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Peer Reviewed
See detailDetermination of Non-Steroidal Anti-Inflammatory Drugs by Capillary Zone Electrophoresis
Bechet, I.; Paques, Ph.; Fillet, Marianne ULg et al

Poster (1994)

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Peer Reviewed
See detailDetermination of optimal load shedding against voltage instability
Moors, Cédric; Van Cutsem, Thierry ULg

in Proc. 13th Power System Computation Conference (PSCC) (1999, June)

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See detailDetermination of optimum conditions for preservation and reactivation of freeze-dried Acetobacter senegalensis used as vinegar starter
Shafiei, Rasoul ULg; Thonart, Philippe ULg

Poster (2011, November)

Availability of efficient starters is one of the most important elements during fermentation technology. Acetic acid bacteria (AAB) face extreme conditions (low pH, high acetic acid concentration (>7 ... [more ▼]

Availability of efficient starters is one of the most important elements during fermentation technology. Acetic acid bacteria (AAB) face extreme conditions (low pH, high acetic acid concentration (>7%), ethanol and high temperature) during vinegar fermentation. In spite of introduction of broth media contained viable AAB as starter by some companies; traditional methods for initiation of vinegar fermentation are still used even in modern factories, therefore there is a long Lag phase for initiation of fermentation. In recent studies in CWBI, a kind of lyophilized starter has been produced by using an isolated thermotolerant bacterium: Acetobacter senegalensis. The aim of this study is to determine the best media for revitalization and the optimum temperature for preservation of the freeze-dried cells. To produce biomass, glucose was used as carbon source in batch culture under regulated conditions. The cells harvested when they reached to stationary phase. Harvested cells suspended in spent growth medium (final supernatant of fermentation). Maltodextrin (10%), manitol (20%) and spent growth medium were used as protestants. After freeze-drying they were subjected to storage test at -20° C, 4° C and 35° C for six months. The viability of cells determined by using spread plate technique using three different media: YG (yeast extract, glucose) and YGE (yeast extract, glucose, ethanol) and YGEA (yeast extract, glucose, ethanol, acetic acid). According to residual viable cells, manitol and maltodextrin showed higher protective functions rather than spent growth medium (92.3%, 88.2% and 82.1% survival, respectively) during freeze-drying process. Viability of cells during rehydration is completely dependent to the composition of the broth media used for this purpose. Adding ethanol (>0.5%) or acetic acid (>1%) to rehydration medium caused 40-45% reduction in viable cell numbers in comparison to YG broth. The composition of culture media can also influence the growth of bacteria after rehydration. In YG, much higher cell growth (about 1 log unit) was observed in comparison to YGE and YGEA. Addition of glucose (20 g/l) to YGE can neutralize the adverse effect of ethanol considerably but it cannot improve the growth in YGEA. This can be partially explained according to the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase. It was revealed that freeze-dried cells had very low activities for these two enzymes. Analysis of total soluble protein contents of lyophilized cells during storage at different conditions revealed that the soluble protein content of cells reduced by increasing the storage temperature. At 35° C after 15 days, there were 4.87 log units reduction in the viability of cells, and also about 14% less soluble protein was detectable under this condition whereas keeping of cells at -20 or + 4° C had no influences on protein and viability of cells. Storage of cells at +4° C in darkness for six month showed that about 79.2% and 68.3% viability of cells remained for cells protected by manitol and maltodextrin, respectively. To assess the real activity of lyophilized cells as starters, they were introduced to broth media (YGE contained 20g/l of glucose). It was seen that 6 months old lyophilized cells (with maltodextrin or manitol as protectants) were able to grow and consume 5 v/v of ethanol in 48 hr in the presence of initial acetic acid concentration (0.5%) at 30° C. In conclusion, the mentioned methods for preservation and reactivation of freeze-dried Acetobacter senegalensis can provide a promising tool for decreasing the lag phase of vinegar fermentation. [less ▲]

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See detailDetermination of organochlorine pesticide residues in the blood of healthy individuals
Charlier, Corinne ULg; Plomteux, Guy ULg

in Clinical Chemistry & Laboratory Medicine (2002), 40(4), 361-364

Pesticide use is one of several factors that have permitted maintenance of our supply of food in spite of continuing increase of the population. However, the use of biologically active compounds poses ... [more ▼]

Pesticide use is one of several factors that have permitted maintenance of our supply of food in spite of continuing increase of the population. However, the use of biologically active compounds poses potential problems of toxicity. If the compound is used at any stage of food production, residues or derivatives may persist in food and the entire population may be exposed to the trace amounts of the material. The human body burden associated with long-term exposure may or may not be associated with illness. Persistent environmental contaminants such as pesticide residues have long been suspected to be implicated in cancer etiology. Organochlorine chemicals are persistent, lipophilic compounds commonly present in the environment. Some of them demonstrated carcinogenic activity in laboratory animals. Controversy still exists concerning their carcinogenic potential in humans. To answer this question, clinical toxicology laboratories should propose validated methodologies able to identify and quantify pesticide residues in biological samples. An example of chromatographic method dedicated to organochlorine residues is presented here and illustrated by results obtained in a healthy population (104 men, 147 women). Only 17.9% of the samples were free from detectable amounts of pesticides and p,p’-DDE 1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene was the most frequently detected residue (66.5%). Hexachlorobenzene was found at detectable level in 13.5% of the samples. [less ▲]

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See detailDetermination of organohalogenated contaminants in liver of harbour porpoises (Phocoena phocoena) stranded on the Belgian North Sea coast
Covaci, Adrian; Van de Vijver, Kristin Inneke; De Coen, Wim et al

in Marine Pollution Bulletin (2002), 44(10),

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See detailDetermination of PAG in the milk and blood in cow’s in comparison to other examination methods for pregnancy diagnosis.
Gajewski, Z.; Melo de Sousa, Noelita ULg; Konopka, A. A. et al

in Magyar Allatorvosok Lapja = Hungarian Veterinary Journal (2008), (Suppl II), 190

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See detailDetermination of Particle Size, Surface Area, and Shape of Supplementary Cementitious Materials by Using Different Techniques
Arvaniti, Eleni; Juenger, Maria; Bernal, Susan et al

in Materials and Structures (2015), 48(11), 3687-3701

The particle size distribution, surface area and shape are fundamental characteristics of supplementary cementitious materials (SCMs). Accurate measurement of these properties is required in computational ... [more ▼]

The particle size distribution, surface area and shape are fundamental characteristics of supplementary cementitious materials (SCMs). Accurate measurement of these properties is required in computational efforts to model the hydration process, and the characterization of these parameters is also an important practical issue during the production and use of blended cements. Since there are no standard procedures specifically for the determination of physical properties of SCMs, the techniques that are currently used for characterizing Portland cement are applied to SCMs. Based on the fact that most of the techniques have been developed to measure cements, limitations occur when these methods are used for other materials than cement, particularly when these have lower fineness and different particle shape and mineralogical composition. Here, samples of fly ash, granulated blast furnace slag and silica fume were tested. Different results obtained using several methods for the determination of specific surface area are presented. Recommendations for testing SCMs using air permeability, sieving, laser diffraction, BET, image analysis and MIP are provided, which represent an output from the work of the RILEM Technical Committee on Hydration and Microstructure of Concrete with Supplementary Cementitious Materials (TC-238-SCM). [less ▲]

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See detailDetermination of phospholipids extracted from Tsukamurella paurometabola C-924 after freeze-drying and during the subsequent storage
Coulibaly, I.; Dauphin, R. D.; Destain, Jacqueline ULg et al

Scientific conference (2010)

The total lipids of Tsukamurella paurometabola C-924 were analyzed after freeze-drying. Seven individual lipids classes were identifiednamelyneutrallipids(NLs),fattyacids(FAs),phospholipids(PLs ... [more ▼]

The total lipids of Tsukamurella paurometabola C-924 were analyzed after freeze-drying. Seven individual lipids classes were identifiednamelyneutrallipids(NLs),fattyacids(FAs),phospholipids(PLs),sterolester(SEs),triglycerides(TGs),diglycerides (DGs) and monoglycerides (MGs). The principal fatty acids identified in most lipid classes were palmitic (C 16:0), palmitoleic (C 16:1), oleic (C 18:1), linoleic (C 18:2), and linolenic (C 18:3). PLs were the major constituents and accounted for 50-60% of the total lipids. PLs were fractionated. PLs of Tsukamurella paurometabola content phosphatidic acid (PA), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine (PC), sphingomyelin (SM), lysophosphatidylcholine (LPC) and phosphatidylglycerol (PG). It was observed that PG had the highest proportion at most points relative to other PLs and was the predominant component of PLs (30%-56%). Evolution of individual rate was followed during storage at 20°C and 40°C with or without lithothamne400®, respectively. [less ▲]

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See detailDetermination of phospholipids extracted from Tsukamurella paurometabola C-924 after freeze-drying and during the subsequent storage.
Coulibaly, Ibourahema ULg; Yao, Amenan Anastasie ULg; Dubois Dauphin, Robin ULg et al

Poster (2009, April 01)

The total lipids of Tsukamurella paurometabola C-924 were analysed after freeze-drying. Seven individual lipids classes were identified namly neutral lipids (NLs), fatty acids (FAs), phospholipids (PLs ... [more ▼]

The total lipids of Tsukamurella paurometabola C-924 were analysed after freeze-drying. Seven individual lipids classes were identified namly neutral lipids (NLs), fatty acids (FAs), phospholipids (PLs), sterol ester (SEs), triglycerides (TGs), diglycerides (DGs) and monoglycerides (MGs). The principal fatty acids identified in most lipid classes were palmitic (C16:0), palmitoleic (C16:1), oleic (C18:1), linoleic (C18:2), and linolenic (C18:3). PLs were the major constituents and accounted for 50-60% of the total lipids. PLs were fractionated. . PLs of Tsukamurella paurometabola content phosphatidic acid (PA), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine (PC), sphingomyelin (SM), lysophosphatidylcholine (LPC) and phosphatidylglycerol (PG). It was observed that PG had the highest proportion at most points relative to other PLs and was the predominant component of PLs (30%-56%). Evolution of individual rate was followed during stored at 20°C and 40°C with or without lithothamne400®, respectively. [less ▲]

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See detailDetermination of photodissociation and radiative association cross sections from the same time-dependent calculation
Vranckx, Stéphane; Loreau, Jérôme; Desouter, Michèle ULg et al

in Journal of Physics : B Atomic Molecular & Optical Physics (2013), 46

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See detailDetermination of physical changes of inulin related to sorption isotherms: An X-ray diffraction, modulated differential scanning calorimetry and environmental scanning electron microscopy study
Ronkart, Sébastien ULg; Blecker, Christophe ULg; Fougnies, C. et al

in Carbohydrate Polymers (2006), 63(2), 210-217

This paper gives a relationship between powdered inulin behaviour and physical parameters determination. Glass transition temperature (T,) and development of crystallinity were measured in relation to the ... [more ▼]

This paper gives a relationship between powdered inulin behaviour and physical parameters determination. Glass transition temperature (T,) and development of crystallinity were measured in relation to the water moisture of the polymer. These parameters were obtained by Modulated Differential Scanning Calorimetry (MDSC) and powder X-ray diffraction, respectively. In order to change the water content of the powder, adsorption and desorption isotherms (using different relative humidity storage conditions) were done and successfully fitted to the Guggenheim-Anderson-de Boer (GAB) model. Dependent on the relative humidity storage, a caking phenomenon occurred when glass transition temperature was under storage temperature. An Environmental Scanning Electron Microscopy (ESEM) study showed a structural change when water activity increased above 0.56 at 20 degrees C. A correlation between the increase of the crystallinity and the hardening of the powder was determined. ESEM permitted an observation of the development of some crystal structures among the amorphous system, confirmed by an increase of the diffraction peaks obtained by powder X-ray diffraction. These observations lead to an understanding of the physical characteristics of inulin related to the water moisture. (c) 2005 Elsevier Ltd. All rights reserved. [less ▲]

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See detailDetermination of plant height for weed detection in stereoscopic images
Piron, Alexis ULg; Leemans, Vincent ULg; Kleynen, Olivier et al

in AGENG 2008 Conference - Agricultural & Biosystems Engineering for a Sustainable World (2008)

The aim of this study was twofold. The first goal was to acquire high accuracy stereoscopic images of small-scale field scenes, the second to examine the potential of plant height as a discriminant factor ... [more ▼]

The aim of this study was twofold. The first goal was to acquire high accuracy stereoscopic images of small-scale field scenes, the second to examine the potential of plant height as a discriminant factor between crop and weed, within carrot rows. Emphasis was put on how to determine actual plant height taking into account the variable distance from camera to ground and ground irregularities for in-field measurements. Multispectral stereoscopic images were taken over a period of 19 days starting one week after crop emergence and seven weed species were considered. Images were acquired with a mobile vision system consisting in a filter wheel based multispectral camera and a video projector. The stereoscopy technique used belonged to the coded structured light family. The stereoscopic acquisition method yielded good results despite the numerous stereoscopic difficulties exhibited by the scenes. A plant height parameter as opposed to distance from camera to plant pixels gave better results for classification (classification accuracy of up [less ▲]

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See detailDetermination of pole orientations and shapes of asteroids.
Magnusson, P.; Barucci, M. A.; Drummond, J. D. et al

in Binzel; Gehrels, T.; Matthews, M. S. (Eds.) Asteroids II (1990)

The principles of asteroid lightcurve inversion and the information available from photometry are reviewed. General tools as well as specific techniques for shape and pole determinations are summarized ... [more ▼]

The principles of asteroid lightcurve inversion and the information available from photometry are reviewed. General tools as well as specific techniques for shape and pole determinations are summarized and their advantages and shortcomings are discussed. The authors also present the results obtained so far in this very active field and discuss their significance in the general context of asteroid research and planetary formation. [less ▲]

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See detailDetermination of polyisoprene-block-poly(methyl methacrylate) domain sizes using 1H spin diffusion
Werkhoven, Thekla M; Mulder, Fokko M; Zune, Catherine et al

in Macromolecular Chemistry and Physics (2003), 204(1), 46-51

The microphase structure of polyisoprene-block-poly(methyl methacrylate) diblock copolymers was studied using solid-state NMR techniques. Wideline separation spectroscopy reveals a narrow interphase ... [more ▼]

The microphase structure of polyisoprene-block-poly(methyl methacrylate) diblock copolymers was studied using solid-state NMR techniques. Wideline separation spectroscopy reveals a narrow interphase between the two polymers. The domain sizes of a lamellar sample and a sample with hexagonally ordered cylinders were determined using 1H spin diffusion. The lamellar sample shows a domain size of 16 ± 2 nm for the minor polyisoprene phase and a long period of 33 ± 4 nm. The cylindrical structure has a long period of 38 ± 7 nm, the diameter of the PMMA cylinders is 21 ± 4 nm. These results are about 20% below the estimates obtained from theoretical calculations according to Helfand and Wasserman [less ▲]

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See detailDetermination of porcine plasma follitropin levels during superovulation treatment in cows
Demoustier, M.; Beckers, Jean-François ULg; Van Der Zwalmen, P. et al

in Theriogenology (1988), 30(2), 379-386

Porcine follicle stimulating hormone (pFSH) and porcine luteinizing hormone (pLH), are widely used to induce superovulation in cows. An advantage of this treatment is that the LH:FSH ratio can be varied ... [more ▼]

Porcine follicle stimulating hormone (pFSH) and porcine luteinizing hormone (pLH), are widely used to induce superovulation in cows. An advantage of this treatment is that the LH:FSH ratio can be varied to optimize the growth of the ovarian follicles. However, due to the relatively short half-life of FSH, the superovulatory treatment requires numerous injections. A performant radioimmunoassay system (sensitivity=0.2 ng/ml plasma) was used to determine plasma pFSH levels in cows that were superovulated with 2 daily injections of 4 Armour Units (A.U.) of pFSH for 4 d. From plasma profiles, the half-life and the disappearance of pFSH were estimated at 5 h and at 10 to 12 h, respectively, confirming the necessity of using two daily injections. [less ▲]

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See detailDetermination of pregnancy-associated glycoprotein concentrations in goats (Capra hircus) with unsuccessful pregnancies: a retrospective study
Zarrouk, A.; Engeland, I.; Sulon, Joseph ULg et al

in Theriogenology (1999), 51(7), 1321-31

Presented here are the profiles of pregnancy-associated glycoprotein (PAG) concentrations in blood collected weekly from goats experiencing maintained and unsuccessful pregnancies. The analysis of these ... [more ▼]

Presented here are the profiles of pregnancy-associated glycoprotein (PAG) concentrations in blood collected weekly from goats experiencing maintained and unsuccessful pregnancies. The analysis of these profiles clearly indicated 4 different situations: the pseudopregnancy syndrome, abortion between Days 89 and 137, parturition of 1 dead and 1 live fetus, and expulsion of macerated or mummified fetuses after full term. A marked reduction in PAG concentration at any time during pregnancy was followed by an event such as abortion or the expulsion of a dead fetus at term or later. [less ▲]

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See detailDetermination of Pregnancy-Associated Glycoprotein concentrations in sheep: a retrospective study
Gajewski, Zdzislaw; Beckers, Jean-François ULg; Melo de Sousa, Noelita ULg et al

in Advances in Cell Biology (1999), 26(Supplement 12), 89-96

This study is carried out to determine ovine pregnancy associated glycoprotein (oPAG) levels in the serum of Berrichone ewes throughout gestation and the first month post partum. The levels were ... [more ▼]

This study is carried out to determine ovine pregnancy associated glycoprotein (oPAG) levels in the serum of Berrichone ewes throughout gestation and the first month post partum. The levels were determinated with heterologous RIA using bovine PAG as standard and tracer and rabbit antiserum against oPAG. The level of oPAG increased from the 4th week of pregnancy till 12-13th week and decreased slowly till 17th week, when increased rapidly and reached the highest level (around 600-700 ng/ml). In the sheep carrying twins, we observed higher levels of oPAG than in the uniparous sheep. After lambing, oPAG decreased rapidly in 4 week to basal values. The oPAG concentrations in the animals carrying twins was higher when comparised to single lamb. We concluded that the oPAG level depends on the number of lambs and term of pregnancy. The estimation of oPAG concentrations in the blood can be used as a indicator for pregnancy diagnosis. [less ▲]

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See detailDetermination of pregnancy-associated glycoprotein, P4, E1S and PgFM concentrations in the sheep during pregnancy
Gajewski, Z.; Beckers, Jean-François ULg; Thun, R.

in Proceedings: 23rd World Buiatrics Congress, Québec, Canada (2004)

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See detailDetermination of pregnancy-associated glycoproteins (bPAG) in cow’s milk
Metelo, R.; Silva, S.; Sulon, J. et al

in Proceedings: 23rd World Buiatrics Congress, Québec, Canada (2004)

Detailed reference viewed: 23 (0 ULg)