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See detailCoxsackievirus B4 infection of human fetal thymus cells
Brilot, Fabienne; Geenen, Vincent ULg; Hober, Didier et al

in Journal of Virology (2004), 78(18), 9854-9861

The infection of human fetal thymus organ cultures (FTOC) with coxsackievirus B4 E2 (CVB4 E2) was investigated. Both positive- and negative-strand viral RNA were detected by real-time quantitative reverse ... [more ▼]

The infection of human fetal thymus organ cultures (FTOC) with coxsackievirus B4 E2 (CVB4 E2) was investigated. Both positive- and negative-strand viral RNA were detected by real-time quantitative reverse transcription-PCR (RT-PCR) in CVB4 E2-infected FTOC, which supported high yields of virus production (similar to10(6) 50% tissue culture infective doses/ml), and in flow-sorted thymocyte populations for 7 days after inoculation. Cortical CD4(+) CD8(+) thymocytes were found to be the principal targets of infection. Inoculation of human FTOC with CVB4 E2 led to a marked and progressive depletion of immature thymocytes (CD4(+) CD8(+) cells) with no enhancement of Annexin V-positive cells. CVB4 E2 replication caused significant major histocompatibility complex (MHC) class I upregulation on these cells. MHC class I upregulation was correlated with positive- and negative-strand RNA quantitative detection and the release of infectious particles. In addition, chloroquine treatment of FTOC and single-thymocyte suspensions suggested that MHC class I upregulation on thymocytes was the result of direct infection rather than caused by production of soluble factors such as alpha interferon. Thus, CVB4 E2 can infect human fetal thymocytes, which subsequently results in quantitative and qualitative abnormalities of these cells. [less ▲]

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See detailCoxsackievirus B4 Infection of Murine Foetal Thymus Organ Cultures
Brilot, F.; Jaidane, H.; Geenen, Vincent ULg et al

in Journal of Medical Virology (2008), 80(4), 659-66

The infection of foetal thymus with coxsackievirus B4 (CV-B4) E2 has been studied ex vivo by using CD-1 mice on foetal day 14, as a ready source of organs for experimentation to investigate the hypothesis ... [more ▼]

The infection of foetal thymus with coxsackievirus B4 (CV-B4) E2 has been studied ex vivo by using CD-1 mice on foetal day 14, as a ready source of organs for experimentation to investigate the hypothesis of the role of thymic viral infections in the pathogenesis of type 1 diabetes. The replication of CV-B4 E2 in murine foetal thymus organ cultures has been demonstrated by evaluating the levels of positive- and negative-stranded viral RNA in cells by using a real-time quantitative RT-PCR method and by determining titres of infectious viral particles in culture supernatants for 7 days post-infection (p.i.). Staining of tissue sections with an anti-cytokeratin antibody and haematoxylin-eosin showed that CV-B4 infection had no visible effect on cell survival and organ integrity. Cell counts in mock- and virus-infected foetal thymus organ cultures increased from day 1 through day 7, and live cell numbers were comparable in both conditions as shown by Trypan blue exclusion test and 7-amino-actinomycin D staining of thymocytes. Compared with controls on day 7 p.i., cytofluorometric analyses on cells from CV-B4 E2-infected foetal thymus organ cultures displayed a marked increase in the percentage of the most immature CD3(-)CD4(-)CD8(-) thymocytes, and a decrease in the percentage of immature CD3(-)CD4(+)CD8(+) cells, together with an increase in the percentage of mature CD3(+)CD4(+) and CD3(+)CD8(+) cells. These data show that CV-B4 E2 disturbs T-cell maturation and differentiation processes in infected murine foetal thymus organ cultures and provide evidence of a suitable system to investigate the effect of viruses in T-cell differentiation. J. Med. Virol. 80:659-666, 2008. (c) 2008 Wiley-Liss, Inc. [less ▲]

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See detailCo‐construction du langage: le rôle de la mère dans les séquences de rectifications
Collombel, Marie; Maillart, Christelle ULg; Caet, Stéphanie et al

Poster (2010, June 07)

Notre recherche a pour but de mieux repérer ce qui semble faciliter chez l'enfant tout venant son développement linguistique et communicationnel, afin de mettre en place des stratégies éducatives adaptées ... [more ▼]

Notre recherche a pour but de mieux repérer ce qui semble faciliter chez l'enfant tout venant son développement linguistique et communicationnel, afin de mettre en place des stratégies éducatives adaptées aux enfants présentant des troubles du langage. Dans une étude longitudinale des interactions mère-enfant chez trois enfants tout-venant entre 1 an 6 mois et 3 ans, notre équipe s'est penchée sur un phénomène interactionnel au croisement de l'appropriation du système linguistique et de son usage : les rectifications. Nos analyses soulignent le rôle clé de la mère ainsi que les similarités et les différences entre les dyades. La sensibilisation à ces dimensions de la communication auprès de parents d'enfants dysphasiques dans le cadre d'un projet de guidance parentale pourrait favoriser la mise en place de formes d'interaction facilitant le développement langagier et communicationnel des enfants. [less ▲]

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See detailCPB AND AORTIC SURGERY The state of the Art (From a Theoritical to a Practical Approach)
LAGNY, Marc-Gilbert ULg; BLAFFART, Francine ULg; Defraigne, Jean-Olivier ULg

Conference (2012, October 04)

Background Despite recent improvements in prevention, medical treatment [1]and endovascular aortic repair , invasive surgery associated with cardiopulmonary bypass (CPB) remains the best option of ... [more ▼]

Background Despite recent improvements in prevention, medical treatment [1]and endovascular aortic repair , invasive surgery associated with cardiopulmonary bypass (CPB) remains the best option of treatment for some patients. The aim of this study is to make a review of the specific CPB protocols associated with these invasive surgical approaches, based on our clinical experience and according to the recent literature. Up to now, these protocols are not supported by evidence based in medicine (EBM) [2] or in perfusion (EBP). Discrepancies can be observed regarding target temperature , selective cerebral protection , CPB circuit concept and arterial cannulation sites . Variables guidelines are suggested concerning regional flow , transfusion requirements [3], coagulation monitoring blood gas management[4] as for cerebral and CPB monitoring tools. Conclusions In the absence of EBM and EBP in the aortic surgery field, the short and long terms clinical outcomes of the patients elected to invasive surgery for aortic diseases must guide and substantiate our choices of techniques and of CPB protocols. [less ▲]

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See detailCPB AND AORTIC SURGERY: From a theoretical approach to a practical approach
LAGNY, Marc-Gilbert ULg; BLAFFART, Francine ULg

Conference given outside the academic context (2013)

Background Despite recent improvements in prevention, medical treatment [1]and endovascular aortic repair , invasive surgery associated with cardiopulmonary bypass (CPB) remains the best option of ... [more ▼]

Background Despite recent improvements in prevention, medical treatment [1]and endovascular aortic repair , invasive surgery associated with cardiopulmonary bypass (CPB) remains the best option of treatment for some patients. The aim of this study is to make a review of the specific CPB protocols associated with these invasive surgical approaches, based on our clinical experience and according to the recent literature. Up to now, these protocols are not supported by evidence based in medicine (EBM) [2] or in perfusion (EBP). Discrepancies can be observed regarding target temperature , selective cerebral protection , CPB circuit concept and arterial cannulation sites . Variables guidelines are suggested concerning regional flow , transfusion requirements [3], coagulation monitoring blood gas management[4] as for cerebral and CPB monitoring tools. Conclusions In the absence of EBM and EBP in the aortic surgery field, the short and long terms clinical outcomes of the patients elected to invasive surgery for aortic diseases must guide and substantiate our choices of techniques and of CPB protocols. [less ▲]

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See detailCPD - 59 deg 2857 - A new red variable star
Gosset, Eric ULg; Hutsemekers, Damien ULg; Surdej, Jean ULg

in Publications of the Astronomical Society of the Pacific [=PASP] (1985), 97

Two (C3 and C4) of the four comparison stars monitored during the photometric study of the peculiar emission-line star GG Car (Gosset et al., 1984) have been found to be variable. Available photometric ... [more ▼]

Two (C3 and C4) of the four comparison stars monitored during the photometric study of the peculiar emission-line star GG Car (Gosset et al., 1984) have been found to be variable. Available photometric and spectroscopic data allow us to identify C4 with CPD - 59 deg 2857 and classify it as a late-type giant (M3-4 III) belonging to the red variable stars of the semiregular or irregular type. [less ▲]

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See detailCPD-41° 7742: an unusual wind interaction
Sana, H.; Gosset, Eric ULg; Rauw, Grégor ULg et al

in Massive Stars and High-Energy Emission in OB Associations (2005, November 01)

We summarize the results of a multiwavelength observing campaign on the massive eclipsing binary CPD-41° 7742, another remarkable object in the young open cluster NGC 6231. Our campaign relies on high ... [more ▼]

We summarize the results of a multiwavelength observing campaign on the massive eclipsing binary CPD-41° 7742, another remarkable object in the young open cluster NGC 6231. Our campaign relies on high resolution echelle spectroscopy, narrow-band optical photometry, and XMM-Newton X-ray observations. Combined with the spectroscopic analysis, the light curve analysis provides a direct measurement of the masses and sizes of the system components. However, the most outstanding results come from the XMM observations. Our 180 ks campaign towards NGC 6231, and CPD-41° 7742, provides an unprecedented phase coverage of such a close early-type binary. The EPIC-MOS light curves almost fully cover the 2.4 day period of the system and the brightness of the object is sufficient to yield a time resolution as tight as 1 ks. The X-ray flux presents clear variations along the orbit, that we interpret as the signature of an unusual wind interaction. We indeed expect that, in this O+B system, the dominant primary wind crashes into the secondary surface, leading to a wind-photosphere interaction. As a strong support to our interpretation, we provide a geometrical model that associates an extra X-ray emission to the secondary inner surface. Though quite simple, the present model matches the main features of the X-ray light curve. [less ▲]

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See detailCpDNA-based species identification and phylogeography: application to African tropical tree species
Duminil, Jérôme; Heuertz, Myriam; Doucet, Jean-Louis ULg et al

in Molecular Ecology (2010), 19(24), 5469-5483

Despite the importance of the African tropical rainforests as a hotspot of biodiversity, their history and the processes that have structured their biodiversity are understood poorly. With respect to past ... [more ▼]

Despite the importance of the African tropical rainforests as a hotspot of biodiversity, their history and the processes that have structured their biodiversity are understood poorly. With respect to past demographic processes, new insights can be gained through characterizing the distribution of genetic diversity. However, few studies of this type have been conducted in Central Africa, where the identification of species in the field can be difficult. We examine here the distribution of chloroplast DNA (cpDNA) diversity in Lower Guinea in two tree species that are difficult to distinguish, Erythrophleum ivorense and Erythrophleum suaveolens (Fabaceae). By using a blind-sampling approach and comparing molecular and morphological markers, we first identified retrospectively all sampled individuals and determined the limits of the distribution of each species. We then performed a phylogeographic study using the same genetic data set. The two species displayed essentially parapatric distributions that were correlated well with the rainfall gradient, which indicated different ecological requirements. In addition, a phylogeographic structure was found for E. suaveolens and, for both species, substantially higher levels of diversity and allelic endemism were observed in the south (Gabon) than in the north (Cameroon) of the Lower Guinea region. This finding indicated different histories of population demographics for the two species, which might reflect different responses to Quaternary climate changes. We suggest that a recent period of forest perturbation, which might have been caused by humans, favoured the spread of these two species and that their poor recruitment at present results from natural succession in their forest formations. [less ▲]

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See detailThe CphAII protein from Aquifex aeolicus exhibits a metal-dependent phosphodiesterase activity
Kupper, Michaël; Bauvois, Cédric; Frère, Jean-Marie ULg et al

in Extremophiles : Life Under Extreme Conditions (2012), 16(1)

The CphAII protein from the hyperthermophile Aquifex aeolicus shows the five conserved motifs of the metallo-β-lactamase (MBL) superfamily and presents 28% identity with the Aeromonas hydrophila subclass ... [more ▼]

The CphAII protein from the hyperthermophile Aquifex aeolicus shows the five conserved motifs of the metallo-β-lactamase (MBL) superfamily and presents 28% identity with the Aeromonas hydrophila subclass B2 CphA MBL. The gene encoding CphAII was amplified by PCR from the A. aeolicus genomic DNA and overexpressed in Escherichia coli using a pLex-based expression system. The recombinant CphAII protein was purified by a combination of heating (to denature E. coli proteins) and two steps of immobilized metal affinity chromatography. The purified enzyme preparation did not exhibit a β-lactamase activity but showed a metal-dependent phosphodiesterase activity versus bis-p-nitrophenyl phosphate and thymidine 5'-monophosphate p-nitrophenyl ester, with an optimum at 85°C. The circular dichroism spectrum was in agreement with the percentage of secondary structures characteristic of the MBL αββα fold. [less ▲]

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See detailCPLU - rapport d'activité 2010
Willems, Sylvie ULg

Report (2011)

Rapport d'activité de la Clinique Psychologique et Logopédique de l'Université de Liège - Année 2010

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See detailCPLU - rapport d'activité 2011
Willems, Sylvie ULg

Report (2011)

Rapport d'activité de la Clinique Psychologique et Logopédique de l'Université de Liège - 2012

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See detailCPLU - rapport d'activité 2012
Willems, Sylvie ULg

Report (2013)

Rapport reprenant nos prestations, nos équipes, nos cliniciens..

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See detailCPR in pregnancy
Brichant, Jean-François ULg

Conference (2005, September 30)

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See detailCPT-11 and concomitant hyperfractionated accelerated radiotherapy induce efficient local control in rectal cancer patients: results from a phase II
Voelter, V; Zouhair, A; Vuilleumier, H et al

in British Journal of Cancer (2006), 95

Patients with rectal cancer are at high risk of disease recurrence despite neoadjuvant radiochemotherapy with 5-Fluorouracil (5FU), a <br />regimen that is now widely applied. In order to develop a ... [more ▼]

Patients with rectal cancer are at high risk of disease recurrence despite neoadjuvant radiochemotherapy with 5-Fluorouracil (5FU), a <br />regimen that is now widely applied. In order to develop a regimen with increased antitumour activity, we previously established the <br />recommended dose of neoadjuvant CPT-11 (three times weekly 90 mgm 2) concomitant to hyperfractionated accelerated <br />radiotherapy (HART) followed by surgery within 1 week. Thirty-three patients (20 men) with a locally advanced adenocarcinoma of <br />the rectum were enrolled in this prospective phase II trial (1 cT2, 29 cT3, 3 cT4 and 21 cNþ). Median age was 60 years (range 43– <br />75 years). All patients received all three injections of CPT-11 and all but two patients completed radiotherapy as planned. Surgery <br />with total mesorectal excision (TME) was performed within 1 week (range 2–15 days). The preoperative chemoradiotherapy was <br />overall well tolerated, 24% of the patients experienced grade 3 diarrhoea that was easily manageable. At a median follow-up of 2 <br />years no local recurrence occurred, however, nine patients developed distant metastases. The 2-year disease-free survival was 66% <br />(95% confidence interval 0.48–0.83). Neoadjuvant CPT-11 and HART allow for excellent local control; however, distant relapse <br />remains a concern in this patient population. [less ▲]

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See detailCR de Anna Drzewicka, "Volez oïr…". Études sur la littérature française du Moyen Âge, Articles des années 1974-2008
Henrard, Nadine ULg

in Bulletin Bibliographique de la Société Rencesvals (2014), 2013-2014(45), 208-212

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See detailCR de Cl. FAURIEL, Histoire de la poésie provençale
Henrard, Nadine ULg

in Le Moyen Âge (2013), CXIX(3-4), 762-764

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See detailcr de E. Wente, Letters from Ancient Egypt
Winand, Jean ULg

in Chronique d'Egypte : Bulletin Périodique de la Fondation Egyptologique Reine Elisabeth (1996), 71(141), 57-62

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See detailcr de J. Cerny, Papyrus hiératiques de Deir el-Médineh, t. II
Winand, Jean ULg

in Chronique d'Egypte : Bulletin Périodique de la Fondation Egyptologique Reine Elisabeth (1993), 68

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See detailCRA-W's Committee of Intervention : analyse of catchments polluted with pesticide
Noel, Stephanie; Bah, Boubacar Billo ULg; Colinet, Gilles ULg et al

in 10th International Congress on Mechanization and Energy in Agriculture, 14-17 October 2008, Antalya-Türkiye - Prodeedings (2008)

In the Walloon Region of Belgium, a commlttee of Intervention has been created to Investigate problems of pesticide contamination of various catchments use for drinking water production. This commlttee ... [more ▼]

In the Walloon Region of Belgium, a commlttee of Intervention has been created to Investigate problems of pesticide contamination of various catchments use for drinking water production. This commlttee Involves the Agricultural Research centre - Wallonla (CRA-W, project coordinator) and sorne University experts. It ls funded by the Société Publique de Gestion des Eaux (SPGE). The diagnosis method, base on the AQUAPLAlNE method (Arvalis - France), conslsts of 4 steps. The first step Is the preparation of diagnosls (at the office) that takes into account the paper r1sk of active ingredients. and thelr uses, the identification of the agrtcultural parcels, the collection of cartographie and numeric data, the description of the hydrogeological and pedological contexts and the study of the meteorologlcal data ln relation with the period of pollution. The second step conslsts of maklng a plot diagnosls (on the field) ta Identify the way of transfer inside the plot and collecting data. At the thlrd step, the people who can apply ppp treatment close to the catchment are met (farmers and city services). Information are coJ!ected on treatments applied and on the state of parcels. Based on the hypathesls of pollution cause, the committee proposes solution to solve the problem. One of the catchment that has been investigated by the commlttee is located at Biesmerée, (Namur province, ln Belglum). A temporally contamination was caused by 4 pesticides: chlortoluron, isoproturon, trifluralln and diflufenlcan. After Investigations, it seems that the pollution was probably due ta the hydrogeologlcal context . As the river Is locally perched over the aqulfer, the presence of Poly-aromatic hydrocarbons (PAHs) could be due ta the Infiltration of surface water inside the catchment or/and to the presence of a sinkhole temparaHy activated durlng river flood period. Infiltration rate has ta be assessed and river bank Impermeabilization Is recommended [less ▲]

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