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See detailDevelopment and validation of a short Italian UPPS-P Impulsive Behavior Scale
D'Orta, Isabella; Burnay, Jonathan ULg; Aiello, Daniela et al

in Addictive Behaviors Reports (2015), 2

Impulsivity is a multidimensional construct that plays a prominent role in the development, maintenance, and relapse of addictive disorders. The UPPS-P model of impulsivity, which distinguishes between ... [more ▼]

Impulsivity is a multidimensional construct that plays a prominent role in the development, maintenance, and relapse of addictive disorders. The UPPS-P model of impulsivity, which distinguishes between five impulsivity components (positive urgency, negative urgency, lack of perseverance, lack of premeditation, sensation seeking), has been increasingly investigated during the last decade in relation to addictive and risky behaviors. Unfortunately, it currently lacks a validated scale that allows Italian researchers and clinicians to measure impulsivity based on the UPPS-P model. The current study fills this gap by testing the psychometric properties of a short 20-item Italian scale used to assess the five dimensions of the UPPS-P model in 188 volunteer participants from the community. Confirmatory factor analysis supported a model of five distinct, but interrelated, impulsivity components. The results indicated good internal consistency (Cronbach's α ranges from .73 to .84). Construct validity was evidenced by specific relations with measures of addictive behaviors and depressive symptoms. On the whole, this study demonstrated that the Italian short UPPS-P has good psychometric properties. [less ▲]

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See detailDevelopment and validation of a specific radioimmunoassay for equine osteocalcin
Carstanjen, Bianca; Sulon, Joseph ULg; Banga-Mboko, H. et al

in Domestic Animal Endocrinology (2003), 24(1), 31-41

This study describes for the first time the development and validation of a sensitive and specific radioimmunoassay (RIA) for equine osteocalcin (OC) quantification using purified equine OC as standard ... [more ▼]

This study describes for the first time the development and validation of a sensitive and specific radioimmunoassay (RIA) for equine osteocalcin (OC) quantification using purified equine OC as standard, tracer, and immunogen for antibody formation in rabbits. The assay allowed to measure equine serum OC levels with a sensitivity of 0.2 ng/mL. Immunoreactive serum OC values of clinically normal, different-aged horses ranged from 3.68 to 127.31 ng/mL. Intra- and inter-assay coefficients of variation (CV) were 6.2 and 8.2%, respectively. Serial equine scrum sample dilutions were linear. The recovery of equine OC from equine serum samples ranged from 93.88 to 107.9%. There was a tight correlation between OC values measured with the equine- specific OC RIA and two commercially available bovine-specific OC RIA kits. However, highest serum OC values were obtained with the equine-specific OC RIA. In conclusion, our equine-specific OC RIA is sensitive, linear, accurate, precise, and reproducible. The assay allowed to quantify OC in equine serum samples and might, therefore, be used to monitor equine osteoblast activity associated with bone diseases, exercise, therapy forms or diet. [less ▲]

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See detailDevelopment and validation of a UHPLC−LTQ−Orbitrap MS method for non-anthocyanin flavonoids quantification in Euterpe oleracea juice
Dias, A.L.; Rozet, Eric ULg; Larondelle, Y. et al

in Analytical and Bioanalytical Chemistry (2013)

Euterpe oleracea fruits have gained much attention because of their phenolic constituents that have shown potential beneficial effects for health. The aim of this work was to identify and quantify major ... [more ▼]

Euterpe oleracea fruits have gained much attention because of their phenolic constituents that have shown potential beneficial effects for health. The aim of this work was to identify and quantify major non-anthocyanin flavonoids in fruit juice by an accurate UHPLC−LTQ−Orbitrap MS method. Fruits were processed to juice, lyophilized and defatted. The residue was then extracted with methanol by sonication and the extraction time optimized giving recovery rates > 90%. Solubilization of dried extract was realized using 40% MeOH which showed the best compromise for MS detection. For the UHPLC quantification, a HSS C18 column (1.8µm) was used with a gradient elution of methanol and water both with 0.1% formic acid. Total error and accuracy profiles were used as validation criteria. Seven compounds and their isomers were successfully separated, including the major non-anthocyanin flavonoids. Calibration in the matrix was found to be more accurate than calibration without matrix. Trueness (< 15% relative bias), repeatability and intermediate precision (<13% RSD), selectivity, response function, linearity, LOD (ranged from 0.04 to 0.81 µg/mL) and LOQ (0.15 - 5.78 µg/mL) for 12 compounds were evaluated and the quantification method validated. Its applicability was demonstrated on real samples from different suppliers. Their qualitative and quantitative profiles were similar and some compounds were for the first time quantified. In addition eriodictyol was identified for the first time in this fruit along with 5 other flavonoids for which we proposed a possible structure. [less ▲]

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See detailDevelopment and validation of a ultra-high-performance liquid chromatography-UV method for the detection and quantification of erectile dysfunction drugs and some of their analogues found in counterfeit medicines
Sacré, Pierre-Yves ULg; Deconinck, Eric; Chiap, Patrice ULg et al

in Journal of Chromatography. A (2011), 1218

Pharmaceutical counterfeiting is a permanently growing problem. Control laboratories are constantly analysing counterfeit medicines. In industrialised countries, one of the main counterfeited class of ... [more ▼]

Pharmaceutical counterfeiting is a permanently growing problem. Control laboratories are constantly analysing counterfeit medicines. In industrialised countries, one of the main counterfeited class of medicines are erectile dysfunction drugs. This paper describes the development and validation of a fast method to detect and quantify the three authorised phosphodiesterase type 5 inhibitors and five analogues. The method is based on the use of a sub-2 microns polar-embedded column with a gradient using acetonitrile as organic modifier and 10 mM ammonium formate buffer (pH 3.5) as aqueous component of the mobile phase. The separation was achieved in less than 4.5 min. The method has also been compared to the registered HPLC method for the assay of Viagra® which was considered as the reference method. The method is also compatible with on-line coupling mass spectrometry and will significantly reduce analysis times and solvent consumption. [less ▲]

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See detailDevelopment and validation of an accelerometer-based method for quantifying gait events
Boutaayamou, Mohamed ULg; Schwartz, Cédric ULg; Stamatakis, Julien et al

in Medical Engineering & Physics (2015)

An original signal processing algorithm is presented to automatically extract, on a stride-by-stride basis, four consecutive fundamental events of walking, heel strike (HS), toe strike (TS), heel-off (HO ... [more ▼]

An original signal processing algorithm is presented to automatically extract, on a stride-by-stride basis, four consecutive fundamental events of walking, heel strike (HS), toe strike (TS), heel-off (HO), and toe-off (TO), from wireless accelerometers applied to the right and left foot. First, the signals recorded from heel and toe three-axis accelerometers are segmented providing heel and toe flat phases. Then, the four gait events are defined from these flat phases. The accelerometer-based event identification was validated in seven healthy volunteers and a total of 247 trials against reference data provided by a force plate, a kinematic 3D analysis system, and video camera. HS, TS, HO, and TO were detected with a temporal accuracy ± precision of 1.3 ms ± 7.2 ms, ‒4.2 ms ± 10.9 ms, ‒3.7 ms ± 14.5 ms, and ‒1.8 ms ± 11.8 ms, respectively, with the associated 95% confidence intervals ranging from ‒6.3 ms to 2.2 ms. It is concluded that the developed accelerometer-based method can accurately and precisely detect HS, TS, HO, and TO, and could thus be used for the ambulatory monitoring of gait features computed from these events when measured concurrently in both feet. [less ▲]

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See detailDevelopment and Validation of an Automated Method for the Liquid Chromatographic Determination of Sotalol in Plasma Using Dialysis and Trace Enrichment on a Cation-Exchange Pre-Column as on-Line Sample Preparation
Chiap, Patrice ULg; Ceccato, Attilio ULg; Miralles Buraglia, B. et al

in Journal of Pharmaceutical & Biomedical Analysis (2001), 24(5-6), 801-14

A fully automated method for the determination of sotalol in human plasma was developed, involving dialysis through a cellulose acetate membrane, clean-up and enrichment of the dialysate on a strong ... [more ▼]

A fully automated method for the determination of sotalol in human plasma was developed, involving dialysis through a cellulose acetate membrane, clean-up and enrichment of the dialysate on a strong cation-exchange pre-column and subsequent liquid chromatographic (LC) analysis with UV detection. All sample handling operations were carried out by means of an ASTED system. Before starting dialysis, the trace enrichment column (TEC) was conditioned. The plasma sample, to which the internal standard (atenolol) was automatically added, was then loaded in the donor channel and was kept static while the dialysis liquid, consisting of 0.017 M acetic acid, was passed through the acceptor channel in successive pulses. After each pulse, the dialysate was dispensed onto the TEC. When dialysis was discontinued, the analytes were eluted from the TEC by the LC mobile phase by rotation of a switching valve and transferred to the analytical column packed with octyl silica. The LC mobile phase was a mixture of methanol and pH 7.0 phosphate buffer containing 1-octanesulfonate at a concentration of 7.5 x 10(-4) M (19:81; v/v). The UV detection was performed at 230 nm. The influence of several parameters of the dialysis and trace enrichment processes on analyte recovery and method selectivity was investigated. The method was then validated. The mean absolute recovery for sotalol was about 60%. The limit of quantitation was 25 ng/ml and R.S.D. for repeatability and intermediate precision obtained at a concentration level of 50 ng/ml were 4.3 and 5.8%, respectively. [less ▲]

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See detailDevelopment and validation of analytical methods based on GC-MS/MC Triple Quadrupole instrument for the analysis of POPs in food and feed matrices
Calaprice, Chiara ULg

Doctoral thesis (2016)

This thesis focused on the development and validation of analytical methods for the de-tection and quantification of Persistent Organic Pollutants (POPs) in biological matrices, namely food and feed ... [more ▼]

This thesis focused on the development and validation of analytical methods for the de-tection and quantification of Persistent Organic Pollutants (POPs) in biological matrices, namely food and feed samples. POPs are a group of chemical compounds listed after the Stockholm Convention in 2001, with demonstrated toxicity and dangerousness for envi-ronment, animals and humans. In this work of thesis, special attention was reserved to some selected POPs: polychlo-rodibenzo-p-dioxins (PCDDs), polychlorodibenzofurans (PCDFs), usually referred to as “dioxins”, and polychlorinated biphenyls (PCBs), as there is a big concern about these contaminants in Taranto, a city in the Southern Italy very close to Bari, my home town. Taranto, indeed, is characterized by a large industrial area with a steel mill, several incin-erators and a refinery in few kilometre radius (Di Leo et al., 2014). This work has been done in collaboration with the University of Liège (Belgium) where a consolidated exper-tise in the field of POP measurements was available, especially in terms of dioxin anal-yses. In Chapter 1 the main steps, from sample preparation to data elaboration, of a validated confirmatory method for dioxin and PCB detection in food and feed using gas chromatog-raphy coupled to tandem mass spectrometry Triple Quad instrument (GC-MS/MS Triple Quad) have been described. This method was developed at the University of Liège in the framework of the last updates of the EU Regulation that in 2014 allowed confirmatory quantitative analysis of dioxins with Triple Quad. This method was the starting point of this work of thesis, because it was used for all dioxin and PCB quantifications. In Chapter 2 and chapter 3 alternative clean-up approaches for dioxin analysis in fatty food matrices have been developed using different automated systems. These works have been done in the framework of solvent and time saving for high throughput analytical methods in dioxin analysis. In chapter 2, an already existing automated system, DEXTechTM from LCTech GmbH (LCTech GmbH, Bahnweg 41, 84405 Dorfen, Germany) was used for sample clean-up, but a completely new clean-up approach was developed with this. GC-MS/MS Triple Quad instrument, as well as Magnetic Sector High Resolution Mass Spectrometry (HRMS) instrument were employed for final quantification, to demonstrate the suitability of our newly developed clean-up approaches whatever the instrumental detection. In Chapter 3 PowerPrepTM automated system from Fluid Management System (FMS Inc., 580 Pleasant Street, Watertown, MA 02472, USA) was used and our routine sample clean-up approach was modified in order to enhance the efficiency and to reduce cost and solvent consumption of the analysis. In Chapter 4 the main method for dioxin analysis was adapted for the integration of Dechloranes in the list of the analytes targeted in the regular control for dioxins in food and feed. Dechloranes are a family of 6 organo-chlorinated compounds with structure similar to Mirex, also called Dechlorane, a POP listed in the Stockholm convention. Dechloranes have been found in human blood of people from Europe (Brasseur et al., 2014) and in this work an analytical method for Dechlorane detection was developed and validated to investigate food as a possible route of exposure for humans in Europe, where no production plant has been identified so far. The analytical method was used to analyse 88 food and feed real sample and to give an idea of Dechlorane daily dietary intake. [less ▲]

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See detailDevelopment and Validation of LC Method for the Diclofenac Sodium Release Determination Using Accuracy Profile Concept
Al-Kamarany, M.A.; El Karbane, M; Belomaria, M et al

in Asian Journal of Chemistry (2012), 24(10), 4393-4400

The article presents a rapid and sensitive RP-HPLC method with UV detection, using the diclofenac sodium release determination in solid pharmaceutical formulations. A novel validation strategy based on ... [more ▼]

The article presents a rapid and sensitive RP-HPLC method with UV detection, using the diclofenac sodium release determination in solid pharmaceutical formulations. A novel validation strategy based on accuracy profiles was used to select the most appropriate regression model with highest accuracy within well defined acceptance limits. Furthermore, the strategy was used to determine the limits of quantification as well as the suitable concentration range. The validation phase was completed by investigating of the risk profiles of various acceptable regression models in order to avoid obtaining measurements outside the acceptance limits fixed a priori. On the other hand, the present study shows how the LC method can be used more accurately to assess the kinetic dissolution profiles, instead the UV-visible method required by monographs of the USP. Robustness study was also performed in order to demonstrate the capability of this method to remain unaffected by a small and deliberate variation in method parameters. The LC method was validated using the total error approach, as a decision tool, guarantees that each of the future results that will be within the acceptance limits settled at ± 5 %. The UV spectrophotometric method based on the USP monograph, gives rise of impurities from diclofenac sodium in acidic condition (HCl 0.1 N). These impurities absorb at the same wave length (276 nm) as the active principal ingredient, which will yield some significant error in the per cent release during the dissolution test study. Described analytical method is a simple, sensitive, specific and more accurate indicating that this LC method is useful for manufacturing and quality control assay. [less ▲]

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See detailThe development and validation of nursing related groups based on the Belgian Nursing Minimum Dataset
THONON, Olivier ULg; VAN HERCK, Pieter; GILLAIN, Daniel ULg et al

in SHEERIN, Fintan; SERMEUS, Walter; EHRENBERG, Anna (Eds.) ACENDIO 2013 - Proceedings of 9th European Conference of ACENDIO (2013, March)

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See detailDevelopment and validation of nursing resource weights for the Belgian Nursing Minimum Dataset in general hospitals: a Delphi questionnaire survey approach.
SERMEUS, Walter; GILLET, Pierre ULg; GILLAIN, Daniel ULg et al

in International Journal of Nursing Studies (2009), 46(2), 256-67

BACKGROUND: Internationally, nursing is not well represented in hospital financing systems. In Belgium a nursing weight system exists to adjust budget allocation for differences in nurse staffing ... [more ▼]

BACKGROUND: Internationally, nursing is not well represented in hospital financing systems. In Belgium a nursing weight system exists to adjust budget allocation for differences in nurse staffing requirements, but there is a need for revision. Arguments include the availability of a nursing minimum dataset and the adverse consequences of the current historically based nursing weight system. OBJECTIVES: The development and validation of nursing resource weights for the revised Belgium nursing minimum dataset (NMDS). DESIGN: Two independent cross sectional Delphi-surveys. SETTING AND PARTICIPANTS: A convenience sample of 222 head nurses from 69 Belgian hospitals participated in the cross sectional survey methods. To assess validity 112 patient case records from 61 nursing wards of 35 Belgian general hospitals representing general, surgical, pediatric, geriatric and intensive care were selected. METHODS: Nursing resource weights were constructed based on Delphi survey results by NMDSII intervention. The patient case Delphi survey results were used as the primary source for validation. A series of additional validation measures were calculated, based on the different patient classification systems. Finally, three validated nursing resource weighting systems were compared to the constructed NMDSII weighting system: the use of 'Closon', 'Ghent' and WIN weights. RESULTS: A coherent set of nursing resource weights was developed. The comparison of nurse resource weights, based on the survey per NMDS intervention versus the survey on patient cases, yielded high correlations: r=0.74 to r=0.97 (p<0.01) between three case rating questions, as an indication of reliability in terms of internal consistency, and r=0.90 (p<0.01) between summed intervention weights and patient case weights, as an indication of criterion validity in terms of concurrent validity. Other concurrent validity measures based on summed intervention weights versus patient classification dependency weights showed a correlation ranging from r=0.14 to r=0.74. The correlation of summed intervention weights with the Closon, Ghent and WIN weights ranged from r=0.93 to r=0.96 (p<0.01), as a third indication of concurrent validity. CONCLUSIONS: A system of valid nursing resource weights has been developed. The system should be further validated within an international context. [less ▲]

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See detailDevelopment and validation of situational judgment tests for evaluating Organizational Citizenship Behavior and Contextual Performance Behavior in a professional context : methodological issues.
Lothe, Benoit ULg; Hansez, Isabelle ULg

Conference (2011, June 22)

Strategic Human Resource Management typically raises appraisal issues (Analoui, 2007; Jackson, Schuler & Werner, 2009 ; Teo & Crawford, 2005) mostly related to the HRM relationship with organizational ... [more ▼]

Strategic Human Resource Management typically raises appraisal issues (Analoui, 2007; Jackson, Schuler & Werner, 2009 ; Teo & Crawford, 2005) mostly related to the HRM relationship with organizational performance (Bowen & Ostroff, 2004 ; Cascio, 1996 ; Wright, Gardner, Moynihan & Allen, 2005). Hence workforce assessment remains a key success factor for many organizations. Thus this paper takes place in the early beginning of a PhD Thesis which aims to the development and validation of an assessment test of Organizational Citizenship Behavior (OCB) and Contextual Performance (CP) in a professional selection context. The managerial concept of OCB refers to “individual behavior that is discretionary, not directly or explicitly recognized by the formal reward system, and in the aggregate promotes the efficient and effective functioning of the organization” (Organ, Podsakoff & MacKenzie, 2006, p. 3). CP is a closely related construct, distinct of task performance, that encompasses behaviors supporting the psychological and social context in which task activities are performed (Stone-Romero, Alvarez, & Thompson, 2009). If these two frameworks look much alike, they contrast on several points: conceptual dimensions, discretional characteristics of behavior, etc. Therefore, it seems interesting to apprehend both commonly. Situational Judgment Tests (SJTs) show a good validity concerning prediction of job performance in employment settings (Lievens, Peeters, Schollaert, 2008 ; Whetzel & McDaniel, 2009). Furthermore, they are a multidimentional measurement method suited to assess different construct like job knowlege and skills, applied social skills, and some basic personality tendencies (Christian, Edwards, & Bradley, 2010). Taking into account its characteristics, SJTs seem to be an appropriate method to enable OCB & CP evaluation. This communication pursues three goals. The first is a theoretical issue: we need a straightforward vision of OCB & CP concepts. Accordingly, after a consistent review on the vast amount of work that have been written on these two concepts, we have to specify what we seek to appraise and make the inventory of existing measurement tools related to these focus. We have already worked out a structured methodology to develop and validate SJTs in selection and career management context (Lothe, Hansez & Bertrand, Submitted). So the second issue is a methodological one. How to improve and/or to adapt this methodology in order to create a new SJT measuring OCB & CP ? Thirdly, some research related issues conclude this paper. Through this PhD project, we should be able to examine several main issues that will shape the thesis. Are SJTs being able to provide a valid assessment of OCB & CP in a personnel selection context ? Does the discretionary nature of one behavior impact the quality of its measurement ? What is the effect of response instructions on OCB/CP situational judgment test performance ? Each issue will be illustrated and discussed in connection with the development and validation opportunities and constraints. [less ▲]

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See detailDevelopment and validation of stable reference materials for food microbiology using Bacillus cereus and Clostridium perfringens spores
Abdelmassih, Marleen; Planchon, Viviane; Anceau, Christine ULg et al

in Journal of Applied Microbiology (2011)

To develop a new type of microbiological Reference Materials (RMs), displaying long-term stability at room temperature. The purpose was to produce and validate two batches of RMs for the enumeration of ... [more ▼]

To develop a new type of microbiological Reference Materials (RMs), displaying long-term stability at room temperature. The purpose was to produce and validate two batches of RMs for the enumeration of Bacillus cereus and Clostridium perfringens. Methods and Results: The RMs were based on spores of B. cereus and Cl. perfringens, adsorbed on calcium carbonate pellets. Two batches of 1000 units were manufactured and validated in compliance with ISO guide 35. After verification of their homogeneity, the stability of the ‘RM-B. cereus’ and ‘RMCl. perfringens’ batches was proven during at least 36 and 9 months, respectively, at room temperature. The validation study was completed by international collaborative trial involving 12 laboratories, allowing the validation of the assigned values. Conclusions: The methodology developed in this work enabled to produce easy-to-handle and cost-effective RMs, displaying an unprecedented stability at room temperature, a good homogeneity and a precise and validated assigned value. Significance and Impact of the Study: This study revealed new paths for the development of stable microbiological RMs. Overcoming the intrinsic instability of the living cells makes it possible to produce valuable tools for the quality assurance of microbiology laboratories. Jou [less ▲]

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See detailDevelopment and validation of the French version of a tool assessing patient's expectations in lower limb osteoarthritis
NEUPREZ, Audrey ULg; Delcour, Jean-Pierre; Fatemi, Firouzeh et al

in Journal of Orthopaedics (2015), 12

Objective: The Hospital for Special Surgery (HSS) Hip Replacement Expectations Survey and <br />Knee Replacement Expectations Survey are validated tools developed to measure patients' <br />preoperative ... [more ▼]

Objective: The Hospital for Special Surgery (HSS) Hip Replacement Expectations Survey and <br />Knee Replacement Expectations Survey are validated tools developed to measure patients' <br />preoperative expectations for hip and knee arthroplasty. These instruments have possible <br />uses in both daily practice and research. Our objective was to assess the test-retest reliability <br />and the construct validity of the French version of the surveys. <br />Methods: Patients scheduled for total hip (n ¼ 82) or knee replacement (n ¼ 61) aged 38e90 <br />years were included. All completed the HSS Hip or Knee Replacement Expectations Survey <br />and the Expectation WOMAC to determine concurrent validity. <br />The test-retest reliability was assessed using the intraclass coefficient correlation (ICC), <br />the Bland and Altman Method and the coefficient of variation; the internal consistency was <br />assessed by the Cronbach a coefficient. The construct validity was investigated using the <br />Pearson correlation coefficient and floor and ceiling effects by percentage frequency of <br />lowest or highest possible score achieved by respondents. <br />Results: 143 patients scheduled for hip or knee arthroplasty were included. <br />The reliability was excellent between the test and the rested total score, with an ICC of <br />0.902 (0.853e0.936) and CV of 4.06% for the French Hip Replacement Expectations Survey <br />and 0.865 (0.786e0.917) and CV of 7.7% for the French Knee Replacement Expectations <br />Survey, without bias. <br />The Cronbach a coefficient was 0.72 for hip Survey and 0.82 for knee Survey showing a <br />good internal consistency. <br />Pearson correlation coefficients of 0.45 and 0.48 between Expectations WOMAC and <br />HSS, respectively for hip Survey and knee Survey, were observed but with systematic bias. <br />The lowest possible score was not reported by any patient and only three patients <br />(3.66%) scheduled for hip arthroplasty reported the highest possible score. Conclusions: The French version of the HSS Hip or Knee Replacement Expectations Survey is <br />a reliable and valid questionnaire and compares favourably with the original English <br />version. Therefore, this new version may help French-speaking clinicians to evaluate expectations <br />before lower limb arthroplasty. [less ▲]

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