Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detail2F3 Monoclonal Antibody Recognizes the O26 O-Antigen Moiety of the Lipopolysaccharide of Enterohemorrhagic Escherichia coli Strain 4276
Szalo, Ioan Mihai ULg; Taminiau, Bernard ULg; Goffaux, Frédéric et al

in Clinical and Diagnostic Laboratory Immunology (2004), 11(3), 532-537

Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) organisms are groups of pathogenic strains whose infections are characterized by a typical lesion of enterocyte attachment and ... [more ▼]

Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) organisms are groups of pathogenic strains whose infections are characterized by a typical lesion of enterocyte attachment and effacement. They are involved in enteric diseases both in humans and in animals, and EHEC strains can be responsible for hemolytic uremic syndrome in humans. Previously, it was shown that the 2F3 monoclonal antibody (MAb) is specific for the O26 EHEC and EPEC strains (P. Kerr, H. Ball, B. China, J. Mainil, D. Finlay, D. Pollock, I. Wilson, and D. Mackie, Clin. Diagn. Lab. Immunol. 6:610–614, 1999). As these groups of bacteria play an important role in pathology, the aim of this paper was to characterize the antigen recognized by the 2F3 MAb and its genetic determinant. A genomic locus containing the entire O-antigen gene cluster and half of the colanic acid gene cluster from an O26 EHEC strain was shown to be sufficient for the production of the antigen recognized by the 2F3 MAb in an E. coli DH5 strain. By transposon mutagenesis performed on the recombinant plasmid, all 2F3 enzyme-linked immunosorbent assay-negative mutants had their transposons inserted into the O-antigen gene cluster. The O-antigen gene cluster was also cloned from an O26 EHEC strain into the E. coli DH5 strain, which then produced a positive result with the 2F3 MAb. Further analysis of the type of lipopolysaccharides (smooth or rough) produced by the clones and mutants and of the O antigen of the 2F3-positive clones confirmed that the epitope recognized by the 2F3 MAb is located on the O antigen in the O26 EHEC and EPEC strains and that its genetic determinant is located inside the O-antigen gene cluster. [less ▲]

Detailed reference viewed: 51 (8 ULg)
Full Text
See detailThe 2nd Workshop on Active Internet Measurements (AIMS-2) Report
claffy, kc; Aben, Emile; Auge, Jorge, et al

in Computer Communication Review (2010), 40(5), 53-58

On February 8-10, 2010, CAIDA hosted the second Work- shop on Active Internet Measurements (AIMS-2) as part of our series of Internet Statistics and Metrics Analysis (ISMA) workshops. The goals of this ... [more ▼]

On February 8-10, 2010, CAIDA hosted the second Work- shop on Active Internet Measurements (AIMS-2) as part of our series of Internet Statistics and Metrics Analysis (ISMA) workshops. The goals of this workshop were to further our understanding of the potential and limitations of active mea- surement research and infrastructure in the wide-area Inter- net, and to promote cooperative solutions and coordinated strategies to addressing future data needs of the network and security research communities. The three-day workshop included presentations, group discussion and analysis, and focused interaction between participating researchers, oper- ators, and policymakers from all over the world. This report describes the motivation and findings of the workshop, and reviews progress on recommendations developed at the 1st Active Internet Measurements Workshop in 2009 [18]. Slides from the workshop presentations are available at [9]. [less ▲]

Detailed reference viewed: 8 (1 ULg)
Full Text
Peer Reviewed
See detail3 ',4 ',5 ',6 ' tetradehydrolongicaudatine Y, an anhydronium base from Strychnos usambarensis
Frederich, Michel ULg; Quetin-Leclercq, J.; Biala, R. G. et al

in Phytochemistry (1998), 48(7), 1263-1266

Detailed reference viewed: 19 (5 ULg)
Full Text
Peer Reviewed
See detailThe 3' region of bovine leukemia virus genome encodes a trans-activator protein.
Rosen, C. A.; Sodroski, J. G.; Willems, Luc ULg et al

in EMBO Journal (1986), 5(10), 2585-9

The genome of bovine leukemia virus (BLV) contains several overlapping, long open reading frames 3' to the envelope gene. Experiments presented here show that the cDNA encompassing the X region open ... [more ▼]

The genome of bovine leukemia virus (BLV) contains several overlapping, long open reading frames 3' to the envelope gene. Experiments presented here show that the cDNA encompassing the X region open reading frames encodes a trans-activator function capable of increasing the level of gene expression directed by the BLV long terminal repeat sequences. This study provides further evidence of the structural and functional similarities of the bovine leukemia virus and the human T lymphotropic viruses, HTLV-I and HTLV-II. [less ▲]

Detailed reference viewed: 11 (3 ULg)
Full Text
Peer Reviewed
See detailLa 3'-deoxy-3'-[18F] fluorothymidine ([18F]-FLT) est-elle le prochain traceur utilise en routine pour la TEP apres le [18F]-FDG?
Couturier, Olivier; Leost, Francoise; Campone, Mario et al

in Bulletin du Cancer (2005), 92(9), 789-98

Positron emission tomography (PET) with [18F]-FDG is now firmly established as a clinical tool in oncology. Its applications are however limited in some indications, due to the lack of specificity of its ... [more ▼]

Positron emission tomography (PET) with [18F]-FDG is now firmly established as a clinical tool in oncology. Its applications are however limited in some indications, due to the lack of specificity of its uptake mechanism for tumors, or the low avidity of some cancer types such as prostate. Alternative tracers are thus being developed, in order to fill up this void. Proliferation as a biological target is particularly attractive in cancer imaging. From that perspective, fluorothymidine ([18F]-FLT or FLT) has generated a strong interest among the scientific community, especially since the radiosynthesis process has been improved and simplified, thus making possible to envision a routine use for the tracer. This article aims at summarizing the status of the current scientific data regarding FLT. The uptake mechanism of FLT is well known, relying on the thymidine kinase 1 (TK1) enzymatic activity, and thus on DNA synthesis. Preclinical studies have shown a clear relationship between tracer accumulation and level of tumor proliferation, even though DNA salvage pathwayss intervene in the process and may complicate the interpretation of the results. Several clinical studies suggest a good specificity for tumor, albeit with a lower sensitivity than with FDG. In all likelihood however, the future of FLT lies in the evaluation of antitumor response and possibly the pretherapeutic prognostic characterization, rather than in the diagnosis and staging of malignancies. Although the scientific data regarding this issue remain limited, initial results are encouraging. Further significant work remains to be done in order to fully assess the clinical performances of the tracer, on the one hand, and to determine its place relative to FDG and other emerging tracers, on the other hand. Until these studies are completed, FLT should be considered as a promising tracer, but remaining at an experimental stage of its development. [less ▲]

Detailed reference viewed: 53 (1 ULg)
Full Text
Peer Reviewed
See detail3,6-Disubstitued coumarins as mechanism-based inhibitors of thrombin and factor Xa
Frederick, R.; Robert, S.; Charlier, C. et al

in Journal of Medicinal Chemistry (2005), 48

Detailed reference viewed: 10 (4 ULg)
Full Text
Peer Reviewed
See detail3,7-Dimethyl-4H-pyrido[4,3-e]-1,2,4-thiadiazine 1,1-dioxide zwitterion
Dupont, L.; Pirotte, Bernard ULg; De Tullio, Pascal ULg et al

in Acta Crystallographica (1995), C51

Detailed reference viewed: 4 (1 ULg)
Peer Reviewed
See detailLes 3-alkylamino-2-méthyl-2H-pyrido[4,3-e]-1,2,4-thiadiazine 1,1-dioxydes en tant que nouveaux agents myorelâchants
Pirotte, Bernard ULg; Ouedraogo, R.; Fang, Z. Y. et al

Poster (1996, January 12)

Detailed reference viewed: 3 (0 ULg)