Dendritic cell differentiation and immune tolerance to insulin-related peptides in Igf2-deficient mice
Hansenne, Isabelle ; ; Greimers, Roland et al
in Journal of Immunology (Baltimore, Md. : 1950) (2006), 176(8), 4651-4657
There is some evidence that insulin-like growth factor 2 (IGF-2) may intervene in the control of T cell differentiation. To further study the immunoregulatory function of this growth factor, we analyzed ... [more ▼]
There is some evidence that insulin-like growth factor 2 (IGF-2) may intervene in the control of T cell differentiation. To further study the immunoregulatory function of this growth factor, we analyzed the immune system of Igf2(-/-) mice. Phenotypically, some immunological parameters such as lymphoid organ morphology and cellularity were unaltered in Igf2(-/-) mice, but an increase of CD8(+) cells and a decrease of B220(+) cells were observed in spleen. In vitro, the development of bone marrow-derived dendritic cells was affected by the absence of Igf2 expression. After maturation, a higher percentage of immature dendritic cells was observed in Igf2(-/-) population, together with a secondary decrease in allogenic T cell proliferation. Activation of T cells was also affected by the lack of expression of this growth factor. The profile of B cell response in mutant mice immunized with IGF-2 evidenced a T-dependent profile of anti-IGF-2 Abs that was absent in Igf2(+/+) mice. The influence of IGF-2 upon tolerance to insulin was also assessed in this model, and this showed that IGF-2 also intervenes in tolerance to insulin. The presence of a T-dependent response in Igf2-deficient mice should allow cloning of specific "forbidden" T CD4(+) lymphocytes directed against IGF-2, as well as further investigation of their possible pathogenic properties against insulin family. [less ▲]Detailed reference viewed: 32 (5 ULg)
Dendritic cells genetically engineered to express IL-10 induce long-lasting antigen-specific tolerance in experimental asthma
; ; et al
Poster (2007)Detailed reference viewed: 13 (1 ULg)
Dendritic cells genetically engineered to express IL-10 induce long-lasting antigen-specific tolerance in experimental asthma.
; Desmet, Christophe ; et al
in Journal of Immunology (2008), 181(10), 7230-7242
Dendritic cells (DCs) are professional APCs that have a unique capacity to initiate primary immune responses, including tolerogenic responses. We have genetically engineered bone marrow-derived DCs to ... [more ▼]
Dendritic cells (DCs) are professional APCs that have a unique capacity to initiate primary immune responses, including tolerogenic responses. We have genetically engineered bone marrow-derived DCs to express the immunosuppressive cytokine IL-10 and tested the ability of these cells to control experimental asthma. A single intratracheal injection of OVA-pulsed IL-10-transduced DCs (OVA-IL-10-DCs) to naive mice before OVA sensitization and challenge prevented all of the cardinal features of airway allergy, namely, eosinophilic airway inflammation, airway hyperreactivity, and production of mucus, Ag-specific Igs, and IL-4. OVA-IL-10-DCs also reversed established experimental asthma and had long-lasting and Ag-specific effects. We furthermore showed, by using IL-10-deficient mice, that host IL-10 is required for mediating the immunomodulatory effects of OVA-IL-10-DCs and demonstrated a significant increase in the percentage of OVA-specific CD4(+)CD25(+)Foxp3(+)IL-10(+) regulatory T cells in the mediastinal lymph nodes of OVA-IL-10-DC-injected mice. Finally, adoptive transfer of CD4(+) mediastinal lymph node T cells from mice injected with OVA-IL-10-DCs protected OVA-sensitized recipients from airway eosinophilia upon OVA provocation. Our study describes a promising strategy to induce long-lasting Ag-specific tolerance in airway allergy. [less ▲]Detailed reference viewed: 72 (15 ULg)
Dendritic cells in Barrett's carcinogenesis: an inadequate environment for antitumor immunity?
Conference (2013)Detailed reference viewed: 25 (8 ULg)
Dendritic cells in Barrett's esophagus carcinogenesis: an inadequate microenvironment for antitumor immunity?
Somja, Joan ; Demoulin, Stéphanie ; RONCARATI, Patrick et al
in American Journal of Pathology (2013), 182Detailed reference viewed: 69 (20 ULg)
Dendritic cells induce the death of human papillomavirus-transformed keratinocytes
Hubert, Pascale ; Giannini, Sandra ; Vanderplasschen, Alain et al
in FASEB Journal (2001), 15(13), 2521-2523Detailed reference viewed: 36 (12 ULg)
Dendritic Cells: More Than Just Adaptive Immunity Inducers?
Jacobs, Nathalie ; Boniver, Jacques ; Hubert, Pascale et al
in Current Immunology Reviews (2007), 2
Dendritic cells (DC) are well known for their capacity to induce immune responses and there is also accumulating evidence of their ability to interact with various cell types of the innate system, such as ... [more ▼]
Dendritic cells (DC) are well known for their capacity to induce immune responses and there is also accumulating evidence of their ability to interact with various cell types of the innate system, such as NK, NKT or TCR gamma-delta cells. These interactions are bi-directional, mediated by soluble or cell surface molecules and have been mainly described in the context of immune responses to infectious agents and tumors. NK, NKT or TCR gamma-delta cells induce the maturation of DC, as shown by the increased expression of CD86, IL12 production and priming of T cell responses. On the other hand, mature DC have the ability to activate NK, NKT or TCR gamma-delta cells for sustained innate immune responses and activated NK cells may kill immature DC. In addition, DC and NK or TCR gamma-delta cells share similar functions such as cytotoxic and antitumor activity, interferon production and antigen presentation capacity. [less ▲]Detailed reference viewed: 40 (7 ULg)
Dendritic cells: potential actors in prion neuroinvasion.
; ; Defaweux, Valérie et al
Poster (2006, September)Detailed reference viewed: 13 (7 ULg)
Dendritic flux penetration in Pb films with a periodic array of antidots
; ; Silhanek, Alejandro et al
in Physical Review. B : Condensed Matter (2005), 71(10),
We explore the flux-jump regime in type-II Pb thin films with a periodic array of antidots by means of magneto-optical measurements. A direct visualization of the magnetic flux distribution allows us to ... [more ▼]
We explore the flux-jump regime in type-II Pb thin films with a periodic array of antidots by means of magneto-optical measurements. A direct visualization of the magnetic flux distribution allows us to identify a rich morphology of flux penetration patterns. We determine the phase boundary H-*(T) between dendritic penetration at low temperatures and a smooth flux invasion at high temperatures and fields. For the whole range of fields and temperatures studied, guided vortex motion along the principal axes of the square pinning array is clearly observed. In particular, the branching process of the dendrite expansion is fully governed by the underlying pinning topology. A comparative study between macroscopic techniques and direct local visualization sheds light onto the puzzling T- and H-independent magnetic response observed at low temperatures and fields. Finally, we find that the distribution of avalanche sizes at low temperatures can be described by a power law with exponent tau similar to 0.9(1). [less ▲]Detailed reference viewed: 12 (1 ULg)
The dendritic organization of the human spinal cord: the dorsal horn.
in Neuroscience (1982), 7(9), 2057-87
The human spinal cord was studied with the Golgi method from 26 weeks gestational age onto adult life. Impregnated neurons were analyzed morphometrically by an adaptation of Sholl's concentric circle ... [more ▼]
The human spinal cord was studied with the Golgi method from 26 weeks gestational age onto adult life. Impregnated neurons were analyzed morphometrically by an adaptation of Sholl's concentric circle method in order to specify dendritic geometry, ramification richness and branching pattern. Neurons were classified according to Rexed's laminar scheme, identified on adjacent Nisslstained sections. The following features were found to be characteristic of the dorsal horn laminae. Lamina I is formed of a uniform population of large, poorly ramified neurons. Their main dendritic domaine is disk-shaped in the tangential plane with a mean diameter of about 800 micrometers. Orthogonal, spiny dendrites penetrating into lamina II are numerous even in adult material. Four cell types, all lying in the sagittal plane, are found in lamina II. (1) "Islet cells' (about 30% of impregnated neurons) have a rostrocaudal, cylindrical dendritic domaine with a long axis of +/- 600 micrometers, a few scattered spines and a richly branched axonal network, confined to the dendritic territory. They are more numerous in the central part of lamina II. A few islet cells have two axons. (2) "Filamentous cells' (about 20%), so called because of their multiple filiform, spiny dendrites, are vertico-sagittally oriented. Their soma is located in inner or outer lamina II, their dendritic tree, trapezoid of about 280 micrometers in height, being dorsal or ventral. The axon emits some collaterals in the vicinity of the dendritic tree, then it penetrates into lamina I or Lissauer's tract. (3) "Curly cells' (about 10%) have a complex, twisted, spine-rich dendritic tree. The dendritic domaine can be schematized by a sagittal disk with a diameter of about 200 micrometers, the cell body being eccentric. Curly cells are mainly found in outer lamina II. Their axon penetrates into lamina I or Lissauer's tract. (4) "Stellate cells' (about 40%) are multipolar neurons preferentially found in inner lamina II. They have straight, spine-poor dendrites, which cover a large (longest diameter ca. 500 micrometers), elliptical territory extending into laminae I and III. Their axon gives longitudinal collaterals to lamina II before penetrating deeply into laminae III and IV. Lamina III contains a mixed population of "antenna-like neurons' with a vertical, cone-shaped dendritic domaine and "radiate cells' characterized by a small, spherical territory. All lamina IV neurons are medium or large sized "antenna-like neurons' whose dorsally oriented, cone-shaped dendritic domaine may have a height of 1000 micrometers. It can be concluded that the dorsal horn of the human spinal cord has several distinct dendroarchitectonic features, different from those reported in animals. The possible functional implication of some dendritic features is examined and a laminar dendroarchitectonic scheme of the human cord is proposed as a morphological tool for future neuroanatomical and neuropathological studies. [less ▲]Detailed reference viewed: 28 (0 ULg)
The dendritic organization of the human spinal cord: the motoneurons.
in Journal of Comparative Neurology (The) (1982), 211(3), 226-47
The dendritic organization of motoneurons was analyzed with the Golgi stain and a morphometric method in the immature and adult human spinal cord. Each motoneuronal column was found to be characterized by ... [more ▼]
The dendritic organization of motoneurons was analyzed with the Golgi stain and a morphometric method in the immature and adult human spinal cord. Each motoneuronal column was found to be characterized by a specific orientation of dendritic trees and by a distinct pattern of dendritic bundling. Ventromedial motoneurons have a pyramidal dendritic tree with numerous, short longitudinal branches and elongated dorsal branches. The latter form thick bundles oriented toward the ventral gray commissure. Longitudinal dendrites form a narrow-meshed dendritic plexus, containing abundant microbundles. Motoneurons of the ventromedial column have fewer primary dendrites and a lower ramification index than other motoneurons. Central motoneurons are predominantly oriented longitudinally. The meshes of the rostrocaudal dendritic plexus are looser and the microbundles are finer. Most transverse dendrites run laterally and participate in dendritic bundles which penetrate into the ventrolateral funiculus. The rostrocaudal dendritic domain of ventrolateral motoneurons is the largest dendritic domain of all spinal neurons. The longitudinal dendritic network contains fine microbundles and appears wide-meshed. Transverse dendrites form lateral or medial dendritic bundles depending upon the position of their perikaryon. Dorsolateral motoneurons differ from other motoneurons by their multipolar organization with a slight preponderance of dorsoventral dendritic spread. Rudimentary lateral dendrite bundles are restricted to marginal neurons. The longitudinal plexuses of motoneuronal dendrites and the verticotransverse dendrite bundles of the ventromedial column are well developed in the 26-28-week-old fetus. In contrast, the horizontotransverse dendrite bundles of central and ventrolateral motoneurons can only be recognized from 36 weeks on. The possible specific functions of the various types of dendrites bundles are examined and a laminar dendroarchitectonic schema of the human cord is proposed. [less ▲]Detailed reference viewed: 27 (0 ULg)
Dendrochronological analysis of large tropical trees: a new approach combining photogrammetry, image processing and GIS tools
Latte, Nicolas ; Bauwens, Sébastien ; Bonnet, Stéphanie et al
Conference (2014, January 17)
Tree-ring analysis of tropical trees is especially time-consuming for the largest trees. Because of large dimensions and irregular shapes, the tree-ring measurements are generally achieved (semi-)manually ... [more ▼]
Tree-ring analysis of tropical trees is especially time-consuming for the largest trees. Because of large dimensions and irregular shapes, the tree-ring measurements are generally achieved (semi-)manually directly from wood samples. The aim of this study is to present a new approach of tree-ring measurements combining photogrammetry, image processing and GIS tools that is appropriate for very large wood samples. Disks are sawed from felled trees above buttress. Small diameters holes are drilled to the disk surface to be later used as control points. Images are taken with an off-the-shelf camera and assembled with PhotoScan in order to produce an orthoimage of the green disk surface. After drying, disks are sanded (grain 80-400) and scanned with a flatbed scanner (A3 size) at high resolution (1200-2400 dpi). The scans are then mosaicked together with Autopano into one single image. Based on the control points of the green orthoimage, the ‘dry’ image is georeferenced and rectified. Using a GIS environment, tree-rings are delimited and ring widths and/or areas measured. Disks are entirely digitalized into high resolution images (0.02-0.04mm per pixel) offering a wide range of analysis possibilities. The image-based procedure reduces cost of disk handling and transportation, facilitates data processing and transfer and, is adaptable to any type of wood samples. [less ▲]Detailed reference viewed: 228 (47 ULg)
Dendrochronological research on beech in Belgium: the case of 12th graves from the Nivelles Abbey (Hainaut) and future archaeological prospects
; Weitz, Armelle ;
in Eurodendro 2014 (2014, September)Detailed reference viewed: 44 (4 ULg)
La dendrochronologie au service de l'histoire de l'Art. Analyse de sculptures du XVIème siècle.
Van Ruymbeke, Muriel
Poster (2006, May 16)Detailed reference viewed: 7 (1 ULg)
La dendrochronologie au service de la datation des monuments
in Bulletin de la Fondation Vanhove-Vonneche (1991), 3Detailed reference viewed: 20 (5 ULg)
La dendrochronologie au service des embarcations gallo-romaines de Pommeroeul
Conference (2013, February 16)Detailed reference viewed: 9 (3 ULg)
La dendrochronologie des panneaux peints: l'exemple d'un plafond peint par Damery à Alden Biesen
in Bulletin de l'Institut Archéologique Liégeois (1989), 101Detailed reference viewed: 27 (1 ULg)
Dendrochronologie en Belgique et régions limitrophes
in Archaeologia mediaevalis (1992), 15
This is a presentation of dendrochronological analysis of Laboratory of ULg during the year 1991 in the following cities: Brussels (B), Enghien (B), Gent (B), Liège (B), Maastricht (Nl), Metz (F), Seneffe ... [more ▼]
This is a presentation of dendrochronological analysis of Laboratory of ULg during the year 1991 in the following cities: Brussels (B), Enghien (B), Gent (B), Liège (B), Maastricht (Nl), Metz (F), Seneffe (B) and Saint-Hubert (B) [less ▲]Detailed reference viewed: 11 (0 ULg)