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See detailA comparison of the training value of two types of anesthesia simulators: Computer screen-based and mannequin-based simulators
Nyssen, Anne-Sophie ULg; Larbuisson, Robert ULg; Janssens, Marc ULg et al

in Anesthesia and Analgesia (2002), 94(6), 1560-1565

In this study, we compared two different training simulators (the computer screen-based simulator versus the full-scale simulator) with respect to training effectiveness in anesthesia residents ... [more ▼]

In this study, we compared two different training simulators (the computer screen-based simulator versus the full-scale simulator) with respect to training effectiveness in anesthesia residents. Participants were evaluated in the management of a simulated preprogrammed scenario of anaphylactic shock using two variables: treatment score and diagnosis time. Our results showed that simulators can contribute significantly to the improvement of performance but that learning in treating simulated crisis situations such as anaphylactic shock did not significantly vary between full-scale and computer screen-based simulators. Consequently, the initial decision on whether to use a full-scale or computer screen-based training simulator should be made on the basis of cost and learning objectives rather than on the basis of technical or fidelity criteria. Our results support the contention that screen-based simulators are good devices to acquire technical skills of crisis management. Mannequin-based simulators would probably provide better training for behavioral aspects of crisis management, such as communication, leadership, and interpersonal conflicts, but this was not tested in the current study. IMPLICATIONS: We compared two different training simulators (computer screen-based versus full-scale) for training anesthesia residents to better document the effectiveness of such devices as training tools. This is an important issue, given the extensive use and the high cost of mannequin-based simulators in anesthesiology. [less ▲]

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See detailComparison of the transient responses of Escherichia coli to a glucose pulse of various intensities
Sunya, Sirichai; Delvigne, Frank ULg; Uribellarea, Jean-Louis et al

in Applied Microbiology & Biotechnology (2012), 95(4), 1021-1034

tDynamic stimulus-responses of Escherichia coli DPD2085, yciG::LuxCDABE reporter strain, to glucose pulses of different intensities (0.08, 0.4 and 1 gL −1) were compared using glucose-limited chemostat ... [more ▼]

tDynamic stimulus-responses of Escherichia coli DPD2085, yciG::LuxCDABE reporter strain, to glucose pulses of different intensities (0.08, 0.4 and 1 gL −1) were compared using glucose-limited chemostat cultures at dilution rate close to 0.15 h −1. After at least five residence times, the steady-state cultures were disturbed by a pulse of glucose, engendering conditions of glucose excess with concomitant oxygen limitation. In all conditions, glucose consumption, acetate and formate accumulations followed a linear relationship with time. The resulting specific uptake and production rates as well as respiratory rates were rapidly increased within the first seconds, which revealed a high ability of E. coli strain to modulate its metabolism to a new environment. For transition from glucose-excess to glucoselimited conditions, the cells rapidly re-established its pseudo-steady state. The dynamics of transient responses at the macroscopic viewpoint were shown to be independent on the glucose pulse intensity in the tested range. On the contrary, the E. coli biosensor yciG::luxCDABE revealed a transcriptional induction of yciG gene promoter depending on the quantities of the glucose added, through in situ and online monitoring of the bioluminescence emitted by the cells. Despite many studies describing the dynamics of the transient response of E. coli to glucose perturbations, it is the first time that a direct comparison is reported, using the same experimental design (strain, medium and experimental set up), to study the impact of the glucose pulse intensity on the dynamics of microbial behaviour regarding growth, respiration and metabolite productions. [less ▲]

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See detailComparison of the value of measurement of serum galactomannan and Aspergillus-specific antibodies in the diagnosis of canine sino-nasal aspergillosis.
Billen, Frédéric ULg; Peeters, Dominique ULg; Peters, I. R. et al

in Veterinary Microbiology (2009), 133(4), 358-65

Serology is currently used for the diagnosis of canine sino-nasal aspergillosis (SNA). However, the accuracy of serological testing using commercially available, standardized purified antigen preparations ... [more ▼]

Serology is currently used for the diagnosis of canine sino-nasal aspergillosis (SNA). However, the accuracy of serological testing using commercially available, standardized purified antigen preparations of Aspergillus (CAPurAspAg) has only been poorly documented. The aim of the present study was to assess the diagnostic value of an agar-gel double immunodiffusion (AGDD) test and an anti-Aspergillus IgG ELISA, using CAPurAspAg and the commercially available Platelia test for the detection of serum galactomannan. Sera from 17 dogs with SNA, 18 dogs with a nasal tumour (NT), 11 dogs with lymphoplasmacytic rhinitis (LPR) and 33 control dogs were tested with the 3 methods. AGDD result was positive in 76.5% of dogs with SNA, whereas all sera from dogs with non-fungal nasal disease and control dogs were negative. A positive IgG ELISA result was obtained in 88% of dogs with SNA and in 18% of dogs with LPR. All patients with NT and control dogs had a negative IgG ELISA result. The Platelia test was positive in 24% of dogs with SNA, 11% of dogs with NT, 9% of dogs with LPR and 24% of control dogs. The results of this study suggest that (1) the detection of serum Aspergillus-specific antibodies with AGDD or ELISA, using CAPurAspAg, provides excellent specificity and good sensitivity, (2) the specificity is higher for AGDD (100%) than for ELISA (96.8%) while sensitivity is higher for ELISA (88.2%) than for AGDD (76.5%) and (3) serum galactomannan quantification with the Plateliat test is unreliable for the diagnosis of canine SNA. [less ▲]

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See detailComparison of the wear behavior of high speed steel grades obtained from conventional casting and laser cladding
Hashemi, Seyedeh Neda ULg; Dedry, Olivier ULg; Lecomte-Beckers, Jacqueline ULg et al

in Materiaux et tribologie jift 2014 (2015)

The wear characteristics of three high speed steel grades were studied, one of them being obtained from a conventional casting process and two others originated from a laser cladding route. Tribological ... [more ▼]

The wear characteristics of three high speed steel grades were studied, one of them being obtained from a conventional casting process and two others originated from a laser cladding route. Tribological tests were carried out at room temperature using a “pin-on-disc” device. The setting of the wear test parameters together with the microstructure of the studied materials seems to strongly influence the subsequent abrasion and wear mechanisms. The results show that the laser cladded high speed steel materials resist better to wear thanks to their ultrafine microstructure compared to the conventional cast material. [less ▲]

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See detailComparison of the wear behavior of high speed steel grades obtained from conventional casting and laser cladding
Hashemi, Seyedeh Neda ULg; Lecomte-Beckers, Jacqueline ULg; Montrieux, Henri-Michel ULg et al

Conference (2015, September)

Tools Steels are alloys which withstand severe mechanical and physicochemical stresses in service. Therefore their alloying design that involved both the original chemical composition and the casting ... [more ▼]

Tools Steels are alloys which withstand severe mechanical and physicochemical stresses in service. Therefore their alloying design that involved both the original chemical composition and the casting route is crucial in order to achieve a tailored microstructure exhibiting enhanced wear performances. Tools steels obtained from conventional casting processes had received lot of attention so far as they yield typical microstructure composed of a quasi-continuous network of coarse grain boundary carbides with grain size ranging between 20 to 200 µm. Direct energy deposition applied to Tools Steels represents a new emerging technique that may allow ultrafine grained microstructures due to the higher cooling rates achieved especially in the solidification range. In this paper, four tool steels grades were studied, one of them being obtained from a conventional casting process and the other ones originated from the direct energy deposition. Differential Thermal Analysis helps enhancing the solidification sequence of the studied alloys, while their microstructure after subsequent heat treatment was characterized by the means of both optical and electron microscopes together with hardness measurements. Tribological tests carried out at room temperature and at high temperature were performed while using a “pin-on-disc” device. Based on the friction coefficient and the wear rate, the wear performances of the tool steel were determined and compared with one another. The influence of metallurgical features (the grain size, or the nature, the size and the distribution of carbides) on the wear behavior was also enhanced. The setting of the wear test parameters together with the microstructure of the studied materials seems to strongly influence the subsequent abrasion and wear mechanisms. [less ▲]

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See detailComparison of Thiopentone/Guaifenesin, Ketamine/Guaifenesin and Ketamine/Midazolam for the Induction of Horses to Be Anaesthetised with Isoflurane
Gangl, M.; Grulke, Sigrid ULg; Detilleux, Johann ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2001), 149(5), 147-51

Forty-eight horses subjected to elective surgery were randomly assigned to three groups of 16 horses. After premedication with 0.1 mg/kg acepromazine intramuscularly and 0.6 mg/kg xylazine intravenously ... [more ▼]

Forty-eight horses subjected to elective surgery were randomly assigned to three groups of 16 horses. After premedication with 0.1 mg/kg acepromazine intramuscularly and 0.6 mg/kg xylazine intravenously, anaesthesia was induced either with 2 g thiopentone in 500 ml of a 10 per cent guaifenesin solution, given intravenously at a dose of 1 ml/kg (group TG), or with 100 mg/kg guaifenesin and 2.2 mg/kg ketamine given intravenously (group KG), or with 0.06 mg/kg midazolam, and 2.2 mg/kg ketamine given intravenously (group KM). Anaesthesia was maintained with isoflurane. The mean (sd) end tidal isoflurane concentration (per cent) needed to maintain a light surgical anaesthesia (stage III, plane 2) was significantly lower in group KM (0.91 [0.03]) than in groups TG (1.11 [0.03]) and KG (1.14 [0.03]). The mean (sd) arterial pressure (mmHg) was significantly lower in group KG (67.4 [2.07]) than in groups TC (75.6 [2.23]) and KM (81.0 [2.16]). There were no significant differences in the logarithm of the heart rate, recovery time or quality of recovery between the three induction groups. However, pronounced ataxia was observed in the horses of group KM, especially after periods of anaesthesia lasting less than 75 minutes. [less ▲]

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See detailComparison of three 15N methods to correct for microbial contamination when assessing in situ protein degradability of fresh forages
Kamoun, M.; Ammar, H.; Thewis, André ULg et al

in Journal of Animal Science (2014), 92(11), 5053-5062

The use of stable15N as a marker to determine microbial contamination in nylon bag incubation residues to estimate protein degradability was investigated. Three methods using15N were compared:15N-labeled ... [more ▼]

The use of stable15N as a marker to determine microbial contamination in nylon bag incubation residues to estimate protein degradability was investigated. Three methods using15N were compared:15N-labeled forage (dilution method, LF),15N enrichment of rumen solids-associated bacteria (SAB), and 15N enrichment of rumen liquid-associated bacteria (LAB). Herbage from forages differing in protein and fiber contents (early-cut Italian ryegrass, late-cut Italian ryegrass, and red clover) were freeze-dried and ground and then incubated in situ in the rumen of 3 steers for 3, 6, 12, 24, and 48 h using the nylon bag technique. The15N-labeled forages were obtained by fertilizing the plots where herbage was grown with15NH4 15NO3. Unlabeled forages (obtained from plots fertilized with NH4NO3) were incubated at the same time that (15NH4)2SO4 was continuously infused into the rumen of the steers, and then pellets of labeled SAB and LAB were isolated by differential centrifugation of samples of ruminal contents. The proportion of bacterial N in the incubation residues increased from 0.09 and 0.45 g bacterial N/g total N at 3 h of incubation to 0.37 and 0.85 g bacterial N/g total N at 48 h of incubation for early-cut and late-cut ryegrass, respectively. There were differences (P < 0.001) between uncorrected N degradability values and those corrected for microbial contamination with all of the methods. Apparent N degradability of the low-N, high-fiber forage (latecut ryegrass) was 0.51, whereas the corrected values were 0.85, 0.84, and 0.77 for the LF, SAB, and LAB methods, respectively. With early-cut ryegrass and red clover, the differences between uncorrected and corrected values ranged between 6% and 13%, with small differences among the labeling methods. Generally, methods using labeled forage or labeled SAB and LAB provided similar corrected degradability values. The accuracy in estimating the extent of degradation of protein in the rumen from in situ disappearance curves is improved when values are corrected for microbial contamination of the bag residue. [less ▲]

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See detailA comparison of three calcium phosphate based space fillers in sinus elevation: a study in rabbits
LAMBERT, France ULg; Léonard, Angélique ULg; LECLOUX, Geoffrey ULg et al

in The international Journal of Oral and Maxillofacial Implants (2013), 28(2), 393-402

Aim: Sub-sinusal bone regeneration procedures are reliable procedures that are known to be efficient with the use of biomaterials alone. Nevertheless, many types of biomaterials are available, and their ... [more ▼]

Aim: Sub-sinusal bone regeneration procedures are reliable procedures that are known to be efficient with the use of biomaterials alone. Nevertheless, many types of biomaterials are available, and their efficacy in terms of bone formation and resorption rate have rarely been compared. This study aimed to compare the bone formation, resorption rate, osteoconductivity and 3D volume changes of three biomaterials often used for alveolar ridge augmentation. Materials and Methods: Rabbits underwent a double sinus lift procedure using three different types of space fillers: bovine hydroxyapatite (BHA), beta calcium phosphate (ß-TCP) and biphasic calcium phosphate (BCP). Animals were sacrificed at 1 week, 5 weeks and 6 months. Samples were subjected to X-ray microtomography and histology. Qualitative analysis was performed on non-decalcified sections, and quantitative histomorphometric analysis was conducted using SEM. Volume variations of bone augmentations were calculated at different time points. Results: All three particulated biomaterials proved to be effective in promoting osteogenesis. At 6 months, biomaterial resorption rates were significantly different across the 3 groups (p<0.0001). The highest resorption rate was found with β-TCP, in which only 6.7% of the baseline particle surface remained. At 6 months, bone was in close contact with the BHA particles, constituting a composite network; in contrast, BCP particles were often surrounded by soft tissues. In each group, no significant difference in volume variations were found at the different time point. Conclusions and clinical implications: Despite the limitations of the study, the three particulated space-filling biomaterials proved to be efficient to promote osteogenesis. High resorption rates and complete replacement of the biomaterials by bone seemed to withstand intrasinusal pressure. Further investigations in humans should consider longer follow-up periods. [less ▲]

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See detailComparison of three learning methods in a patient with early Alzheimer's disease
Bier, N.; Gagnon, L.; Van der Linden, Martial et al

in Brain and Cognition (2004, October), 56(1), 116

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See detailComparison of three methods for fractionation and enrichment of low molecular weight proteins for SELDI-TOF-MS differential analysis
De Bock, Muriel ULg; De Seny, Dominique ULg; Meuwis, Marie-Alice ULg et al

in Talanta (2010), 82

In most diseases, the clinical need for serum/plasma markers has never been so crucial, not only for diagnosis, but also for the selection of the most efficient therapies, as well as exclusion of ... [more ▼]

In most diseases, the clinical need for serum/plasma markers has never been so crucial, not only for diagnosis, but also for the selection of the most efficient therapies, as well as exclusion of ineffective or toxic treatment. Due to the high sample complexity, prefractionation is essential for exploring the deep proteome and finding specific markers. In this study, three different sample preparation methods (i.e., highly abundant protein precipitation, restricted access materials (RAM) combined with IMAC chromatography and peptide ligand affinity beads) were investigated in order to select the best fractionation step for further differential proteomic experiments focusing on the LMW proteome (MW inferior to 40,000 Da). Indeed, the aim was not to cover the entire plasma/serum proteome, but to enrich potentially interesting tissue leakage proteins. These three methods were evaluated on their reproducibility, on the SELDI-TOF-MS peptide/protein peaks generated after fractionation and on the information supplied. The studied methods appeared to give complementary information and presented good reproducibility (below 20%). Peptide ligand affinity beads were found to provide efficient depletion of HMW proteins and peak enrichment in protein/peptide profiles. [less ▲]

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See detailComparison of three metrenperone dosage regimens inhibiting the 5-hydroxytryptamine-induced pulmonary dysfunction in cattle
Linden, Annick ULg; Desmecht, Daniel ULg; Amory, Hélène ULg et al

in Research in Veterinary Science (1993), 55

The effects of a 5-hydroxytryptamine (5-HT) challenge infusion on pulmonary function values were studied in healthy calves, pretreated with varying doses of metrenperone, a selective type 2 serotonergic ... [more ▼]

The effects of a 5-hydroxytryptamine (5-HT) challenge infusion on pulmonary function values were studied in healthy calves, pretreated with varying doses of metrenperone, a selective type 2 serotonergic (5-HT2) antagonist. In the first part of the experiment, the 5-HT challenge was performed 30 minutes after administration of metrenperone at a dose of 0.025 mg kg-1 (group 1, n = 8), 0.050 mg kg-1 (group 2, n = 8) and 0.100 mg kg-1 (group 3, n = 7). In the second part, the 5-HT challenge was performed 12 hours after administration of metrenperone at a dose of 0.050 mg kg-1 (group 4, n = 5) and 0.100 mg kg-1 (group 5, n = 5). None of the three doses of metrenperone influenced the 5-HT-induced ventilatory changes. For the challenge performed 30 minutes after blockade, all three doses inhibited the bronchoconstrictive process triggered by the 5-HT challenge. There was no dose-related inhibition. On the other hand, when the challenge was performed 12 hours after metrenperone, there was a dose-related inhibition with only the higher dose (0.100 mg kg-1) showing a significant efficacy in inhibiting 5-HT-induced bronchoconstriction. It is concluded that, if 5-HT is involved in the pathophysiological processes of respiratory diseases in cattle, a 5-HT2 blockade with metrenperone at a dose rate of 0.100 mg kg-1 and with a dosage interval of 12 hours should improve pulmonary function. [less ▲]

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See detailComparison of three NDT techniques for the inspection of aeronautic composite structures
Dubé, Robin; Scheed, Laurent; Lewandoswki, Jacques et al

Conference (2013, July 28)

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See detailComparison of three serological tests for the diagnosis of canine sino-nasal aspergillosis.
Billen, Frédéric ULg; Peeters, Dominique ULg; Peters, Iain et al

in Proceedings of the 18th ECVIM-CA Congress (2008, September)

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See detailComparison of three techniques for the serological diagnosis of Neospora caninum in the dog and their use for epidemiological studies
Lasri, S.; De Meerschman, F.; Rettigner, C. et al

in Veterinary Parasitology (2004), 123(1-2), 25-32

An enzyme-linked immunosorbent assay (ELISA) was developed in our laboratory and used to determine the seroprevalence of Neospora caninum in three different dog populations in Belgium: healthy dogs from ... [more ▼]

An enzyme-linked immunosorbent assay (ELISA) was developed in our laboratory and used to determine the seroprevalence of Neospora caninum in three different dog populations in Belgium: healthy dogs from cattle farms and urban dogs with or without various neurological disorders. The test was validated and compared with two other tests: an indirect fluorescent antibody test (IFAT) and a commercially available competitive enzyme-linked immunosorbent assay (C-ELISA). The study showed a good correlation between the IFAT and the ELISA developed. When the two tests were compared with the C-ELISA, moderate positive and negative agreement indices were observed. Using our ELISA and the IFAT techniques, a high prevalence was found in farm dogs. This result showed that the neurological symptoms are not usually associated with the Neospora infection. In conclusion, the ELISA developed in our laboratory could replace the IFAT for the screening of a large number of dogs' sera. (C) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailComparison of total respiratory impedance in beagle dogs and in racing saluki's
Clercx, Cécile ULg; Leriche, H.; Mc Entee, Kathleen ULg

in 4th ESVIM Meeting - Bruxelles - Belgique (1994)

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