Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Peer Reviewed
See detailCrystallization and preliminary X-ray data for parvalbumin IIIf of Opsanus tau.
Hamoir, G.; Dideberg, O.; Charlier, Paulette ULg

in Journal of Molecular Biology (1981), 153(2), 487-9

Detailed reference viewed: 17 (1 ULg)
Peer Reviewed
See detailCrystallization and preliminary X-ray data for the exocellular beta-lactamase of Bacillus licheniformis 749/C.
Dideberg, O.; Libert, M.; Frère, Jean-Marie ULg et al

in Journal of Molecular Biology (1985), 181(1), 145-6

The exocellular beta-lactamase from Bacillus licheniformis 749/C has been crystallized from polyethylene glycol solution at pH 5.5. An X-ray examination of the monoclinic crystals shows the space group is ... [more ▼]

The exocellular beta-lactamase from Bacillus licheniformis 749/C has been crystallized from polyethylene glycol solution at pH 5.5. An X-ray examination of the monoclinic crystals shows the space group is P21, with unit cell dimensions a = 66.77 A, b = 93.77 A, c = 43.57 A and beta = 104.5 degrees. The asymmetric unit consists of two molecules of 28,500 Mr each. The crystals are suitable for structure analysis to at least 2 A resolution. [less ▲]

Detailed reference viewed: 21 (1 ULg)
Full Text
Peer Reviewed
See detailCrystallization and preliminary X-ray diffraction studies of a-amylase from the antarctic psychrophile Alteromonas haloplanctis A23
Aghajari, N.; Feller, Georges ULg; Gerday, Charles ULg et al

in Protein Science : A Publication of the Protein Society (1996), 5(10), 2128-2129

A cold-active alpha-amylase was purified from culture supernatants of the antarctic psychrophile Alteromonas haloplanctis A23 grown at 4 degrees C. In order to contribute to the understanding of the ... [more ▼]

A cold-active alpha-amylase was purified from culture supernatants of the antarctic psychrophile Alteromonas haloplanctis A23 grown at 4 degrees C. In order to contribute to the understanding of the molecular basis of cold adaptations, crystallographic studies of this cold-adapted enzyme have been initiated because a three-dimensional structure of a mesophilic counterpart, pig pancreatic alpha-amylase, already exists. alpha-Amylase from A. haloplanctis, which shares 53% sequence identity with pig pancreatic alpha-amylase, has been crystallized and data to 1.85 A have been collected. The space group is found to be C222(1) with a = 71.40 A, b = 138.88 A, and c = 115.66 A. Until now, a three-dimensional structure of a psychrophilic enzyme is lacking. [less ▲]

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailCrystallization and X-ray diffraction study of the Streptomyces K15 penicillin-binding DD-transpeptidase.
Englebert, S.; Charlier, Paulette ULg; Fonze, E. et al

in Journal of Molecular Biology (1994), 241(2), 295-7

The 262 amino acid residue long DD-transpeptidase/penicillin-binding protein of Streptomyces K15 has been crystallized at room temperature by using the hanging drop vapour diffusion technique. The ... [more ▼]

The 262 amino acid residue long DD-transpeptidase/penicillin-binding protein of Streptomyces K15 has been crystallized at room temperature by using the hanging drop vapour diffusion technique. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit cell parameters a = 46.4 A, b = 54.1 A and c = 108.3 A. They contain one protein molecule per asymmetric unit and diffract to about 1.9 A. X-ray data have been collected to 2.0 A from a native crystal. The previously published amino acid sequence of the protein has been corrected at positions 71, 72, 113, 114 and 156. [less ▲]

Detailed reference viewed: 10 (0 ULg)
Full Text
Peer Reviewed
See detailCrystallization behaviour of binary fat blends containing shea stearin as hard fat
Danthine, Sabine ULg; Delatte, S; Blecker, Christophe ULg et al

Conference (2014, September 15)

Detailed reference viewed: 6 (0 ULg)
Full Text
Peer Reviewed
See detailCrystallization of a genetically engineered water-soluble primary penicillin target enzyme. The high molecular mass PBP2x of Streptococcus pneumoniae.
Charlier, Paulette ULg; Buisson, G.; Dideberg, O. et al

in Journal of Molecular Biology (1993), 232(3), 1007-9

A genetically engineered water-soluble derivative of PBP2x of Streptococcus pneumoniae has been produced, purified and crystallized in a form suitable for X-ray diffraction analysis. The best crystals ... [more ▼]

A genetically engineered water-soluble derivative of PBP2x of Streptococcus pneumoniae has been produced, purified and crystallized in a form suitable for X-ray diffraction analysis. The best crystals have been grown at 15 degrees C, from solutions containing 8% polyethylene glycol 10,000 at pH values ranging from 3.9 to 6.0. These crystals diffract to a resolution of 3.5 A and have a space group P6(1)22 (or enantiomorph) with unit cell dimensions of a = b = 162.2 A, c = 171.8 A, alpha = beta = 90 degrees, gamma = 120 degrees. The molecular mass and cell dimensions suggest that there is one molecule of enzyme per asymmetric unit. The breakdown of a chromogenic cephalosporin derivative diffused into a crystal reveals clearly that the enzyme is active in the crystalline state. [less ▲]

Detailed reference viewed: 26 (0 ULg)
Full Text
Peer Reviewed
See detailCrystallization of ornithine acetyltransferase from yeast by counter-diffusion and preliminary X-ray study
Maes, D.; Crabeel, M.; Van de Weerdt, Cécile ULg et al

in Acta Crystallographica Section F-Structural Biology and Crystallization Communications (2006), 62(Part 12), 1294-1297

A study is presented on the crystallization of ornithine acetyltransferase from yeast, which catalyzes the fifth step in microbial arginine synthesis. The use of the counter-diffusion technique removes ... [more ▼]

A study is presented on the crystallization of ornithine acetyltransferase from yeast, which catalyzes the fifth step in microbial arginine synthesis. The use of the counter-diffusion technique removes the disorder present in one dimension in crystals grown by either the batch or hanging-drop techniques. This makes the difference between useless crystals and crystals that allow successful determination of the structure of the protein. The crystals belong to space group P4, with unit-cell parameters a = b = 66.98, c = 427.09 angstrom, and a data set was collected to 2.76 angstrom. [less ▲]

Detailed reference viewed: 21 (1 ULg)
Peer Reviewed
See detailCrystallization of vitreous high-T-c superconducting oxide through laser zone melting method.
Ausloos, Marcel ULg; Bougrine, Hassan ULg; Cloots, Rudi ULg et al

in Institute of Physics Conference Series (1995), 148

We synthesized Bi2Sr2CaCu2O8-y ceramics through the vitreous route. After insertion in epoxy resin, we exposed the materials to a CO2 laser beam. Various intensities and sweeping conditions were examined ... [more ▼]

We synthesized Bi2Sr2CaCu2O8-y ceramics through the vitreous route. After insertion in epoxy resin, we exposed the materials to a CO2 laser beam. Various intensities and sweeping conditions were examined. We observed the samples in high resolution polarized light microscopy and with electron scanning microscopy. Crystallization zones could be detected. A systematic analysis gave the correlation between the behavior of the material and its synthesis conditions. Electrical properties were measured through micro-electrode deposited on the surface at various locations. No superconductivity was found. The optimization of such a process and potential applications will be discussed. [less ▲]

Detailed reference viewed: 17 (5 ULg)
Full Text
Peer Reviewed
See detailCrystallization sequence and magma chamber processes in the ferrobasaltic Sept Iles layered intrusion, Canada
Namur, Olivier ULg; Charlier, Bernard ULg; Toplis, Michael et al

in Journal of Petrology (2010), 51

Detailed reference viewed: 65 (7 ULg)
Full Text
Peer Reviewed
See detailCrystallization, X-ray characterization and selenomethionine phasing of Mlc1p bound to IQ motifs from myosin V
Terrak, Mohammed ULg; Otterbein, L. R.; Wu, G. et al

in Acta Crystallographica Section D-Biological Crystallography (2002), 58(Pt 10 Pt 2), 1882-5

Mlc1p is a calmodulin-like protein from the budding yeast Saccharomyces cerevisiae, where it has been identified as a subunit of a class V myosin, Myo2p, and a binding partner of an IQGAP-like protein ... [more ▼]

Mlc1p is a calmodulin-like protein from the budding yeast Saccharomyces cerevisiae, where it has been identified as a subunit of a class V myosin, Myo2p, and a binding partner of an IQGAP-like protein, Iqg1p. Through its interactions with these two proteins, Mlc1p plays a role in polarized growth and cytokinesis. Mlc1p has been crystallized in complexes with four different IQ target motifs from the neck region of Myo2p: IQ2, IQ3, IQ4 and IQ2-IQ3 (referred to as IQ2,3). Electron-density maps for two of the complexes (Mlc1p-IQ4 and Mlc1p-IQ2,3) were obtained from multiple anomalous dispersion (MAD) experiments based on selenomethionine derivatives. The other two structures (Mlc1p-IQ2 and Mlc1p-IQ3) were determined by molecular replacement using the partially refined structure of Mlc1p-IQ2,3 as a search model. [less ▲]

Detailed reference viewed: 20 (4 ULg)
Full Text
Peer Reviewed
See detailCrystallographic analysis of family 11 endo-beta-1,4-xylanase Xyl1 from Streptomyces sp. S38.
Wouters, J.; Georis, J.; Engher, D. et al

in Acta Crystallographica Section D-Biological Crystallography (2001), 57(Pt 12), 1813-9

Family 11 endo-beta-1,4-xylanases degrade xylan, the main constituent of plant hemicelluloses, and have many potential uses in biotechnology. The structure of Xyl1, a family 11 endo-xylanase from ... [more ▼]

Family 11 endo-beta-1,4-xylanases degrade xylan, the main constituent of plant hemicelluloses, and have many potential uses in biotechnology. The structure of Xyl1, a family 11 endo-xylanase from Streptomyces sp. S38, has been solved. The protein crystallized from ammonium sulfate in the trigonal space group P321, with unit-cell parameters a = b = 71.49, c = 130.30 A, gamma = 120.0 degrees. The structure was solved at 2.0 A by X-ray crystallography using the molecular-replacement method and refined to a final R factor of 18.5% (R(free) = 26.9%). Xyl1 has the overall fold characteristic of family 11 xylanases, with two highly twisted beta-sheets defining a long cleft containing the two catalytic residues Glu87 and Glu177. [less ▲]

Detailed reference viewed: 21 (9 ULg)
Peer Reviewed
See detailCrystallographic data for a penicillin receptor : exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61
Knox, James R.; DeLucia, Mary L.; Murthy, N. S. et al

in Journal of Molecular Biology (1979), 127(2), 217-218

A pencillin-sensitive enzyme, the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61, has been crystallized from polyethylene glycol (Mr = 6000 to 7500) solution at pH 7•6. X-ray ... [more ▼]

A pencillin-sensitive enzyme, the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61, has been crystallized from polyethylene glycol (Mr = 6000 to 7500) solution at pH 7•6. X-ray examination of the orthorhombic crystals shows the space group is P212121, with unit cell dimensions a = 51•1 Å, B = 67•4 Å, and C = 102•9 Å. With four molecules of molecular weight 38,000, the Å3/dalton ratio for the cell is 2•33. The crystals are stable to irradiation for 75 hours and are suitable for structure analysis to at least 2•4 Å resolution. The radius of gyration of the molecule in solution at pH 6.8 is 20.8 Å. [less ▲]

Detailed reference viewed: 12 (0 ULg)
Peer Reviewed
See detailCrystallographic data for the beta-lactamase from Enterobacter cloacae P99.
Charlier, Paulette ULg; Dideberg, O.; Frère, Jean-Marie ULg et al

in Journal of Molecular Biology (1983), 171(2), 237-8

The beta-lactamase from Enterobacter cloacae P99 has been crystallized from polyethylene glycol solution at pH 7. X-ray examination of the orthorhombic crystals shows the space group is P2(1)2(1)2 with ... [more ▼]

The beta-lactamase from Enterobacter cloacae P99 has been crystallized from polyethylene glycol solution at pH 7. X-ray examination of the orthorhombic crystals shows the space group is P2(1)2(1)2 with unit cell dimensions a = 77.4 A, b = 69.4 A, and c = 63.6 A. There is one molecule of molecular weight 39,000 in the asymmetric unit. [less ▲]

Detailed reference viewed: 26 (1 ULg)
Full Text
Peer Reviewed
See detailCrystallographic data for the DD-carboxypeptidase-endopeptidase of low penicillin sensitivity excreted by Streptomyces albus g
Dideberg, Otto; Frère, Jean-Marie ULg; Ghuysen, Jean-Marie ULg

in Journal of Molecular Biology (1979), 129(4), 677-679

The DD-carboxypeptidase-endopeptidase of low penicillin sensitivity that is excreted by Streptomyces albus G has been crystallized from a polyethylene glycol (Mr 6000 to 7500) solution at pH 8.0. X-ray ... [more ▼]

The DD-carboxypeptidase-endopeptidase of low penicillin sensitivity that is excreted by Streptomyces albus G has been crystallized from a polyethylene glycol (Mr 6000 to 7500) solution at pH 8.0. X-ray examination of the prismatic crystals shows that the space group is P21 with unit cell dimensions a = 51.1 Å, B = 49.7 Å, C = 38.7 Å, β = 100.6 ° and one molecule in the asymmetric unit. A crystal suspension made in 50 mM -Tris • HCl buffer (pH 8.0) supplemented with 5 mM-MgCl2 and 16% (w/v) polyethylene glycol exhibits enzyme activity on the substrate Ac2-L-Lys-D-Ala-D-Ala. [less ▲]

Detailed reference viewed: 3 (0 ULg)
Full Text
Peer Reviewed
See detailCrystallographic mapping of β-lactams bound to a D-alanyl-D-alanine peptidase target enzyme
Kelly, Judith A.; Knox, James R.; Zhao, Haiching C. et al

in Journal of Molecular Biology (1989), 209(2), 281-295

X-ray crystallography has been used to examine the binding of three members of the beta-lactam family of antibiotics to the D-alanyl-D-alanine peptidase from Streptomyces R61, a target of penicillins ... [more ▼]

X-ray crystallography has been used to examine the binding of three members of the beta-lactam family of antibiotics to the D-alanyl-D-alanine peptidase from Streptomyces R61, a target of penicillins. Cephalosporin C, the monobactam analog of penicillin G and (2,3)-alpha-methylene benzylpenicillin have been mapped at 2.3 A resolution in the form of acyl-enzyme complexes bound to serine 62. On the basis of the positions of these inhibitors, the binding of a tripeptide substrate for the enzyme, L-lysyl-D-alanyl-D-alanine, has been modeled in the active site. The binding of both inhibitors and substrate is facilitated by hydrogen-bonding interactions with a conserved beta-strand (297-303), which is antiparallel to the beta-lactam's acylamide linkage or the substrate's peptide bond. The active site is similar to that in beta-lactamases. [less ▲]

Detailed reference viewed: 12 (0 ULg)
Peer Reviewed
See detailCrystallographic studies of low penicillin-sensitive enzyme excreted by Streptomyces albus G [proceedings]
Dideberg, O.; Charlier, Paulette ULg

in Archives Internationales de Physiologie et de Biochimie (1979), 87(5), 1045

Detailed reference viewed: 18 (1 ULg)
Full Text
Peer Reviewed
See detailThe CSDP Olympiad from a Scientific Point of View
Paile, Sylvain ULg

in Zambas, Symeon (Ed.) The 1st Common Security and Defence Policy Olympiad (2013)

Detailed reference viewed: 21 (1 ULg)
See detailCSL TV chambers Updating
Grodent, Christophe ULg; franco

Conference (2006)

Detailed reference viewed: 15 (4 ULg)
See detailCSR Performance Measurements: Motivations, Tools and Survey Results
Crutzen, Nathalie ULg

Scientific conference (2010, December 02)

Detailed reference viewed: 62 (6 ULg)
Full Text
Peer Reviewed
See detailCT FEATURES OF A LACRIMAL GLAND TUMOR IN A DOG
Etienne, Anne-Laure ULg; Grauwels, Magda ULg; Storms, Goedele ULg et al

Poster (2014, August)

Background: In the dog, neoplasms of the lacrimal gland are rare. Lacrimal cysts were also reported. Computed tomography (CT) scan findings of these lacrimal gland abnormalities have not been described in ... [more ▼]

Background: In the dog, neoplasms of the lacrimal gland are rare. Lacrimal cysts were also reported. Computed tomography (CT) scan findings of these lacrimal gland abnormalities have not been described in dogs. Purpose: The present report describes clinical and CT features of a lacrimal gland tumor in a dog. Methods: A 3,5 year old female intact Leonberger with a good general health status was presented with a progressive swelling at the level of the supero-temporal area of the left upper eyelid and of the bulbar conjunctiva since 3 weeks. Ocular examination revealed a slight enophthalmia and a ventronasal strabismus, the presence of a firm mass in the upper eyelid which was anteriorly well circumscribed and extending posteriorly between the globe and the orbital ligament. Findings: A CT scan pre- and post- contrast studies of the head were performed. A 3 cm hypoattenuating (+/- 10 HU) ovoid mass was observed dorsolateral to the indented left globe. This mass was well circumscribed by an unevenly thick irregular soft tissue- attenuating wall (50HU), which was strongly contrast-enhanced (150HU) and measured about 2 to 10mm in thickness. The left globe was displaced caudoventromedially. No adjacent bone lesion, or foreign body was observed. No normal left lacrimal gland was observed. These findings were suggestive of an abscess or an orbital tumor with a necrotic/cystic center; a lacrimal cyst was considered less likely because of the unevenness and the thickness of the wall. Complete surgical ablation was done by a modified lateral orbitotomy. Histopathology revealed a mixed benign tumor of the lacrimal gland and a lymphocytic necrotic adenitis. Six months postoperatively no recurrence is noted and the tear production is 15mm/min. Conclusion: The lacrimal gland is located dorsolateral to the globe and produces tears. In human medicine, a wide range of lacrimal gland pathologies were described and assessed by computed imaging techniques. Pleomorphic adenomas (mixed benign tumor) could reveal irregular bone erosion and could undergo malignant transformation. In dogs, lacrimal cysts were described as thin walled structures.4 Only few articles reported lacrimal gland tumor. To the authors knowledge, CT findings of a lacrimal gland tumor have not been described previously. In conclusion, lacrimal gland tumor should be included in the differential diagnosis of a firm ovoid cystic/necrotic mass dorsolateral to the orbit. [less ▲]

Detailed reference viewed: 10 (0 ULg)