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See detailDevelopment of a self-administrated quality of life questionnaire for sarcopenia in elderly subjects: the SarQol
Beaudart, Charlotte ULg; Biver, Emmanuel; Reginster, Jean-Yves ULg et al

in Age & Ageing (2015), 44

BACKGROUND: the impact of sarcopenia on quality of life is currently assessed by generic tools. However, these tools may not detect subtle effects of this specific condition on quality of life. OBJECTIVE ... [more ▼]

BACKGROUND: the impact of sarcopenia on quality of life is currently assessed by generic tools. However, these tools may not detect subtle effects of this specific condition on quality of life. OBJECTIVE: the aim of this study was to develop a sarcopenia-specific quality of life questionnaire (SarQoL, Sarcopenia Quality of Life) designed for community-dwelling elderly subjects aged 65 years and older. SETTINGS: participants were recruited in an outpatient clinic in Liège, Belgium. SUBJECTS: sarcopenic subjects aged 65 years or older. METHODS: the study was articulated in the following four stages: (i) Item generation-based on literature review, sarcopenic subjects' opinion, experts' opinion, focus groups; (ii) Item reduction-based on sarcopenic subjects' and experts' preferences; (iii) Questionnaire generation-developed during an expert meeting; (iv) Pretest of the questionnaire-based on sarcopenic subjects' opinion. RESULTS: the final version of the questionnaire consists of 55 items translated into 22 questions rated on a 4-point Likert scale. These items are organised into seven domains of dysfunction: Physical and mental health, Locomotion, Body composition, Functionality, Activities of daily living, Leisure activities and Fears. In view of the pretest, the SarQoL is easy to complete, independently, in ∼10 min. CONCLUSIONS: the first version of the SarQoL, a specific quality of life questionnaire for sarcopenic subjects, has been developed and has been shown to be comprehensible by the target population. Investigations are now required to test the psychometric properties (internal consistency, test-retest reliability, divergent and convergent validity, discriminant validity, floor and ceiling effects) of this questionnaire. [less ▲]

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See detailDevelopment of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21)
Nollevaux, Géraldine; Deville, Christelle ULg; Elmoualij, Benaïssa ULg et al

in BMC Cell Biology (2006), 7

Background: The absorptive and goblet cells are the main cellular types encountered in the intestine epithelium. The cell lineage Caco-2 is a model commonly used to reproduce the features of the bowel ... [more ▼]

Background: The absorptive and goblet cells are the main cellular types encountered in the intestine epithelium. The cell lineage Caco-2 is a model commonly used to reproduce the features of the bowel epithelium. However, there is a strong debate regarding the value of Caco-2 cell culture to mimick in vivo situation. Indeed, some authors report in Caco-2 a low paracellular permeability and an ease of access of highly diffusible small molecules to the microvilli, due to an almost complete lack of mucus. The HT29-5M21 intestinal cell lineage is a mucin-secreting cellular population. A co-culture system carried out in a serum-free medium and comprising both Caco-2 and HT29-5M21 cells was developed. The systematic use of a co-culture system requires the characterization of the monolayer under a given experimental procedure. Results: In this study, we investigated the activity and localization of the alkaline phosphatase and the expression of IAP and MUC5AC genes to determine a correlation between these markers and the cellular composition of a differentiated monolayer obtained from a mixture of Caco-2 and HT29-5M21 cells. We observed that the culture conditions used ( serum-free medium) did not change the phenotype of each cell type, and produced a reproducible model. The alkaline phosphatase expression characterizing Caco-2 cells was influenced by the presence of HT29-5M21 cells. Conclusion: The culture formed by 75% Caco-2 and 25% HT29-5M21 produce a monolayer containing the two main cell types of human intestinal epithelium and characterized by a reduced permeability to macromolecules. [less ▲]

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See detailDevelopment of a simple approach for ultrasensitive detection of bisphenols by multiplexed surface enhanced Raman scattering
De Bleye, Charlotte ULg; Dumont, Elodie ULg; Netchacovitch, Lauranne ULg et al

Poster (2015, July 13)

A very simple, cheap and fast SERS method using functionalized silver nanoparticles was developed to detect bisphenols. This method was applied for the semi-quantitative detection of bisphenol A (BPA ... [more ▼]

A very simple, cheap and fast SERS method using functionalized silver nanoparticles was developed to detect bisphenols. This method was applied for the semi-quantitative detection of bisphenol A (BPA), bisphenol B (BPB) and bisphenol F (BPF) separately. Afterwards, a feasibility study of performing a multiplex SERS detection of BPA, BPB and BPF was successfully carried out. Finally, this developed method was applied on real samples which were solutions comprising cash receipts collected from different stores. [less ▲]

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See detailDevelopment of a simplified Building-HVAC System model with the help of an equation solver
Bertagnolio, Stéphane ULg; Lemort, Vincent ULg; Lebrun, Jean ULg

Conference (2008, June 25)

This seminar shows how modeling and simulation environments that are built using equation-based solvers can benefit the building energy community. Such modeling and simulation environments separate the ... [more ▼]

This seminar shows how modeling and simulation environments that are built using equation-based solvers can benefit the building energy community. Such modeling and simulation environments separate the formulation of a model’s underlying physics from its numerical solution algorithms. This allows input-output free modeling, it leads to faster model development time and it facilitates model reuse for different applications. Equation-based modeling and simulation environments are frequently used in various industrial sectors. For building energy simulation, however, they have not yet enjoyed wide-spread use. This seminar encourages discussions to understand their applicability, advantages and limitations for the building energy community. [less ▲]

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See detailDevelopment of a simplified measuring procedure for ammonia emission from agricultural constructions
Vranken, Erik; Cabaraux, Jean-François ULg; Berckmans, Daniel

in Berckmans, D.; Vandermeulen, J. (Eds.) Precision Livestock Farming ‘13 (2013)

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See detailDevelopment of a small-scale scroll expander-based ORC
Quoilin, Sylvain ULg

Conference (2009, May 19)

Detailed reference viewed: 19 (0 ULg)
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See detailDevelopment of a specific serological test and an efficient subunit vaccine to control bovine leukemia virus infection.
Portetelle, Daniel ULg; Burny, Arsène; Desmettre, Philippe et al

in Developments in Biological Standardization (1990), 72(3), 81-90

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See detailDevelopment of a Standard Reference Material for Metabolomics Research
Phinney, K; Ballihaut, G; Bedner, M et al

in Analytical Chemistry (2013), 85(24), 11732-11738

The National Institute of Standards and Technology (NIST), in collaboration with the National Institutes of Health (NIH), has developed a Standard Reference Material (SRM) to support technology ... [more ▼]

The National Institute of Standards and Technology (NIST), in collaboration with the National Institutes of Health (NIH), has developed a Standard Reference Material (SRM) to support technology development in metabolomics research. SRM 1950 Metabolites in Human Plasma is intended to have metabolite concentrations that are representative of those found in adult human plasma. The plasma used in the preparation of SRM 1950 was collected from both male and female donors, and donor ethnicity targets were selected based upon the ethnic makeup of the U.S. population. Metabolomics research is diverse in terms of both instrumentation and scientific goals. This SRM was designed to apply broadly to the field, not towards specific applications. Therefore, concentrations of approximately 100 analytes, including amino acids, fatty acids, trace elements, vitamins, hormones, selenoproteins, clinical markers, and perfluorinated compounds (PFCs), were determined. Value assignment measurements were performed by NIST and the Centers for Disease Control and Prevention (CDC). SRM 1950 is the first reference material developed specifically for metabolomics research. [less ▲]

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See detailDevelopment of a statistical reduction method for the Palomar Fiber Nuller
Hanot, Charles ULg; Mennesson, Bertrand; Serabyn, Eugene et al

in Danchi, W. C.; Delplancke, F.; Rajagopal, J. K. (Eds.) Optical and Infrared Interferometry II (2010, July)

A unique statistical data analysis method has been developed for reducing nulling interferometry data. The idea is to make use of the statistical distributions of the fluctuating null depths and beam ... [more ▼]

A unique statistical data analysis method has been developed for reducing nulling interferometry data. The idea is to make use of the statistical distributions of the fluctuating null depths and beam intensities to retrieve the astrophysical null depth in the presence of fluctuations. The approach yields an accuracy much better than is possible with standard data reduction methods, because the accuracy of the null depth is not limited by the sizes of the phase and intensity errors but by the uncertainties on their statistical distributions. The result is an improvement in the instrumental null depth measurement limit of roughly an order of magnitude. We show in this paper that broadband null depths of 10[SUP]-4[/SUP] can be measured in the lab with our infrared Fiber Nuller without achromatic phase shifters. On sky results are also dramatically improved, with measured contrasts up to a couple of 10[SUP]-4[/SUP] with our instrument mounted on the Hale telescope at the Palomar Observatory. This statistical analysis is not specific to our instrument and may be applicable to other interferometers. [less ▲]

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See detailDevelopment of a strategy to study toxicodynamic of pollutants in spawning sea turtles
Dyc, Christelle ULg; Bouquegneau, Jean-Marie ULg; De Pauw-Gillet, Marie-Claire ULg et al

in Proceedings of the 29th Annual Symposium on Sea Turtle Biology and Conservation (2009)

Detailed reference viewed: 15 (2 ULg)
See detailDevelopment of a strategy to study toxicodynamic of pollutants in spawning sea turtles from French West Indies (Guadeloupe and Martinique)
Dyc, Christelle ULg; Debier, Cathy; Thomé, Jean-Pierre ULg et al

Conference (2008, November)

Sea turtles including the green turtle Chelonia mydas and the hawksbill turtle Eretmochelys imbricata are critically endangered species, facing different factors as marine pollution. There is a blatant ... [more ▼]

Sea turtles including the green turtle Chelonia mydas and the hawksbill turtle Eretmochelys imbricata are critically endangered species, facing different factors as marine pollution. There is a blatant lack of data dealing with toxicants such as metals and persistent organic pollutants (POPs) in sea turtles. We developed a strategy to apprehend levels, effects and transfer to offspring of several pollutants in sea turtles. Sampling of blood, subcutaneous tissue and eggs of 15 gravid C. mydas and E. imbricata was carried out between July and September 2008 in Martinique (Diamant’s beach) and Guadeloupe (Petite Terre and Marie-Galante). Blood was collected from the dorso-cervical sinus and subcutaneous tissue was sampled in shoulder of the spawning females using a 5 mm biopsy punch (Kai Europe GmbH, Germany). Total blood and serum were successfully taken for metal, POP and biomarker investigations. T-mercury was analyzed by DMA milestones while PCBs, DDT and chlordecone were analyzed by EDC Ni63 high performance gas chromatography HPLC. Samples of serum were analyzed for vitamins (A and E) by HPLC and for thyroid hormones (triiodothyronine and thyroxine) by radioimmunoassay. In parallel to this field study, a cell model using 3T3-L1 cell line was built up to test in vitro effects of PCBs and mercury as well as the relationship between in vitro exposure and fat mobilization. Preliminary results showed a dose-response relationship between increased Aroclor 1234 and 1252 concentrations (0.5 ppb, 1 ppb and 1.5 ppb) and adipocyte mortality (Nucleocounter). The strategy we propose here will bring further insights on levels and potential impact of pollutants on female sea turtles and their offspring. [less ▲]

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See detailDevelopment of a strategy to study toxicodynamic of pollutants in spawning sea turtles from the French West Indies
Dyc, Christelle ULg; Bouquegneau, Jean-Marie ULg; De Pauw-Gillet, Marie-Claire ULg et al

Poster (2008, October)

Sea turtles including the green turtle Chelonia mydas and the hawksbill turtle Eretmochelys imbricata are critically endangered species, facing different factors as marine pollution. There is a blatant ... [more ▼]

Sea turtles including the green turtle Chelonia mydas and the hawksbill turtle Eretmochelys imbricata are critically endangered species, facing different factors as marine pollution. There is a blatant lack of data dealing with toxicants such as metals and persistent organic pollutants (POPs) in sea turtles. We developed a strategy to apprehend levels, effects and transfer to offspring of several pollutants in sea turtles. Sampling of blood, subcutaneous tissue and eggs of 15 gravid C. mydas and E. imbricata was carried out between July and September 2008 in Martinique (Diamant’s beach) and Guadeloupe (Petite Terre and Marie-Galante). Blood was collected from the dorso-cervical sinus and subcutaneous tissue was sampled in shoulder of the spawning females using a 5 mm biopsy punch (Kai Europe GmbH, Germany). Total blood and serum were successfully taken for metal, POP and biomarker investigations. T-mercury was analyzed by DMA milestones while PCBs, DDT and chlordecone were analyzed by EDC Ni63 high performance gas chromatography HPLC. Samples of serum were analyzed for vitamins (A and E) by HPLC and for thyroid hormones (triiodothyronine and thyroxine) by radioimmunoassay. In parallel to this field study, a cell model using 3T3-L1 cell line was built up to test in vitro effects of PCBs and mercury as well as the relationship between in vitro exposure and fat mobilization. Preliminary results showed a dose-response relationship between increased Aroclor 1234 and 1252 concentrations (0.5 ppb, 1 ppb and 1.5 ppb) and adipocyte mortality (Nucleocounter). The strategy we propose here will bring further insights on levels and potential impact of pollutants on female sea turtles and their offspring. [less ▲]

Detailed reference viewed: 74 (11 ULg)
See detailDevelopment of a Substitute Mixture for Diesel Fuel
Reiter, A M; Wallek, T; Mair-Zelenka, P et al

Conference (2012)

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See detailDevelopment of a subwavelength grating vortex coronagraph of topological charge 4 (SGVC4)
Delacroix, Christian ULg; Absil, Olivier ULg; Carlomagno, Brunella ULg et al

in Ramsay, Suzanne; McLean, Ian; Takami, Hideki (Eds.) Ground-based and Airborne Instrumentation for Astronomy V (2014, July 08)

One possible solution to achieve high contrast direct imaging at a small inner working angle (IWA) is to use a vector vortex coronagraph (VVC), which provides a continuous helical phase ramp in the focal ... [more ▼]

One possible solution to achieve high contrast direct imaging at a small inner working angle (IWA) is to use a vector vortex coronagraph (VVC), which provides a continuous helical phase ramp in the focal plane of the telescope with a phase singularity in its center. Such an optical vortex is characterized by its topological charge, i.e., the number of times the phase accumulates 2pi radians along a closed path surrounding the singularity. Over the past few years, we have been developing a charge-2 VVC induced by rotationally symmetric subwavelength gratings (SGVC2), also known as the Annular Groove Phase Mask (AGPM). Since 2013, several SGVC2s (or AGPMs) were manufactured using synthetic diamond substrate, then validated on dedicated optical benches, and installed on 10-m class telescopes. Increasing the topological charge seems however mandatory for cancelling the light of bright stars which will be partially resolved by future Extremely Large Telescopes in the near-infrared. In this paper, we first detail our motivations for developing an SGVC4 (charge 4) dedicated to the near-infrared domain. The challenge lies in the design of the pattern which is unrealistic in the theoretically perfect case, due to state-of-the-art manufacturing limitations. Hence, we propose a new realistic design of SGVC4 with minimized discontinuities and optimized phase ramp, showing conclusive improvements over previous works in this field. A preliminary validation of our concept is given based on RCWA simulations, while full 3D finite-difference time-domain simulations (and eventually laboratory tests) will be required for a final validation. [less ▲]

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See detailDevelopment of a sustained release bolus for lambs
Evrard, Brigitte ULg; Dechesne, Jean-Paul; Fagetton, Xavier et al

Conference (1990, March)

Detailed reference viewed: 8 (0 ULg)
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See detailDevelopment of a sustained release bolus for sheep : "in vitro" and "in vivo" results
Evrard, Brigitte ULg; Dechesne, Jean-Paul; Fagetton, Xavier et al

in Proceedings of 19th International Symposium on controlled release of Bioactive Materials (1992)

Detailed reference viewed: 5 (2 ULg)