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See detailEffect of different kinds of anoxia/reoxygenation on the mitochondrial function and the free radicals production of cultured primary equine skeletal myoblasts.
Ceusters, Justine ULg; Mouithys-Mickalad, Ange ULg; Franck, Thierry ULg et al

in Research in Veterinary Science (2013), 95

Horses are outstanding athletes, performing in many different disciplines involving different kinds of efforts and metabolic responses. Depending on exercise intensity, their skeletal muscle oxygenation ... [more ▼]

Horses are outstanding athletes, performing in many different disciplines involving different kinds of efforts and metabolic responses. Depending on exercise intensity, their skeletal muscle oxygenation decreases, and the reperfusion at cessation of the exercise can cause excessive production of free radicals. This study on cultured primary equine myoblasts investigated the effect of different kinds of anoxia/reoxygenation (A/R) on routine respiration, mitochondrial complex I specific activity and free radicals production. Our data revealed that short cycles of A/R caused a decrease of all the parameters, opposite to what a single long period of anoxia did. A preconditioning-like effect could explain our first pattern of results whereas mild uncoupling could be more appropriate for the second one. Anyway, it seems that mitochondrial complex I could play a major role in the regulation of the balance between metabolic and antioxidant protection of the muscular function of athletic horses. [less ▲]

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See detailEffect of different protectants on viability of thermotolerant acetic acid bacterium Acetobacter sengalenisis
Shafiei, Rasoul ULg; Thonart, Philippe ULg

in Annual Confernace of the Association for general and applied microbiology (VAAM) (2011, April)

Production of vinegar at high temperature (>37° C) needs special processes and equipments; one of the key elements in the process, is the accessibility of active and stable starters. In this study the ... [more ▼]

Production of vinegar at high temperature (>37° C) needs special processes and equipments; one of the key elements in the process, is the accessibility of active and stable starters. In this study the influences of different cryo-protective agents on some steps (freezing, drying and storage) of starter production were investigated. To achieve this goal, Acetobacter senegalensis, was used as a thermotolerant acetic acid bacterium. Glucose was used as carbon source in fermentor to produce biomass. Different cryo-protectants (manitol (20%), glycerol (3%), sucrose (10%), trehalose (5%), glutamate (3%), maltodextrin (10%), skimmed milk (10%) and spent growth medium) were added to washed and unwashed biomass. The lyophilized cells (92-93% water content) were stored in darkness under different temperatures (-20° C, +4° C and 35° C). The viability of cells after rehydration, activity of glucose dehydrogenase, gluconate dehydrogenase and soluble protein contents were determined up to 6 months. According to the results, washing of cells by tap water has no effect on viability of cells during freezing and more than 97% of cells are alive in all treatments. After lyophilization, unwashed cells showed higher viability in all treatments in comparison to washed cells. On the basis of residual viable cells, manitol, maltodextrin, and spent growth medium showed the highest protective effects (92.3%, 88.2% and 82.1% survival, respectively) on cells during drying process whereas glycerol had the lowest protective effect on viability (15.4% survival). During storage of lyophilized cells at 35° C, 100% of cells are dead in all treatments after 15 days. Unwashed cells treated with manitol, maltodextrin and spent growth medium showed 79.2%, 68.3% and 62.7% viability, respectively after keeping at 4°C for 6 months. There is direct relationship between the soluble protein contents of cells and storage temperature. Cells stored at -20° C showed highest soluble protein contents after 6 months of storage while the lowest amount of soluble protein contents was detected in cells stored at 35° C. On the other hand, glucose dehydrogenase and gluconate dehydrogenase activities decreased during storage of cells at 4°C, whereas more than 90% of the enzymes activity remained during storage of different cells at -20° C, so it can be assumed that higher temperature can inactivate cell proteins. In conclusion, lyophilization of Acetobacter senegalensis by the mentioned methods can provide a promising and economic tool for production of stable and active vinegar starters. [less ▲]

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See detailEffect of different treatment protocols on ovulation and pregnancy rate in dairy cattle.
Repasy, A.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

in Magyar Allatorvosok Lapja = Hungarian Veterinary Journal (2008), (Suppl II), 314

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See detailEFFECT OF DIFFERENT WARMING-UP PROTOCOLS ON TIME TO EXHAUSTION AT MAXIMAL AEROBIC SPEED
Rodriguez de la Cruz, Carlos ULg; Brennenraedts, C.; Bury, Thierry ULg

in British Journal of Sports Medicine (2011), 45

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See detailEffect of digital nerve stimuli on responses to electrical or magnetic stimulation of the human brain.
MAERTENS DE NOORDHOUT, Alain ULg; Rothwell, J. C.; Day, B. L. et al

in Journal of Physiology (1992), 447

1. Reflexes were elicited in the first dorsal interosseous muscle of seven normal subjects by electrical stimulation of the digital nerves of the index finger at 3 times perceptual threshold while ... [more ▼]

1. Reflexes were elicited in the first dorsal interosseous muscle of seven normal subjects by electrical stimulation of the digital nerves of the index finger at 3 times perceptual threshold while subjects maintained a constant voluntary contraction of the muscle. The average response in the surface-rectified electromyogram (EMG) consisted of an early inhibitory (I1) component followed by a later excitation (E2). 2. Low intensity anodal electrical or magnetic scalp stimuli were given over the motor cortex in order to elicit muscle responses within the period of the I1 and E2 reflex components. 3. Compared with control responses elicited in the absence of digital nerve stimulation, responses to electrical cortex stimulation were suppressed in the I1 period and facilitated during the E2 period of the reflex. In contrast, responses evoked by magnetic stimulation were suppressed during I1 and also for the first 10 ms or so of the E2 response. Magnetically evoked responses were facilitated during the later part of the E2 reflex. 4. Similar effects were seen when the probability of firing of single motor units was studied. 5. In three subjects, small taps were given to the abducted index finger in order to stretch the first dorsal interosseous muscle and evoke reflexes which were of comparable size to the E2 reflex evoked by digital nerve stimulation. In contrast to the experiments in which digital nerve stimuli were given, responses evoked by magnetic stimulation over motor cortex were facilitated at all times during the course of the reflex evoked when the muscle was stretched. 6. We conclude that single electrical stimuli applied to the digital nerves can reduce for a short period the excitability of motor cortex to magnetic stimulation. This occurs at a time when the same stimulus is evoking an excitatory (E2) reflex in the average surface-rectified EMG. [less ▲]

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See detailThe effect of dispersion and atomisation conditions on the compaction response of silicon nitride granules
Destoop, Vincent; Mertens, Anne ULg; Hendrix, W. et al

in Key Engineering Materials [=KEM] (1997), 132-136

The relationship between the dispersion conditions of silicon nitride slurries and the compaction behaviour of spray dried granules is reported in the case of two commercial powders. Two parameters have ... [more ▼]

The relationship between the dispersion conditions of silicon nitride slurries and the compaction behaviour of spray dried granules is reported in the case of two commercial powders. Two parameters have been retained: the solid loading, varied between 10 and 30 vol.% and the degree of stability of the suspension, controlled by adjustment of the pH. [less ▲]

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See detailEffect of DNA photosensitization mediated by promazine derivatives on transcription in vitro
Decuyper, J.; Piette, Jacques ULg; Rentier, Bernard ULg et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1984), 92(2), 18

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See detailEffect of dopamine and baclofen on N-methyl-D-aspartate-induced burst firing in rat ventral tegmental neurons.
Seutin, Vincent ULg; Johnson, S. W.; North, R. A.

in Neuroscience (1994), 58(1), 201-6

Intracellular microelectrode recordings were made from dopamine-containing neurons of the ventral tegmental area or substantia nigra zona compacta in rat brain slices in vitro. The firing pattern of the ... [more ▼]

Intracellular microelectrode recordings were made from dopamine-containing neurons of the ventral tegmental area or substantia nigra zona compacta in rat brain slices in vitro. The firing pattern of the neurons was switched from a tonic, single-spike pattern to a burst firing mode by adding N-methyl-D-aspartate (20 microM) to the superfusing solution; after adding tetrodotoxin the membrane potential underwent rhythmical oscillations of 20-40 mV at 0.5-2 Hz. Baclofen (1 microM) and dopamine (30 microM) hyperpolarized the neurons; when the potential was restored to its original level, the oscillations of potential and/or burst firing were not observed, but the tonic firing pattern was restored. These effects of baclofen and dopamine were prevented by barium (1 mM), which also prevented the membrane hyperpolarization. Oscillations of membrane current of a similar frequency were observed when the somatic membrane was voltage-clamped at -60 mV; these were also blocked by barium (1 mM). It is concluded that the oscillations in membrane potential observed with N-methyl-D-aspartate are generated predominantly at a dendritic location which is not voltage-clamped with an electrode at the soma. Baclofen and dopamine inhibit the oscillations by increasing the potassium conductance and hyperpolarizing the dendrites. [less ▲]

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See detailEffect of drug candidates for the treatment of Alzheimer's disorber on hippocampus-dependent learning, glutamate receptors and mitochondria
Vignisse, Julie ULg; Steinbusch, H.W.M; Bolkunov, A. et al

Conference (2011, November 12)

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See detailEffect of dry ultra-fine reduction on physico-chemical properties of pea starch
Jacqmin, S.; Paquot, Michel ULg

in Food macromolecules and colloids adited by E-Dickinson and and D. Lorient, The royal Society of chemistry (1995)

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See detailEffect of drying and long term conservation on bioremediation properties of Rhodococcus erythropolis
Weekers, F.; Jacques, P.; Springael, D. et al

in Mededelingen van de Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen (Rijksuniversiteit te Gent) (1997), 62/4b

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See detailEffect of drying and long term conservation on bioremediation properties of Rhodococcus erythropolis.
Weekers, F.; Jacques, Ph.; Springael, D. et al

in Mededelingen - Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent (1994), 62(4b),

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See detailEffect of drying methods on physico-chemical and antioxidant properties of date fibre concentrates
Borchani, I.; Besbes, S.; Masmoudi, M. et al

in Food Chemistry (2011), 125

Effects of different drying methods (freeze-, oven- and sun-drying) on physico-chemical properties of date fibre concentrates (DFC) from three potential Tunisian cultivars were investigated. DFC had high ... [more ▼]

Effects of different drying methods (freeze-, oven- and sun-drying) on physico-chemical properties of date fibre concentrates (DFC) from three potential Tunisian cultivars were investigated. DFC had high contents of dietary fibre (71.01–93.46% dry matter), with high proportions of insoluble dietary fibre. Freeze dried DFC had the highest values of swelling, water holding and oil holding capacities. This drying method gave also the lightest DFC colour. Kentichi fibre produced by freeze-drying had the highest viscosity and the lowest bulkier particles. The present work assessed polyphenol content and antioxidant activity of DFC using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The highest polyphenol content was found for freeze dried DFC, contrary to radical scavenging activity which was not affected by drying methods. Results suggest that freeze dried DFC had the highest potential to be used as a functional ingredient in food products. [less ▲]

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See detailEffect Of Drying On Bioremediation Bacteria Properties
Weekers, F.; Jacques, P.; Springael, D. et al

in Applied Biochemistry and Biotechnology (1998), 70-2

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See detailEffect of drying on paedomorphosis in Triturus alpestris
Denoël, Mathieu ULg; Joly, Pierre; Poncin, Pascal ULg

Conference (2000)

Paedomorphosis consists in the retention of larval characters in adult individuals. It has been observed in several species of newts and salamanders. Optimality models were proposed to explain why it ... [more ▼]

Paedomorphosis consists in the retention of larval characters in adult individuals. It has been observed in several species of newts and salamanders. Optimality models were proposed to explain why it occurs in a large range of aquatic habitats. These models predict that paedomorphs can be advantaged in two situations as contrasted as favourable habitats (paedomorph advantage) or harsh habitats where the newts can benefit of delaying metamorphosis provided that competition with metamorphosed individuals is relaxed (best-of-a-bad-lot hypothesis). This last tactic implies that the habitats are stable enough to ensure the survival of individuals that can not leave the pond. In this respect, our aim was to describe behaviour of paedomorphic Alpine newts (Triturus alpestris) dealing with lake drying. We studied a population from an Alpine lake that splits into two parts during summer, one of them drying then totally. We analyzed age structures by means of skeletochronology and capture-marking-recapture. Paedomorphs and metamorphs showed similar age structures with sexual maturity occurring at 4-5 years, suggesting that most paedomorphs did not metamorphose. CMR data revealed that a great proportion of the paedomorphs marked in the drying part of the lake did not metamorphose and survived to drying. We also carried out laboratory experiments using two-compartiments aquaria to simulate lake drying. These experiments proved that paedomorphic newts could cross terrestrial habitats to reach a pond. Thus paedomorphic newts can migrate on land while keeping their larval attributes rather than metamorphosing when their pond is drying up. Such a tactic appears adaptive because polymorphism in such a deep lake reduces competition between individuals (pers. obs.). [less ▲]

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See detailEffect of duodenal ornithine infusion in cows on milk production and blood plasma constituents at two levels of feeding
Teller, E.; Roets, E.; Godeau, Jean-Marie ULg et al

in Journal of Animal Physiology & Animal Nutrition (1988), 59

An ornithine solution, corresonding to 36.1 g ornithine/d, or an equivalent blank solution were each continuously infused into the duodenum of two lactating cows of the Friesian breed. The cows were fed ... [more ▼]

An ornithine solution, corresonding to 36.1 g ornithine/d, or an equivalent blank solution were each continuously infused into the duodenum of two lactating cows of the Friesian breed. The cows were fed either on a high energy and high protein diet (110% of energy and protein requirements) or a low energy and low protein diet (75% of requirements). Milk composition and milk yield were recorded, and blood plasma contents of amino acids, glucose, growth hormone (GH) and insulin were analysed. The procedure was repeated 1 year later. Ornithine had no effect on milk yields or blood components when thehigh energy and high protein diets were given. On the contrary, it significantly increased milk, lactose, and fat production with the low energy and low protein diets, while milk protein secretion was slightly affected. The levels of nonessential and essential amino acids in blood plasma were enhanced, except for histidine and methionine. The latter amino acids likely were limiting for milk protein synthesis. An increase in glucose supply in the underfed animals likely occured after ornithine administration, since there was a significant increase of plasma glucose and protein levels and total lactose production. Ornithine infusion increased the GH secretion only when the low energy and low protein diets were given. It was concluded that infusion of the non-casein amino acid ornithine into the duodenum increases milk secretion in underfed cows, and that GH likely is involved in this phenomen. [less ▲]

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See detailEffect of duration and temperature of previous vacuum-packed storage on the microbiological quality of Belgian Blue meat packed in high-oxygen atmosphere
Didimo Imazaki, Pedro Henrique ULg; Tahiri, Assia ULg; Daube, Georges ULg et al

Poster (2012, September 05)

The aim of this experiment was to study the effect of duration and temperature of previous vacuum-packed (VP) storage on the microbiological quality of Belgian Blue (BB) beef packed in high-oxygen ... [more ▼]

The aim of this experiment was to study the effect of duration and temperature of previous vacuum-packed (VP) storage on the microbiological quality of Belgian Blue (BB) beef packed in high-oxygen atmosphere. VP striploins from bulls (B) and cows (C) were stored at −1 °C and +4 °C for up to 80 days. These meats were subsequently repackaged under modified atmosphere (MA) – 70 % O2/30 % CO2 – at different times, and stored 2 d at +4 °C and 5 d at +8 °C. The average initial counts in VP meats were 3.6 log CFU/cm² (B) and 2.7 log CFU/cm² (C) for total viable count (TVC) at +22 °C; < 2.0 log CFU/cm² (B and C) for lactic ac id bacteria (LAB) at +22 °C; 1.1 log CFU/cm² (B) and 1.3 log CFU/cm² (C) for Enterobacteriaceae at +30 °C and < 1.0 log CFU/cm² (B and C) for Pseudomonas spp. and Brochothrix thermosphacta. During the first 40 days of VP storage, temperature had a striking influence on microbial growth. The maximum count differences between storage temperatures were obtained at the 20th day of storage: 2.7 log CFU/cm² (B) and 2.9 log CFU/cm² (C) for TVC, 4.0 log CFU/cm² (B and C) for LAB and 3.6 log CFU/cm² (B and C) for Enterobacteriaceae. The difference in TVC between temperatures at the 20th day tended to disappear once the meats were repacked under MA and stored during seven days. Conversely, the difference in LAB and Enterobacteriaceae counts tended to be maintained after MA repackaging, showing that duration and temperature of VP storage had influence on microbiological quality of BB meat subsequently stored in high-oxygen atmosphere. Moreover, chilling at temperatures very close to the freezing point of meat during VP storage, which has already showed innumerous advantages for physicochemical quality of meat, was capital to maintain the microbiological quality of BB fresh meat during subsequent MA-packed storage. [less ▲]

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See detailEffect of duration and temperature of previous vacuum-packed storage on the oxidative stability of Belgian Blue meat packed in high-oxygen atmosphere
Didimo Imazaki, Pedro Henrique ULg; Tahiri, Assia ULg; Thimister, Jacqueline ULg et al

Poster (2012, August 13)

The aim of this experiment was to study the effect of duration and temperature of previous vacuum-packed (VP) storage on the oxidative stability of Belgian Blue meat packed in high-oxygen atmosphere. VP ... [more ▼]

The aim of this experiment was to study the effect of duration and temperature of previous vacuum-packed (VP) storage on the oxidative stability of Belgian Blue meat packed in high-oxygen atmosphere. VP striploins from bulls and cows were stored at −1 °C and +4 °C for up to 80 days and analyzed. These same meat samples were also repackaged under modified atmosphere (MA) – 70 % O2/30 % CO2 – at different times, stored 2 d at +4 °C and 5 d at +8 °C, and then analyzed. Meat from cows presented a lower loss of redness than meat from bulls. A low lipid oxidation was observed in VP samples, but an increase of lipid oxidation took place after MA repackaging. Meat from cows presented a higher -tocopherol content. A decrease of α-tocopherol content during storage was observed as well. The fat content was also higher in meat from cows than in meat from bulls. The duration and temperature of vacuum-packed storage influenced the sensitivity of Belgian Blue beef to pigment and lipid oxidation during subsequent high-oxygen storage. [less ▲]

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See detailEffect of dynamic left ventricular dyssynchrony on dynamic mitral regurgitation in patients with heart failure due to coronary artery disease
LANCELLOTTI, Patrizio ULg; STAINIER, Pierre-Yves ULg; LEBOIS, Florence ULg et al

in American Journal of Cardiology (2005), 96(9), 1304-1307

In patients with heart failure, exercise-induced increases in mitral regurgitation (MR), which convey a poor prognosis, are related to the dynamic distortion of mitral valve geometry. It was hypothesized ... [more ▼]

In patients with heart failure, exercise-induced increases in mitral regurgitation (MR), which convey a poor prognosis, are related to the dynamic distortion of mitral valve geometry. It was hypothesized that dynamic MR may also be related to intermittent changes in left ventricular synchronicity during exercise. (c) 2005 Elsevier Inc. All rights reserved. [less ▲]

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