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See detail25ans après, les Wallons sont-ils toujours belges ?
Italiano, Patrick ULg; Jacquemain, Marc ULg

in Cardelli, Rebecca; Bornand, Thierry; Brunet, Sébastien (Eds.) Baromètre social de la Wallonie. Engagement confiance, représentation et identité (2014)

Le chapitre parcourt la mesure des sentiments d'identité des wallons depuis 25 ans: les vagues d'enquête de 1988 à 2013 montrent les fluctuations des sentiments d'appartenance wallon, belge et européen en ... [more ▼]

Le chapitre parcourt la mesure des sentiments d'identité des wallons depuis 25 ans: les vagues d'enquête de 1988 à 2013 montrent les fluctuations des sentiments d'appartenance wallon, belge et européen en fonction sur le long terme, où apparaît clairement une déception par rapport à l'Europe après une euphorie initiale chez les jeunes, et qui ne se comble pas avec le temps. La donnée structurelle la plus stable est que les identités wallonne et belge sont complémentaires et non concurrentielles: à chaque enquête, cette corrélation est massive. Par contre, le sentiment d'être différent des Flamands a considérablement augmenté entre 2007 et 2013, ainsi que, sur la dernière période, le sentiment d'être différent de tous les "outgroups" qui augmente. [less ▲]

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See detail25KDa-Thiamine triphosphatase is essential for spermatozoid development in mice
Kohn, Grégory ULg; Ectors, Fabien ULg; Lakaye, Bernard ULg et al

Poster (2014, May)

For many years, our laboratory has been interested in thiamine triphosphate (ThTP), a vitamin B1 derivative whose metabolism and physiological role remain unclear. Regarding its production, we have shown ... [more ▼]

For many years, our laboratory has been interested in thiamine triphosphate (ThTP), a vitamin B1 derivative whose metabolism and physiological role remain unclear. Regarding its production, we have shown that in E. coli and in rat brain mitochondria, ThTP is synthesized from thiamine diphosphate and inorganic phosphate through a chemiosmotic mechanism involving the FoF1- ATP synthase [1, 2]. In mammalian cells, its concentration is maintained at a low level through hydrolysis by a very specific cytosolic 25-kDa thiamine triphosphatase (ThTPase) [3]. In order to gain insight in the role of ThTP and ThTPase in mammalian tissues, we decided to generate a mouse strain invalidated in 25kDa-ThTPase with the hope that these mice will accumulate ThTP in their tissues. We obtained genetically modified embryonic stem (ES) cells from the Knockout Mouse Project (KOMP) repository. In those cells, one of the 25kDa-ThTPase alleles was replaced by a construction containing the lacZ and the neomycin resistance genes. Those ES cells were microinjected in blastocysts and the chimeric blastocysts were injected in a mouse uterus to generate chimerae. However, when we bred those mice with wild type mice, the construction was never transmitted to the pups. To explain this result, we selected those chimerae that presented a sex-reversal. In those mice, all the spermatozoids derive from the injected embryonic stem cells, so that half of the spermatozoids are expected to harbor the construction. However, after qPCR analysis, we observed that the spermatozoids with the construction were outnumbered by a factor of thousand. These results strongly suggest that the 25kDa-ThTPase is required for spermatozoid development. 1. Gangolf, M., Wins, P., Thiry, M., El Moualij, B. & Bettendorff, L. (2010) J. Biol. Chem. 285, 583-94. 2. Gigliobianco, T., Gangolf, M., Lakaye, B., Pirson, B., von Ballmoos, C., Wins, P. & Bettendorff, L. (2013) Scientific reports. 3, 1071. 3. Lakaye, B., Makarchikov, A. F., Antunes, A. F., Zorzi, W., Coumans, B., De Pauw, E., Wins, P., Grisar, T. & Bettendorff, L. (2002) J. Biol. Chem. 277, 13771-7. [less ▲]

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See detail26th Annual Gospel Music Festival Chicago
Sacré, Robert ULg

Article for general public (2010)

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See detail26th Chicago Annual Gospel Music Festival
Sacré, Robert ULg

Article for general public (2010)

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See detail27 entrées du dictionnaire (Adorno, Bataille, Benjamin, Brecht, Burckhardt, etc.)
Hagelstein, Maud ULg

in Talon-Hugon, Carole (Ed.) Dictionnaire des théoriciens de l'art (in press)

Les notices du dictionnaire que je prends en charge : Adorno, Bataille, Benjamin, Burckhardt, Cassirer, Chastel, Deleuze, Dilthey, Faure, Focillon, Foucault, Francastel, Freud, Kracauer, Luckacs, Malraux ... [more ▼]

Les notices du dictionnaire que je prends en charge : Adorno, Bataille, Benjamin, Burckhardt, Cassirer, Chastel, Deleuze, Dilthey, Faure, Focillon, Foucault, Francastel, Freud, Kracauer, Luckacs, Malraux, Nietzsche, Panofsky, Riegl, Rousseau, Simmel, Valery, Vasari, Winkelman, Wölfflin, Worringer, Warburg. [less ▲]

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See detail27èmes Entretiens Jacques Cartier
Lambert, Marie ULg

Conference (2014, October 07)

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See detail28 milliards d’euros, c’est grand comment ?
Ozer, Pierre ULg; Salmon, Marc ULg; Theunissen, Yannick ULg

Article for general public (2009)

J’ouvre mon quotidien. Fortis : 28 milliards d’euros de pertes en 2008. Ma fille de sept ans qui commence à lire et pose sans cesse des questions sur tout me lance : « Dis-moi, papa : 28 milliards d’euros ... [more ▼]

J’ouvre mon quotidien. Fortis : 28 milliards d’euros de pertes en 2008. Ma fille de sept ans qui commence à lire et pose sans cesse des questions sur tout me lance : « Dis-moi, papa : 28 milliards d’euros, c’est grand comment ? ». Grande question… Je réfléchis et tente de trouver une parade parlante pour un enfant qui, in fine, se pose les mêmes questions que les grandes personnes. [less ▲]

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See detail286 jours ou le défi de la réconciliation du portrait et du récit
Delville, Michel ULg; Baetens, Jan

in Uhl, Magali (Ed.) Les récits visuels de soi (2015)

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See detail28th CHICAGO GOSPEL MUSIC FESTIVAL
Sacré, Robert ULg

in Blues & Rhythm - The Gospel Truth (2013), (282),

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See detailA 295 kDa intermediate filament-associated protein in radial glial and developing muscle cells in vivo and in vitro
Leprince, P; Chanas-Sacré, G; Thiry, Marc ULg et al

Poster (1999)

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See detailA 295-Kda Intermediate Filament-Associated Protein in Radial Glia and Developing Muscle Cells in Vivo and in Vitro
Chanas-Sacre, Grazyna; Thiry, Marc ULg; Pirard, Sandrine et al

in Developmental Dynamics : An Official Publication of the American Association of Anatomists (2000), 219(4), 514-25

The RC2 antibody is frequently used to label mouse radial glial cells in all parts of the nervous system where neuronal migration occurs during embryonic and early postnatal life. The antigen recognized ... [more ▼]

The RC2 antibody is frequently used to label mouse radial glial cells in all parts of the nervous system where neuronal migration occurs during embryonic and early postnatal life. The antigen recognized by this antibody still needs to be identified. We have characterized further its localization in vivo, its expression and subcellular localization in vitro, as well as its molecular nature. Histologic investigations of whole mouse embryos reveal an equally intense expression of RC2 immunostaining in radial glial cells in brain and spinal cord and in skeletal muscle. In glial cells cultures, the RC2 antibody recognizes an epitope located on the glial cytoskeleton and identified as an intermediate filament associated protein (IFAP) at the ultrastructural level. RC2 immunostaining in those cells is strongly dependent on the presence of a serum-derived activity. Serum-removal causes a decrease of the staining while adding serum back to the cells induces reexpression of RC2 immunoreactivity. By Western blotting, we find that in intermediate filament (IF) preparations obtained from cultured cerebellar glia, the RC2 antibody recognizes a 295-kDa protein whose expression is also dependent on the presence of serum in culture medium. In developing muscle cells, RC2 immunostaining is observed from the myoblast stage and disappears after complete myotube fusion. Both in vivo and in vitro, staining is first seen as a loose capping around myoblasts nuclei and progressively concentrates into Z-disks in association with the muscle IF protein desmin. The RC2 antibody also recognizes a 295-kDa protein band in muscle tissue protein extracts. Thus, the RC2 antibody recognizes a developmentally regulated cytoskeletal protein that is expressed, like other previously identified IFAPs, by cells of the glial and myogenic lineages and whose expression in vitro seems to be controlled by a signaling mechanism known to modulate astroglial morphology. [less ▲]

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See detail2B4 (CD244) is involved in eosinophil adhesion and chemotaxis, and its surface expression is increased in allergic rhinitis after challenge.
El-Shazly, Armel; HENKET, Monique ULg; Lefèbvre, Philippe ULg et al

in International Journal of Immunopathology and Pharmacology (2011), 24(4), 949-60

A role for the subtypes of CD2 Ig superfamily receptors has been recently demonstrated in eosinophilic inflammation in experimental asthma and atopic asthmatics. We investigated the functions of 2B4 ... [more ▼]

A role for the subtypes of CD2 Ig superfamily receptors has been recently demonstrated in eosinophilic inflammation in experimental asthma and atopic asthmatics. We investigated the functions of 2B4 (CD244) molecules in eosinophil adhesion and chemotaxis, and correlated the results to the pathophysiology of allergic rhinitis (AR). Herein, we show that agonistic stimulation of 2B4 by C1.7, the anti-human 2B4 functional grade purified antibody, resulted in significant increase of eosinophils and eosinophil cell line (Eol-1 cells) adhesion to collagen type IV, and random migration. These functions were associated with tyrosine kinase phosphorylation of several protein residues of low molecular weight. Flow cytometry (FACS) experiments demonstrated that Eol-1 cells, normal peripheral blood eosinophils and eosinophils from AR patients, express surface 2B4 molecules. In vitro AR model demonstrated that the CC-chemokine receptor CCR3 stimulation by eotaxin induced significant increase in the expression of surface 2B4 in eosinophils and Eol-1 cells. Immunofluorescence confocal microscopy images showed that eotaxin induces also redistribution of 2B4 molecules towards the pseudopods in eosinophils and Eol-1 cells, changing their shape. Blocking of 2B4 molecules by the corresponding neutralizing antibody inhibited eotaxin induced Eol-1-adhesion, chemotaxis and the cytoskeleton changes. Pretreatment of Eol-1 cells with 1 microM genistein blocked eotaxin-induced Eol-1 adhesion, chemotaxis and 2B4 up-regulated expression. In vivo correlation demonstrated the expression of 2B4 molecules in eosinophils from AR patients to be significantly increased, after nasal provocation challenge. These results identify a novel role for 2B4 molecules in eosinophil functional migratory response and may point to a novel tyrosine kinase-mediated ligation between CCR3 receptor and 2B4 co-receptor in eosinophil chemotaxis. If so, then 2B4 molecules would be a novel target for therapeutic modalities in diseases characterized by eosinophilia such as AR. [less ▲]

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See detail2D and 3D groundwater simulations to interpret tracer tests results in heterogeneous geological contexts
Dassargues, Alain ULg; Brouyère, Serge ULg; Carabin, Guy

in Kranjc, A. (Ed.) Tracer Hydrology 97 (1997)

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See detail2D AND 3D ROUTES TO INVESTIGATING PORES IN CONCRETE
Stroeven, Piet; He, Huan ULg; Le, L. B. N.

(2011, October)

Pores are of crucial engineering significance because they allow water and other harmful substances to enter the material. This paper reviews various methods for porosimetry that are applicable to ... [more ▼]

Pores are of crucial engineering significance because they allow water and other harmful substances to enter the material. This paper reviews various methods for porosimetry that are applicable to cementitious materials. Methods generally provide information on porosity and pore size distribution, yet pore continuity is required for transport, so should be investigated as well. Modern ways to approach such problems make use of computer facilities. Hence, the cementitious materials are produced in our case by a concurrent algorithm-based dynamic DEM system. Two novel methods that additionally render possible investigation of pore continuity are described in a separate paper on this conference [1] [less ▲]

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See detail2D cellular automata for turbulent convection
Perdang, Jean ULg; Raty, Jean-Yves ULg; Tomassini, M. et al

in Gruber, R.; Tomassini, M. (Eds.) Proceedings of the 6th Joint EPS-APS International Conference on Physics Computing. PC'94. Physics Computing '94 (1994)

A cellular automaton (CA) formulation is devised to simulate 2-dimensional Rayleigh-Benard convection. The model is tested in the case of a standard plane-parallel shallow configuration for which ... [more ▼]

A cellular automaton (CA) formulation is devised to simulate 2-dimensional Rayleigh-Benard convection. The model is tested in the case of a standard plane-parallel shallow configuration for which analytical solutions of the hydrodynamical formulation are available at low enough Rayleigh numbers. If we use a measure of the intensity of gravity, k, as our free parameter (the analogue of the Rayleigh number), all other model parameters being fixed, then we can reproduce the well-known stationary roll-pattern predicted by the hydrodynamic theory at low k values. At higher k values the model exhibits a nonstationary turbulent flow pattern. Our formulation is extended to less trivial geometries for which an analytic treatment in the usual hydrodynamic context is not feasible. Besides geometry-constrained stationary patterns of motion in a range of low k values, this model exhibits again turbulent motions at higher k values [less ▲]

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See detail2D Computations of g-modes in Fast Rotating Stars
Ballot, J.; Lignières, F.; Prat, V. et al

in Shibahashi, H; Takata, M; Lynas-Gray, A. E. (Eds.) Progress in Solar/Stellar Physics with Helio- and Asteroseismology (2012, September 01)

We present complete 2D computations of g-modes in distorted polytropic models of stars performed with the Two-dimensional Oscillation Program (TOP). We computed low-degree modes (ℓ =1 modes with radial ... [more ▼]

We present complete 2D computations of g-modes in distorted polytropic models of stars performed with the Two-dimensional Oscillation Program (TOP). We computed low-degree modes (ℓ =1 modes with radial order n = -1,…,-14, and ℓ = 2, 3 modes with n = -1,…,-5 and -16,…,-20) of a non-rotating model and followed them by slowly increasing the rotation rate up to 70 % of the Keplerian break-up velocity. We use these computations to determine the domain of validity of perturbative methods up to the 3rd order. We study the evolution of the regularities of the spectrum and show quantitative agreement with the traditional approximation for not too large values of the ratio of the rotation rate to the pulsation frequency. We also show the appearance of new types of modes, called “rosette” modes due to their spatial structure. Thanks to the ray theory for gravito-inertial waves that we developed, we can associate these modes with stable periodic rays. [less ▲]

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See detail2D DIGE, label free quantification, principal component and mass spectrometry analysis for biomarkers discovery in MCF-7/BOS cells exposed to 17β-estradiol and endocrine disruptors.
Collodoro, Mike ULg; Lemaire, Pascale; Eppe, Gauthier ULg et al

in Organohalogen Compounds (2011)

Endocrine system disruption has become a subject of great interest over the last few decades, since it has become evident that natural and also synthetic substances can mimic or reduce the activity of ... [more ▼]

Endocrine system disruption has become a subject of great interest over the last few decades, since it has become evident that natural and also synthetic substances can mimic or reduce the activity of endogenous hormones. Compounds with estrogenic activity are an important family of potential endocrine disruptors that have to be monitored either in the food chain or in the environment. Estrogens are known to induce or promote hormonal dependent cancers, to reduce sperm counts and fertility in men and generate the feminization of exposed wildlife populations. The rapid screening of unwanted chemicals in the food chain is beset by difficulties. The number of toxic compounds is very large and no universal method can cope with their diversity. In this work, emergent differential proteomic techniques are used to discover a set of biomarkers for the development of a multiple estrogen contaminants screening test. [less ▲]

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