Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailCyclodextrins as a potential carrier in drug nebulization.
Evrard, Brigitte ULiege; Bertholet, P.; Guéders, Maud ULiege et al

in Journal of Controlled Release (2004), 96(3), 403-10

The inhalation route is widely studied for many drug applications focusing on either local or systemic distributions. One matter of concern is the solubilization of hydrophobic drugs. We have studied the ... [more ▼]

The inhalation route is widely studied for many drug applications focusing on either local or systemic distributions. One matter of concern is the solubilization of hydrophobic drugs. We have studied the feasibility of using different cyclodextrins (CDs) to elaborate pharmaceutical formulations for the inhalation route and tested the short-term toxicity of such formulations administered by inhalation to C57BL/6 mice. We have shown that HP-beta-CD, gamma-CD, as well as RAMEB aqueous solutions can undergo aerosolization and that the resulting droplet-size ranges are compatible with pulmonary deposition. In vivo, we have demonstrated that short-term exposure to inhaled HP-beta-CD, gamma-CD and RAMEB solutions are non-toxic after assessing bronchoalveolar lavage (BAL), lung and kidney histology, bronchial responsiveness to methacholine and blood urea. The only change noted is a slight increase in lymphocyte count in the BAL after HP-beta-CD and gamma-CD inhalation. We conclude that CDs are useful in significantly enhancing the solubility of apolar drugs with a view to inhalation therapy although an increase in lymphocyte counts in the BAL after CDs inhalations needs further investigations. [less ▲]

Detailed reference viewed: 62 (14 ULiège)
Peer Reviewed
See detailCyclodextrins as topical permeation enhancers : in vitro and in vivo testing
Henry de Hassonville, S.; Christiaens, B.; Piel, Géraldine ULiege et al

Poster (2003)

Detailed reference viewed: 17 (2 ULiège)
Full Text
Peer Reviewed
See detailCyclodimerization by ring-closing metathesis: synthesis, computational and biological evaluation of novel bis-azetidinyl-macrocycles
Sliwa, Aline; Dive, Georges ULiege; Habib Jiwan, Jean-Louis et al

in Tetrahedron (2010), 66

Detailed reference viewed: 26 (5 ULiège)
Peer Reviewed
See detailCyclopentenone prostaglandins at low concetrations exert pro-inflammatory effects through oxidative stress-induced ERK1/2 activation
Bureau, Fabrice ULiege; Desmet, Christophe ULiege; Mélotte, C. et al

in Proceedings: Spring Meeting of the Belgian Society of Physiology and Pharmacology (2002)

Detailed reference viewed: 14 (4 ULiège)
Full Text
Peer Reviewed
See detailCyclophosphamide inhibits the generation and function of CD8(+) regulatory T cells
Conteduca, Giuseppina ULiege

in Human Immunology (2012), 73(3), 207-13

CD8(+) regulatory T cells (Treg) and CD4(+)CD25(+) Treg infiltrate human cancers, thus favoring tumor immune escape. Therefore, in the setting of antitumor therapeutic protocols, it is important to ... [more ▼]

CD8(+) regulatory T cells (Treg) and CD4(+)CD25(+) Treg infiltrate human cancers, thus favoring tumor immune escape. Therefore, in the setting of antitumor therapeutic protocols, it is important to associate antitumor treatment with agents that are able to inhibit Treg function. Cyclophosphamide (CY) has been demonstrated to be effective in counteracting CD4(+)CD25(+) Treg activity. Hence, we tested its inhibitory efficacy on human CD8(+) Treg. Because CY is a prodrug, 4-hydroperoxycyclophosphamide (4-HC), a derivative of CY that in aqueous solution is converted to 4-hydroxycyclophosphamide, an active metabolite of CY, was used. 4-HC significantly inhibited CD8(+) Treg generation and function but only at the higher tested concentration (0.5 μg/mL), that is, in the therapeutic range of the drug. The lower 4-HC concentration tested (0.1 μg/mL) was almost ineffective. 4-HC inhibitory effects were related to apoptosis/necrosis induction. When CD8(+)CD28(+) non-Treg were analyzed for comparative purposes, significantly lower cytotoxic rates among these cells were observed than among CD8(+) Treg, which were differentiated because they did not express the CD28 molecule. These data demonstrate that CD8(+) Treg are inhibited through cytotoxic phenomena by CY, thus supporting the use of this drug at adequate concentrations and schedules of administration as a Treg inhibitor in combinatorial chemo- or immunotherapeutic anticancer protocols. [less ▲]

Detailed reference viewed: 20 (1 ULiège)
Peer Reviewed
See detailCyclosporin A, rapamycin and FK506 decrease prolactin release from rat pituitary cells in primary culture
Wera, S.; Zheng, L.; Hooghe-Peters, E. L. et al

in Endocrine Research (1995), 21(3), 623-33

It is at present well established that prolactin exerts a non-specific immunoactivating function. In this work we tested whether the immunosuppressant drugs cyclosporin A, FK506 and rapamycin influence ... [more ▼]

It is at present well established that prolactin exerts a non-specific immunoactivating function. In this work we tested whether the immunosuppressant drugs cyclosporin A, FK506 and rapamycin influence prolactin release from rat pituitary cells in primary culture. The tested drugs had no effect on the prolactin release measured during a 2h incubation period, indicating that they do not influence the secretion of prolactin from intracellular stores into the culture medium. During longer incubation times (48h), however, prolactin release was diminished to 56% +/- 18 (10 microM cyclosporin A), 64% +/- 14 (1 microM rapamycin) or 64% +/- 7 (1 microM FK506), suggesting an effect on prolactin production. At these drug concentrations no toxic effects were observed. The data indicate that inhibition of pituitary prolactin synthesis might contribute to the immunosuppressant action of cyclosporin A, rapamycin and FK506. [less ▲]

Detailed reference viewed: 22 (0 ULiège)
Full Text
Peer Reviewed
See detailCyclosporin C Is the Main Antifungal Compound Produced by Acremonium Luzulae
Moussaif, M.; Jacques, Philippe ULiege; Schaarwachter, P. et al

in Applied and Environmental Microbiology (1997), 63(5), 1739-43

A strain of Acremonium luzulae (Fuckel) W. Gams was selected in screening new microorganisms for biological control of fruit postharvest diseases, especially gray and blue mold diseases on apples and ... [more ▼]

A strain of Acremonium luzulae (Fuckel) W. Gams was selected in screening new microorganisms for biological control of fruit postharvest diseases, especially gray and blue mold diseases on apples and strawberries. This strain manifests a very strong activity against a large number of phytopathogenic fungi. In this work, the product responsible for this antifungal activity was isolated from modified Sabouraud dextrose broth cultures of A. luzulae. It was purified to homogeneity by reverse-phase column chromatography. On the basis of UV, infrared, and 1H and 13C nuclear magnetic resonance spectra, mass spectral analysis, and the amino acid composition of the acid hydrolysates, the antibiotic was determined to be cyclosporin C. Cyclosporin C showed a broad-spectrum activity against filamentous phytopathogenic fungi but no activity against bacteria or yeasts. Its antifungal activity is only fungistatic. In contrast to Tolypocladium inflatum, another cyclosporin-producing strain, A. luzulae, did not produce additional cyclosporins. This was confirmed by in vivo-directed biosynthesis. [less ▲]

Detailed reference viewed: 27 (1 ULiège)
Full Text
Peer Reviewed
See detailCyclosporin-A differentially affects apoptosis during in vivo rat thymocyte maturation
Damoiseaux, JGMC; Defresne, Marie-Paule ULiege; Reutelingsperger, C. P. M. et al

in Scandinavian Journal of Immunology (2002), 56(4), 353-360

Maturation arrest and interference with selection are two well-documented effects of cyclosporin-A (CsA) on the thymus. We recently hypothesized that these effects are related and owing to the reduced T ... [more ▼]

Maturation arrest and interference with selection are two well-documented effects of cyclosporin-A (CsA) on the thymus. We recently hypothesized that these effects are related and owing to the reduced T-cell receptor (TCR)-CD3 complex-mediated signal transduction in thymocytes upon CsA treatment. In this hypothesis, the maturation arrest is the result of the additional depletion of thymocytes that normally survive by positive selection, whereas the impaired self-tolerance induction is caused by an increased survival of thymocytes that normally undergo negative selection. In this view, it is anticipated that CsA differentially affects thymocyte apoptosis during in vivo thymocyte maturation. Indeed, we report in this study a strong increase in apoptotic cells in the thymic cortex on in situ analysis. Simultaneously, the number of apoptotic cells had decreased at the cortico-medullary zone which is held to be the site for negative selection. Rapamycin (Rapa) also interferes with thymocyted maturation by inhibiting cytokine-driven proliferation. Hence, Rapa preferentially affects the early maturational stages of thymoctye development and is considered not to alter thymocyte selection and subsequent apoptotic events. Indeed, the number of apoptotic events appears not to be altered. However, possibly owing to the decrease in cortical macrophages, the apoptotic cells revealed an atypical enumeration around blood vessels. Taken together, our results favour the hypothesis that the dominant effect of CsA on the thymus is the reduction of the TCR-CD3 complex-mediated signal transduction in thymocytes upon interaction with stromal cells. Furthermore, the preferential localization of apoptotic cells next to blood vessels upon Rapa administration may indicate that endothelial cells are a back-up system for the removal of apoptotic cells. [less ▲]

Detailed reference viewed: 10 (0 ULiège)
Full Text
Peer Reviewed
See detailCyclosporine a Inhibits Partially Spermine-Induced Differentiation but Not Cell Loss of Suckling Rat Small Intestine
Peulen, Olivier ULiege; Dandrifosse, Guy ULiege

in Digestive Diseases & Sciences (2000), 45(4), 750-4

The polyamines are of great importance in several biological processes, such as cell proliferation, and differentiation. The ingestion of spermine by suckling rats induces the precocious maturation of ... [more ▼]

The polyamines are of great importance in several biological processes, such as cell proliferation, and differentiation. The ingestion of spermine by suckling rats induces the precocious maturation of their small intestine. This phenomenon is preceded by a cell elimination at the villus tip. We hypothesize that these two phenomena could be mediated by the immune system and thus inhibited by an immunosuppressive agent such as cyclosporine A. Cyclosporine A inhibits, at least partially, the spermine-induced increase of the maltase- and sucrase-specific activities in the small intestine but failed to inhibit lactase-specific activity decrease and cell loss. Spermine does not act by the same mechanism in differentiation and in cell loss. Moreover, spermine acts in a different way on lactase-specific activity compared to maltase- or sucrase-specific activity. We hypothesize that spermine acts on differentiation by a T-cell/IL-2-dependent mechanism. [less ▲]

Detailed reference viewed: 14 (1 ULiège)
Full Text
Peer Reviewed
See detailCyclosporine, a P-glycoprotein modulator, increases [18F]MPPF uptake in rat brain and peripheral tissues: microPET and ex vivo studies.
Lacan, Goran; Plenevaux, Alain ULiege; Rubins, Daniel J. et al

in European Journal of Nuclear Medicine and Molecular Imaging (2008), 35(12), 2256-66

PURPOSE: Pretreatment with cyclosporine, a P-glycoprotein (P-gp) modulator increases brain uptake of 4-(2'-methoxyphenyl)-1-[2'-(N-2"-pyridinyl)-p-[(18)F]fluorobenzamido]ethylpiperaz ine ([(18)F]MPPF) for ... [more ▼]

PURPOSE: Pretreatment with cyclosporine, a P-glycoprotein (P-gp) modulator increases brain uptake of 4-(2'-methoxyphenyl)-1-[2'-(N-2"-pyridinyl)-p-[(18)F]fluorobenzamido]ethylpiperaz ine ([(18)F]MPPF) for binding to hydroxytryptamine(1A) (5-HT(1A)) receptors. Those increases were quantified in rat brain with in vivo microPET and ex vivo tissue studies. MATERIALS AND METHODS: Each Sprague-Dawley rat (n = 4) received a baseline [(18)F]MPPF microPET scan followed by second scan 2-3 weeks later that included cyclosporine pretreatment (50 mg/kg, i.p.). Maximum a posteriori reconstructed images and volumetric ROIs were used to generate dynamic radioactivity concentration measurements for hippocampus, striatum, and cerebellum, with simplified reference tissue method (SRTM) analysis. Western blots were used to semiquantify P-gp regional distribution in brain. RESULTS: MicroPET studies showed that hippocampus uptake of [(18)F]MPPF was increased after cyclosporine; ex vivo studies showed similar increases in hippocampus and frontal cortex at 30 min, and for heart and kidney at 2.5 and 5 min, without concomitant increases in [(18)F]MPPF plasma concentration. P-gp content in cerebellum was twofold higher than in hippocampus or frontal cortex. CONCLUSIONS: These studies confirm and extend prior ex vivo results (J. Passchier, et al., Eur J Pharmacol, 2000) that showed [(18)F]MPPF as a substrate for P-gp. Our microPET results showed that P-gp modulation of [(18)F]MPPF binding to 5-HT(1A) receptors can be imaged in rat hippocampus. The heterogeneous brain distribution of P-gp appeared to invalidate the use of cerebellum as a nonspecific reference region for SRTM modeling. Regional quantitation of P-gp may be necessary for accurate PET assessment of 5-HT(1A) receptor density when based on tracer uptake sensitive to P-gp modulation. [less ▲]

Detailed reference viewed: 81 (6 ULiège)
Full Text
Peer Reviewed
See detailCyclostratigraphic calibration of the Late Devonian time scale
Pas, Damien ULiege; Hinnov, Linda; Day, James E. (Day) et al

in 33 rd INTERNATIONAL MEETING OF SEDIMENTOLOGY, 16ème CONGRÈS FRANÇAIS DE SÉDIMENTOLOGIE (2017, October 13)

The Late Devonian period was affected by major, irreversible changes including two of the most severe biodiversity crises in Earth’s history, the so-called Kellwasser and Hangenberg Events (respectively ... [more ▼]

The Late Devonian period was affected by major, irreversible changes including two of the most severe biodiversity crises in Earth’s history, the so-called Kellwasser and Hangenberg Events (respectively at the Frasnian – Famennian and the Famennian – Carboniferous boundaries). Currently, hypotheses for the Late Devonian extinctions include sea-level fluctuations and regression, climate cooling, ocean anoxia, massive volcanism and/or bolide impact. Unfortunately, testing these hypotheses is impaired by a lack of sufficient temporal resolution in paleobiological, tectonic and proxy climate records. Recent advances in astronomical dating have improved the accuracy of the Frasnian time scale and part of the Famennian (De Vleeschouwer et al., 2012, 2013). However, the time duration of the Famennian stage remains poorly constrained even though this is the key to understanding cause-and-effect relationships of the Late Devonian greenhouse–icehouse transition and the onset of the end Famennian glaciation that ultimately led to the Hangenberg Event at the Devonian–Carboniferous boundary. During the Late Devonian an epieric sea in mid-continent North America occupied the Illinois Basin onto which a Late Frasnian – Early Carboniferous sequence of deep-shelf deposits was archived. A complete record of this sequence, mainly composed of marl, organic-rich shale, silty-shale and carbonate, is captured in three overlapping cores stored in the Iowa Geological Survey (H-30, Sullivan Slough and H-32). H-30 records the Frasnian-Famennian boundary, the Sullivan Slough covers almost all the Famennian (middle triangularis-upper expansa conodont zones) and H-32 spans the junction between the uppermost Famennian and lowermost Carboniferous. To have the best chance of capturing Milankovitch cycles (long- and short-eccentricity cycles as well as obliquity and precession), 2200 rock samples were collected at 5-cm intervals across the entire sequence. Magnetic susceptibility (MS) was measured on each sample and the preservation of paleoenvironmental/climatic information into the signal was verified through geochemical analyses (Ti, Al, Zr by XRF) and magnetic measurements (low temperature magnetic susceptibility and IRM acquisitions). Correlation and overlap between each core was made using available conodont biostratigraphy and then refined by comparing the trends in the MS and δ13C signals for critical intervals. To estimate the duration of the Famennian stage we applied multiple techniques on the MS signal (multitaper method, evolutive harmonic analysis and adaptive-weighted harmonic F-test). By combining these techniques, we identified highly stable 405-kyr cycles across the Sullivan Slough and the H-32 cores. 405-kyr cycles were not observed in H-30, but 100-kyr cycles were identified. A preliminary estimate of the duration for the Famennian stage using the 405-kyr cycle as chronometer constrains the duration of the Famennian stage to 13 ± 0.4 Myr with an average sedimentation rate of 0.5 cm/kyr across the sequence. [less ▲]

Detailed reference viewed: 20 (7 ULiège)
Full Text
See detailCyclostratigraphic implications of Devonian-climate astronomical forcing.
De Vleeschouwer, David; Da Silva, Anne-Christine ULiege; Boulvain, Frédéric ULiege et al

in Geological Society of America Abstracts with Programs (2010), 42(5), 540

Detailed reference viewed: 17 (1 ULiège)
See detailCyclostratigraphy and environmental magnetism approach on the Lochkovian and Pragian from the Praha region, Czech Republic.
Da Silva, Anne-Christine ULiege; Chadimova, L.; Hladil, J. et al

in International Association of Sedimentologists Meeting, 22-25th June 2015, Krakow, Abstract book (2015)

Detailed reference viewed: 14 (0 ULiège)
Full Text
Peer Reviewed
See detailCyclotheonellazoles A–C, Potent Protease Inhibitors from the Marine Sponge Theonella aff. swinhoei
Issac, Michal; Aknin, Maurice; Gauvin-Bialecki, Anne et al

in Journal of Natural Products (2017), 80

he extract of a sample of the sponge Theonella aff. swinhoei collected in Madagascar exhibited promising in vitro antiplasmodial activity. The antiplasmodial activity was ascribed in part to the known ... [more ▼]

he extract of a sample of the sponge Theonella aff. swinhoei collected in Madagascar exhibited promising in vitro antiplasmodial activity. The antiplasmodial activity was ascribed in part to the known metabolite swinholide A. Further investigation of the extract afforded three unusual cyclic peptides, cyclotheonellazoles A–C (1–3), which contain six nonproteinogenic amino acids out of the eight acid units that compose these natural products. Among these acids the most novel were 4-propenoyl-2-tyrosylthiazole and 3-amino-4-methyl-2-oxohexanoic acid. The structure of the compounds was elucidated by interpretation of the 1D and 2D NMR data, HRESIMS, and advanced Merfay’s techniques. The new compounds were found to be nanomolar inhibitors of chymotrypsin and sub-nanomolar inhibitors of elastase, but did not present antiplasmodial activity. [less ▲]

Detailed reference viewed: 24 (2 ULiège)
Full Text
Peer Reviewed
See detailCyclovirus CyCV-VN species distribution is not limited to Vietnam and extends to Africa
Garigliany, Mutien-Marie ULiege; Hagen, Ralf Mathias; Frickmann, Hagen et al

in Scientific Reports (2014), 4

Cycloviruses, small ssDNA viruses of the Circoviridae family, have been identified in the cerebrospinal fluid from symptomatic human patients. One of these species, cyclovirus-Vietnam (CyCV-VN), was shown ... [more ▼]

Cycloviruses, small ssDNA viruses of the Circoviridae family, have been identified in the cerebrospinal fluid from symptomatic human patients. One of these species, cyclovirus-Vietnam (CyCV-VN), was shown to be restricted to central and southern Vietnam. Here we report the detection of CyCV-VN species in stool samples from pigs and humans from Africa, far beyond their supposed limited geographic distribution. [less ▲]

Detailed reference viewed: 43 (6 ULiège)
See detailCylinder Seal Inscriptions of Samsi-Adad and his officials from Acemhöyük
Tunca, Önhan ULiege

in Anatolia and the Ancient Near East (1989)

Detailed reference viewed: 74 (2 ULiège)
Full Text
Peer Reviewed
See detailCymastrobus irvingii gen. et sp. nov., a new isotalean cone from the Late Devonian locality of Barraba, NSW Australia
Evreïnoff, M.; Meyer-Berthaud, B.; Decombeix, A.-L. et al

Conference (2017, July)

Detailed reference viewed: 15 (2 ULiège)