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See detailCulture et gestion en Belgique : un modèle spécifique de concertation sociale et de relations avec les syndicats
Cornet, Annie ULg; Vandenbergh, J. M.; Xhenseval, J. M.

in Davel, E.; Dupuis, J. P.; Chanlat, J. F. (Eds.) Gestion en contexte interculturel : Approches, problématiques, pratiques et plongées (2008)

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See detailCulture in vitro de Jatropha curcas L.
Medza Mve, Samson Daudet ULg; Mergeai, Guy ULg; Baudoin, Jean-Pierre ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2011), 15(4), 567-574

The extension of Jatropha curcas L. cultivation as a biofuel feedstock species requires the distribution of a very large number of plants to the producers in a very short period of time. These plants have ... [more ▼]

The extension of Jatropha curcas L. cultivation as a biofuel feedstock species requires the distribution of a very large number of plants to the producers in a very short period of time. These plants have to be able to give a high oil yield and be morphologically and phenotypically homogeneous to facilitate cultural operations. If high oil content can be obtained by varietal selection, the achievement of homogeneous material passes by the in vitro propagation. Various methods of mass production of plant material by axenic culture have been published. This study reviews the protocols published for in vitro propagation of J. curcas and discusses their applicability to an industrial scale. [less ▲]

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See detailLa culture mathématique à 15 ans. Premiers résultats de PISA 2012 en Fédération Wallonie-Bruxelles
Demonty, Isabelle ULg; Blondin, Christiane ULg; Matoul, Anne ULg et al

in Les Cahiers des sciences de l'éducation (2013), 34

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See detailLa "culture médiatique" au XIXe siècle
Durand, Pascal ULg

in Les Dossiers de l'ORM (1999), 6

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See detailLa “culture médiatique” au XIXe siècle. Essai de définition-périodisation
Durand, Pascal ULg

in Quaderni : la Revue de la Communication (1999)

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See detailCulture of Gingival Fibroblasts on Bioabsorbable Regenerative Materials in Vitro
Simain-Sato, Franklin ULg; Lahmouzi, Jamila ULg; Kalykakis, G. K. et al

in Journal of Periodontology (1999), 70(10), 1234-9

BACKGROUND: The use of membranes in guided tissue regeneration (GTR) can limit the apical migration of gingival cells and favor the establishment of new attachment by periodontal ligament fibroblasts ... [more ▼]

BACKGROUND: The use of membranes in guided tissue regeneration (GTR) can limit the apical migration of gingival cells and favor the establishment of new attachment by periodontal ligament fibroblasts. However, gingival recession during healing following GTR has been described as a frequent complication. The purpose of this study was to determine if gingival fibroblasts are affected by the composition of the bioabsorbable membranes used in mucogingival surgery. METHODS: Two type of bioabsorbable regenerative materials were used as cell carriers. Wistar rat gingival fibroblasts (RGF) were obtained from attached gingiva, cut into small fragments, and placed in culture dishes. When confluent, cells were detached using trypsin and identified as "first transferred cells" (P1). At the third passage (P3), cell count, trypan blue exclusion test, acid phosphatase activity, DNA synthesis, phase contrast microscopy, and scanning electron microscopy were performed. The cells were then placed in wells containing the membranes and incubated for 72 hours. RESULTS: When examined under microscopy, the control wells (without membranes) showed one cell type with the elongated appearance characteristic of fibroblasts. The wells with membranes showed an altered cell morphology with a high proportion of cell fragments regardless of the type of membrane used. CONCLUSIONS: These results suggest that cell carrier membranes could affect RGF morphology and thus alter gingival tissue healing following GTR. [less ▲]

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See detailCulture of lactic acid bacteria : chemical engineering aspects
Delvigne, Frank ULg; Thonart, Philippe ULg

Conference (2003, October 10)

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See detailCulture of palatal shelves treated with neuramimidase
Baeckeland, E.; Renard, A. M.; Heinen, Ernst ULg et al

in Verhandlungen der Anatomischen Gesellschaft (1982), 76

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See detailCulture of very young Phaseolus vulgaris L. pods and plantlet regeneration.
Geerts, P.; Toussaint, André ULg; Mergeai, Guy ULg et al

in Acta Horticulture 560 : Proceedings of the Fourth International Symposium on In Vitro Cultureand Horticultural Breeding (2001)

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See detailCulture populaire, culture de masse ou culture de mass-médias ? Autour de cinq thèses moins une d’Antonio Gramsci
Durand, Pascal ULg

in Quaderni : la Revue de la Communication (2005), 57(printemps), 73-83

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See detailLa culture scientifique du début du XIIe à la fin du XVe siècle.
Halleux, Robert ULg; Vandersmissen, Jan ULg

in Halleux, Robert; Opsomer, Carmélia; Vandersmissen, Jan (Eds.) Histoire des sciences en Belgique de l’Antiquité à 1815. (1998)

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See detailCulture, identité, politique
Martiniello, Marco ULg

Scientific conference (2011, May 09)

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See detail Culture, identité, politique
Martiniello, Marco ULg

Scientific conference (2011, May 09)

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See detailCulture, vous avez dit Kultur? Kulturarbeit zwischen Selbstbestimmung und Selbstfindung
Brüll, Christoph ULg

in Lejeune, Carlo; Brüll, Christoph (Eds.) Grenzerfahrungen. Eine Geschichte der Deutschsprachigen Gemeinschaft Belgiens. Vol. 5: Säuberung, Wiederaufbau, Autonomiediskussionen (1945-1973) (2014)

Kulturarbeit war seit den 1920er Jahren in den ehemaligen Kreisen Eupen-Malmedy politisiert und seit den 1930er Jahren ideologisiert. Nach 1945 gab es keine Kulturarbeit in der Muttersprache dieser ... [more ▼]

Kulturarbeit war seit den 1920er Jahren in den ehemaligen Kreisen Eupen-Malmedy politisiert und seit den 1930er Jahren ideologisiert. Nach 1945 gab es keine Kulturarbeit in der Muttersprache dieser Menschen mehr. Die Region sollte französisiert werden. Erst Mitte der 1950er Jahre begannen Bürger in den deutschsprachigen Gemeinden, sporadisch aktiv Kulturarbeit zu betreiben. Diese stand unmittelbar in einem politischen Spannungsfeld, das immer wieder neu zwischen Akteuren an der Basis, dem Bezirkskommissar Hoen, der Provinz Lüttich und den kulturellen Dienststellen des Zentralstaates Belgiens ausgelotet werden musste. Christoph Brüll stellt sich mehrere Fragen: Haben sich hier Grundpositionen herausgebildet, die auch den politischen Diskurs später bestimmten? Welcher Akteur verfolgte welche Ziele mit welchen Mitteln? War der kulturelle Frühling Initialzündung, Begleiter oder nur Folge des „politischen Frühlings“ in der Region? Er liefert zahlreiche neue Fakten und formuliert neue Meinungen zu einem Politikbereich, der die ureigenste Besonderheit der deutschsprachigen Belgier widerspiegelt. Der Titel „Kulturarbeit zwischen Fremdbestimmung und Selbstfindung“ verdeutlicht auch hier, dass er transregionale Einflüsse aufgreift, um diese regional runterzubrechen. [less ▲]

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See detailCultured Astroglia Release a Neuronotoxic Activity That Is Not Related to the Excitotoxins
Leprince, Pierre ULg; Lefebvre, Philippe ULg; rigo, Jean-Michel et al

in Brain Research (1989), 502(1), 21-7

Neuronal death after brain injury is thought to be in part the result of the activity of the excitotoxins, a family of excitatory amino acids which are released by neurones. We have also described an ... [more ▼]

Neuronal death after brain injury is thought to be in part the result of the activity of the excitotoxins, a family of excitatory amino acids which are released by neurones. We have also described an astroglial cell-derived neuronotoxic activity of low molecular weight whose release can be induced by depolarizing events such as an increase in extracellular potassium concentration. We study here the relationship between this astroglia-derived neuronotoxic activity present in astroglia-conditioned medium (ACM) and the excitotoxins. Using a colorimetric assay of neuronal survival, we show that the ACM neuronotoxic activity, is able to induce the death of all types of neurones tested, including those which are insensitive to excitotoxins. Furthermore, the ACM neuronotoxic activity does not require for its action the extracellular ionic composition which is needed for the activity of excitotoxins. Finally, the ACM neuronotoxic activity is not blocked by competitive or non-competitive antagonists of the various classes of excitotoxin receptors. Those data demonstrate that the astroglia-derived neuronotoxic activity is not related to the excitotoxins. Still, because astrocytes can also be depolarized by members of the excitotoxin family, the possibility exists that the release of astroglia-derived neuronotoxic activity would follow the rise in extracellular excitatory amino acid concentration during nervous system injury. [less ▲]

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See detailCultured human bronchial epithelial cells: blood group antigens, keratin, collagens, and fibronectin
Stoner, G. D.; Katoh, Y.; Foidart, Jean-Michel ULg et al

in In Vitro (1981), 17(7), 577-87

Immunofluorescence and immunoperoxidase methods were used to identify constituents and products of cultured human bronchial epithelial cells and fibroblasts. Epithelial cells, but not fibroblasts, from ... [more ▼]

Immunofluorescence and immunoperoxidase methods were used to identify constituents and products of cultured human bronchial epithelial cells and fibroblasts. Epithelial cells, but not fibroblasts, from patients with blood Types A or B reacted to the respective antisera to either the A or B blood group antigens. However, neither the epithelial cells nor the fibroblasts from patients with blood type O[H] reacted with the anti-H antisera. Epithelial cells in primary culture reacted with antibody to prekeratin proteins from human stratum corneum and fibroblasts did not react. Moreover, keratin filaments were assembled in vitro from proteins isolated from the epithelial cells. These immunological and biochemical data support previous morphological observations that human bronchial epithelial cells in primary culture shift progressively from a mucociliary epithelium to a keratinizing epithelium. Epithelial cells and fibroblasts could also be identified by their reactivity to anti-collagen antibodies. Fibroblasts reacted strongly with antibodies to Types I and III collagens and epithelial cells did not. On the other hand, epithelial cells reacted weakly with antibodies to Type IV collagen and fibroblasts were completely negative. Both epithelial cells and fibroblasts reacted with antibody to fibronectin; however, the distribution of fibronectin differed in the two cell types. In epithelial cells, fibronectin was restricted to the cell surface, whereas in fibroblasts it was found on the cell surface and in the extracellular matrix where fibrils of fibronectin were both cell associated and deposited on the surface of the dish where the fibroblasts had migrated. [less ▲]

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See detailCultured neurons release an inhibitor of astroglia proliferation (astrostatine).
Rogister, Bernard ULg; Leprince, Pierre ULg; Bonhomme, Vincent ULg et al

in Journal of Neuroscience Research (1990), 25(1), 58-70

Using in vitro techniques, we looked for a possible downregulation of rat astroglia proliferation by neuronal cells. We demonstrate that medium conditioned by 7-day-old rat cerebellar granule neurons or ... [more ▼]

Using in vitro techniques, we looked for a possible downregulation of rat astroglia proliferation by neuronal cells. We demonstrate that medium conditioned by 7-day-old rat cerebellar granule neurons or by 16-day-old rat embryo hippocampal neurons strongly inhibits the proliferation of cultured astroglial cells. Two neuronal cell lines, the PC12 rat pheocromocytoma and the neuro 2A (N2A) murine neuroblastoma also release such an activity. This release in N2A-conditioned medium (CM) occurs when the cells are at high density and show a low proliferation rate. This activity is present in media conditioned by neuronal cells, but not in media conditioned by normal astrocytes, by two glioma cell lines, or by one fibroblastic cell line. This proliferation inhibitor addresses normal astrocytes: the proliferation of two glioma cell lines, of a fibroblastic cell line, and of the two neuronal cell lines (PC12, N2A) is not inhibited by N2A CM. Moreover, this activity is directed against type 1 astrocytes, but not against type 2. Using three different assays, we demonstrate that DNA synthesis by astroglial cells is inhibited. N2A CM has no cytotoxic effect on astrocytes and does not modify their overall protein synthesis. Using affinity and gel filtration chromatography, we show that this activity is associated with a protein whose molecular weight ranges between 15 and 20 kDa. The possible relationship between this N2A cell-derived astroglia proliferation inhibitor and other types of potential glial proliferation inhibitors has been investigated. A brain glycoprotein immunologically related to epidermal growth factor receptor (EGFR) was reported to inhibit astroglial cell proliferation in vitro. Using polyclonal and monoclonal antibodies against EGFR, we were unable to immunoprecipitate the astrocyte proliferation inhibitor in N2A CM or to demonstrate by immunoblotting the presence of an EGFR-like immunoreactivity in the N2A CM or in the active chromatographic fractions of N2A CM. Transforming growth factor beta (TGF beta) is a well-known modulator of the proliferation of various cell types and was shown to be present in N2A CM. Using a polyclonal anti-TGF beta antibody that recognizes TGF beta on Western blots of N2A CM, we were unable to immunoprecipitate the astrocyte proliferation inhibitor of N2A CM. It seems thus far that the neuronal astroglia proliferation inhibitor is a new protein for which we propose the name astrostatine. [less ▲]

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See detailCultured Oligodendrocyte Progenitors Derived from Cerebral Cortex Express a Glycine Receptor Which Is Pharmacologically Distinct from the Neuronal Isoform
Belachew, Shibeshih ULg; Rogister, Bernard ULg; Rigo, Jean-Michel et al

in European Journal of Neuroscience (1998), 10(11), 3556-64

Using the whole-cell patch-clamp technique, we demonstrate glycine-induced currents in oligosphere-derived oligodendrocyte progenitors cultured from newborn rats. Similar inward currents are also ... [more ▼]

Using the whole-cell patch-clamp technique, we demonstrate glycine-induced currents in oligosphere-derived oligodendrocyte progenitors cultured from newborn rats. Similar inward currents are also triggered by beta-alanine and taurine, two established glycine receptor agonists. In our recording conditions, glycine-gated currents in oligodendrocyte progenitors reverse about 0 mV and are reversibly inhibited by the glycine competitive antagonist strychnine, the Cl- channel blocker picrotoxinin and the non-competitive antagonist cyanotriphenylborate. The oligodendrocyte progenitors glycine receptor (GlyR) differs from the corresponding neuronal receptor: [3H]strychnine binding data and the strychnine inhibition curve of glycine-induced currents in oligodendrocyte progenitor cultures suggest the existence of two strychnine binding sites on the oligodendroglial GlyR. Using total RNA isolated from oligodendrocyte progenitors cultures, reverse transcription-polymerase chain reaction analysis of glycine receptor subunit expression shows the presence of alpha2 and beta subunits and immunocytochemical stainings confirm that this GlyR contains an alpha subunit which is not alpha1. The molecular structure of the oligodendroglial GlyR could be either homopentameric alpha2 or heteromeric alpha2beta but in both cases, the sequence of the alpha2 or beta subunits have to be different from the known neuronal sequences in order to explain, respectively, the cyanotriphenylborate (alpha2) and picrotoxinin (beta) sensitivities. This work thus demonstrates that GlyR are expressed by oligodendrocytes obtained not only from spinal cord but also from supraspinal structures. The pharmacological properties and presumably the molecular structure of oligodendroglial GlyR are original. The physiological meaning of the presence of such receptors on developing and mature oligodendrocytes remains unknown. [less ▲]

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