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See detailDetermination of the lower critical field Hc1(T) in FeSe single crystals by magnetization measurements
Abdel-Hafiez, M; Vasiliev, A.N.; Chareev, D.A. et al

in Physica C: Superconductivity (2014), 503

In a recent work, Abdel-Hafiez et al. we have determined the temperature dependence of the lower critical field Hc1(T) of a FeSe single crystal under static magnetic fields H parallel to the ... [more ▼]

In a recent work, Abdel-Hafiez et al. we have determined the temperature dependence of the lower critical field Hc1(T) of a FeSe single crystal under static magnetic fields H parallel to the crystallographic c axis. The temperature dependence of the first vortex penetration field has been experimentally obtained by two independent methods and the corresponding Hc1(T) was deduced by taking into account demagnetization factors. In general, the first vortex penetration field may not reflect the true Hc1(T) due to the presence of surface barriers. In this work we show that magnetic hysteresis loops are very symmetric close to the critical temperature Tc = 9 K evidencing the absence of surface barriers and thus validating the previously reported determination of Hc1(T) and the main observations that the superconducting energy gap in FeSe is nodeless. [less ▲]

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See detailDetermination of the magnetic penetration depth in a superconducting Pb film
Brisbois, Jérémy ULg

Conference (2014, May 01)

By means of scanning Hall probe microscopy technique we accurately map the magnetic field pattern produced by Meissner screening currents in a thin superconducting Pb stripe. The obtained field profile ... [more ▼]

By means of scanning Hall probe microscopy technique we accurately map the magnetic field pattern produced by Meissner screening currents in a thin superconducting Pb stripe. The obtained field profile allows us to quantitatively estimate the Pearl length Λ without the need of pre-calibrating the Hall sensor. This fact contrasts with the information acquired through the spatial field dependence of an individual flux quantum where the scanning height and the magnetic penetration depth combine in a single inseparable parameter. The derived London penetration depth λL coincides with the values previously reported for bulk Pb once the kinetic suppression of the order parameter is properly taken into account. [less ▲]

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See detailDetermination of the magnetic penetration depth in a superconducting Pb film
Brisbois, Jérémy ULg; Raes, Bart; Van de Vondel, Joris et al

in Journal of Applied Physics (2014), 115(10), 103906

By means of scanning Hall probe microscopy technique we accurately map the magnetic field pattern produced by Meissner screening currents in a thin superconducting Pb stripe. The obtained field profile ... [more ▼]

By means of scanning Hall probe microscopy technique we accurately map the magnetic field pattern produced by Meissner screening currents in a thin superconducting Pb stripe. The obtained field profile allows us to quantitatively estimate the Pearl length Λ without the need of pre-calibrating the Hall sensor. This fact contrasts with the information acquired through the spatial field dependence of an individual flux quantum where the scanning height and the magnetic penetration depth combine in a single inseparable parameter. The derived London penetration depth λL coincides with the values previously reported for bulk Pb once the kinetic suppression of the order parameter is properly taken into account. [less ▲]

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See detailDetermination of the mass of nearby stars from astrometric microlensing observations
Dib, S.; Claeskens, Jean-François ULg; Surdej, Jean ULg

in Surdej, Jean; Swings, J.-P.; Caro, D. (Eds.) et al Proceedings of the LIAC “From optical to millimetric interferometry, scientific and technological challenges” (2001)

The possibility of determining, with a precision of 10%, the mass of bright, nearby stars by means of astrometric microlensing observations is investigated in the context of the future ground-based and ... [more ▼]

The possibility of determining, with a precision of 10%, the mass of bright, nearby stars by means of astrometric microlensing observations is investigated in the context of the future ground-based and spaceborne high precision astrometric instruments, such as the VLT interferometer, GAIA and SIM. [less ▲]

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See detailDetermination Of The Methionine Requirement Of Finishing Double-Muscled Belgian Blue Bulls With A Three-Step Method
Froidmont, Eric; Beckers, Yves ULg; Thewis, André ULg

in Canadian Journal of Animal Science (2002), 82(1), 95-102

A three-step technique was used to determine total amino acids (AA) and the first limiting AA requirements in finishing double-muscled Belgian Blue (dmBB) bulls. In a first experiment, three dmBB bulls ... [more ▼]

A three-step technique was used to determine total amino acids (AA) and the first limiting AA requirements in finishing double-muscled Belgian Blue (dmBB) bulls. In a first experiment, three dmBB bulls (505 ± 21 kg) received a low metabolizable protein diet containing 25% meadow hay and 75% concentrate. Net energy supply was adequate for maximizing daily gains because of continuous infusion of dextrose into the duodenum. The intestinal apparent disappearance of essential AA (EAA) averaged 70.8% and was the lowest for histidine (61.3%) and the highest for arginine (79.9%). In a second experiment, four dmBB bulls (517 ± 16 kg) received the same diet supplemented with duodenal infusion of dextrose and four doses of Na-caseinate (17, 33, 50 and 66% of metabolizable dietary AA) in a 4 × 4 Latin square design. Nitrogen retention for the basal diet alone and the four increasing supplements of Na-caseinate averaged 61, 64, 74, 75 and 78 g d–1, respectively. A supply of 720 g d–1 of metabolizable AA was defined as optimising the N utilization for animal growth. Based on patterns of plasma concentrations, methionine and phenylalanine were probably the limiting AA. In a third experiment, five dmBB bulls (513 ± 60 kg) fed the basal diet received duodenal infusion of dextrose and AA, equivalent to the second dose in exp. 2 except for the supply of metabolizable methionine (12.8, 15.1, 17.6, 20.1, 22.6 and 25.1 g d–1) that varied in a 6 × 6 Latin square design with one missing animal. On the basis of N retention, the metabolizable methionine requirement was estimated to 22.8 g d–1 and corresponded to 360 mg of metabolizable methionine per gram of N retained. [less ▲]

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See detailDetermination Of The Methionine Requirement Of Growing Double-Muscled Belgian Blue Bulls With A Three-Step Method
Froidmont, Eric; Beckers, Yves ULg; Thewis, André ULg

in Journal of Animal Science (2000), 78(1), 233-241

The three-step technique was used to determine the requirements of total amino acids (TAA) and the first-limiting amino acid (AA) in growing double- muscled Belgian Blue bulls (BBb). In Exp. 1, three ... [more ▼]

The three-step technique was used to determine the requirements of total amino acids (TAA) and the first-limiting amino acid (AA) in growing double- muscled Belgian Blue bulls (BBb). In Exp. 1, three double-muscled BBb weighing initially 306 ± 28 kg received a basal diet consisting of 30% meadow hay and 70% concentrate that was poor in digestible protein but had adequate NE because of continuous infusion of dextrose into the duodenum. The intestinal apparent digestibility of essential AA (EAA) was defined according to their duodenal and ileal flows. It averaged 72% but varied between 60% for Met and 79% for Arg. In Exp. 2, five double-muscled BBb (334 ± 22 kg) received the same diet supplemented with duodenal infusions of dextrose and four doses of Na-caseinate (28, 56, 84, and 112% of intestinal digestible dietary AA) in a 4 × 4 Latin square design with one additional animal. Nitrogen retention for the basal diet alone and the four increasing supplements of Na-caseinate reached 49, 61, 70, 80, and 86 g/d, respectively. Nitrogen utilization improved from 34.3% without Na-caseinate supplementation to a maximum of 40.6%, with the third dose supplying 788 g/d of apparently digestible AA. Based on patterns of plasma concentrations, Met, Phe, and Arg were probably the limiting AA when animals optimized N utilization. In Exp. 3, six double-muscled BBb (315 ± 25 kg) fed the basal diet received duodenal infusions of dextrose and AA, equivalent to the third dose in Exp. 2, except for digestible Met (9.3, 14.4, 18.4, 22.4, 26.4, and 30.4 g/d) in a 6 × 6 Latin square design. The Met requirement was close to 26.4 g/d on the basis of N retention. [less ▲]

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See detailDetermination Of The Minimal Fusion Peptide Of Bovine Leukemia Virus Gp30
Lorin, A.; Lins, Laurence ULg; Stroobant, V. et al

in Biochemical and Biophysical Research Communications (2007), 355(3), 649-53

In this study, we determined the minimal N-terminal fusion peptide of the gp30 of the bovine leukemia virus on the basis of the tilted peptide theory. We first used molecular modelling to predict that the ... [more ▼]

In this study, we determined the minimal N-terminal fusion peptide of the gp30 of the bovine leukemia virus on the basis of the tilted peptide theory. We first used molecular modelling to predict that the gp30 minimal fusion peptide corresponds to the 15 first residues. Liposome lipid-mixing and leakage assays confirmed that the 15-residue long peptide induces fusion in vitro and that it is the shortest peptide inducing optimal fusion since longer peptides destabilize liposomes to the same extent but not shorter ones. The 15-residue long peptide can thus be considered as the minimal fusion peptide. The effect of mutations reported in the literature was also investigated. Interestingly, mutations related to glycoproteins unable to induce syncytia in cell-cell fusion assays correspond to peptides predicted as non-tilted. The relationship between obliquity and fusogenicity was also confirmed in vitro for one tilted and one non-tilted mutant peptide. [less ▲]

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See detailDetermination Of The Minimal Fusion Peptide Of Hiv, Siv And Blv Fusion Glycoproteins
Lorin, A.; Charloteaux, Benoît ULg; Lins, Laurence ULg et al

in Peptides For Youth - the Proceedings of the 20th American Peptidesymposium (2009), 611

The entry of enveloped viruses into target cells requires the fusion between the viral envelope and the target cell membrane. In the case of many viruses like HIV, SIV and BLV, the fusion is mediated by ... [more ▼]

The entry of enveloped viruses into target cells requires the fusion between the viral envelope and the target cell membrane. In the case of many viruses like HIV, SIV and BLV, the fusion is mediated by class 1 fusion glycoproteins located on the viral envelope. These fusion glycoproteins contain a region at their N-terminal extremity called the “fusion peptide”, which interact with the target membrane. Many mutagenesis studies showed that this region is required for mediating membrane fusion [1]. Moreover, synthetic peptides corresponding to the fusion peptide of many glycoproteins induce membrane fusion in vitro. Despite the large number of studies on synthetic fusion peptides, the region necessary and sufficient to induce optimal membrane fusion is not known. To determine this minimal fusion peptide, we used the “tilted peptide” theory. According to this theory, a helical peptide inserting obliquely into membranes induces fusion [2]. Moreover, the more tilted the peptide is, the more important the fusion is. Then, we postulate that the minimal fusion peptide corresponds to the shortest helical fragment able to insert into the membrane with an angle close to 45°. This peptide was predicted using the IMPALA algorithm, which allow to predict peptide-membrane interactions [3]. Fusogenicity of this peptide was then assessed in liposome lipid-mixing and leakage assays and compared to the fusogenicity of smaller and longer peptides to check the validity of the prediction. This methodology was used to determine successfully the minimal fusion peptide of three viruses, HIV, SIV and BLV. [less ▲]

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See detailDetermination of the molecular players of adaptation to anti-angiogenic therapy in breast cancer by quantitative proteomic and high molecular MALDI Imaging.
Cimino, Jonathan ULg; Sounni, Nor Eddine ULg; Calligaris, David ULg et al

Poster (2012, October 13)

Breast carcinoma is the most common and second leading cause of cancer mortality in women. The recognition of the “angiogenic switch” as a rate-limiting secondary step in tumorigenesis led to extensive ... [more ▼]

Breast carcinoma is the most common and second leading cause of cancer mortality in women. The recognition of the “angiogenic switch” as a rate-limiting secondary step in tumorigenesis led to extensive pre-clinical researches on angiogenesis and finally the approval of VEGF-neutralizing antibodies (bevacizumab) and VEGF receptor tyrosine kinase inhibitors (RTKs:Sunitinib). The Sunitinib has been used clinically in patients with breast cancer refractory to other therapeutic agents. Unfortunately, like the cytotoxic therapies, these drugs do not produce lasting effects and resistance to treatment appeared clinically. Questions have emerged about the failure of anti-angiogenic therapy in clinic and the limitations of predictive preclinical models, and also about the molecular assessment of all stages of tumor adaptation and me<x>tastatic disease. To this end, we applied quantitative proteomics and imaging mass spectrometry tools to visualize and study the profiles of proteins and small molecules associated with tumor treated or not with Sunitinib using a novel preclinical model of breast carcinoma cells. In this project, we first developed a reproducible model of resistance to Sunitinib of human triple negative breast cancer MDA-MB-231 cells expressing luciferase gene. Cells were subcutaneously injected into mice RAG1-/- and divided into four experimental groups including, control mice treated with vehicle or Sunitinib for 30 days and sacrificed 1 days after treatment withdrawal or when tumor reached a volume of 300 mm3. In the second step. Tumors were analyzed using a nanoAcquity UPLC Synapt TM HDMS TM G1 (Waters, Manchester,UK) and Mass Spectrometry Imaging. For quantitative proteomic analyses of tumors, a bioinformatics analysis was used with the Protein lynx global server 2.2.5 software. Imaging mass spectrometry was performed on tissue sections of tumors and organs subsequently colonized by me<x>tastases. Matrix sublimation was used to coat tumor sections (14 µm-tick) with 1.5 Diaminonaphthalene for lipids analysis and Sinapinic acid for entire proteins analysis. Ion cartographies were recorded with a Solarix 9.4T FTMS instrument for lipids and with an Ultraflex II TOF-TOF instrument for entire proteins (Bruker Daltonics, Germany) with a spatial resolution of 100 µm. Global protemic revealed different protein profiles between tumor treated or not with Sunitinib. The Mass Spectrometry Imaging detected differences in intensity and location of some proteins and lipids are also associated with some histological features including inflammatory, necrotic and angiogenic areas. Bioinformatics analysis will be applied to ensure the integration of all data in order to provide the basis for identifying molecular pathways activated during the acquisition of refractoriness to drug treatments. [less ▲]

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See detailDetermination of the optimal forging conditions of a Cr-Mo-V high alloy steel through a microstructural, thermophysical and mechanical study
Bouffioux, Chantal ULg; Carton, Marc ULg; Lecomte-Beckers, Jacqueline ULg et al

in Banabic, D. (Ed.) Proceedings of the 8th ESAFORM Conference on Material Forming (Vol. 1) (2005)

The forging process of cylinders requires the knowledge of material recrystallisation conditions to prevent crack appearance. Due to the continuous recrystallisation during forging, very large ... [more ▼]

The forging process of cylinders requires the knowledge of material recrystallisation conditions to prevent crack appearance. Due to the continuous recrystallisation during forging, very large deformations can be applied as the generated dislocations do not yield to hardening but to recrystallisation phenomenon. This paper summarizes the data identification of a FEM recrystallisation model and defines the optimal forging process for one roll. [less ▲]

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See detailDetermination of the performance characteristics of enzyme immunoassays using the photon era automatic analyszer
Chapelle, Jean-Paul ULg; El Allaf, M.

in Annals of Clinical Biochemistry (1987), 24(suppl. 2), 116

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See detailDetermination of the pole orientation of an asteroid - The amplitude-aspect relation revisited
Pospieszalska-Surdej, Anna ULg; Surdej, Jean ULg

in Astronomy and Astrophysics (1985), 149

Near the opposition of a minor planet the geometrical approximation essentially constitutes a good representation of the Hapke-Irvine relation for describing the scattering properties of a surface layer ... [more ▼]

Near the opposition of a minor planet the geometrical approximation essentially constitutes a good representation of the Hapke-Irvine relation for describing the scattering properties of a surface layer, and the authors show that the normalized light curve of a three-axes ellipsoid model reduces to a straight line whose slope depends only on the aspect angle A and on the semi-axes ratios a/b, b/c of the ellipsoid. A set of non-linear equations is then solved by a least squares method in order to derive the four unknown parameters lambda[SUB]0[/SUB], beta[SUB]0[/SUB] (ecliptic coordinates of the pole) and a/b, b/c. The authors have applied this technique to published observations of two asteroids: For (624) Hektor two possible solutions are found; and for the case of (44) Nysa, they show that additional observations are needed in order to derive a self-consistent pole orientation. [less ▲]

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See detailDetermination of the redshift of an invisible lens
Jean, C.; Surdej, Jean ULg

Conference (1998)

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See detailDetermination of the relationship between foam morphology and electrical conductivity of polymer/carbon nanotube nanocomposite foams
Tran, Minh Phuong ULg

Doctoral thesis (2014)

The lightweight of porous nanocomposites makes them attractive materials for various applications such as thermal and sound barriers, shock absorbers, insulation, packaging, and their porous structure is ... [more ▼]

The lightweight of porous nanocomposites makes them attractive materials for various applications such as thermal and sound barriers, shock absorbers, insulation, packaging, and their porous structure is very interesting in bone tissue engineering. Moreover, the incorporation of appropriate carbonaceous nanoparticles into polymeric foams contributes to the reinforcement of their mechanical performances but also renders them electrically conductive, consequently extending their potential interest in electromagnetic shielding (EMI) and electrostatic discharge (ESD) applications for instance. In this PhD thesis, we aim at designing various polymeric foams containing a conductive nanofiller (carbon nanotubes) and to identify the main morphological parameters (pore size, cell density, cell wall thickness,…) that affect and govern the final properties of the foams. In this work, the electrical conductivity of the foams is the main property investigated because it is governing their performances as materials for EMI absorbers, the main application targeted in this work. These important morphology/electrical conductivity relationships would indeed be very useful to guide the foam development towards the material with the best performances for the targeted applications. Two different foaming methods are used in this work: (i) the supercritical CO2 (scCO2) foaming technology and (ii) the freeze-drying process. The first technique enables to produce isotropic foams with spherical closed cells structures and the second one, oriented anisotropic foams with cylindrical open cells. The variation of the foaming parameters allows preparing foams with a large panel of morphologies required for the establishment of the structure/properties relationships. In parallel to this main objective, an improvement of the overall conductive performances of the nanocomposites foams is also investigated through the optimization of the foam morphology and the content in conductive nanofillers. [less ▲]

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See detailDetermination of the respective contributions of energy-dissipating athways to mitochondrial respiration : The ADP/O method
Sluse, Francis ULg; Jarmuszkiewicz, W.; Almeida, A. et al

in Moller, I. M.; Gardestrom, P.; Glimelius, K. (Eds.) et al Plant mitochondria : from gene to function (1998)

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See detailDetermination of the respective contributions of the cytochrome and alternative oxidase pathway in Acanthamoeba catellanii
Jarmuszkiewicz, W.; Sluse-Goffart, C.; Hryniewiecka, L. et al

in Westerhoff, H.; Snoep, J.; Sluse, Francis (Eds.) et al Biothermokinetics of the living cell (1996)

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See detailDetermination of the response factors of several desulfoglucosinolates used for quantitative analysis of Brassicaceae
Wathelet, Jean-Paul ULg; Iori, R.; Mabon, N. et al

Poster (2001)

Quantitative determination of total and individual glucosinolate contents in Brassica samples (rapeseed/canola, broccoli, cabbage, mustard…) is generally realised according to the reference method ISO ... [more ▼]

Quantitative determination of total and individual glucosinolate contents in Brassica samples (rapeseed/canola, broccoli, cabbage, mustard…) is generally realised according to the reference method ISO 9167-1. After extraction, glucosinolates are purified, then desulfated by Helix pomatia sulfatase. The desulfo-glucosinolates (DSGSL) formed are then separated by reverse phase chromatography associated with UV detection (229 nm). As each compound can possess a very different molar extinction coefficient, it is important - on a quantitative point of view - to define accurately relative response factors. The response factors actually used in the official method have been determined experimentally through indirect methods and have been fixed, some years ago, by consensus between the various laboratories who took part in a ring test. They may need to be revised and calculated for a broader panel of glucosinolates. [less ▲]

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See detailDetermination of the ruminant origin of bone particles using fluorescence in situ hybridization (FISH).
Lecrenier, Marie-Caroline ULg; Ledoux, Quentin; Berben, Gilbert et al

in Scientific reports (2014), 4

Molecular biology techniques such as PCR constitute powerful tools for the determination of the taxonomic origin of bones. DNA degradation and contamination by exogenous DNA, however, jeopardise bone ... [more ▼]

Molecular biology techniques such as PCR constitute powerful tools for the determination of the taxonomic origin of bones. DNA degradation and contamination by exogenous DNA, however, jeopardise bone identification. Despite the vast array of techniques used to decontaminate bone fragments, the isolation and determination of bone DNA content are still problematic. Within the framework of the eradication of transmissible spongiform encephalopathies (including BSE, commonly known as "mad cow disease"), a fluorescence in situ hybridization (FISH) protocol was developed. Results from the described study showed that this method can be applied directly to bones without a demineralisation step and that it allows the identification of bovine and ruminant bones even after severe processing. The results also showed that the method is independent of exogenous contamination and that it is therefore entirely appropriate for this application. [less ▲]

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