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See detailEffect Of Drying On Bioremediation Bacteria Properties
Weekers, F.; Jacques, P.; Springael, D. et al

in Applied Biochemistry and Biotechnology (1998), 70-2

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See detailEffect of drying on paedomorphosis in Triturus alpestris
Denoël, Mathieu ULg; Joly, Pierre; Poncin, Pascal ULg

Conference (2000)

Paedomorphosis consists in the retention of larval characters in adult individuals. It has been observed in several species of newts and salamanders. Optimality models were proposed to explain why it ... [more ▼]

Paedomorphosis consists in the retention of larval characters in adult individuals. It has been observed in several species of newts and salamanders. Optimality models were proposed to explain why it occurs in a large range of aquatic habitats. These models predict that paedomorphs can be advantaged in two situations as contrasted as favourable habitats (paedomorph advantage) or harsh habitats where the newts can benefit of delaying metamorphosis provided that competition with metamorphosed individuals is relaxed (best-of-a-bad-lot hypothesis). This last tactic implies that the habitats are stable enough to ensure the survival of individuals that can not leave the pond. In this respect, our aim was to describe behaviour of paedomorphic Alpine newts (Triturus alpestris) dealing with lake drying. We studied a population from an Alpine lake that splits into two parts during summer, one of them drying then totally. We analyzed age structures by means of skeletochronology and capture-marking-recapture. Paedomorphs and metamorphs showed similar age structures with sexual maturity occurring at 4-5 years, suggesting that most paedomorphs did not metamorphose. CMR data revealed that a great proportion of the paedomorphs marked in the drying part of the lake did not metamorphose and survived to drying. We also carried out laboratory experiments using two-compartiments aquaria to simulate lake drying. These experiments proved that paedomorphic newts could cross terrestrial habitats to reach a pond. Thus paedomorphic newts can migrate on land while keeping their larval attributes rather than metamorphosing when their pond is drying up. Such a tactic appears adaptive because polymorphism in such a deep lake reduces competition between individuals (pers. obs.). [less ▲]

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See detailEffect of duodenal ornithine infusion in cows on milk production and blood plasma constituents at two levels of feeding
Teller, E.; Roets, E.; Godeau, Jean-Marie ULg et al

in Journal of Animal Physiology & Animal Nutrition (1988), 59

An ornithine solution, corresonding to 36.1 g ornithine/d, or an equivalent blank solution were each continuously infused into the duodenum of two lactating cows of the Friesian breed. The cows were fed ... [more ▼]

An ornithine solution, corresonding to 36.1 g ornithine/d, or an equivalent blank solution were each continuously infused into the duodenum of two lactating cows of the Friesian breed. The cows were fed either on a high energy and high protein diet (110% of energy and protein requirements) or a low energy and low protein diet (75% of requirements). Milk composition and milk yield were recorded, and blood plasma contents of amino acids, glucose, growth hormone (GH) and insulin were analysed. The procedure was repeated 1 year later. Ornithine had no effect on milk yields or blood components when thehigh energy and high protein diets were given. On the contrary, it significantly increased milk, lactose, and fat production with the low energy and low protein diets, while milk protein secretion was slightly affected. The levels of nonessential and essential amino acids in blood plasma were enhanced, except for histidine and methionine. The latter amino acids likely were limiting for milk protein synthesis. An increase in glucose supply in the underfed animals likely occured after ornithine administration, since there was a significant increase of plasma glucose and protein levels and total lactose production. Ornithine infusion increased the GH secretion only when the low energy and low protein diets were given. It was concluded that infusion of the non-casein amino acid ornithine into the duodenum increases milk secretion in underfed cows, and that GH likely is involved in this phenomen. [less ▲]

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See detailEffect of duration and temperature of previous vacuum-packed storage on the microbiological quality of Belgian Blue meat packed in high-oxygen atmosphere
Didimo Imazaki, Pedro Henrique ULg; Tahiri, Assia ULg; Daube, Georges ULg et al

Poster (2012, September 05)

The aim of this experiment was to study the effect of duration and temperature of previous vacuum-packed (VP) storage on the microbiological quality of Belgian Blue (BB) beef packed in high-oxygen ... [more ▼]

The aim of this experiment was to study the effect of duration and temperature of previous vacuum-packed (VP) storage on the microbiological quality of Belgian Blue (BB) beef packed in high-oxygen atmosphere. VP striploins from bulls (B) and cows (C) were stored at −1 °C and +4 °C for up to 80 days. These meats were subsequently repackaged under modified atmosphere (MA) – 70 % O2/30 % CO2 – at different times, and stored 2 d at +4 °C and 5 d at +8 °C. The average initial counts in VP meats were 3.6 log CFU/cm² (B) and 2.7 log CFU/cm² (C) for total viable count (TVC) at +22 °C; < 2.0 log CFU/cm² (B and C) for lactic ac id bacteria (LAB) at +22 °C; 1.1 log CFU/cm² (B) and 1.3 log CFU/cm² (C) for Enterobacteriaceae at +30 °C and < 1.0 log CFU/cm² (B and C) for Pseudomonas spp. and Brochothrix thermosphacta. During the first 40 days of VP storage, temperature had a striking influence on microbial growth. The maximum count differences between storage temperatures were obtained at the 20th day of storage: 2.7 log CFU/cm² (B) and 2.9 log CFU/cm² (C) for TVC, 4.0 log CFU/cm² (B and C) for LAB and 3.6 log CFU/cm² (B and C) for Enterobacteriaceae. The difference in TVC between temperatures at the 20th day tended to disappear once the meats were repacked under MA and stored during seven days. Conversely, the difference in LAB and Enterobacteriaceae counts tended to be maintained after MA repackaging, showing that duration and temperature of VP storage had influence on microbiological quality of BB meat subsequently stored in high-oxygen atmosphere. Moreover, chilling at temperatures very close to the freezing point of meat during VP storage, which has already showed innumerous advantages for physicochemical quality of meat, was capital to maintain the microbiological quality of BB fresh meat during subsequent MA-packed storage. [less ▲]

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See detailEffect of duration and temperature of previous vacuum-packed storage on the oxidative stability of Belgian Blue meat packed in high-oxygen atmosphere
Didimo Imazaki, Pedro Henrique ULg; Tahiri, Assia ULg; Thimister, Jacqueline ULg et al

Poster (2012, August 13)

The aim of this experiment was to study the effect of duration and temperature of previous vacuum-packed (VP) storage on the oxidative stability of Belgian Blue meat packed in high-oxygen atmosphere. VP ... [more ▼]

The aim of this experiment was to study the effect of duration and temperature of previous vacuum-packed (VP) storage on the oxidative stability of Belgian Blue meat packed in high-oxygen atmosphere. VP striploins from bulls and cows were stored at −1 °C and +4 °C for up to 80 days and analyzed. These same meat samples were also repackaged under modified atmosphere (MA) – 70 % O2/30 % CO2 – at different times, stored 2 d at +4 °C and 5 d at +8 °C, and then analyzed. Meat from cows presented a lower loss of redness than meat from bulls. A low lipid oxidation was observed in VP samples, but an increase of lipid oxidation took place after MA repackaging. Meat from cows presented a higher -tocopherol content. A decrease of α-tocopherol content during storage was observed as well. The fat content was also higher in meat from cows than in meat from bulls. The duration and temperature of vacuum-packed storage influenced the sensitivity of Belgian Blue beef to pigment and lipid oxidation during subsequent high-oxygen storage. [less ▲]

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See detailEffect of dynamic left ventricular dyssynchrony on dynamic mitral regurgitation in patients with heart failure due to coronary artery disease
LANCELLOTTI, Patrizio ULg; STAINIER, Pierre-Yves ULg; LEBOIS, Florence ULg et al

in American Journal of Cardiology (2005), 96(9), 1304-1307

In patients with heart failure, exercise-induced increases in mitral regurgitation (MR), which convey a poor prognosis, are related to the dynamic distortion of mitral valve geometry. It was hypothesized ... [more ▼]

In patients with heart failure, exercise-induced increases in mitral regurgitation (MR), which convey a poor prognosis, are related to the dynamic distortion of mitral valve geometry. It was hypothesized that dynamic MR may also be related to intermittent changes in left ventricular synchronicity during exercise. (c) 2005 Elsevier Inc. All rights reserved. [less ▲]

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See detailEffect of early feeding on intermediary metabolism of broiler chicks differing in hatching time
Debonne, M.; Witters, A.; Li, Y. et al

in EAAP series (2010)

[No abstract available]

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See detailEffect of Egf on Human Skin Fibroblasts Is Modulated by the Extracellular Matrix
Colige, Alain ULg; Nusgens, Betty ULg; Lapiere, C. M.

in Archives of Dermatological Research (1988), 280(Suppl), 42-6

The aim of this work was to clarify the reason why a discrepancy exists between the effects of epidermal growth factor (EGF) on fibroblasts in culture repressing collagen biosynthesis and in vivo ... [more ▼]

The aim of this work was to clarify the reason why a discrepancy exists between the effects of epidermal growth factor (EGF) on fibroblasts in culture repressing collagen biosynthesis and in vivo stimulating wound healing. The effect of EGF on the biosynthetic activity of fibroblasts was measured in various conditions of cultures: on plastic, on plastic coated with various macromolecules of the extracellular matrix, on top of a type I collagen gel, and within a three-dimensional collagen lattice. While the noncollagen protein (NCP) synthesis was not affected by the interactions of the cell with the various coated matrices, collagen synthesis was inhibited. At the surface of a collagen gel, protein synthesis was reduced, while collagen synthesis and degradation were slightly stimulated. When embedded in a lattice, the overall biosynthetic activity of fibroblasts was largely depressed. The addition of EGF to cultures on plastic and on the various coated macromolecules resulted in a further repression of collagen synthesis while cell multiplication was slightly stimulated. On the contrary, the addition of EGF to fibroblasts in a collagen lattice resulted in a stimulation of both NCP and collagen synthesis as observed in vivo. These opposite effects of EGF in a two- or three-dimensional culture system are not related to modification in number or affinity of the EGF receptors at the cell surface. These results further support the similarity in the state of differentiation of fibroblasts in a three-dimensional lattice and in vivo. [less ▲]

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See detailThe effect of electron-phonon interaction in iron-doped III-V cubic semiconductors
Colignon, David ULg; Kartheuser, Eduard ULg; Villeret, Murielle

in Journal of Physics : Condensed Matter (2000), 12(12), 2691-2699

A theoretical study of optical absorption and emission measurements of Fe2+ as a substitutional impurity in InP and GaP is presented. A new interpretation of the low-temperature absorption spectrum is ... [more ▼]

A theoretical study of optical absorption and emission measurements of Fe2+ as a substitutional impurity in InP and GaP is presented. A new interpretation of the low-temperature absorption spectrum is proposed based on a weak Jahn-Teller interaction between the electronic excited states and a local gap mode of Gamma(5) symmetry. The model also includes the crystal potential, hybridization with the orbitals of the ligands of the host crystal, spin-orbit interaction and a weak dynamic Jahn-Teller coupling of the orbital ground state of Fe2+ with transverse acoustic phonons of Gamma(3) symmetry. The theoretical model describes with good accuracy the measured positions and relative intensities of the spectral lines. In addition, the mass dependence of the local gap mode of Gamma(5) symmetry reproduces the general features of the fine structures associated with the isotopic shifts of the zero-phonon line and the contribution to the isotopic shifts arising from the difference in zero-point energy between the initial and final states of the transition is evaluated. [less ▲]

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See detailEffect of elevated PCO2 on optical properties of the coccolithophorid Emiliania huxleyi grown under nitrate limitation
Denis, M.; Sciandra; Harlay, Jérôme ULg et al

in Cytometry. Part A : The Journal of the International Society for Analytical Cytology (2004), 59A(1), 105

Side scatter and red fluorescence properties of the coccolithophore Emiliania huxleyi were investigated by flow cytometry when NO3-limited continuous cultures were submitted to a CO2 partial pressure ... [more ▼]

Side scatter and red fluorescence properties of the coccolithophore Emiliania huxleyi were investigated by flow cytometry when NO3-limited continuous cultures were submitted to a CO2 partial pressure (pCO2) increase from 400 to 700 ppm. Cultures renewed at the rate of 0.5 d-1 and were submitted to saturating light level. pCO2 was controlled by bubbling CO2-rich or CO2- free air in the cultures. Most of the analyses were repeated 5 times and the average SD were < 1.6%, 0.1 and 0.2% for counting, fluorescence and side scatter respectively. Considering the possible decalcification induced by the increase of CO2 in the chemostat atmosphere, the maximum variation that can be expected for side scatter is that provided by the coccolith depletion upon acidification of the cell suspension. The acidification induced a large (36%) decrease of the side scatter signal but had no detectable effect on the red fluorescence. A control was run with a non-calcifying species, Dunaliella tertiolecta, where acidification induced no detectable change, both on fluorescence and side scatter. During the time of the experiment, the decline of side scatter in chemostat 1 never approached the potential 36% change observed when coccoliths are fully dissolved. Interestingly, the specific chl a fluorescence of E. huxleyi slightly increased during the period of high CO2 level. At the end of the experiment this increase amounted to a significant 2.8% of the initial signal. Furthermore, it progressed linearly with time over the period of observation. However, the experiment did not last enough to know if the fluorescence increase had already reached its maximum value. The acidification experiment supported the use of side scatter as a relevant parameter to trace potential changes in calcification. Since the estimated 25% decrease in calcification induced by the rise in CO2 atmosphere did not result in dramatic changes in side scatter values, we can conclude that the number of cocoliths and the overall shape and granulosity of cells was not significantly affected by this decrease. Changes must have only affected tiny structure details of the coccoliths which is supported by scanning electron microscopy observations. The small but significant increase of the fluorescence signal can be considered as a physiological response to the CO2 rise. This suggests a more dynamic photosynthetic process that would result in a higher rate of organic matter production providing that the system is not nutrient limited as in the present situation. [less ▲]

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See detailEffect of encapsulated nanoparticles on thermophillic anaerobic digestion
Al-Ahmad, Alaa Eddin ULg; Hiligsmann, Serge ULg; Lambert, Stéphanie ULg et al

Poster (2014, February 07)

Recently, enormous interest has been focused on biological applications of metal nanoparticles NPs due to their small size, high specified surface and their great potential in application to many science ... [more ▼]

Recently, enormous interest has been focused on biological applications of metal nanoparticles NPs due to their small size, high specified surface and their great potential in application to many science fields. The most studied process concerns zero valent palladium and iron NPs improving anaerobic biodegradation of chlorinated hydrocarbons (Windt et al., 2005). Moreover, investigation carried out in our lab showed that iron NPs encapsulated in silicate matrix may enhance hydrogen production by Clostridium butyricum (Beckers et al., 2013). Nevertheless the influences of metal NPs on methane producing anaerobic digestion have seldom been investigated. The present work investigates the enhancement effect of seven different metal NPs on methane production during the thermophilic anaerobic digestion. NPs of Cu, Pd, Pt, Ni, Co, Ag and Fe encapsulated in porous silica (SiO2) to prevent their coagulation and agglomeration, were added at concentration of 10-5mol/L in batch test (125ml serum bottles containing 70mL culture medium with 5g/L acetate monohydrate as the sole carbon substrate). Nickel, cobalt and iron NPs improved methane production from acetate. To confirm the previous results, the NPs were tested at different concentrations (10-4, 10-5, and 10-6 mol/L) with starch and glucose substrates. The results show that the impact increases with the increase of NPs concentrations up to 10-4 mol/L. The modified Gompertz equation was applied to describe the effect of NPs on anaerobic digestion. According to this model, the kinetic of methane production was particularly affected by nanoparticles addition. The values of the maximum methane production rate MPR (ml/day) was significantly higher 72.5% with nickel NPs at a concentration of 10-4 mol/L than the control without NPs. [less ▲]

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See detailEffect of end connection restraints on the stability of steel beams in bending
Amara, S.; Kerdal, D. E.; Jaspart, Jean-Pierre ULg

in Advanced Steel Construction (2008)

The influence of the end restraint conditions on the lateral-torsional buckling of beams is investigated in detail using finite element method. The paper focuses on the limitation of Eurocode 3 regarding ... [more ▼]

The influence of the end restraint conditions on the lateral-torsional buckling of beams is investigated in detail using finite element method. The paper focuses on the limitation of Eurocode 3 regarding the lateral bending and torsional restraint coefficients kz and kθ of the end supports. Theoretical expressions of the coefficients kz and kθ taking into account the minor axis flexural restraint at the support and the end torsional restraint respectively are presented. The introduction of new coefficients zk and kθ representing the actual support conditions in the expression of the elastic critical moment is suggested. A comparison between the elastic critical moments for various beam cross-sections, lengths and various end restraints, obtained from the finite-element method, and those derived from EC3 ENV method, in which the proposed coefficients zk and kθ are introduced, confirms the reliability of these coefficients that model the end support conditions. [less ▲]

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See detailEffect of endogenous serotonin on the binding of the 5-HT1A PET ligand 18 F in the rat hippocampus: kinetic b measurements combined with microdialysis
Zimmer, Luc; Mauger, Gweltas; Le Bars, Didier et al

in Journal of Neurochemistry (2002), 80(2), 278-286

By using a combination of an original beta(+)-sensitive intracerebral probe and microdialysis, the effect of increased endogenous serotonin on specific binding of F-18-MPPF [4-(2'-methoxy-phenyl)-1-[2'-[N ... [more ▼]

By using a combination of an original beta(+)-sensitive intracerebral probe and microdialysis, the effect of increased endogenous serotonin on specific binding of F-18-MPPF [4-(2'-methoxy-phenyl)-1-[2'-[N-(2"-pyridinyl)-p-fluorobenzamido]ethyl]piperazine] to the serotonin-1A (5-HT1A) receptors was investigated in the hippocampus of the anaesthetized rat. Our beta-sensitive probe prototype was sensitive enough to obtain Specific F-18-MPPF time-activity curves in the rodent (hippocampus/cerebellum ratio approximate to 2). The serotonin neuronal release was pharmacologically enhanced using fenfluramine at three different doses (1, 2 and 10 mg/kg intravenous) multiplying by 2-15 the extracellular serotonin in the hippocampus. These extracellular variations of extracellular serotonin resulted in dose-ranging decreases in F-18-MPPF-specific binding in the same rat. Our results showed for the first time that F-18-MPPF binding could be modulated by modifications of extracellular serotonin in the rat hippocampus. These results were confirmed by the enhancement of extracellular radioactivity collected in dialysates after the displacement of F-18-MPPF by fenfluramine. After modelization, F-18-MPPF binding could constitute an interesting radiotracer for positron emission tomography in evaluating the serotonin endogenous levels in limbic areas of the human brain. [less ▲]

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See detailEffect of endotoxin insult on critical closure in the canine pulmonary vasculature
D'Orio, Vincenzo ULg; FATEMI, M; MENDES, P et al

in Applied Cardiopulmonary Pathophysiology [=ACP] (1992), 4

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See detailEffect of energy under- and protein normonutrition in the fattening of Belgian Blue double muscled cull cows
Cremer, Véronique; Cabaraux, Jean-François ULg; Hornick, Jean-Luc ULg et al

in Book of abstracts of the 51st Annual Meeting of the European Association for Animal Production (2000)

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See detailEffect of energy under- and protein overnutrition on meat deposition in cull cows
Van Eenaeme, Christian ULg; Hornick, Jean-Luc ULg; Raskin, Pascale et al

in Book of abstracts of the 49th annual meeting of the European association for animal production (1998)

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See detailEffect of Enrofloxacin Therapy on Shipping Fever Pneumonia in Feedlot Cattle
Lekeux, Pierre ULg; Art, Tatiana ULg

in Veterinary Record : Journal of the British Veterinary Association (1988), 123(8), 205-207

The effect of enrofloxacin therapy was investigated in 110 male double-muscled cattle weighing 275 +/- 3 kg, during a spontaneous outbreak of shipping fever occurring 11 +/- 2 days after they arrived in ... [more ▼]

The effect of enrofloxacin therapy was investigated in 110 male double-muscled cattle weighing 275 +/- 3 kg, during a spontaneous outbreak of shipping fever occurring 11 +/- 2 days after they arrived in the feedlot. Forty-six diseased animals were divided randomly into three groups A, B and C, containing 17, 19 and 10 animals, respectively; the animals in group A were injected intramuscularly once daily for three consecutive days with 2.5 mg/kg of enrofloxacin, those in group B with 5 mg/kg of enrofloxacin and those in group C with 10 mg/kg of oxytetracycline. Clinical, serological, production and respiratory functional observations were recorded. The animals were clinically cured after the three day treatment except for three in group A and two in group C. These five animals made a clinical recovery after a three day booster treatment with a dose of 5 mg/kg enrofloxacin. The changes in respiratory gas exchange values induced by shipping fever were completely reversed 15 days later, suggesting that there had been no irreversible lung damage. The daily weight gains and the arterial blood gas values of the three groups of treated cattle were not significantly different. The high efficacy of the low dosage of enrofloxacin in this clinical syndrome may be explained by its antibacterial activity against Pasteurella species and Mycoplasma species. This field trial supports the in vitro studies which suggested than enrofloxacin is an appropriate therapy in cases of shipping fever. [less ▲]

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