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See detailDeletion of a cyclic AMP receptor protein homologue diminishes germination and affects morphological development of Streptomyces coelicolor
Derouaux, Adeline ULg; Halici, S.; Nothaft, H. et al

in Journal of Bacteriology (2004), 186(6), 1893-1897

Open reading frame SCO3571 of Streptomyces coelicolor encodes a protein of the cyclic AMP (cAMP) receptor protein (CRP) superfamily of regulatory proteins. A mutant revealed a dramatic defect in ... [more ▼]

Open reading frame SCO3571 of Streptomyces coelicolor encodes a protein of the cyclic AMP (cAMP) receptor protein (CRP) superfamily of regulatory proteins. A mutant revealed a dramatic defect in germination, followed by growth delay and earlier sporulation. This phenotype correlates with those of an adenylate cyclase (cya) mutant that cannot synthesize cAMP. This finding suggests that S. coelicolor may use a Cya-cAMP-CRP system to trigger complex physiological processes such as morphogenesis. [less ▲]

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See detailDeletion of cysteine cathepsins B or L yields differential impacts on murine skin proteome and degradome
Tholen, Stefan; Biniossek, Martin L; Gansz, Martina et al

in Molecular & Cellular Proteomics (2013), 12(3), 611-25

Numerous studies highlight that concerted proteolysis is essential for skin morphology and function. The cysteine protease cathepsin L (Ctsl) has been implicated in epidermal proliferation and ... [more ▼]

Numerous studies highlight that concerted proteolysis is essential for skin morphology and function. The cysteine protease cathepsin L (Ctsl) has been implicated in epidermal proliferation and desquamation as well as in hair cycle regulation. In stark contrast, mice deficient for cathepsin B (Ctsb) do not display an overt skin phenotype. To understand the systematic consequences of deleting Ctsb or Ctsl, we determined protein abundances of > 1300 proteins and proteolytic cleavage events in skin samples of wild-type, Ctsb-/- and Ctsl-/- mice by mass spectrometry based proteomics. Both protease deficiencies revealed distinct quantitative changes in proteome composition. Ctsl-/- skin revealed increased levels of the cysteine protease inhibitors cystatin B and cystatin M/E, increased cathepsin D and accumulation of the extracellular glycoprotein periostin. Immunohistochemistry located periostin predominantly in hypodermal connective tissue of Ctsl-/- skin. Proteomic identification of proteolytic cleavage sites within skin proteins revealed numerous processing sites that are underrepresented in Ctsl-/- or Ctsb-/- samples. Notably, few of the affected cleavage sites shared the canonical Ctsl or Ctsb specificity, providing further evidence for a complex proteolytic network in the skin. Novel processing sites in proteins such as dermokine and Notch-1 were detected. Simultaneous analysis of acetylated protein N-termini showed prototypical mammalian N-alpha acetylation. These results illustrate an influence of both Ctsb and Ctsl on the murine skin proteome and degradome with the phenotypic consequences of the absence of either protease differing considerably. [less ▲]

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See detailDeletion of exons 1-3 of the MEN1 gene in a large Italian family causes the loss of menin expression.
Zatelli, Maria Chiara; Tagliati, Federico; Di Ruvo, Mauro et al

in Familial cancer (2014)

Multiple endocrine neoplasia type 1 (MEN1) syndrome is an autosomal dominant disease, characterized by parathyroid adenomas, endocrine gastroenteropancreatic tumors and pituitary adenomas, due to ... [more ▼]

Multiple endocrine neoplasia type 1 (MEN1) syndrome is an autosomal dominant disease, characterized by parathyroid adenomas, endocrine gastroenteropancreatic tumors and pituitary adenomas, due to inactivating mutations of the MEN1 gene (chromosome 11q13). MEN1 mutations are mainly represented by nonsense, deletions/insertions, splice site or missense mutations that can be detected by direct sequencing of genomic DNA. However, MEN1 patients with large heterozygous deletions may escape classical genetic screening and may be misidentified as phenocopies, thereby hindering proper clinical surveillance. We employed a real-time polymerase chain reaction application, the TaqMan copy number variation assay, to evaluate a family in which we failed to identify an MEN1 mutation by direct sequencing, despite a clear clinical diagnosis of MEN1 syndrome. Using the TaqMan copy number variation assay we identified a large deletion of the MEN1 gene involving exons 1 and 2, in three affected family members, but not in the other nine family members that were to date clinically unaffected. The same genetic alteration was not found in a group of ten unaffected subjects, without family history of endocrine tumors. The MEN1 deletion was further confirmed by multiplex ligation-dependent probe amplification, which showed the deletion extended from exon 1 to exon 3. This new approach allowed us to correctly genetically diagnose three clinical MEN1 patients that were previously considered as MEN1 phenocopies. More importantly, we excluded the presence of genetic alterations in the unaffected family members. These results underline the importance of using a variety of available biotechnology approaches when pursuing a genetic diagnosis in a clinically suggestive setting of inherited endocrine cancer. [less ▲]

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See detailDeletion of Melanin-Concentrating Hormone Receptor-1 gene accentuates D-amphetamine-induced psychomotor activation but neither the subsequent development of sensitization nor the expression of conditioned activity in mice
Tyhon, Amélie ULg; Lakaye, Bernard ULg; Grisar, Thierry ULg et al

in Pharmacology, Biochemistry & Behavior (2008), 88

The present study aimed to test the hypothesis that mice lacking the MCHR1 receptor (Melanin-Concentrating Hormone Receptor-1) present an elevated vulnerability towards the neurobehavioural effects of D ... [more ▼]

The present study aimed to test the hypothesis that mice lacking the MCHR1 receptor (Melanin-Concentrating Hormone Receptor-1) present an elevated vulnerability towards the neurobehavioural effects of D-amphetamine, presumably due to previously established up-regulations of dopamine D1 receptors in these mice. We examined the psychomotor effects of five once-daily injections of 1.5 and 3 mg/kg D-amphetamine (i.p.) or ten once-daily injections of 2.25 mg/kg D-amphetamine in knockout (KO) mice lacking the MCHR1 receptor. The first injection of Damphetamine induced a greater psychomotor response amongst the KO mice at 2.25 and 3.0 mg/kg. On all subsequent D-amphetamine injections, KO mice still showed greater levels of psychomotor activity than the WT mice, but with no between-genotype difference in the rate of development of sensitization (similar slopes of the curves). Furthermore, 24 h after the last injection of 2.25 mg/kg D-amphetamine both genotypes exhibited a significant post-sensitization conditioned activity. Thus, MCHR1 receptors are likely not deeply involved in the mechanisms of induction of sensitization and related conditioned activity induced by D-amphetamine, albeit our results confirm a contribution of these receptors to the mechanisms of the acute effects of that drug, possibly via an inhibitory action on the dopaminergic mesolimbic system. Our results do not support the hypothesis of a functional contribution of MCHR1 receptors to the addictive effects of D-amphetamine [less ▲]

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See detailDeletion of six ORFs from chromosome X
Vandenbol, Micheline ULg; Hilger, François; Portetelle, Daniel ULg

Poster (1997)

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See detailDeletion of six ORFs from chromosome X
Vandenbol, Micheline ULg; Hilger, F.; Portetelle, Daniel ULg

in Second Eurofan Meeting (1997)

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See detailDeletion of the 5'-ABL region: a recurrent anomaly detected by fluorescence in situ hybridization in about 10% of Philadelphia-positive chronic myeloid leukaemia patients.
Herens, Christian ULg; Tassin, Françoise ULg; Lemaire, Véronique ULg et al

in British Journal of Haematology (2000), 110(1), 214-6

Inclusion of the BCR-ABL ES probe in routine cytogenetics led to the identification of a subgroup of Philadelphia positive (Ph+) chronic myeloid leukaemia patients characterized by a 5'-ABL deletion. This ... [more ▼]

Inclusion of the BCR-ABL ES probe in routine cytogenetics led to the identification of a subgroup of Philadelphia positive (Ph+) chronic myeloid leukaemia patients characterized by a 5'-ABL deletion. This anomaly was observed in 5/51 cases (9.8%). Cytological and clinical data suggest that the 5'-ABL deletion may be associated with dysplastic features of polymorphonuclear cells and metamyelocytes and a short chronic phase duration. [less ▲]

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See detailDeletion of Xpter encompassing the SHOX gene and PAR1 region in familial patients with Leri-Weill Dyschondrosteosis syndrome.
Mutesa, L.; Vanbellinghen, Jean-François ULg; Hellin, Anne-Cécile ULg et al

in Genetic Counseling (Geneva, Switzerland) (2009), 20(1), 9-17

Heterozygote deletions or mutations of pseudoautosomal 1 region (PAR1) encompassing the short stature homeobox-containing (SHOX) gene cause Leri-Weill Dyschondrosteosis (LWD), which is a dominantly ... [more ▼]

Heterozygote deletions or mutations of pseudoautosomal 1 region (PAR1) encompassing the short stature homeobox-containing (SHOX) gene cause Leri-Weill Dyschondrosteosis (LWD), which is a dominantly inherited osteochondroplasia characterized by short stature with mesomelic shortening of the upper and lower limbs and Madelung deformity of the wrists. SHOX is expressed by both sex chromosomes in males and females and plays an important role in bone growth and development. Clinically, the LWD expression is variable and more severe in females than males due to sex differences in oestrogen levels. Here, we report two familial cases of LWD with a large Xp terminal deletion (approximately 943 kb) of distal PAR1 encompassing the SHOX gene. In addition, the proband had mental retardation which appeared to be from recessive inheritance in the family. [less ▲]

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See detailDeletions of the 3 ' BCR and 5 ' ABL regions in patients with Philadelphia-positive chronic myeloid leukemia: a one-step process occurring in about 10% of the cases without any evidence of genetic instability in the target cells
Fournier, M.; Lacrosse, S.; Jamar, Mauricette ULg et al

in Cancer Genetics & Cytogenetics (2005), 160(2), 184-187

Deletions of the 5' ABL region adjacent to the t(9;22)(q34;q11) have recently been reported in 8-32.7% of patients with chronic myeloid leukemia (CML). The deletions were visualized with fluorescence in ... [more ▼]

Deletions of the 5' ABL region adjacent to the t(9;22)(q34;q11) have recently been reported in 8-32.7% of patients with chronic myeloid leukemia (CML). The deletions were visualized with fluorescence in situ hybridization using, in the majority of the cases, the Vysis LSI BCR/ABL ES (extra signal) probe. In our series, 10 of 99 CML patients (10.1%) were characterized by a 5'ABL deletion. We show that 3' BCR losses are observed in nearly all the cases with 5' ABL deletions. Moreover, the different genetic events (Philadelphia chromosome formation; 5' ABL and 3' BCR deletions) occur simultaneously in a one-step process without any evidence for genetic instability in the target bone marrow cells. (c) 2005 Elsevier Inc. All rights reserved. [less ▲]

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See detailDeleuze : une esthétique baroque
Steinmetz, Rudy ULg

in L'atelier d'esthétique (Ed.) Esthétique et philosophie de l'art (2002)

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See detailDeliberative Polls and Citizens’ Assemblies
Reuchamps, Min ULg

Scientific conference (2008, October 13)

In the first part of this presentation, the research techniques of delibarative polls and citizens' assemblies are presented and discussed. The second part presents the preliminary results from a small ... [more ▼]

In the first part of this presentation, the research techniques of delibarative polls and citizens' assemblies are presented and discussed. The second part presents the preliminary results from a small version of the citizens' assemblies technique applied in the context of deep-divided societies such as Belgium and Canada. [less ▲]

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See detailDélimitation des hinterlands des agglomérations urbaines au départ d’une image de télédétection
Donnay, Jean-Paul ULg

in Revue Belge de Géographie (1995), 3-4(59), 325-331

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See detailDélimitation, intensité et spécialisation des entités commerciales. Considérations méthodologiques
Merenne-Schoumaker, Bernadette ULg

in Bulletin de la Société Géographique de Liège (1982), (18), 77-89

The aim of this article is to confront various methods that are used to delimit commercial entities, to mesure the intensity of commercial activity of the various business spaces and sub-spaces and to ... [more ▼]

The aim of this article is to confront various methods that are used to delimit commercial entities, to mesure the intensity of commercial activity of the various business spaces and sub-spaces and to translate their specialisation. By showing the limits and performances of the various index numbers and processes, it tries to propose adequate methodological tools for a more precise analysis of the commercial function. [less ▲]

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See detailDélimiter des forêts communautaires : une approche par contraintes multiples
Schippers, C.; Bracke, C.; Ndouna Ango, A. et al

in Vermeulen, Cédric; Doucet, Jean-Louis (Eds.) Les premières forêts communautaires du Gabon : Récits d'une expérience pilote (2008)

La délimitation des forêts communautaires est une des premières étapes dans la mise en place de celles-ci. Ce processus étapes dans la mise en place de celles-ci. Ce processus que coutumières. Cet article ... [more ▼]

La délimitation des forêts communautaires est une des premières étapes dans la mise en place de celles-ci. Ce processus étapes dans la mise en place de celles-ci. Ce processus que coutumières. Cet article présente une méthodologie de délimitation participative, techniquement accessible aux communautés villageoises et respectant ces différentes contraintes. L’approche suivie intègre d’une part les obligations légales (limites des permis forestiers, dispositions réglementaires…) et d’autre part l’occupation actuelle de l’espace par les populations villageoises ainsi que les attentes de celles-ci, tout en veillant à limiter les conflits éventuels. Deux cas de délimitation de forêts communautaires pilotes au Gabon sont abordés, le cas du village de La Scierie et le cas du regroupement de villages de Ebe Messe Melane. [less ▲]

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