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See detailContribution à la conservation de la flore cupro-cobalticole endémique du Katanga (R.D.C.) : Conservation en banque de graines et régénération d'espèces métallicoles
Boisson, Sylvain ULg

Master's dissertation (2011)

Metallophytes are plants that represent the biodiversity of metal-rich soils. Some of them are able to accumulate high metal concentrations: metal hyperaccumulators plants. In Katanga (RD Congo), 32 ... [more ▼]

Metallophytes are plants that represent the biodiversity of metal-rich soils. Some of them are able to accumulate high metal concentrations: metal hyperaccumulators plants. In Katanga (RD Congo), 32 species have been identified as copper-cobalt endemic but mining activities are still a threat to outcrop ecosystems. We must characterize native environmental parameters (realized niche) and the full range of environmental conditions in culture (fundamental niche) to conserve, preserve and restore these ecosystems. Six representative species of the natural copper gradient were selected after soil-species data analyses (Crepidorhopalon perennis, Crotalaria cobalticola, Gladiolus ledoctei, Haumaniastrum robertii, Lopholaena deltombei and Sopubia mannii). The study determined their behavior (germination, survival) and their development (vegetative measures) on one group of natural soil (from three copper gradients of Fungurume V hill), two groups of artificially contaminated soils with incremental doses (copper sulfate and copper hydroxycarbonate: 500, 2000 and 5000 mg.kg-1) and one uncontaminated soil (control) by two types of plant establishment (direct seeding and transplant from Petri dishes). The results depend on species habitat and show that four of these species can grow on uncontaminated soil in the early stages of growth. In addition, the seed bank (in Faculty of Agronomy - University of Lubumbashi) management (cleaning, updating and germination tests of accessions) was maintained using standard methods. The results of germination tests demonstrated the effectiveness of some conservation methods and helped to provide recommendations for the methodology. [less ▲]

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See detailContribution à la désinfection de l'eau par photosensibilisation avec des extraits de plantes
Sunda Makuba, Teddy ULg

Doctoral thesis (2012)

La problématique de l’accès à l’eau par les populations des pays en développement constitue toujours de nos jours un défi important à relever pour contribuer à un développement durable tout en ... [more ▼]

La problématique de l’accès à l’eau par les populations des pays en développement constitue toujours de nos jours un défi important à relever pour contribuer à un développement durable tout en garantissant des conditions sanitaires satisfaisantes. Malgré les Objectifs du Millénaire pour le Développement (OMD), de nombreux pays souffrent encore actuellement d’un manque d’accès à l’eau. En République Démocratique du Congo, seuls 26% de la population a accès à l’eau. Au-delà de la satisfaction des besoins humains assurés par la fourniture de la quantité d’eau nécessaire, il s’agit aussi de veiller à garantir sa bonne qualité, particulièrement la qualité microbiologique. Parmi les divers procédés de désinfection de l’eau au niveau familial, la présente contribution a pour objectif l’amélioration de la méthode de désinfection solaire par application du processus de photosensibilisation. En présence de lumière, un photosensibilisateur génère dans l’eau l’oxygène singulet très réactif conduisant à la destruction des microorganismes. Certaines plantes utilisées dans la pharmacopée traditionnelle pour soigner les infections microbiennes et parasitaires sont capables d’initier cette réaction de photosensibilisation. Dans un premier temps, parmi une dizaine de plantes analysées, nous avons mis en évidence l’activité photosensibilisante d’extraits non alcaloïdiques de Cassia alata, Cassia occidentalis, Carica papaya, Phyllanthus niruri et Coleus kilimandschari. L’inactivation des coliformes fécaux a été remarquée après trois heures d’ensoleillement, celle-ci se manifestant après une heure d’exposition lorsque le milieu est saturé en oxygène. Tout en étant libéré de la contrainte liée à la saturation du milieu en oxygène, l’étude des huiles essentielles extraites de plantes de la famille des Rutacées a montré une inhibition complète des coliformes fécaux présents dans l’eau après deux heures d’ensoleillement pour Citrus reticulata et Citrus limonum et une heure d’ensoleillement pour Citrus bergamia. L’examen de cette dernière huile en spectrométrie de masse a mis en évidence la présence de méthoxy-5 psoralène (MOP-5), responsable de l’activité photosensibilisatrice. Utilisé en solution dans l’eau, le MOP-5 a confirmé son rôle de photosensibilisateur et son efficacité désinfectante avec un abattement de 4 unités logarithmiques pour les Coliformes fécaux après une heure d’exposition et de 3 unités logarithmiques pour les Entérocoques fécaux après seulement 5 minutes d’exposition. La fixation du MOP-5 sur un support solide a permis de récupérer le photosensibilisateur après usage tout en conservant son pouvoir désinfectant supérieur à la forme libre. Le dispositif MOP-5/polystyrène a permis d’observer une inhibition complète des Coliformes fécaux après seulement 6 minutes d’exposition et deux minutes pour les Entérocoques fécaux. Cette recherche constitue un premier travail exploratoire qui ouvre la voie vers un nouveau processus de désinfection de l’eau par photosensibilisation à partir d’extraits de plantes. Mais il conviendra de préciser les modalités pratiques d’application afin de rendre ce processus opérationnel. [less ▲]

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See detailContribution à la détermination d’impacts économiques de la fertilisation d’un ferralsol par l’extrait aqueux de cendres de Cynodon dactylon à Kisangani, R.D.Congo
Bolakonga Ilye, Antoine Bily ULg; Moango Manga, Adrien; Natdanga Lele et al

in Annales de l’Institut Facultaire des Sciences Agronomiques de Yangambi (2007), 1

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See detailContribution à la détermination du profil en COVs des effluents gazeux de séchage industriel de levain
Eloundou Mballa, Pierre Patrick ULg; Fauconnier, Marie-Laure ULg

Poster (2013, July 02)

During the industrial drying of sourdough, there is an evaporation of a part of the volatile compounds and those generated during the process. These compounds are evacuated with waste and lost for the ... [more ▼]

During the industrial drying of sourdough, there is an evaporation of a part of the volatile compounds and those generated during the process. These compounds are evacuated with waste and lost for the industry. A complete characterization of the aromatic profile would open ways towards a co-valuation of some of these COVS. [less ▲]

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See detailContribution à la gestion intégrée des eaux et des sols à travers l'application du contrat de rivière Sourou au Burkina Faso
Rosillon, Françis ULg; Bado-Sama, H.

in La GIRE décryptée, éléments de renforcement de la GIRE en Haïti etdans les PED (2010)

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See detailContribution à la gestion intégrée des eaux et des sols à travers l'application du contrat de rivière Sourou au Burkina Faso.
Rosillon, Françis ULg

Conference (2007, November)

Inspired from the experience of river contract in Wallonia (Belgium), the implementation of a first river contract has been initiated in a West-African country, Burkina Faso, since 2003. In the program of ... [more ▼]

Inspired from the experience of river contract in Wallonia (Belgium), the implementation of a first river contract has been initiated in a West-African country, Burkina Faso, since 2003. In the program of actions approved by the river committees in December 2005, the protection of water resources and grounds was taken into account. The first realized actions contributed to slow down the degradation of the ecosystems by recreating a space buffer between the river and the cultivated zones. A first project of restoration of the gallery forest alongside the stream was implemented to fight against desertification. This project arouses a strong mobilization of the local actors and demonstrates that such a contractual tool of participative management can make an interesting contribution in favour of an integrated management of waters and grounds. In a river contract, these two resources are associated in a common reflection which transcends still too often sector-based policies. [less ▲]

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See detailContribution à la maîtrise des contaminations des plantes potagères par les éléments traces métalliques
Liénard, Amandine ULg

Master's dissertation (2009)

The set of problems resulting from metallic trace elements in vegetable gardens is increasingly in the heart of the question, evidently as much as a concern for public health than in terms of sustainable ... [more ▼]

The set of problems resulting from metallic trace elements in vegetable gardens is increasingly in the heart of the question, evidently as much as a concern for public health than in terms of sustainable environmental management. Throughout this work, I aimed at studying particular aspects of metallic trace contaminations, mainly their dispersal across the soil and soil-plant transfers. Firstly, a toposequential study focused on the source of contamination was performed in order to allow the description of the relation between the distance from the source of contamination and the distribution of the metallic trace elements. It was observed that as far the distance from the source of contaminations is as low the concentrations of traces elements are. Secondly, two distinct experiments were performed to assess the soil-plant transfers. The first one consisted in a culture on a range of contaminated soils of two vegetables varieties (i.e. lettuce and endive). As a result, it was shown that the two varieties accumulated similarly the trace elements. The aim of the second experiment was to study the relationship of different organic matters on the phytoavailability of the traces elements. It was performed by culturing Spinacia oleracea on three contaminated soils where three different organic amendments (manure cow, manure cow-hen-horse, and compost) were added with four different concentrations. Results based on soils and S. oleracea metallic trace elements concentrations were compared to standards in application for the contaminations in trace elements in the soils and to the foodstuff standards fixing the concentrations limits of cadmium and lead in the vegetable-leafs. Finally, it was observed that it was the “manure of cow” amendment used at a rate of 120g/kg of soil that represented best way to avoid large transfers of metallic trace elements into the different parts of the plant susceptible to be eaten. [less ▲]

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See detailContribution à la mise au point d'un dispositif de culture associée cotonnier-niébé dans la région de Gandajika au Zaïre
Delhove, G.; Mergeai, Guy ULg; Mulamba, K.

in Mémoires de la Société Royale d'Entomologie de Belgique (1992), (35), 375-378

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See detailContribution à la mise au point d'une méthode de lutte biologique contre la maladie des pourritures de la couronne de la banane
Lassois, Ludivine ULg

Master's dissertation (2003)

The antagonist activity of two yeast strains (Pichia anomala K strain and Candida oleophila O strain) against the parasitic complex responsible for crowns rot of banana has been evaluated. Their ... [more ▼]

The antagonist activity of two yeast strains (Pichia anomala K strain and Candida oleophila O strain) against the parasitic complex responsible for crowns rot of banana has been evaluated. Their efficiency has been tested on three separated fungi : Colletotrichum musae, Fusarium moiliforme, Cephalosporium sp. and on a parasitic complex formed by the association of these three fungi. Antagonist effects have been observed both on the two first fungi and on the complex. Strain O added at 108 ufc/ml on crowns which were previously inoculated with the complex showed the highest protection (56,0 %). This protection was superior to those observed against the fungi inoculated separately. The influence of the time between the addition of the O strain (108 ufc/ml) and the inoculation of the pathogens on the level of protection has also been measured. It appeared that protection by the O strain was remarkably reinforced when the strain was added 24 hours before the complex (57,0 %). This, in comparison with the additions performed 15 minutes or 3 hours after the pathogens inoculation. The utilisation of the O strain at 108 ufc/ml has been combined with the utilisation of 50μm polybags. These latter allow the formation of a modified atmosphere during the length of the bunches transfer. The protection level was at 61,0% when both methods were combined whereas protection by the yeast alone was of 41,0%. A strict correlation between the severity of the symptoms and the protection by the yeast has been highlighted. When the severity of the disease increases, protection by the yeast decreases. [less ▲]

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See detailContribution à la mise au point de la cDNA-AFLP sur bananes
Lassois, Ludivine ULg

Master of advanced studies dissertation (2005)

Obtaining ARN of quality and in sufficient quantity is an essential stage in the realization of cDNA-AFLP. The effectiveness of several protocols of RNA extraction was evaluated on peel and pulp of green ... [more ▼]

Obtaining ARN of quality and in sufficient quantity is an essential stage in the realization of cDNA-AFLP. The effectiveness of several protocols of RNA extraction was evaluated on peel and pulp of green and yellow bananas. On peel and pulp of green bananas, 4 protocols were tested and two of them allowed obtaining RNA of good quality and in sufficient quantity. They acts of the protocol described by Liu and al. (1998) and that proposed by the CIRAD (not published) and which is a modified version of Wan and Wilkins (1994). However, new tests must be realized in order to choose more adapted in the case of the extractions on peel and pulp of green bananas. Seven protocols were tested and compared on peel and pulp of yellow bananas. Among those, the protocols describes by Liu and al. (1998) and that proposed by the CIRAD (not published) gave the best results. [less ▲]

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See detailContribution à la mise en place d'un système d'évaluation génétique des verrats Piétrain en croisement en Wallonie
Dufrasne, Marie ULg

Master's dissertation (2009)

The aim of this study was to contribute to the development of the genetic evaluation system for Piétrain boars in Walloon Region. These boars were evaluated on growth, carcass quality and feed intake ... [more ▼]

The aim of this study was to contribute to the development of the genetic evaluation system for Piétrain boars in Walloon Region. These boars were evaluated on growth, carcass quality and feed intake performances recorded on their crossbred progeny. The genetic evaluation for carcass quality was realized with a database of 60 546 data recorded during on-farm testing. The traits analysed were backfat thickness (BF), loin muscle depth (LMD) and meat percentage (%meat), measured on live animals by ultrasounds with the Piglog 105. The model developed was a multitrait animal model with random regression using linear splines. Fixed effects were sex, contemporary groups and heterozygosity. Random effects were permanent environment, additive genetic and residual effect. The variance components have been estimated by REML and Gibbs sampling methods. Heritability from 150 to 300 days was 0.56 to 0.75 for BF, 0.66 to 0.47 for LMD and 0.55 to 0.69 for %meat. Genetic correlations from 150 to 300 days were 0.1 to -0.05 between BF and LMD, -0.90 to -0.93 between BF and %meat, and 0.38 to 0.41 between LMD and %meat. According to the study of residuals, it seems that the model developed fits well the data. However, it would be interesting to review the variance components obtained for LMD. Moreover the position and number of knots for linear splines should be refined. Furthermore data provided by slaughterhouse will be incorporated into the genetic evaluation of carcass quality. Then this evaluation should be more accurate and comprehensive. [less ▲]

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See detailContribution à la mission CoRoT et à la recherche d'exoplanètes par la méthode des transits photométriques
Gillon, Michaël ULg

Doctoral thesis (2006)

In this thesis, we present our contributions to the CoRoT mission and to the search for exoplanets with the photometric transit method. We developped a spectroscopic analysis method, APASS, which allows ... [more ▼]

In this thesis, we present our contributions to the CoRoT mission and to the search for exoplanets with the photometric transit method. We developped a spectroscopic analysis method, APASS, which allows to obtain a precise determination of atmospheric physical parameters and abundances for stars with an unnegligeable rotational speed. We present APASS and the high-precision results we obtained for main target stars of the asteroseismological part of the CoRoT mission. Our results are in agreement with those obtained by other authors using different analysis methods, while beeing more precise. Furthermore, they show that the use of a spectroscopic analysis method based on equivalent widths measurements by gaussian or Voigt profiles fitting should be avoided for stars whose rotational velocity is significantly higher then the Sun’s, and that a method using synthetic spectra fitting for which the convolution is a free parameter should be prefered. Next, we investigate the potential of extrasolar planet transit surveys. We show that CoRoT has a very high potential for the detection of close-in giant planets, better than the one of the KEPLER mission. This latter reveals to be better for the detection of terrestrial planets. Nevertheless, CoRoT could detect a few giant telluric planets of short orbital period, provided that this kind of object is frequent in our galaxy. For ground-based surveys, our results based on the use of large telescopes and wide-field cameras seem to indicate that an important potential is also available from the ground. These results are nevertheless less reliable, mainly because of the lack of knowledge about the influence of systematic effects, and additional investigations using actual data will be necessary. We present then the deconvolution method MCS and the way we adapted it to planetary transit photometry. We present the results we obtained after analysis of actual data for OGLE-TR-123 (VLT/FORS2 data) and OGLE-TR-132 (VLT/FORS2 and NTT/SUSI2 data). These results are very encouraging and demonstrate the high potential of MCS for this kind of high-precision analysis. Finally, we present the future improvements we intend to install to obtain a still higher level of precision and to increase the speed of analysis. [less ▲]

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See detailContribution à la modélisation du séchage du maïs en lit-fixe
Janas, Sébastien ULg

Doctoral thesis (2012)

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