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See detailDetermination of the stability constants of uranyl/polymer complexes by differential pulse polarography
Leroy, D.; Martinot, Lucien; Jérôme, Christine ULg et al

in Polymer (2001), 42(10), 4589-4596

Differential pulse polarography (DPP) allows for the determination of the stability constants (K) and the stoichiometry (n) for the complexation of the UO22+ ion by various monomers and the parent ... [more ▼]

Differential pulse polarography (DPP) allows for the determination of the stability constants (K) and the stoichiometry (n) for the complexation of the UO22+ ion by various monomers and the parent polymers. The experimental observation is the shift of the totally reversible reduction wave UO22++e- UO2+ toward more cathodic potentials when the complexing monomer or polymer is added to an uranyl nitrate aqueous solution. This shift is, however, much greater when the polymer is used rather than the monomer, in line with a higher stability of the complexes (Kpolymer>>Kmonomer). A theoretical stability scale of the polymers/UO22+ complexes was compared to an experimental one based on dynamic leaching tests. [less ▲]

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See detailDetermination Of The Taxonomic Position And Characterization Of Yam Mosaic Virus Isolates Based On Sequence Data Of The 5'-Terminal Part Of The Coat Protein Cistron
Duterme, Olivier; Colinet, Dominique; Kummert, Jean et al

in Archives of Virology (1996), 141(6), 1067-1075

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See detailDetermination Of The Topology Of The Hydrophobic Segment Of Mammalian Diacylglycerol Kinase Epsilon In A Cell Membrane And Its Relationship To Predictions From Modeling
Decaffmeyer, Marc ULg; Shulga, Yv.; Dicu, Ao. et al

in Journal of Molecular Biology (2008), 383(4), 797-809

The epsilon isoform of diacylglycerol kinase (DGKepsilon) is unique among mammalian DGKs in having a segment of hydrophobic amino acids comprising approximately residues 20 to 41. Several algorithms ... [more ▼]

The epsilon isoform of diacylglycerol kinase (DGKepsilon) is unique among mammalian DGKs in having a segment of hydrophobic amino acids comprising approximately residues 20 to 41. Several algorithms predict this segment to be a transmembrane (TM) helix. Using PepLook, we have performed an in silico analysis of the conformational preference of the segment in a hydrophobic environment comprising residues 18 to 42 of DGKepsilon. We find that there are two distinct groups of stable conformations, one corresponding to a straight helix that would traverse the membrane and the second corresponding to a bent helix that would enter and leave the same side of the membrane. Furthermore, the calculations predict that substituting the Pro32 residue in the hydrophobic segment with an Ala will cause the hydrophobic segment to favor a TM orientation. We have expressed the P32A mutant of DGKepsilon, with a FLAG tag (an N-terminal 3xFLAG epitope tag) at the amino terminus, in COS-7 cells. We find that this mutation causes a large reduction in both k(cat) and K(m) while maintaining k(cat)/K(m) constant. Specificity of the P32A mutant for substrates with polyunsaturated acyl chains is retained. The P32A mutant also has higher affinity for membranes since it is more difficult to extract from the membrane with high salt concentration or high pH compared with the wild-type DGKepsilon. We also evaluated the topology of the proteins with confocal immunofluorescence microscopy using NIH 3T3 cells. We find that the FLAG tag at the amino terminus of the wild-type enzyme is not reactive with antibodies unless the cell membrane is permeabilized with detergent. We also demonstrate that at least a fraction of the wild-type DGKepsilon is present in the plasma membrane and that comparable amounts of the wild-type and P32A mutant proteins are in the plasma membrane fraction. This indicates that in these cells the hydrophobic segment of the wild-type DGKepsilon is not TM but takes up a bent conformation. In contrast, the FLAG tag at the amino terminus of the P32A mutant is exposed to antibody both before and after membrane permeabilization. This modeling approach thus provides an explanation, not provided by simple predictive algorithms, for the observed topology of this protein in cell membranes. The work also demonstrates that the wild-type DGKepsilon is a monotopic protein. [less ▲]

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See detailDetermination of the tortuosity of porous materials from binary or grey-tone tomographic reconstructions
Gommes, Cédric ULg; Almazan, Maria del Carmen; Léonard, Angélique ULg et al

Conference (2008, November)

Detailed reference viewed: 47 (8 ULg)
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See detailDetermination of the total glucosinolate content in rapeseed seeds by liquid chromatography: comparative study between the rapid isocratic and the reference gradient methods by a ring test
Quinsac, A.; Krouti, M.; Ribaillier, D. et al

in Ocl-Oleagineux Corps Gras Lipides (1998), 5(5), 398-406

Isocratic liquid chromatography (LC) on cyanopropyl bonded silica column is an alternative method to gradient IC on octadecyl bonded silica column involved in the standardized method for total ... [more ▼]

Isocratic liquid chromatography (LC) on cyanopropyl bonded silica column is an alternative method to gradient IC on octadecyl bonded silica column involved in the standardized method for total glucosinolotes (GSL) analysis in rapeseed seeds. Its advantages consist in more simple apparatus and eluent, and a shorter analysis duration. In the aim of a next standardization of isocratic LC, the performances of both methods ore compared on three rapeseed samples during a ring-test including six laboratories. Statistical treatment or the results according to ISO 5725 shows, with a risk level of 5%, the equivalency of both methods to determine total GSL content in rapeseed seeds. The elution mode appears to have no significant effect on results in opposite to the internal standard when its purity is not well-known. [less ▲]

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See detailDetermination of the Total Ionospheric Electron Content with GPS - Difficulties and their Solution
Wanninger, Lambert; Sardon, E.; Warnant, René ULg

in Kersley, Len (Ed.) Proceedings of Beacon Satellite Symposium '94 (1994)

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See detailDetermination of the true ileal digestibility of amino acids in pigs by means of 15N-labelled diets or animals.
Leterme, Pascal; Thewis, André ULg; Van leeuwen, Piet et al

Conference (1993, August)

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See detailDetermination of the true ileal digestibility of amino acids in pigs by means of 15N-labelled diets or animals.
Leterme, Pascal; Van Leeuwen, Piet; Thewis, André ULg et al

in Proceeding of the 6th International Symposium on Digestive Physiology in Pigs, n°80. (1994)

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See detailDetermination of the true ileal digestibility of amino acids in pigs by means of 15N-labelled diets. Preliminary results.
Leterme, Pascal; Thewis, André ULg; Genot, L. et al

in Nitrogen flow in pig production and environmental consequences: Proceedings of the First International Symposium. (1993)

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See detailDetermination of Thiamin and Its Phosphate Esters in Cultured Neurons and Astrocytes Using an Ion-Pair Reversed-Phase High-Performance Liquid Chromatographic Method
Bettendorff, Lucien ULg; Peeters, Maryline; Jouan, Caroline ULg et al

in Analytical Biochemistry (1991), 198(1), 52-59

A sensitive method, based on fluorescence detection, for the determination of thiamin derivatives after precolumn derivatization is described. The separation is achieved on a PRP-1 column using ion-pair ... [more ▼]

A sensitive method, based on fluorescence detection, for the determination of thiamin derivatives after precolumn derivatization is described. The separation is achieved on a PRP-1 column using ion-pair reversed-phase HPLC. This method is especially well adapted to the detection of thiamin triphosphate in complex mixtures such as tissue extracts. The detection limit for TTP is 50 fmol. The contents of thiamin derivatives were determined in primary cultures of rat cerebellar granule neurons and cerebral astrocytes. The amount of TTP is about five times higher in neurons than in astrocytes. Thus in rat brain TTP seems to be essentially associated with neurons and the intracellular concentration is estimated to be about 0.2 microM. Our results suggest the existence, in nerve cells, of specific regulatory mechanisms not related to the blood-brain barrier and responsible for the maintenance of thiamin homeostasis in brain. [less ▲]

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See detailDetermination of thiamine and its phosphate esters by reversed-phase HPLC at femtomole levels. Application to bioelectrogenesis.
Bettendorff, Lucien ULg; Schoffeniels, Ernest; Schmartz, Denis et al

in Changeux, Jean-Pierre; Maelicke, A.; Neumann, E. (Eds.) Molecular Basis of Nerve Activity (1985)

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See detailDetermination of thiamine and its phosphate esters in human blood serum at femtomole levels
Bettendorff, Lucien ULg; Grandfils, Christian ULg; De Rycker, Cécile et al

in Journal of Chromatography. B : Analytical Technologies in the Biomedical & Life Sciences (1986), 382

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See detailDetermination of thiamine and thiamine phosphates as thiochrome derivatives by reversed phase chromatography on polystyrene packing materials
Bontemps, José; Bettendorff, Lucien ULg; Lombet, Jacques et al

in Chromatographia (1984), 18

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See detailDetermination of thiamine and thiamine phosphates in excitable tissues as thiochrome derivatives by reversed phase high-performance liquid chromatography on octadecyl-silica.
Bontemps, José; Philippe, P.; Bettendorff, Lucien ULg et al

in Journal of Chromatography. B : Biomedical Applications (1984), 307

Detailed reference viewed: 41 (6 ULg)
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See detailDetermination of tissue-specific CK-MB2 in serum after myocardial damage
Hing, M.; Chapelle, Jean-Paul ULg

in European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies (1993), 31

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See detailDetermination of total arsenic in foods by HGAAS
Kabengera, Ch.; Bodart, P.; Hubert, Philippe ULg et al

Conference (2000)

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See detailDetermination of total glucosinolate in rapeseed (colza). Activities of BCR-programme Community Bureau of Reference .
Wathelet, Jean-Paul ULg; Severin, M.; Wagstaffe, P. J.

in GCIRC Bulletin (1990)

Detailed reference viewed: 25 (2 ULg)