several diseases ... [more ▼]

The study of amyloidogenic proteins is of interest in biochemistry because these proteins undergo conformational changes and aggregation. Both processes are largely implicated in several diseases including Alzheimer’s, Parkinson’s or Creutzfeldt-Jakob’s disease. These phenomena are not completely understood, either at a structural or energetical point of view. Tilted peptides are short protein fragment (11 to 19 residues) that adopt a tilted orientation when inserted into biological membranes and destabilise them. Recently, tilted peptides have been detected in two amyloidogenic proteins involved in neurodegenerative diseases; the amyloid  peptide responsible for Alzheimer’s disease, and the PrP protein that causes Creutzfeldt-Jakob’s disease. Tilted peptides could be responsible for the neurotoxic effects of these proteins. Due to their destabilising properties, they could interact directly with the membrane leading to cell death. Tilted peptides could also be involved in the transconformational process of the proteins. The aim of this work is to detect tilted fragments in other amyloidogenic proteins by molecular modelling and to study some of these peptides experimentally to evidence their lipid destabilizing properties, their structure and their toxicity. Twenty-four tilted peptides from 18 different proteins have been detected among 53 amyloidogenic proteins and 7 peptides were tested experimentally. The results support the hypothesis that some tilted peptides could be involved in transconformational processes and/or cytotoxicity related to amyloidogenic proteins. [less ▲]

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See detailDetection and characterization of Vibrio parahaemolyticus in seafood using real time PCR.
Bradatan G; China B; Daube, Georges ULg

Poster (2005, June)

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See detailDetection and characterization of VIM-31, a new variant of VIM-2 with Tyr224His and His252Arg mutations, in a clinical isolate of Enterobacter cloacae.
Bogaerts, Pierre; Bebrone, Carine ULg; Huang, Te-Ding et al

in Antimicrobial Agents and Chemotherapy (2012), 56(6)

We report the first description of the metallo-β-lactamase VIM-31, a new variant of VIM-2 with Tyr224His and His252Arg mutations, in Enterobacter cloacae 11236, which was isolated from blood specimens of ... [more ▼]

We report the first description of the metallo-β-lactamase VIM-31, a new variant of VIM-2 with Tyr224His and His252Arg mutations, in Enterobacter cloacae 11236, which was isolated from blood specimens of a patient with colonic adenocarcinoma in Belgium. bla(VIM-31) was found on a class 1 integron located on a self-transferable but not typeable 42-kb plasmid. Compared to values published elsewhere for VIM-2, the purified VIM-31 enzyme showed weaker catalytic efficiency against all the tested beta-lactam agents (except for ertapenem), resulting from lower k(cat) (except for ertapenem) and higher K(m) values for VIM-31. [less ▲]

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See detailDetection and clinical usefulness of a biphasic response during exercise echocardiography early after myocardial infarction
Lancellotti, Patrizio ULg; Hoffer, E. P.; Pierard, L. A.

in Journal of the American College of Cardiology (2003), 41(7), 1142-1147

OBJECTIVES The aim of this study was to determine the accuracy of exercise echocardiography (EE) for detecting infarct-related artery (IRA) stenosis and predicting functional recovery early after acute ... [more ▼]

OBJECTIVES The aim of this study was to determine the accuracy of exercise echocardiography (EE) for detecting infarct-related artery (IRA) stenosis and predicting functional recovery early after acute myocardial infarction (AMI). BACKGROUND Dobutamine stress echocardiography is widely used for identifying jeopardized myocardium. The clinical usefulness of a biphasic response detected during EE has never been investigated. METHODS A total of 114 consecutive patients with a first AMI and greater than or equal to2 dyssynergic segments in the infarct-related territory underwent semi-supine continuous EE 6 +/- 2 days after AMI. Quantitative coronary angiography was performed in all patients after EE. A follow-up echocardiogram was obtained one month later. RESULTS Ninety-seven patients had significant (greater than or equal to50%) IRA stenosis, and 26 had multivessel disease. Residual ischemia was identified in 77 patients (biphasic response in 62 and worsening response in 15). The sensitivity and specificity of ischemia during EE for predicting IRA stenosis were 75% and 76%, respectively. The sensitivity of a biphasic response was higher than the sensitivity of a worsening response (61% vs. 14%, p < 0.0001). Wall motion abnormalities induced in other vascular territories were specific (97%) and moderately sensitive (62%) for the detection of multivessel disease. Functional recovery was observed in 75 patients. Two independent variables predicted contractile recovery: contractile reserve during EE (p < 0.0001) and elective angioplasty of the IRA (p = 0.002). A biphasic response, but not sustained improvement, predicted reversible dysfunction (73% vs. 9%, p < 0.0001). CONCLUSIONS A biphasic response can be detected during exercise. Exercise echocardiography is an accurate tool for detecting IRA stenosis and predicting functional improvement early after AMI. [less ▲]

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See detailDetection and Correction of Outliers for Fatty Acids Contents Measured by Mid-Infrared Spectrometry Using Random Regression Test-Day Models
Soyeurt, Hélène ULg; Dardenne, Pierre; Gengler, Nicolas ULg

in Book of Abstracts of the 60th Annual Meeting of the European Association for Animal Production (2009)

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See detailDetection and epidemiological characteristics of peach latent mosaic viroid in Tunisia.
Hassen, I. F.; Roussel, S.; Kummert, J. et al

in Communications in Agricultural and Applied Biological Sciences (2005), 70(3), 115-128

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See detailDetection and identification of hydrophilic selenium compounds in selenium-rich yeast by size exclusion-microbore normal-phase HPLC with the on-line ICP-MS and electrospray Q-TOF-MS detection
Far, Johann ULg; Preud'homme, Hugues; Lobinski, Ryszard

in Analytica Chimica Acta (2010), 657(2),

Normal-phase HPLC and hydrophilic interaction HPLC (HILIC) were investigated for the separation of selenometabolites in a water extract of Se-rich yeast prior to their detection by ICP-MS and ... [more ▼]

Normal-phase HPLC and hydrophilic interaction HPLC (HILIC) were investigated for the separation of selenometabolites in a water extract of Se-rich yeast prior to their detection by ICP-MS and identification by electrospray MS/MS. The targeted fraction was a low-abundant fraction co-eluting with salt and sulfur analogues in size-exclusion chromatography which has so far been inaccessible to Se speciation studies. The optimization of the separation conditions resulted in the highest separation efficiency when HILIC was used and elution was carried out isocratically with a low concentration ammonium acetate buffer (1 mM ammonium acetate/10mM acetic acid) in 80% acetonitrile. Out of 15 peaks observed with the Se-specific ICP-MS detection 12 was identified by electrospray Q-TOF MS/MS (2,3-dihydroxypropionyl (DHP)-Se-methylselenocysteine [M+H](+): 272, Se-methyl-gamma-glutamyl-selenocysteinylglycine dioxide [M+H](+): 402, gamma-glutanlyl-Se-methylselenocysteine[M+H](+): 313; isomers of gamma-glutamylselenocystathionine [M+H](+): 400; Se-methyl-selenoglutathione [M+H](+): 370, isomers of N-acetylselenocystathionine [M+H](+): 313, 2,3-DHP-selenohomolanthionine [M+H](+): 373, isomers of 2,3-DHP-seienocystathionine[M+H](+): 359,2,3-DHP-selenolanthionine [M+H](+): 345 and selenohomolanthionine [M+H](+): 285). (C) 2009 Elsevier B.V. All rights reserved. [less ▲]

Detailed reference viewed: 29 (6 ULg)
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See detailDetection and Identification of Pathotypes of Verocytotoxigenic Escherichia Coli Isolated from Weaned Piglets Using Gene Probes for Seven E. Coli Toxins
Mainil, Jacques ULg; Daube, Georges ULg; Deprez, Piet et al

in FEMS Microbiology Letters (1989), 50(3), 345-349

Seventy verocytotoxigenic (VTEC) and sixty-three non VTEC haemolytic Escherichia coli isolated from recently weaned piglets were examined by the colony hybridization assay using gene probes for three ... [more ▼]

Seventy verocytotoxigenic (VTEC) and sixty-three non VTEC haemolytic Escherichia coli isolated from recently weaned piglets were examined by the colony hybridization assay using gene probes for three verocytotoxins: Edema disease principle (EDP) and Shiga-like toxins I and II (SLTI and SLTII). The results with the EDP and SLTII probes were identical. All VTEC hybridized with these two probes, while non VTEC did not. All 133 E. coli were negative for the SLTI probe. Hybridization of the plasmid content of 14 VTEC did not show any evidence for plasmid localization of the genes coding for the EDP. The 70 VTEC were also assayed with gene probes for heat-stable (STaP, STb) and heat-labile (LT, LTIIa) enterotoxins. Only the STb probe was hybridized by 36 of them. Most STb-positive isolates belonged to serotype O141: K85 biotypes 9 and 13 PC. [less ▲]

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See detailDetection And Identification Of Sweet Potato Viruses By The Polymerase Chain Reaction
Colinet, D.; Lepoivre, Philippe ULg; Xia, Fz. et al

in Agro Food Industry Hi-Tech (1996), 7(2),

Detailed reference viewed: 11 (0 ULg)
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See detailDetection and identification of the cytomegalovirus genome in infected cells with labeled DNA probes
Bourdon-Wouters, Christine; Merville, Marie-Paule ULg; Sadzot-Delvaux, Catherine ULg et al

in Archives Internationales de Physiologie et de Biochimie (1987)

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See detailDetection and Localisation of Damage on Industrially Produced Concrete Slabs Through Time- and Frequency-Domain Approaches
Nguyen, Viet Ha; Mahowald, Jean; Golinval, Jean-Claude ULg et al

in Carrera, E; Miglioretti, F; Petrolo, M (Eds.) 6th ECCOMAS Thematic Conference on Smart Structures and Materials (SMART2013) (2013, June)

The objective of this work is to address the problem of damage detection in civil engineering structures using non-destructive techniques and dynamic measurements. To this purpose, time- or frequency ... [more ▼]

The objective of this work is to address the problem of damage detection in civil engineering structures using non-destructive techniques and dynamic measurements. To this purpose, time- or frequency-domain methods are used for the diagnostics. It consists in practical output-only techniques as Stochastic Subspace Identification (SSI) for modal identification or Enhanced Principal Component Analysis (EPCA) for detecting the presence of damage. The use of the Hankel matrix instead of the observation matrix improves effectively the robustness of these methods. Damage localization is based on Frequency Response Functions (FRFs) and sensitivity analysis of PCA results. The efficiency of the above-mentioned methods has been demonstrated in earlier studies mainly on numerical models and small-scale laboratory experiments [3, 4]. It was also tested successfully on industrial examples to perform machine condition monitoring using a reduced set of sensors [2]. In this work, the investigation is performed on precast prestressed and non-prestressed concrete slabs. Successive damages were artificially introduced in the slabs by loading heavy weights and by cutting steel wires, which induced cracks in the structure. The examples show the consequences of the considered techniques for damage identification. The results that are very different between prestressed and non-prestressed slabs may be used as input for the condition control of this kind of structures. [less ▲]

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See detailDetection and modelling of the shear band localisation
Charlier, Robert ULg; Pierry, Joelle

in Proc. of the Int. Conf. on Computational Plasticity, Fundamentals and Applications, COMPLAS (1995, April)

Detailed reference viewed: 11 (3 ULg)