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See detailDétection et caractérisation de peptides obliques au sein de protéines amyloïdogéniques
Crowet, Jean-Marc ULg

Doctoral thesis (2008)

The study of amyloidogenic proteins is of interest in biochemistry because these proteins undergo conformational changes and aggregation. Both processes are largely implicated in several diseases ... [more ▼]

The study of amyloidogenic proteins is of interest in biochemistry because these proteins undergo conformational changes and aggregation. Both processes are largely implicated in several diseases including Alzheimer’s, Parkinson’s or Creutzfeldt-Jakob’s disease. These phenomena are not completely understood, either at a structural or energetical point of view. Tilted peptides are short protein fragment (11 to 19 residues) that adopt a tilted orientation when inserted into biological membranes and destabilise them. Recently, tilted peptides have been detected in two amyloidogenic proteins involved in neurodegenerative diseases; the amyloid β peptide responsible for Alzheimer’s disease, and the PrP protein that causes Creutzfeldt-Jakob’s disease. Tilted peptides could be responsible for the neurotoxic effects of these proteins. Due to their destabilising properties, they could interact directly with the membrane leading to cell death. Tilted peptides could also be involved in the transconformational process of the proteins. The aim of this work is to detect tilted fragments in other amyloidogenic proteins by molecular modelling and to study some of these peptides experimentally to evidence their lipid destabilizing properties, their structure and their toxicity. In addition, this work enable the design of an automatic method of detection for tilted peptides. Twenty-two tilted peptides from 18 different proteins have been detected among 53 amyloidogenic proteins and 7 peptides were tested experimentally. The results support the hypothesis that some tilted peptides could be involved in transconformational processes and/or cytotoxicity related to amyloidogenic proteins. [less ▲]

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See detailDétection et estimation du niveau de la maladie rénale chronique
Delanaye, Pierre ULg; Cavalier, Etienne ULg; Mariat, Christophe et al

in Revue Médicale de Liège (2009), 64(2), 73-78

L’insuffisance rénale voit son incidence augmenter. Un diagnostic précoce et précis est requis. Le diagnostic de l’insuffisance rénale repose sur la mesure du débit de filtration glomérulaire. Les ... [more ▼]

L’insuffisance rénale voit son incidence augmenter. Un diagnostic précoce et précis est requis. Le diagnostic de l’insuffisance rénale repose sur la mesure du débit de filtration glomérulaire. Les formules basées sur la créatinine pour déterminer ce débit ont, par rapport à la créatinine sérique seule, apporté un plus mais, dans certaines situations, elles fournissent des résultats erronés. Le recours à la mesure de la clairance de créatinine sur une récolte d’urines de 24 heures peut être, dans ces situations où les caractéristiques anthropométriques sont hors normes, utile, mais avec toujours la difficulté d’une récolte minutée correcte. Si une mesure précise du débit de filtration glomérulaire est absolument nécessaire, une méthode de référence doit être choisie telle celle au chrome EDTA ou à l’iohexol. Enfin, tout bilan néphrologique doit, outre le dosage de créatinine, comprendre au moins une analyse d’urine à la tigette. Toute positivité pour la protéinurie lors de ce test mérite de réaliser un dosage quantitatif de la protéinurie soit sur 24 heures, soit plus aisément sur un échantillon [less ▲]

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See detailDétection et localisation d’endommagements structuraux par la méthode des sous-espaces, le modèle de Kalman et la méthode d’analyse en composantes principales
Yan, Ai-Min; De Boe, Pascal; Golinval, Jean-Claude ULg

in Mécanique & Industries (2006), 7

This paper deals with the application of statistical process control techniques for damage diagnosis based on vibration measurements. The first approach considered in this work is based the Stochastic ... [more ▼]

This paper deals with the application of statistical process control techniques for damage diagnosis based on vibration measurements. The first approach considered in this work is based the Stochastic Subspace Identification (SSI) algorithm, from which a Kalman model is constructed to fit the measured response histories of the undamaged (reference) structure. This model may be used to make a prediction of the newly measured responses. The residual error between the model predictions and the actual measurements is defined as a damage-sensitive feature. Outlier statistics provides then a quantitative indicator of damage. The advantage of the method is that model extraction has to be performed only once using the reference data and that no further modal identification is needed. Thus on-line structural health monitoring may easily be realized. In the second approach, principal component analysis (PCA) of the sensor time-responses is used to extract principal directions (i.e. features), which define a subspace that is representative of the dynamics of the instrumented structure. Any change in the response of a single sensor affects the subspace spanned by the complete sensor response set. It follows that the subspace corresponding to the current state of the structure can be compared to the subspace of the initial state of the structure, assumed to be healthy, in order to diagnose possible damage. Principal component analysis may also be performed for every potential subset of damaged sensors in order to identify the involved sensor, and, therefore, the damaged substructure. [less ▲]

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See detailDetection of a new mutation responsible for primary ciliary dyskinesia in a pedigree of old English Sheepdogs
Merveille, Anne-Christine ULg; Bataille, G.; Davis, E. et al

in 19h ECVIM Meeting - Porto, Portugal - 8-10 septembre 2009 (2009, September)

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See detailDetection of a single synchronous growth change in several tree-ring series of south-east France
Nicault, A.; Boreux, Jean-Jacques ULg; Rathegeber, C.

in Proceedings of the 6th international conference on dendrochronology (2002)

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See detailDetection of a Stellar Occultation by (87) Sylvia I (Romulus)
Berthier, J.; Frappa, E.; Klotz, A. et al

in Central Bureau Electronic Telegrams (2013)

Report of the successful stellar occultations by the triple minor-planet system (87) Sylvia on Jan. 6.06742 UT of the star HD 244394 (mag 10.8). twelve positive chords of the occultation by the primary ... [more ▼]

Report of the successful stellar occultations by the triple minor-planet system (87) Sylvia on Jan. 6.06742 UT of the star HD 244394 (mag 10.8). twelve positive chords of the occultation by the primary, lasting between 4 s and 26 s, were successfully reported. Observations recorded by 4 teams permitted the detection of a brief (0.5-2 s) stellar occultation at the expected position of the large satellite Romulus. Preliminary analysis of the Romulus chords suggest that the shape of the satellite is extremely elongated or irregular (a/b > 2), with a major axis oriented toward the primary, and an equivalent diameter between 18 and 28 km. [less ▲]

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See detailDetection of a transit of the super-Earth 55 Cnc e with Warm Spitzer
Demory, B.-O.; Gillon, Michaël ULg; Deming, D. et al

in Astronomy and Astrophysics (2011), 533

We report on the detection of a transit of the super-Earth 55 Cnc e with warm Spitzer in IRAC's 4.5 μm band. Our MCMC analysis includes an extensive modeling of the systematic effects affecting warm ... [more ▼]

We report on the detection of a transit of the super-Earth 55 Cnc e with warm Spitzer in IRAC's 4.5 μm band. Our MCMC analysis includes an extensive modeling of the systematic effects affecting warm Spitzer photometry, and yields a transit depth of 410 ± 63 ppm, which translates to a planetary radius of 2.08+0.16-0.17 R_oplus as measured in IRAC 4.5 μm channel. A planetary mass of 7.81-0.53+0.58 M_oplus is derived from an extensive set of radial-velocity data, yielding a mean planetary density of 4.78-1.20+1.31 g cm-3. Thanks to the brightness of its host star (V = 6, K = 4), 55 Cnc e is a unique target for the thorough characterization of a super-Earth orbiting around a solar-type star. [less ▲]

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See detailDetection of abrupt events within a jet engine health monitoring procedure
Borguet, Sébastien ULg; Léonard, Olivier ULg

in Proceedings of the 19th ISABE Conference (2009, September)

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See detailDetection of accase target-site resistant Alopecurus myosuroides huds (black-grass) in Belgian populations
Maréchal, Pierre-Yves ULg; Henriet, François; Bodson, Bernard ULg

in Communications in Agricultural and Applied Biological Sciences (2009, May 19)

Black-grass is a common grass weed, widely spread in Northern Europe and also in Belgium. For ages, it has been an increasing problem in industrial crops, especially winter cereals. The first case of ... [more ▼]

Black-grass is a common grass weed, widely spread in Northern Europe and also in Belgium. For ages, it has been an increasing problem in industrial crops, especially winter cereals. The first case of resistance in Belgium was reported in 1996 by Robert Bulcke (Eelen et al., 1996). Yet the resistance mechanism was not specified. Since then, no more information was published about the evolution Belgium, while research continued in the United Kingdom and in France. Moreover, during the last decade, progress in molecular biology allowed to highlight the mechanism of target-site resistance. A simple PCR method allows to detect the mutation conferring resistance to herbicide. After two years of resistance monitoring in Belgium, mostly in the Walloon part, some populations have been clearly identified as highly resistant to ACCase inhibitor. These populations have been tested by molecular biology so as to detect the single nucleotide polymorphism (SNP) involved in this case. The method employed was the Polymerase Chain Reaction Allele Specific Assays (PASA: Délye, 2002a) for the mutation Ile-1781-Leu that confers a target-site resistance to ACCase inhibitors. Those analyses were performed on plant material issued from bioassays, either in glasshouses or in Petri dishes. Leaves have been collected from plants which survived a fenoxaprop-P treatment applied in a glasshouse single dose assay. Seedlings from resistant populations grown in Petri dishes containing either fenoxaprop-P or cycloxydim provided the second type of sample. Ile1781 mutants were discovered within three populations. Each mutant plant was heterozygote. Five of those samples have been sequenced to confirm PASA results and everyone was matching. Moreover, they were all issued from Petri dishes containing cycloxydim, known to be unaffected by enhanced metabolism, confirming that theses populations are indeed target-site resistant. [less ▲]

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See detailDetection of Adverse Events in Administrative Data
Gillet, Pierre ULg; Kolh, Philippe ULg; Sermeus, W. et al

Report (2008)

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See detailDetection of aggregative behaviour in binary choice experiments
Brostaux, Yves ULg

Poster (2011, August 22)

Aggregative behaviour can be defined as a natural tendency for individuals to cluster in space into groups of varying sizes. In entomology, this behaviour can be detected in binary choice experiments ... [more ▼]

Aggregative behaviour can be defined as a natural tendency for individuals to cluster in space into groups of varying sizes. In entomology, this behaviour can be detected in binary choice experiments. Analysis of these experiments reveals specific characteristics (unequal counts between repetitions, dependence of individual choices) that make the traditional adjustment tests fail. We demonstrate that the use of generalized linear models can circumvent these pitfalls and deliver a reliable diagnosis on the social behaviour of the studied invertebrates. The strength of this behaviour can then be evaluated through the use of common correlation models. [less ▲]

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See detailDetection of Alternaria and Cladosporium DNA in nasal mucosa from dogs with idiopathic lymphoplasmacytic rhinitis
Mercier, Elise ULg; Peters, Iain; Billen, Frédéric ULg et al

in Proceedings of the 21st ECVIM-CA congress (2011, September 06)

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See detailDetection of Anaplasma phagocytophilum in Dermacentor reticulatus ticks
Wirtgen, Marc ULg; Nahayo, A.; Linden, Annick ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2011), 168(9), 248

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See detailDetection of Angiostrongylus vasorum in the bronchoalveolar lavage fluid or faeces of coughing and healthy dogs in Belgium.
Canonne-Guibert, Morgane ULg; Roels, Elodie ULg; Caron, Yannick ULg et al

in Proceedings of the 24th Ecvim Meeting, Mainz, Germany - 4-6 September 2014 (2014, September)

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See detailDetection of anti-laminin antibodies in sera by latex agglutination.
Bernard, A. M.; Foidart, Jean-Michel ULg; Mahieu, P. et al

in Clinical Chemistry (1986), 32(8), 1468-72

We describe a latex particle agglutination assay for detecting circulating antibodies against laminin, a noncollagenous glycoprotein of basement membranes. Polystyrene latex particles on which laminin has ... [more ▼]

We describe a latex particle agglutination assay for detecting circulating antibodies against laminin, a noncollagenous glycoprotein of basement membranes. Polystyrene latex particles on which laminin has been adsorbed are incubated with serum for about 25 min at 42-45 degrees C. The agglutination is then measured by counting residual unagglutinated particles. Polyethylene glycol 6000 enhances the agglutination. The assay is fully automated, yielding results in about 45 min, for 50 samples per hour. Addition of purified laminin abolishes the agglutination of laminin-coated particles in practically all positive sera. The anti-laminin antibody titers obtained by this latex immunoassay and by radioimmunoassay correlated well in 161 sera from patients with suspected or established renal diseases. The agglutination assay more frequently gave positive results for cases of glomerulonephritis with linear deposits (20/22 cases) than for glomerulonephritis with granular deposits (7/68) or glomerulonephritis with no glomerular deposits (2/13). The finding of low anti-laminin antibody titers in sera from about 15% (34/230) of the healthy subjects suggests that these autoantibodies are pathogenic only in certain circumstances. [less ▲]

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See detailDetection of antibodies against Schmallenberg virus in wild boars, Belgium, 2010-2012
Desmecht, Daniel ULg; Garigliany, Mutien-Marie ULg; Beer, Martin et al

in Lecoq, Yves (Ed.) 31th Congress of the International Union of Game Biologists (2013, August 27)

In the summer/fall of 2011, a nonspecific febrile syndrome characterized by hyperthermia and drop in milk production with occasional reports of watery diarrhea and abortion was reported among dairy cows ... [more ▼]

In the summer/fall of 2011, a nonspecific febrile syndrome characterized by hyperthermia and drop in milk production with occasional reports of watery diarrhea and abortion was reported among dairy cows on farms in northwestern Europe. Further, in November 2011, an enzootic outbreak of malformed neonates emerged in several European countries, with stillbirth and birth at term of lambs, kids and calves with neurological signs or malformations of the head, spine, or limbs. Both syndromes were associated with the presence in the blood (adults) or in the central nervous system (newborns) of a new Shamonda/Sathuperi-like orthobunyavirus, provisionally named Schmallenberg virus (SBV) after the town in Germany where the first positive clinical samples were identified. Defining as precisely as possible the host range of the newcomer is a key point to predict the outcome of the emergence of SBV disease in Europe. In this respect, it must be pointed out that orthobunyaviruses infect more animal species than those in which the foetus is damaged. Recently, serological evidence for SBV infection in wild ruminant species (Cervus elaphus and Capreolus capreolus) was reported (Linden et al., 2012). In the present study, the objective was to seek after serological evidence of SBV infection among wild boars living in a geographical area where exposure to infected insect vectors was high in 2011, as judged from the very high seroprevalence reported among cattle in that region. About 700 animals were sampled during the 2010-2012 hunting seasons. All serum samples collected during the fall of 2010 were seronegative. On the contrary, apparent seroprevalence among wild boars in 2011 was ~27% and started to decline in 2012 (~11%). Acquired immunity against the new virus was thus already very high in the wild boar populations sampled in the fall 2011, suggesting that the new virus had quickly spread throughout the region since its emergence about 250 km northeast in the late summer 2011. The drop in seroprevalence recorded in 2012 suggests that the virus was no more circulating in the region. [less ▲]

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See detailDetection of antigenic heterogeneity in feline coronavirus nucleocapsid in feline pyogranulomatous meningoencephalitis.
Poncelet, Luc; Coppens, Angélique; Peeters, Dominique ULg et al

in Veterinary Pathology (2008), 45(2)

Detailed reference viewed: 9 (2 ULg)