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See detailCrural artery bypass with the autogenous greater saphenous vein
Van Damme, Hendrik ULg; Zhang, Lihong ULg; Baguet, E. et al

in European Journal of Vascular and Endovascular Surgery : The Official Journal of the European Society for Vascular Surgery (2003), 26(6), 635-642

Objective. To evaluate the long-term outcome of greater saphenous vein (GSV) infrapopliteal revascularisation in a single centre over a 10 year period. Material and methods. Fourty-one variables relating ... [more ▼]

Objective. To evaluate the long-term outcome of greater saphenous vein (GSV) infrapopliteal revascularisation in a single centre over a 10 year period. Material and methods. Fourty-one variables relating to a consecutive series of 90 crural artery GSV(76% in situ) bypasses in 81 patients (1990-2000) were analysed. The mean age of the 47 men and 34 women was 70 years. Limb-threatening ischaemia was present in 96% of cases, claudication in four patients. In 18 patients, surgery was 'redo'. Results. The perioperative mortality was 3% (n = 3). Patient survival was 54% at 4 years. Independent risk factors affecting survival were chronic renal insufficiency (p = 0.04), hypertension (p = 0.02), and ischaemic heart disease (p = 0.01). Four bypasses thrombosed within 30 days. Three of them could be successfully reopened. Mean follow-up was 39 months. The primary patency rate at 4 years was 80%. Chronic renal insufficiency revealed to be the single independent risk factor for graft thrombosis (p = 0.03, RR = 12.4). The 4-year limb salvage rate was 88%. No independent risk factor affecting the limb salvage could be identified. Conclusion. Crural artery revascularisation is a valuable option for the management of limb threatening infrapopliteal arterial occlusive disease. [less ▲]

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See detailCrural or pedal artery revascularisation for limb salvage: is it justified
Van Damme, Hendrik ULg

in Acta Chirurgica Belgica (2004), 104(2), 148-157

With the ageing of population, the incidence of limb-threatening ischemia increases. In chronic critical limb ischemia, peripheral arterial occlusive disease almost always involves infrainguinal and ... [more ▼]

With the ageing of population, the incidence of limb-threatening ischemia increases. In chronic critical limb ischemia, peripheral arterial occlusive disease almost always involves infrainguinal and infragenicular vessels. Fortunately, recent advances in vascular surgery made arterial reconstruction of crural and pedal vessels possible. Should crural or pedal bypass surgery be offered to these frail, polyvascular patients, or is primary amputation a preferable treatment option in case of advanced limb-threatening ischemia? In order to answer this controversial question, the author analysed recent literature data on the feasibility and durability of infrapopliteal bypasses. The quality of life was also considered as an outcome measure. Finally, the cost-effectiveness of both treatment modalities (limb-saving distal bypass versus primary amputation) was assessed. [less ▲]

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See detailThe crustacean scavenger guild in Antarctic shelf, bathyal and abyssal communities
De Broyer, Claude; Nyssen, Fabienne ULg; Dauby, Patrick ULg

in Deep-Sea Research Part II, Topical Studies in Oceanography (2004), 51(14-16), 1733-1752

Peracarid crustaceans form a significant part of the macrobenthic community that is responsible for scavenging on large food falls onto the sea floor. Although several studies are available about ... [more ▼]

Peracarid crustaceans form a significant part of the macrobenthic community that is responsible for scavenging on large food falls onto the sea floor. Although several studies are available about scavengers from tropical and temperate seas, very little information has been published about such species living in Antarctic waters, particularly at greater depths. The present paper is based on a collection of 31 baited trap sets deployed in the Weddell Sea, Scotia Sea, and off the South Shetland Islands, and presents results on the geographical and bathymetric distribution of the different taxa and on the eco-functional role of scavengers. <br /> <br />Some 68,000 peracarid crustaceans from 62 species were collected. About 98% of individuals belonged to the amphipod superfamily Lysianassoidea, and 2% to the isopod family Cirolanidae. Of these species, 31, including 26 lysianassoids (1400 individuals), were collected deeper than 1000 m. <br /> <br />High species richness was discerned for the eastern Weddell Sea shelf compared with other Antarctic areas. The Antarctic slope also seems to be richer in species than other areas investigated in the world, while in the abyss, scavenger species richness appears to be lower in Antarctica. A richness gradient was thus observed from the shelf to the deep. For amphipods, a number of species extend their distribution from the shelf to the slope and only one to the abyssal zone. <br /> <br />Amphipod species showed degrees of adaptation to necrophagy. The functional adaptations of the mandible and the storage function of the gut are discussed. Feeding experiments conducted on lysianassoid species collected at great depths and maintained in aquaria showed a mean feeding rate of about 1.4–4.1% dry body weight day−1, which is consistent with data obtained from other species. [less ▲]

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See detailThe crustal tongue melting model and the origin of massive anorthosites
Duchesne, Jean-Clair ULg; Liégeois, Jean-Paul ULg; Vander Auwera, Jacqueline ULg et al

in Terra Nova (1999), 11

Recent detailed field studies in several anorthosite complexes have shown that anorthosites are frequently linked with weakness zones in the crust which favour their emplacement at mid-crust levels ... [more ▼]

Recent detailed field studies in several anorthosite complexes have shown that anorthosites are frequently linked with weakness zones in the crust which favour their emplacement at mid-crust levels. Recent experimental data have also shown that the parent magma compositions of various anorthosite massifs lie on thermal highs in the relevant phase diagrams, indicating that these magmas cannot be derived by fractionation of peridotitic mantle melts but rather are produced by melting of gabbronoritic sources. In the Sveconorwegian province terrane boundaries have been traced in deep seismic profiles to Moho offsets or to tongues of lower crustal material underthrust to depths of 40-50 km. We therefore suggest that the parent magmas of anorthosite massifs are produced by melting of gabbronoritic rocks from the lower crust that had been thrust into the mantle by collision of terranes. [less ▲]

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See detailCryo-optical test of the PLANCK reflectors
Roose, Stéphane ULg; Cucchiaro, Antonio ULg

Conference (2006, March 20)

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See detailCryo-optical testing of large aspheric reflectors operating in the sub mm range
Roose, Stéphane ULg; Houbrechts, Yvette ULg; Mazzoli, Alexandra ULg et al

in Zhang, Y.; Jiang, W.; Cho, M. (Eds.) Proceedings of the 2nd SPIE symposium on Advanced Optical Manufacturing and Testing Technologies (2005, August)

The cryo-optical testing of the PLANCK primary reflector (elliptical off-axis CFRP reflector of 1550 mm x 1890 mm) is one of the major issue in the payload development program. It is requested to measure ... [more ▼]

The cryo-optical testing of the PLANCK primary reflector (elliptical off-axis CFRP reflector of 1550 mm x 1890 mm) is one of the major issue in the payload development program. It is requested to measure the changes of the Surface Figure Error (SFE) with respect to the best ellipsoid, between 293 K and 50 K, with a 1 μm RMS accuracy. To achieve this, Infra Red interferometry has been used and a dedicated thermo mechanical set-up has been constructed. This paper summarises the test activities, the test methods and results on the PLANCK Primary Reflector - Flight Model (PRFM) achieved in FOCAL 6.5 at Centre Spatial de Liege (CSL). Here, the Wave Front Error (WFE) will be considered, the SFE can be derived from the WFE measurement. After a brief introduction, the first part deals with the general test description. The thermo-elastic deformations will be addressed: the surface deformation in the medium frequency range (spatial wavelength down to 60 mm) and core-cell dimpling. [less ▲]

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See detailCryogenic and Thermal Control for Vacuum Facilities Development in KARI
Guiot, Marc ULg; Jamotton, Pierre ULg; Grodent, Christophe ULg et al

in CURRAN (Ed.) 26th Aerospace Testing Seminar 2011: Los Angeles, California, USA, 29 - 31 March 2011 (2011, March 29)

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See detailCryogenic Zone Compression for the Measurement of Dioxins
Focant, Jean-François ULg; Patterson Jr

Scientific conference (2010, April)

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See detailCryogenic Zone Compression for the Measurement of Dioxins in Human Serum at Attogram Level by GCxGC-IDHRMS.
Patterson Jr; Welch; Turner et al

Conference (2008, June)

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See detailCryopréservation d’ovocytes et d’embryons par congélation ou vitrification dans le cadre de l’assistance médicale à la procréation
Vanderzwalmen, Pierre ULg; Ectors, Fabien ULg; Papras, Y et al

in Poncelet, Christophe; Sifer, Christophe (Eds.) Physiologie, pathologie et thérapie de la reproduction chez l’humain (2011)

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See detailCryopreservation des embryons humains par vitrification.
Vanderzwalmen, Pierre ULg; Zech, Nicolas; Greindl, A.-J. et al

in Gynécologie Obstétrique & Fertilité (2006), 34(9), 760-9

Vitrification is a cryopreservation strategy where cells are converted into a glass-like amorphous solid which is free of any crystalline structure. Such process is achieved by a combination of high ... [more ▼]

Vitrification is a cryopreservation strategy where cells are converted into a glass-like amorphous solid which is free of any crystalline structure. Such process is achieved by a combination of high concentration of cryoprotectant and an extremely high cooling rate. In the last years, survival rates of up to 80% after thawing and pregnancy rates of almost 30% could be achieved after transfer of vitrified embryos at the zygote, cleavage, morula and blastocyst stages. Also deliveries of healthy babies have been reported numerous times. To this day, a limited interest in this technique can be noted. The explanation may lye in the apprehension of many ART units regarding exposure of embryos to high concentrations of cryoprotectants and storage in non sterile conditions. The aim of the first part of this article, is to analyse if such fears are justified on the basis that vitrification mimics conditions already in use for many years in slow-cooling procedures where cells are plunged into liquid nitrogen at around -30 degrees C and secondly since storage of embryos are now possible in high aseptic conditions. In the second part, results on survival after thawing, pregnancy rates and baby take home rates of vitrified embryos will be presented and the problems associated with vitrification of blastocysts will be discussed. [less ▲]

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See detailCryopreservation For The Elimination Of Cucumber Mosaic And Banana Streak Viruses From Banana (Musa Spp.)
Helliot, Bertrand; Panis, B.; Poumay, Y. et al

in Plant Cell Reports (2002), 20(12), 1117-1122

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See detailCryopreservation of embryos : a way to reduce the number of housed animals and the genetic drift.
Remy, Benoît ULg; Ectors, Fabien ULg; Drion, Pierre ULg

Poster (2014, January 27)

The GIGA Mouse facility platform has recently improved its mouse line cryopreservation technique. The method of embryo cryopreservation by rapid cooling also called aseptic vitrification has been selected ... [more ▼]

The GIGA Mouse facility platform has recently improved its mouse line cryopreservation technique. The method of embryo cryopreservation by rapid cooling also called aseptic vitrification has been selected. Vitrification media, key steps and timing have been optimized and validated. After a first partial exposition of the embryos to cryoprotective solutions, they are immersed in a vitrifying mixture of penetrating and non-penetrating cryoprotectants for a short time. The straw containing the embryos is immediately sealed before to be plunged in LN2, resulting in a brutal solidification in which crystallization does not have time to occur. [less ▲]

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See detailCryopreservation of Radopholus similis, a tropical plant-parasitic nematode
Elsen, Annemie; Ferrandis Vallterra, Salvador ULg; Van Wauwe, Tom et al

in Cryobiology (2007), 55(2), 148-157

For obligate plant-parasitic nematodes, cryopreservation has advantages over the usual preservation methods on whole plants or axenic culture systems, because the latter two are labourious and time and ... [more ▼]

For obligate plant-parasitic nematodes, cryopreservation has advantages over the usual preservation methods on whole plants or axenic culture systems, because the latter two are labourious and time and space consuming. In addition, cross contamination among different isolates can occur easily. Moreover, specific genetic studies require maintenance of the original population. The nematode under investigation, Radopholus similis, is a plant-parasitic nematode from the humid tropics. Therefore, any treatment at low temperatures is likely to add extra stress to the nematode, making the development of a cryopreservation protocol extremely difficult. In this paper, we describe experiments to achieve a successful cryopreservation protocol for the tropical nematode R. similis using vitrification solution-based methods based on a well defined mixture of cryoprotectants in combination with ultra-rapid cooling and thawing rates. A two-step treatment was used consisting of an incubation in glycerol followed by the application of a vitrifying mixture of methanol, glycerol and glucose. After cryopreservation, the athogenicity of the nematodes was not altered, since they could infect and reproduce on carrot discs after recovery in the Ringer solution. The cryopreservation method described can be used for routine cryopreservation of R. similis lines from different origins. [less ▲]

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See detailCryoscopie des solides de l’organisme. Procédés et résultats
Fredericq, Léon ULg

in Bulletin de l'Académie Royale de Médecine de Belgique (1902), (novembre),

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See detailCryoscopy: a novel enhancing method of in vivo skin imaging.
Nikkels, Arjen ULg; Pierard, Gérald ULg

in Skin Research & Technology (2007), 13(4), 377-84

BACKGROUND: It is a common observation that superficial freezing of normal skin and skin tumors may create a transient superficial whitening effect. In this respect, cryoscopy refers to the direct ... [more ▼]

BACKGROUND: It is a common observation that superficial freezing of normal skin and skin tumors may create a transient superficial whitening effect. In this respect, cryoscopy refers to the direct observation by dermoscopy, with or without digital recording, of the visual alterations of the frozen tissues. AIMS: To define the optimal method of cryoscopy and to describe the cryoscopy patterns of normal skin and selected skin lesions. MATERIALS AND METHODS: The influence of (a) different cryogenic sources [solid carbon dioxide (-78.5 degrees C), liquid nitrogen (N(2), -196 degrees C), and a mixture of dimethyl ether and propane (-57 degrees C)], (b) various application methods (spraying, cotton chill tips, copper plate), and (c) freezing time was assessed with regard to clinical feasability, visualization quality, and persistance time of the whitening effect. Cryoscopy patterns of normal skin, callosities and of histologically proven seborrheic keratoses, verrucous hamartomas, molluscum contagiosum, keratoacanthomas, viral warts, condylomas, actinic keratoses, dermatofibromas, skin tags, basal cell carcinomas, angiomas, and melanocytic naevi were assessed. RESULTS: The cryoscopy images of skin highlighted the skin lines. They appeared similar regardless of the freezing source and the application method. The aspects differed according to the nature of the lesions. The cotton chill tip method provided a longer whitening period compared with the other cold sources, both in normal and lesional skin. Hence, it represented the most convenient way for performing digital recording cryoscopy. On normal skin, cryoapplication was limited to about 1.5 s due to pain, resulting in whitening times ranging from 6 to 9 s, which was too short for easy digital recording. On all studied skin tumors, a 10-s N(2) freezing time was not experienced as painful, and blanching time persisted for 20-34 s, allowing easy digital recording. The whitening time was longer with increasing freezing time on both normal and lesional skin. Every single examined normal skin site and all the skin lesions showed a strong whitening effect, except heavily cornified structures, including some keratoses, callosities, and viral warts. Increased contrast of the skin surface texture was observed in almost every studied lesion. CONCLUSION: The N(2) cotton chill tip technique appeared to be the most convenient technique for cryoscopy and provided longer whitening periods compared with the other freezing sources. Pain prevented its use on normal skin, but a series of exophytic skin lesions was conveniently accessible to cryoscopy. The differences in whitening periods of various epidermal components resulted in increased visual contrast, creating typical cryoscopy images for the different exophytic skin tumors. Cryoscopy represents a novel in vivo skin imaging technique that is rapid, non-invasive, cost-effective, and easily performed. It shows both investigative and diagnostic potentials. It is remarkable that cryoscopy pictures closely resemble those yielded by skin capacitance imaging. [less ▲]

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