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See detailDynamics of livestock production systems during the economic transformation period in Northern Vietnam : case study in Cam Giang District, Hai Duong Province
Han Quang, Hanh ULg; Vu Dinh, Ton; Lebailly, Philippe ULg

in Koonawootrittriron, S.; Suwanasopee, T.; Jattawa, D. (Eds.) et al The 15th AAAP Animal Science Congress "Improving Smallholder and Industrial Livestock Production for Enhancing Food Security, Environment and Human Welfare" : Proceedings, Vol. II. Abstracts (2012)

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See detailThe dynamics of lysozyme from bacteriophage lambda in solution probed by NMR and MD simulations.
Smith, Lorna J.; Bowen, Alice M.; Di Paolo, Alexandre et al

in Chembiochem : a European journal of chemical biology (2013), 14(14), 1780-8

(15) N NMR relaxation studies, analyses of NMR data to include chemical shifts, residual dipolar couplings (RDC), NOEs and H(N) -H(alpha) coupling constants, and molecular dynamics (MD) simulations have ... [more ▼]

(15) N NMR relaxation studies, analyses of NMR data to include chemical shifts, residual dipolar couplings (RDC), NOEs and H(N) -H(alpha) coupling constants, and molecular dynamics (MD) simulations have been used to characterise the behaviour of lysozyme from bacteriophage lambda (lambda lysozyme) in solution. The lower and upper lip regions in lambda lysozyme (residues 51-60 and 128-141, respectively) show reduced (1) H-(15) N order parameters indicating mobility on a picosecond timescale. In addition, residues in the lower and upper lips also show exchange contributions to T2 indicative of slower timescale motions. The chemical shift, RDC, coupling constant and NOE data for lambda lysozyme indicate that two fluctuating beta-strands (beta3 and beta4) are populated in the lower lip region while the N terminus of helix alpha6 (residues 136-139) forms dynamic helical turns in the upper lip region. This behaviour is confirmed by MD simulations that show hydrogen bonds, indicative of the beta-sheet and helical secondary structure in the lip regions, with populations of 40-60 %. Thus in solution lambda lysozyme adopts a conformational ensemble that will contain both the open and closed forms observed in the crystal structures of the protein. [less ▲]

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See detailDynamics of mitotic activity and expression of viral proteins gp51 and p24 of Bovine Leukemia Virus producing cells.
Roussev, Roussi; Polianova, M.; Portetelle, Daniel ULg et al

in Folia Biologica (1993), 39

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See detailDynamics of mononuclear cadmium beta-lactamase revealed by the combination of NMR and PAC spectroscopy.
Hemmingsen, L.; Damblon, Christian ULg; Antony, J. et al

in Journal of the American Chemical Society (2001), 123(42), 10329-35

The two metal sites in cadmium substituted beta-lactamase from Bacillus cereus 569/H/9 have been studied by NMR spectroscopy ((1)H, (15)N, and (113)Cd) and PAC spectroscopy ((111m)Cd). Distinct NMR ... [more ▼]

The two metal sites in cadmium substituted beta-lactamase from Bacillus cereus 569/H/9 have been studied by NMR spectroscopy ((1)H, (15)N, and (113)Cd) and PAC spectroscopy ((111m)Cd). Distinct NMR signals from the backbone amides are identified for the apoenzyme and the mononuclear and binuclear cadmium enzymes. For the binuclear cadmium enzyme, two (113)Cd NMR signals (142 and 262 ppm) and two (111m)Cd PAC nuclear quadrupole interactions are observed. Two nuclear quadrupole interactions are also observed, with approximately equal occupancy, in the PAC spectra at cadmium/enzyme ratios < 1; these are different from those derived for the binuclear cadmium enzyme, demonstrating interaction between the two metal ion binding sites. In contrast to the observation from PAC spectroscopy, only one (113)Cd NMR signal (176 ppm) is observed at cadmium/enzyme ratios < 1. The titration of the metal site imidazole (N)H proton signals as a function of cadmium ion-to-enzyme ratio shows that signals characteristic for the binuclear cadmium enzyme appear when the cadmium ion-to-enzyme ratio is between 1 and 2, whereas no signals are observed at stoichiometries less than 1. The simplest explanation consistent with all data is that, at cadmium/enzyme ratios < 1, the single Cd(II) is undergoing exchange between the two metal sites on the enzyme. This exchange must be fast on the (113)Cd NMR time scale and slow on the (111m)Cd PAC time scale and must thus occur in a time regime between 0.1 and 10 micros. [less ▲]

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See detailDynamics of organic and inorganic carbon across contiguous mangrove and seagrass systems (Gazi bay, Kenya)
Bouillon, Steven; Dehairs, Frank; Velimirov, Branko et al

in Journal of Geophysical Research. Biogeosciences (2007), 112(G02018),

We report on the water column biogeochemistry in adjacent mangrove and seagrass systems in Gazi Bay (Kenya), with a focus on assessing the sources and cycling of organic and inorganic carbon. Mangrove and ... [more ▼]

We report on the water column biogeochemistry in adjacent mangrove and seagrass systems in Gazi Bay (Kenya), with a focus on assessing the sources and cycling of organic and inorganic carbon. Mangrove and seagrass-derived material was found to be the dominant organic carbon sources in the water column, and could be distinguished on the basis of their d13C signatures and particulate organic carbon:total suspended matter (POC/TSM) ratios. Spatially, a distinct boundary existed whereby the dominance of mangrove-derived material decreased sharply close to the interface between the mangrove forest and the dense seagrass beds. The latter is consistent with the reported export of mangrove-derived material, which is efficiently trapped in the adjacent seagrass beds. There were significant net inputs of POC and dissolved organic carbon (DOC) along the Kidogoweni salinity gradient, for which the d13CPOC signatures were consistent with those of mangroves. DOC was the dominant form of organic carbon in both mangrove and seagrass beds, with DOC/POC ratios typically between 3 and 15. Dynamics of dissolved inorganic carbon in the creeks were strongly influenced by diagenetic C degradation in the intertidal mangrove areas, resulting in significant CO2 emission from the water column to the atmosphere. Although highest partial pressure of CO2 (pCO2) values and areal CO2 flux rates were observed in the mangrove creeks, and the water column above the seagrass beds was in some locations a net sink of CO2, most of the ecosystems’ emission of CO2 to the atmosphere occurred in the seagrass beds adjacent to the mangrove forest. The presence of dense seagrass beds thus had a strong effect on the aquatic biogeochemistry, and resulted in trapping and further mineralization of mangrove-derived POC, intense O2 production and CO2 uptake. The adjacent seagrass beds provide a large area with conditions favorable to exchange of CO2 with the atmosphere, thereby limiting export of mangrove-derived organic and inorganic carbon toward the coastal ocean. [less ▲]

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See detailDynamics of PCB transfer from mother to pup during lactation in UK grey seals Halichoerus grypus: differences in PCB profile between compartments of transfer and changes during the lactation period
Debier, Cathy; Pomeroy, Paddy P.; Dupont, Cédric et al

in Marine Ecology-Progress Series (2003), 247

The composition of the PCB mixture which is transferred from mother to pup in grey seals Halichoerus grypus throughout lactation was measured in the successive compartments of transfer: maternal blubber ... [more ▼]

The composition of the PCB mixture which is transferred from mother to pup in grey seals Halichoerus grypus throughout lactation was measured in the successive compartments of transfer: maternal blubber, maternal serum, milk and pup serum. Mother-pup pairs were captured regularly between birth and weaning on the Isle of May, Scotland. The PCB profiles of the different compartments of transfer consisted mainly of congeners 153, 138, 180, 187, 170 and 101, which made up 85% of the total PCB burden. Outer blubber contained higher relative concentrations (R-153) Of hepta-, octa-, and nona-chlorinated congeners (higher chlorinated congeners) than inner blubber, at both early (less than or equal to 5 d) and late (greater than or equal to 11 d) lactation. There was no change in the composition of the outer blubber layer between early and late lactation, while in the inner blubber, there was a slight increase in the R153 of higher chlorinated congeners at late lactation. In maternal serum, profiles changed towards having a higher proportion of higher chlorinated congeners as lactation progressed, but in milk, the PCB pattern stayed constant throughout lactation. Surprisingly, in pup serum profiles also changed towards higher R153 of higher chlorinated congeners at late lactation compared to early lactation, which was different to the pattern observed in milk. In all body compartments, higher proportions of higher chlorinated congeners were associated with higher concentrations of total PCBs. Maternal blubber and pup serum contained a higher R153 of higher chlorinated congeners than milk and, to a lesser extent, maternal serum. Thus biomagnification of the higher chlorinated congeners was recorded in pup serum. [less ▲]

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See detailDynamics of pCO2 and related air-ice CO2 fluxes in the Arctic coastal zone (Amundsen Gulf, Beaufort Sea)
Geilfus, Nicolas-Xavier ULg; Carnat, G.; Papakyriakou, T. et al

in Journal of Geophysical Research. Oceans (2012), 117(C00G10),

We present an Arctic seasonal survey of carbon dioxide partial pressure (pCO2) dynamics within sea ice brine and related air-ice CO2 fluxes. The survey was carried out from early spring to the beginning ... [more ▼]

We present an Arctic seasonal survey of carbon dioxide partial pressure (pCO2) dynamics within sea ice brine and related air-ice CO2 fluxes. The survey was carried out from early spring to the beginning of summer in the Arctic coastal waters of the Amundsen Gulf. High concentrations of pCO2 (up to 1834 matm) were observed in the sea ice in early April as a consequence of concentration of solutes in brines, CaCO3 precipitation and microbial respiration. CaCO3 precipitation was detected through anomalies in total alkalinity (TA) and dissolved inorganic carbon (DIC). This precipitation seems to have occurred in highly saline brine in the upper part of the ice cover and in bulk ice. As summer draws near, the ice temperature increases and brine pCO2 shifts from a large supersaturation (1834 matm) to a marked undersaturation (down to almost 0 matm). This decrease was ascribed to brine dilution by ice meltwater, dissolution of CaCO3 and photosynthesis during the sympagic algal bloom. The magnitude of the CO2 fluxes was controlled by ice temperature (through its control on brine volume and brine channels connectivity) and the concentration gradient between brine and the atmosphere. However, the state of the ice-interface clearly affects air-ice CO2 fluxes. [less ▲]

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See detailDynamics of pentaquarks in constituent quark models: Recent developments
Stancu, Floarea ULg

in AIP Conference Proceedings (2005), 775

Some recent developments in the study of light and heavy pentaquarks are reviewed, mainly within constituent quark models. Emphasis is made on results obtained in the flavor-spin model where a nearly ... [more ▼]

Some recent developments in the study of light and heavy pentaquarks are reviewed, mainly within constituent quark models. Emphasis is made on results obtained in the flavor-spin model where a nearly ideal octet-antidecuplet mixing is obtained. The charmed antisextet is reviewed in the context of an SU(4) classification. [less ▲]

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See detailDynamics of poly((dimethylamino)alkyl methacrylate-block-sodium metha-crylate) micelles. Influence of hydrophobicity and molecular architecture on the exchange rate of copolymer molecules
Creutz, Serge; Van Stam, Jan; De Schrijver, Frans C et al

in Macromolecules (1998), 31(3), 681-689

The dynamic stability of the micellar aggregates formed by amphiphilic block copolymers, i.e., poly((dimethylamino)alkyl methacrylate-b-sodium methacrylate), has been investigated by steady-state ... [more ▼]

The dynamic stability of the micellar aggregates formed by amphiphilic block copolymers, i.e., poly((dimethylamino)alkyl methacrylate-b-sodium methacrylate), has been investigated by steady-state fluorescence spectroscopy and size exclusion chromatography. The dynamics of exchange of block copolymer molecules between the micelles, formed in aqueous solution, depend on a manifold of factors, e.g., alkyl substituent in the hydrophobic block, the relation between the hydrophobic and hydrophilic blocks, and the architecture of the block copolymer. All copolymers investigated show a slow unimer exchange with an exchange rate constant on the order of 10-3 s-1, with a difference of a factor of 20 between the fastest and the slowest exchange. It is possible to tune the exchange rate in a controlled way; for instance, an extension or branching of the alkyl chain slows down the exchange rate. The same effect is observed when the hydrophobic/hydrophilic balance of these copolymers is increased or when either the hydrophilic or the hydrophobic moiety of diblocks is divided into two external blocks, leading to a triblock copolymer. [less ▲]

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See detailDynamics of polysaccharides and transparent exopolymer particles during a coccolithophorid bloom in the Bay of Biscay
Händel, Nicole; Piontek, Judith; Harlay, Jérôme ULg et al

Poster (2009, January 25)

Carbohydrates comprise a large fraction (~30 %) of organic matter in the ocean (Pakulski and Benner, 1994). The production of polysaccarides (CCHO) in seawater is mainly linked to autotrophic processes ... [more ▼]

Carbohydrates comprise a large fraction (~30 %) of organic matter in the ocean (Pakulski and Benner, 1994). The production of polysaccarides (CCHO) in seawater is mainly linked to autotrophic processes. CCHO serve as structural and storage compounds, or are released by exudation, cell lysis or leakage to the dissolved organic matter pool. Concentrations of total hydrolyzable neutral sugars range from 200-800nM and 20-170nM in surface and deep waters, respectively (Benner, 2002). It has been shown that CCHO, in particular acidic polysaccharides, are involved in aggregation processes, e.g. the formation of transparent exopolymer particles (TEP). Recently, it has been suggested that ocean acidification increases TEP production, and therefore potentially enhances particle aggregation (Engel 2002, Mari 2008). Here, we present data on the abundance and composition of neutral and acidic CCHO and on TEP, obtained from field investigations and from CO2-controlled chemostat experiments that were conducted during a coccolithophore bloom in the Bay of Biscay 2006. Based on our findings, we discuss the partitioning of CCHO into gel particles, potential effects of ocean acidification and related consequences for organic matter export. [less ▲]

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See detailDynamics of rRNA transcripts within the nucleolus as revealed with confocal and electron microscopy
Thiry, Marc ULg; O'Donohue, Marie-Christine; Kaplan, Hervé et al

Poster (1999)

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See detailDynamics of rRNA within the nucleolus: a study by confocal and electron microscopy
Thiry, Marc ULg; O'Donohue, M-F; Kaplan, H et al

in Analytical Cellular Pathology : The Journal of the European Society for Analytical Cellular Pathology (1999), 18

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See detailDynamics of skin barrier repair following preconditioning by a biotechnology-driven extract from samphire (Crithmum maritimum) stem cells.
CAUCANAS, Marie ULg; Montastier, Christiane; Pierard, Gérald ULg et al

in Journal of Cosmetic Dermatology (2011), 10(4), 288-93

Background With aging, the barrier repair kinetics following any weakening of the epidermal permeability barrier function is commonly slowed down. Objective To assess the recovery rate of the epidermal ... [more ▼]

Background With aging, the barrier repair kinetics following any weakening of the epidermal permeability barrier function is commonly slowed down. Objective To assess the recovery rate of the epidermal permeability barrier function following controlled stripping and applications of samphire and control formulations. Method In 12 healthy subjects older than 50 years, controlled stratum corneum (SC) strippings were used to increase the transepidermal water loss (TEWL) just above 15 g/m(2) /h. This procedure followed a 14-day skin preconditioning by daily applications of formulations enriched or not with a samphire (Crithmum maritimum) biomass. An untreated skin site served as control. The epidermal permeability repair kinetics was assessed for 14 days by daily measurements of both TEWL and the colorimetric value a*. Results A rapid (96 h) recovery to lower TEWL values was obtained at each of the samphire-preconditioned sites (0.1% serum, 0.05% cream, the serum-cream association, and 0.5% silicone oil). This process was significantly (P < 0.001) faster than that on both the placebo-preconditioned (silicone oil) and the untreated sites. No adverse inflammatory and sensory reactions were recorded. At the sites preconditioned by samphire formulations, the SC moisture (capacitance) was higher at completion of the study compared to inclusion. Conclusions The present experimental pilot study brings some clues supporting a beneficial boosting effect of samphire cell biomass on the kinetics of epidermal permeability barrier repair. [less ▲]

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See detailDynamics of skin barrier repair following topical applications of miconazole nitrate.
Xhauflaire, Emmanuelle ULg; Vroome, Valérie; Cauwenbergh, G. et al

in Skin Pharmacology & Physiology (2006), 19(5), 290-4

The skin barrier function (SBF) is an important aspect of skin biology, particularly in the elicitation of inflammation. The SBF recovery rate after tape stripping and surfactant challenge can be assessed ... [more ▼]

The skin barrier function (SBF) is an important aspect of skin biology, particularly in the elicitation of inflammation. The SBF recovery rate after tape stripping and surfactant challenge can be assessed by measuring the transepidermal water loss (TEWL). Previous clinical studies have shown some inflammatory effect after topical applications of miconazole. The aim of this study was to compare the effect of pastes (petrolatum and 15% zinc oxide) containing or not miconazole nitrate on controlled impaired SBF. Fifteen volunteers were enrolled. In each subject, successive cyanoacrylate skin surface strippings were harvested from 5 sites of the volar forearm until TEWL raised above 15 g/cm(2)/h on all test sites. In addition, one daily soak session with a 0.2% dishwashing liquid further damaged the SBF. Each of the test formulations was applied twice daily for 5 days at two dosages, namely 1 and 2 mg/cm(2), on randomized test sites. Another site remained untreated. TEWL was measured daily for 5 days. A fastened SBF repair was observed on all treated sites, particularly where the largest amount of the products had been applied. A faster SBF recovery rate was obtained at the site receiving the miconazole nitrate paste. We conclude that the occlusive effect of a paste helped mitigate SBF defect. The adjunction of miconazole nitrate improved the efficacy. Copyright (c) 2006 S. Karger AG, Basel. [less ▲]

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See detailDynamics of spontaneous ventricular fibrillation in acutely ischemic pigs
Desaive, Thomas ULg; Janssen, Nathalie ULg; Péters, F. et al

in Europace : European Pacing, Arrhythmias, and Cardiac Electrophysiology : Journal of the Working Groups on Cardiac Pacing, Arrhythmias, and Cardiac Cellular Electrophysiology of the European Society of Cardiology (2006), 8 (supplement 1)

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See detailDynamics of the aurora at Jupiter
Bonfond, Bertrand ULg; Grodent, Denis ULg; Badman, Sarah et al

Conference (2014, February 19)

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See detailDynamics of the auroral bifurcations at Saturn and their role in magnetopause reconnection
Gérard, Jean-Claude ULg; Radioti, Aikaterini ULg; Grodent, Denis ULg

Conference (2013, June)

We summarize recent results obtained with the UVIS instrument on board Cassini. They demonstrate that auroral signatures of magnetic field reconnection events in the flanks of the magnetopause are ... [more ▼]

We summarize recent results obtained with the UVIS instrument on board Cassini. They demonstrate that auroral signatures of magnetic field reconnection events in the flanks of the magnetopause are observed in the UV images collected by UVIS. [less ▲]

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See detailDynamics of the Dictyostelium discoideum mitochondrial proteome during vegetative growth, starvation and early stages of development
Czarna, Malgorzata; Mathy, Grégory ULg; Mac Cord, Allan ULg et al

in Proteomics (2010), 9

In this study a quantitative comparative proteomics approach has been used to analyze the D. discoideum mitochondrial proteome variations during vegetative growth, starvation and the early stages of ... [more ▼]

In this study a quantitative comparative proteomics approach has been used to analyze the D. discoideum mitochondrial proteome variations during vegetative growth, starvation and the early stages of development. Application of 2D-DIGE technology allowed the detection of around 2000 protein spots on each two-dimensional gel with 180 proteins exhibiting significant changes in their expression level. In total, 96 proteins (51 unique and 45 redundant) were unambiguously identified. We show that the D. discoideum mitochondrial proteome adaptations mainly affect energy metabolism enzymes (the Krebs cycle, anaplerotic pathways, the oxidative phosphorylation system and energy dissipation), proteins involved in developmental and signalling processes as well as in protein biosynthesis and fate. The most striking observations were the opposite regulation of expression of citrate synthase and aconitase and the very large variation in the expression of the alternative oxidase (AOX) that highlighted the importance of citrate and AOX in the physiology of the development of D. discoideum. Mitochondrial energy states measured in vivo with MitoTracker Orange CMTMRos showed an increase in mitochondrial membrane polarisation during D. discoideum starvation and starvation-induced development. [less ▲]

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See detailThe dynamics of the guests in filled germanium clathrates
Hermann, Raphaël ULg; Grandjean, Fernande ULg; Keppens, V. et al

in Mater. Res. Soc. Symp. Proc. Vol. 886 (2006)

In the filled gallium-germanium clathrates, R8Ga16Ge30, where R is Ba, Sr, or Eu, the guests are located in two large cages and are weakly bound to the crystalline clathrate framework. The caged guests ... [more ▼]

In the filled gallium-germanium clathrates, R8Ga16Ge30, where R is Ba, Sr, or Eu, the guests are located in two large cages and are weakly bound to the crystalline clathrate framework. The caged guests exhibit a localized "rattling" vibrational mode that provides an efficient mechanism for reducing the thermal conductivity. Inelastic neutron scattering and nuclear inelastic scattering measurements have yielded the phonon density of states in R8Ga16Ge30; the line width of the localized vibrational modes is found to be an important parameter in determining the lattice thermal conductivity. Neutron diffraction studies on R8Ga16Ge30 have shown that the guests in the larger cage are located off-center, and it was proposed that their jumping about the four off-center locations is responsible for the observed glass-like thermal conductivity at temperatures below 10 K. The detection of such slow guest motion is challenging because the typical time and energy scales involved are ca. 4 ns and 1 mu eV, respectively. We have studied the slow europium tunneling dynamics in Eu4Sr4Ga16Ge30 by both Mossbauer and microwave absorption spectroscopy. [less ▲]

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See detailDynamics of the Io UV footprints: short timescale brightness variations
Bonfond, Bertrand ULg; Grodent, Denis ULg; Gérard, Jean-Claude ULg

Conference (2006)

The electromagnetic interaction between Io and Jupiter's magnetic field leads to single or multiple UV spots in both jovian hemispheres. Variations of the multiplicity of the spots and the inter-spots ... [more ▼]

The electromagnetic interaction between Io and Jupiter's magnetic field leads to single or multiple UV spots in both jovian hemispheres. Variations of the multiplicity of the spots and the inter-spots distances have been observed and are linked to the position of Io in its plasma torus. These morphological changes have a timescale of a few hours. We have studied the footprint evolution with a time resolution of a few tens of seconds using the HST/STIS camera in the time-tag mode. This approach resulted in an evaluation of the impact of the rapid evolution of the jovian magnetic field lines when measuring the dimensions of the spots with a longer exposure time. More basically, evidence of strong brightness variations of the main spots (up to 40%) have been observed with a typical growth time of 1 minute. Finally, unexpected simultaneous fluctuations of both primary and secondary spots have also been found in the southern hemisphere. In conclusion, the characterisation of the variations mentioned herein provides clues for a complex and rapidly evolving energetic electrons precipitation mechanism. [less ▲]

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