Deliverable D1.5 & D1.11 “Annual reporting PAR-PMR ” Projet STREP FP6 (2007-2009)

Report (2008)

Deliverable D2.1 “Shipyard requirements” - IMPROVE”, Projet STREP FP6 (2007-2009)

Report (2007)

Deliverable D2.5 “Report on definition of multi-stakeholder design problems for LNG, RO-PAX and tanker” - IMPROVE”, Projet STREP FP6 (2007-2009)

Report (2007)

Deliverable D2.6 “Catalogue of the Selected Methods” - IMPROVE”, Projet STREP FP6 (2007-2009)

Report (2007)

Deliverable D3.3 “Rational models to assess fatigue at the early design stage”, Projet STREP FP6 (2007-2009)

Report (2008)

Deliverable D4.2 “Data preparation and development of the selected modules to assess the fabrication costs and simulate the production” Projet STREP FP6 (2007-2009)

Report (2008)

Deliverable D5.2 “Specification, implementation and validation of file exchange interface ” Projet STREP FP6 (2007-2009)

Report (2008)

Deliverable D6.2 “LNG Structural optimization ” Projet STREP FP6 (2007-2009)

Report (2009)

Delivering bad news to customers: The role of employee emotional and technical competences

Conference (2011, July)

Delivery of granulocyte-macrophage colony-stimulating factor in bioadhesive hydrogel stimulates migration of dendritic cells in models human papillomavirus-associated (pre)neoplastic epithelial lesions

in Antimicrobial Agents and Chemotherapy (2004), 48(11), 4342-4348

Because of the central role of dendritic cells and/or Langerhans cells(DC/LC) in the induction of cellular immune responses, pharmacological agents that modulate the recruitment of these cells might have ... [more ▼]

Because of the central role of dendritic cells and/or Langerhans cells(DC/LC) in the induction of cellular immune responses, pharmacological agents that modulate the recruitment of these cells might have a clinical interest. The present study was designed to evaluate the capacity of several pharmaceutical formulations to topically deliver granulocyte-macrophage colony-stimulating factor (GM-CSF) on human papillomavirus (HPV)-associated genital (pre)neoplastic lesions. The formulations were evaluated for their bioactivity and for their potential to recruit DC in organotypic cultures of HPV-transformed keratinocytes. We found that a bioadhesive polycarbophil gel (Noveon) at pH 5.5 is able to maintain the bioactivity of GM-CSF at 4 or 37°C for at least 7 days, whereas a decreased activity of GM-CSF was observed when the molecule is included in other polymer gels. GM-CSF incorporated in the polycarbophil gel was also a potent factor in enhancing the colonization of DC into organotypic cultures of HPV-transformed keratinocytes since the infiltration of DC in the in vitro-formed (pre)neoplastic epithelium was very low under basal conditions and dramatically increased in the presence of GM-CSF gel. We next demonstrated that GM-CSF incorporated in polycarbophil gel induces the recruitment of human DC in a human (pre)neoplastic epithelium grafted into NOD/SCID mice. The efficacy of GM-CSF in this formulation was equivalent to that observed with liquid GM-CSF. These results suggest that GM-CSF incorporated in polycarbophil gel could play an important role in the recruitment of DC/LC in mucosal surfaces and be useful as a new immunotherapeutic approach for genital HPV-associated (pre)neoplastic lesions. [less ▲]