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See detailComplex I Mitochondrial Dysfunction in HDAC5-depleted Cancer Cells Induces Glucose-dependent Metabolic Reprogramming
Hendrick, Elodie ULg; Peixoto, Paul ULg; Polese, Catherine ULg et al

Poster (2014, February 01)

Introduction : Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure ... [more ▼]

Introduction : Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure. Broad-spectrum inhibitors of these enzymes such as SAHA can inhibit tumor growth both in vitro and in vivo and are currently used as anti-cancer agents in clinic. For many years, we are investigating the specific role of individual HDAC members in cancer biology and we have recently demonstrated that specific depletion of HDAC5 using siRNA technology reduced cancer cells proliferation and survival1. Aims : The goal of this study is to further understand the molecular mechanisms of action of HDAC5 in cancer cells. Methods and results : Screening transcriptomic study demonstrated that HDAC5 depletion induces a down-regulation of NDUFB5, a subunit of the complex I of the mitochondrial respiratory chain through modulation of mRNA stability. HDAC5 depletion-induced NDUFB5 downregulation causes a significant increase of ROS production and induces uncoupled mitochondrial respiration. In addition, this HDAC5 depletion-induced mitochondrial dysfunction provokes metabolic adaptation associated with increased importance of glucose. Indeed, interference with glucose supply in HDAC5-depleted cancer cells significantly increases apoptotic cell death suggesting that glucose deprivation might be combined to HDAC5 inhibition as a therapeutic strategy to kill cancer cells. Conclusions : Our study demonstrated for the first time that specific HDAC5 inhibition induces alteration of NDUFB5 gene expression by altering mRNA stability and provides insight into a valuable experimental strategy for manipulation of specific HDAC5 inhibition and glucose metabolism in therapy against cancer. 1.Peixoto, P. et al. HDAC5 is required for maintenance of pericentric heterochromatin, and controls cell-cycle progression and survival of human cancer cells. Cell death and differentiation, 2012; 1-14. [less ▲]

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See detailComplex I Mitochondrial Dysfunction in HDAC5-depleted Cancer Cells Induces Glucose-dependent Metabolic Reprogramming
Hendrick, Elodie ULg; Peixoto, Paul ULg; Matheus, Nicolas ULg et al

Poster (2014, January 27)

Introduction : Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure ... [more ▼]

Introduction : Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure. Broad-spectrum inhibitors of these enzymes such as SAHA can inhibit tumor growth both in vitro and in vivo and are currently used as anti-cancer agents in clinic. For many years, we are investigating the specific role of individual HDAC members in cancer biology and we have recently demonstrated that specific depletion of HDAC5 using siRNA technology reduced cancer cells proliferation and survival1. Aims : The goal of this study is to further understand the molecular mechanisms of action of HDAC5 in cancer cells. Methods and results : Screening transcriptomic study demonstrated that HDAC5 depletion induces a down-regulation of NDUFB5, a subunit of the complex I of the mitochondrial respiratory chain through modulation of mRNA stability. HDAC5 depletion-induced NDUFB5 downregulation causes a significant increase of ROS production and induces uncoupled mitochondrial respiration. In addition, this HDAC5 depletion-induced mitochondrial dysfunction provokes metabolic adaptation associated with increased importance of glucose. Indeed, interference with glucose supply in HDAC5-depleted cancer cells significantly increases apoptotic cell death suggesting that glucose deprivation might be combined to HDAC5 inhibition as a therapeutic strategy to kill cancer cells. Conclusions : Our study demonstrated for the first time that specific HDAC5 inhibition induces alteration of NDUFB5 gene expression by altering mRNA stability and provides insight into a valuable experimental strategy for manipulation of specific HDAC5 inhibition and glucose metabolism in therapy against cancer. 1.Peixoto, P. et al. HDAC5 is required for maintenance of pericentric heterochromatin, and controls cell-cycle progression and survival of human cancer cells. Cell death and differentiation, 2012; 1-14. [less ▲]

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See detailThe complex injuries produced by percutaneous injection of fluids under very high pressure
RADERMECKER, Régis ULg; Thiry, A.; Beaujean, M.

in Journal of Vascular Diseases (1999, September), 11

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See detailThe complex injuries produced by percutaneous injection of fluids under very high pressure
Beaujean, M.; Thiry, A.; RADERMECKER, Régis ULg

Poster (2001, June 23)

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See detailComplex invasion pattern of the cerebral cortex bymicroglial cells during development of the mouse embryo.
Avila Macaya, Ariel Salvatore ULg

in Glia (2012)

Microglia are the immune cells of the central nervous system. They are suspected to play important roles in adult synaptogenesis and in the development of the neuronal network. Microglial cells originate ... [more ▼]

Microglia are the immune cells of the central nervous system. They are suspected to play important roles in adult synaptogenesis and in the development of the neuronal network. Microglial cells originate from progenitors in the yolk sac. Although it was suggested that they invade the cortex at early developmental stages in the embryo, their invasion pattern remains largely unknown. To address this issue we analyzed the pattern of cortical invasion by microglial cells in mouse embryos at the onset of neuronal cell migration using in vivo immunohistochemistry and ex vivo time-lapse analysis of microglial cells. Microglial cells begin to invade the cortex at 11.5 days of embryonic age (E11.5). They first accumulate at the pial surface and within the lateral ventricles, after which they spread throughout the cortical wall, avoiding the cortical plate region in later embryonic ages. The invasion of the cortical parenchyma occurs in different phases. First, there is a gradual increase of microglial cells between E10.5 and E14.5. From E14.5 to E15.5 there is a rapid phase with a massive increase in microglia, followed by a slow phase again from E15.5 until E17.5. At early stages, many peripheral microglia are actively proliferating before entering the parenchyma. Remarkably, activated microglia accumulate in the choroid plexus primordium, where they are in the proximity of dying cells. Time-lapse analysis shows that embryonic microglia are highly dynamic cells. [less ▲]

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See detailComplex magnetic ordering in Eu3InP3: A new rare earth metal zintl compound
Jiang, J.; Payne, A. C.; Olmstead, M. M. et al

in Inorganic Chemistry (2005), 44(7), 2189-2197

Eu(3)lnP(3) has been prepared as large single crystals with an indium flux reaction. The structure of the new compound is isotypic to Sr(3)lnP(3) and crystallizes in the orthorhombic space group Pnma with ... [more ▼]

Eu(3)lnP(3) has been prepared as large single crystals with an indium flux reaction. The structure of the new compound is isotypic to Sr(3)lnP(3) and crystallizes in the orthorhombic space group Pnma with unit cell dimensions of a = 12.6517(15) angstrom, b = 4.2683(5) angstrom, and c = 13.5643(14) angstrom (Z = 4, T = 140 K, R1 = 0.0404, wR2 = 0,0971 for all data). The structure consists of one-dimensional chains of corner-shared distorted [InP2P2/2](6-) tetrahedra separated by rows of Eu2+ ions. Two of the three crystallographically distinct europium sites have a short Eu(1)-Eu(2) distance of 3.5954(7) angstrom, which yields Eu-Eu dimers. The Eu-P bond distances range from 2.974(2) to 3.166(2) angstrom. The temperature dependence of the conductivity indicates that Eu(3)lnP(3) is a small band gap semiconductor. Both magnetization and Eu-151 Mossbauer spectral measurements indicate that the europium in Eu(3)lnP(3) is divalent and that at least two magnetic transitions occur. Magnetization studies reveal magnetic transitions at 14, 10.4, and similar to 5 K. These transitions are also observed in heat capacity studies of Eu(3)lnP(3). The Mossbauer spectra indicate that the two europium sites are ordered at 12 K and that all three europium sites are ordered at 8 K. [less ▲]

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See detailComplex Magnetocapillary Microswimmers
Grosjean, Galien ULg; Vandewalle, Nicolas ULg; Lagubeau, Guillaume ULg et al

Poster (2015, July)

When particles are suspended at air-water interfaces in the presence of a vertical magnetic field, dipole-dipole repulsion competes with capillary attraction. Magnetocapillary self-assemblies, composed of ... [more ▼]

When particles are suspended at air-water interfaces in the presence of a vertical magnetic field, dipole-dipole repulsion competes with capillary attraction. Magnetocapillary self-assemblies, composed of N soft-ferromagnetic beads, have been studied as a way to produce controllable micro swimmers at low Reynolds numbers. We hereby investigate the different dynamical behaviors obtained for various self-assemblies. The dynamic interaction of a pair of particles (N = 2) generates a rich behavior at the origin of a non-reciprocal motion, being the major physical ingredient for low Reynolds locomotion. By adding particles to this elementary system up to N = 8, we create new symmetries relevant to generate translational and rotational motions. We propose a model for describing the motion driven by an external field, being the basis for developing elaborated collective behaviors. [less ▲]

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See detailComplex modulation of singing behavior by testosterone in an open-ended learner, the European Starling.
Van Hout, Alain J*-M; Eens, Marcel; Balthazart, Jacques ULg et al

in Hormones & Behavior (2009), 56(5), 564-73

In many temperate zone songbird species males only produce song during the breeding season, when plasma testosterone (T) levels are high. Males of some species sing throughout the year, even when T levels ... [more ▼]

In many temperate zone songbird species males only produce song during the breeding season, when plasma testosterone (T) levels are high. Males of some species sing throughout the year, even when T levels are low, indicating a dissociation between high T levels and song rate. Given that few studies have taken advantage of these species, we compare here song traits expressed under high versus low T concentrations and we study the role of testosterone in adult song learning in the European Starling, an open-ended learner in which repertoire size dramatically increases with age. We performed a detailed comparison of song complexity and song rate between fall and spring in 6-year-old intact male European starlings. In parallel, we investigated whether potential seasonal changes were regulated by the gonadally induced increase in plasma T, by comparing seasonal changes in intact and castrated males of the same age (castrated as juveniles during their first fall) and by subsequently experimentally elevating T in half of the castrated males. While song rate and stereotypy did not differ between intacts and castrates or between fall and spring, both groups increased their average song bout length from fall to spring, but only intact males increased their repertoire size, indicating that effects of seasonal T changes differ between song traits. Intact males overall displayed a larger song repertoire and a longer bout length than the castrates, and implantation with T caused a turnover in repertoire composition in castrates. However, as the castrates had never experienced high T levels and yet displayed a markedly higher repertoire size than that of typical yearling males, this suggests that the progressive increase of song repertoire with age in male starlings is not dependent on gonadal T, although it may be T-enhanced. [less ▲]

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See detailComplex populations of minor pathogens associated with roots from barley plants infected by barley yellow mosaic virus.
Ijdenberg, P.; Kummert, J.; Lepoivre, Philippe ULg

in Parasitica (1986), 42(4),

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See detailThe Complex Problem of Ards: Pathophysiology, Management
Lamy, Maurice ULg

in Acta Anaesthesiologica Belgica (1988), 39(3 Suppl 2), 37-41

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See detailComplex protists in Mesoproterozoic rocks.
Javaux, Emmanuelle ULg; knoll, A. H.; Walter, M.

Conference (2001, April)

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See detailComplex protists in Mesoproterozoic rocks.
Javaux, Emmanuelle ULg; Knoll, A. H.; Walter, Malcolm

Conference (2001)

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See detailComplex regulation of the FRD3 gene in Arabidopsis relatives
Charlier, Jean Benoit; Polese, Catherine ULg; Nouet, Cécile ULg et al

Poster (2014, March 31)

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See detailComplex regulation of the FRD3 gene in Arabidopsis relatives
Charlier, Jean-Benoit ULg; Polese, Catherine ULg; Nouet, Cécile ULg et al

Poster (2013, February 26)

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See detailComplex Risks from Old Urban Waste Landfills: Sustainability Perspective from Iasi, Romania
Ciumasu, I.; Costica, M.; Costica, N. et al

in Journal of Hazardous Materials (2012), 16

Landfills continue to represent the most frequent managerial practice for municipal solid wastes and an increasing and complex problem globally. In certain countries, a transition to an open society and ... [more ▼]

Landfills continue to represent the most frequent managerial practice for municipal solid wastes and an increasing and complex problem globally. In certain countries, a transition to an open society and free market is superimposed on the transition to sustainability, resulting in even higher complexity of management. This paper proposes an approach for problem-structuring of landfills in complex transitions: sustainability or unsustainability of a management approach is determined by a set of sustainability filters that are defined by sets of indicators and prioritized according the systemic concept of sustainability, which says that economy is embedded in society, which is embedded in nature. The writers exercise this approach with an old landfill in Iasi, Romania, and conclude for unsustainability, because the ecological sustainability filter is not successfully passed. Social and economic sustainability filters are also discussed in relation with the ecological sustainability indicators. The described approach allows a coherent, transdisciplinary synthesis of knowledge scattered across various disciplines, a pervasive problem in landfill management. The case study helps distinguish between generally true and context-dependent aspects. [less ▲]

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See detailComplex structure of cometary bands tentatively ascribed to the contour of the solar spectrum
Swings, Polydore ULg

in Lick Observatory Bulletin (1941), 19

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See detailThe complex structure of the Ca II (H and K) lines in the spectrum of the A0ep star with infrared excess HD 190073. II. Interpretation: the selective effect of radiative forces.
Surdej, Jean ULg; Swings, Jean-Pierre ULg

in Astronomy and Astrophysics (1976), 47

Abstract image available at: http://esoads.eso.org/abs/1976A&A....47..121S

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See detailThe complex structure of the Ca II (H and K) lines in the spectrum of the AOep star with infrared excess HD190073
Surdej, Jean ULg; Swings, Jean-Pierre ULg

in Slettebak, A. (Ed.) IAUS 70: Be and Shell Stars (1976)

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