Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailCytoreductive Surgery and Hyperthermic Intraperitoneal Chemotherapy for Colorectal Peritoneal Carcinomatosis : Higher Complication Rate for Oxaliplatin Compared to Mitomycin C
Rouers, A.; Laurent, S.; Detroz, Bernard ULg et al

in Acta Chirurgica Belgica (2006), 106

Peritoneal carcinomatosis (PC) from colo-rectal cancer carries a very poor prognosis with a mean and median overall survival times of 6.9 and 5.2 months. It has been proved that a locoregional therapeutic ... [more ▼]

Peritoneal carcinomatosis (PC) from colo-rectal cancer carries a very poor prognosis with a mean and median overall survival times of 6.9 and 5.2 months. It has been proved that a locoregional therapeutic approach of this disease with cytoreduction followed by hyperthermic intraperitoneal chemotherapy (HIPEC) improved survival of these patients. However, this combined treatment presents a high complication rate. Methods : 21 patients with PC of colorectal origin underwent complete cytoreduction followed by HIPEC using Mitomycin-C (13 patients) or oxaliplatin (8 patients) and the open coliseum technique. For each case the medical datas were retrospectively analysed to determine feasibility, morbidity, mortality, survival time and prognostic factors. Results : All patients presented a Sugarbaker’s Peritoneal Cancer index inferior to 15. The mean operating time was 453 minutes. After a median follow-up of 24.9 months, actuarial disease-free survival was 36.6% at 5 years. The median survival time was 34 months. The morbidity rate was 33.3% with a significant higher complication rate in the oxaliplatin group (5/8) than in the Mytomycin-C (MMC) group (2/13). One patient (4.7%) died two months after treatment with MMC (endocarditis). Conclusions : This series confirm positive impact of cytoreduction and HIPEC on PC. We obtained a moderated complications rate thanks to a high degree of selection of the patient. Oxaliplatin scheme is responsible of a higher morbidity than in MMC group. Phase III trial comparing these two drugs is needed. [less ▲]

Detailed reference viewed: 15 (3 ULg)
Full Text
Peer Reviewed
See detailCytosine Deaminase Suicide Gene Therapy for Peritoneal Carcinomatosis
Bentires-Alj, M.; Hellin, A. C.; Lechanteur, Chantal ULg et al

in Cancer Gene Therapy (2000), 7(1), 20-6

Gene therapy is a novel therapeutic approach that might soon improve the prognosis of some cancers. We investigated the feasibility of cytosine deaminase (CD) suicide gene therapy in a model of peritoneal ... [more ▼]

Gene therapy is a novel therapeutic approach that might soon improve the prognosis of some cancers. We investigated the feasibility of cytosine deaminase (CD) suicide gene therapy in a model of peritoneal carcinomatosis. DHD/K12 colorectal adenocarcinoma cells transfected in vitro with the CD gene were highly sensitive to 5-fluorocytosine (5-FC), and a bystander effect could also be observed. Treating CD+ cells with 5-FC resulted in apoptosis as detected by terminal deoxynucleotidyltransferase-mediated deoxyuridine triphosphate nick-end labeling. In vitro, several human cell lines derived from ovarian or colorectal carcinomas, as well as the rat glioblastoma 9 L cell line, responded to CD/5-FC and showed a very strong bystander effect. 5-FC treatment of peritoneal carcinomatosis generated in syngeneic BDIX rats by CD-expressing DHD/K12 cells led to a complete and prolonged response and to prolonged survival. Our study thus demonstrated the efficacy of CD suicide gene therapy for the treatment of peritoneal carcinomatosis. [less ▲]

Detailed reference viewed: 44 (1 ULg)
See detailCytoskeletal genes and idiopathic epilepsies
Lakaye, Bernard ULg; de Nijs, Laurence ULg; Leon, Christine et al

in Schwartzkroin, A. (Ed.) Encyclopedia of Basic Epilepsy Research (2009)

Detailed reference viewed: 5 (1 ULg)
Full Text
Peer Reviewed
See detailCytosolic proteins regulate alpha-synuclein dissociation from presynaptic membranes.
Wislet-Gendebien, Sabine ULg; D'Souza, Cheryl; Kawarai, Toshitaka et al

in Journal of Biological Chemistry (2006), 281(43), 32148-55

Intracellular accumulation of insoluble alpha-synuclein in Lewy bodies is a key neuropathological trait of Parkinson disease (PD). Neither the normal function of alpha-synuclein nor the biochemical ... [more ▼]

Intracellular accumulation of insoluble alpha-synuclein in Lewy bodies is a key neuropathological trait of Parkinson disease (PD). Neither the normal function of alpha-synuclein nor the biochemical mechanisms that cause its deposition are understood, although both are likely influenced by the interaction of alpha-synuclein with vesicular membranes, either for a physiological role in vesicular trafficking or as a pathological seeding mechanism that exacerbates the propensity of alpha-synuclein to self-assemble into fibrils. In addition to the alpha-helical form that is peripherally-attached to vesicles, a substantial portion of alpha-synuclein is freely diffusible in the cytoplasm. The mechanisms controlling alpha-synuclein exchange between these compartments are unknown and the possibility that chronic dysregulation of membrane-bound and soluble alpha-synuclein pools may contribute to Lewy body pathology led us to search for cellular factors that can regulate alpha-synuclein membrane interactions. Here we reveal that dissociation of membrane-bound alpha-synuclein is dependent on brain-specific cytosolic proteins and insensitive to calcium or metabolic energy. Two PD-linked mutations (A30P and A53T) significantly increase the cytosol-dependent alpha-synuclein off-rate but have no effect on cytosol-independent dissociation. These results reveal a novel mechanism by which cytosolic brain proteins modulate alpha-synuclein interactions with intracellular membranes. Importantly, our finding that alpha-synuclein dissociation is up-regulated by both familial PD mutations implicates cytosolic cofactors in disease pathogenesis and as molecular targets to influence alpha-synuclein aggregation. [less ▲]

Detailed reference viewed: 51 (4 ULg)
Full Text
Peer Reviewed
See detailCytotoxic Activity of Some Triterpenoid Saponins
Quetin-Leclercq, J.; Elias, R.; Balansard, G. et al

in Planta Medica (1992), 58

The present note deals with the cytotoxic activity of purified saponins of hedera helix and of saponins isolated from other plants ( Calendula officinalis, C. arvensis and Sapindus mukurossi with a view ... [more ▼]

The present note deals with the cytotoxic activity of purified saponins of hedera helix and of saponins isolated from other plants ( Calendula officinalis, C. arvensis and Sapindus mukurossi with a view to the detection of possible structure-activity relationships. The most active compounds are the monodesmosides which show some degree of cytotoxicity at concentrations of 10 micrograms/ml and above. Among them, alpha- and beta-hederin are the most potent substances. [less ▲]

Detailed reference viewed: 111 (0 ULg)
Full Text
Peer Reviewed
See detailCytotoxic and mitogenic activities in culture media conditioned by mouse B16 melanoma cells and 3T3 fibroblasts
Siwek, Brigitte; Wauthy, Jacques ULg; Coucke, Paul et al

in Anticancer Research (1991), 11(2), 755-759

Cytotoxic (M.W.< 1000) and mitogenic (M.W.>10000) soluble factors are released into media conditioned by pure or mixed populations of mouse 3T3 fibroblasts and B16 melanoma cells cutivated in vitro. They ... [more ▼]

Cytotoxic (M.W.< 1000) and mitogenic (M.W.>10000) soluble factors are released into media conditioned by pure or mixed populations of mouse 3T3 fibroblasts and B16 melanoma cells cutivated in vitro. They are generally more active on B16 than on fibroblasts. [less ▲]

Detailed reference viewed: 15 (1 ULg)
See detailCytotoxic aporphine alkaloids from Cassytha filiformis L
Stévigny, caroline; Gillet, Marie-Claire ULg; de Hoffmann, E et al

Poster (2001)

Detailed reference viewed: 20 (0 ULg)
Full Text
Peer Reviewed
See detailCytotoxic aporphine alkaloids from Cassytha filiformis.
Stevigny, C.; Block, S.; De Pauw, Marie-Claire ULg et al

in Planta Medica (2002), 68(11), 1042-4

Purification of a cytotoxic crude alkaloid extract of Cassytha filiformis led to the isolation of four known aporphine alkaloids: neolitsine, dicentrine, cassythine (= cassyfiline) and actinodaphnine ... [more ▼]

Purification of a cytotoxic crude alkaloid extract of Cassytha filiformis led to the isolation of four known aporphine alkaloids: neolitsine, dicentrine, cassythine (= cassyfiline) and actinodaphnine. Their structures were determined by analysis of spectroscopic data. All isolated alkaloids were tested for their cytotoxic activities on cancer and non-cancer cell lines in vitro. Neolitsine was the most active against HeLa and 3T3 cells (IC 50 :21.6 microM, and 21.4 microM, respectively). Cassythine and actinodaphnine showed the highest activity against Mel-5 (IC 50 : 24.3 microM and 25.7 microM, respectively) and HL-60 (IC 50 : 19.9 microM and 15.4 microM, respectively). This is the first report on the cytotoxic activity of C. filiformis extract and of neolitsine and cassythine. Furthermore, the complete NMR data of cassythine and actinodaphnine are given here for the first time. [less ▲]

Detailed reference viewed: 45 (1 ULg)
Full Text
Peer Reviewed
See detailCytotoxic Effects of Aroclor 1254 on Ultrastructure and Biochemical Parameters in Cultured Foetal Rat Hepatocytes
Thomé, Jean-Pierre ULg; Roelandt, L.; Goffinet, Gerhard ULg et al

in Toxicology (1995), 98(1-3), 83-94

The cytotoxicity of a commercial PCB mixture, Aroclor 1254, was assessed on cultured foetal rat hepatocytes. Under control conditions, dexamethasone stimulates immature hepatocytes to differentiate into ... [more ▼]

The cytotoxicity of a commercial PCB mixture, Aroclor 1254, was assessed on cultured foetal rat hepatocytes. Under control conditions, dexamethasone stimulates immature hepatocytes to differentiate into both hepatocytes and biliary epithelial cells. Consequently, foetal rat hepatocytes maintain, in vitro, a liver-like organization with spaces corresponding to the lumen of biliary canalicules, many mitochondria, and a well-developed rough endoplasmic reticulum (RER). This in vivo-like organization of cultured rat hepatocytes remains unchanged in medium supplemented with Aroclor 1254 at concentrations below 25 microM. In the 25-125 microM concentration range, however, PCBs severely alter some cellular organelles, notably causing important development of the RER and the appearance of cytoplasmic lacunae containing laminated concentric membrane arrays. In addition, the number of lipid droplets increases, the glycogen islets disappear, and dramatic local alterations of the mitochondrial cristae occur. In exposed and unexposed cells, the following biochemical parameters were measured: the DNA content, protein synthesis, lipid peroxidation, and urea formation. The results show that Aroclor 1254 at concentrations exceeding 25 microM (but not at lower concentrations) causes irreversible damage to cultured hepatocytes. The observed ultrastructural modifications are in good agreement with several in vivo studies on rat liver. Thus, isolated foetal rat hepatocytes have considerable potential as an alternative to whole animals for use in (eco)toxicological studies. [less ▲]

Detailed reference viewed: 10 (0 ULg)
Peer Reviewed
See detailCytotoxic interactions of 5-fluorouracil and nucleoside analogues in vitro
Li, Y-X; COUCKE, Philippe ULg; Paschoud, N et al

in Anticancer Research (1997), 17(1A), 21-27

The cytotoxic interaction of combined 5-fluorouracil (5-FU) with different nucleoside analogues was investigated in vitro on a colon (WiDr) and a breast (MCF-7) cancer cell line. Azidothymidine (AZT), 3 ... [more ▼]

The cytotoxic interaction of combined 5-fluorouracil (5-FU) with different nucleoside analogues was investigated in vitro on a colon (WiDr) and a breast (MCF-7) cancer cell line. Azidothymidine (AZT), 3'-deoxy-2', 3'-didehydrothymidine (D4T), 5-iododeoxyuridine (IdUrd) and 2',3'-dideoxycytidine (DDC) were tested at different concentrations (5-600 microM) as modulators of 5-FU. The experimental endpoints were cellular viability and cell cycle distribution. The combination of 5-FU and AZT or D4T yielded supra-additive cytotoxic effects in both cell lines at all concentrations. On WiDr, IdUrd at high concentrations of 50 and 100 microM showed a supra-additive effect whereas at low concentrations (5, 10 and 20 microM) the effect was antagonistic. 5-FU combined with IdUrd produced a synergistic effect on MCF-7 cells at all concentrations. DDC antagonised the toxic effect of 5-FU on the WiDr cell line. In WiDr cells, a significant increase in the overall S-phase was observed 48 and 72 hours after exposure to D4T, AZT and DDC at the low concentration of 10 microM. On the contrary, this accumulation in S-phase was not present in MCF-7 cells. The combined effect of 5-FU and nucleoside analogues in vitro is dependent on the type and concentration of nucleosides and the cell-line tested. AZT, D4T and IdUrd are more likely to be subjected to more intensive in vitro and in vivo research as far as modulation of 5-FU toxicity is concerned. [less ▲]

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailCytotoxic triterpenoid saponins from the roots of Cephalaria gigantea
Tabatadze, Nino; Elias, Riad; Faure, Robert et al

in Chemical & Pharmaceutical Bulletin (2007), 55(1), 102-105

Three new oleanane-type saponins, giganteosides L (1)(I), M (2) and N (3) along with eight known ones were isolated from the roots of Cephalaria gigantea. Their structures were established as 3-O-[-D ... [more ▼]

Three new oleanane-type saponins, giganteosides L (1)(I), M (2) and N (3) along with eight known ones were isolated from the roots of Cephalaria gigantea. Their structures were established as 3-O-[-D-galactopyranosyl-(12)--D-glucuronopyranosyl]-28-O-[-D-glucopyranosyl-(16)--D-glucopyranosyl]-oleanolic acid, 3-O-[-D-galactopyranosyl-(12)--D-glucuronopyranosyl]-28-O-[-D-glucopyranosyl-(16)--D-glucopyranosyl]-hederagenin, 3-O-[-L-rhamnopyranosyl-(12)--D-glucuronopyranosyl]-28-O-[-D-glucopyranosyl-(16)--D-glucopyranosyl]-hederagenin, resp., by means of spectroscopic methods (1D and 2D NMR, HR-ESI-MS). Cytotoxic activity of monodesmosides was investigated in vitro using three cancer cell lines, namely, human non pigmented melanoma MEL-5 and human leukemia HL-60. Giganteosides D (4) and E (5) showed antiproliferative effect on human cell lines with IC50 values in the range 3.15-7.5 M. [less ▲]

Detailed reference viewed: 29 (2 ULg)
Full Text
Peer Reviewed
See detailCytotoxicity towards human endothelial cells, induced by neutrophil myeloperoxidase: protection by ceftazidime.
Mathy, Marianne ULg; Deby, Ginette ULg; Deby, C. et al

in Mediators of Inflammation (1995), 4(6), 437-43

We investigated the effects of the antibiotic ceftazidime (CAZ) on the cytolytic action of the neutrophil myeloperoxidase-hydrogen peroxide-chloride anion system (MPO/H(2)O(2)/Cl(-)). In this system ... [more ▼]

We investigated the effects of the antibiotic ceftazidime (CAZ) on the cytolytic action of the neutrophil myeloperoxidase-hydrogen peroxide-chloride anion system (MPO/H(2)O(2)/Cl(-)). In this system, myeloperoxidase catalyses the conversion of H(2)O(2) and CI(-) to the cytotoxic agent HOCl. Stimulated neutrophils can release MPO into the extracellular environment and then may cause tissue injury through direct endothelial cells lysis. We showed that human umbilical vein endothelial cells (HUVEC) were capable of taking up active MPO. In presence of H(2)O(2) (10(-4) M), this uptake was accompanied by cell lysis. The cytolysis was estimated by the release of (51)Cr from HUVEC and expressed as an index of cytotoxicity (IC). Dose dependent protection was obtained for CAZ concentrations ranging from 10(-5) to 10(-3) M;this can be attributed to inactivation of HOCl by the drug. This protection is comparable to that obtained with methionine and histidine, both of which are known to neutralize HOCl. This protection by CAZ could also be attributed to inactivation of H(2)O(2), but when cytolysis was achieved with H(2)O(2) or O(2) (-) generating enzymatic systems, no protection by CAZ was observed. Moreover, the peroxidation activity of MPO (action on H(2)O(2)) was not affected by CAZ, while CAZ prevented the chlorination activity of MPO (chlorination of monochlorodimedon). So, we concluded that CAZ acts via HOCl inactivation. These antioxidant properties of CAZ may be clinically useful in pathological situations where excessive activation of neutrophils occurs, such as in sepsis. [less ▲]

Detailed reference viewed: 31 (4 ULg)
Full Text
Peer Reviewed
See detailCZC-GC miniaturized analysis of POPs in 20 µL blood
L'Homme, Benjamin ULg; Focant, Jean-François ULg

Conference (2013, May)

Detailed reference viewed: 23 (6 ULg)
Peer Reviewed
See detailCZE separation of basic and acidic drug by use of cyclodextrin additives
Bechet, I.; Fillet, Marianne ULg; Fotsing, Lucas ULg et al

Poster (1995)

Detailed reference viewed: 8 (2 ULg)
Full Text
Peer Reviewed
See detailCzochralski crystal growth, thermal conductivity, and magnetic properties of Pr(x)La(1- x)AlO3, where x = 1, 0.75, 0.55, 0.40, 0
Turczynski, S.; Orlinski, K.; Pawlak, D. A. et al

in Crystal Growth & Design (2011), 11(4), 1091-1097

Crystals of Pr(x)La(1-x)AlO3 solid solution have been grown by the Czochralski method for the first time, to the best of our knowledge. Crystals with high praseodymium concentration tend to grow spirally ... [more ▼]

Crystals of Pr(x)La(1-x)AlO3 solid solution have been grown by the Czochralski method for the first time, to the best of our knowledge. Crystals with high praseodymium concentration tend to grow spirally. The color of the crystals changes with chemical composition. X-ray diffraction shows an increase of the lattice constants with an increase in lanthanum ion concentration. The thermal conductivity has been investigated in the temperature range from 5 to 300 K. Completely different behavior of thermal conductivity is observed at low temperatures for the investigated crystals, whereas it remains roughly constant at high temperatures. The values of magnetic susceptibility lie within the expected range that could be expected for a paramagnetic material. (Figure Presented) © 2011 American Chemical Society. [less ▲]

Detailed reference viewed: 29 (6 ULg)
See detail'D’abord sa manière étoit gottique'. L'œuvre peint de Bernard van Orley jusqu'au début des années 1520
Galand, Alexandre ULg

Doctoral thesis (2011)

Les années 1510 et 1520 apparaissent comme un moment crucial pour l’introduction de la Renaissance dans les anciens Pays-Bas. Or, cette période coïncide avec la première période d’activité de Bernard van ... [more ▼]

Les années 1510 et 1520 apparaissent comme un moment crucial pour l’introduction de la Renaissance dans les anciens Pays-Bas. Or, cette période coïncide avec la première période d’activité de Bernard van Orley, un témoin majeur de cette époque de transition. Jusqu’à présent, la recherche a surtout envisagé l’artiste par le prisme de ses magnifiques tapisseries. De la fin de la première décennie du 16e siècle à 1521 où il signe le monumental Polyptyque de Job et de Lazare (Bruxelles, MRBAB/KMSKB, Inv. 1822), l’artiste a pourtant élaboré un œuvre peint de plus en plus perméable aux formes en provenance de la péninsule italienne. Ce changement de perspective a-t-il entraîné des différences importantes en termes de pratiques d’atelier ? Comment peuvent être caractérisées de manière précise les œuvres constituant le noyau dur d’un catalogue aux frontières mouvantes ? En quoi les documents d’archives peuvent-ils donner un nouvel éclairage sur l’activité de l’artiste ? Ces questions sont au centre d’un travail qui se donne pour ambition de renouveler la vision de la peinture de Bernard van Orley, domaine jusqu’à aujourd’hui négligé. [less ▲]

Detailed reference viewed: 81 (13 ULg)