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See detailDevelopment and application of a SYBR Green RT-PCR for first line screening and quantification of porcine sapovirus infection
Mauroy, Axel ULg; Van der Poel, W.H.M.; Hakze-Van der Honing, R. et al

in BMC Veterinary Research (2012), 8

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See detailDevelopment and application of high-resolution petrography on resin-impregnated Holocene peat columns to detect and analyse tephras, cryptotephras, and other materials
De Vleeschouwer, François ULg; van Vliet-Lanoe, Brigitte; Fagel, Nathalie ULg et al

in Quaternary International (2008), 178

We describe the potential for high-resolution detection, observation and chemical analysis of tephras and cryptotephras in freeze-dried and resin-impregnated peat sections. Special attention is drawn to ... [more ▼]

We describe the potential for high-resolution detection, observation and chemical analysis of tephras and cryptotephras in freeze-dried and resin-impregnated peat sections. Special attention is drawn to tephra grain alteration products, sensitive to standard lab treatment, but preserved using an impregnation technique developed here. Resulting blocks and thin sections provide a continuous archive of tephras that can be investigated through a wide range of non-destructive techniques encompassing polarising and fluorescence microscopy, scanning electron microscopy with/without elemental analysis, and XRF-based core scanning. (c) 2007 Elsevier Ltd and INQUA. All rights reserved. [less ▲]

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See detailDevelopment and application of multiple internal reference (housekeeper) gene assays for accurate normalisation of canine gene expression studies
Peters, I. R.; Peeters, Dominique ULg; Helps, C. R. et al

in Veterinary Immunology and Immunopathology (2007), 117(1-2), 55-66

Measurement of mRNA expression by real-time RT-PCR (QRT-PCR) has proven to be an important and powerful tool for the investigation of the pathogenesis of inflammatory and immune-mediated diseases in many ... [more ▼]

Measurement of mRNA expression by real-time RT-PCR (QRT-PCR) has proven to be an important and powerful tool for the investigation of the pathogenesis of inflammatory and immune-mediated diseases in many species. This methodology has proven particularly valuable in the dog, a species for which there are currently few specific antibodies for measurement of relevant proteins. Internal control (housekeeper) mRNAs are widely used for normalisation of QRT-PCR results. The validation and use of multiple internal control mRNAs for increased accuracy of normalisation has been described for humans and rodents. The aims of this study were to develop QRT-PCR assays for 11 potential internal control mRNAs in the dog (ACTB, B2M, G3PDH, HMBS, HPRT1, RPL 13A, RPL32, RPS 18, SDHA, TBP and YWAZ) and validate their use with bone marrow, colon, duodenum, heart, kidney, liver, lung, lymph node, skeletal muscle, pancreas, spleen and stomach from seven dogs. Endoscopic biopsies of the superficial duodenal mucosa were also obtained from nine dogs suffering from chronic gastro-oesophageal disease. The most stably expressed genes varied in the tissues examined. RPL13A and RPL32 (both components of the 60S ribosomal subunit) were the most stably expressed genes in the majority of the tissues examined, whereas ACTB and B2M were the least stable. Distinct internal control genes were shown to be most appropriate for use in full-thickness versus superficial mucosal biopsies of the duodenum. The results of this study indicate that there are no universal control genes for gene expression studies in canine tissues. It is important to use multiple internal control genes based upon a survey of potential control genes applied to representative samples from different disease groups, culture conditions and/or time points in an experimental study. (C) 2007 Elsevier B.V. All rights reserved. [less ▲]

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See detailDevelopment and application of test cases for comparing vertical ground heat exchangers models
Bernier, Michel; Kummert, Michael; Bertagnolio, Stéphane ULg

in Proceedings of the 10th International IBPSA Conference, Beijing, China (2007, September)

The main objective of this paper is to establish a set of test cases for analytical verifications and intermodel comparisons of ground heat exchanger (GHX) models used in building simulation programs ... [more ▼]

The main objective of this paper is to establish a set of test cases for analytical verifications and intermodel comparisons of ground heat exchanger (GHX) models used in building simulation programs. Several test cases are suggested. They range from steady-state heat rejection in a single borehole to varying hourly loads with large yearly thermal imbalance in multiple borehole configurations. The usefulness of the proposed test cases is illustrated by running them with different GHX models. This comparison exercise has shown that 1-D models compare favourably well with the more elaborate 3-D models for relatively small simulation periods. The cyclic heat rejection/collection test has revealed some small deficiency in the load aggregation scheme of a particular model. Finally, the use of the asymmetric (cooling dominated) load profile test case for a bore field composed of 100 boreholes revealed that the borehole wall temperature predicted by two GHX models can differ by as much as 10oC after a 30 year simulation. [less ▲]

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See detailThe development and applications of a ground-based fiber nulling coronagraph
Martin, Stefan; Serabyn, Eugene; Liewer, Kurt et al

in Optical and Infrared Interferometry (2008, July)

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See detailDevelopment and assessment of the Osteoporosis Index of Risk (OSIRIS) to facilitate selection of women for bone densitometry
Sedrine, W. B.; Chevallier, T.; Zegels, Brigitte ULg et al

in Gynecological Endocrinology : The Official Journal of the International Society of Gynecological Endocrinology (2002), 16(3), 245-250

A simple questionnaire would be useful to identify individuals most in need of bone mineral density (BMD) testing. We designed a new predictive model and risk assessment instrument based on an extensive ... [more ▼]

A simple questionnaire would be useful to identify individuals most in need of bone mineral density (BMD) testing. We designed a new predictive model and risk assessment instrument based on an extensive review of the literature evaluating risk factors for osteoporosis, and tested its performance in a large cohort of postmenopausal women in whom BMD was measured by dual x-ray absorptiometry. In total, 1303 postmenopausal women from an outpatient osteoporosis clinic participated in this study. The Osteoporosis Index of Risk (OSIRIS) is based on four variables: age, body weight, current hormone replacement therapy use and history of previous low impact fracture. The sensitivity and specificity for an OSIRIS value of +1 were respectively 78.5% and 51.4%. The AUC under the ROC curve of OSIRIS was 0.71. Three categories were arbitrarily created using OSIRIS, with cutoff of +1 and -3. The low risk category (OSIRIS > +1) represented 41% of all women; only 7% of the women in this category had osteoporosis. The prevalence of osteoporosis was very high (66%) among the group at high risk (OSIRIS < -3 representing 15% of all women). The prevalence of osteoporosis was 39% in the intermediate risk group (-3 < OSIRIS < +1, 44% of all women). In conclusion, OSIRIS is a simple index based on four easy-to-collect variables from postmenopausal women, it shows a high degree of accuracy, and performed well for classifying the degree of risk of osteoporosis in western European women of Caucasian lineage. Based on this instrument it is possible to propose a strategy that would initiate treatment in women with very high risk, postpone BMD measurement in women with low risk and limit BMD measurement to women with intermediate risk of osteoporosis, this would spare more than 55% of the densitometry bill compared with a mass screening scenario. [less ▲]

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See detailDevelopment and characterization of microsatellite loci in Pericopsis elata (Fabaceae) using a cost-efficient approach
Micheneau, Claire; Dauby, Gilles; Bourland, Nils ULg et al

in American Journal of Botany (2011)

Premise of the study : Microsatellite loci were developed in the endangered Pericopsis elata using a combination of low-cost procedures. Methods and Results : Microsatellite isolation was performed ... [more ▼]

Premise of the study : Microsatellite loci were developed in the endangered Pericopsis elata using a combination of low-cost procedures. Methods and Results : Microsatellite isolation was performed simultaneously on three distinct species through a newly available procedure that associates multiplex microsatellite enrichment and next-generation sequencing, allowing the rapid and low-cost development of microsatellite-enriched libraries through the use of a 1/32nd GS-FLX plate. Genotyping using M13-like label- ing in multiplexed reactions allowed additional cost savings. From 72 primers selected for initial screening, 21 positively amplified P. elata , and 11 showed polymorphism with two to 11 alleles per locus and a mean value of 5.4 alleles per locus. Conclusions : These microsatellite loci will be useful to further investigate the level of genetic variation within and between natural populations of P. elata in Africa. [less ▲]

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See detailDevelopment and characterization of SSR markers for pomegranate (Punica granatum L.) using an enriched library
Hasnaoui, Nejib ULg; Buonamici, Anna; Sebastiani, Federico et al

in Conservation Genetics Resources (2010), 2

In the present work, we report the development of 11 microstallite markers (SSR) for Punica granatum. Evaluated on a set of 27 pomegranate accessions sampled in Tunisia, they displayed 25 alleles, with ... [more ▼]

In the present work, we report the development of 11 microstallite markers (SSR) for Punica granatum. Evaluated on a set of 27 pomegranate accessions sampled in Tunisia, they displayed 25 alleles, with number of alleles per locus ranging between 1 and 4, and an observed heterozygosity from 0.037 and 0.592. This set of SSR markers can be very useful for studies dealing with genetic diversity assessment of germplasm, with cultivars/varieties fingerprinting and pedigree analysis of this economically important fruit species. [less ▲]

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See detailDevelopment and epidemiological evaluation of a monoclonal ELISA detecting Bovine Viral Diarrhœa Pestivirus antigens in field blood samples of persistently infected cattle
Mignon, Bernard ULg; Dubuisson, J.; Waxweiler, S. et al

in Steven, Edwards (Ed.) ESVV-Proceedings of the second symposium on pestiviruses (1993)

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See detailDevelopment and evaluation in vitro and in vivo of injectable hydrolipidic gels with sustained-release properties for the management of articular pathologies such as osteoarthritis.
Réeff, Jonathan; Oprenyeszk, Frédéric ULg; Franck, Thierry ULg et al

in International Journal of Pharmaceutics (2015), 490

This study aimed to evaluate glycerol monooleate (GMO) as a carrier to develop viscoelastic and injectable sustained-release drug delivery systems. The potential pro- and antioxidant activity of the ... [more ▼]

This study aimed to evaluate glycerol monooleate (GMO) as a carrier to develop viscoelastic and injectable sustained-release drug delivery systems. The potential pro- and antioxidant activity of the developed hydrolipidic gels were evaluated by measuring the production of ROS by polymorphonuclear leukocytes (PMNs). In addition, the biocompatibility and effectiveness of two selected gel candidates were evaluated in vivo by evaluating the benefit of a single intraarticular injection of these new treatments in a model of osteoarthritis in rabbits. The in vitro study demonstrated that the carrier F1 did not have a pro-oxidative effect and even protected PMNs against natural auto-activation, regardless of the incorporation of either clonidine chlorhydrate or betamethasone dipropionate. The in vivo study demonstrated that F1 and F1-BDP induced a loss of cartilage quality in comparison to the control and reference groups but that the lesions of cartilage observed were generally mild, with not much full-depth erosion. Moreover, no exacerbating inflammation was observed when considering the synovial membranes and the PGE2 and CRP levels. These results seemed to demonstrate that the sustained-release formulation based on GMO could be well-tolerated after intraarticular injection. Moreover, it could have the potential to prevent inflammatory conditions while sustaining drug activity locally over weeks. [less ▲]

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See detailDevelopment and Evaluation of a VTOL Observation Platform
Buysschaert, F.; Yacoubi, M.; Alami, A. et al

in Proceedings of the 27th International Congress of the Aerospace Sciences (2010, September)

An aerodynamic study of the ULB-developed ducted rotor MAV using the results of full-scale wind tunnel tests allowed the determination of the platform’s positive speed envelope, power requirements and ... [more ▼]

An aerodynamic study of the ULB-developed ducted rotor MAV using the results of full-scale wind tunnel tests allowed the determination of the platform’s positive speed envelope, power requirements and endurance characteristics for ISA sea level conditions. In this study, the power consumption appears to be majorly depending on the rotor rotational speed, while an increase in horizontal speed results in a duct operating more as a circular wing, also unveiling a power bucket as is the case with conventional helicopters. A positive influence of the ground proximity on the total thrust has been monitored. [less ▲]

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See detailDevelopment and evaluation of an automated atlas-based image analysis method for microPET studies of the rat brain.
Rubins, Daniel J.; Melega, William P.; Lacan, Goran et al

in NeuroImage (2003), 20(4), 2100-18

An automated method for placement of 3D rat brain atlas-derived volumes of interest (VOIs) onto PET studies has been designed and evaluated. VOIs representing major structures of the rat brain were ... [more ▼]

An automated method for placement of 3D rat brain atlas-derived volumes of interest (VOIs) onto PET studies has been designed and evaluated. VOIs representing major structures of the rat brain were defined on a set of digitized cryosectioned images of the rat brain. For VOI placement, each PET study was registered with a synthetic PET target constructed from the VOI template. Registration was accomplished with an automated algorithm that maximized the mutual information content of the image volumes. The accuracy and precision of this method for VOI placement was determined using datasets from PET studies of the striatal dopamine and hippocampal serotonin systems. Each evaluated PET study could be registered to at least one synthetic PET target without obvious failure. Registration was critically dependent upon the initial position of the PET study relative to the synthetic PET target, but not dependent on the amount of synthetic PET target smoothing. An evaluation algorithm showed that resultant radioactivity concentration measurements of selected brain structures had errors=2% due to misalignment with the corresponding VOI. Further, radioligand binding values calculated from these measurements were found to be more precise than those calculated from measurements obtained with manually drawn regions of interest (ROIs). Overall, evaluation results demonstrated that this atlas-derived VOI method can be used to obtain unbiased measurements of radioactivity concentration from PET studies. Its automated features, and applicability to different radioligands and brain regions, will facilitate quantitative rat brain PET assessment procedures. [less ▲]

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See detailThe development and experimental validation of a reduced ternary kinetic mechanism for the auto-ignition at HCCI conditions, proposing a global reaction path for ternary gasoline surrogates
Machrafi, Hatim ULg; Cavadias, Simeon; Amouroux, Jacques

in Fuel Processing Technology (2009), 90(2), 247-263

To acquire a high amount of information of the behaviour of the Homogeneous Charge Compression Ignition (HCCI) auto-ignition process, a reduced surrogate mechanism has been composed out of reduced n ... [more ▼]

To acquire a high amount of information of the behaviour of the Homogeneous Charge Compression Ignition (HCCI) auto-ignition process, a reduced surrogate mechanism has been composed out of reduced n-heptane, iso-octane and toluene mechanisms, containing 62 reactions and 49 species. This mechanism has been validated numerically in a OD HCCI engine code against more detailed mechanisms (inlet temperature varying from 290 to 500 K, the equivalence ratio from 0.2 to 0.7 and the compression ratio from 8 to 18) and experimentally against experimental shock tube and rapid compression machine data from the literature at pressures between 9 and 55 bar and temperatures between 700 and 1400 K for several fuels: the pure compounds n-heptane, iso-octane and toluene as well as binary and ternary mixtures of these compounds. For this validation, stoichiometric mixtures and mixtures with an equivalence ratio of 0.5 are used. The experimental validation is extended by comparing the surrogate mechanism to experimental data from an HCCI engine. A global reaction pathway is proposed for the auto-ignition of a surrogate gasoline, using the surrogate mechanism, in order to show the interactions that the three compounds can have with one another during the auto-ignition of a ternary mixture. (C) 2008 Elsevier B.V. All rights reserved. [less ▲]

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See detailDevelopment and experimental validation of an Organic Rankine cycle model
Lemort, Vincent ULg; Quoilin, Sylvain ULg; Cuevas, Critian et al

(2007)

This paper presents both a numerical model of an Organic Rankine Cycle (ORC) and an experimental study carried out on a prototype of such a cycle working with refrigerant HCFC-123, and whose heat sources ... [more ▼]

This paper presents both a numerical model of an Organic Rankine Cycle (ORC) and an experimental study carried out on a prototype of such a cycle working with refrigerant HCFC-123, and whose heat sources consist in two hot air flows. The ORC model s built by connecting different sub-models: the heat exchanger models, a volumetric pump model and a scroll expander model. Measured performances of the ORC prototype are presented and allow the validation of the ORC model. This model is finally used to investigate potential improvements of the prototype. [less ▲]

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See detailDevelopment and Identification of a Closed-Loop Model of the Cardiovascular System Including the Atria
Pironet, Antoine ULg; Revie, James A.; Paeme, Sabine ULg et al

Conference (2012, August 31)

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See detailDevelopment and Identification of a Closed-Loop Model of the Cardiovascular System Including the Atria
Pironet, Antoine ULg; Revie, James A.; Paeme, Sabine ULg et al

in Proceedings of the 8th IFAC Symposium on Biological and Medical Systems (2012, August)

Detailed reference viewed: 45 (10 ULg)