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See detailDefinition of quality standards applicable to the purification and the expansion of mesenchymal stem cells for their use in haematopoietic, immunosuppressive and regenerative cellular therapy
Briquet, Alexandra ULg

Master of advanced studies dissertation (2005)

Mesenchymal stem cells (MSCs) reside within the stromal compartment of bone marrow and represent a plastic cell population for which pro-haematopoietic, immunosuppressive and regenerative properties are ... [more ▼]

Mesenchymal stem cells (MSCs) reside within the stromal compartment of bone marrow and represent a plastic cell population for which pro-haematopoietic, immunosuppressive and regenerative properties are poorly characterized. A characterization of MSC preparations was carried out with each passage in MSCGM or α-MEM + 20% FBS expansion media. We obtained 3 times more cells after 5 passages in MSCGM than in α-MEM + 20% FBS. We analysed the phenotype of MSCs after each passage by flow cytometry. At all passages and in both media cells were tested negative for CD45, strongly positive for CD73 and CD90 antigens and weakly positive for CD105 and CD106 antigens. We wanted to assess the MSC ability to differentiate into adipocytes, osteoblasts and chondroblasts. At all tested passages and in both media, MSCs differentiated in these three tissues when they were placed in the adequate induction medium. We used RayBio® Human Cytokine Antibody Array to analyse the presence of several cytokines in MSC-conditioned medium. At all passages and in both media, IL-6, IL-8 and VCAM-1 were more strongly expressed than the other cytokines. We used the CFU-F assay to evaluate the frequency and purity of MSCs in culture. We observed that CFU-F number reached a maximum at passages 3 and 4 after expansion in both MSCGM and in α-MEM + 20% FBS. We also observed a greater proportion of CFU-F for the cells expanded in MSCGM compared to the cells expanded in α-MEM + 20% FBS. To assess the haematopoietic supporting ability of MSCs, long term cultures were performed. We observed more colony-forming cells when long-term cultures were done with 1st and 2nd passages MSCs than when cultures were done with 3rd, 4th and 5th passages MSCs. In NOD/SCID mice we transplanted CD34+ uncultured cells or the expansion product of CD34+ cells co-cultured for one week with MSCs. Human chimerism was present in all conditions. We observed a greater human chimerism when CD34+ cells were co-cultured with MSCs from passage 4 than in the other conditions. In all conditions simultaneous expression of CD19 or CD33 antigens on human CD45+ cells demonstrated the presence of repopulating cells with lympho-myeloid potential. Despite the fact that MSCs had the same phenotype, differentiation potential and cytokine secretion profile at each passage and in both media, MSCs did have neither the same capacity to form CFU-F nor the same capacity to support haematopoiesis in vitro and in vivo. [less ▲]

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See detailDefinition of vocal pitch accuracy in a melodic context
Larrouy, Pauline ULg

Scientific conference (2014, March 28)

If we easily categorize sung performances as “in tune” or “out of tune”, it is not so evident to explicitly describe on which criteria our evaluation is based. This presentation reports recent work on ... [more ▼]

If we easily categorize sung performances as “in tune” or “out of tune”, it is not so evident to explicitly describe on which criteria our evaluation is based. This presentation reports recent work on pitch accuracy perception in a melodic context. The results allow to refine the notion of pitch accuracy and therefore to design objective tools for the evaluation of singer quality. [less ▲]

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See detailUne définition opérationnelle de la Métacognition et ses mises en oeuvre
Leclercq, Dieudonné ULg; Poumay, Marianne ULg

in 21e colloque de l'Association Internationale de Pédagogie Universitaire (A.I.P.U.) Université Cadi Ayyad de Marrakech (Maroc). "L'AIPU : 20 ans de recherches et d'actions pédagogiques; bilan et perspectives." (2004)

Detailed reference viewed: 266 (28 ULg)
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See detailLa définition platonicienne de la vertu
Gavray, Marc-Antoine ULg

in Annuaire de l'École Pratique des Hautes Etudes. Section des Sciences Religieuses (2012), 119(2010-2011), 149-155

Detailed reference viewed: 41 (3 ULg)
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See detailDéfinition, notions de base, raison d'être et sources juridiques des procédures de participation du public
Delnoy, Michel ULg

in Jadot, Benoït (Ed.) La participation du public au processus de décision en matière d'environnement et d'urbanisme (2005)

Detailed reference viewed: 249 (14 ULg)
See detailDéfinitions fondamentales d’une carène et analyse de sa stabilité
Marchal, Jean ULg

Learning material (1995)

Detailed reference viewed: 16 (4 ULg)
See detailDefinizioni, Indefinibili e Indefiniti; una questione semiotica
Cigana, Lorenzo ULg

Scientific conference (2013, December 09)

Detailed reference viewed: 9 (0 ULg)
See detailDéfis à l'économie rurale : livre commémoratif du 20ème anniversaire de l'Association Belge d'Economie Rurale
Burny, Philippe ULg; Mathijs, E.; Mottoulle, A. et al

Book published by Garant (1999)

Detailed reference viewed: 12 (1 ULg)
See detailDéfis alimentaires et climatiques: regards croisés.
Kabamba, Bob ULg

Scientific conference (2009)

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See detailLes défis colossaux auxquels la santé publique va devoir faire face !
COUCKE, Philippe ULg

in Onco : Revue Multidisciplinaire d'Oncologie (2014)

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See detailLes défis d'une formation universitaire clinique en logopédie
Schelstraete, Marie-Anne; Maillart, Christelle ULg

in Estienne, Françoise; Vanderlinden, Fabienne (Eds.) Pratiquer l'orthophonie. Expériences et savoir-faire de 33 orthophonistes. (2012)

Detailed reference viewed: 215 (23 ULg)
See detailLes défis de l'agroalimentaire: exemple de l'huile de palme
Delacharlerie, Sophie ULg; Chèné, Christine; Sindic, Marianne ULg

Scientific conference (2012, June 26)

Detailed reference viewed: 59 (14 ULg)
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See detailLes défis de l'étalement urbain : clarifications et enseignements à partir du cas belge
Halleux, Jean-Marie ULg

Scientific conference (2013, September 30)

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See detailLes défis de L’Etat social. De quoi l’éthique est-elle le nom ?
Frère, Bruno ULg; Delruelle, Edouard ULg

Article for general public (2015)

Detailed reference viewed: 38 (0 ULg)
See detailLes défis de la mondialisation pour l’enseignement des langues
Defays, Jean-Marc ULg

Scientific conference (2014, April 09)

Detailed reference viewed: 25 (3 ULg)
See detailles défis de la recherche en lutte biologique contre les maladies des fruits récoltés
Jijakli, Haissam ULg

Scientific conference (2009, January)

Detailed reference viewed: 18 (1 ULg)
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See detailLes défis de la revalidation d'une dysfonction érectile depuis l'arrivée des traitements actifs par voie orale
Andrianne, Robert ULg; Debois, Christine ULg; Mormont, Christian ULg et al

in Références en Gynécologie Obstétrique (2004), 10

Now, it is relatively easy to restore normal erections in many patients thanks to active oral drugs. However, full success is a complicated process, which is not limited to mechanical response to ... [more ▼]

Now, it is relatively easy to restore normal erections in many patients thanks to active oral drugs. However, full success is a complicated process, which is not limited to mechanical response to treatment. Treatment discontinuation rates are much higher than expected, given efficacy of these drugs, because other factors such as partner relationship and couple well being are implicated. [less ▲]

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See detailLes défis des familles recomposées
Guillaume, Jean-François ULg

in Observatoire : Revue d'Action Sociale & Médico-Sociale (1994), 6(novembre-décembre), 30-36

Dans un contexte de croissance des recompositions conjugales après divorce, la prise en charge des tâches parentales constitue un défi inédit, où il s'agit pour les uns de retrouver des repères bien ... [more ▼]

Dans un contexte de croissance des recompositions conjugales après divorce, la prise en charge des tâches parentales constitue un défi inédit, où il s'agit pour les uns de retrouver des repères bien connus, et pour les autres, d'imaginer un nouvel ordre éducatif. Une nouvelle histoire doit alors être créée, et dans ce "bricolage", chacun ne dispose pas des mêmes ressources pour trouver sa place. [less ▲]

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See detailLes défis du droit romain et du droit privé comparé
Gerkens, Jean-François ULg

in Gosselin, Antoinette; Langevin, Caroline; Wautelet, Patrick (Eds.) L'avenir de la recherche: Réflexions à l'occasion des leçons inaugurales 2010 (2010)

Detailed reference viewed: 98 (10 ULg)