Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
See detailA 295 kDa intermediate filament-associated protein in radial glial and developing muscle cells in vivo and in vitro
Leprince, P; Chanas-Sacré, G; Thiry, Marc ULg et al

Poster (1999)

Detailed reference viewed: 4 (0 ULg)
Full Text
Peer Reviewed
See detailA 295-Kda Intermediate Filament-Associated Protein in Radial Glia and Developing Muscle Cells in Vivo and in Vitro
Chanas-Sacre, Grazyna; Thiry, Marc ULg; Pirard, Sandrine et al

in Developmental Dynamics : An Official Publication of the American Association of Anatomists (2000), 219(4), 514-25

The RC2 antibody is frequently used to label mouse radial glial cells in all parts of the nervous system where neuronal migration occurs during embryonic and early postnatal life. The antigen recognized ... [more ▼]

The RC2 antibody is frequently used to label mouse radial glial cells in all parts of the nervous system where neuronal migration occurs during embryonic and early postnatal life. The antigen recognized by this antibody still needs to be identified. We have characterized further its localization in vivo, its expression and subcellular localization in vitro, as well as its molecular nature. Histologic investigations of whole mouse embryos reveal an equally intense expression of RC2 immunostaining in radial glial cells in brain and spinal cord and in skeletal muscle. In glial cells cultures, the RC2 antibody recognizes an epitope located on the glial cytoskeleton and identified as an intermediate filament associated protein (IFAP) at the ultrastructural level. RC2 immunostaining in those cells is strongly dependent on the presence of a serum-derived activity. Serum-removal causes a decrease of the staining while adding serum back to the cells induces reexpression of RC2 immunoreactivity. By Western blotting, we find that in intermediate filament (IF) preparations obtained from cultured cerebellar glia, the RC2 antibody recognizes a 295-kDa protein whose expression is also dependent on the presence of serum in culture medium. In developing muscle cells, RC2 immunostaining is observed from the myoblast stage and disappears after complete myotube fusion. Both in vivo and in vitro, staining is first seen as a loose capping around myoblasts nuclei and progressively concentrates into Z-disks in association with the muscle IF protein desmin. The RC2 antibody also recognizes a 295-kDa protein band in muscle tissue protein extracts. Thus, the RC2 antibody recognizes a developmentally regulated cytoskeletal protein that is expressed, like other previously identified IFAPs, by cells of the glial and myogenic lineages and whose expression in vitro seems to be controlled by a signaling mechanism known to modulate astroglial morphology. [less ▲]

Detailed reference viewed: 75 (4 ULg)
Full Text
Peer Reviewed
See detail2B4 (CD244) is involved in eosinophil adhesion and chemotaxis, and its surface expression is increased in allergic rhinitis after challenge.
El-Shazly, Armel; HENKET, Monique ULg; Lefèbvre, Philippe ULg et al

in International Journal of Immunopathology and Pharmacology (2011), 24(4), 949-60

A role for the subtypes of CD2 Ig superfamily receptors has been recently demonstrated in eosinophilic inflammation in experimental asthma and atopic asthmatics. We investigated the functions of 2B4 ... [more ▼]

A role for the subtypes of CD2 Ig superfamily receptors has been recently demonstrated in eosinophilic inflammation in experimental asthma and atopic asthmatics. We investigated the functions of 2B4 (CD244) molecules in eosinophil adhesion and chemotaxis, and correlated the results to the pathophysiology of allergic rhinitis (AR). Herein, we show that agonistic stimulation of 2B4 by C1.7, the anti-human 2B4 functional grade purified antibody, resulted in significant increase of eosinophils and eosinophil cell line (Eol-1 cells) adhesion to collagen type IV, and random migration. These functions were associated with tyrosine kinase phosphorylation of several protein residues of low molecular weight. Flow cytometry (FACS) experiments demonstrated that Eol-1 cells, normal peripheral blood eosinophils and eosinophils from AR patients, express surface 2B4 molecules. In vitro AR model demonstrated that the CC-chemokine receptor CCR3 stimulation by eotaxin induced significant increase in the expression of surface 2B4 in eosinophils and Eol-1 cells. Immunofluorescence confocal microscopy images showed that eotaxin induces also redistribution of 2B4 molecules towards the pseudopods in eosinophils and Eol-1 cells, changing their shape. Blocking of 2B4 molecules by the corresponding neutralizing antibody inhibited eotaxin induced Eol-1-adhesion, chemotaxis and the cytoskeleton changes. Pretreatment of Eol-1 cells with 1 microM genistein blocked eotaxin-induced Eol-1 adhesion, chemotaxis and 2B4 up-regulated expression. In vivo correlation demonstrated the expression of 2B4 molecules in eosinophils from AR patients to be significantly increased, after nasal provocation challenge. These results identify a novel role for 2B4 molecules in eosinophil functional migratory response and may point to a novel tyrosine kinase-mediated ligation between CCR3 receptor and 2B4 co-receptor in eosinophil chemotaxis. If so, then 2B4 molecules would be a novel target for therapeutic modalities in diseases characterized by eosinophilia such as AR. [less ▲]

Detailed reference viewed: 21 (4 ULg)
Full Text
See detail2D and 3D groundwater simulations to interpret tracer tests results in heterogeneous geological contexts
Dassargues, Alain ULg; Brouyère, Serge ULg; Carabin, Guy

in Kranjc, A. (Ed.) Tracer Hydrology 97 (1997)

Detailed reference viewed: 46 (10 ULg)
Full Text
Peer Reviewed
See detail2D AND 3D ROUTES TO INVESTIGATING PORES IN CONCRETE
Stroeven, Piet; He, Huan ULg; Le, L. B. N.

(2011, October)

Pores are of crucial engineering significance because they allow water and other harmful substances to enter the material. This paper reviews various methods for porosimetry that are applicable to ... [more ▼]

Pores are of crucial engineering significance because they allow water and other harmful substances to enter the material. This paper reviews various methods for porosimetry that are applicable to cementitious materials. Methods generally provide information on porosity and pore size distribution, yet pore continuity is required for transport, so should be investigated as well. Modern ways to approach such problems make use of computer facilities. Hence, the cementitious materials are produced in our case by a concurrent algorithm-based dynamic DEM system. Two novel methods that additionally render possible investigation of pore continuity are described in a separate paper on this conference [1] [less ▲]

Detailed reference viewed: 8 (2 ULg)
See detail2D cellular automata for turbulent convection
Perdang, Jean ULg; Raty, Jean-Yves ULg; Tomassini, M. et al

in Gruber, R.; Tomassini, M. (Eds.) Proceedings of the 6th Joint EPS-APS International Conference on Physics Computing. PC'94. Physics Computing '94 (1994)

A cellular automaton (CA) formulation is devised to simulate 2-dimensional Rayleigh-Benard convection. The model is tested in the case of a standard plane-parallel shallow configuration for which ... [more ▼]

A cellular automaton (CA) formulation is devised to simulate 2-dimensional Rayleigh-Benard convection. The model is tested in the case of a standard plane-parallel shallow configuration for which analytical solutions of the hydrodynamical formulation are available at low enough Rayleigh numbers. If we use a measure of the intensity of gravity, k, as our free parameter (the analogue of the Rayleigh number), all other model parameters being fixed, then we can reproduce the well-known stationary roll-pattern predicted by the hydrodynamic theory at low k values. At higher k values the model exhibits a nonstationary turbulent flow pattern. Our formulation is extended to less trivial geometries for which an analytic treatment in the usual hydrodynamic context is not feasible. Besides geometry-constrained stationary patterns of motion in a range of low k values, this model exhibits again turbulent motions at higher k values [less ▲]

Detailed reference viewed: 53 (0 ULg)
Full Text
See detail2D Computations of g-modes in Fast Rotating Stars
Ballot, J.; Lignières, F.; Prat, V. et al

in Shibahashi, H; Takata, M; Lynas-Gray, A. E. (Eds.) Progress in Solar/Stellar Physics with Helio- and Asteroseismology (2012, September 01)

We present complete 2D computations of g-modes in distorted polytropic models of stars performed with the Two-dimensional Oscillation Program (TOP). We computed low-degree modes (ℓ =1 modes with radial ... [more ▼]

We present complete 2D computations of g-modes in distorted polytropic models of stars performed with the Two-dimensional Oscillation Program (TOP). We computed low-degree modes (ℓ =1 modes with radial order n = -1,…,-14, and ℓ = 2, 3 modes with n = -1,…,-5 and -16,…,-20) of a non-rotating model and followed them by slowly increasing the rotation rate up to 70 % of the Keplerian break-up velocity. We use these computations to determine the domain of validity of perturbative methods up to the 3rd order. We study the evolution of the regularities of the spectrum and show quantitative agreement with the traditional approximation for not too large values of the ratio of the rotation rate to the pulsation frequency. We also show the appearance of new types of modes, called “rosette” modes due to their spatial structure. Thanks to the ray theory for gravito-inertial waves that we developed, we can associate these modes with stable periodic rays. [less ▲]

Detailed reference viewed: 18 (7 ULg)
Full Text
Peer Reviewed
See detail2D DIGE, label free quantification, principal component and mass spectrometry analysis for biomarkers discovery in MCF-7/BOS cells exposed to 17β-estradiol and endocrine disruptors.
Collodoro, Mike ULg; Lemaire, Pascale; Eppe, Gauthier ULg et al

in Organohalogen Compounds (2011)

Endocrine system disruption has become a subject of great interest over the last few decades, since it has become evident that natural and also synthetic substances can mimic or reduce the activity of ... [more ▼]

Endocrine system disruption has become a subject of great interest over the last few decades, since it has become evident that natural and also synthetic substances can mimic or reduce the activity of endogenous hormones. Compounds with estrogenic activity are an important family of potential endocrine disruptors that have to be monitored either in the food chain or in the environment. Estrogens are known to induce or promote hormonal dependent cancers, to reduce sperm counts and fertility in men and generate the feminization of exposed wildlife populations. The rapid screening of unwanted chemicals in the food chain is beset by difficulties. The number of toxic compounds is very large and no universal method can cope with their diversity. In this work, emergent differential proteomic techniques are used to discover a set of biomarkers for the development of a multiple estrogen contaminants screening test. [less ▲]

Detailed reference viewed: 35 (3 ULg)
Full Text
Peer Reviewed
See detail2D electrostatic problems with rounded corners
Buret, François; Dauge, Monique; Dular, Patrick ULg et al

in 18th Conference on the Computation of Electromagnetic Fields (COMPUMAG2011) (2011, July)

The second order terms of a multiscale expansion for dealing with rounded corners in 2D electrostatic problems are studied. The heuristics and the sequence of problems to be solved are presented and ... [more ▼]

The second order terms of a multiscale expansion for dealing with rounded corners in 2D electrostatic problems are studied. The heuristics and the sequence of problems to be solved are presented and finite element simulations demonstrate the accuracy of the method. [less ▲]

Detailed reference viewed: 13 (0 ULg)
Full Text
Peer Reviewed
See detailA 2D finite volume multiblock flow solver applied to flood extension forecasting
Erpicum, Sébastien ULg; Archambeau, Pierre ULg; Detrembleur, Sylvain ULg et al

in García-Navarro, Pilar; Playán, Enrique (Eds.) Numerical modelling of hydrodynamics for water ressources (2008)

ABSTRACT: this paper describes a quasi-3D free surface flow solver,developed for almost ten years at the University of Liege, and its application to flood extension forecasting. Based on the classical SWE ... [more ▼]

ABSTRACT: this paper describes a quasi-3D free surface flow solver,developed for almost ten years at the University of Liege, and its application to flood extension forecasting. Based on the classical SWE, the finite volume numerical model deals with real topography and wetting-drying cells while exactly conserving the water volume. Multiblock options allow local refine-ment of the structured calculation grid and automatic mesh refinement enhances greatly compu-tation time. An additional depth-averaged k-ε type turbulence model increases the potentialities of the solver to represent real complex flows. Following a Regional Government decision, this solver has been officially chosen in 2003 to be used to compute inundation maps on 800 km of the main rivers of the Walloon Region in Belgium with a spatial resolution of 5 x 5 to 2 x 2 me-ters and using high resolution Digital Elevation Models. Results are presented in this paper. [less ▲]

Detailed reference viewed: 33 (1 ULg)
Full Text
Peer Reviewed
See detail2D Harmonic Balance FE Modelling of Electromagnetic Devices coupled to Nonlinear Circuits
Gyselinck, J.; Dular, Patrick ULg; Geuzaine, Christophe ULg et al

in COMPEL (2004), 23(3), 800--812

Detailed reference viewed: 32 (6 ULg)
Full Text
See detail2D non-perturbative modeling of oscillations in rapidly rotating stars
Ouazzani, Rhita-Maria ULg; Dupret, Marc-Antoine ULg; Goupil, M. J. et al

in Astronomical Notes (2010), 331

We present and discuss results of a recently developped two dimensional non-perturbative method to compute accurate adiabatic oscillation modes of rapidly rotating stars . The 2D calculations fully take ... [more ▼]

We present and discuss results of a recently developped two dimensional non-perturbative method to compute accurate adiabatic oscillation modes of rapidly rotating stars . The 2D calculations fully take into account the centrifugal distorsion of the star while the non-perturbative method includes the full influence of the Coriolis acceleration. These characteristics allows us to compute oscillation modes of rapid rotators - from high order p-modes in $\delta$Scuti stars, to low order p- and g-modes in $\beta$ Cephei or Be stars. [less ▲]

Detailed reference viewed: 4 (0 ULg)
Full Text
Peer Reviewed
See detail2D numerical flow modeling in a macro-rough channel
Erpicum, Sébastien ULg; Meile, Tobias; Dewals, Benjamin ULg et al

in International Journal for Numerical Methods in Fluids (2009), 61(11), 1227-1246

A 2D numerical flow model, developed at the Research unit of Hydrology, Applied Hydrodynamics and Hydraulic Constructions at ULg, has been applied to flows in a macro-rough channel. The model solves the ... [more ▼]

A 2D numerical flow model, developed at the Research unit of Hydrology, Applied Hydrodynamics and Hydraulic Constructions at ULg, has been applied to flows in a macro-rough channel. The model solves the shallow water equations (SWE) with a two length scale, depth-integrated k-type approach for turbulence modeling. Data for the comparison have been provided by experiments conducted at the Laboratory of Hydraulic Constructions at EPFL. In the experiments with different non-prismatic channel configurations, namely large-scale cavities at the side walls, three different 2D flow characteristics could be observed in cavities. With the used numerical model features, especially regarding turbulence and friction modeling, a single set of bottom and side wall roughness could be found for a large range of discharges investigated in a prismatic channel. For the macro rough configurations, the numerical model gives an excellent agreement between experimental and numerical results regarding backwater curves and flow patterns if the side wall cavities have low aspect ratios. For configurations with high aspect ratios, the head loss generated by the preservation of important recirculation gyres in the cavities is slightly underestimated. The results of the computations reveal clearly that the separation of turbulence sources in the mathematical model is of great importance. Indeed, the turbulence related to 2D transverse shear effects and the 3D turbulence, generated by bed friction, can have very different amplitude. When separating these two effects in the numerical models, most of the flow features observed experimentally can be reproduced accurately. [less ▲]

Detailed reference viewed: 175 (66 ULg)
Full Text
Peer Reviewed
See detail2D to 3D crossover of the magnetic properties in ordered arrays of iron oxide nanocrystals
Faure, Bertrand; Wetterskog, Erik; Gunnarsson, Klas et al

in NANOSCALE (2013), 5(3), 953-960

The magnetic 2D to 3D crossover behavior of well-ordered arrays of monodomain gamma-Fe2O3 spherical nanoparticles with different thicknesses has been investigated by magnetometry and Monte Carlo (MC ... [more ▼]

The magnetic 2D to 3D crossover behavior of well-ordered arrays of monodomain gamma-Fe2O3 spherical nanoparticles with different thicknesses has been investigated by magnetometry and Monte Carlo (MC) simulations. Using the structural information of the arrays obtained from grazing incidence small-angle X-ray scattering and scanning electron microscopy together with the experimentally determined values for the saturation magnetization and magnetic anisotropy of the nanoparticles, we show that MC simulations can reproduce the thickness-dependent magnetic behavior. The magnetic dipolar particle interactions induce a ferromagnetic coupling that increases in strength with decreasing thickness of the array. The 2D to 3D transition in the magnetic properties is mainly driven by a change in the orientation of the magnetic vortex states with increasing thickness, becoming more isotropic as the thickness of the array increases. Magnetic anisotropy prevents long-range ferromagnetic order from being established at low temperature and the nanoparticle magnetic moments instead freeze along directions defined by the distribution of easy magnetization directions. [less ▲]

Detailed reference viewed: 33 (0 ULg)
Full Text
Peer Reviewed
See detailA 2D vertical finite volume solver using a level set approach for simulating free surface incompressible flows
Detrembleur, Sylvain ULg; Dewals, Benjamin ULg; Erpicum, Sébastien ULg et al

in European Journal of Mechanical and Environmental Engineering (2009), 2009(3), 4-9

Detailed reference viewed: 64 (20 ULg)
Full Text
Peer Reviewed
See detail2D XFEM-based modeling of retraction and successive resections for preoperative image update.
Vigneron, Lara M.; Duflot, Marc P.; Robe, Pierre ULg et al

in Computer aided surgery : official journal of the International Society for Computer Aided Surgery (2009), 14(1-3), 1-20

This paper considers an approach to improving outcomes for neurosurgery patients by enhancing intraoperative navigation and guidance. Currently, intraoperative navigation systems do not accurately account ... [more ▼]

This paper considers an approach to improving outcomes for neurosurgery patients by enhancing intraoperative navigation and guidance. Currently, intraoperative navigation systems do not accurately account for brain shift or tissue resection. We describe how preoperative images can be incrementally updated to take into account any type of brain tissue deformation that may occur during surgery, and thus to improve the accuracy of image-guided navigation systems. For this purpose, we have developed a non-rigid image registration technique using a biomechanical model, which deforms based on the Finite Element Method (FEM). While the FEM has been used successfully for dealing with deformations such as brain shift, it has difficulty with tissue discontinuities. Here, we describe a novel application of the eXtended Finite Element Method (XFEM) in the field of image-guided surgery in order to model brain deformations that imply tissue discontinuities. In particular, this paper presents a detailed account of the use of XFEM for dealing with retraction and successive resections, and demonstrates the feasibility of the approach by considering 2D examples based on intraoperative MR images. To evaluate our results, we compute the modified Hausdorff distance between Canny edges extracted from images before and after registration. We show that this distance decreases after registration, and thus demonstrate that our approach improves alignment of intraoperative images. [less ▲]

Detailed reference viewed: 15 (0 ULg)
Full Text
Peer Reviewed
See detail2D-Vertical Model for Free Surface Incompressible Flows: Validation and Applications
Detrembleur, Sylvain; Dewals, Benjamin ULg; Pirotton, Michel ULg

in Proc. 34th IAHR World Congress - Balance and Uncertainty (2011)

Efficient design of hydraulic structures such as weirs and bottom outlets requires a detailed description of the complex turbulent flows occurring on these structures. Compared to laboratory experiments ... [more ▼]

Efficient design of hydraulic structures such as weirs and bottom outlets requires a detailed description of the complex turbulent flows occurring on these structures. Compared to laboratory experiments, numerical modeling offers more flexibility and cost-effectiveness to test different designs. The non-hydrostatic 2D-vertical model presented here solves the RANS equations for incompressible flows in the vertical plane. It is based on the projection method for pressure-velocity coupling, while free surface tracking is conducted by means of the level set technique and Sussman’s velocity extension algorithm. It enables to predict accurately the flow pattern in the vertical plane and deals with multiple interfaces (jets, drops…). The solver has been validated for both steady and unsteady free surface flows, based on theoretical and experimental data. The paper will detail the application of the model to a pressurized flow and to a sloshing tank. [less ▲]

Detailed reference viewed: 32 (7 ULg)