Differential diagnosis of tachypnea in newborn calves : a field study

in In Proceedings of the World Assoc. Buiatrics Congress (1998)

Differential anti-influenza activity among allelic variants at the Sus scrofa Mx1 locus

in Journal of Interferon & Cytokine Research (2007), 27(2), 147-155

A promising way to oppose infectious challenges would be to improve the resistance of the target species through genetic selection. Theoretically, a candidate gene is available against influenza viruses ... [more ▼]

A promising way to oppose infectious challenges would be to improve the resistance of the target species through genetic selection. Theoretically, a candidate gene is available against influenza viruses since a resistance trait was fortuitously discovered in the A2G mouse strain. This trait was demonstrated to be correlated with the expression of a specific isoform of the type I interferon (IFN)-dependent protein MX, an isoform coded by a specific allele at the mouse Mx1 locus. Two allelic polymorphisms were described recently in the Sus scrofa homologous gene. In this study, the frequencies and distribution of both alleles were evaluated among European domestic pig and wild boar populations by PCR-RFLP, and the anti-influenza activity conferred by both MX1 isoforms was evaluated in vitro using transfection of Vero cells followed by flow cytometric determination of the fraction of influenza virus-infected cells among MX-producing and MX-nonproducing cell populations. A significant difference in the anti-influenza activity brought by the two MX1 isoforms was demonstrated, which suggests that a significant improvement of innate resistance of pigs by genetic selection might be feasible provided the differences found here in vitro are epidemiologically relevant in vivo. [less ▲]

Differential diagnosis and prognosis of tension-type headache

in Olesen, J.; Goadsby, P.; Ramadan, N. (Eds.) et al The Headaches (2005)

Differential diagnosis and prognosis of tension-type headache

in Olesen, J.; Goadsby, P.; Ramadan, N. (Eds.) et al The Headaches (2005)

Differential diagnosis of Alzheimer's disease with PET.

in Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine (1994), 35(3), 391-8

PET studies have demonstrated bilateral temporo-parietal hypoperfusion and hypometabolism in probable and definite Alzheimer's disease (AD), a pattern that may help differentiate AD from other dementias ... [more ▼]

PET studies have demonstrated bilateral temporo-parietal hypoperfusion and hypometabolism in probable and definite Alzheimer's disease (AD), a pattern that may help differentiate AD from other dementias. METHODS: To evaluate the diagnostic power of cerebral metabolic distribution patterns for "cortical" degenerative dementias, PET scans obtained from 129 patients referred for differential diagnosis of dementia were analyzed visually. RESULTS: Sixty-five patients had a final clinical diagnosis of probable AD. Ninety-seven percent (97%) of those had abnormal metabolic scans and 94% showed a suggestive pattern of bilateral or unilateral temporo-parietal hypometabolism (with or without frontal involvement). Hypometabolism was unilateral in 23% of patients. Five subjects with a neuropathologically proven diagnosis of Alzheimer's disease had a suggestive metabolic pattern. One of those was an early case with frontal hypometabolism exceeding temporo-parietal involvement. Two patients with Alzheimer's-type dementia had isolated bilateral frontal hypometabolism. CONCLUSIONS: This alternative metabolic pattern may correspond to a non-Alzheimer pathology occurring in 10%-20% of patients suffering from clinically probable Alzheimer's disease. Most of the patients with possible but atypical Alzheimer's-type dementia showed isolated bilateral frontal involvement. This metabolic pattern probably corresponds to different diseases, such as Pick's disease, frontal lobe dementia or progressive subcortical gliosis. [less ▲]

Differential diagnosis of cardiac murmurs in horses

in Proceedings of the 12th Moscow International Veterinary Congress (2004)

Differential diagnosis of equine systolic murmurs

in In the Proceedings of the Voorjaardagen Congress (2008)

Differential diagnosis of neurologically expressed disorders in Western European cattle

in Revue Scientifique et Technique (International Office of Epizootics) (2003), 22(1), 83-102

A classification of neurological or neurologically expressed disorders that occur in Western European cattle aged 12 month and over has been established on the basis of aetiology, frequency and conditions ... [more ▼]

A classification of neurological or neurologically expressed disorders that occur in Western European cattle aged 12 month and over has been established on the basis of aetiology, frequency and conditions of appearance, age and type of animals concerned and the main clinical signs observed. Neurologically expressed disorders have been classified according to different groups of causes: biological, non-biological and non-specific or unknown. Differential diagnosis of neurologically expressed disorders is an essential element in the clinical epidemiological surveillance of bovine spongiform encephalopathy. A growing number of aetiologies are described in the scientific literature. The identification and centralised management of neurological disorders will make it possible, one the one hand, to take account of the inherent variability in the clinical forms encountered and in the diagnostic approaches of the observers and, on the other hand, to identify new risk factors in order to control them. [less ▲]

Differential distribution of single-stranded DNA, double-stranded DNA, and RNA in adenovirus-induced intranuclear regions of HeLa cells.

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1995), 43(8), 749-59

We investigated in great detail the fine spatial distribution of nucleic acids within adenovirus-infected HeLa cells by various immunogold labeling procedures. To detect DNA, we used the in situ terminal ... [more ▼]

We investigated in great detail the fine spatial distribution of nucleic acids within adenovirus-infected HeLa cells by various immunogold labeling procedures. To detect DNA, we used the in situ terminal deoxynucleotidyl transferase-immunogold technique. In addition to the expected evident label over the condensed host chromatin and the structures containing viral double- and single-stranded DNA, label was consistently revealed over round fibrillar spots. By contrast, other virus-induced substructures, such as compact rings, crystalloids, clear amorphous inclusions, and electron-dense amorphous inclusions, displayed no significant label. Except for the viral single-stranded DNA accumulation sites, identical labeling pattern was obtained with the in situ nick-translation-immunogold method. We further labeled the sections with anti-RNA antibodies. Label was present not only over the cytoplasm and the intranuclear fibrillogranular network but also quite obviously over the compact rings and interchromatin granule clusters. None was seen over the other nuclear structures of infected cells, notably over the fibrillar spots. We suggest that these fibrillar spots might be involved in the formation of the viral, non-encapsidated, double-stranded DNA storage site. [less ▲]

Differential effect of dietary spermine on alkaline phosphatase activity in jejunum and ileum of unweaned rats

in Biochimie (2004), 86(7), 487-493

Spermine is a low molecular weight polyamine involved in the postnatal maturation of the gut. When it is administered orally to suckling rats it induces the maturation of their spleen, liver, pancreas ... [more ▼]

Spermine is a low molecular weight polyamine involved in the postnatal maturation of the gut. When it is administered orally to suckling rats it induces the maturation of their spleen, liver, pancreas, and small intestine. We showed that this polyamine modulates differently the activity of alkaline phosphatase in jejunum and ileum in suckling rat. In 14-day-old rat which had received spermine orally for 3 days, once daily, an increase of alkaline phosphatase activity in the jejunum and a decrease of this activity in the ileum was observed. Alkaline phosphatase was located at the bottom of the villus in the control jejunum and in the whole length of the villus in spermine-treated rats. On the contrary, in ileum of controls, this enzyme was present in the whole length of the villus but disappeared in the spermine-treated animals. An enzyme mass shift was observed in the small intestine after spermine administration. Spermine administration did not change the expression of genes coding for alkaline phosphatase, suggesting a post-transcriptional modification. (C) 2004 Elsevier SAS. All rights reserved. [less ▲]