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See detailDetection of quantitative trait loci affecting non-return rate in French dairy cattle.
Ben Jemaa, S.; Fritz, S.; Guillaum, F. et al

in Journal of Animal Breeding & Genetics (2008), 125(4), 280-8

The purpose of this study was to map quantitative trait loci (QTL) influencing female fertility estimated by non-return rate (NRR) in the French dairy cattle breeds Prim'Holstein, Normande and ... [more ▼]

The purpose of this study was to map quantitative trait loci (QTL) influencing female fertility estimated by non-return rate (NRR) in the French dairy cattle breeds Prim'Holstein, Normande and Montbeliarde. The first step was a QTL detection study on NRR at 281 days after artificial insemination on 78 half-sib families including 4993 progeny tested bulls. In Prim'Holstein, three QTL were identified on Bos taurus chromosomes BTA01, BTA02 and BTA03 (p < 0.01), whereas one QTL was identified in Normande on BTA01 (p < 0.05). The second step aimed at confirming these three QTL and refining their location by selecting and genotyping additional microsatellite markers on a sub-sample of 41 families from the three breeds using NRR within 56, 90 and 281 days after AI. Only the three QTL initially detected in Prim'Holstein were confirmed. Moreover, the analysis of NRR within 56, 90 and 281 days after AI allowed us to distinguish two FF QTL on BTA02 in Prim'Holstein, one for NRR56 and one for NRR90. Estimated QTL variance was 18%, 14%, 11.5% and 14% of the total genetic variance, respectively, for QTL mapping to BTA01, BTA02 (NRR90 and NRR56) and BTA03. [less ▲]

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See detailDetection of Quantitative Trait Loci for reproduction and production traits in Large White and French Landrace pig populations
Tribout, T.; Iannuccelli, N.; Druet, Tom ULg et al

in Proceedings of the 8th World Congress on Genetics Applied to Livestock Production (2006)

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See detailDetection of quantitative trait loci for reproduction and production traits in Large White and French Landrace pig populations.
Tribout, Thierry; Iannuccelli, Nathalie; Druet, Tom ULg et al

in Genetics, Selection, Evolution (2008), 40(1), 61-78

A genome-wide scan was performed in Large White and French Landrace pig populations in order to identify QTL affecting reproduction and production traits. The experiment was based on a granddaughter ... [more ▼]

A genome-wide scan was performed in Large White and French Landrace pig populations in order to identify QTL affecting reproduction and production traits. The experiment was based on a granddaughter design, including five Large White and three French Landrace half-sib families identified in the French porcine national database. A total of 239 animals (166 sons and 73 daughters of the eight male founders) distributed in eight families were genotyped for 144 microsatellite markers. The design included 51 262 animals recorded for production traits, and 53 205 litter size records were considered. Three production and three reproduction traits were analysed: average backfat thickness (US_M) and live weight (LWGT) at the end of the on-farm test, age of candidates adjusted at 100 kg live weight, total number of piglets born per litter, and numbers of stillborn (STILLp) and born alive (LIVp) piglets per litter. Ten QTL with medium to large effects were detected at a chromosome-wide significance level of 5% affecting traits US_M (on SSC2, SSC3 and SSC17), LWGT (on SSC4), STILLp (on SSC6, SSC11 and SSC14) and LIVp (on SSC7, SSC16 and SSC18). The number of heterozygous male founders varied from 1 to 3 depending on the QTL. [less ▲]

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See detailDetection of Quantitative Trait Loci for sow reproduction traits using multivariate analyses
Rosendo, A.; Bidanel, J. P.; Druet, Tom ULg et al

in Proceedings of the 8th World Congress on Genetics Applied to Livestock Production (2006)

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See detailDetection of recurrent colorectal carcinoma with whole-body FDG-PET
DAENEN, Frédéric ULg; HUSTINX, Roland ULg; PAULUS, P. et al

in Journal of Nuclear Medicine (The) (1996, May), 37

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See detailDetection of response to command using voluntary control of breathing in disorders of consciousness
Charland-Verville, Vanessa ULg; Lesenfants, Damien; Sela, Lee et al

in Frontiers in Human Neuroscience (2014), 8(1020),

BACKGROUND: Detecting signs of consciousness in patients in a vegetative state/unresponsive wakefulness syndrome (UWS/VS) or minimally conscious state (MCS) is known to be very challenging. Plotkin et al ... [more ▼]

BACKGROUND: Detecting signs of consciousness in patients in a vegetative state/unresponsive wakefulness syndrome (UWS/VS) or minimally conscious state (MCS) is known to be very challenging. Plotkin et al. (2010) recently showed the possibility of using a breathing-controlled communication device in patients with locked in syndrome. We here aim to test a breathing-based "sniff controller" that could be used as an alternative diagnostic tool to evaluate response to command in severely brain damaged patients with chronic disorders of consciousness (DOC). METHODS: Twenty-five DOC patients were included. Patients' resting breathing-amplitude was measured during a 5 min resting condition. Next, they were instructed to end the presentation of a music sequence by sniffing vigorously. An automated detection of changes in breathing amplitude (i.e., >1.5 SD of resting) ended the music and hence provided positive feedback to the patient. RESULTS: None of the 11 UWS/VS patients showed a sniff-based response to command. One out of 14 patients with MCS was able to willfully modulate his breathing pattern to answer the command on 16/19 trials (accuracy 84%). Interestingly, this patient failed to show any other motor response to command. DISCUSSION: We here illustrate the possible interest of using breathing-dependent response to command in the detection of residual cognition in patients with DOC after severe brain injury. [less ▲]

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See detailDetection of ribosomal DNA and RNA sequences on ultrathin sections by in situ hybridization
Thiry, Marc ULg

in European Journal of Cell Biology (1993), 61

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See detailDetection of ribosomal RNA during the cell cycle in Ehrlich tumour cells by electron microscope in situ hybridization.
Thiry, Marc ULg

in European Journal of Morphology (1993), 31(1-2), 13-6

The fine spatial distribution of rRNA within Ehrlich tumour cells has been investigated during the cell cycle by in situ hybridization with a biotinylated probe. In interphase cells, rRNA was essentially ... [more ▼]

The fine spatial distribution of rRNA within Ehrlich tumour cells has been investigated during the cell cycle by in situ hybridization with a biotinylated probe. In interphase cells, rRNA was essentially detected in the ribosome-rich cytoplasmic regions and in the three main nucleolar components. In mitotic cells, besides the presence, as expected, of rRNA in the ribosome-rich spaces, evident label was observed over material situated around and between the chromosomes during anaphase and telophase as well as over the reforming nucleoli during telophase. [less ▲]

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See detailDetection of RNA on ultrathin sections incubated with poly A polymerase, as visualized by an immunogold labeling procedure
Thiry, Marc ULg

in Archives Internationales de Physiologie et de Biochimie (1992), 100

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See detailDetection of rRNA synthesis sites within reptilian nucleoli
Bartholomé, Odile ULg; Thiry, Marc ULg; Franck, Claire

Poster (2014, December 13)

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See detailDetection of small amounts of human adenoviruses in stools: comparison of a new immuno real-time PCR assay with classical tools.
Bonot, Sebastian; Ogorzaly, Leslie; Elmoualij, Benaïssa ULg et al

in Clinical Microbiology & Infection (2014), 20(12), 1-7

The detection of low virus concentrations in biological matrices, especially stool samples, is facing significant limitations as far as common diagnostic methods (enzyme-linked-immunosorbent assay (ELISA ... [more ▼]

The detection of low virus concentrations in biological matrices, especially stool samples, is facing significant limitations as far as common diagnostic methods (enzyme-linked-immunosorbent assay (ELISA) or quantitative real-time PCR (qPCR)) are considered. Here the development of a new immuno real-time PCR (iPCR) is described and its performance in the detection of human adenoviruses (HAdVs) in spiked stools is compared with those of ELISA and qPCR assays. For the iPCR, detection of the sandwich formed by the complexation of capture antibody-antigen-detection antibody was performed by qPCR thanks to the substitution of peroxydase by a chimeric DNA. This modification increased the detection sensitivity 200-fold compared to ELISA. The direct qPCR results revealed that only 0.3–9.5% of the spiked HAdV were detectable, resulting from important losses of DNA occurring at the extraction step. This step was not necessary in the iPCR workflow, avoiding this drawback. The losses of viral particles occurred at the elution step from the stool only. The recovery rate of the iPCR was thus better and ranged between 21 and 54%. As a result, iPCR enabled the detection of lower virus concentrations in stool samples compared to those detected by ELISA and qPCR. The iPCR could be considered as a ‘hyper sensitive ELISA’ for early detection of HAdV infections, especially in the case of immunocompromised patients after haematopoietic stem cell transplant. [less ▲]

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See detailDetection of small-scale structures in the neutral atmosphere using double differences of GNSS measurements
Brenot, Hugues; Lejeune, Sandrine; Warnant, René ULg

Conference (2008)

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See detailDetection of Solar-like Oscillations from Kepler Photometry of the Open Cluster NGC 6819
Stello, Dennis; Basu, Sarbani; Bruntt, Hans et al

in Astrophysical Journal Letters (2010), 713

Asteroseismology of stars in clusters has been a long-sought goal because the assumption of a common age, distance, and initial chemical composition allows strong tests of the theory of stellar evolution ... [more ▼]

Asteroseismology of stars in clusters has been a long-sought goal because the assumption of a common age, distance, and initial chemical composition allows strong tests of the theory of stellar evolution. We report results from the first 34 days of science data from the Kepler Mission for the open cluster NGC 6819—one of the four clusters in the field of view. We obtain the first clear detections of solar-like oscillations in the cluster red giants and are able to measure the large frequency separation, Δν, and the frequency of maximum oscillation power, ν[SUB]max[/SUB]. We find that the asteroseismic parameters allow us to test cluster membership of the stars, and even with the limited seismic data in hand, we can already identify four possible non-members despite their having a better than 80% membership probability from radial velocity measurements. We are also able to determine the oscillation amplitudes for stars that span about 2 orders of magnitude in luminosity and find good agreement with the prediction that oscillation amplitudes scale as the luminosity to the power of 0.7. These early results demonstrate the unique potential of asteroseismology of the stellar clusters observed by Kepler. [less ▲]

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See detailDetection of specific collagen types in normal and keratoconus corneas.
Newsome, D. A.; Foidart, Jean-Michel ULg; Hassell, J. R. et al

in Investigative Ophthalmology & Visual Science (1981), 20(6), 738-50

Keratoconus is a corneal disease of unknown cause that involves a progressive thinning and scarring of the corneal connective tissue. We examined normal human and keratoconus corneas, including one healed ... [more ▼]

Keratoconus is a corneal disease of unknown cause that involves a progressive thinning and scarring of the corneal connective tissue. We examined normal human and keratoconus corneas, including one healed penetrating keratoplasty specimen. Organ cell cultures of normal and keratoconus corneal specimens were labeled with radioactive proline and analyzed by CM-cellulose chromatography and slab gel electrophoresis to determine collagen biosynthesis. Collagen types I and III were synthesized in similar amounts by normal and keratoconus stromacytes in culture. Specifically purified antibodies were used to determine the distribution of collagen types in tissue sections by immunofluorescence. The distribution of collagen types I, III, and IV in keratoconus was also similar to that in normal corneas, except that scarred regions in keratoconus and at the host-graft juncture were largely type III. Immunofluorescent reaction of the anti-type IV collagen antibodies with Bowman's layer, in particular, and Descemet's membrane in keratoconus specimens indicated extensive destruction. Basement membrane destruction may play an important role in this disease. [less ▲]

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See detailDetection of specific nitrated markers
Reginster, Jean-Yves ULg; DEBERG, Michelle ULg; Henrotin, Yves ULg et al

Patent (2011)

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See detailDetection of specific nitrated markers
REGINSTER, Jean-Yves ULg; DEBERG, Michelle ULg; Henrotin, Yves ULg et al

Patent (2012)

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See detailDETECTION OF SPECIFIC NITRATED MARKERS
Reginster, Jean-Yves ULg; Deberg, Michelle; Henrotin, Yves ULg et al

Patent (2004)

Methods are described for improving the diagnostic possibilities of diseases where oxidative NO-modifications occur, for example inflammatory conditions, cancer, Parkinson's or Alzheimer's disease, and to ... [more ▼]

Methods are described for improving the diagnostic possibilities of diseases where oxidative NO-modifications occur, for example inflammatory conditions, cancer, Parkinson's or Alzheimer's disease, and to provide means of monitoring the effects of therapeutical measures taken towards such diseases. The invention enables the detection of disease specific catabolic markers related to oxidative NO-modifications, utilizing an immunoassay comprising antibodies directed against nitrated and non-nitrated epitopes characteristic of a specific protein. [less ▲]

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See detailDetection of specific nitrated markers
REGINSTER, Jean-Yves ULg; DEBERG, Michelle ULg; Henrotin, Yves ULg et al

Patent (2008)

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See detailDetection of Staphylococcus aureus enterotoxins in meat using UPLC-MS/MS
Davin, Marie ULg

Master's dissertation (2013)

Detailed reference viewed: 45 (4 ULg)