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See detailDevelopment of formulated elicitors to control bioagressors of wheat: which interests?
Le Mire, Géraldine ULg; Deleu, Magali ULg; Ongena, Marc ULg et al

Poster (2014, February 07)

Crop plants are constantly exposed to a wide range of environmental stresses that can cause major productivity loss. Fungicide treatments are the most efficient disease control methods used up to now ... [more ▼]

Crop plants are constantly exposed to a wide range of environmental stresses that can cause major productivity loss. Fungicide treatments are the most efficient disease control methods used up to now. However, the environmental and health issues caused by these chemicals make it urgent to develop safer methods for pathogen control. In the optic of a sustainable agriculture, several biocontrol tools have been developed during the last decades. Elicitors are increasingly subject to research as interesting alternatives to phytosanitary products. Yet, few efficient formulations exist on the market, and no product has been designed specifically to protect wheat crops. This study will thus focus on the the manipulation of host natural defenses through the use of elicitors as a preventive treatment against major wheat diseases such as Septoria tritici, Fusarium graminearum and Fusarium culmorum. [less ▲]

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See detailDEVELOPMENT OF FROZEN-FRIED YAM SLICES: OPTIMIZATION OF THE PROCESSING CONDITIONS
Toure, Yetioman ULg; Nindjin, Charlemagne; Brostaux, Yves ULg et al

in African Journal of Food, Agriculture, Nutrition and Development (2012), 12(7), 7055-7071

The research performed on yam processing mainly concerns the production of crisps and flour. However, its transformation into deep-frozen French fries does not necessitate any other equipment than those ... [more ▼]

The research performed on yam processing mainly concerns the production of crisps and flour. However, its transformation into deep-frozen French fries does not necessitate any other equipment than those used for potatoes. The industrial process of production of frozen French fries traditionally includes a pre-frying step. These steps contribute to the development of color and crispness, and the oil partially absorbed inhibits dehydration during the freezing step. The aim of this study was to optimize frying conditions of deep-frozen fried yam (Dioscorea cayenensis var Kponan) slices. The effect of pre-frying time and temperature, final frying time and temperature on the oil uptake, texture, dry matter and colour of the fried yam slices has been studied. Frying conditions optimized with Box-Behnken experimental design were short pre-frying and frying conditions at high temperature characterized by pre-frying temperature at 157-170°C during 5-9s and frying temperature at 181-188°C for 2min 15s-2min 30s; or long pre-frying and frying conditions at low temperature characterized by pre-frying temperature at 150-158°C during 10-15s and frying temperature 170-177°C for 3-3min 15s. An adiabatic system was also developed by means of an insulator in which the core temperature of fried yam slices can be maintained constant at about 55°C after 15min of cooling, facilitating texture measurements at constant temperature. The present results may help in choosing the yam slices frying condition to be applied in order to achieve the desirable fried yam slices quality, required for protection against certain diseases like obesity. These models may also provide guidance as to how to control these quality parameters by altering four key environmental factors, pre-frying temperature and time and, final frying temperature and time. This process can also be commercialized and does not necessitate any other cost for equipment than those used for potatoes French fries and might be an interesting way of added value processing for this highly perishable yam tuber. [less ▲]

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See detailDevelopment of functional material for direct manufacturing for ceramic and metal parts by Optoform process
Clarinval, Anne-Marie; Carrus, Raoul; Vaneetveld, Grégory ULg et al

Conference (2004, September)

Optoform LLC developed a technology to process paste compositions based upon photo-curable resins and fillers. This new process is called by Optoform LLC : the direct composite manufacturing This ... [more ▼]

Optoform LLC developed a technology to process paste compositions based upon photo-curable resins and fillers. This new process is called by Optoform LLC : the direct composite manufacturing This technique is quite flexible and allows the development of very different materials(polymeric, metallic, ceramic). This paper is an overview of the use limitation and the advantages of materials developed by the CRIF or by other teams. Materials requiring or not a post-processing step have been developed. The limits or restrictions of use of these materials have been studied in order to meet the requirements and needs of various industrial sectors in the United States and in Europe (e.g. car racing, aerospace and aeronautical sectors, tooling industries, …). [less ▲]

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See detailDevelopment of functionalized nanoparticles for vaccine delivery to dendritic cells: a mechanistic approach
Silva, Joana M.; Vandermeulen, Gaëlle; Oliveira, Vanessa G. et al

in Nanomedicine (2014), 9(17), 2639-2656

Aim: Produce biodegradable nanoparticles to target antigen-presenting cells and evaluate their potential to be used as a vaccine delivery system. Materials & methods: Untargeted PEGylated PLGA-based ... [more ▼]

Aim: Produce biodegradable nanoparticles to target antigen-presenting cells and evaluate their potential to be used as a vaccine delivery system. Materials & methods: Untargeted PEGylated PLGA-based nanoparticles and mannose-grafted nanoparticles were formulated and physicochemically characterized. Immortalized and primary antigen-presenting cells were used to study nanoparticle internalization patterns. The endocytic pathways and intracellular trafficking followed by nanoparticles were also investigated. Results & discussion: Nanoparticles displayed mannose residues available for binding at the nanoparticle surface. Different nanoparticle internalization patterns by immortalized and primary antigen presenting cells were verified. Macropinocytosis, clathrin-mediated endocytosis, caveolin- and lipid raft-dependent endocytosis are involved in nanoparticles internalization. Nanoparticles demonstrate both endo-lysosomal and cytosolic localizations and a tendency to accumulate nearby the endoplasmic reticulum. Conclusion & future perspective: The developed nanoparticles might drive antigens to be presented through MHC class I and II molecules to both CD8+ and CD4+ T cells, favoring a complete and coordinated immune response. [less ▲]

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See detailDevelopment of gelling properties of inulin by microfluidization
Ronkart, Sebastien N; Paquot, Michel ULg; Deroanne, Claude et al

in Food Hydrocolloids (2010), 24(4), 318-324

In this paper, we report the impact of a microfluidic device (Microfluidizer (R)) on the development of gelling properties of inulin-water systems. Inulin dispersions at a concentration of 2, 7 and 15%, w ... [more ▼]

In this paper, we report the impact of a microfluidic device (Microfluidizer (R)) on the development of gelling properties of inulin-water systems. Inulin dispersions at a concentration of 2, 7 and 15%, w/w, were subjected to microfluidization treatments at 30 MPa with various numbers of circulations in the apparatus (1, 2 or 5 passes). The high shear stress treatment did not induce a chemical composition change of inulin. However, it allowed an increase of the gel-like behavior of the system as well as the viscosity of the inulin dispersion, transforming a visual aspect of the product similar to milk, to a system similar to yogurt or margarine depending on the concentration and the number of passes in the Microfluidizer (R). The viscosity increased with both the number of passes and the inulin concentration. Granulometry as well as optical and electronic microscopy ascertained the reduction of the particle size and the formation of a network composed of agglomerates which interacted with the solution and thus led to textural modifications. (C) 2009 Elsevier Ltd. All rights reserved. [less ▲]

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See detailDevelopment of GEQUAC as a new group contribution method for strongly non-ideal mixtures
Ehlker, Gerhard H; Pfennig, Andreas ULg

in Fluid Phase Equilibria (2002), 203((1-2)), 53-69

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See detailDevelopment of high input systems of cereal production in Europe
Falisse, André; Bodson, Bernard ULg

Scientific conference (1982)

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See detailDevelopment of Highly Active doped Titania Photocatalysts by Aqueous Sol-Gel Processing
Malengreaux, Charline ULg; Douven, Sigrid ULg; Poelman, Dirk et al

Poster (2012, November)

Detailed reference viewed: 29 (14 ULg)
See detailDevelopment of Highly Active doped Titania Photocatalysts by Aqueous Sol-Gel Processing
Malengreaux, Charline ULg; Douven, Sigrid ULg; Poelman, Dirk et al

Poster (2012, December)

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See detailDevelopment of HY1 hybrid embryos between a cultivar of Phaseolus vulgaris L. and a wild genotype of Phaseolus coccineus L.
Nguema Ndoutoumou, Pamphile; Toussaint, André ULg; Baudoin, Jean-Pierre ULg

in International Journal of Biological and Chemical Sciences (2009), 3(1), 20-26

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See detailDevelopment of Hyphenated Methods for the Comprehensive Analysis of Complex Biological Samples
Brasseur, Catherine ULg

Doctoral thesis (2015)

The successful analysis of complex mixtures requires the combination of highly efficient separation techniques with advanced detection technologies to provide individual structural information. The ... [more ▼]

The successful analysis of complex mixtures requires the combination of highly efficient separation techniques with advanced detection technologies to provide individual structural information. The coupling of gas chromatography and mass spectrometry was one of the first steps in hyphenated method development. Nowadays, due to continuous hardware improvement, the term hyphenation has been extended to multidimensional separations and/or multiple detectors. The objective of the thesis is to develop hyphenated methods to extend the range of applications and propose solutions to improve the characterization of specifically selected complex samples. The main hyphenated technique that was used is comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC×GC-TOFMS). Three thematic areas, presenting different levels and types of complexity were investigated: Forensic geotaphonomy with grave soil samples, lignocellulose biomass valorization with termite gut microbiota samples, and human monitoring for emerging organohalogen contaminants with blood samples. Data processing strategies were also developed in order to extract the relevant information from the large quantities of data produced. The different strategies included retention time alignment, comparative data processing, multivariate analysis using unsupervised learning algorithm such as principal component analysis (PCA), univariate analysis of variance (one-way ANOVA), and scripting for automated filtering of mass spectra. All developed methods allowed to successfully improve the characterization of samples originating from the different areas. For the first time, and because of the high level of specificity of GC×GC-TOFMS, the presence of methyl-branched alkane compounds was highlighted in grave soil samples, allowing to develop a screening method that has the capacity to differentiate between soils at proximity of buried decaying pig carcasses and control soils. This strategy could complement other approaches and contribute to aid forensic geotaphonomy investigators. In the biomass valorization area, more than 300 compounds were isolated and partly identified in the 1µL fluid volume available from the termite gut. A comparative study demonstrated that the adaptation of the termite system to non-optimal carbon sources is reflected in the metabolite profile. These results demonstrated the potential interest to investigate metabolite profiling with state-of-the-art separation science tools, to contribute to a better understanding of how termites efficiently degrades lignocellulose. In the human monitoring area, the analysis of serum samples using GC×GC-TOFMS and scripting allowed the efficient reduction of the data matrix from thousands of detected signals to a few important dozens, including the environmental contaminants of interest present in human blood plasma. Such an approach reduced data processing and reviewing time by several orders of magnitude while maintaining a high degree of identification power. This exploratory approach could result in the identification of emerging toxicants and help regulation bodies to gather better information before they rule on emerging issues. GC with high resolution mass spectrometry (HRMS) was also used, using a selective approach to evaluate human exposure to dechloranes, a family of emerging halogenated flame retardants recently reported as significantly present in the environment. A comprehensive approach was also developed using GC×GC coupled to high resolution TOFMS (HRTOFMS). The developed methodology will facilitate the monitoring of these emerging dechlorane contaminants and analogues in future exploratory studies. [less ▲]

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See detailDevelopment of in vitro techniques for the elimination of Cucumber Mosaic Virus from banana (Musa spp.).
Helliot, B.; Panis, B.; Hernandez, R. et al

in Banana improvement: cellular, molecular biology, and induced mutations.Proceedings of a meeting held in Leuven, Belgium, 24-28 September 2001 (2004)

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See detailDevelopment of indicators to quantify nitrogen rejections of grazing dairy cows according to fertilisation types
Meura, Stéphane; Lambert, Richard; Dufrasne, Isabelle ULg et al

in Permanent and temporary grassland plant, environment and economy; A. De Vliegher and L. Carlier (Eds); Proceedings of 14th symposium of the European Grassland Federation (2007)

Nitrogen (N) rejections are a problem of importance in cattle production. In pasture, the amounts of N rejected by the cattle are large since grass N content exceeds the ‘animals’ requirements. The ... [more ▼]

Nitrogen (N) rejections are a problem of importance in cattle production. In pasture, the amounts of N rejected by the cattle are large since grass N content exceeds the ‘animals’ requirements. The present study aims to quantify, with dairy cows in a rotational system, N rejections from urine. The trial paddocks were fertilized with compost, slurry or mineral N and grazed during 7 days by 35 dairy cows in late lactation. Milk urea concentration was determined in tank milk samples every day. Urine samples were taken from each cow at the 3rd and the 5th day after the entry in the grazing plot. Urine N and creatinin were determined to quantify urine N excretion. These observed values were compared to urine N excretion data obtained from dry matter intake and grass N content. The mean observed urine N excretion of 312 g N day-1 was similar to the mean calculated urine N excretion of 330 g N day-1. The correlation between calculated urinary N excretion and observed urinary N excretion was significant (P<0.001; r2= 22.4%) and the correlation between observed urinary N excretion and milk urea content tended to be significant (P<0.10; r2 =60%). From these data, it appears that urine N excretion prediction can be more precise with tank milk urea than with urine N excretion calculated from N intake. This research has to be continued and to be repeated during other grazing periods and with cows at different lactation periods to validate the results. [less ▲]

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See detailThe development of knowledge regarding the characteristics of the proteose peptone fraction of milk: Techno-functional and biological properties. A review
Karamoko, Gaoussou ULg; Anihouvi, Prudent; Blecker, Christophe ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment (2013), 17(2), 373-382

The total proteose-peptone fraction (TPP) is defined as a heat-stable soluble fraction of milk, representing about 10% of the whey protein. TPP is divided into two classes according to its origin. The ... [more ▼]

The total proteose-peptone fraction (TPP) is defined as a heat-stable soluble fraction of milk, representing about 10% of the whey protein. TPP is divided into two classes according to its origin. The first class consists of proteolysis fragments of the β-casein from the N-terminal region. These are non-hydrophobic fractions, which are the highly soluble β-CN-5P (f1-105/107); β-CN-4P (f1-28) and β-CN-1P (f29-105/107) respectively called PP5 (14.3 kDa), PP8S (9.9 kDa), and PP8F (4 kDa). The second class includes the hydrophobic fractions of glycoproteins, whose major constituents are a glycoprotein LP28, the highly hydrophobic glycoprotein LP18 and a hydrophobic peptide with apparent Mr, respectively 28 kDa, 18 kDa and 11 kDa. TPP has numerous interesting characteristics such as techno-functional properties (emulsifying and foaming actions) and biological properties (lipolysis inhibition and antimicrobial activities), making TPP usable as a potential functional ingredient for industry. In addition, these functional properties are partly governed by the major components including glycoproteins, such as LP28, due to their hydrophobic nature. [less ▲]

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See detailDevelopment of lead-210 measurement in peat using polonium extraction. A procedural comparison
De Vleeschouwer, François; Sikorski, Jarek; Fagel, Nathalie ULg

in Geochronometria (2010)

Two chemical treatments for lead-210 measurement were compared on the sub-surface samples of a core from an ombrotrophic bog from East Belgium. The classical procedure involves a concentrated acid ... [more ▼]

Two chemical treatments for lead-210 measurement were compared on the sub-surface samples of a core from an ombrotrophic bog from East Belgium. The classical procedure involves a concentrated acid extraction of polonium. However, this treatment represents substantial health risks together with unknowns regarding both the degree of cleanliness and the Po extraction rate, and most importantly, is rather time consuming. We developed here an improved procedure involving an ashing step prior to acid extraction. This allows substantial improvements such as: 1/ the use of a relatively small amount of acid compared to the classical procedure and 2/ the substantial reduction of a total sample digestion time. Measurements of 210Pb concentrations were conducted by alpha spectrometry. Results show a good agreement of unsupported 210Pb activity obtained for both procedures, although some unknowns remain concerning the adsorption of 210Po on the plastic test tube, the volatilization of a small amount of ash, or the absorption of alpha particle at the alpha source surface. This however should not affect the 210Pb measurement as all the samples are spiked prior to ashing (i.e. the recoveries are fully monitored). Through this study, we are suggesting researchers to follow this new procedure in order to increase safety, cleanliness, better recovery and substantial time gain. [less ▲]

Detailed reference viewed: 53 (0 ULg)