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Peer Reviewed
See detailComparison of different X-ray computed tomography techniques for the quantitative characterisation of the 3D microstructure of pear fruit tissue
Verboven, Pieter; Kerckhofs, Greet ULg; Mebatsion, Hibru K. et al

in Proceedings: Busse, G., Van Hemelrijck, D., Solodov, I., Anastasopoulos, A. (Eds.) (2007)

Detailed reference viewed: 16 (3 ULg)
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See detailComparison of Eae, Tir, Esp a and Esp b Genes of Bovine and Human Attaching and Effacing Escherichia Coli by Multiplex Polymerase Chain Reaction
China, B.; Goffaux, F.; Pirson, V. et al

in FEMS Microbiology Letters (1999), 178(1), 177-82

Attaching and effacing Escherichia coli (AEEC) virulence genes include the eae, the tir, the espA and the espB genes. These genes have been sequenced from several AEEC strains. The sequences alignments ... [more ▼]

Attaching and effacing Escherichia coli (AEEC) virulence genes include the eae, the tir, the espA and the espB genes. These genes have been sequenced from several AEEC strains. The sequences alignments revealed the presence of constant and variable regions. Multiplex polymerase chain reactions were developed, in order to determine the subtype of each gene present in a particular isolate. AEEC strains isolated from calves dead of diarrhea, from healthy calves and from infected humans were compared. The same pathotypes were found in sick and healthy calves but in inverted proportion. These pathotypes were also found in human AEEC. Although, the human EHEC strains from serotype O157 possessed their own pathotype. [less ▲]

Detailed reference viewed: 17 (1 ULg)
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See detailComparison of early stages of colorectal cancer by label free proteomics
QUESADA CALVO, Florence ULg; MEUWIS, Marie-Alice ULg; Bertrand, Virginie ULg et al

in Acta Gastroenterologica (2015, February 27)

Introduction and objectives: Colorectal cancer (CRC) is the second most frequent cancer in women and the third in men. Identification of the mechanisms of progression in these early CRC stages is ... [more ▼]

Introduction and objectives: Colorectal cancer (CRC) is the second most frequent cancer in women and the third in men. Identification of the mechanisms of progression in these early CRC stages is important to develop new diagnostic and therapeutic tools. Formalin-Fixed Paraffin-Embedded (FFPE) specimens are materials that enable proteomic clinical research. Hence our aim was to address the comparison of FFPE samples from early CRC stages patients using shotgun proteomic analysis. Methods: We performed a retrospective study on 36 CRC tissue samples (pT1N0M0, n=16 and pT2N0M0, n=20) compared together and with 40 control tissue samples (20 patients with diverticulitis, using paired inflamed (DI) and healthy tissue (DH)). Each tissue slice was macrodissected to enrich in epithelial cells. We used FFPE-FASP kit (Expedeon) for sample preparation and protein digests were analyzed using 2D-nanoAquity UPLC separation online with Q-Tof Synapt HDMSTM G2 using ion mobility as additional separation. We performed protein identification and differential analysis using Progenesis QI for proteomics (Nonlinear Dynamics). Results and discussion: We selected 149 proteins differentially distributed between T1 and T2 CRC stages which were not significantly different between CRC and DH or DI. Only 30 proteins were significantly more abundant in T1 versus T2 and 119 were distributed inversely, with a minimum fold ratio of 2. Among those, ATP synthase subunit beta, Aspartate-tRNA ligase, Haptoglobin and Kininogen were identified. . Moreover, we validated Kininogen and 3 others proteins with a significant differential distribution between pT1N0M0 and pT2N0M0 stages by immunohistochemistry. Conclusion: This FFPE retrospective study comparing T1 and T2 CRC highlighted proteins already previously identified as potential CRC biomarkers. These proteins may reflect important early changes in cancer development and may help understanding early tumor progression. [less ▲]

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See detailComparison of Egg Composition and Conservation Ability in Two Belgian Local Breeds and One Commercial Strain
Moula, Nassim ULg; Antoine-Moussiaux, Nicolas ULg; Farnir, Frédéric ULg et al

in International Journal of Poultry Sciences (2009), 8(8), 768-774

The study was conducted to evaluate the egg quality of two Belgian local chicken breeds (the Ardennaise and the Famennoise) and to compare it with a commonly bred commercial strain (Lohmann brown). Two ... [more ▼]

The study was conducted to evaluate the egg quality of two Belgian local chicken breeds (the Ardennaise and the Famennoise) and to compare it with a commonly bred commercial strain (Lohmann brown). Two criteria were used to describe egg quality: egg composition (measured through yolk to albumen ratio) and its stability during conservation (measured through pH). Egg weight, size, composition as well as albumen pH were measured on 140 eggs. Measurements were performed at days 0, 7, 14 and 21 after laying. The egg weight was highest in the Lohmann strain (62.86 g), followed by the Famennoise breed (55.51 g) and lowest in the Ardennaise (50.31 g). Yolk to albumen ratio was significantly higher in the Ardennaise (0.53) when compared with the Famennoise (0.49) and Lohmann breeds (0.43). Albumen pH at laying was lowest in the Lohmann when compared with the local breeds. However, no statistical differences among the three breeds could be detected for this parameter at days 14 or 21 postlaying. The high pH in local breed eggs is often reported to be caused by a lower conservation ability. The present results suggest some physiologically higher pH at laying not resulting from defaults in conservation. As a measure of egg freshness, pH must be adapted by the various poultry breeds. [less ▲]

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See detailComparison of emergence times and quality between isoflurane and sevoflurane in rhesus macaque (Macaca mulatta) undergoing neurosurgical procedure
Bertrand, Henri ULg; Springer, Svenja; Burnside, Wesley et al

in Laboratory Animals (2017)

Volatile agents for anaesthesia are widely used for anaesthetizing laboratory primates, and isoflurane is one of the most frequently used agents. Sevoflurane has been shown to offer a more rapid recovery ... [more ▼]

Volatile agents for anaesthesia are widely used for anaesthetizing laboratory primates, and isoflurane is one of the most frequently used agents. Sevoflurane has been shown to offer a more rapid recovery than iso- flurane in a number of species, but no comparisons have been made in non-human primates. This study compared the recovery characteristics of isoflurane and sevoflurane in rhesus macaques undergoing experi- mental neurosurgery. Twelve primates (7 males and 5 females) were randomly allocated to the treatment groups. They were sedated with ketamine (10 mg/kg) and anaesthesia was induced with propofol (usually 8 mg/kg intravenously [IV]). Anaesthesia was maintained with either sevoflurane (SEVO) (2.2 0.4%) or iso- flurane (ISO) (1.2 0.2%) and alfentanil (0.2–0.5 mg/kg/min IV) for 332–592 min. Animals were mechanically ventilated. Meloxicam (0.3mg/kg) and methylprednisolone infusion (5.4mg/kg/h) were also administered. Time to extubation after cessation of anaesthesia was significantly shorter with sevoflurane (ISO: 7.0 1.8 min; SEVO: 3.6 1.5; *P 1⁄4 0.005) as was the time to the animal sitting unaided (ISO: 15.7 8.2 min; SEVO: 7.1 1.7min; *P1⁄40.004). No significant difference in the quality of recovery following isoflurane or sevoflurane anaesthesia was found. In conclusion, isoflurane and sevoflurane are both suitable volatile agents for the maintenance of general anaesthesia in rhesus macaques undergoing experimental neurosur- gical procedures. The two volatile agents presented a similar emergence quality profile, however sevoflurane anaesthesia was associated with a faster recovery, offering the possibility of conducting earlier post-operative neurological assessment. [less ▲]

Detailed reference viewed: 14 (1 ULg)
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See detailComparison of ENFA and GLM modelling for summer habitat suitability of the sperm whale in the northwestern Mediterranean Sea
Praca, Emilie; Gannier, Alexandre; Das, Krishna ULg et al

Conference (2007)

Detailed reference viewed: 31 (2 ULg)
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See detailComparison of experimental results and numerical modelling of unreinforced load-bearing masonry structures subjected to earthquake loading
Mordant, Christophe ULg; Denoël, Vincent ULg; Degée, Hervé

in Proceedings of the SECED 2015 Conference: Earthquake Risk and Engineering towards a Resilient World (2015, July)

This paper presents a comparison of experimental tests results on unreinforced load-bearing masonry structures subjected to earthquake loading with numerical predictions. First, simple walls are submitted ... [more ▼]

This paper presents a comparison of experimental tests results on unreinforced load-bearing masonry structures subjected to earthquake loading with numerical predictions. First, simple walls are submitted to cyclic and shaking table tests. Some of them include soundproofing devices in order to investigate their influence on the general behaviour in static and dynamic conditions. Additional walls with an opening and T-shaped walls are then tested under cyclic loading. The study is focused on the frame effect, the contribution of the perpendicular wall to the global strength and the efficiency of the connection. These aspects are also studied through shaking table tests on two masonry frames with T- or L-shaped piers. The main information is expressed in terms of force-displacement curves, mechanical properties and energy dissipation for the cyclic tests. The shaking table tests provide results in terms of dynamic and mechanical properties. Comparison of the results with numerical predictions is finally performed thanks to the software TREMURI. [less ▲]

Detailed reference viewed: 38 (6 ULg)
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See detailComparison of explant responses treated with leachate and leonardite sources of humic substances during in vitro rooting of woody plants.
Tahiri, Abdelghani ULg; Destain, Jacqueline ULg; Thonart, Philippe ULg et al

in Communications in Agricultural and Applied Biological Sciences (2016), 81(1), 158-165

As heterogeneous mixtures of compounds resulting from the physical, chemical and microbiological transformations of organic residues, humic substances (HS) are mostly recognized for their biostimulation ... [more ▼]

As heterogeneous mixtures of compounds resulting from the physical, chemical and microbiological transformations of organic residues, humic substances (HS) are mostly recognized for their biostimulation of plant growth that firstly involve the root development and architecture before further putative improvement of nutrients uptakes. To avoid the interferences currently reported from external origins, the successive steps of rooting have been carried out using shoots and isolated leaves of birch and alder vitro-plants. Extracts issued from landfill leachate (LHS) has been compared to a stable formulation from leonardite ("Humifirst" 12% humic acid 3% and fulvic acid) commercialized by TRADECORP company's (HHS). Chemical analysis showed that LHS source typically contain much higher N (mainly as ammonium (93%) and chloride concentration than HHS. Used at low concentration (10 ppm) during root induction/initiation phase, both HS sources may be slightly unfavorable to the root formation (21% of reduction in primary root number) of alder but not of birch. While, in root elongation phase, there is an increase in the primary root length and lateral root number. The direct effects of HS on in vitro root development vary from one species to another depending on the root treatment stage. Results showed that both explants type response are equivalent in the development of a complete rooting system. [less ▲]

Detailed reference viewed: 156 (3 ULg)
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See detailComparison of external morphological traits of newborns to inner morphological traits of the dam in the double-muscled Belgian Blue Beef breed
Coopman, F.; Gengler, Nicolas ULg; Groen, A. F. et al

in Journal of Animal Breeding & Genetics (2004), 121(2), 128-134

In the double-muscled (DM) Belgian Blue Beef (BBB) breed, caesarean section (CS) is used as a routine management tool to prevent dystocia. This practice is criticized on animal welfare grounds. With ... [more ▼]

In the double-muscled (DM) Belgian Blue Beef (BBB) breed, caesarean section (CS) is used as a routine management tool to prevent dystocia. This practice is criticized on animal welfare grounds. With unassisted (natural) births, difficulties arise because of disproportion between the sizes of the newborn and inner pelvic sizes of the dam. In this study external morphological traits of newborns are compared with inner morphological traits of the dam. Results of this study indicate that in the DM-BBB, CS is the only means to successful calving. Therefore, no calving ease scores are available to select for less dystocia in this breed. Selection for fewer CS must be achieved by focusing on lower birth weight (BW) and decreased muscular conformation at birth, both having a sufficiently high heritability. Simultaneously, pelvic sizes of the dam should be increased. It is very likely that the look of the DM-BBB will change when selecting for less dystocia. [less ▲]

Detailed reference viewed: 21 (2 ULg)
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See detailComparison of fattening performances of booars casrated or immunized against GnRF and evaluation of the vaccination efficiency
Wavreille, Jose; Boudry, Christelle ULg; Romnée, J. M. et al

in EAAP (Ed.) Book of absracts of the 62nd annual meeing of the european federation of animal science (2011, August 29)

Detailed reference viewed: 26 (3 ULg)
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See detailComparison of feeding behaviour and performance of weaned piglets fed with two types of dry feeders with integrated Drinkers
Vandenheede, Marc ULg; Laitat, Martine ULg; Desiron, Alain et al

in Proceedings of the 32nd International congress of the International Society for Applied Ethology (1998)

Detailed reference viewed: 7 (3 ULg)
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See detailComparison of FEM simulations for the incremental forming process
Henrard, Christophe ULg; Habraken, Anne ULg; Szekeres, A. et al

in Advanced Materials Research (2005), 6-8

Incremental forming is an innovative and highly flexible sheet metal forming technology for small batch production and prototyping that does not require any adapted dies or punches to form a complex shape ... [more ▼]

Incremental forming is an innovative and highly flexible sheet metal forming technology for small batch production and prototyping that does not require any adapted dies or punches to form a complex shape. The purpose of this article is to perform FEM simulations of the forming of a cone with a 50-degree wall angle by incremental forming and to investigate the influence of some crucial computational parameters on the simulation. The influence of several parameters will be discussed: the FEM code used (Abaqus or Lagamine, a code developed at the University of Liège), the mesh size, the potential simplification due to the symmetry of the part and the friction coefficient. The output is given in terms of final geometry (which depends on the springback), strain history and distribution during the deformation, as well as reaction forces. It will be shown that the deformation is localized around the tool and that the deformations constantly remain close to a plane strain state for this geometry. Moreover, the tool reaction clearly depends on the way the contact is taken into account. [less ▲]

Detailed reference viewed: 89 (4 ULg)
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See detailComparison of fermentation industrial components such as corn steep and yeast extract for lactic bacteria production
Cornelius, C.; Erpicum, T.; Jacques, Philippe ULg et al

in Mededelingen van de Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen (Rijksuniversiteit te Gent) (1996), 61/4a

Detailed reference viewed: 39 (1 ULg)
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See detailComparison of Field Transfer Methods between two meshes
Bussetta, Philippe ULg; Ponthot, Jean-Philippe ULg

in Proceedings of "IV European Conference on Computational Mechanics" (2010)

In many cases, the numerical computation of mechanical problem with Finite Element Method has to transfer some information between two different meshes. For example, if a remeshing is needed or if several ... [more ▼]

In many cases, the numerical computation of mechanical problem with Finite Element Method has to transfer some information between two different meshes. For example, if a remeshing is needed or if several meshes are used (e.g. one for a thermal problem and another one for a mechanical problem). In spite of the research on the Transfer Methods, none of them has been so far clearly established as the best. Each method has advantages and disadvantages. Many problems can happen during the field transfer, like the minimization of the numerical diffusion, the value of the field on the boundaries, etc. This paper compares on the one hand the performances of the Field Transfer Method by classical interpolation with on the other hand one using Mortar Elements. The comparison of the two methods is based on two indicators: the numerical diffusion and the evaluation of the field on the boundaries. In this paper, only the continuous fields are considered. [less ▲]

Detailed reference viewed: 72 (4 ULg)
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See detailComparison of five radioimmunoassay systems for PAG measurement: Ability to detect early pregnancy in cows
Ayad, A.; Melo de Sousa, Noelita ULg; Sulon, Joseph ULg et al

in Reproduction in Domestic Animals (2007), 42(4), 433-440

This study was conducted to describe the minimum detection limit, reproducibility, accuracy, specificity and parallelism of different pregnancy-associated glycoprotein radioimmunoassay (PAG-RIA) systems ... [more ▼]

This study was conducted to describe the minimum detection limit, reproducibility, accuracy, specificity and parallelism of different pregnancy-associated glycoprotein radioimmunoassay (PAG-RIA) systems: RIA-497, RIA-706, RIA-780, RIA-809 and RIA-Pool. Their ability to distinguish between non-pregnant and pregnant females at day 30 after artificial insemination (Al) was investigated. The antisera were raised in rabbits against different PAG preparations. All RIA systems proved to be sensitive, repeatable and accurate for measuring PAG concentrations. The dilutions of plasma samples taken at an early stage of pregnancy were found to be parallel to the standard curves. No cross-reaction was observed with different carbohydrates, either with Pregnant Marc Serum Gonadotropin (PMSG) or human Chorionic Gonadotropin (hCG). The concentrations of PAG in pregnant females at day 30 after Al were shown to be higher with the use of antisera R#706, R#780, R#809 and Pool when compared with antiserum R#497. All the RIA systems gave 100% sensitivity and negative predictive values. On the other hand, the use of antisera R#780 and R#809 resulted in lower specificity and positive predictive values. The present study clearly shows that the ability of PAG-RIA systems to diagnose pregnancy specifically at day 30 after Al can be improved by using a combination of antisera raised against different forms of PAG. [less ▲]

Detailed reference viewed: 36 (3 ULg)