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See detailEffect of hypoxia and reoxygenation on gene expression and response to interleukin-1 in cultured articular chondrocytes.
Martin, G.; Andriamanalijaona, R.; Grassel, S. et al

in Arthritis and Rheumatism (2004), 50(11), 3549-60

OBJECTIVE: To determine the effects of hypoxia and reoxygenation on the metabolism of chondrocytes and their response to interleukin-1beta (IL-1beta). The study included activation of hypoxia-inducible ... [more ▼]

OBJECTIVE: To determine the effects of hypoxia and reoxygenation on the metabolism of chondrocytes and their response to interleukin-1beta (IL-1beta). The study included activation of hypoxia-inducible factor 1 (HIF-1), NF-kappaB, and activator protein 1 (AP-1) transcription factors, expression of matrix components and metalloproteases and transforming growth factor beta (TGFbeta) and TGFbeta receptors, and production of nitric oxide (NO) and prostaglandin E(2) (PGE(2)). METHODS: Bovine articular chondrocytes (BACs) were cultured to confluency in either 5% O(2) (hypoxia) or 21% O(2) (normoxia) in media supplemented with 10% fetal calf serum (FCS). BACs were preincubated for 18 hours in media with 1% FCS only and then incubated for 24 hours in the presence of IL-1beta. For reoxygenation experiments, cells were treated in the same way in 5% O(2), except that cultures were transferred to normal atmospheric conditions and used after 4 hours for RNA extraction or after 30 minutes for cytoplasmic or nuclear protein extraction. RESULTS: In hypoxic and reoxygenated chondrocytes, we observed strong DNA binding of HIF-1. IL-1beta-induced DNA binding of NF-kappaB and AP-1 was significantly higher in hypoxic and reoxygenated cultures than in normoxia. Greater activation of the MAPKs was also observed with IL-1beta treatment in hypoxia compared with normoxia. Steady-state levels of type II collagen and aggrecan core protein messenger RNA (mRNA) were decreased by IL-1beta in all instances. Matrix metalloprotease 1 (MMP-1) and MMP-3 mRNA were increased by IL-1beta in normoxia and hypoxia, whereas only MMP-3 mRNA was enhanced in reoxygenated cultures. The MMP-2 mRNA level was not significantly affected by IL-1beta in normoxia or hypoxia, whereas it was enhanced in reoxygenated cultures. MMP-9 mRNA was dramatically decreased by IL-1beta only in low oxygen tension. Tissue inhibitor of metalloproteinases 1 (TIMP-1) message was significantly enhanced by the cytokine in most instances, whereas TIMP-2 message was markedly decreased by IL-1beta in reoxygenated cultures. Stimulation of TGFbeta1 expression by IL-1beta was observed only in normal atmospheric conditions. One of the more striking findings of the study was the greater stimulating effect of IL-1beta on NO production observed in hypoxia, which was much higher than in normoxia, whereas the reverse was observed for IL-1beta-induced PGE(2) production. CONCLUSION: Oxygen level and reoxygenation stress significantly modulate gene expression and the response of articular chondrocytes to cytokines such as IL-1beta. In hypoxic conditions, which mimic the in vivo condition of cartilage, the effects of IL-1beta on both synthesis and degradative processes are significantly different from those in normoxia, conditions that are unlikely encountered by chondrocytes in a normal state. In low oxygen tension, high IL-1beta-induced NO production is associated with a significant decrease in PGE(2) synthesis. These data should influence our concept of the role of oxygen in the pathophysiology of joint disease and may help define the best conditions in which to develop bioartificial cartilage. [less ▲]

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See detailEffect of ice-nucleating bacteria (Pseudomonas syringae Van Hall) on insect susceptibility to sub-zero temperatures
Mignon, Jacques ULg; Haubruge, Eric ULg; Gaspar, Charles ULg

in Journal of Stored Products Research (1998), 34(1), 81-86

Many stored product insect pests are termed "freeze-intolerant" because they cannot survive ice formation in their extracellular body fluid. In grain silos, the progressive decrease in temperature permits ... [more ▼]

Many stored product insect pests are termed "freeze-intolerant" because they cannot survive ice formation in their extracellular body fluid. In grain silos, the progressive decrease in temperature permits the acclimation of insects and enhances their cold tolerance. The objective of this study was to examine the influence of the concentration of the ice-nucleating-active bacterium Pseudomonas syringae (10, 100 and 1000 ppm), temperature and duration of sub-zero exposure on the cold tolerance of the granary weevil Sitophilus granarius (L.) and the saw-toothed grain beetle Oryzaephilus surinamensis (L.). After an application of 1000 ppm of powdered P. syringae to grain, the mortality of S. granarius and O. surinamensis was increased after 24-h exposure to -4 degrees C. Higher mortality was observed after exposure to colder temperatures and a dose-response relationship was evident. At near-zero (-4 to 0 degrees C) negative temperatures, no dose response was observed, and the mortality in treated grain was the same as that in untreated grain. (C) 1998 Elsevier Science Ltd. All rights reserved. [less ▲]

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See detailEffect Of Imbalance Between Energy And Nitrogen Supplies On Microbial Protein Synthesis And Nitrogen Metabolism In Growing Double-Muscled Belgian Blue Bulls
Valkeners, Damien; Thewis, André ULg; Piron, Fabien ULg et al

in Journal of Animal Science (2004), 82(6), 1818-1825

Six double-muscled Belgian Blue bulls (initial weight: 345 ± 16 kg) with cannulas in the rumen and proximal duodenum were used in two juxtaposed 3 × 3 Latin squares to study the effect of a lack of ... [more ▼]

Six double-muscled Belgian Blue bulls (initial weight: 345 ± 16 kg) with cannulas in the rumen and proximal duodenum were used in two juxtaposed 3 × 3 Latin squares to study the effect of a lack of synchronization between energy and N in the rumen on microbial protein synthesis and N metabolism by giving the same diet according to three different feeding patterns. The feed ingredients of the diet were separated into two groups supplying the same amount of fermentable OM (FOM), but characterized by different levels of ruminally degradableN (RDN). The first group primarily provided energy for the ruminal microbes (14.6 g of RDN/kg of FOM), and the second provided N (33.3 g of RDN/kg of FOM). These two groups were fed to the bulls simultaneously or alternately with the aim of creating three different time periods of imbalance (0, 12, or 24 h) between energy and N supplies in the rumen. The introduction of imbalance affected neither microbial-N flow at the duodenum (P = 0.65) nor efficiency of growth (P = 0.69), but decreased (P = 0.016) the NDF degradation in the rumen 12.2% for a 12-h period of imbalance. N retention was not affected by imbalance (P = 0.53) and reached 57.8, 58.5, and 54.7 g/d, respectively, for 0-, 12- and 24-h imbalance. It seems that the introduction of an imbalance of 12 or 24 h between energy and N supplies for the ruminal microbes by altering the feeding pattern of the same diet does not negatively influence microbial protein synthesis or N retention by the animal. Nitrogen recycling in the rumen plays a major role in regulating the amount of ruminally availableNand allows for continuous synchronization of N- and energy-yielding substrates for the microorganisms in the rumen. Therefore, a lack of synchronization in the diet between the energy and N supplies for the ruminal microbes is not detrimental to their growth or for the animal as long as the nutrient supply is balanced on a 48-h basis. Thus, these dietary feeding patterns may be used under practical feeding conditions with minimal effect on the performance of ruminant animals. [less ▲]

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See detailEffect of imbalance between energy and nitrogen supplies on microbial protein synthesis in growing double-muscled Belgian Blue bulls.
Valkeners, Damien; Beckers, Yves ULg; Piron, Fabien ULg et al

in Proceeding of the British Society of Animal Science 2003 (2003)

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See detailEffect of improved feeding and housing, Friesian blood level and parity on milk production of Ankole x Friesian cows
Kibwana, Denise Katungu; Makumyaviri, A.M.; Hornick, Jean-Luc ULg

in Pakistan Veterinary Journal (in press)

In North Kivu, D.R. Congo, cattle are raised in extensive systems based on local breeds and crossbreds. This farming method affects the performance of dairy animals and mortgages the level of milk ... [more ▼]

In North Kivu, D.R. Congo, cattle are raised in extensive systems based on local breeds and crossbreds. This farming method affects the performance of dairy animals and mortgages the level of milk consumption in population. An improved farming system, including feed supplementation, Friesian cross-breeding and the sheltering of animals in stalls at night, was studied to evaluate its effect on milk production by Ankole x Friesian crossbreds. The study was performed in Beni, D.R. Congo, on two groups of 15 animals (control group-CoG and improved group-IG), homogeneous according to Friesian blood and parity levels. The improved system, Friesian blood and parity levels increased significantly milk production (P<0.001). Farming system increased milk production by 1.6 l/d (5.2 l/d vs 6.8 l/d respectively in CoG and IG). Milk production ranged from 5.3±0.04 l/d to 6.6±0.02 l/d and from 4.9±0.02 l/d to 7.2±0.02 l/d according, respectively, to Friesian blood level and parity. A significant interaction was observed between farming system and Friesian blood level, and between farming system and parity (P<0.001). The two groups were characterized by a fair adaptation to forage availability associated to climatic factors. The study showed the importance of genetic and environmental factors on the milk production of crossbred dairy cows of the region. [less ▲]

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See detailEffect of improved parboiling methods on the physical and cooked grain characteristics of rice varieties in Benin
Fofana, Mamadou; Wanvoeke, Jonas; Manful, John et al

in International Food Research Journal [=IFRJ] (2011), 18

The efficiency of traditional, intermediate and improved parboilers was compared through their effects on certain physical and cooking quality traits. Two varieties (NERICA 4 and Gambiaka) commonly ... [more ▼]

The efficiency of traditional, intermediate and improved parboilers was compared through their effects on certain physical and cooking quality traits. Two varieties (NERICA 4 and Gambiaka) commonly cultivated and consumed in Benin were used. Results showed that the traditional parboiler had the highest level of heat-damaged grains (90%) with the improved equipment having the least (17%). The improved and intermediate parboiling technology produced grains of comparable hardness (4 kg and 6 kg, respectively, for Gambiaka and NERICA 4) while the traditional method resulted in a sample with the least hardness for both Gambiaka (4 kg) and NERICA 4 (3 kg). The improved method and the intermediate technology using wooden sticks at the bottom of the vessel had higher water uptake (2.97 ml/grain) and grain swelling ratios (5.41) as compared to the traditional and intermediate methods using a container with a perforated bottom. [less ▲]

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See detailEffect of imputing markers from a low-density chip on the reliability of genomic breeding values in Holstein populations.
Dassonneville, Romain; Brondum, R. F.; Druet, Tom ULg et al

in Journal of Dairy Science (2011), 94(7), 3679-86

The purpose of this study was to investigate the imputation error and loss of reliability of direct genomic values (DGV) or genomically enhanced breeding values (GEBV) when using genotypes imputed from a ... [more ▼]

The purpose of this study was to investigate the imputation error and loss of reliability of direct genomic values (DGV) or genomically enhanced breeding values (GEBV) when using genotypes imputed from a 3,000-marker single nucleotide polymorphism (SNP) panel to a 50,000-marker SNP panel. Data consisted of genotypes of 15,966 European Holstein bulls from the combined EuroGenomics reference population. Genotypes with the low-density chip were created by erasing markers from 50,000-marker data. The studies were performed in the Nordic countries (Denmark, Finland, and Sweden) using a BLUP model for prediction of DGV and in France using a genomic marker-assisted selection approach for prediction of GEBV. Imputation in both studies was done using a combination of the DAGPHASE 1.1 and Beagle 2.1.3 software. Traits considered were protein yield, fertility, somatic cell count, and udder depth. Imputation of missing markers and prediction of breeding values were performed using 2 different reference populations in each country: either a national reference population or a combined EuroGenomics reference population. Validation for accuracy of imputation and genomic prediction was done based on national test data. Mean imputation error rates when using national reference animals was 5.5 and 3.9% in the Nordic countries and France, respectively, whereas imputation based on the EuroGenomics reference data set gave mean error rates of 4.0 and 2.1%, respectively. Prediction of GEBV based on genotypes imputed with a national reference data set gave an absolute loss of 0.05 in mean reliability of GEBV in the French study, whereas a loss of 0.03 was obtained for reliability of DGV in the Nordic study. When genotypes were imputed using the EuroGenomics reference, a loss of 0.02 in mean reliability of GEBV was detected in the French study, and a loss of 0.06 was observed for the mean reliability of DGV in the Nordic study. Consequently, the reliability of DGV using the imputed SNP data was 0.38 based on national reference data, and 0.48 based on EuroGenomics reference data in the Nordic validation, and the reliability of GEBV using the imputed SNP data was 0.41 based on national reference data, and 0.44 based on EuroGenomics reference data in the French validation. [less ▲]

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See detailEffect of inclusions and grain boundary segregations on the oxidation-corrosion behavior of rolls alloys by electrochemical impedance spectroscop
Perez, F. J.; Duday, D.; Hierro, M. P. et al

in Proceedings of the MSMF-3 International Conference (2001)

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See detailEffect of increased CO2 in the second half of incubation on embryonic growth and on the hatching process
Everaert, Nadia ULg; Bruggeman, V.; De Smit, L. et al

Conference (2006)

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See detailEffect of increasing speed and incline on ventilation in thoroughbred horses exercising on a treadmill
Art, Tatiana ULg; Anderson, L.; Roberts, c et al

in Archives Internationales de Physiologie et de Biochimie (1989), 97(5), 85

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See detailEffect of incubation temperature and relative humidity on lesion diameter of Botrytis cinerea Pers. and Penicillium expansum Link. on apple fruits.
Lahlali, R.; Friel, D.; Serrhini, M Najib et al

in Communications in agricultural and applied biological sciences (2006), 71(3 Pt B),

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See detailEffect of indomethacin on the metabolic and hormonal response to a standardized breakfast in normal subjects.
Luyckx, A. S.; Guerten, D.; Scheen, André ULg et al

in Acta Diabetologica Latina (1981), 18(3), 259-66

We have investigated the influence of a single oral administration of indomethacin on blood glucose, plasma free fatty acids (FFA), alpha-amino-nitrogen, insulin and glucagon concentrations in young ... [more ▼]

We have investigated the influence of a single oral administration of indomethacin on blood glucose, plasma free fatty acids (FFA), alpha-amino-nitrogen, insulin and glucagon concentrations in young healthy subjects. Two groups of 6 subjects were studied, the first received a standardized 500 kcal mixed meal without any previous drug administration (controls) whereas the second group received 50 mg indomethacin 2 h before ingesting an identical meal. Plasma indomethacin concentration reached its maximum (2.36 +/- 0.36 micro g/ml) 15 min after administration and declined to 0.45 +/- 0.04 micro g/ml after 2 h. Indomethacin ingestion was followed by a significant increase in blood glucose and plasma FFA reaching their maximum value at 45 min and returning to basal levels at 120 min. No simultaneous changes in plasma alpha-amino-nitrogen, insulin or glucagon levels were detected during this period. The meal was followed by a rise in blood glucose and plasma insulin as well as by a decrease in plasma FFA concentration. No significant differences were detected between the controls and the subjects receiving indomethacin. In controls, the meal was followed by a rise in plasma alpha-amino-nitrogen and a modest although significant increase in glucagon levels. In indomethacin-treated subjects, the increment of alpha-amino-nitrogen was less marked and the increase in plasma glucagon was not observed. Thus, indomethacin by itself can exert several metabolic effects; however, it does not deteriorate the blood glucose or insulin profile after a regular meal. The present work is the first to demonstrate that an inhibitor of prostaglandin synthesis inhibits the plasma glucagon rise occurring after a physiological stimulus such as a normal meal. On the basis of previous in vitro experiments, we suggest that this effect results from an inhibition of glucagon secretion by the PG synthesis inhibitor. [less ▲]

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See detailEffect of Inducers and Pcbs on the Cytochrome P450 Enzymes in Cultured Quail Hepatocytes
Roelandt, L.; Dubois, Maryline; Todaro, Anna ULg et al

in Ecotoxicology & Environmental Safety (1995), 31(2), 158-63

Hepatocytes isolated from fetal quail livers (Coturnix coturnix japonica) were cultured in vitro. Their capacity to metabolize drugs and xenobiotics was explored with typical cytochrome P450 substrates ... [more ▼]

Hepatocytes isolated from fetal quail livers (Coturnix coturnix japonica) were cultured in vitro. Their capacity to metabolize drugs and xenobiotics was explored with typical cytochrome P450 substrates: ethoxycoumarin (known to be metabolized by several P450s), ethoxyresorufin (essentially dealkylated by P450IA1), and testosterone (specifically hydroxylated at several positions by several P450s). The cells could be kept metabolically active in culture for at least 4 days. Their drug-metabolizing activities were inducible by the usual P450 inducers, like phenobarbital and benzanthracene, but also by Aroclor 1254, a PCB mixture. The results obtained indicate that this experimental model could certainly be very helpful in ecotoxicological studies. [less ▲]

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See detailEffect of induction of late embryonic mortality on plasma profiles of pregnancy associated glycoprotein 1 in heifers
Szenci, Otto; Beckers, Jean-François ULg; Sulon, Joseph ULg et al

in Veterinary Journal (2003), 165(3), 307-313

Inoculation with Actinomyces pyogenes and administration of prostaglandin (PG) F-2alpha were used to induce late embryonic mortality (LEM) in heifers (n = 8) on Days 30-38 of pregnancy in order to compare ... [more ▼]

Inoculation with Actinomyces pyogenes and administration of prostaglandin (PG) F-2alpha were used to induce late embryonic mortality (LEM) in heifers (n = 8) on Days 30-38 of pregnancy in order to compare the profile for bovine pregnancy associated glycoprotein 1 (PAG1), progesterone and 15-keto-13,14-dihydro-PGF(2alpha) (PGFM). Two pregnant heifers were used as controls. Inoculation into the uterine body caused LEM, as established by ultrasonography in each heifer within 24 h of treatment. When the inoculum was injected into the first part of the cervix, LEM occurred in one of two heifers (Heifer A) between 48 and 72 h after treatment. Similarly, PGF(2alpha) treatment caused LEM in three of four heifers. In six of eight heifers, PAG1 started to decrease steadily when it was accompanied by the subsequent death of the embryo. Inoculation through the cervix caused luteolysis in three of four heifers within 6-10 days after induction. After induction of LEM, PGFM concentrations showed a two to 3.8 fold increase in three of four heifers during the following six days, and from that time changed within normal ranges. The results of this study indicate that a PAG1 assay may provide an alternative method to ultrasonography for determining LEM in the cow. [less ▲]

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See detailEffect of infliximab treatment on colonic mucosal gene expression profiles in patients with inflammatory bowel disease
Arijs, I.; Quintens, R.; Van Lommel, L. et al

in Gut (2008), 57(Suppl II), 39

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See detailEffect of inhaled fluticasone on airway reactivity and 8-Iso-PGF2 in bronchoalveolar lavage fluid of cats
Kirschvink, N.; Leemans, J.; Delvaux, François ULg et al

in 5th China - France Pharmacological Joint Symposium - Beijing - France - Octobre 2004 (2004, October)

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