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See detailDetection of fruit trees viroids and epidemiological characteristics of the disease caused by Peach Latent Mosaic Viroid in Tunisia
Fekih Hassen, I.; Roussel, S.; Kummert, J. et al

Conference (2004)

Detailed reference viewed: 16 (1 ULg)
See detailDetection of fruit trees viroids and epidemiological characteristics of the disease caused by peach latent mosaic viroid in tunisia
Fekih Hassen, Imene; Roussel, Sophie; Kummert, Jean et al

Conference (2005)

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See detailDetection of glucocorticoid bioactivity in bovine urine samples using a reporter gene assay
Connolly, Lisa; Cai, Kai; Van Der Heiden, Edwige ULg et al

in Analytica Chimica Acta (2009), 637

The illegal use of anabolic substances in the meat producing industry is an ongoing problem due to the continual production of new synthetic compounds and/or the practice of lowlevel cocktail ... [more ▼]

The illegal use of anabolic substances in the meat producing industry is an ongoing problem due to the continual production of new synthetic compounds and/or the practice of lowlevel cocktail administration to avoid detection by the surveillance schemes of EU member states National Plan surveillance systems. We present a highly sensitive reporter gene assay and sample extraction procedure based on a two step solid phase extraction and high performance liquid chromatography, developed for the detection of glucocorticoid abuse in bovine urine. The assay is capable of detecting compounds with glucocorticoid bioactivity and is extremely sensitive with an EC50 of 0.79 ngmL−1 for dexamethasone. New or unknown compounds with glucocorticoid bioactivity and low-level cocktail mixtures are detectable by this assay. Cross-reactivity data for a range of 11 -hydroxyglucocorticoids has been provided. This assay shows low interference from the 11-keto prohormones and other steroidal hormones. The assay may be suitable for application in other matrices such as hair. In conclusion this screening assay offers advantages over existing analytical techniques. [less ▲]

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See detailDetection of glucocorticoid bioactivity in bovine urine samples using a reporter gene assay
Connolly, Lisa; Kai, C.; Van Der Heiden, Edwige ULg et al

in Proceedings of the Euroresidue VI Conference (2008)

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See detailDetection of Ground Movements on the Mt. Etna Volcano using the Global Positioning System
Bruyninx, Carine; Warnant, René ULg

in Acta Vulcanologica (1995), 7(1), 27-33

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See detailDetection of Heavy Metal Elements in Fume by Laser-Induced Breakdown Spectroscopy
Lin, Zhaoxiang; Li, Jie ULg; Liu, Linmei et al

in Chinese Journal of Lasers (2008), 35(12), 338-340

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See detailDetection of High molecular mass for MALDI Imaging and Profiling
Franck, Julien; Longuespée, Rémi ULg; Bonnel, David et al

Conference (2009)

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See detailDetection of High molecular mass for MALDI Imaging and Profiling
Franck, Julien; Longuespée, Rémi ULg

Conference (2009, September)

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See detailDetection of Hoxa1 Expression in Human Breast Cancer
Chariot, Alain ULg; Castronovo, Vincenzo ULg

in Biochemical and Biophysical Research Communications (1996), 222(2), 292-7

Homeodomain-containing proteins are transcription factors that regulate the coordinated expression of multiple genes involved in development, differentiation and malignant transformation. To better ... [more ▼]

Homeodomain-containing proteins are transcription factors that regulate the coordinated expression of multiple genes involved in development, differentiation and malignant transformation. To better understand the role played by these proteins in breast cancer cells, we demonstrate, using semi-quantitative RT-PCR experiments, that progestin induces HOXA1 mRNAs in MCF7 cells. This is the first evidence of regulation of a HOX gene by steroids. Moreover, we detected HOXA1 expression in a variety of human breast cancer lesions, suggesting that HOXA1 may be required for the establishment of breast cancer cells phenotype. We propose that HOXA1 gene could be one of the orchestrators that regulate breast epithelial cell differentiation and that alteration of HOXA1 expression could play a role in breast cancer progression. [less ▲]

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See detailDetection of HPV-induced cervical (pre)neoplastic lesions: a tissue microarray (TMA) study
Arafa, Mohammad Mahmoud Mohammad ULg; Boniver, Jacques ULg; Delvenne, Philippe ULg

in Applied Immunohistochemistry & Molecular Morphology (2008), 16(5), 422-432

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See detailDetection of Human Papillomaviruses in Paraffin-Embedded Biopsies of Cervical Intraepithelial Lesions: Analysis by Immunohistochemistry, in Situ Hybridization, and the Polymerase Chain Reaction
Delvenne, Philippe ULg; Fontaine, Marie-Anne; Delvenne, Catherine ULg et al

in Modern Pathology : An Official Journal of the United States & Canadian Academy of Pathology, Inc (1994), 7(1), 113-119

One hundred and forty biopsies with an initial diagnosis of cervical intraepithelial lesion (CIL) were tested for the presence of human papillomavirus (HPV) by immunohistochemistry and in situ ... [more ▼]

One hundred and forty biopsies with an initial diagnosis of cervical intraepithelial lesion (CIL) were tested for the presence of human papillomavirus (HPV) by immunohistochemistry and in situ hybridization using commercial biotinylated probes (Vira-Type in situ assay; Digene Diagnostics, Silver Spring, MD) or probes labeled with digoxigenin by the random primer technique. Immunohistochemistry was more inferior to the in situ hybridization method, with a detection rate of 14% (20/140) compared to 61% (86/140) for the in situ assay with the digoxigenin-labeled probes. Biotinylated probes proved to be slightly less sensitive than digoxigenin-labeled probes, with a detection rate of 53% (74/140). Although less sensitive in our series taken as a whole, immunohistochemistry was positive in a few cases of CILs negative by in situ hybridization, so that the association of these techniques gave the highest detection rate (66%; 92/140). The CILs that remained negative with these methods (34%; 48/140) were investigated by the polymerase chain reaction (PCR) using consensus primers to determine definitively the presence of HPV in these lesions and were reviewed histologically to assess the diagnosis of CILs. The PCR method increased the detection rate of HPV in our series to 76% (107/140). The diagnosis of CILs was confirmed for all the biopsy specimens positive by PCR (15/15; 100%) and for all the HPV negative tissues with histological features of a high-grade lesions (7/7; 100%).(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

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See detailDetection of hypoglycin A in the seeds of sycamore (Acer pseudoplatanus) and box elder (A. negundo) in New Zealand; the toxin associated with cases of equine atypical myopathy.
McKenzie, R. K.; Hill, F. I.; Habyarimana, Jean ULg et al

in New Zealand veterinary journal (2015)

CASE HISTORY AND CLINICAL FINDINGS: During April and May 2014 four horses aged between 5 months and 9 years, located in the Canterbury, Marlborough and Southland regions, presented with a variety of ... [more ▼]

CASE HISTORY AND CLINICAL FINDINGS: During April and May 2014 four horses aged between 5 months and 9 years, located in the Canterbury, Marlborough and Southland regions, presented with a variety of clinical signs including recumbency, stiffness, lethargy, dehydration, depression, and myoglobinuria suggestive of acute muscle damage. Two horses were subjected to euthanasia and two recovered. In all cases seeds of sycamore maple (Acer pseudoplatanus) or box elder (A. negundo) were present in the area where the horse had been grazing. LABORATORY INVESTIGATION: The samaras (seeds) of some Acer spp. may contain hypoglycin A, that has been associated with cases of atypical myopathy in Europe and North America. To determine if hypoglycin A is present in the samaras of Acer spp. in New Zealand, samples were collected from trees throughout the country that were associated with historical and/or current cases of atypical myopathy, and analysed for hypoglycin A. Serum samples from the four cases and four unaffected horses were analysed for the presence of hypoglycin A, profiles of acylcarnitines (the definitive diagnosis for atypical myopathy) and activities of creatine kinase and aspartate aminotransferase. Markedly elevated serum activities of creatine kinase and aspartate aminotransferase, and increased concentrations of selected acylcarnitines were found in the case horses. Hypoglycin A was detected in the serum of those horses but not in the healthy controls. Hypoglycin A was detected in 10/15 samples of samaras from sycamore maple and box elder from throughout New Zealand. DIAGNOSIS: Cases of atypical myopathy were diagnosed on properties where samaras containing hypoglycin A were also found. CLINICAL RELEVANCE: Sycamore and box elder trees in New Zealand are a source of hypoglycin A associated with the development of atypical myopathy. If pastured horses present with clinical and biochemical signs of severe muscle damage then the environment should be checked for the presence of these trees. Horses should be prevented from grazing samaras from Acer spp. in the autumn. [less ▲]

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See detailDetection of illegal growth promoters in biological samples using receptor binding assays
Scippo, Marie-Louise ULg; Van de Weerdt, Cécile ULg; Willemsen, Philippe et al

in Analytica Chimica Acta (2002), 473(1-2), 135-141

In the European Union (EU), the use of growth-promoting substances in meat production is banned. The control of growth promoters, especially steroid hormones, is presently based on expensive and time ... [more ▼]

In the European Union (EU), the use of growth-promoting substances in meat production is banned. The control of growth promoters, especially steroid hormones, is presently based on expensive and time-consuming chromatographic methods of analysis or, sometimes, for screening purposes, on radio- or enzyme-immunoassays, all of which are often too specific to allow effective multi-analyte control. In order to develop rapid and inexpensive multi-analyte detection tests, we proposed the use of hormonal receptors as detection tools. The system described here (radio-receptor assays) is based on a direct bindin g assay of steroid hormones to their respective receptors. Human receptors to estrogens (hERalpha), androgens (hAR), progestagens (hPR) and glucocorticoids (hGR) have been produced by genetic engineering in bacteria or in eucaryotic cells. Binding analyses revealed that the obtained receptor proteins retained a high affinity for their corresponding native ligand. In addition, competition studies continued that each of the four receptors displays a specificity profile for a series of analogs in agreement with the literature. Finally, the stability of these recombinant receptors is sufficient to allow their use in test kits. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailDetection of incoherent joint state due to inaccurate bone motion estimation
Schwartz, Cédric ULg; Leboeuf, Fabien; Rémy-Néris, Olivier et al

in Computer Methods in Biomechanics & Biomedical Engineering (2013), 16

In biomechanical modeling and motion analysis, the use of personalized data such as bone geometry would provide more accurate and reliable results. However, there is still a limited number of tools used ... [more ▼]

In biomechanical modeling and motion analysis, the use of personalized data such as bone geometry would provide more accurate and reliable results. However, there is still a limited number of tools used to measure the evolution of articular interactions. This paper proposes a coherence index to describe the articular status of contact surfaces during motion. The index relies on a robust estimation of the evolution of surfacic interactions between the joint surfaces. The index is first compared to distance maps on simulated motions. It is then used to compare two motion capture protocols (two different localizations of the markers for scapula tracking). The results show that the index detects progressive modifications in the joint and allows to distinguish the two protocols, in accordance with the literature. In the future, the index could, among other things, be used to compare / improve biomechanical models and motion analysis protocols. [less ▲]

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See detailDetection of influential observations on the error rate based on the generalized k-means clustering procedure
Ruwet, Christel ULg; Haesbroeck, Gentiane ULg

Conference (2009, October 14)

Cluster analysis may be performed when one wishes to group similar objects into a given number of clusters. Several algorithms are available in order to construct these clusters. In this talk, focus will ... [more ▼]

Cluster analysis may be performed when one wishes to group similar objects into a given number of clusters. Several algorithms are available in order to construct these clusters. In this talk, focus will be on the generalized k-means algorithm, while the data of interest are assumed to come from an underlying population consisting of a mixture of two groups. Among the outputs of this clustering technique, a classi cation rule is provided in order to classify the objects into one of the clusters. When classi cation is the main objective of the statistical analysis, performance is often measured by means of an error rate ER(F; Fm) where F is the distribution of the training sample used to set up the classi cation rule and Fm (model distribution) is the distribution under which the quality of the rule is assessed (via a test sample). Under contamination, one has to replace the distribution F of the training sample by a contaminated one, F(eps) say (where eps corresponds to the fraction of contamination). In that case, the error rate will be corrupted since it relies on a contaminated rule, while the test sample may still be considered as being distributed according to the model distribution. To measure the robustness of classification based on this clustering proce- dure, influence functions of the error rate may be computed. The idea has already been exploited by Croux et al. (2008) and Croux et al. (2008) in the context of linear and logistic discrimination. In this setup, the contaminated distribution takes the form F(eps)= (1-eps)*Fm + eps*Dx, where Dx is the Dirac distribution putting all its mass at x: After studying the influence function of the error rate of the generalized k- means procedure, which depends on the influence functions of the generalized k-means centers derived by Garcia-Escudero and Gordaliza (1999), a diagnostic tool based on its value will be presented. The aim is to detect observations in the training sample which can be influential for the error rate. [less ▲]

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See detailDetection of interstellar CH and CH(+) towards SN 1987a
Magain, Pierre ULg; Gillet, D.

in Astronomy and Astrophysics (1987), 184

The authors report the detection of interstellar CH and CH[SUP]+[/SUP] towards supernova 1987A. For both of these molecules, one component is detected at a heliocentric velocity of about 280 km/s ... [more ▼]

The authors report the detection of interstellar CH and CH[SUP]+[/SUP] towards supernova 1987A. For both of these molecules, one component is detected at a heliocentric velocity of about 280 km/s, corresponding to material inside the LMC. Some implications of the authors' results with regards to the H[SUB]2[/SUB]-to-dust ratio are briefly discussed. [less ▲]

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See detailDetection of irregularities in the TEC using GPS measurements. Application to a mid-latitude station
Warnant, René ULg

in Acta Geodaetica et Geophysica Hungarica (1998), 33(1), 121-128

Detailed reference viewed: 20 (2 ULg)
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See detailDetection of laminin receptor mRNA in human cancer cell lines and colorectal tissues by in situ hybridization.
Campo, E.; Monteagudo, C.; Castronovo, Vincenzo ULg et al

in American Journal of Pathology (1992), 141(5), 1073-83

The 67-kd high-affinity laminin receptor (67 LR) is a gene product whose expression appears to be associated with the invasive and metastatic phenotype of a variety of human cancer cells. Northern blot ... [more ▼]

The 67-kd high-affinity laminin receptor (67 LR) is a gene product whose expression appears to be associated with the invasive and metastatic phenotype of a variety of human cancer cells. Northern blot hybridization has been routinely used to quantify the level of 67 LR mRNA from total cellular RNA extracts of homogenized tissue specimens or in vitro grown cell populations. This technique is useful to assess the average expression of the 67 LR mRNA of a particular sample but does not provide information about expression in specific cell types nor about heterogeneity of expression from cell to cell. In this study, we analyzed the expression of 67 LR mRNA in four human cancer cell lines with varying degrees of expression of 67 LR protein (renal cancer A-704, breast carcinoma MCF-7/4 and MCF-7/7, and pancreatic cancer Panc-1) using in situ hybridization performed with 67 LR riboprobes. Total cellular RNA was simultaneously extracted from the cell lines and hybridized on Northern blots with a 67 LR cDNA probe to assess the validity of the mRNA detection by in situ hybridization. Sixty-seven LR mRNA expression was higher in Panc-1 and MCF-7/4 cells than in MCF-7/7 and renal carcinoma A-704. There was a direct correlation (R2 = 0.88) between the in situ hybridization analysis and the mRNA levels detected by Northern blot analysis. The in situ hybridization method showed a heterogeneous expression of the 67 LR mRNA in the four cell lines with different subpopulations of cells showing a range from negative to high levels of the message. Sixteen freshly frozen human colorectal tissues (seven adenocarcinomas, five matched normal mucosae, and four adenomas) were also analyzed by in situ hybridization. The 67 LR mRNA was localized in normal and neoplastic epithelial cells. Adenocarcinoma cells showed a 1.6- to 5-fold higher expression (P < 0.02 according to the Wilcoxon-Mann-Whitney test) than did epithelial colonic cells from normal mucosae or adenomas. The signal tended to be stronger in poorly differentiated carcinomas and carcinomas with metastases than in moderately differentiated and nonmetastatic tumors. We conclude that the high expression of 67 LR mRNA in colorectal tumors is due to an increased production by tumor cells. Furthermore, in situ hybridization is an effective method to detect the expression of LR mRNA in cultured cell lines as well as in frozen tissue sections. [less ▲]

Detailed reference viewed: 14 (0 ULg)