Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailDevelopment of an Evidence-Based Calibration Methodology Dedicated to Energy Audit of Office Buildings. Part 1: Methodology and Modeling.
Bertagnolio, Stéphane ULg; Andre, Philippe ULg

in Proceedings of the 10th REHVA World Congress - Clima 2010 (2010, May)

1 Introduction To promote improvements in the HVAC installations of existing buildings, the article 9 of the EPBD directive establishes mandatory audits and inspections of air-conditioning systems. The ... [more ▼]

1 Introduction To promote improvements in the HVAC installations of existing buildings, the article 9 of the EPBD directive establishes mandatory audits and inspections of air-conditioning systems. The development of auditing tools and procedures and the training of future auditors are the main objectives of the HARMONAC project launched in 2007. Four audit stages are generally distinguished: benchmarking, inspection, detailed audit and investment grade audit. Answering the questions encountered along the audit process requires some diagnosis, which has to be established on the basis of the very scarce information currently available: technical data contained in as-built files actually available and, generally, very global recordings of energy consumptions. A dynamic hourly building energy simulation (BES) model is being developed in the frame of the HARMONAC project to help the auditor in establishing his diagnosis despite of the lack of data. Two simulation tools dedicated to assist the auditor are developed on the basis of this equation-based BES model: - The first tool is dedicated to a preliminary benchmarking of the installation. - The second tool is used from inspection stage to evaluation of selected energy conservation measures (ECMs). 2 Simulation-based audit methodology The first step of the audit methodology consists in gathering the as-built and utility data, applying a first treatment and analysis method to this data and using the BES model to perform benchmarking. For this benchmarking targeted work, the actual building is coupled to a typical HVAC system whose performances are defined according to EPBD standards. If the benchmarking indicates that potential energy savings should be studied in the building, the calibration-based audit procedure is launched. In the second step, the BES model is integrated in an iterative evidence-based calibration method adapted to audit purposes. The calibration starts directly at the beginning of the inspection, before realizing any additional measurement. This calibration process includes sensitivity and measurement issues, in order to help the auditor identifying critical points and understanding the building operation. Graphical and statistical methods are used in order to quantify and evaluate if the calibrated baseline model satisfies the predefined tolerances. Finally, the calibrated baseline model can be used to evaluate ECMs and savings uncertainty. The development and the implementation of the BES model are presented in this paper. The mains issues of the proposed calibration-based audit methodology are discussed. An example of application of the whole methodology (from benchmarking to evaluation of ECMs) is presented in a companion paper. [less ▲]

Detailed reference viewed: 315 (21 ULg)
Full Text
Peer Reviewed
See detailDevelopment of an Evidence-Based Calibration Methodology Dedicated to Energy Audit of Office Buildings. Part 2: Application to a typical office building in Belgium
Andre, Philippe ULg; Fabry, Bertrand ULg; Bertagnolio, Stéphane ULg et al

in Proceedings of the 10th REHVA World Congress - Clima 2010 (2010, May)

The development of specific tools for the audit of air-conditioning systems is described in a companion paper. These developments are carried out in the frame of the HarmonAC project launched in 2007 in ... [more ▼]

The development of specific tools for the audit of air-conditioning systems is described in a companion paper. These developments are carried out in the frame of the HarmonAC project launched in 2007 in order to assist the implementation of the EPBD, article 9. The development of these tools was continously supported by the application to case studies. This paper reports on the application of the tools within an audit procedure applied to a typical office building in Belgium, which is in operation for more than 20 years. The first step of the work consisted in analyzing the current performance of the building which proved to point out a number of problems in the operation of the building. As it is not rarely the case for running office buildings, these problems were due to a lack of commissioning and a progressive decrease of the performances due to a poor management. Application of the first stage “Benchmark” tool to this building led to a very high discrepancy between the measured consumptions and the calculated ones. This appeared mainly for the electricity consumption but also for the gas consumption. Calibration of the simulation tool, mainly by representing more exactly the management of the building and the real internal gains improved the accuracy of the calculations to a large extent. After calibration of the building and HVAC model, the application of a second tool (SimAudit) allowed to evaluate the savings obtained by the implementation of a number of Energy Conservation Opportunities (ECOs). In this case, the dominant ECOs were in relation with the operation of the building: change of schedules, modification of setpoints; implementation of free-cooling strategies; provision of heat recovery and move to reversibility of the chillers. Reasonable estimates of the savings can be obtained following the calibration/simulation process. The paper will highlight some of the most relevant ECOs and provide an order of magnitude for the savings in this kind of building and in a mild climate. [less ▲]

Detailed reference viewed: 87 (16 ULg)
Peer Reviewed
See detailDevelopment of an ex-vitro system allowing plant-bacteria interactions through VOCs in the context of water stress
Mendaluk, Magdalena ULg; Baudson, Caroline ULg; Delory, Benjamin ULg et al

Poster (2014, February 07)

Water stress is one of the major environmental factors limiting the crop productivity . Plant stress responses are very complex and drought tolerance may be linked to the presence of specific ... [more ▼]

Water stress is one of the major environmental factors limiting the crop productivity . Plant stress responses are very complex and drought tolerance may be linked to the presence of specific microorganisms in the rhizosphere. Indeed, some plant growth promoting bacteria (PGPR) strains have been found to improve plant growth under abiotic stresses. Among the many mechanisms by which those PGPR can support plant growth, the emission of volatile organic compounds (VOCs) and their biological impact are still under study. The aim of this work is to evaluate the interaction between the model grass Brachypodium distachyon (Bd21) and two strains of PGPR. The impact of volatile emission on Bd21 growth was studied using an ex-vitro cocultivation system without physical contact between plant and bacteria during 10 days. This peculiar system was developed to assess bacterial VOCs impacts on plants under realistic growth and stress conditions. In parallel, the response of Bd21 seedlings to water deficit induced by polyethylene glycol 6000 (PEG 6000) was studied to establish contrasted growth conditions regarding water availability [less ▲]

Detailed reference viewed: 59 (3 ULg)
Full Text
Peer Reviewed
See detailDevelopment of an experimental device allowing plant-plant interaction studies and in situ dynamic trapping of volatile organic compounds emitted by barley (Hordeum distichon L.) roots
Delory, Benjamin ULg; Delaplace, Pierre ULg; Fauconnier, Marie-Laure ULg et al

in Communications in Agricultural and Applied Biological Sciences (2013, February 08), 78(1), 97-102

In response to wounding or herbivore attack, leaves and roots of higher plants release volatile organic compounds (VOCs). To date, VOCs analysis and plant−plant interaction studies have been mainly ... [more ▼]

In response to wounding or herbivore attack, leaves and roots of higher plants release volatile organic compounds (VOCs). To date, VOCs analysis and plant−plant interaction studies have been mainly performed on aboveground plant tissues, leaving the roles played by root VOCs in plant−plant interaction unexplored. In this context, this project aims at setting up an original experimental device allowing both dynamic trapping of VOCs emitted by mechanically damaged H. distichon roots and the study of the roles played by root VOCs in intra and interspecific plant−plant interactions. The experimental device consists of Barley seedlings cultivated in closed PTFE reactors filled with wet sand. Before being analysed by gas chromatography−mass spectrometry, root VOCs are trapped via a dynamic system on Tenax cartridges using a charcoal-filtered and humidified air. Preliminary results show that 7 day-old wounded Barley roots emit C9 fatty acid derivatives (E-non-2-enal and nona-2,6-dienal) as major compounds, contrasting with aboveground plant tissues that mainly emit C6 alcohols, aldehydes, and their derivative esters. For plant−plant interaction studies, receiver plants are exposed to an airflow enriched with VOCs from root damaged Barley plants of the same age. [less ▲]

Detailed reference viewed: 167 (33 ULg)
Full Text
Peer Reviewed
See detailDevelopment of an experimental equipment for the identification of constitutive laws
Flores, Paulo; Rondia, Etienne ULg; Habraken, Anne ULg

in International Journal of Forming Processes (2005), (Special Issue), 117-137

An experimental testing equipment for sheet samples is built in order to identify material parameters ofcomplex phenomenological constitutive laws. This equipment consists in a bi-axial test machine able ... [more ▼]

An experimental testing equipment for sheet samples is built in order to identify material parameters ofcomplex phenomenological constitutive laws. This equipment consists in a bi-axial test machine able to perform plane strain and simple shear tests separately or simultaneously and a Miyauchi simple shear test device. An optical extensometer is used to identify the strain field. The article is focused on the validation of the results of this new equipment by comparing with results obtained by finite elements (FE) simulations of already identified materials. The results are satisfactory and the bases for further material identification are established. [less ▲]

Detailed reference viewed: 84 (23 ULg)
Full Text
Peer Reviewed
See detailDevelopment of An Expert System for the Identification of Nonlinear Vibrating Systems
Dimitriadis, Grigorios ULg; Vio, Gareth Arthur; Shi, Dongfeng

in Brennan, M. J.; Liguore, S.; Mace, B. R. (Eds.) et al Proceedings of the IX International Conference on Recent Advances in Structural Dynamics (2006, July)

The aim of the present work is to attempt to create a logical framework to be used for the identification of nonlinear systems. It is assumed that no single identification method is general enough to work ... [more ▼]

The aim of the present work is to attempt to create a logical framework to be used for the identification of nonlinear systems. It is assumed that no single identification method is general enough to work with a significant range of systems. Therefore, this framework is based on the development of an Expert System that will detect and quantify the nonlinearity present in a given dynamical system and, subsequently, determine and apply the most suitable nonlinear system identification method or methods. [less ▲]

Detailed reference viewed: 20 (8 ULg)
Full Text
Peer Reviewed
See detailDevelopment of an extended Drucker-Prager hardening model for cross-anisotropy of soft rocks
François, Bertrand; Collin, Frédéric ULg; Dizier, Arnaud et al

in Pietruszczak, S.; Pande, G.N. (Eds.) PROCEEDINGS OF THE 2 nd INTERNATIONAL SYMPOSIUM ON COMPUTATIONAL GEOMECHANICS (COMGEO II) (2011)

Detailed reference viewed: 52 (9 ULg)
Full Text
See detailDevelopment of an FDS-SAFIR interface
Tondini, Nicola; Franssen, Jean-Marc ULg

Scientific conference (2011, November 15)

A weak coupling strategy from CFD models to F.E. models is being developed on the base of an interface file. The software FDS and SAFIR are used in the applications.

Detailed reference viewed: 76 (6 ULg)
Full Text
See detailDevelopment of an immobilisation device for treatment of patients after breast conserving surgery for mammary carcinoma.
COUCKE, Philippe ULg; WONNER, Michel ULg; JANVARY, Zsolt Levente ULg et al

Poster (2010, September)

To develop an immobilisation device for prone positioning for breast irradiation. The basic requirements for the device are: rapid and accurate patient (re)positioning, compatibility with modern imaging ... [more ▼]

To develop an immobilisation device for prone positioning for breast irradiation. The basic requirements for the device are: rapid and accurate patient (re)positioning, compatibility with modern imaging devices and easy to handle by the treating technologists. [less ▲]

Detailed reference viewed: 33 (2 ULg)
Peer Reviewed
See detailDevelopment of an implantable integrated MEMS pressure sensor using polyimide epoxy composite and Atomic Layer Deposition for encapsulation
Gembaczka, P; Görtz, M; Kraft, Michael ULg

in Proceedings of "48th DGBMT Annual Conf. : Hannover, Germany Octobre 2014" (2014, October)

Detailed reference viewed: 11 (0 ULg)
Full Text
Peer Reviewed
See detailDevelopment of an in vitro culture method for heart-shaped embryo in Phaseolus vulgaris.
Mergeai, Guy ULg; Baudoin, Jean-Pierre ULg

in Annual report of the Bean Improvement Cooperative (1990), 33

Detailed reference viewed: 5 (2 ULg)
Full Text
Peer Reviewed
See detailDevelopment of an in vitro culture method of very immature embryos to assist wide interspecific hybridisation in Phaseolus.
Geerts, P.; Baudoin, Jean-Pierre ULg; Mergeai, Guy ULg

in 3rd European conference on grain legumes : Opportunities for high quality, healthy and added-value crops to meet European demands, 14-19 November 1998, Valladolid, Spain (1998)

Detailed reference viewed: 11 (6 ULg)
Full Text
Peer Reviewed
See detailDevelopment of an in vitro culture techniques adapted to Phaseolus polyanthus and P. vulgaris embryos.
Geerts, P.; Baudoin, Jean-Pierre ULg; Mergeai, Guy ULg

in Bean Improvement Cooperative (1998), 41

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailDevelopment of an in vitro endotoxin test for monoolein-water liquid crystalline gel for use as an implant
Ouédraogo, Moustapha; Semdé, Rasmané; Somé, Issa T. et al

in Tropical Journal of Pharmaceutical Research [=TJPR] (2009), 8(6), 501-508

Purpose : Drugs that are administered by parenteral route must be apyrogenic. The aim of this study was to develop an in vitro endotoxin test for liquid crytalline gels for use as implants, using a ... [more ▼]

Purpose : Drugs that are administered by parenteral route must be apyrogenic. The aim of this study was to develop an in vitro endotoxin test for liquid crytalline gels for use as implants, using a monoolein-water liquid crystalline gel as a model. Methods : The gel-clot technique was used. The gel was dissolved first in isopropyl myristate, and the endotoxins were extracted with water for bacterial endotoxin test. Tests for the labelled lysate sensitivity and interfering factors were performed to validate the developed method. The limit of detection of endotoxin in the gel was also determined. Results : The labelled lysate sensitivity was confirmed. It was not influenced by the presence of extracts from the gels. Endotoxins in the contaminated test gels were completely extracted. Endotoxin concentration in the tested gels was below the calculated threshold endotoxin level. Conclusion : A method to perform in vitro endotoxins test of liquid crystalline gels was successfully developed and validated. Application of the technique to gels currently being developed in our laboratories indicate that the gels were apyrogenic. [less ▲]

Detailed reference viewed: 33 (5 ULg)
See detailDevelopment of an in vitro model for mercury exposure in marine mammals
Dupont, Aurélie ULg; Bouquegneau, Jean-Marie ULg; Siebert, Ursula et al

Poster (2007, November 01)

Detailed reference viewed: 16 (6 ULg)
Peer Reviewed
See detailDevelopment Of An In Vitro Pod Culture Technique For Young Pods Of Phaseolus Vulgaris L.
Geerts, P.; Sassi, K.; Mergeai, Guy ULg et al

in In Vitro Cellular & Developmental Biology-Plant (2000), 36(6),

Detailed reference viewed: 14 (5 ULg)
Full Text
See detailDevelopment of an in vivo phosphorylation system in Escherichia coli
Goux, Marine ULg

Poster (2013, October)

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as zirconium-89 (89Zr, t½=78.4h) or gallium-68 (68Ga, t½=1.13h)[a ... [more ▼]

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as zirconium-89 (89Zr, t½=78.4h) or gallium-68 (68Ga, t½=1.13h)[a]. Despite their important role in the therapeutic world, antibodies have many disadvantages related to their structure. In addition to their limited stability, the presence of disulfide bridges and their post-translational modifications make their production difficult and expensive. Moreover, conjugation of chelating agent often occurs on lysines, which is non-regioselective and leads to a heterogeneous mixture of products. In addition, the long clearance of antibodies can be a problem to obtain a good contrast when they are used in imaging. To address these different limitations, we developed a chemistry-free chelating system consisting of a highly phosphorylatable peptide tag. A specific phosphorylation step, with the alpha subunit of the casein kinase II (CKIIα), generates a nanocluster of 4 phosphates that can interact strongly with metal ion like zirconium. This strategy has already demonstrated its powerfulness for the stable and specific anchoring of protein on zirconium phosphonate-based microarray[b]. We are now adapting the use of this labeling tag to the stereoselective chelation of radionuclides for PET imaging. To ensure an efficient targeting of the radionuclide, the tag was genetically fused to a Nanofitin®, a protein scaffold developed as an alternative to antibodies. Nanofitins share the advantages of being small (7KDa), cystein-free, easy to engineer, highly stable both to temperature and pH and expressed with high yield in Escherichia coli. As described previously, functionalizing the tag with a phosphate nanocluster is a key step to provide it with the capacity to specifically chelate metal ions such as zirconium or gallium. In early development the phosphorylation was realized in vitro. In order to save purification steps and decrease production costs, we developed an in vivo phosphorylation system consisting in the co-expression of a Nanofitin® fused to the phosphorylatable tag and the CKIIα. Effectiveness of the in vivo system was demonstrated together by electrophoretic mobility shift assay and staining with a specific phosphoprotein staining gel: ProQDiamond®. To further optimize and validate the system, the rate of phosphorylation is evaluated by an isoelectric focusing method. This technique is based on the fact that the pHi of the Nanofitin® decreases as the protein is phosphorylated. Such a technique allowed us to isolate the different phosphorylation states of the phospho-Nanofitin® (from 0 to 4 phospho-serine). In parallel to the development of the in vivo system, we are developing a phosphoprotein purification protocol by immobilized metal affinity chromatography, relying on the specific interaction of phosphate moeity with gallium(III) or iron(III). The use of a single tag for both the purification and the labeling will provide a much simpler and straightforward system. Finally this fusion system of phosphate nanocluster and a Nanofitin® will allows us to combine the advantages of an original single step regioselective labeling for both zirconium-89 and gallium-68, together with a highly stable targeting moiety. [a] Heuveling D. A. et al. (2011), Oral Oncology, 47, pp. 2–7 [b] Cinier M. et al. (2012), Journal of Biological Inorganic Chemistry, 17, pp. 399–407 [less ▲]

Detailed reference viewed: 38 (4 ULg)
Full Text
See detailDEVELOPMENT OF AN INTEGRATED STRATEGY FOR CONTROLLING THE ALLERGEN ISSUE IN THE BELGIAN FOOD AND CATERING INDUSTRY - ALLERRISK
De Loose, Marc; Daeseleire, Else; Taverniers, Isabelle et al

Report (2009)

Detailed reference viewed: 33 (2 ULg)