Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
See detailDesign Monopile Foundation of Offshore Wind Turbines
Mai, Anh Quang ULg

Master's dissertation (2012)

Design optimization is crucial to the development of the offshore wind turbine industry. This time consuming process is better to be done with a number of input parameters that is as short as possible ... [more ▼]

Design optimization is crucial to the development of the offshore wind turbine industry. This time consuming process is better to be done with a number of input parameters that is as short as possible. Whether the foundation pile part can be neglected in the design optimization process of an offshore wind turbine structure is a question need to be answer. In order to see the importance of the presence of the foundation pile in dynamic behavior of the whole structure, dimensions of the foundation pile must be determined basing on requirements in ultimate limit state and serviceability limit state in current design standards. Afterward, the differences in dynamic behavior between a fixed- at-seabed tower model and a tower with foundation model must be observed. Beam nonlinear Winkler Foundation model in addition to gapping and non-gapping behavior in pile-soil interface were used to model the foundation. With the chosen offshore wind turbine project of 7MW and 115m high to seabed, a foundation pile with a penetration length of 26m, diameter of 6m and wall thickness of 8cm had been found. The dynamic behavior of the two models showed that it was not on the safe side if the foundation was neglected in design optimization process. And that the internal damping of the soil was the most important factor in behavior of the structure. These results will be useful for reconsidering parameters in design optimization process of monopile offshore wind turbines as well as choosing suitable methods to solve dynamic equations in the optimization procedure. [less ▲]

Detailed reference viewed: 16 (3 ULg)
Full Text
Peer Reviewed
See detailDesign of a biofilm reactor comprising a metal structured packing for the production of lipopeptides by B. subtilis
Zune, Quentin ULg; Ongena, Marc ULg; Toye, Dominique ULg et al

Poster (2012, February 08)

Abstract : The design of a new single species biofilm bioreactor has been investigated. Bacillus subtilis S499 has been chosen as a model organism for the production of lipopetides. Nevertheless ... [more ▼]

Abstract : The design of a new single species biofilm bioreactor has been investigated. Bacillus subtilis S499 has been chosen as a model organism for the production of lipopetides. Nevertheless, considering the surface active properties for this kind of metabolite, processes based on submerged culture in stirred-tank bioreactor involve the use of important amount of antifoam and therefore downstream processes are tedious. In this work, an original process was developed with an experimental setting leading to the suppression of foam formation during the culture. B. subtilis S499 makes a biofilm on a stainless steel structured packing in the top of a bioreactor, nutrient and oxygen supply being carried out by the media recirculation as liquid film on the packing. Lipopeptides secreted by biofilm are accumulated in the liquid phase under the packing and can reach concentrations as high as 800 mg/l. The colonization of the packing by the biofilm has been monitored by X-ray tomography. [less ▲]

Detailed reference viewed: 119 (30 ULg)
Peer Reviewed
See detailDesign of a convective labscale dryer working either with superheated steam or air
Fraikin, Laurent ULg; Salmon, Thierry ULg; Léonard, Angélique ULg

in Proceedings of the 5th European Drying Conference (EuroDrying'2015) (2015)

Detailed reference viewed: 7 (1 ULg)
Full Text
Peer Reviewed
See detailDesign of a decision tool for hydromorphological restoration of water bodies in Walloon Region
Peeters, Alexandre ULg; Hallot, Eric ULg; Verniers, Gisèle et al

Poster (2010, May 05)

The pilot project WALPHY (LIFE + Environment LIFE07 ENV/B/000038, UE-funded) develops a structured approach aiming at improving hydromorphological quality of the upstream Meuse basin in order to achieve ... [more ▼]

The pilot project WALPHY (LIFE + Environment LIFE07 ENV/B/000038, UE-funded) develops a structured approach aiming at improving hydromorphological quality of the upstream Meuse basin in order to achieve the “good ecological status” required by the Water Framework Directive (WFD 2000/60). It began in January 2009 for a period of 5 years. River’s biological recovery needs the recovery of river’s structure and physical dynamics. Returning to "good ecological status" inevitably involves physical restoration of affected rivers. Physical component of aquatic environment acts as a limiting factor for their functioning. Three types of modification are key obstacles for river good ecological status: (i) fluxes alteration (discharge, sedimentation. . . ), (ii) forms alteration (uniform facies. . . ) and (iii) biotopes access alteration (lateral connections breaks, modification of the continuity upstream/ downstream. . . ). Therefore hydromorphology is needed to implement the WFD. To respond to this legal necessity, we develop a unique, useful and suitable methodology in Walloon Region to determine and schedule river physical quality restoration works. This methodology has been applied on 3 “risk water bodies”. The works are based on two axes : longitudinal continuity and transversal continuity. The two first selected water bodies (Bocq river, eastern tributary of the Meuse) seem to be convenient for the restoration works which concern the longitudinal continuity due to the presence of dams and other obstacles usually between 1 and 3 m high. These works consist in dam management (weir removal or fish passage) taking into account hydromorphological (bedload transport) and biological (invertebrate or fish species free movement) impacts. The third water bodies (Eau Blanche river, western tributary of the Meuse) presents straightened rivers with artificial banks, witch consequently own bad connections between the stream and its floodplain. This water bodies should therefore be appropriated for the works based on the transversal continuity recovery. They consist in enhancing straightened river channels, restoring meanders or bank restoration. The first river restoration works will take place from summer 2010. A monitoring has already been undertaken to draw up the situation before the restoration works, witch will be able to compare to the situations during and after works. In addition, a natural site has been chosen to enable any comparison with the intervention sites. The geomorphological monitoring is based on physical and sedimentological parameters (substrate cartography, velocity measures, stream channel DEM, topography and sedimentological index). The ecological monitoring consists in analysing the physical and chemical parameters (turbidity, suspended sediment load). It also analyses the biological quality through 3 indicators: macrophytes, macroinvertebrates and fishes. Among other, a microhabitats method has been improved and applied on 3 intervention sites and 1 natural site. The microhabitats cartography results from both the flow velocity cartography and the substrate cartography. With this method, the physical quality of each intervention site could be compared with the natural site and above all with the future situation. Our method is also useful to inventory precisely invertebrates and to characterise fish habitats. [less ▲]

Detailed reference viewed: 33 (8 ULg)
See detailDesign of a foil-coiled inductor for the heating of steel wires
De Coster, Jeroen; Deliège, Geoffrey ULg; Henrotte, François et al

in Computation in Electromagnetics, CEM 2002, Bournemouth, UK, 8-11 April, 2002 (2002)

Detailed reference viewed: 22 (0 ULg)
Full Text
Peer Reviewed
See detailDesign of a foil-coiled inductor for the heating of steel wires
De coster, Jeroen; Deliège, Geoffrey ULg; Henrotte, François et al

in IEE Proceedings - Science, Measurement & Technology (2002), 149(5), 203-206

Detailed reference viewed: 20 (0 ULg)
Full Text
Peer Reviewed
See detailDesign of a fungal biofilm reactor for recombinant protein production from Aspergillus oryzae
Zune, Quentin ULg; Delepierre, Anissa ULg; Bauwens, Julien ULg et al

Poster (2014, September 07)

Fungi are microorganisms exhibiting high secretive power of various metabolites and have the ability to perform post-translational modifications during protein synthesis. In the field of fermentation ... [more ▼]

Fungi are microorganisms exhibiting high secretive power of various metabolites and have the ability to perform post-translational modifications during protein synthesis. In the field of fermentation industry, they are ideal hosts for secondary metabolites and recombinant protein production. At the industrial-scale, equipments usually required for solid-state or submerged fermentation of filamentous fungi have demonstrated their limitations in terms of productivity, mass transfers or products recovery (1, 2). Recently, fungal biofilm reactors were designed to combine advantages from submerged and solid-state culture and reveal their usefulness for greater secondary metabolites production relative to submerged culture conditions (3). In our work, we propose the design of a fungal biofilm reactor for a recombinant protein production from an Aspergillus oryzae strain containing a GFP reporter gene system under the control of a promoter specifically induced in solid-state conditions. The fungal biofilm reactor is composed of a metal structured packing, having the function of inert support for biofilm growth, immerged or aspersed by a liquid medium. Whereas recombinant protein production is not significantly different at the flask-scale between submerged and biofilm conditions, productivity is higher in the submerged conditions at the bioreactor-scale. Presence of recombinant proteins entrapped in the biofilm matrix highlights a diffusion constraint and a lower mass transfer in our fungal biofilm reactor. However, persistence of a free liquid biomass of low viscosity and fungal biomass retention on the support are attractive for the implementation of a continuous process in our fungal biofilm reactor. Further studies will consider a 2-D proteomic comparison of the extracellular medium from fungal biofilm reactor and submerged culture conditions in order to better understand proteins secretion and identify over-expressed proteins in biofilm conditions. [less ▲]

Detailed reference viewed: 120 (14 ULg)
See detailDesign of a linear transverse flux actuator
Vande Sande, Hans; Deliège, Geoffrey ULg; Hameyer, Kay et al

in 15th International conference on electrical machines (ICEM), n°152, Brugge, Belgium, August 25-28, 2002 (2002)

Detailed reference viewed: 21 (2 ULg)
See detailDesign of a linear transverse flux actuator for fast positioning
Vande Sande, Hans; Deliège, Geoffrey ULg; Hameyer, Kay et al

in Compumag, Evian, France, July 2-5, 2001 (2001)

Detailed reference viewed: 49 (0 ULg)
Full Text
Peer Reviewed
See detailDesign of a microwave array hyperthermia applicator with a semicircular reflector
V Sabariego, Ruth ULg; Landesa, Luis; Obelleiro, Fernando

in Medical & Biological Engineering & Computing (1999), 37(5), 612-617

The design of a hyperthermia applicator for heating biological tissues is presented in which the applicator consists of an array antenna surrounded by a perfect electrically conducting reflector. The heat ... [more ▼]

The design of a hyperthermia applicator for heating biological tissues is presented in which the applicator consists of an array antenna surrounded by a perfect electrically conducting reflector. The heat hazard to superficial tissues is reduced by the introduction of a dielectric protecting layer over them. A method of moments formulation is applied to approximate the electric field within the biological medium and a closed form expression is presented for the electromagnetic coupling problem, which enables an optimisation procedure to be performed. The applicator enhances both penetration and focusing: deep tumours, close to the bone region, are heated and the percentage of biologically healthy tissue exposed to a specific absorption rate (SAR) hazard level diminishes by 53.8%. [less ▲]

Detailed reference viewed: 22 (2 ULg)
Full Text
Peer Reviewed
See detailDesign of a morphological moving object signature and application to human identification
Barnich, Olivier ULg; Van Droogenbroeck, Marc ULg

in International Conference on Acoustics, Speech, and Signal Processing (ICASSP 2009) (2009, April)

Many computer vision systems try to infer semantic information about a video scene content by looking at the time series of the silhouettes of the moving objects. This paper proposes a new inter-frame ... [more ▼]

Many computer vision systems try to infer semantic information about a video scene content by looking at the time series of the silhouettes of the moving objects. This paper proposes a new inter-frame feature set (signature) based on piecewise surfacic descriptions of binary silhouettes. It captures the dynamics of moving objects and compacts it into a robust set of features suitable for classification. To assess its ability to represent motion information, we use it to build a complete gait recognition algorithm that we test on a database of 21 different subjects. To highlight the efficiency of our signature, we use frontal views instead of side views of persons, which is less discussed in literature and is considered to be harder as the movement of legs is not visible. In that context, the high recognition rates obtained (over 95% of correct identifications) proves that our signature is appropriate to describe moving objects. [less ▲]

Detailed reference viewed: 173 (7 ULg)
Full Text
Peer Reviewed
See detailDesign Of A New Class Of Amphipathic Helical Peptides For The Plasma Apolipoproteins That Promote Cellular Cholesterol Efflux But Do Not Activate Lcat
Labeur, C.; Lins, Laurence ULg; Vanloo, B. et al

in Arteriosclerosis, Thrombosis, and Vascular Biology (1997), 17(3), 580-8

Amphipathic helical peptides represent the lipid-binding units of the soluble plasma apolipoproteins. Several synthetic peptide analogues have been designed to mimic such structures and have been used to ... [more ▼]

Amphipathic helical peptides represent the lipid-binding units of the soluble plasma apolipoproteins. Several synthetic peptide analogues have been designed to mimic such structures and have been used to unravel some of the mechanisms involved in the physiological function of the apolipoproteins, including lipid binding, LCAT activation, and enhancement of cholesterol efflux from lipid-laden cells. A series of novel synthetic peptides, named ID peptides, was modeled on the basis of the structural properties common to the amphipathic helices of apolipoprotein (apo) A-I. In these new peptides, however, the segregation between hydrophobic and hydrophilic faces of the helices is more pronounced than in apoA-I, so that the surface of the hydrophobic and hydrophilic faces of the amphipathic helices is equal. Moreover, there are fewer negatively charged residues in the center of the hydrophilic face of the helical peptides. Most charged amino acids are located along the edge of the helix and are susceptible to forming salt bridges with residues of an antiparallel helix, such as around a discoidal phospholipid/peptide complex. The physicochemical characteristics of these peptides and their complexes with phospholipids were compared with those of the 18A peptide and its lipid/peptide complex. All ID peptides bind dimyristoylphosphatidylcholine vesicles more rapidly than the 18A peptide to yield discoidal peptide/phospholipid complexes of comparable size. The alpha-helical content of the lipid-free ID peptides is close to that of the 18A peptide and increases slightly on lipid binding. The stability of the ID and 18A peptides and of the phospholipid/peptide complexes against guanidinium hydrochloride denaturation is higher than that of lipid-free and lipid-bound apoA-I. LCAT activation by the 18A/phospholipid/cholesterol complexes equals that of apoA-I/ phospholipid/cholesterol complexes, whereas none of the ID peptides tested is able to activate LCAT to a significant extent. Incubation of the peptide/phospholipid complexes with lipid-laden macrophages induces cellular cholesterol efflux and incorporation of cholesterol into the complexes. The cholesterol efflux capacity of the peptide/phospholipid complexes is comparable among the peptides and higher than that of apoprotein/phospholipid complexes. In conclusion, although the amphipathicity of the new peptides is higher than that of the 18A model peptide, the lack of LCAT activation by the ID peptides suggests that an enhanced segregation of the hydrophobic and hydrophilic residues, equal magnitude of hydrophobic and hydrophilic faces of the helix, and the absence of negatively charged residues in the central part of the hydrophilic face might account for the lack of LCAT activity of these peptides. These parameters do not affect the capacity of the peptide/phospholipid complexes to promote cellular cholesterol efflux. [less ▲]

Detailed reference viewed: 25 (0 ULg)
Peer Reviewed
See detailDesign of a New Type of Semi-Submersible Structure
Marchal, Jean ULg

in ASME Journal (American Society of Mechanical Engineers) (1987)

Detailed reference viewed: 11 (0 ULg)
Full Text
Peer Reviewed
See detailDesign of a novel covert SSVEP-based BCI
Lesenfants, Damien ULg; Partoune, Nicolas; Soddu, Andrea ULg et al

in Proceedings of the 5th International Brain-Computer Interface Conference 2011 (2011, September 22)

Detailed reference viewed: 141 (14 ULg)
Full Text
Peer Reviewed
See detailDesign of a reliable processing pipeline for the non-intrusive measurement of feet trajectories with lasers
Pierard, Sébastien ULg; Azrour, Samir ULg; Van Droogenbroeck, Marc ULg

in International Conference on Acoustics, Speech, and Signal Processing (ICASSP) (2014, May)

Reliable measurements of feet trajectories are needed in some applications, such as biomedical applications. This paper describes the data processing pipeline used in GAIMS, which is a non-intrusive ... [more ▼]

Reliable measurements of feet trajectories are needed in some applications, such as biomedical applications. This paper describes the data processing pipeline used in GAIMS, which is a non-intrusive system that measures feet trajectories based on multiple range laser scanners. Our processing pipeline relies on a new tracking paradigm, and it is based on two innovative algorithms: the first algorithm localizes the feet directly from the observed point cloud without any clustering, and the other algorithm identifies the feet. After reviewing the various types of noise affecting the point cloud, this paper explains the limitations of the classical processing approach and gives an overview of our new pipeline. The effectiveness of the proposed approach is established by discussing the results that have been obtained in several studies based on GAIMS. [less ▲]

Detailed reference viewed: 101 (36 ULg)
Peer Reviewed
See detailDesign of a Self Compacting Repair Mortar to be applied under concrete slabs and floors
Flamant, St; Courard, Luc ULg

in Marchand, J.; Bissonnette, B.; Gagné, R. (Eds.) et al Advances in Concrete through Science and Engineering (2006, September)

Detailed reference viewed: 70 (4 ULg)
Full Text
See detailDesign of a single-species biofilm reactor based on metal structured packing for the production of high added value biomolecules
Zune, Quentin ULg

Doctoral thesis (2015)

Thesis summary In the last decade, numerous single-species biofilm reactors of various configurations have been implemented at lab and pilot scale for the production of chemicals and biological products ... [more ▼]

Thesis summary In the last decade, numerous single-species biofilm reactors of various configurations have been implemented at lab and pilot scale for the production of chemicals and biological products. Compared to their counterparts in submerged cultures, these processes benefit from the specific physiology of biofilms, i.e. high robustness of the microbial system, long-term activity, continuous implementation and low ratio size / productivity. However, the risks of biofouling and the lack of analytical tools for the control and the monitoring of biofilms are obstacles for scale-up strategies. Up to now, single-species biofilm reactors have been mainly confined to the production of metabolites ranging from low (bulk chemicals) to medium (fine chemicals) added values. In this way, there is a need to design efficient single-species biofilm reactors exhibiting good scalability potentials and intended for the production of high added value compounds. In this work, an experimental single-species biofilm reactor has been designed for the production of target molecules derived from metabolic pathways involved in biofilm physiology. On the basis of these criteria, three biological models having good abilities of biofilm formation and secretion performances were selected : - the gram positive bacterium Bacillus subtilis for the production of surfactin, a surface active metabolite involved in biofilm formation. - the filamentous fungus Trichoderma reesei for the production of hydrophobin (HFBII), a surface active protein (7kDa) involved in adhesion process of spores and mycelium on solid surface. - the filamentous fungus Aspergillus oryaze (engineered strain) for the production of a recombinant protein (Gla::GFP) under the control of the glaB promoter specifically activated in solid-state fermentation. The proposed experimental biofilm reactor has the configuration of a trickle-bed bioreactor. The agitation axis of a stirred tank reactor has been removed and replaced by a stainless steel structured packing filling the top of the vessel. The liquid medium, located in the bottom of the vessel is continuously recirculated on the packing element thanks to a peristaltic pump. An ascending air flow is performed above the liquid phase just under the packing element. This thesis reports the screening of the three biological models in the experimental biofilm reactor. The results include the characterization of process performances in terms of biofilm formation and secretion of the target molecule under different operating conditions. An original methodology based on high energy X-ray tomography has been developed to non-invasively visualize and quantify the biofilm colonization inside the packing element. This technique has highlighted that biofilm colonization and liquid phase distribution across the packing are strongly interrelated phenomena. The biofilm of B. subtilis occurring by cell aggregation preferentially developed on solid areas wetted by the liquid. Accordingly, optimal operating conditions improving liquid phase distribution have been defined for biofilm colonization. The fungal biofilm of A. oryzae and T. reesei occuring by cell filamentation equally colonize submerged and aerial surfaces of the packing element. Consequently, another configuration of biofilm reactor comprising a packing element totally immersed in the liquid medium has been investigated. The production yields of surfactin and hydrophobin in the experimental biofilm reactor are respectively 1.25 and 2.64 times greater than those of a submerged culture in a stirred tank reactor. This suggests that surface-active molecules involved in biofilm formation have a real interest for the design of single-species biofilm reactors. Although the Gla::GFP fusion protein is greater produced in the stirred tank culture, its integrity was preserved in the biofilm reactor despite the presence of proteases. This suggests that the quality and the stability of heterologous proteins produced in a fungal biofilm reactor are improved compared with a submerged culture. Finally, the implementation of the biofilm reactor has led to technological progresses including low energy consumption, no foam formation, continuous processing and simplification of downstream process operations. Further experiments should deepen the understanding of structured phenotypic heterogeneity impact on secretion performances in the biofilm reactor. These experiments should consider development of operating conditions allowing for the growth of a thin biofilm homogeneously distributed on the whole surface provided by the packing element in order to optimize nutrients and metabolites mass transfers. The scale-up and the continuous implementation of the process should be also investigated. [less ▲]

Detailed reference viewed: 124 (53 ULg)