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See detailEffect of groundnut cake substitution by glandless cottonseed kernels on broilers production: animal performance, nutrient digestibility, carcass characteristics and fatty acid composition of muscle and fat
Diaw, Mamadou Tandiang; Dieng, Abdoulaye; Mergeai, Guy ULg et al

in International Journal of Poultry Sciences (2010), 9(5), 473-481

A study has been conducted with broilers to assess, during the rainy season, the effects of groundnut cake substitution by glandless Cottonseed Kernel (CSK), at levels of 0, 25, 50 and 75%. The ... [more ▼]

A study has been conducted with broilers to assess, during the rainy season, the effects of groundnut cake substitution by glandless Cottonseed Kernel (CSK), at levels of 0, 25, 50 and 75%. The substitution improved linearly feed intake and animal growth, as well as carcass component weights and allometric parameters. The CSK increased the C18:2 n-6 to C18:1 n-9 ratio, as well in diet as in meat and subcutaneous fat. In order to explain the observed performances, the possibility is considered that broilers used preferentially C18:2 n-6 fatty acids for their metabolism. Complete glandless cottonseed kernels are probably highly valuable for broilers production in warm and wet conditions. [less ▲]

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See detailThe effect of growth at low temperature on the activity and expression of the uncoupling protein in Acanthamoeba castellanii mitochondria.
Jarmuszkiewicz, W.; Antos, N.; swida, A. et al

in FEBS Letters (2004), 569

Mitochondria of amoeba Acanthamoeba castellanii, a non-photosynthetic soil amoeboid protozoon, possess an uncoupling protein (AcUCP) that mediates free fatty acid-activated proton re-uptake dissipating ... [more ▼]

Mitochondria of amoeba Acanthamoeba castellanii, a non-photosynthetic soil amoeboid protozoon, possess an uncoupling protein (AcUCP) that mediates free fatty acid-activated proton re-uptake dissipating the proton electrochemical gradient built up by respiration. The present study provides the first evidence that UCP could be a cold response protein in unicellulars. In mitochondria isolated from an amoeba batch culture grown temporarily at low temperature (6 degrees C), the content of AcUCP was increased and correlated with an increase in the linoleic acid (LA)-stimulated UCP-mediated carboxyatractyloside-resistant state 4 respiration, as compared to a control culture (routinely grown at 28 degrees C). Moreover, the cytochrome pathway activity was found to be insensitive to the cold exposure of amoeba cells, as indicated by respiration and membrane potential measurements as well as by an absence of change in the adenine nucleotide translocator and cytochrome oxidase expression levels. Furthermore, in mitochondria from the low-temperature-grown cells, at fixed LA concentration, the increased contribution of AcUCP activity to total mitochondrial phosphorylating respiration accompanied by lower coupling parameters was found, as was confirmed by calculation of this contribution using ADP/O measurements. [less ▲]

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See detailEffect of growth rate and radial position on the natural durability of Douglas-fir
Pollet, Caroline; Henin, Jean-Marc; Jourez, Benoît ULg et al

Conference (2012, July 11)

In terms of natural durability, Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) fame owns to the favourable characteristics of centuries-old trees harvested in old-growth North American forests. The ... [more ▼]

In terms of natural durability, Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) fame owns to the favourable characteristics of centuries-old trees harvested in old-growth North American forests. The properties of wood coming from plantations harvested between 50 and 100 years-old are likely to be different. In such stands, plantation density and thinning intensity may have a large impact on the trees growth rate. Since this parameter is known to affect some properties of the wood, it was decided to assess to what extent an increase in Douglas fir growth rate affects the natural durability of its wood. This issue is indeed poorly documented in the scientific literature. This parameter was evaluated on standardized heartwood specimens taken from 60 trees originating from 10 stands in Wallonia (Southern Belgium). In all these stands, the average girth of the trees ranged between 140 and 160cm, whilst their age (from plantation) ranged from 38 to 66 years old: These stands are thus representative of very contrasted silvicultural management practices. In terms of individual growth, the Mean Ring Width of the trees ranges between ca 3 and 7mm. Globally, 600 tests specimens were taken from two radial positions in the heartwood of each tree. Half of the specimens were taken in the heartwood at the border of the sapwood; the other half encloses the 20 years old ring, counting from the pith. The mass losses caused by the wood decaying fungus Poria placenta were assessed according to Cen/ts 15083-1 (2005).The natural durability of the wood is discussed as affected by sites, trees, radial positions in tree and tree growth rate. [less ▲]

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See detailEffect of growth rate on the natural durability of Douglas-fir in western Europe
Pollet, Caroline; Henin, Jean-Marc; Hebert, Jacques ULg et al

in Canadian Journal of Forest Research = Journal Canadien de la Recherche Forestière (2013), 43(10), 891-896

Douglas-fir is a relatively recent species in the central European forest landscape. It is not clear how its natural durability is influenced by silviculture. We therefore investigated how the natural ... [more ▼]

Douglas-fir is a relatively recent species in the central European forest landscape. It is not clear how its natural durability is influenced by silviculture. We therefore investigated how the natural durability of the (Pseudotsuga menziesii (Mirb.) Franco) Douglas-fir varies according to tree growth rate; subsequently, the influence of the nature of the wood (juvenile or mature) and radial position was also analyzed. For this purposes, a total of 60 trees grown in Wallonia (Southern Belgium) was felled: these consisted of 6 trees in each of 10 stands where the average tree girth was approximately 150 cm. Since the age of these plantations ranged from 40 to 69 years, the radial tree growth rate ranged between approximately 3 and 7 mm. In all the trees, standardized heartwood test specimens were taken from different radial positions in the butt log. The mass losses caused by the fungi Poria placenta and Coniophora puteana were assessed according to CEN/TS 15083-1 (2005). Poria placenta exhibited a higher decaying efficiency than C. puteana, leading to classify Douglas-fir wood as slightly durable (class 4). No difference was observed between the durability against P. placenta of juvenile and mature wood. Similarly, growth rate seemed to have no direct impact on durability. Durability was, however, significantly correlated with wood density. The effect of site and tree on the natural durability of the wood was also examined. [less ▲]

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See detailEffect of haemodialysis on acoustic shear wave propagation in the skin.
Uhoda, I.; Petite, Louicette ULg; Krzesinski, Jean-Marie ULg et al

in Dermatology : International Journal for Clinical & Investigative Dermatology (2004), 209(2), 95-100

BACKGROUND: Diverse skin alterations may develop in patients under chronic haemodialysis. Among them, signs of premature photo-ageing have been described. AIM: To assess alterations in the physical ... [more ▼]

BACKGROUND: Diverse skin alterations may develop in patients under chronic haemodialysis. Among them, signs of premature photo-ageing have been described. AIM: To assess alterations in the physical properties of skin consistent with ageing effects and with cutaneous fluid exchanges during haemodialysis sessions. METHODS: In the first part of the study, 45 haemodialysed patients were compared to 45 age-, sex- and body-mass-index-matched healthy subjects. In the second part of the study, skin of 30 haemodialysed patients was assessed immediately before and after a haemodialysis session. The speed of ultrasound shear wave propagation was measured in each subject. Series of 16 multidirectional resonance running time measurements (RRTM) were performed on the forehead and/or the volar forearm. They were averaged for each subject. The corresponding intra-individual coefficients of variation were calculated as an estimate of the skin mechanical anisotropy. RESULTS: In both haemodialysed patients and their matched controls, RRTM values were significantly higher on the forearms than on the forehead. By contrast, no significant difference was found in RRTM values that could be ascribed to chronic haemodialysis. However, RRTM values were significantly increased as an immediate and probably transient effect of haemodialysis sessions. In healthy subjects, ageing was associated with increased RRTM values. CONCLUSION: Chronic haemodialysis does not appear to influence significantly the functional expression of the dermal ageing process. Subtle fluid movements occurring in the skin during haemodialysis sessions can be assessed by measuring non-invasively the speed of ultrasound shear wave propagation in the skin. [less ▲]

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See detailEffect of harvest time on seed oil and protein contents and compositions in the oleaginous gourd Lagenaria siceria (Molina) Standl
Loukou, Ahou; Lognay, Georges ULg; Barthelemy, Jean-Paul ULg et al

in Journal of the Science of Food and Agriculture (2011), 91(11), 2073-2080

BACKGROUND: The stage of fruit ripeness at the time of harvest determines the final quality of ripe fruit. In this study, changes in the chemical composition of seed kernels from the oleaginous gourd ... [more ▼]

BACKGROUND: The stage of fruit ripeness at the time of harvest determines the final quality of ripe fruit. In this study, changes in the chemical composition of seed kernels from the oleaginous gourd Lagenaria siceraria (Molina) Standl. during maturation were evaluated to determine the best time to harvest the berries. Two cultivars (round and oval berry) were studied at three maturation stages (30 and 50 days after fruit set (DAFS) and complete plant whiteness (CPW)). [less ▲]

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See detailEffect of head-to-head addition in vinyl acetate controlled radical polymerization: why is Co(acac)2-mediated polymerization so much better?
Morin, Aurélie N.; Detrembleur, Christophe ULg; Jérôme, Christine ULg et al

in Macromolecules (2013), 46(11), 4303-4312

The controlled polymerization of vinyl acetate has been recently achieved by several techniques, but PVAc with targeted Mn and low dispersity up to very high monomer conversions and high degrees of ... [more ▼]

The controlled polymerization of vinyl acetate has been recently achieved by several techniques, but PVAc with targeted Mn and low dispersity up to very high monomer conversions and high degrees of polymerization was only obtained with Co(acac)2 as controlling agent in the so-called CMRP, a type of organometallic mediated radical polymerization (OMRP). Other techniques (including ATRP, ITP, TERP, and RAFT/MADIX) have shown a more or less pronounced slowdown in the polymerization kinetics, which was attributed to the higher strength of the C−X bond between the radical PVAc chain and the trapping agent (X) in the dormant species and to a consequent slower reactivation after a less frequent head-to-head monomer addition. The reason for the CMRP exception is clarified by the present contribution. First, a detailed investigation by 1H, 13C and multiplicity-edited HSQC and DEPT-135 NMR of the PVAc obtained by CMRP, in comparison with a regular polymer made by free radical polymerization under the same conditions, has revealed that Co(acac)2 does not significantly alter the fraction of head-to-head sequences in the polymer backbone and that there is no accumulation of Co(acac)2-capped chains with a head-to-head ω end. Hence, both dormant chains (following the head-to-head and the head-to-tail monomer additions) must be reactivated at similar rates. A DFT study shows that this is possible because the dormant chains are stabilized not only by the C−Co σ bond but also by formation of a chelate ring through coordination of the ω monomer carbonyl group. The head-to-head dormant chain contains an inherently stronger C−Co bond but forms a weaker 6-membered chelate ring, whereas the weaker C−Co bond in the head-to-tail dormant chain is compensated by a stronger 5-membered chelate ring. Combination of the two effects leads to similar activation enthalpies, as verified by DFT calculations using a variety of local, gradient-corrected, hybrid and “ad hoc” functionals (BPW91, B3PW91, BPW91*, M06 and M06L). While the BDE(C−X) of model H-VAc−X molecules [X = Cl, I, MeTe, EtOC(S)S and Co(acac)2] are functional dependent, the BDE difference between head-to-head and head-to-tail dormant chain models is almost functional insensitive, with values of 5−9 kcal/mol for the ATRP, ITP and TERP models, 3−6 for the RAFT/MADIX model, and around zero for CMRP. [less ▲]

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See detailThe effect of health related quality of life on reported use of health care resources in patients with osteoarthritis and rheumatoid arthritis: a longitudinal analysis.
Ethgen, Olivier ULg; Kahler, Kristijan H; Kong, Sheldon X et al

in Journal of Rheumatology (2002), 29(6), 1147-55

OBJECTIVE: In today's cost conscious environment, health services researchers are consistently trying to find ways to predict future health care resource utilization (HCRU) and its associated costs. We ... [more ▼]

OBJECTIVE: In today's cost conscious environment, health services researchers are consistently trying to find ways to predict future health care resource utilization (HCRU) and its associated costs. We evaluated the impact of health related quality of life (HRQL) on future HCRU in patients with arthritis. METHODS: A total of 642 patients with rheumatoid arthritis (RA) and 395 patients with osteoarthritis (OA) completed at least 2 and as many as 6 consecutive surveys at 6 mo intervals. Information collected included demographics, HRQL questionnaires [Medical Outcome Study Short Form 36 (SF-36), Western Ontario McMaster Universities Osteoarthritis Index (WOMAC), and the Stanford Health Assessment Questionnaire (HAQ)], and HCRU over the previous 6 months. Longitudinal data analysis was perfomed to assess the effect of HRQL on future HCRU. RESULTS: Statistically significant associations between HCRU and HRQL variables were noted. Higher rates of HCRU were found in those in the worst quarter compared with those in the best quarter of HRQL. With the HAQ, OA and RA patients in the worst quarter reported a 199% (p < 0.05) and 48% (p < 0.05) increase in rheumatologist visits, respectively. With the WOMAC Function, increases were as high as 196% (p < 0.05) in rheumatologist visits for patients with OA. Patients with RA with a high level of HRQL as measured by the SF-36 (physical component score) reported a decrease of 31% (p < 0.01) in general practitioner visits and a decrease of 52% (p < 0.01) in hospitalization (mental component score). CONCLUSION: These findings suggest that HRQL may be used to predict future health care consumption. Such an approach may lead to a more efficient allocation of resources by providing useful information to health care providers and health care decision makers. [less ▲]

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See detailEffect of heart failure on dipeptidyl peptidase IV activity in plasma of dogs.
Gomez, N.; Matheeussen, V.; Damoiseaux, Cécile ULg et al

in Journal of Veterinary Internal Medicine (2012)

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See detailThe effect of heat stress on freeze-drying and conservation of Lactococcus
Ziadi, M.; Touhami, Y.; Achour, M. et al

in Biochemical Engineering Journal (2005), 24(2), 141-145

The production of lactic acid bacteria isolated from Tunisian fermented milk (Lben): Lactococcus lactis subsp. lactis var. diacetylactis (SLT6) was investigated in fed-batch process. The final biomass ... [more ▼]

The production of lactic acid bacteria isolated from Tunisian fermented milk (Lben): Lactococcus lactis subsp. lactis var. diacetylactis (SLT6) was investigated in fed-batch process. The final biomass production was 2.2710(11) cfu/ml. The effect of heat stress, after fermentation, on the survival rate after freeze-drying and during storage was studied. The survival rate after freeze-drying of SLT6 subject of a thermal treatment was 38.8% whereas it was only 21.9% for unprocessed SLT6. The accelerated shelf life testing (ASLT) method was used to study the effect of heat stress on conservation of Lactococcus. The bacterium was stored at four different temperatures of 4, 15, 25 and 37 degrees C. Lactococcus cell kinetic behaviour suggested a first order reaction, and the effect of storage temperature was quantified by determination of the half-life period and the activation energy. The beneficial effect of heat stress is clearly associated with a half-life period of 86 days for SLT6T and only 48 days four SLT6NT stored at 4 degrees C. (c) 2005 Elsevier B.V. All rights reserved. [less ▲]

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See detailEffect of heat treatment of rennet skim milk induced coagulation on the rheological properties and molecular structure determined by synchronous fluorescence spectroscopy and turbiscan
Blecker, Christophe ULg; Habib-Jiwan, Jean-Michel; Karoui, Ramdhane

in Food Chemistry (2012), 135(3), 1809-1817

Heat treatment applied to milk induces denaturation of whey proteins, leading to a complex mixture of whey protein and whey protein coated casein micelles. The present paper investigates the effects of ... [more ▼]

Heat treatment applied to milk induces denaturation of whey proteins, leading to a complex mixture of whey protein and whey protein coated casein micelles. The present paper investigates the effects of heat treatment (60 and 80 °C during 20 min) and rennet-induced coagulation temperature (30 and 40 °C) determined by rheology, synchronous fluorescence spectroscopy (SFS) and turbiscan measurements. The gelation times determined by rheology and SFS increased with the increase of heat treatment applied to milk. The rise in temperature induced a decrease in the maximum curd firming rate and an increase in the viscosity of the investigated milk samples. The principal component analysis (PCA) applied, sepa- rately, to the SF and turbiscan spectra showed a clear discrimination between: (i) raw milks and heated milks; and (ii) milks renneted at 30 °C from those renneted at 40 °C. The results showed the ability of SFS as a rapid and non-destructive technique for the: (i) monitoring network structure and molecular inter- action during the coagulation process; and (ii) determination of gelation time of rennet-induced coagu- lation of studied milk samples. [less ▲]

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See detailEffect of hemicellulase preparations on performance parameters, intestinal viscosity and apparent nutrient digestibility of male broilers fed a wheat-based diet
Vandeplas, Sabrina ULg; Beckers, Yves ULg; Thewis, André ULg

Poster (2003)

Three experiments were conducted to investigate the mode of action of 5 hemicellulases supplemented to wheat-based broiler diets. Performances parameters and digesta viscosity were measured during growth ... [more ▼]

Three experiments were conducted to investigate the mode of action of 5 hemicellulases supplemented to wheat-based broiler diets. Performances parameters and digesta viscosity were measured during growth period. Total collection of excreta was carried out for determination of AMEn content of the diets and of apparent crude fat, Kjeldahl-nitrogen and crude cellulose digestibilities. Addition of xylanase improve daily body gain by 1 to 6% and feed conversion ration by 1,5 to 4%, as the apparent nutrient digestibility and the content of AMEn in the diets supplemented with enzymes. Although viscosity values seemed not correled to improved performances, Grindazym G shows an increase in the digesta viscosity for all the period of measurement. It can be concluded that the beneficial action of xylanases is more due to the improved release of the nutrients entrapped by the NSP, following degradation of these structures, than to the generally accepted decrease in digesta viscosity. [less ▲]

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See detailEffect of hemodiafiltration on pulmonary hemodynamics in a model of porcin endotoxin shock
Moonen, Marie ULg; LAMBERMONT, Bernard ULg; Kolh, Philippe ULg et al

in Journal of the American Society of Nephrology [=JASN] (2001), 12

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See detailEffect of hemodiafiltration on pulmonary hemodynamics in endotoxic shock
Lambermont, Bernard ULg; Kolh, Philippe ULg; Ghuysen, Alexandre ULg et al

in Artificial Organs (2003), 27(12), 1128-1133

Hemofiltration can improve pulmonary hemodynamics during septic shock. The main objective of the study was to determine whether hemodiafiltration (HDF) would also have beneficial effects on pulmonary ... [more ▼]

Hemofiltration can improve pulmonary hemodynamics during septic shock. The main objective of the study was to determine whether hemodiafiltration (HDF) would also have beneficial effects on pulmonary hemodynamics during septic shock. In the Endo group, six anesthetized pigs received a 0.5 mg/kg endotoxin infusion over 30 min. In the HDF group (n = 6), HDF was started 30 min after the end of the endotoxin infusion, while in the Control group (n = 4) they received HDF but no endotoxin infusion. Pulmonary hemodynamics were analyzed in detail with a four-element windkessel model. Although in the Control group, HDF did not alter pulmonary hemodynamic parameters, in the HDF group, it was responsible for an amplification of the deleterious pulmonary vascular response to endotoxin insult. Our results show that HDF must be used cautiously in septic shock since it can precipitate right heart failure by increasing pulmonary vascular resistance. [less ▲]

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See detailThe effect of heterogeneity of diffusion parameters on chloride transport in low-permeability argillites
Huysmans, Marijke; Dassargues, Alain ULg

in Environmental Earth Sciences (2013), 68(7), 1835-1848

Understanding flow and transport in low-permeability media is very important in the context of nuclear waste disposal, oil and gas reservoirs and long term evolution of groundwater systems. In low ... [more ▼]

Understanding flow and transport in low-permeability media is very important in the context of nuclear waste disposal, oil and gas reservoirs and long term evolution of groundwater systems. In low-permeability media, transport by diffusion is often the most important mass transport process. This study investigates the effect of the heterogeneity of diffusion parameters on mass transport in low-permeability media. A geostatistical approach for integrating heterogeneity of diffusion parameters in groundwater flow and transport models is proposed and applied to the Toarcian argillites in France which are studied in the framework of feasibility of storing radioactive waste in deep clayey massifs. Stochastic fields of the diffusion parameters of the Toarcian argillites (France) are generated based on 64 measured values of diffusion coefficient and diffusion accessible porosity and used as input for a 3D local-scale groundwater flow and transport model. The chloride concentrations computed by these heterogeneous models are compared to the measured chloride concentrations and to concentrations calculated with a model in which the Toarcian argillites are subdivided into several homogeneous zones. The heterogeneous simulations result in a slightly better correspondence between measured and calculated values and have the additional advantage that the measured diffusion coefficient values in the Toarcian are perfectly honored in the model. This study shows that small-scale variability of diffusion parameters has a significant effect on solute concentrations and omitting this heterogeneity may be a problem in transport calculations in low-permeability media, depending on the specific setting and objectives of the study. [less ▲]

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See detailThe effect of heterogeneity on the agreement between analytical and numerical solutions describing the recession flow in unconfined hillslope aquifers
Rocha, David; Feyen, Jan; Dassargues, Alain ULg

in Proceedings of the Int. Congress on Development, Environment and Natural Resources: Multi-level and multi-scale Sustainability (2007, July)

Analytical approximations or linearized versions of the Boussinesq equation, describing recession flow in unconfined hillslope aquifers have been applied extensively in the past. One of the major ... [more ▼]

Analytical approximations or linearized versions of the Boussinesq equation, describing recession flow in unconfined hillslope aquifers have been applied extensively in the past. One of the major assumptions in the conceptual formulation is isotropic homogeneity of the aquifer. Notwithstanding the effect of aquifer heterogeneity on recession flow has been pointed out [1], only recent studies are increasingly focusing on the quantification of this effect [2,3]. In the present research, the relevance of heterogeneity is being studied by analyzing the agreement between analytical approximations and the numerical solution of the receding flow in a layered unconfined aquifer with different domain configurations (horizontal, inclined and concave impervious bedrock). For the application of the analytical approximations use is made of the equivalent hydraulic conductivity concept. [less ▲]

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See detailThe effect of high CO2 during the first half of incubation on embryonic development after storage
Witters, A.; Debonne, M.; Everaert, Nadia ULg et al

Poster (2007)

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See detailThe effect of high CO2 during the second half of incubation on the acid-base balance regulation in the chicken embryo
Everaert, Nadia ULg; Linser, P.; Decuypere, E. et al

in Avian and Poultry Biology Reviews (2007)

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See detailEffect of High Doses of Oral Risedronate (20 Mg/Day) on Serum Parathyroid Hormone Levels and Urinary Collagen Cross-Link Excretion in Postmenopausal Women with Spinal Osteoporosis
Zegels, Brigitte ULg; Eastell, R.; Russell, R. G. et al

in BONE (2001), 28(1), 108-12

The present study describes the biological effects of risedronate, a pyridinyl bisphosphonate, on bone and assesses the safety and tolerability of risedronate when given at high doses, with or without ... [more ▼]

The present study describes the biological effects of risedronate, a pyridinyl bisphosphonate, on bone and assesses the safety and tolerability of risedronate when given at high doses, with or without calcium, to postmenopausal women with spinal osteoporosis. This single-center descriptive, double-blind, placebo-controlled, randomized, parallel group study included 32 postmenopausal white women with at least one radiographically confirmed vertebral compression fracture. Patients were randomized to one of four different dose regimen groups: (i) R-P, risedronate 20 mg/day for 14 days, followed by placebo for 42 days; (ii) R-CP-P, risedronate 20 mg/day for 14 days, followed by elemental calcium 1000 mg/day and placebo for 14 days, then by placebo for 28 days; (iii) R-CP-R-CP, risedronate 20 mg/day for 7 days, followed by elemental calcium 1000 mg/day and placebo for 21 days, then risedronate 20 mg/day for 7 days, and finally elemental calcium 1000 mg/day and placebo for 21 days; and (iv) P, placebo for 56 days. The biological response was investigated by measuring serum calcium, parathyroid hormone (PTH), and 2 h urinary pyridinoline/creatinine (Pyr/Cr) and deoxypyridinoline/creatinine (DPyr/Cr) ratios at baseline and at days 3, 7, 14, 21, 28, 35, 42, 49, 56, and 84. Overall, there were no consistent trends observed between the active group and placebo for serum calcium. In groups R-P, R-CP-P, and R-CP-R-CP, mean serum PTH levels were elevated above baseline values for the entire 56 day treatment period and remained elevated, although to a lesser extent, at the day 84 follow-up visit. The effect of calcium supplementation on PTH was variable. Urinary Pyr/Cr and DPyr/Cr ratios were decreased from baseline over the entire study period in all groups receiving risedronate. The maximum observed percent decreases from baseline for Pyr/Cr and DPyr/Cr were -46.9% and -58.8%, respectively, at day 49 in the R-CP-R-CP group. In conclusion, risedronate given orally at a dose of 20 mg/day, continuously for 7 or 14 days, resulted in the expected biological response in osteoporotic women. The time course of changes in PTH levels following cessation of dosing was unaffected by calcium supplementation. There was no evidence of a PTH-mediated rebound in bone resorption following cessation of therapy. Furthermore, based on collagen cross-link data, patients did not show an excessive reduction in bone turnover. [less ▲]

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See detailEffect of high osmolarity acclimation on tolerance to hyperosmotic shocks in L929 cultured cells.
Gilles, Raymond ULg; Belkhir, M.; Compère, Philippe ULg et al

in Tissue & Cell (1995), 27(6), 679-687

Application of abrupt, large hyperosmotic shocks induces in L929 cultured cells changes similar to those previously described in other cell types, notably a hypercondensation of the nuclear chromatin ... [more ▼]

Application of abrupt, large hyperosmotic shocks induces in L929 cultured cells changes similar to those previously described in other cell types, notably a hypercondensation of the nuclear chromatin. This paper shows that; 1) this phenomenon is concomitant with a complete disappearance of deoxyribonucleic acid, as visualized by immunogold labelling, from the nucleoplasmic spaces; 2) acclimation to high osmolarities (600 mOsm) by addition to the culture medium of NaCl, sorbitol or proline protects the cells from these effects, which appear to be largely attenuated-acclimated cells also survive much better to the osmotic shock than do control cells and; 3) the best protection seems to be provided by sorbitol and NaCl. Proline acclimation is less effective. These effects are discussed in terms of increased tolerance to NaCl load induced at the level of different macromolecules by so-called 'compensatory' organic compounds. [less ▲]

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