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See detailDeveloping tools for managing hydrogeological data in a semi-arid region: the case study of Oulmès (Morocco)
Ruthy, Ingrid ULg; Orban, Philippe ULg; Elbatloussi, D. et al

in GIRE3D: International Congress on Integrated Water Resources management and Challenges of the Sustainable Development (2006)

Data and information required by hydrogeological studies are various and complex such as geology, topography, climate, hydrological and hydrogeological raw or pre-processed data. All these data need to be ... [more ▼]

Data and information required by hydrogeological studies are various and complex such as geology, topography, climate, hydrological and hydrogeological raw or pre-processed data. All these data need to be managed, and this can be done in a structured database. A (GIS-managed) hydrogeological database has been developed previously by the Hydrogeology Group of the University of Liège (Gogu et al., 2001) in order to capture, store, edit, query, update and display geographically referenced hydrogeological data. The development of this geo-relational database was constrained by a maximum storage of data with a minimum data redundancy, reduction of storage memory capacity and optimum ‘retrievability’ of information for further analysis. In recent years, the use of Geographical Information System (GIS) has grown rapidly in groundwater management and research. GIS is now widely used to create digital geographic databases, to manipulate and prepare data as input for various model parameters, and to display model output. Coupling GIS with this hydrogeological database provides a powerful tool. This GIS-managed hydrogeological database has been also developed for vulnerability-assessment techniques and numerical modelling for groundwater flow and contaminant transport studies. As well as linking between database and GIS, the design of coupling database with process-based numerical models was also performed. This methodology has been applied, in a semi-arid region: the "Oulmès plateau" located in the Mid-Atlas (Morocco). This study has led to a detailed hydrogeological map at the scale of 1/25000. First, data were collected from existing databases, studies and maps as well as through new field measurements. These data came from numerous and various sources and in different formats (paper or digital diagrams, images, spreadsheets…). After validation, it has been encoded in the hydrogeological database. Because data are geo-referenced, it can be easily represented on the map within GIS-software. The hydrogeological map displays several layers of information as topography, hydrogeological units, hydrographic network, wells, piezometers, isoline of piezometric heads. [less ▲]

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See detailDeveloping Tourism Products and new Partnerships through Participative Action Research in Rural Cameroon
Schmitz, Serge ULg; Lekane Tsoubgou, Dieudonné

in Geographical Research (2016), 54(2), 143-152

The paper explores possible synergies between microfinance institutions and small and medium medium tourism businesses in the West Cameron Chiefdooms. Based on a research action the authors emphasise the ... [more ▼]

The paper explores possible synergies between microfinance institutions and small and medium medium tourism businesses in the West Cameron Chiefdooms. Based on a research action the authors emphasise the obstacles to the formation of partnership and describe how the researchers facilitated networking. [less ▲]

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See detailDevelopmednt of an integrated modelling methodology for compartment fires by implementing a weak coupling strategy between a CFD and an FE software
Tondini, Nicola; Franssen, Jean-Marc ULg

in De Luca, Antonello (Ed.) XXIII giornate italiane della costruzione in acciaio (2011, October 12)

This article presents the assumptions and the issues that arise when developing an integrated modelling methodology between a Computational Fluid Dynamics (CFD) software applied to compartment fires and a ... [more ▼]

This article presents the assumptions and the issues that arise when developing an integrated modelling methodology between a Computational Fluid Dynamics (CFD) software applied to compartment fires and a finite element (FE) software applied to structural systems. In particular, a weak coupling methodology use to simulate a fire exposed structure by modelling the fire development in the compartment, the heat penetration in the structure and the mechanical response is described. The advantages and the disadvantages of such a technique are highlighted compared to a full coupling that conversely takes into account all mutual interactions. This methodology is particularly suitable for relatively thin steel structures in large compartments. Moreover, the need of a standardised transfer file in order to get the methodology as flexible as possible in term of versatility is emphasized. [less ▲]

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See detailDevelopment and aging of the Kisspeptin-GPR54 system in the mammalian brain: what are the impacts on female reproductive function?
Franceschini, Isabelle; Desroziers, Elodie ULg

in Frontiers in Neuroendocrine Science (2013)

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See detailDevelopment and application of a microplate method to evaluate the efficacy of essential oils against Penicillium italicum Wehmer, Penicillium digitatum Sacc. and Colletotrichum musea (Berk. M.A. Curtis) Arx, three postharvest fungal pathogens of fruits
Kouassi, Kouadio Hugues Sosthène ULg; Bajji, M.; Brostaux, Yves ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2012), 16(3), 325-336

A microbioassay was developed for evaluating the in vitro antifungal activity of 30 preselected essential oils. A template based on 10 serial dilutions with eight replicates per dilution arranged on two ... [more ▼]

A microbioassay was developed for evaluating the in vitro antifungal activity of 30 preselected essential oils. A template based on 10 serial dilutions with eight replicates per dilution arranged on two 96-well ELISA plates was used as a reproducible and standardized design to identify the in vitro effectiveness of these essential oils against Penicillium italicum Wehmer, Penicillium digitatum Sacc. and Colletotrichum musea (Berk. M.A. Curtis) Arx, three postharvest fungal pathogens, on fruits. Growth of mycelium was monitored by measuring optical density (492 nm). Cinnamomum zeylanicum, Cinnamomum verum and Eugenia caryophyllus were found to be still active against all the three pathogens even at 100 ppm. Compared to other methods, this microbioassay proved to be a rapid, reproducible, and efficient method for testing the efficacy of essential oils that inhibit spore germination in P. italicum, P. digitatum and C. musea. The assay requires relatively small amounts of essential oils. [less ▲]

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See detailDevelopment and application of a non-targeted extraction method for the analysis of migrating compounds from plastic baby bottles by GC-MS
Onghena, Matthias; van Hoeck, Els; Vervliet, Philippe et al

in Food Additives & Contaminants. Part A. Chemistry, Analysis, Control, Exposure & Risk Assessment (2014), 31(12), 2090-2102

In 2011, the European Union prohibited the production of polycarbonate (PC) baby bottles due to the toxic effects of the PC monomer bisphenol-A. Therefore, baby bottles made of alternative materials, e.g ... [more ▼]

In 2011, the European Union prohibited the production of polycarbonate (PC) baby bottles due to the toxic effects of the PC monomer bisphenol-A. Therefore, baby bottles made of alternative materials, e.g. polypropylene (PP) or polyethersulphone (PES), are currently marketed. The principal aim of the study was the identification of major compounds migrating from baby bottles using a liquid–liquid extraction followed by GC/MS analysis. A 50% EtOH in water solution was selected as a simulant for milk. After sterilisation of the bottle, three migration experiments were performed during 2 h at 70°C. A nontargeted liquid–liquid extraction with ethyl acetate–n-hexane (1:1) was performed on the simulant samples. Identification of migrants from 24 baby bottles was done using commercially available WILEY and NIST mass spectra libraries. Differences in the migrating compounds and their intensities were observed between the different types of plastics, but also between the same polymer from a different producer. Differences in the migration patterns were perceived as well between the sterilisation and the migrations and within the different migrations. Silicone, Tritan™ and PP exhibited a wide variety of migrating compounds, whereas PES and polyamide (PA) showed a lower amount of migrants, though sometimes in relatively large concentrations (azacyclotridecan-2-one up to 250 µg kg−1 ). Alkanes (especially in PP bottles), phthalates (dibutylphthalate in one PP bottle (±40 µg kg−1 ) and one silicone bottle (±25 µg kg−1 ); diisobutylphthalate in one PP (±10 µg kg−1 ), silicone (up to ±80 µg kg−1 ); and Tritan™ bottle (±30 µg kg−1 )), antioxidants (Irgafos 168, degradation products of Irganox 1010 and Irganox 1076), etc. were detected for PP, silicone and Tritan™ bottles. Although the concentrations were relatively low, some compounds not authorised by European Union Regulation No. 10/2011, such as 2,4-di-tert-butylphenol (10–100 µg kg−1 ) or 2-butoxyethyl acetate (about 300 µg kg−1 ) were detected. Migrating chemicals were identified as confirmed (using a standard) or as tentative (further confirmation required). [less ▲]

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See detailDevelopment And Application Of A Scar Marker To Monitor And Quantify Populations Of The Postharvest Biocontrol Agent Pantoea Agglomerans Cpa-2
Nunes, C.; Bajji, M.; Stepien, V. et al

in Postharvest Biology & Technology (2008), 47(3), 422-428

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See detailDevelopment and application of a SYBR Green RT-PCR for first line screening and quantification of porcine sapovirus infection
Mauroy, Axel ULg; Van der Poel, W.H.M.; Hakze-Van der Honing, R. et al

in BMC Veterinary Research (2012), 8

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See detailDevelopment and application of high-resolution petrography on resin-impregnated Holocene peat columns to detect and analyse tephras, cryptotephras, and other materials
De Vleeschouwer, François ULg; van Vliet-Lanoe, Brigitte; Fagel, Nathalie ULg et al

in Quaternary International (2008), 178

We describe the potential for high-resolution detection, observation and chemical analysis of tephras and cryptotephras in freeze-dried and resin-impregnated peat sections. Special attention is drawn to ... [more ▼]

We describe the potential for high-resolution detection, observation and chemical analysis of tephras and cryptotephras in freeze-dried and resin-impregnated peat sections. Special attention is drawn to tephra grain alteration products, sensitive to standard lab treatment, but preserved using an impregnation technique developed here. Resulting blocks and thin sections provide a continuous archive of tephras that can be investigated through a wide range of non-destructive techniques encompassing polarising and fluorescence microscopy, scanning electron microscopy with/without elemental analysis, and XRF-based core scanning. (c) 2007 Elsevier Ltd and INQUA. All rights reserved. [less ▲]

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See detailDevelopment and application of multiple internal reference (housekeeper) gene assays for accurate normalisation of canine gene expression studies
Peters, I. R.; Peeters, Dominique ULg; Helps, C. R. et al

in Veterinary Immunology and Immunopathology (2007), 117(1-2), 55-66

Measurement of mRNA expression by real-time RT-PCR (QRT-PCR) has proven to be an important and powerful tool for the investigation of the pathogenesis of inflammatory and immune-mediated diseases in many ... [more ▼]

Measurement of mRNA expression by real-time RT-PCR (QRT-PCR) has proven to be an important and powerful tool for the investigation of the pathogenesis of inflammatory and immune-mediated diseases in many species. This methodology has proven particularly valuable in the dog, a species for which there are currently few specific antibodies for measurement of relevant proteins. Internal control (housekeeper) mRNAs are widely used for normalisation of QRT-PCR results. The validation and use of multiple internal control mRNAs for increased accuracy of normalisation has been described for humans and rodents. The aims of this study were to develop QRT-PCR assays for 11 potential internal control mRNAs in the dog (ACTB, B2M, G3PDH, HMBS, HPRT1, RPL 13A, RPL32, RPS 18, SDHA, TBP and YWAZ) and validate their use with bone marrow, colon, duodenum, heart, kidney, liver, lung, lymph node, skeletal muscle, pancreas, spleen and stomach from seven dogs. Endoscopic biopsies of the superficial duodenal mucosa were also obtained from nine dogs suffering from chronic gastro-oesophageal disease. The most stably expressed genes varied in the tissues examined. RPL13A and RPL32 (both components of the 60S ribosomal subunit) were the most stably expressed genes in the majority of the tissues examined, whereas ACTB and B2M were the least stable. Distinct internal control genes were shown to be most appropriate for use in full-thickness versus superficial mucosal biopsies of the duodenum. The results of this study indicate that there are no universal control genes for gene expression studies in canine tissues. It is important to use multiple internal control genes based upon a survey of potential control genes applied to representative samples from different disease groups, culture conditions and/or time points in an experimental study. (C) 2007 Elsevier B.V. All rights reserved. [less ▲]

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See detailDevelopment and application of test cases for comparing vertical ground heat exchangers models
Bernier, Michel; Kummert, Michael; Bertagnolio, Stéphane ULg

in Proceedings of the 10th International IBPSA Conference, Beijing, China (2007, September)

The main objective of this paper is to establish a set of test cases for analytical verifications and intermodel comparisons of ground heat exchanger (GHX) models used in building simulation programs ... [more ▼]

The main objective of this paper is to establish a set of test cases for analytical verifications and intermodel comparisons of ground heat exchanger (GHX) models used in building simulation programs. Several test cases are suggested. They range from steady-state heat rejection in a single borehole to varying hourly loads with large yearly thermal imbalance in multiple borehole configurations. The usefulness of the proposed test cases is illustrated by running them with different GHX models. This comparison exercise has shown that 1-D models compare favourably well with the more elaborate 3-D models for relatively small simulation periods. The cyclic heat rejection/collection test has revealed some small deficiency in the load aggregation scheme of a particular model. Finally, the use of the asymmetric (cooling dominated) load profile test case for a bore field composed of 100 boreholes revealed that the borehole wall temperature predicted by two GHX models can differ by as much as 10oC after a 30 year simulation. [less ▲]

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See detailThe development and applications of a ground-based fiber nulling coronagraph
Martin, Stefan; Serabyn, Eugene; Liewer, Kurt et al

in Optical and Infrared Interferometry (2008, July)

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See detailDevelopment and assessment of the Osteoporosis Index of Risk (OSIRIS) to facilitate selection of women for bone densitometry
Sedrine, W. B.; Chevallier, T.; Zegels, Brigitte ULg et al

in Gynecological Endocrinology : The Official Journal of the International Society of Gynecological Endocrinology (2002), 16(3), 245-250

A simple questionnaire would be useful to identify individuals most in need of bone mineral density (BMD) testing. We designed a new predictive model and risk assessment instrument based on an extensive ... [more ▼]

A simple questionnaire would be useful to identify individuals most in need of bone mineral density (BMD) testing. We designed a new predictive model and risk assessment instrument based on an extensive review of the literature evaluating risk factors for osteoporosis, and tested its performance in a large cohort of postmenopausal women in whom BMD was measured by dual x-ray absorptiometry. In total, 1303 postmenopausal women from an outpatient osteoporosis clinic participated in this study. The Osteoporosis Index of Risk (OSIRIS) is based on four variables: age, body weight, current hormone replacement therapy use and history of previous low impact fracture. The sensitivity and specificity for an OSIRIS value of +1 were respectively 78.5% and 51.4%. The AUC under the ROC curve of OSIRIS was 0.71. Three categories were arbitrarily created using OSIRIS, with cutoff of +1 and -3. The low risk category (OSIRIS > +1) represented 41% of all women; only 7% of the women in this category had osteoporosis. The prevalence of osteoporosis was very high (66%) among the group at high risk (OSIRIS < -3 representing 15% of all women). The prevalence of osteoporosis was 39% in the intermediate risk group (-3 < OSIRIS < +1, 44% of all women). In conclusion, OSIRIS is a simple index based on four easy-to-collect variables from postmenopausal women, it shows a high degree of accuracy, and performed well for classifying the degree of risk of osteoporosis in western European women of Caucasian lineage. Based on this instrument it is possible to propose a strategy that would initiate treatment in women with very high risk, postpone BMD measurement in women with low risk and limit BMD measurement to women with intermediate risk of osteoporosis, this would spare more than 55% of the densitometry bill compared with a mass screening scenario. [less ▲]

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See detailDevelopment and characterization of microsatellite loci in Pericopsis elata (Fabaceae) using a cost-efficient approach
Micheneau, Claire; Dauby, Gilles; Bourland, Nils ULg et al

in American Journal of Botany (2011)

Premise of the study : Microsatellite loci were developed in the endangered Pericopsis elata using a combination of low-cost procedures. Methods and Results : Microsatellite isolation was performed ... [more ▼]

Premise of the study : Microsatellite loci were developed in the endangered Pericopsis elata using a combination of low-cost procedures. Methods and Results : Microsatellite isolation was performed simultaneously on three distinct species through a newly available procedure that associates multiplex microsatellite enrichment and next-generation sequencing, allowing the rapid and low-cost development of microsatellite-enriched libraries through the use of a 1/32nd GS-FLX plate. Genotyping using M13-like label- ing in multiplexed reactions allowed additional cost savings. From 72 primers selected for initial screening, 21 positively amplified P. elata , and 11 showed polymorphism with two to 11 alleles per locus and a mean value of 5.4 alleles per locus. Conclusions : These microsatellite loci will be useful to further investigate the level of genetic variation within and between natural populations of P. elata in Africa. [less ▲]

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See detailDevelopment and characterization of SSR markers for pomegranate (Punica granatum L.) using an enriched library
Hasnaoui, Nejib ULg; Buonamici, Anna; Sebastiani, Federico et al

in Conservation Genetics Resources (2010), 2

In the present work, we report the development of 11 microstallite markers (SSR) for Punica granatum. Evaluated on a set of 27 pomegranate accessions sampled in Tunisia, they displayed 25 alleles, with ... [more ▼]

In the present work, we report the development of 11 microstallite markers (SSR) for Punica granatum. Evaluated on a set of 27 pomegranate accessions sampled in Tunisia, they displayed 25 alleles, with number of alleles per locus ranging between 1 and 4, and an observed heterozygosity from 0.037 and 0.592. This set of SSR markers can be very useful for studies dealing with genetic diversity assessment of germplasm, with cultivars/varieties fingerprinting and pedigree analysis of this economically important fruit species. [less ▲]

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See detailDevelopment and epidemiological evaluation of a monoclonal ELISA detecting Bovine Viral Diarrhœa Pestivirus antigens in field blood samples of persistently infected cattle
Mignon, Bernard ULg; Dubuisson, J.; Waxweiler, S. et al

in Steven, Edwards (Ed.) ESVV-Proceedings of the second symposium on pestiviruses (1993)

Detailed reference viewed: 10 (7 ULg)