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See detailDevelopment Of Real-Time Rt-Pcr Assay For Detection Of Prunus Necrotic Ringspot Virus In Fruit Trees
Marbot, Sophie; Vendrame, Marina; Huwaert, Astrid et al

in Plant Disease (2003), 87(11), 1344-1348

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See detailDevelopment of recombinant stable house dust mite allergen Der p 3 molecules for component-resolved diagnosis and specific immunotherapy.
Bouaziz, Ahlem; Walgraffe, David; Bouillot, Celine et al

in Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (2014)

BACKGROUND: The allergen Der p 3 is underrepresented in house dust mite (HDM) extracts probably due to autolysis. Recombinant stable molecule of the allergen is thus needed to improve the diagnosis of ... [more ▼]

BACKGROUND: The allergen Der p 3 is underrepresented in house dust mite (HDM) extracts probably due to autolysis. Recombinant stable molecule of the allergen is thus needed to improve the diagnosis of allergy and the safety and efficacy of immunotherapy. OBJECTIVE: The current study reports the immunological characterization of two recombinant molecules of the HDM allergen Der p 3 as useful tools for diagnosis and immunotherapy. METHODS: Recombinant mature (rDer p 3) and immature (proDer p 3) Der p 3 and their corresponding S196A mutants were produced in Pichia pastoris and purified. The stability, IgE-binding capacity and allergenicity of the different proteins were analyzed and compared with those of the major mite allergen Der p 1 used as a reference. Additionally, the immunogenicity of the different allergens was evaluated in a murine model of Der p 3 sensitization. RESULTS: Compared to the IgE reactivity to recombinant and natural Der p 3 (nDer p 3), the mean IgE binding of patient's sera to rDer p 3-S196A (50 %) was higher. The poorly binding to nDer p 3 or rDer p 3 was due to autolysis of the allergen. Contrary to Der p 3, proDer p 3 displayed very weak IgE reactivity, as measured by sandwich ELISA and competitive inhibition, RBL degranulation and human basophil activation assays. Moreover, proDer p 3 induced a TH 1-biased immune response that prevented an allergic response in mice but retained Der p 3-specific T cell reactivity. CONCLUSION: rDer p 3-S196A should be used for the diagnosis of HDM allergy elicited by Der p 3, and proDer p 3 may represent a hypoallergen of Der p 3. This article is protected by copyright. All rights reserved. [less ▲]

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See detailDevelopment of response models for optimising HPLC methods
Dewé, Walthère ULg; Marini Djang'Eing'A, Roland ULg; Chiap, Patrice ULg et al

in Chemometrics and Intelligent Laboratory Systems (2004), 74(2), 263-268

For optimizing a liquid chromatographic method, direct modeling some responses like minimum resolution is sometimes difficult to perform. By modeling the retention time at apex and the times associated to ... [more ▼]

For optimizing a liquid chromatographic method, direct modeling some responses like minimum resolution is sometimes difficult to perform. By modeling the retention time at apex and the times associated to 13.4% of the peak height, it is possible to obtain estimations of these responses using an adequate cascade of equations, these equations being the definitions of these responses. As a multi-criteria decision has to be taken in most of these optimizations, a Derringer's function can be defined for each response of interest and a overall desirability, on which the final is taken, can be derived. In addition, the chromatogram in any experimental point can be predicted. (C) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailDevelopment of riveting technology through an analysis of Belgian patents (1830-1940)
Collette, Quentin; Wouters, Ine; de Favereau, Corentin et al

in Jasienko, Jerzy (Ed.) Proceedings of the International Conference on Structural Analysis of Historical Constructions (SAHC 2012) (2012)

The development of riveting technology in iron and steel structures in the 19th and 20th centuries were highly promoted by patents. Although riveting was widely used in buildings and civil engineering ... [more ▼]

The development of riveting technology in iron and steel structures in the 19th and 20th centuries were highly promoted by patents. Although riveting was widely used in buildings and civil engineering structures, little information about the development of this technology – how rivets were manufactured, various installation techniques – is available in literature. This paper discusses a database of patents related to riveting technology that was created to better understand the innovations in riveted connections and their technological evolution. The database includes patents relating to rivets registered in Belgium between 1830 and 1940. Belgian patents are a good proxy for inventiveness in riveting technology in this period, in industrialized countries generally, not only Belgium. As one of the most industrialized countries, Belgium attracted inventors from many countries seeking to register their inventions: foreign inventions represented approximately 75% of all patents. Breakthroughs by important Belgian, French, British, and American inventors/companies are represented in this source. A methodology based on both quantitative and qualitative analyses was implemented. The quantitative analysis dealt with the entire database and explored several key parameters, for example, the subject of the patent and the patenting organization/person. The database also permitted qualitative analyses; specifically, it helped enlarge our understanding of pioneering inventors and their inventions, in the important categories of rivet manufacturing (1855-1870) and installation techniques (1895-1910). As background for the conservation and renovation of historic iron and steel structures, it is vital to have an understanding of the know-how that was available over time. This kind of information is revealed in the patent record [less ▲]

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See detailDevelopment of routine dtection tests using PCR for certification
Chandelier, Anne; Cognet, Stephane; Marinho, Véra Lucia et al

in Med. Fac. Landbouw. Univ. Gent, 63/4b, 1998 (1998)

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See detailDevelopment of routine duplex RT-PCR tests for certification of fruit tree multiplication material
Massart, Sébastien ULg; Roussel, S.; Kummert, J. et al

Conference (2006)

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See detailDevelopment of routine duplex RT-PCR tests for certification of fruit tree multiplication material
Massart, Sébastien ULg; Roussel, S.; Kummert, J. et al

in Acta Horticulturae (2008), 781

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See detailDevelopment of routine RT-PCR ELOSA tests for fruit tree certification.
Kummert, Jean; Vendrame, Marina; Lepoivre, Philippe ULg et al

in Acta Horticulturae (2001), (550 (Vol. 1)),

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See detailDevelopment of routine RT-PCR tests for certification of fruit tree multiplication material.
Kummert, Jean; Vendrame, Marina; Steyer, Stephane et al

in Bulletin OEPP (2000), 30(3-4), 441-448

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See detailDevelopment of RT-PCR assays using fluorogenic 3 minor groove binder DNA probes for detection of fruit tree viruses.
Marbot, Sophie; Kummert, Jean; Vendrame, Marina et al

in Acta Horticulturae (2004), (657), 547-551

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See detailDevelopment of RT-PCR assays using fluorogenic 3' minor groove binder DNA probes for detection of fruit trees viruses
Roussel, S.; Kummert, J.; Salmon, M. et al

Conference (2004)

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See detailDevelopment of RT-PCR assays using fluorogenic-3 minor groove binder DNA probes for detection of fruit tree viruses.
Roussel, S.; Kummert, J.; Dutrecq, O. et al

in Acta Horticulturae (2004), 657(1),

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See detailDevelopment of RT-PCR tests for the routine detection of latent and ILAR viruses in fruit trees.
Marbot, Sophie; Kummert, Jean; Salmon, Marc ULg et al

in Plant Protection Science (2002), 38(Special 1),

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See detailDevelopment of SCAR markers and a semi-selective medium for the quantification of strains Ach 1-1 and 1113-5, two Aureobasidium pullulans potential biocontrol agents.
El-Hamouchi, Adil; Bajji, Mohammed; Friel, Damien et al

in Postharvest Biology & Technology (2008), 50(2-3), 216-223

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See detailDevelopment of semiochemical slow-release formulations as biological control devices
Heuskin, Stéphanie ULg; Lorge, Stéphanie ULg; Leroy, Pascal ULg et al

Poster (2012, July)

Semiochemicals have been widely considered within various integrated pest management (IPM) strategies. In the present work, two sesquiterpenoids, E-β-farnesene and E-β-caryophyllene, were formulated for ... [more ▼]

Semiochemicals have been widely considered within various integrated pest management (IPM) strategies. In the present work, two sesquiterpenoids, E-β-farnesene and E-β-caryophyllene, were formulated for their related properties as aphid enemy attractants. E-β-farnesene, the alarm pheromone of many aphid species, was also identified as a kairomone of aphid predators (Episyrphus balteatus De Geer (Diptera: Syrphidae)) and parasitoids (Aphidius ervi Haliday (Hymenoptera: Braconidae)). E-β-caryophyllene was identified as a potential component of the aggregation pheromone of the Asian ladybird, Harmonia axyridis Pallas, another aphid predator. The two products were purified from essential oils of Matricaria chamomilla L. (Asteraceae) and Nepeta cataria L. (Lamiaceae) for E-β-farnesene and E-β-caryophyllene, respectively. Natural and biodegradable slow-release formulations were then investigated in order to deliver these molecules on crop fields for a long period of time as biological control devices. Due to their sensitivity to oxidation, both sesquiterpenes needed to be protected from degradation. For this purpose, alginate – hydrophilic matrix with low oxygen permeability – was used as polymer for the formulations: the main objective was to deliver semiochemical substances in the air in a controlled way. Consequently, a careful selection of alginates was realised. Formulated beads showed different structural and encapsulation properties depending on various formulation factors. Alginate formulations were characterized by texturometry and by confocal microscopy in order to observe the distribution of semiochemicals in alginate network. The last step of alginate bead characterisation consisted in studying release rate of semiochemicals in laboratory-controlled conditions by optimised trapping and validated Fast-GC procedures. Finally, the efficiency of formulations as aphid predator (Syrphidae) and parasitoid (A. ervi) attractants was demonstrated by field trapping and olfactometry experiments. [less ▲]

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See detailDEVELOPMENT OF SHALLOW RESERVOIR IRRIGATION BY USING FLOODWATER FOR PADDY RICE CULTIVATION
Someth, Paradis; Kubo, N.; Ly, Sarann ULg et al

in Tanji, Hajime (Ed.) Proceedings of the Second International Symposium on Sustainable Development in the Mekong River Basin (2006, September 18)

The Tonle Sap Great Lake and the Mekong River in Cambodia have huge floodplain suitable for rice-based farming system and potential water resources for farming. Studies to exploit the flood and the water ... [more ▼]

The Tonle Sap Great Lake and the Mekong River in Cambodia have huge floodplain suitable for rice-based farming system and potential water resources for farming. Studies to exploit the flood and the water resources of the lake and the river have not been well conducted yet. Batheay irrigation system is located in the floodplain of the Tonle Sap River and the Mekong River. In this research, the irrigation system is studied as a model site for future development of the floodplain of the Tonle Sap Great Lake and the Mekong River. Batheay reservoir directly receives floodwater from the Mekong. It functions as both a reservoir and a paddy field. In wet season, dike around the Batheay reservoir prevents floodwater from entering the reservoir. Rainy season rice is grown inside the reservoir. After harvesting, gates around the reservoir are opened to receive floodwater. The water is stored for cultivating dry season rice outside the reservoir. In this work, we attempt to study water management of the Batheay irrigation system by analyzing water use pattern in the irrigation system and uncover effectiveness of the system. This study plays a vital role in the study of the Tonle Sap Great Lake system in our future research. [less ▲]

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