Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailComparison of two FFPE preparation methods using label-free shotgun proteomics: Application to tissues of diverticulitis patients.
QUESADA CALVO, Florence ULg; Bertrand, Virginie ULg; Longuespée, Rémi ULg et al

in Journal of proteomics (2014), 112C

Formalin-fixed paraffin-embedded (FFPE) specimens of patients are useful sources of materials for clinical research and have recently gained interest for use in the discovery of clinical proteomic ... [more ▼]

Formalin-fixed paraffin-embedded (FFPE) specimens of patients are useful sources of materials for clinical research and have recently gained interest for use in the discovery of clinical proteomic biomarkers. However, the critical step in this field is the ability to obtain an efficient and repeatable extraction using the limited quantities of material available for research in hospital biobanks. This work describes the evaluation of the peptide/protein extraction using FFPE sections treated by the following two methods before shotgun proteomic analysis: a commercial solution (FFPE-FASP) (filter aided sample preparation) and an antigen retrieval-derived protocol (On Slice AR). Their efficiencies and repeatabilities are compared using data-independent differential quantitative label-free analysis. FFPE-FASP was shown to be globally better both qualitatively and quantitatively than On Slice AR. FFPE-FASP was tested on several samples, and differential analysis was used to compare the tissues of diverticulitis patients (healthy and inflammatory tissues). In this differential proteomic analysis using retrospective clinical FFPE material, FFPE-FASP was reproducible and provided a high number of confident protein identifications, highlighting potential protein biomarkers. BIOLOGICAL SIGNIFICANCE: In clinical proteomics, FFPE is an important resource for retrospective analysis and for the discovery of biomarkers. The challenge for FFPE shotgun proteomic analysis is preparation by an efficient and reproducible protocol, which includes protein extraction and digestion. In this study, we analyzed two different methods and evaluated their repeatabilities and efficiencies. We illustrated the reproducibility of the most efficient method, FFPE-FASP, by a pilot study on diverticulitis tissue and on FFPE samples amount accessible in hospital biobanks. These data showed that FFPE is suitable for use in clinical proteomics, especially when the FFPE-FASP method is combined with label-free shotgun proteomics as described in the workflow presented in this work. [less ▲]

Detailed reference viewed: 87 (29 ULg)
Full Text
Peer Reviewed
See detailComparison of two immunoassays for oxidized LDL determination
Le Goff, Caroline ULg; Haleng, Jeanine ULg; Denooz, André ULg et al

in Annales de Biologie Clinique (2009), 67(1),

Detailed reference viewed: 23 (7 ULg)
Full Text
Peer Reviewed
See detailComparison of two immunoassays for quantitative measurement of oxydized-LDL
Le Goff, Caroline ULg; Haleng, Jeanine ULg; Chapelle, Jean-Paul ULg

in Immuno-Analyse & Biologie Spécialisée [=IBS] (2009), 24

Detailed reference viewed: 14 (3 ULg)
Full Text
Peer Reviewed
See detailComparison of two inverse analysis techniques for learning deep excavation response
Hashash, Youssef; Levasseur, Séverine ULg; Osouli, Abdolreza et al

in Computers & Geotechnics (2010), 37

Performance observation is a necessary part of the design and construction process in geotechnical engineering. For deep urban excavations, empirical and numerical methods are used to predict potential ... [more ▼]

Performance observation is a necessary part of the design and construction process in geotechnical engineering. For deep urban excavations, empirical and numerical methods are used to predict potential deformations and their impacts on surrounding structures. Two inverse analysis approaches are described and compared for an excavation project in downtown Chicago. The first approach is a parameter optimization approach based on genetic algorithm (GA). GA is a stochastic global search technique for optimizing an objective function with linear or non-linear constraints. The second approach, self-learning simulations (SelfSim), is an inverse analysis technique that combines finite element method, continuously evolving material models, and field measurements. The optimization based on genetic algorithm approach identifies material properties of an existing soil model, and SelfSim approach extracts the underlying soil behavior unconstrained by a specific assumption on soil constitutive behavior. The two inverse analysis approaches capture well lateral wall deflections and maximum surface settlements. The GA optimization approach tends to overpredict surface settlements at some distance from the excavation as it is constrained by a specific form of the material constitutive model (i.e. hardening soil model); while the surface settlements computed using SelfSim approach match the observed ones due to its ability to learn small strain non-linearity of soil implied in the measured settlements. [less ▲]

Detailed reference viewed: 62 (5 ULg)
Peer Reviewed
See detailA Comparison of Two Learning and Teaching Centres in the E.U. Area : Strategies for Quality Enhancement of Teaching and Learning
Vigentini, Lorenzo; Leduc, Laurent ULg

in Nygaard, Claus; Courtney, Nigel; Bartholomew, Paul (Eds.) Quality Enhancement of University Teaching and Learning (2013)

This chapter portrays the particular realities and activities of two Centres devoted to the support and enhancement of teaching in two European Universities, respectively from Scotland and Belgium. After ... [more ▼]

This chapter portrays the particular realities and activities of two Centres devoted to the support and enhancement of teaching in two European Universities, respectively from Scotland and Belgium. After providing an overview of the conceptualization of quality used, we introduce the comparison of the strategic priorities and activities at the light of this theoretical framework. The details emerging from the description of the origins, objectives and the actions implemented affords to derive common denominators and identify key differences in the ways in which the two Universities support academic development, pedagogical innovation and the alignment to EU regulations. The comparison offers a rich overview of the potential of such units to achieve institutional strategic goals to benefit both academics and students and ultimately enhance learning and teaching. [less ▲]

Detailed reference viewed: 29 (3 ULg)
Full Text
Peer Reviewed
See detailComparison of two methods currently used to determine the interaction between cyclodextrins and drugs: phase solubility diagrams and NMR spectroscopy
Piel, Géraldine ULg; Moutard, Stephane; Perly, Bruno et al

in Journal of Drug Delivery Science and Technology (2004), 14(1, JAN-FEB), 87-91

Two methods usually used in the literature to determine stability constant values (Kc) of cyclodextrin complexes were compared: the phase solubility diagram and NMR spectroscopy. Two model drugs were used ... [more ▼]

Two methods usually used in the literature to determine stability constant values (Kc) of cyclodextrin complexes were compared: the phase solubility diagram and NMR spectroscopy. Two model drugs were used to determine limitations of both techniques: betamethasone and miconazole, with three cyclodextrins: beta-cyclodextrine (betaCD), dimethylated-betaCD (Dimeb) and trimethylated-betaCD (Trimeb). This study shows that both techniques can give the same Kc value if they are used in exactly the same conditions with well defined cyclodextrins. As a matter of fact, if the degree of substitution of cyclodextrin is not well defined (as it is often the case with Dimeb), results are biased. This study also shows that when interactions between both molecules are weak (< 1000 M-1), stability constants can not be determined by NMR due to low chemical shift variations. The limitations of the phase solubility diagram method are an oversimplification of the solubility data which can lead to large errors in the calculation of the stability constant values. Moreover, this method is time and material consuming. [less ▲]

Detailed reference viewed: 65 (7 ULg)
Full Text
Peer Reviewed
See detailA comparison of two methods for pulmonary function testing in horses with upper and lower airway obstruction
Van Erck, Emmanuelle; Duvivier, D. H.; Art, Tatiana ULg et al

Poster (1997, October 24)

Detailed reference viewed: 30 (1 ULg)
Full Text
Peer Reviewed
See detailComparison of Two Methods, UHPLC-UV and UHPLC-MS/MS, for the Quantification of Polyphenols in Cider Apple Juices
Verdu, Cindy ULg; Gatto, Julia; Freuze, Ingrid et al

in Molecules (2013), 18/9

Abstract: The aim of this study was to develop faster and more efficient phenotyping methods for in-depth genetic studies on cider apple progeny. The UHPLC chromatographic system was chosen to separate ... [more ▼]

Abstract: The aim of this study was to develop faster and more efficient phenotyping methods for in-depth genetic studies on cider apple progeny. The UHPLC chromatographic system was chosen to separate polyphenolic compounds, and quantifications were then simultaneously performed with a UV-PDA detector and an ESI-triple quadrupole mass analyzer (SRM mode). Both quantification methods were validated for 15 major compounds using two apple juice samples, on the basis of linearity, limits of detection and quantification, recovery and precision tests. The comparison between UV and SRM quantifications in 120 different samples of a cider apple progeny showed an excellent correlation for major compounds quantified with both methods. However, an overestimation was revealed for five compounds with the UV detector and the mass analyzer. Co-elution and matrix effects are discussed to explain this phenomenon. SRM methods should therefore be considered with restrictions in some cases for quantification measurements when several phenolic compounds are simultaneously quantified in complex matrices such as apple juices. For both methods, analyses were carried out over short periods of time while maintaining a high quality for the simultaneous quantification of phenolic compounds in apple juice. Each method is relevant for more in-depth genetic studies of the polyphenol content of apple juice. [less ▲]

Detailed reference viewed: 37 (16 ULg)
Peer Reviewed
See detailComparison of two novel control strategies for a closed loop micromachined tunnelling accelerometer
Gaura; E.; Kraft, Michael ULg

in Proc. 4rd Conf. on Modeling and Simulation of Microsystems (2001, March)

Detailed reference viewed: 13 (0 ULg)
Peer Reviewed
See detailComparison of two physiognomies of rupestrian fields occurring along a soil gradient
Le Stradic, Soizig ULg; Buisson, Elise; Fernandes, Geraldo Wilson

Conference (2010)

Detailed reference viewed: 25 (0 ULg)
Full Text
Peer Reviewed
See detailA Comparison of Two Spelling Brain-Computer Interfaces Based on Visual P3 and SSVEP in Locked-In Syndrome
Combaz, Adrien; Chatelle, Camille ULg; Robben, Arne et al

in PLoS ONE (2013), 8(9), 73691

Objectives: We study the applicability of a visual P3-based and a Steady State Visually Evoked Potentials (SSVEP)-based Brain-Computer Interfaces (BCIs) for mental text spelling on a cohort of patients ... [more ▼]

Objectives: We study the applicability of a visual P3-based and a Steady State Visually Evoked Potentials (SSVEP)-based Brain-Computer Interfaces (BCIs) for mental text spelling on a cohort of patients with incomplete Locked-In Syndrome (LIS). Methods: Seven patients performed repeated sessions with each BCI. We assessed BCI performance, mental workload and overall satisfaction for both systems. We also investigated the effect of the quality of life and level of motor impairment on the performance. Results: All seven patients were able to achieve an accuracy of 70% or more with the SSVEP-based BCI, compared to 3 patients with the P3-based BCI, showing a better performance with the SSVEP BCI than with the P3 BCI in the studied cohort. Moreover, the better performance of the SSVEP-based BCI was accompanied by a lower mental workload and a higher overall satisfaction. No relationship was found between BCI performance and level of motor impairment or quality of life. Conclusion:Our results show a better usability of the SSVEP-based BCI than the P3-based one for the sessions performed by the tested population of locked-in patients with respect to all the criteria considered. The study shows the advantage of developing alternative BCIs with respect to the traditional matrix-based P3 speller using different designs and signal modalities such as SSVEPs to build a faster, more accurate, less mentally demanding and more satisfying BCI by testing both types of BCIs on a convenience sample of LIS patients. [less ▲]

Detailed reference viewed: 63 (12 ULg)
Full Text
Peer Reviewed
See detailA comparison of two stretching programs for hamstring muscles: A randomized controlled assessor-blinded study
Demoulin, Christophe ULg; Wolfs, Sébastien ULg; Chevalier, Madeline et al

in Physiotherapy Theory & Practice (2016)

Detailed reference viewed: 152 (13 ULg)
Full Text
Peer Reviewed
See detailComparison of Ultra capacitors and Batteries Technologies to Optimize Hybrid Electric Vehicle Efficiency
Nzisabira, Jonathan; Louvigny, Yannick ULg; Duysinx, Pierre ULg

in Proceeding of 3rd European Symposium on Supercapacitors and Application (ESSCAP08) (2008, November)

The acceptance of Electric and Hybrid Electric Vehicle is related to their eco-efficiency, i.e. their ability to both reduce environmental impact while also providing a sufficient user satisfaction. The ... [more ▼]

The acceptance of Electric and Hybrid Electric Vehicle is related to their eco-efficiency, i.e. their ability to both reduce environmental impact while also providing a sufficient user satisfaction. The objective of this study is to provide a rationale design tool based on a multidisciplinary optimization approach to support the design of hybrid electric powertrain to simultaneously maximize user satisfaction complex criteria and minimize the Eco-score. In order to carry out the optimization problem efficiently the approach makes use of metamodeling techniques in order to save computation time in the optimization process that is driven by a Genetic Algorithm. The approach is applied to highlight the effect of different energy storage systems (batteries v.s. ultra capacitors) upon the optimized HEV design taking care of both Eco-score and User satisfaction. In the selected application that is a heavy urban bus with a mild hydrid electric powertrain, the ultra capacitors are slightly superior to NiMH batteries when emphasizing the Ecoscore criterion. [less ▲]

Detailed reference viewed: 120 (7 ULg)
Full Text
Peer Reviewed
See detailComparison of Ultra Capacitors, Hydraulic Accumulators and Batteries Technologies to Optimize Hybrid Vehicle Ecoefficiency
Nzisabira, Jonathan; Louvigny, Yannick ULg; Duysinx, Pierre ULg

in Proceeding of 2nd IEEE Int. Conference of Power Engineering, Energy, and Electric Drives (2009, March)

The main objective of vehicle powertrain hybridization is to improve the fuel consumption and environment pollutants impact (Eco-score) without decreasing the vehicle performances and other user ... [more ▼]

The main objective of vehicle powertrain hybridization is to improve the fuel consumption and environment pollutants impact (Eco-score) without decreasing the vehicle performances and other user satisfaction criteria. The Eco efficiency is a global index which accounts for both environmental impacts and user satisfaction. The Hybrid vehicles ability to overcome these two requirements depends on the optimal choice of their key mechanical, electric or hydraulic components. Some energy storage systems like ultra capacitors and hydraulic system are environmentally friendly and meet some user satisfaction criteria like daily cost, security, short charge/discharge duration and long life service. On the contrary, others like batteries have some times a environmental impact mitigate. The objective of this study is to establish a comparison between HEV (hybrid electric vehicle) and HHV(hybrid hydraulic vehicle) configuration by highlighting the effect of different energy storage systems (batteries, ultra capacitors, hydraulic system), mechanical and electric components sizes (engine, motors) upon the optimized HEV design taking care of both Eco-score and User satisfaction for different driving scenarios. The approach is formulated as a multidisciplinary optimization problem. At first, the HEV and HHV are modeled and simulated using ADVISOR (advanced vehicle simulator) with respect to several driving situations. Then emissions can be determined and the Ecoscore indicator can be calculated. User Satisfaction can be evaluated based on performance criteria extracted from ADVISOR simulation and on simple evaluation tools relying on the state-of-the art of technological information for safety, reliability and daily cost. The design problem is stated as follows: select mechanical, electric or hydraulic components (e.g. engine, motor and energy storage system sizes) to minimize the Ecoscore indicator and to maximize user satisfaction criteria subject to catalogue constraints on the choice of the components. The approach is illustrated on applications dealing with mild parallel hybrid buses. Results show that the hybrid electric buses using ultra capacitors have almost the same performances as those using batteries. However, the preferred choice would go towards super capacities because they have other appreciable properties compared to the batteries: the very high lifetime, high efficiency of charge and discharge and no polluting recyclability. Despite a smaller fuel saving than a HEV, the HHV technology has a chance to compete with HEV because of the hydraulic components low cost (industrial maturity), no pollutants recyclability due to the possible using of water as motor/pump fluid. HHV technology is a possible alternative in niche markets such as urban buses; bin-lorries or heavy urban delivery vehicles. [less ▲]

Detailed reference viewed: 339 (9 ULg)
Peer Reviewed
See detailComparison of ultrasonography and MRI in the evaluation of palmar foot pain
Van Thielen, B.; Murray, R.; De Ridder, F. et al

in ESVOT Proceedings (2008)

Detailed reference viewed: 16 (1 ULg)
Full Text
Peer Reviewed
See detailComparison of ultrasonography, bovine pregnancy specific B, and bovine pregnancy-associated glycoprotein 1 test for pregnancy detection in dairy cows
Szenci, Otto; Beckers, Jean-François ULg; Humblot, Patrice et al

in Theriogenology (1998), 50(1), 77-88

At Days 26 to 58 after AI, 138 Holstein-Friesian dairy cows were repeatedly examined by ultrasonography, using a 7.5 MHz linear-array rectal transducer. The total calving rate was 37.6% (52/138), and late ... [more ▼]

At Days 26 to 58 after AI, 138 Holstein-Friesian dairy cows were repeatedly examined by ultrasonography, using a 7.5 MHz linear-array rectal transducer. The total calving rate was 37.6% (52/138), and late embryonic mortality occurred 8.6% of the cows (12/138). On the days of ultrasound scanning, blood samples were drawn from the jugular vein for measuring the concentration of bovine pregnancy-specific protein B (bPSPB) and bovine pregnancy-associated glycoprotein 1 (bPAG 1). When compared with calving results, there were no significant differences in accurate diagnosis of pregnant cows were found between the 3 methods. However, when recognition of an embryo proper with a beating heart was used as the criterion for positive ultrasonographic diagnosis significantly fewer (P<0.001) pregnant cows were correctly identified than by the other 2 tests. When compared with the noncalving cows, significantly fewer (P<0.001) false positive diagnoses were made by the 2 ultrasonographic tests than by the PSPB and bPAG 1 tests, while significantly fewer (P<0.001) false positive diagnoses were made by the bPSPB test than by the bPAG 1 test. The accuracy of detecting nonpregnant animals by both protein tests was limited by the relatively long half-life of these proteins after calving and by early embryonic mortality. [less ▲]

Detailed reference viewed: 64 (1 ULg)
Full Text
Peer Reviewed
See detailComparison of ultrasound and pregnancy associated glycoproteins to diagnose and follow ongoing or failing pregnancy in pregnant heifers on the market
Prvanović, N; Beckers, Jean-François ULg; Sulon, J et al

in Slovenian veterinary research (2006), 43(10), 41-43

The objective of the present study was to compare transrectal ultrasonography and pregnancy associated glycoprotein (PAG) tests for determination of pregnancy in 72 Holstein-Friesian heifers imported from ... [more ▼]

The objective of the present study was to compare transrectal ultrasonography and pregnancy associated glycoprotein (PAG) tests for determination of pregnancy in 72 Holstein-Friesian heifers imported from Holland to Croatia. All heifers came to Croatia with written confirmation of pregnancy. During caranteen we preformed rectal and ultrasound exploration of heifers and simultaneously collected blood samples from each heifer. PAG concentrations were determined by a RIA immunoassay using bovine PAG 67 KDa as a tracer and standard. In our study 68 heifers were confirmed to be pregnant by both methods and 4 cows were confirmed for abortion by PAG test and nonpregnant according to ultrasound checking. Transrectal ultrasonography is accurate test for pregnancy diagnosis in pregnant heifers but it is impossible to say only on basis of ultrasound examination if heifers conceived and aborted or they failed to conceive in the first place. PAG RIA test is not only highly accurate and reliable method to distinguish between pregnant and nonpregnant heifers on the market. It is also useful to determine whether heifers were pregnant and embryo died and was absorbed during the transfer or heifers failed to conceive. It is possible because level of PAG persists in circulation after abortion or embrional death. In doubtful cases it can be used as a proof of pregnancy existence in heifers later confirmed to be nonpregnant. [less ▲]

Detailed reference viewed: 16 (0 ULg)
Full Text
Peer Reviewed
See detailCOMPARISON OF ULTRASOUND-GUIDED VS. STANDARD LANDMARK TECHNIQUES FOR TRAINING NOVICE OPERATORS IN PLACING NEEDLES INTO THE LUMBAR SUBARACHNOID SPACE OF CANINE CADAVERS
Etienne, Anne-Laure ULg; Delguste, Catherine ULg; Busoni, Valeria ULg

in Veterinary Radiology & Ultrasound (2016)

The standard technique for placing a needle into the canine lumbar subarachnoid space is primarily based on palpation of anatomic landmarks and use of probing movements of the needle, however, this ... [more ▼]

The standard technique for placing a needle into the canine lumbar subarachnoid space is primarily based on palpation of anatomic landmarks and use of probing movements of the needle, however, this technique can be challenging for novice operators. The aim of the current observational, prospective, ex vivo, feasibility study was to compare ultrasound-guided vs. standard anatomic landmark approaches for novices performing needle placement into the lumbar subarachnoid space using dog cadavers. Eight experienced operators validated the canine cadaver model as usable for training landmark and ultrasound-guided needle placement into the lumbar subarachnoid space based on realistic anatomy and tissue consistency. With informed consent, 67 final year veterinary students were prospectively enrolled in the study. Students had no prior experience in needle placement into the lumbar subarachnoid space or use of ultrasound. Each student received a short theoretical training about each technique before the trial and then attempted blind landmark-guided and ultrasound-guided techniques on randomized canine cadavers. After having performed both procedures, the operators completed a self-evaluation questionnaire about their performance and self-confidence. Total success rates for students were 48% and 77% for the landmark- and ultrasound-guided techniques, respectively. Ultrasound guidance significantly increased total success rate when compared to the landmark-guided technique and significantly reduced the number of attempts. With ultrasound guidance self-confidence was improved, without bringing any significant change in duration of the needle placement procedure. Findings indicated that use of ultrasound guidance and cadavers are feasible methods for training novice operators in needle placement into the canine lumbar subarachnoid space. [less ▲]

Detailed reference viewed: 25 (4 ULg)