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See detailDisrupting the melanin-concentrating hormone receptor 1 in mice leads to cognitive and NMDA response deficit
Grisar, Thierry ULg; Adamantidis, Antoine ULg; Thomas, Elizabeth et al

in Journal of the Neurological Sciences (2005, November 15), 238(Suppl. 1), 288

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See detailDisrupting the melanin-concentrating hormone receptor 1 in mice leads to cognitive deficits and alterations of NMDA receptor function.
Adamantidis, Antoine ULg; Thomas, Elizabeth; Foidart, Agnès ULg et al

in European Journal of Neuroscience (2005), 21(10), 2837-44

In order to investigate the physiological properties of the melanin-concentrating hormone (MCH) we have generated and used mice from which the MCH receptor 1 gene was deleted (MCHR1(Neo/Neo) mice ... [more ▼]

In order to investigate the physiological properties of the melanin-concentrating hormone (MCH) we have generated and used mice from which the MCH receptor 1 gene was deleted (MCHR1(Neo/Neo) mice). Complementary experimental approaches were used to investigate alterations in the learning and memory processes of our transgenic model. The ability of the knockout strain to carry out the inhibitory passive avoidance test was found to be considerably impaired although no significant differences were observed in anxiety levels. This impaired cognitive property prompted us to explore modifications in N-methyl D-aspartate (NMDA) responses in the hippocampus. Intracellular recordings of CA1 pyramidal neurons in hippocampal slices from the MCHR1(Neo/Neo) mice revealed significantly decreased NMDA responses. Finally, using in situ hybridization we found a 15% reduction in NMDAR1 subunit in the CA1 region. These results show for the first time a possible role for MCH in the control of the function of the NMDA receptor. [less ▲]

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See detailDisrupting the wall accumulation of human sperm cells by artificial corrugation
Guidobaldi, H.A.; Jeyaram, Y.; Condat, C.A. et al

in Biomicrofluidics (2015), 9

Many self-propelled microorganisms are attracted to surfaces. This makes their dynamics in restricted geometries very di erent from that observed in the bulk. Swimming along walls is bene cial for ... [more ▼]

Many self-propelled microorganisms are attracted to surfaces. This makes their dynamics in restricted geometries very di erent from that observed in the bulk. Swimming along walls is bene cial for directing and sorting cells, but may be detrimental if homogeneous populations are desired, such as in counting microchambers. In this work, we characterize the motion of human sperm cells 60 um long, strongly confi ned to 25u m shallow chambers. We investigate the nature of the cell trajectories between the con fining surfaces and their accumulation near the borders. Observed cell trajectories are composed of a succession of quasi-circular and quasi-linear segments. This suggests that the cells follow a path of intermittent trappings near the top and bottom surfaces separated by stretches of quasi-free motion in between the two surfaces, as confi rmed by depth resolved confocal microscopy studies. We show that the introduction of arti cial petal-shaped corrugation in the lateral boundaries removes the tendency of cells to accumulate near the borders, an e ffect which we hypothesize may be valuable for micro fluidic applications in biomedicine. [less ▲]

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See detailDisruption in energy metabolism and mitochondrial function in a cellular model of inflammation-induced acute kidney injury
Quoilin, Caroline ULg; Mouithys-Mickalad, Ange ULg; Lécart, Sandrine et al

Poster (2013, September)

Sepsis is a very complex clinical condition characterized by stimulation of a systemic inflammatory response due to an infection. It has a profound deleterious effect on kidney functions leading to sepsis ... [more ▼]

Sepsis is a very complex clinical condition characterized by stimulation of a systemic inflammatory response due to an infection. It has a profound deleterious effect on kidney functions leading to sepsis-induced acute kidney injury (AKI). This failure seems to occur through complex mechanisms involving the immune system response, inflammatory pathways, cellular dysfunction and hemodynamic instability. To study the role of cellular energetic metabolism dysfunction and mitochondrial impairment in the occurrence of AKI during sepsis, we developed an inflammation-induced in vitro model using proximal tubular epithelial cells (HK-2) exposed to a bacterial endotoxin (lipopolysaccharide, LPS). This investigation has provided key features on the relationship between endotoxic stress and mitochondrial respiratory chain assembly defects. Firstly, we have shown that renal cells subjected to LPS are no longer capable to use adequately the available oxygen to maintain their metabolic functions. One hypothesis of this down-regulation suggests that impairment in mitochondria oxidative phosphorylation could prevent cells from using oxygen for adenosine triphosphate (ATP) production and potentially could cause sepsis-induced organ failure. Our study has then investigated this possible mitochondrial impairment to explain the decreased O2 consumption rate observed in LPS-treated HK-2 cells. After exposure to LPS, functionality of mitochondria was affected without any disturbance in their spatial organization. LPS seemed rather to interrupt mitochondrial oxidative phosphorylation by blocking cytochrome c oxidase activity. As a consequence, disruptions in the electron transport and the proton pumping across the system occurred, leading to a decrease of the mitochondrial membrane potential, an electron leakage as the form of superoxide anion, a release of cytochrome c in the cytosol and a decrease in ATP production. This irreversible defect in the production of cellular energy would support the concept that kidney failure in sepsis may occur on the basis of cytopathic hypoxia. [less ▲]

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See detailDisruption of CIC-3, a chloride channel expressed on synaptic vesicles, leads to a loss of the hippocampus
Stobrawa, SM; Breiderhoff, T; Takamori, S et al

in Neuron (2001), 29(1), 185-96

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See detailDisruption of COX-2 modulates gene expression and the cardiac injury response to doxorubicin
Neilan, Tomas; Doherty, Glen; Chen, Gang et al

in American Journal of Physiology - Heart and Circulatory Physiology (2006), 291(2), 532-536

To determine the role of cyclooxygenase (COX)-2 in anthracycline-induced cardiac toxicity, we administered doxorubicin (Dox) to mice with genetic disruption of COX-2 (COX-2−/−). After treatment with Dox ... [more ▼]

To determine the role of cyclooxygenase (COX)-2 in anthracycline-induced cardiac toxicity, we administered doxorubicin (Dox) to mice with genetic disruption of COX-2 (COX-2−/−). After treatment with Dox, COX-2−/− mice had increased cardiac dysfunction and cardiac cell apoptosis compared with Dox-treated wild-type mice. The expression of the death-associated protein kinase-related apoptosis-inducing protein kinase-2 was also increased in Dox-treated COX-2−/− animals. The altered gene expression, cardiac injury, and dysfunction after Dox treatment in COX-2−/− mice was attenuated by a stable prostacyclin analog, iloprost. Wild-type mice treated with Dox developed cardiac fibrosis that was absent in COX-2−/− mice and unaffected by iloprost. These results suggest that genetic disruption of COX-2 increases the cardiac dysfunction after treatment with Dox by an increase in cardiac cell apoptosis. This Dox-induced cardiotoxicity in COX-2−/− mice was attenuated by a prostacyclin analog, suggesting a protective role for prostaglandins in this setting. [less ▲]

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See detailDisruption of PDZ protein-protein interactions inhibits Tax transformation and HTLV-1 infection capacities.
Twizere, Jean-Claude ULg; DEWULF, Jean-François; Blibek, Karim ULg et al

Poster (2011, June 06)

Human T-cell leukemia virus type I (HTLV-1) encodes a Tax oncoprotein that is critical for both viral replication and cellular transformation. HTLV-1 Tax possesses a PDZ domain binding motif (PBM) at its ... [more ▼]

Human T-cell leukemia virus type I (HTLV-1) encodes a Tax oncoprotein that is critical for both viral replication and cellular transformation. HTLV-1 Tax possesses a PDZ domain binding motif (PBM) at its C-terminus that is essential for its transforming activity in a Rat-1 model and for IL-2. Tax has been shown to interact with several PDZ domain containing proteins including PSD-95, Beta1-syntrophin, the precursor of interleukin-16, the mammalian homolog of the Drosophila discs large tumor suppressor protein Dlg, PDLIM2, Lin7, hTid1, Tip1, hScrib and MAGI3. In the 15th International Conference on Human Retrovirology: HTLV and Related Retroviruses, we will present a specificity map for the Tax/PDZ domain interactions generated using the human ORFeome 5.1. and we will focus on some of the new Tax/PDZ interactions. [less ▲]

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See detailLa dissacrazione dei luoghi di culto. Anticlericalismo ne I viceré, I vecchi e i giovani e Il gattopardo
de Seta, Ilaria ULg

in Pirandelliana : Rivista Internazionale di Studi e Documenti (2011), 5

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See detailDissecting the mechanisms underlying old male mating advantage in a butterfly
Karl, Isabell; Heuskin, Stéphanie ULg; Fischer, Klaus

in Behavioral Ecology and Sociobiology (2013)

Selection is expected to maximize an individual’s own genetic reward regardless of the potential fitness consequences for its sexual partners, which may cause sexual conflict. Although performance in ... [more ▼]

Selection is expected to maximize an individual’s own genetic reward regardless of the potential fitness consequences for its sexual partners, which may cause sexual conflict. Although performance in holometabolous insects typically diminishes with age, old male mating advantage has been documented in a few species. Whether this pattern arises from female preference for older males based on, e.g., pheromone blends (intersexual selection), or from increased eagerness to mate in older compared to younger males is currently debated. We explore the mechanistic basis of old male mating advantage, using a series of experiments including behavioral as well as manipulative approaches, in the tropical butterfly Bicyclus anynana. Consistent with the residual reproductive value hypothesis, old male mating advantage was associated with a greater eagerness to mate, evidenced by a two times higher flying and courting activity in older than in younger males. In contrast, we found only limited support for a contribution of female preference for older males based on pheromone composition, although male sex pheromones clearly do play a role in mating success. Our results suggest that male behavior may play a primary role in old male mating advantage, and that pheromones are likely of secondary importance only. Male mating success was related to higher overall pheromone titers rather than variation in a single component. A dominant importance of male behavior in determining mating success may result in sexual conflict. [less ▲]

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See detailLa dissection aigue de l'aorte: la grande simulatrice
Limet, Raymond ULg

in Revue Médicale de Liège (2002), 57(5), 258-62

Dissecting aneurysm is capable to induce death by cardiac complications, internal haemorrhage or acute ischemia of any organ supplied by an aortic branch. Only early and precise diagnosis can oppose the ... [more ▼]

Dissecting aneurysm is capable to induce death by cardiac complications, internal haemorrhage or acute ischemia of any organ supplied by an aortic branch. Only early and precise diagnosis can oppose the fatal evolution. [less ▲]

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See detailLa dissection aortique. Des symptômes a l'indication opératoire
Defraigne, Jean-Olivier ULg; Limet, Raymond ULg

in Revue Médicale de Liège (1996), 51(6), 411-25

La formation d'un hématome au sein de la média aortique à partir d'un orifice d'entrée définit la dissection aortique qui se caractérise par la présence de deux lumières aortiques: la vraie et la fausse ... [more ▼]

La formation d'un hématome au sein de la média aortique à partir d'un orifice d'entrée définit la dissection aortique qui se caractérise par la présence de deux lumières aortiques: la vraie et la fausse. Toute altération structurelle de la média (hypertension, médianécrose, maladie de Marfan, etc...) favorise la dissection. Le début de l'affection est brutal, avec un tableau polymorphe fait de douleurs intenses et migrantes, d'abolition du pouls dans certains territoires, voire d'une insuffisance aortique d'installation rapide. A partir des signes cliniques, le diagnostic est confirmé par des examens complémentaires (échographie, CT-scanner, résonance magnétique nucléaire, artériographie). L'échographie réalisée par voie transoesophagienne tend actuellement à constituer l'examen de premier choix. Dans les dissections qui concernent l'aorte ascendante (types 1, II et III rétrograde de DeBakey, ou type A de Stanford), une chirurgie rapide est impérative en raison de la fréquence des complications mortelles (rupture dans le péricarde avec tamponnade, ischémie myocardique par dissection des coronaires, insuffisance aortique massive avec défaillance ventriculaire gauche). L'intervention consiste à remplacer l'aorte ascendante en conservant ou non la valvule aortique; si l'on implante la prothèse artérielle sur l'annulus aortique, on doit procéder à la réimplantation des coronaires selon la technique de Bentall ou de Cabrol. En cas de dissection qui respecte l'aorte ascendante et ne concerne que l'aorte thoracique descendante (type III a ou b de DeBakey ou type B de Stanford), un traitement médical est d'abord instauré et la chirurgie, qui consiste à remplacer un segment plus ou moins long de l'aorte descendante, n'est appliquée qu'en cas de complications (rupture imminente avec formation d'un épanchement pleural, persistance des douleurs, ischémie viscérale, hypertension incontrôlable). Le traitement médical a pour but de diminuer les douleurs et repose sur l'induction d'une hypotension contrôlée et d'une dépression de la contractilité myocardique; ceci ralentit la vitesse d'établissement de l'onde de pression artérielle, qui constitue le moteur de la dissection. Ce traitement médical doit être instauré avant la chirurgie, après la chirurgie s'il y a lieu de la réaliser, et au long cours une fois le patient sorti de l'hôpital. [less ▲]

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See detailDissection artérielle cérébrale chez l'enfant
BARREA, Christophe ULg; RAMAEKERS, Vincent ULg; OTTO, Bernard ULg et al

Conference (2014, March)

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See detailDissection coronaire et traumatisme thoracique ferme
Brasseur, Edmond ULg; Ghuysen, Alexandre ULg; Mommens, Véronique et al

in Annales de Cardiologie et d'Angeiologie (2006), 55(4), 233-9

The occurrence of an acute myocardial infarction after non-penetrating chest trauma is an extremely rare complication. We report a case of dissection of the left anterior descending artery in a 43 year ... [more ▼]

The occurrence of an acute myocardial infarction after non-penetrating chest trauma is an extremely rare complication. We report a case of dissection of the left anterior descending artery in a 43 year old man after being punched in the chest. Using data from a literature search relish revealed 76 cases; we review the characteristics of the pathology and its incidence. We also characterize its etiology, anatomy and path physiology. We then consider the diagnostic and therapeutic implications. [less ▲]

Detailed reference viewed: 95 (5 ULg)