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See detailConstruction navale I, calcul de la structure
Marchal, Jean ULg

Learning material (1987)

Detailed reference viewed: 71 (1 ULg)
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See detailConstruction of a bovine genomic library of large yeast artificial chromosome clones
Libert, F.; Lefort, A.; Okimoto, R. et al

in Genomics (1993), 18(2), 270-276

We have constructed a yeast artificial chromosome (YAC) library derived from bull fibroblasts in primary culture. The library consists of 21,500 clones arranged on 224 gridded plates (96 or 8×12 positions ... [more ▼]

We have constructed a yeast artificial chromosome (YAC) library derived from bull fibroblasts in primary culture. The library consists of 21,500 clones arranged on 224 gridded plates (96 or 8×12 positions) and in 2×224 96-well microplates maintained as permanent frozen stocks. An average insert size of 750 kb was estimated from the analysis of 200 randomly selected YACs, giving a sixfold coverage of the bovine genome for the total library. A screening strategy based on the polymerase chain reaction that allows the identification of individual clones has been devised. Screening of the library with six locus-specific markers of interest led to the isolation of 34 positive clones [less ▲]

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See detailConstruction of a medium-density horse gene map
Perrocheau, M; Chadi, S; Mata, X et al

in Animal Genetics (2006)

A medium-density map of the horse genome (Equus caballus) was constructed using genes evenly distributed over the human genome. Three hundred and twenty-three exonic primer pairs were used to screen the ... [more ▼]

A medium-density map of the horse genome (Equus caballus) was constructed using genes evenly distributed over the human genome. Three hundred and twenty-three exonic primer pairs were used to screen the INRA and the CHORI-241 equine BAC libraries by polymerase chain reaction and by filter hybridization respectively. Two hundred and thirty-seven BACs containing equine gene orthologues, confirmed by sequencing, were isolated. The BACs were localized to horse chromosomes by fluorescent in situ hybridization (FISH). Overall, 165 genes were assigned to the equine genomic map by radiation hybrid (RH) (using an equine RH5000 panel) and/or by FISH mapping. A comparison of localizations of 713 genes mapped on the horse genome and on the human genome revealed 59 homologous seg- ments and 131 conserved segments. Two of these homologies (ECA27/HSA8 and ECA12p/ HSA11p) had not been previously identified. An enhanced resolution of conserved and rearranged chromosomal segments presented in this study provides clarification of chromosome evolution history. [less ▲]

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See detailConstruction of a set of vectors allowing inducible production of siRNA in Schizosaccharomyces pombe.
Stroobants, Aurore ULg; Tafforeau, Lionel; Guiguen, Allan et al

Poster (2004, May 14)

RNA interference (RNAi) is a sequence-specific gene silencing mechanism. It is induced by the formation of dsRNA that are recognised by the Dicer complex and processed into 21-23 long oligonucleotides ... [more ▼]

RNA interference (RNAi) is a sequence-specific gene silencing mechanism. It is induced by the formation of dsRNA that are recognised by the Dicer complex and processed into 21-23 long oligonucleotides called siRNA (short interfering RNA). Subsequently, RISC (RNA-Inducing Silencing Complex) binds siRNA that targets the complex towards its homologous mRNA (DYKXHOORN et al., 2003) which is eventually degraded. In contrast to budding yeast, the entire pathway is conserved in the fission yeast Schizosaccharomyces pombe, making it a valuable organism to both study physiological RNAi and to use it as a inducible gene knock-down tool. In an attempt to apply this method in the fission yeast, we are using three different approaches to produce siRNA. In each case, a vector containing a regulatable promoter activated in presence of tetracycline (tTA') (GOSSEN et al., 1995) is generated and the ura4 marker required for growth on medium lacking uracil serves as reporter. First, a vector expressing the full lenght antisens RNA of ura4 (800 nucleotides) (RAPONI and ARNDT, 2003) is used. Second, we are trying to generate much shorter dsRNA where both strands are linked by either a short hairpin of 25 nucleotides (BRUMMELKAMP et al., 2002) or a longer one of 350 nucleotides (SCHRAMKE and ALLSHIRE, 2003). The ability of these different dsRNA to induce silencing of ura4 will be presented. [less ▲]

Detailed reference viewed: 21 (4 ULg)
See detailConstruction of an agrometeorological model for forecasting agricultural production in the twelve countries of the european economic community
De Bast, Albert; Palm, Rodolphe ULg

in Toselli, F.; Meyer-Roux, J. (Eds.) Conference on the Application of Remote Sensing to Agricultural Statistics - Proceedings (1990)

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See detailConstruction of an interactomic map of Ets factors and identification of new functions in mRNA processing
Rambout, Xavier ULg

Doctoral thesis (2013)

The family of Ets factors is one of the largest families of human transcription factors. This thesis aimed at molecularly and biologically defining these well known though not well-understood ... [more ▼]

The family of Ets factors is one of the largest families of human transcription factors. This thesis aimed at molecularly and biologically defining these well known though not well-understood transcription factors using a system biology approach. Combination of interactomic and bioinformatics tools gave rise to the first interactome of the human Ets factors built on more than 400 interactions with nearly 300 interaction partners. We fragmented our interactome in 24 functional highly intraconnected sub-networks (clusters, or CL) and highlighted a new role for Ets transcription factors in mRNA processing (CL1). Steady-state levels of mRNAs result from the balance between transcription and mRNA decay, two events sitting at both ends of the mRNA life. In the traditional and still prominent view, these events are spatially, functionally and temporally independent. Here, we showed that the Erg subfamily of Ets factors (composed of the three members ERG, FLI1, and FEV) regulate mRNA decay of specific targets via promoter-mediated mRNA imprinting with sequence-specific RNA-binding proteins (CL1) and the CCR4-NOT deadenylation complex. This constitutes the first evidence, regardless of the organism, that DNA-binding transcription factors recruit RNA-binding proteins and mRNA decay components to control the degradation of specific mRNAs. This is also the most detailed demonstration of a functional coupling between transcription and mRNA decay machineries in humans. We showed that ERG promoted mRNA decay of key mitotic regulators, among which the Aurora kinases Aurora A and B. Depletion of ERG prevented degradation of Aurora A and B mRNAs during mitosis, leading to aberrant levels of Aurora proteins, accumulation of centrosome and mitotic spindle defects, and ultimately mitosis blockage. This consists in a significant advance in understanding of mitosis progression that was until now thought to be exclusively regulated by post-translational modifications and proteasomal degradation of proteins. Our results show that, in the contrary of bulk mRNAs whose decay is inhibited during mitosis, degradation of specific transcripts is a prerequisite for normal mitosis progression. [less ▲]

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See detailConstruction of individual breeding values for feed intake of Piétrain boars based on mean pen feed intake, weight and weight gain test station records
Dufrasne, Marie ULg; Jaspart, Véronique; Wavreille, José et al

in Journal of Animal Science (2011), 89(E-Suppl.1), 474-475

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See detailConstruction of regular languages and recognizability of polynomials
Rigo, Michel ULg

in Discrete Mathematics (2002), 254(1-3), 485-496

A generalization of numeration systems in which NI is recognizable by finite automata can be obtained by describing a lexicographically ordered infinite regular language. We show that if P is an element ... [more ▼]

A generalization of numeration systems in which NI is recognizable by finite automata can be obtained by describing a lexicographically ordered infinite regular language. We show that if P is an element of Q[x] is a polynomial such that P(N) subset of N then there exists a numeration system in which the set of representations of P(N) is regular. The main issue is to construct a regular language with a complexity function equals to P(n + 1) - P(n) for n large enough. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailConstruction of transformed yeast strains for agro-food applications and development of systems for stabilizing the genetic material introduced by genetic engineering into the food yeast Saccharomyces cerevisiae.
Broman, K.; Jauniaux, J. C.; Vandenbol, Micheline ULg et al

in Biomolecular Engineering in the European Community. Achievements of the Research Programme (1982-1986) (1986)

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See detailThe construction of workers’ rights consciousness through legal intermediations: the case of employment discrimination in Belgium
Lejeune, Aude ULg; Orianne, Jean-François ULg

in International Journal of Discrimination and the Law (in press)

Through their different encounters with union, court, and government equality agency lawyers, workers report diverse understandings of their personal experience of injustice in the workplace. This paper ... [more ▼]

Through their different encounters with union, court, and government equality agency lawyers, workers report diverse understandings of their personal experience of injustice in the workplace. This paper examines workers’ experiences of discrimination and the role legal professionals play in litigating these issues in Belgium. Bringing together legal and rights consciousness studies and the sociology of intermediation and tracking different stages in the construction of discrimination cases, from the moment when a future litigant describes an event as an injustice to the moment when the judge recognises a discriminatory behaviour (or conversely, dismisses a case), we suggest several possible empirical explanations of the way in which interactions with legal intermediaries affect workers’ rights consciousness. Because we refer to socio-legal studies from common law countries, this paper also calls into question how best to import these studies to assist in analysing legal mobilisations and legal consciousness in continental Europe. [less ▲]

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See detail“Construction paradoxale d’une altérité intégrée : autoportrait de Barack Obama.” In Intégration de l’altérité, formes et procédure : regards croisés sur les aires anglophones et hispanophones.
Bada, Valérie ULg

in Chini, Danielle; Laverrou, Florence; Parsons, Michael (Eds.) Intégration de l’altérité, formes et procédure : regards croisés sur les aires anglophones et hispanophones. (2011)

Detailed reference viewed: 14 (1 ULg)
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See detailLa construction professionnelle des troubles de l’employabilité
Orianne, Jean-François ULg

Conference (2004, September 24)

Detailed reference viewed: 24 (4 ULg)
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See detailLa construction sociale de l'économie sociale en Corée du Sud
Eum, Hyung Sik ULg; Bidet, Eric

Scientific conference (2013, October 22)

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See detailConstruction sociotechnique de la qualité
Stassart, Pierre M ULg; Collet, Eric

in Mormont, Marc; Van Huylenbroeck, Guido (Eds.) A la recherche de la qualité (2001)

Detailed reference viewed: 28 (2 ULg)