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See detailEffect of an anti-termite treatment on hindgut content metaproteome
Bauwens, Julien ULg; Fossépré, Marie; De Pauw, Edwin ULg et al

Conference (2014, July 16)

Introduction Termites are studied for many aspects of their biology. Historically known as pests in regard of human activities, these insects were more recently intensively investigated in the biofuel ... [more ▼]

Introduction Termites are studied for many aspects of their biology. Historically known as pests in regard of human activities, these insects were more recently intensively investigated in the biofuel context. Symbiotic interactions occurring along the termite gut are of particular interest for both of these scopes. In this study we compared hindgut metaproteome of termites fed with an anti-termite treatment and an untreated diet. Material & Methods Termites were fed with Whatman paper for 84 hours. For untreated and treated diet respectively, paper was moisten with water and a sub lethal suspension of tannins associated with boric acid (BAT). Termite hindgut content proteome was extracted and digested. Peptides were analyzed through nano-LC-ESI-MS/MS using an Orbitrap analyzer. Proteins identification was realized by Mascot search in a homemade termite protein sequences database. Non redundant peptides with a score above identity threshold were blasted against NCBI nr database and results of this blast were analyzed using MeGAn 5. Results High resolution mass spectrometry allowed identification of around 1500 and 1000 non redundant peptides respectively for water and ABT treatment. Peptides were parsed following their taxonomic and functional attribution in order to highlight differences in hindgut metabolism such as cellulose digestion or detoxification process. Identification of ubiquitous proteins also revealed differences in symbiotic populations balance. Conclusions Despite the sub lethal concentration for the anti-termite treatment, significant perturbation of hindgut metabolism was observed. Tannins are slightly repulsive for termites compared to boric acid alone, and this has to be taken in account. These results will be completed with biochemical assays, microscopic observations and 2D-DIGE MALDI-TOF/TOF experiment, analyzing potentially synergistic effect between boric acid and tannins. [less ▲]

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See detailEffect of an audiovisual message for tetanus booster vaccination broadcast in the waiting room
EUBELEN, Caroline; BRENDEL, Fannette; Belche, Jean-Luc ULg et al

in BMC Family Practice (2011), 12

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See detailEffect of an electric field on an intermittent granular flow
Mersch, Eric ULg; Lumay, Geoffroy ULg; Boschini, Frédéric ULg et al

in Physical Review. E : Statistical, Nonlinear, and Soft Matter Physics (2010), 81

Granular gravity driven flows of glass beads have been observed in a silo with a flat bottom. A dc high electric field has been applied perpendicularly to the silo to tune the cohesion. The outlet mass flow ... [more ▼]

Granular gravity driven flows of glass beads have been observed in a silo with a flat bottom. A dc high electric field has been applied perpendicularly to the silo to tune the cohesion. The outlet mass flow has been measured. An image subtraction technique has been applied to visualize the flow geometry and a spatiotemporal analysis of the flow dynamics has been performed. The outlet mass flow is independent of voltage, but a transition from funnel flow to rathole flow is observed. This transition is of probabilistic nature and an intermediate situation exists between the funnel and the rathole situations. At a given voltage, two kinds of flow dynamics can occur: a continuous flow or an intermittent flow. The electric field increases the probability to observe an intermittent flow. [less ▲]

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See detailEffect of an inactivated bluetongue serotype 8 vaccine on semen quality in rams.
Leemans, Jerome; Raes, Marianne; Saegerman, Claude ULg et al

in Veterinary Journal (2012), 193(2), 567-9

The aim of this study was to determine whether a single dose of an inactivated bluetongue virus serotype 8 (BTV-8) vaccine altered semen quality in rams. Twenty sexually mature rams were assigned to three ... [more ▼]

The aim of this study was to determine whether a single dose of an inactivated bluetongue virus serotype 8 (BTV-8) vaccine altered semen quality in rams. Twenty sexually mature rams were assigned to three experimental groups: two groups of four animals were vaccinated and a third group of four animals was unvaccinated. The first group included rams with a history of natural BTV-8 infection in 2007 and the second and third groups included BTV-8 naive rams. Semen was collected prior to vaccination and for 4months post-vaccination. There were no significant differences in semen quality traits, including motility and concentration of spermatozoa, and percentages of living, normal dead and abnormal dead spermatozoa, between vaccinated and unvaccinated groups, or over time (P>0.05). The BTV-8 vaccine tested in this study did not appear to have any adverse effect on semen quality in rams. [less ▲]

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See detailEffect of an intravenous infusion of lidocaine on cisatracurium-induced neuromuscular block duration: a randomized-controlled trial.
Hans, Grégory ULg; Defresne, Aline ULg; Ki, Bertille et al

in Acta Anaesthesiologica Scandinavica (2010), 54(10), 1192-6

BACKGROUND: Intravenous lidocaine can be used intraoperatively for its analgesic and antihyperalgesic properties but local anaesthetics may also prolong the duration of action of neuromuscular blocking ... [more ▼]

BACKGROUND: Intravenous lidocaine can be used intraoperatively for its analgesic and antihyperalgesic properties but local anaesthetics may also prolong the duration of action of neuromuscular blocking agents. We hypothesized that intravenous lidocaine would prolong the time to recovery of neuromuscular function after cisatracurium. METHODS: Forty-two patients were enrolled in this randomized, double-blind, placebo-controlled study. Before induction, patients were administered either a 1.5 mg/kg bolus of intravenous lidocaine followed by a 2 mg/kg/h infusion or an equal volume of saline. Anaesthesia was induced and maintained using propofol and remifentanil infusions. After loss of consciousness, a 0.15 mg/kg bolus of cisatracurium was administered. No additional cisatracurium injection was allowed. Neuromuscular function was assessed every 20 s using kinemyography. The primary endpoint was the time to spontaneous recovery of a train-of-four (TOF) ratio >/= 0.9. RESULTS: The time to spontaneous recovery of a TOF ratio >/= 0.9 was 94 +/- 15 min in the control group and 98 +/- 16 min in the lidocaine group (P=0.27). CONCLUSIONS: No significant prolongation of spontaneous recovery of a TOF ratio >/= 0.9 after cisatracurium was found in patients receiving intravenous lidocaine. [less ▲]

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See detailEffect of an intubation dose of rocuronium on Spectral Entropy and Bispectral Index (TM) responses to laryngoscopy during propofol anaesthesia
Hans, Pol ULg; Giwer, Jérôme ULg; Brichant, Jean-François ULg et al

in British Journal of Anaesthesia (2006), 97(6), 842-847

Background. The spectral entropy of the electroencephalogram has been proposed to monitor the depth of anaesthesia. State Entropy (SE) reflects the level of hypnosis. Response Entropy (RE), computed from ... [more ▼]

Background. The spectral entropy of the electroencephalogram has been proposed to monitor the depth of anaesthesia. State Entropy (SE) reflects the level of hypnosis. Response Entropy (RE), computed from electroencephalogram and facial muscle activity, reflects the response to nociceptive stimulation. We evaluated the effect of rocuronium on Bispectral Index (TM) (BIS) and entropy responses to laryngoscopy. Methods. A total of 25 patients were anaesthetized with propofol using a target-controlled infusion. At steady state, they randomly received 0.6 mg kg(-1) rocuronium (R) or saline (S). After 3 min, a 20 s laryngoscopy was applied. BIS, RE and SE were recorded continuously and averaged over 1 min during baseline, at steady state, 2 min after R or S administration (R/S+2) and 0, 1, 2 and 3 min after laryngoscopy (L0, L1, L2, L3). Results. At R/S+2, the RE-SE gradient was higher in Group S than in Group R. Laryngoscopy provoked an increase in BIS, RE and SE. Comparing R/S+2 and L0 values in Groups R and S, BIS increased from 43 (6) to 49 (8) and 42 (9) to 51 (15), SE increased from 43 (7) to 50 (8) and 41 (10) to 55 (12), and RE increased from 46 (8) to 54 (9) and 47 (12) to 66 (15), respectively. BIS and SE did not differ between groups. At L0, RE and RE-SE were higher in Group S [66 (15) and 11 (4), respectively] than in Group R [54 (9) and 4 (2), respectively]. Conclusions. Rocuronium alters the RE-SE gradient and the RE and RE-SE responses to laryngoscopy. Muscle relaxation may confound interpretation of entropy monitoring. [less ▲]

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See detailEffect of an oral antioxidant supplementation enriched in (n-3) fatty acids on erythrocyte membrane fluidity in horses.
De Moffarts, Brieuc; Portier, K.; Kirschvink, Nathalie et al

in Proceedings: Congrès de médecine et de chirurgie équine, Genève 2005 (2005)

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See detailThe effect of anaesthetic technique on the incidence of Complex Regional Pain Syndrome after surgical hand fracture: Retrospective approach
Lejeune, Bertrand; Teuwis, Sandrine ULg; Lecoq, Jean-Pierre ULg et al

in Acta Anaesthesiologica Belgica (2008), 59(3), 209

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See detailEffect of androgen treatmen on fear reactions in ewes
Vandenheede, Marc ULg; Bouissou, M. F.

in Hormones & Behavior (1993), 27

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See detailEffect of androgen treatment on fear reactions in ewes.
Bouissou, M. F.; Vandenheede, Marc ULg

Conference (1993)

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See detailEffect of androgen treatment on fear reactions in ewes.
Vandenheede, Marc ULg; Bouissou, M. F.

Conference (1993)

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See detailEffect of Annealing Atmosphere on the Galvanizing Behaviour of a Dual-Phase Steel
Khondker, Rubayat; Mertens, Anne ULg; McDermid, Joe R.

in Materials Science & Engineering : A (2007), 463

The influence of annealing atmosphere oxidation potential on the surface condition of an industrial dual-phase steel alloyed with 2 wt% Mn and 0.3 wt% mo has been studied by galvanizing simulation, with ... [more ▼]

The influence of annealing atmosphere oxidation potential on the surface condition of an industrial dual-phase steel alloyed with 2 wt% Mn and 0.3 wt% mo has been studied by galvanizing simulation, with particular attention towards alloying element segregation, the occurrence of selective oxidation of the surfaces immediately prior to hot-dip galvanizing and their effect on reactive wetting and coating adherence. The surface chemistry and morphology prior to dipping have been analyzed in detail, along with their evolution during annealing, and the coating quality and chemistry were characterized and interpreted in relation to the surface condition prior to dipping. [less ▲]

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See detailEffect of annealing atmosphere on the galvanizing behaviour of a dual-phase steel
Khondker, Rubayat; Mertens, Anne ULg; McDermid, Joe R.

Conference (2006, March)

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See detailEffect of annealing temperatures on properties of sol-gel grown ZnO-ZrO2 films
Ivanova, T.; Harizanova, A.; Koutzarova, T. et al

in Crystal Research And Technology (2010), 45(11), 1154-1160

Mixed ZnO-ZrO2 films have been obtained by sol-gel technology. By using spin coating method, the films were deposited on Si and glass substrates. The influence of thermal annealings (the temperatures vary ... [more ▼]

Mixed ZnO-ZrO2 films have been obtained by sol-gel technology. By using spin coating method, the films were deposited on Si and glass substrates. The influence of thermal annealings (the temperatures vary from 400 degrees C to 750 degrees C) on their structural properties has been studied. The structural behavior has been investigated by the means of XRD and FTIR techniques. The results revealed no presence of mixed oxide phases, the detected crystal phases were related to the hexagonal ZnO and to crystalline ZrO2. The sol-gel ZnO-ZrO2 films showed polycrystalline structure with a certain degree of an amorphous fraction. The optical transmittance reached 91% and it diminished with increasing the annealing temperatures. The optical properties of the sol-gel ZnO-ZrO2 films, deposited on glass substrates are excellent with high transparency and better then those of pure ZrO2 films, obtained at similar technological conditions. (C) 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim [less ▲]

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See detailEFFECT OF ANTI-ANDROGENIC SUBSTANCES ON THE SEXUAL REPRODUCTION OF ROTIFERS : IMPACTS ON BRACHIONUS CALYCIFLORUS MALE FERTILITY
Joaquim-Justo, Célia ULg; Maule, P; Luy, N et al

Conference (2007, August 12)

Many substances released in aquatic ecosystems have been reported to disrupt endocrine systems in vertebrates and invertebrates. In the rotifer Brachionus calyciflorus, studies show that chemicals ... [more ▼]

Many substances released in aquatic ecosystems have been reported to disrupt endocrine systems in vertebrates and invertebrates. In the rotifer Brachionus calyciflorus, studies show that chemicals characterized by a high affinity to the vertebrate androgen receptor depress sexual reproduction at concentrations one to two orders of magnitude below those that affect asexual reproduction. We studied the effect of cyproterone acetate, an anti-androgen of steroid like structure, and of the pesticide fenitrothion, a non steroidal molecular which has a high affinity to the vertebrate androgen receptor, on the sexual reproduction of rotifers. In mating experiments we crossed control females with exposed males and vice versa to determine whether anti-androgens affected males, females or both. Fertilization rate and number of resting eggs produced were affected by both substances tested. With fenitrothion, depression of these endpoints was observed exclusively when males were treated. Cyproterone acetate enhanced production of resting eggs whereas fertilization rate was not significantly affected. Increased production of resting eggs was observed both with treated females and males. Parameters of male fertility were compared in control and in exposed males. We report impacts on spermatozoa numbers, morphology and fertilizing potential, and on male morphology, longevity and mating behaviour for the two substances tested. [less ▲]

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See detailEffect of anti-androgenic substances on the sexual reproduction of the rotifer Brachionus calyciflorus.
Joaquim-Justo, Célia ULg; Snell, T.W

Conference (2006, March 11)

Many substances released in the aquatic environment have been reported to have disruptive effects on endocrine systems of vertebrates and invertebrates. In rotifers, some of these chemicals have been ... [more ▼]

Many substances released in the aquatic environment have been reported to have disruptive effects on endocrine systems of vertebrates and invertebrates. In rotifers, some of these chemicals have been shown to depress sexual reproduction at concentrations one to two orders of magnitude below those that affect asexual reproduction. The anti-androgen flutamide impacts sexual reproduction of rotifers at particularly low concentrations. We studied the effect of different types of putative endocrine disruptors on sexual reproduction of Brachionus calyciflorus. Contaminants tested were cyproterone acetate, an anti-androgen with a steroid-like structure, the pesticides fenitrothion and linuron which both have high affinity to the androgen receptor in vertebrates, although they are nonsteroidal molecules, and lindane a widely used pesticide that binds weakly to the androgen receptor in vertebrates. A series of endpoints relative to different steps of sexual reproduction were monitored to define critical functions affected and to get information on mechanisms of action. Fenitrothion, had the strongest impact on the sexual reproduction of rotifers. We observed a decrease in the proportion of mictic females among the ovigerous individuals and in the production of fertilized eggs per female that was proportional to the dose. The most sensitive parameter was the number of males produced per mictic female which was considerably higher in treated animals even at concentrations 10 times lower than the NOEC of asexual reproduction. [less ▲]

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See detailEffect of antiepileptic drugs and reactive oxygen species on folate receptor 1 (FOLR1)-dependent 5-methyltetrahydrofolate transport.
Opladen, Thomas; Blau, Nenad; RAMAEKERS, Vincent ULg

in Molecular Genetics & Metabolism (2010), 101(1), 48-54

Metabolic breakdown of valproate (VPA), carbamazepine (CBZ) and phenytoin (PHT) by the cytochrome P450 pathway generates toxic drug intermediates and reactive oxygen species (ROS). This mechanism has been ... [more ▼]

Metabolic breakdown of valproate (VPA), carbamazepine (CBZ) and phenytoin (PHT) by the cytochrome P450 pathway generates toxic drug intermediates and reactive oxygen species (ROS). This mechanism has been suspected to play a role in the pathogenesis of secondary cerebral folate deficiency (CFD). Using KB-cell cultures, highly expressing the folate receptor 1 (FOLR1), the effect of antiepileptic drugs (AEDs) and reactive oxygen species (ROS) on the FOLR1 dependent 5-methyltetrahydrofolate (MTHF) uptake was studied. MTHF uptake is time and concentration dependent and shows saturation kinetics. At physiological MTHF concentrations the high-affinity FOLR1 represents the predominant mechanism for cellular incorporation, while at high MTHF concentrations other transport mechanisms participate in folate uptake. Exposure to PHT for more than 8h led to a higher MTHF uptake and decreased cell count, whereas MTHF uptake remained unaltered by VPA and CBZ. However, exposure to superoxide and hydrogen peroxide radicals significantly decreased cellular MTHF uptake. By specific elimination and downregulation of FOLR1 using phosphatidyl-inositol-specific phospholipase C (PIPLC) and siRNA silencing, it was shown that ROS not only inhibited FOLR1 mediated MTHF uptake but also affected all other mechanisms of membrane-mediated MTHF uptake. Generation of ROS with the use of AED might therefore provide an additional explanation for the disturbed folate transfer across the blood-CSF barrier in patients with CFD. [less ▲]

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See detailEffect of aroma compounds on microorganisms
Waché, Y.; Gianotti, A.; Lanciotti, R. et al

Poster (2005, March)

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