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See detailDevelopment of an Environmental Information System for Odour using Citizen and Technology Innovative Sensors and Advanced Modelling
Uhrner, Ulrich; Grosso, gioavanna; Romain, Anne-Claude ULg et al

in CEUR Workshop Proceedings (2014), 1322

The challenge of OMNISCIENTIS is to develop a community based odour monitoring and information system to mitigate odour annoyance and to foster citizens’ participation in environmental governance. The ... [more ▼]

The challenge of OMNISCIENTIS is to develop a community based odour monitoring and information system to mitigate odour annoyance and to foster citizens’ participation in environmental governance. The core is an information system collecting various data of odour emissions obtained by electronic noses and other sensors, meteorological conditions and observations by citizens acting as human sensors. A specific odour dispersion model is developed to use all this information and provide immediate feedback to all stakeholders. This work presents the architecture of the environmental information system, some first results from odour monitoring and model development and validation. [less ▲]

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See detailDevelopment Of An Enzymatic Assay For The Determination Of Cellulose Bioavailability In Municipal Solid Waste
Rodriguez, Christian; Hiligsmann, Serge ULg; Ongena, Marc ULg et al

in Biodegradation (2005), 16(5), 415-422

As there is a constant need to assess the biodegradation potential of refuse disposed of in landfills, we have developed a method to evaluate the biodegradability of cellulosic compounds (cellulose and ... [more ▼]

As there is a constant need to assess the biodegradation potential of refuse disposed of in landfills, we have developed a method to evaluate the biodegradability of cellulosic compounds (cellulose and hemicellulose) in municipal solid waste. This test is based on the quantification of monosaccharids released after the hydrolysis of solid waste samples with an optimised enzyme preparation containing commercially available cellulases and hemicellulases. We show that the amounts of monosaccharids could be related to the biodegradability of the cellulosic material contained in the samples. This enzymatic cellulose degradation test was assayed on 26 samples originating from two Belgian landfills and collected at different depths. As results correlated well with those obtained with a classical biochemical methane potential assay, this new and rapid test is sufficiently reliable to evaluate cellulose bioavailability in waste samples. [less ▲]

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See detailDevelopment of an enzyme-linked immunosorbent assay for equine neutrophil elastase measurement in blood: Preliminary application to colic cases.
de la Rebière de Pouyade, Geoffroy ULg; Franck, Thierry ULg; Salciccia, Alexandra ULg et al

in Veterinary immunology and immunopathology (2010)

Equine neutrophil elastase (NE) is a protease released in inflammatory diseases and participating in tissue destruction. To measure NE in horse plasma to assess its role in pathological conditions, we ... [more ▼]

Equine neutrophil elastase (NE) is a protease released in inflammatory diseases and participating in tissue destruction. To measure NE in horse plasma to assess its role in pathological conditions, we purified elastase from equine neutrophils by a double step chromatography and obtained a pure protein of 27kDa, 4kDa smaller than the NE 2A previously purified (Scudamore et al., 1993; Dagleish et al., 1999), which was likely to be NE 2B. We developed an ELISA by using two specific polyclonal antibodies obtained from rabbit and guinea pig. The sandwich complex was detected using a secondary antibody conjugated to alkaline phosphatase. The ELISA showed good precision and accuracy, with intra- and inter-assay coefficients of variation below 10% for equine NE concentrations ranging from 1.875 to 60ng/ml. A stable plasma NE value, unaffected by the delay of centrifugation (over 4h), was obtained with plasma from EDTA anticoagulated blood. The mean value (+/-SEM) measured in 37 healthy horses was 32.53+/-4.6ng/ml. NE level in plasma of horses with colic at the time of admission was significantly higher than in healthy horses. Our results indicate that the ELISA technique we developed to measure plasmatic NE is a powerful tool for studying the role of elastase in equine inflammatory disease. In future, the application will be extended to other equine biological fluids. [less ▲]

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See detailDevelopment of an Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Ringworm Infection in Cattle
Bagut, ET; Cambier, Ludivine ULg; Heinen, Marie-Pierre ULg et al

in Clinical and Vaccine Immunology (2013), 20(8), 1150-1154

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See detailDevelopment of an enzyme-linked immunosorbent assay for specific equine neutrophil myeloperoxidase measurement in blood
Franck, Thierry ULg; Grulke, Sigrid ULg; Deby-Dupont, Ginette et al

in Journal of Veterinary Diagnostic Investigation (2005), 17(5), 412-419

Equine inflammatory disease is accompanied by a neutrophil activation resulting in the release of granulocytic enzyme myeloperoxidase (MPO). To measure MPO in horse plasma as marker of neutrophil ... [more ▼]

Equine inflammatory disease is accompanied by a neutrophil activation resulting in the release of granulocytic enzyme myeloperoxidase (MPO). To measure MPO in horse plasma as marker of neutrophil activation, the authors purified equine neutrophil MPO and developed a specific enzyme immunoassay using 2 specific polyclonal antibodies obtained from rabbit (primary antibody) and guinea pig (secondary antibody). The sandwich complex "primary antibody-MPO-secondary antibody" was detected using a goat anti-guinea pig immunoglobulin antibody conjugated to alkaline phosphatase. The enzyme-linked immunosorbent assay (ELISA) showed good precision and accuracy, with intra- and interassay coefficients of variation below 10% for MPO concentrations ranging from 0.78 to 50 ng/ml. A stable plasma MPO value, unaffected by time elapsed between blood collection and centrifugation, was obtained with plasma from EDTA anticoagulated blood. The mean MPO value measured in 38 healthy horses was 181.80 +/- 64.74 ng/ml. In 20 horses suffering from obstruction of the large or small intestine, MPO concentrations measured at the time of arrival at the intensive care unit were significantly higher than mean normal value, ranging from 477.88 to 2,748.13 ng/ml. Work is in progress to apply this MPO ELISA technique to other biological fluids and other equine diseases. [less ▲]

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See detailDevelopment of an Evidence-Based Calibration Methodology Dedicated to Energy Audit of Office Buildings. Part 1: Methodology and Modeling.
Bertagnolio, Stéphane ULg; Andre, Philippe ULg

in Proceedings of the 10th REHVA World Congress - Clima 2010 (2010, May)

1 Introduction To promote improvements in the HVAC installations of existing buildings, the article 9 of the EPBD directive establishes mandatory audits and inspections of air-conditioning systems. The ... [more ▼]

1 Introduction To promote improvements in the HVAC installations of existing buildings, the article 9 of the EPBD directive establishes mandatory audits and inspections of air-conditioning systems. The development of auditing tools and procedures and the training of future auditors are the main objectives of the HARMONAC project launched in 2007. Four audit stages are generally distinguished: benchmarking, inspection, detailed audit and investment grade audit. Answering the questions encountered along the audit process requires some diagnosis, which has to be established on the basis of the very scarce information currently available: technical data contained in as-built files actually available and, generally, very global recordings of energy consumptions. A dynamic hourly building energy simulation (BES) model is being developed in the frame of the HARMONAC project to help the auditor in establishing his diagnosis despite of the lack of data. Two simulation tools dedicated to assist the auditor are developed on the basis of this equation-based BES model: - The first tool is dedicated to a preliminary benchmarking of the installation. - The second tool is used from inspection stage to evaluation of selected energy conservation measures (ECMs). 2 Simulation-based audit methodology The first step of the audit methodology consists in gathering the as-built and utility data, applying a first treatment and analysis method to this data and using the BES model to perform benchmarking. For this benchmarking targeted work, the actual building is coupled to a typical HVAC system whose performances are defined according to EPBD standards. If the benchmarking indicates that potential energy savings should be studied in the building, the calibration-based audit procedure is launched. In the second step, the BES model is integrated in an iterative evidence-based calibration method adapted to audit purposes. The calibration starts directly at the beginning of the inspection, before realizing any additional measurement. This calibration process includes sensitivity and measurement issues, in order to help the auditor identifying critical points and understanding the building operation. Graphical and statistical methods are used in order to quantify and evaluate if the calibrated baseline model satisfies the predefined tolerances. Finally, the calibrated baseline model can be used to evaluate ECMs and savings uncertainty. The development and the implementation of the BES model are presented in this paper. The mains issues of the proposed calibration-based audit methodology are discussed. An example of application of the whole methodology (from benchmarking to evaluation of ECMs) is presented in a companion paper. [less ▲]

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See detailDevelopment of an Evidence-Based Calibration Methodology Dedicated to Energy Audit of Office Buildings. Part 2: Application to a typical office building in Belgium
Andre, Philippe ULg; Fabry, Bertrand ULg; Bertagnolio, Stéphane ULg et al

in Proceedings of the 10th REHVA World Congress - Clima 2010 (2010, May)

The development of specific tools for the audit of air-conditioning systems is described in a companion paper. These developments are carried out in the frame of the HarmonAC project launched in 2007 in ... [more ▼]

The development of specific tools for the audit of air-conditioning systems is described in a companion paper. These developments are carried out in the frame of the HarmonAC project launched in 2007 in order to assist the implementation of the EPBD, article 9. The development of these tools was continously supported by the application to case studies. This paper reports on the application of the tools within an audit procedure applied to a typical office building in Belgium, which is in operation for more than 20 years. The first step of the work consisted in analyzing the current performance of the building which proved to point out a number of problems in the operation of the building. As it is not rarely the case for running office buildings, these problems were due to a lack of commissioning and a progressive decrease of the performances due to a poor management. Application of the first stage “Benchmark” tool to this building led to a very high discrepancy between the measured consumptions and the calculated ones. This appeared mainly for the electricity consumption but also for the gas consumption. Calibration of the simulation tool, mainly by representing more exactly the management of the building and the real internal gains improved the accuracy of the calculations to a large extent. After calibration of the building and HVAC model, the application of a second tool (SimAudit) allowed to evaluate the savings obtained by the implementation of a number of Energy Conservation Opportunities (ECOs). In this case, the dominant ECOs were in relation with the operation of the building: change of schedules, modification of setpoints; implementation of free-cooling strategies; provision of heat recovery and move to reversibility of the chillers. Reasonable estimates of the savings can be obtained following the calibration/simulation process. The paper will highlight some of the most relevant ECOs and provide an order of magnitude for the savings in this kind of building and in a mild climate. [less ▲]

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See detailDevelopment of an ex-vitro system allowing plant-bacteria interactions through VOCs in the context of water stress
Mendaluk, Magdalena ULg; Baudson, Caroline ULg; Delory, Benjamin ULg et al

Poster (2014, February 07)

Water stress is one of the major environmental factors limiting the crop productivity . Plant stress responses are very complex and drought tolerance may be linked to the presence of specific ... [more ▼]

Water stress is one of the major environmental factors limiting the crop productivity . Plant stress responses are very complex and drought tolerance may be linked to the presence of specific microorganisms in the rhizosphere. Indeed, some plant growth promoting bacteria (PGPR) strains have been found to improve plant growth under abiotic stresses. Among the many mechanisms by which those PGPR can support plant growth, the emission of volatile organic compounds (VOCs) and their biological impact are still under study. The aim of this work is to evaluate the interaction between the model grass Brachypodium distachyon (Bd21) and two strains of PGPR. The impact of volatile emission on Bd21 growth was studied using an ex-vitro cocultivation system without physical contact between plant and bacteria during 10 days. This peculiar system was developed to assess bacterial VOCs impacts on plants under realistic growth and stress conditions. In parallel, the response of Bd21 seedlings to water deficit induced by polyethylene glycol 6000 (PEG 6000) was studied to establish contrasted growth conditions regarding water availability [less ▲]

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See detailDevelopment of an experimental device allowing plant-plant interaction studies and in situ dynamic trapping of volatile organic compounds emitted by barley (Hordeum distichon L.) roots
Delory, Benjamin ULg; Delaplace, Pierre ULg; Fauconnier, Marie-Laure ULg et al

in Communications in Agricultural and Applied Biological Sciences (2013, February 08), 78(1), 97-102

In response to wounding or herbivore attack, leaves and roots of higher plants release volatile organic compounds (VOCs). To date, VOCs analysis and plant−plant interaction studies have been mainly ... [more ▼]

In response to wounding or herbivore attack, leaves and roots of higher plants release volatile organic compounds (VOCs). To date, VOCs analysis and plant−plant interaction studies have been mainly performed on aboveground plant tissues, leaving the roles played by root VOCs in plant−plant interaction unexplored. In this context, this project aims at setting up an original experimental device allowing both dynamic trapping of VOCs emitted by mechanically damaged H. distichon roots and the study of the roles played by root VOCs in intra and interspecific plant−plant interactions. The experimental device consists of Barley seedlings cultivated in closed PTFE reactors filled with wet sand. Before being analysed by gas chromatography−mass spectrometry, root VOCs are trapped via a dynamic system on Tenax cartridges using a charcoal-filtered and humidified air. Preliminary results show that 7 day-old wounded Barley roots emit C9 fatty acid derivatives (E-non-2-enal and nona-2,6-dienal) as major compounds, contrasting with aboveground plant tissues that mainly emit C6 alcohols, aldehydes, and their derivative esters. For plant−plant interaction studies, receiver plants are exposed to an airflow enriched with VOCs from root damaged Barley plants of the same age. [less ▲]

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See detailDevelopment of an experimental equipment for the identification of constitutive laws
Flores, Paulo; Rondia, Etienne ULg; Habraken, Anne ULg

in International Journal of Forming Processes (2005), (Special Issue), 117-137

An experimental testing equipment for sheet samples is built in order to identify material parameters ofcomplex phenomenological constitutive laws. This equipment consists in a bi-axial test machine able ... [more ▼]

An experimental testing equipment for sheet samples is built in order to identify material parameters ofcomplex phenomenological constitutive laws. This equipment consists in a bi-axial test machine able to perform plane strain and simple shear tests separately or simultaneously and a Miyauchi simple shear test device. An optical extensometer is used to identify the strain field. The article is focused on the validation of the results of this new equipment by comparing with results obtained by finite elements (FE) simulations of already identified materials. The results are satisfactory and the bases for further material identification are established. [less ▲]

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See detailDevelopment of An Expert System for the Identification of Nonlinear Vibrating Systems
Dimitriadis, Grigorios ULg; Vio, Gareth Arthur; Shi, Dongfeng

in Brennan, M. J.; Liguore, S.; Mace, B. R. (Eds.) et al Proceedings of the IX International Conference on Recent Advances in Structural Dynamics (2006, July)

The aim of the present work is to attempt to create a logical framework to be used for the identification of nonlinear systems. It is assumed that no single identification method is general enough to work ... [more ▼]

The aim of the present work is to attempt to create a logical framework to be used for the identification of nonlinear systems. It is assumed that no single identification method is general enough to work with a significant range of systems. Therefore, this framework is based on the development of an Expert System that will detect and quantify the nonlinearity present in a given dynamical system and, subsequently, determine and apply the most suitable nonlinear system identification method or methods. [less ▲]

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See detailDevelopment of an extended Drucker-Prager hardening model for cross-anisotropy of soft rocks
François, Bertrand; Collin, Frédéric ULg; Dizier, Arnaud et al

in Pietruszczak, S.; Pande, G.N. (Eds.) PROCEEDINGS OF THE 2 nd INTERNATIONAL SYMPOSIUM ON COMPUTATIONAL GEOMECHANICS (COMGEO II) (2011)

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See detailDevelopment of an FDS-SAFIR interface
Tondini, Nicola; Franssen, Jean-Marc ULg

Scientific conference (2011, November 15)

A weak coupling strategy from CFD models to F.E. models is being developed on the base of an interface file. The software FDS and SAFIR are used in the applications.

Detailed reference viewed: 76 (6 ULg)
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See detailDevelopment of an immobilisation device for treatment of patients after breast conserving surgery for mammary carcinoma.
COUCKE, Philippe ULg; WONNER, Michel ULg; JANVARY, Zsolt Levente ULg et al

Poster (2010, September)

To develop an immobilisation device for prone positioning for breast irradiation. The basic requirements for the device are: rapid and accurate patient (re)positioning, compatibility with modern imaging ... [more ▼]

To develop an immobilisation device for prone positioning for breast irradiation. The basic requirements for the device are: rapid and accurate patient (re)positioning, compatibility with modern imaging devices and easy to handle by the treating technologists. [less ▲]

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See detailDevelopment of an implantable integrated MEMS pressure sensor using polyimide epoxy composite and Atomic Layer Deposition for encapsulation
Gembaczka, P; Görtz, M; Kraft, Michael ULg

in Proceedings of "48th DGBMT Annual Conf. : Hannover, Germany Octobre 2014" (2014, October)

Detailed reference viewed: 11 (0 ULg)