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See detailClonage et sequencage de genes.
Burny, A.; Chaput, M.; Claes, V. et al

Book (1988)

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See detailClonage humain et processus de prise de décision publique
Brunet, Sébastien ULg

Conference given outside the academic context (2001)

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See detailClonage par greffe de blastomère: naissance d'un premier veau
Ectors, Fabien ULg; Delval, Alain; Touati, Kamal ULg et al

in Annales de Médecine Vétérinaire (1993), 137(8), 573-574

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See detailLe clonage par transfert de noyau dans l'espèce bovine
Ectors, Fabien ULg; Delval, Alain; Beckers, Jean-François ULg et al

in Annales de Médecine Vétérinaire (1997), 141

A all in vitro cloning technique was developed in wich embryos from the first cycle nuclear transfer (cloning) were used as blastomere donor for the second cycle nuclear transfer (re-cloning). Such method ... [more ▼]

A all in vitro cloning technique was developed in wich embryos from the first cycle nuclear transfer (cloning) were used as blastomere donor for the second cycle nuclear transfer (re-cloning). Such method permitted to produce 14,5% of morulae and 14,9% of blastocysts after the first and second cycle of nuclear transfer, respectively. The rates of birth obtained after transfer of such embryos were 21,4% and 20,5% for first and second cyle respectively, corresponding to 6 and 5 calves for 28 and 24 transferred embryos. Unfortunately, gestation patholgies and an increase of birth weights were observed. It seems that the in vitro presence of gametes and/or embryos may be responsible of an alteration in the control of gene expression. [less ▲]

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See detailClonage par transfert de noyau dans l'espèce bovine: premiers résultats
Ectors, Fabien ULg; Delval, Alain; Touati, Kamal ULg et al

in Annales de Médecine Vétérinaire (1993), 137(6), 427-431

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See detailLe clonage par transfert de noyau dans l'espèce bovine: premiers résultats
Ectors, Francis ULg; Ectors, Fabien ULg; Delval, Alain et al

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1993), 148

In 1987, Prather et al. have performed the first embryo cloning by nuclear transfer in the bovine species. Since, many researchers try to develop and to apply the technique. While the enucleation of the ... [more ▼]

In 1987, Prather et al. have performed the first embryo cloning by nuclear transfer in the bovine species. Since, many researchers try to develop and to apply the technique. While the enucleation of the recipient oocyte, the injection of the donor blastomere and the fusion procedure are now well controlled, on the other hand, maturation and activation as the development and freezing of the cloned embryos need tobe more investigated. The cloned embryo is more fragile. An increase in embryonic mortality is observed after transfer in a recipient cow. [less ▲]

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See detailLe clonage par transfert de noyau dans l'espece bovine: resultats et perspectives.
Ectors, Fabien ULg

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1996), 151(12), 493-9

A all in vitro cloning technique was developed in which the reconstituted embryos from the first cycle nuclear transfer (cloning) were used as blastomere donor for the second cycle nuclear transfer ... [more ▼]

A all in vitro cloning technique was developed in which the reconstituted embryos from the first cycle nuclear transfer (cloning) were used as blastomere donor for the second cycle nuclear transfer (recloning). Such method permitted to produce 14.5% of morulae and 14.9% of blastocysts after the first and second cycles of nuclear transfer, respectively. The rates of birth obtained after transfer of such embryos were 21.4% et 20.8% for first and second cycles respectively, corresponding to 6 et 5 calves for 28 et 24 transferred embryos. Unfortunately, gestation pathologies and an increase of birth weights were observed. It seems that the in vitro presence of gametes and/or embryos may be responsible of an alteration in the control of gene expression. [less ▲]

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See detailLe clonage par transfert de noyaux dans l'espèce bovine
Ectors, Fabien ULg

Doctoral thesis (1995)

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See detailClonage, caractérisation et étude de la régulation transcriptionnelle du gène Aox1 encodant l'oxydase alternative chez Chlamydomonas reinhardtii
Baurain, Denis ULg

Doctoral thesis (2003)

Au sein de la membrane interne des mitochondries, quatre complexes multiprotéiques sont impliqués dans le transfert des électrons depuis les équivalents réducteurs jusqu'à l'oxygène moléculaire. L'énergie ... [more ▼]

Au sein de la membrane interne des mitochondries, quatre complexes multiprotéiques sont impliqués dans le transfert des électrons depuis les équivalents réducteurs jusqu'à l'oxygène moléculaire. L'énergie associée à ce transport au travers des complexes I, III et IV est couplée à la synthèse d'ATP par l'intermédiaire d'un gradient de protons. Chez les plantes supérieures, de nombreux champignons et quelques protistes, une seconde voie de transfert diverge de la voie principale au niveau du pool d'ubiquinone, celui-ci étant alors oxydé directement par l'oxygène moléculaire. Lorsque les électrons empruntent cette voie alternative, deux sites d'éjection de protons sont court-circuités et l'énergie produite est dissipée sous forme de chaleur. Cette réaction est catalysée par une enzyme unique, l'oxydase alternative (AOX), souvent encodée par une petite famille multigénique chez les plantes supérieures. Une activité accrue de la voie alternative est observée suite à divers stimuli développementaux et environnementaux, en particulier en conditions de stress. Cette augmentation d'activité résulte d'une activation transcriptionnelle du gène Aox et/ou de modifications post-traductionnelles de la protéine mature. L'AOX de l'algue verte unicellulaire Chlamydomonas reinhardtii est encodée par deux gènes différents, Aox1 et Aox2, le premier étant beaucoup plus transcrit que le second. Les cDNAs Aox1 et Aox2, de même que la séquence génomique Aox2, ont été isolés et caractérisés dans notre laboratoire. Dans un premier temps, nous avons entrepris le clonage et la caractérisation de la séquence génomique Aox1, ce qui nous a permis de comparer sa structure avec celle de son homologue Aox2. Ensuite, afin d'étudier sa régulation transcriptionnelle, nous avons fusionné un segment de 1,4 kb contenant la région promotrice Aox1 à la région codante du gène (Ars) de l'arylsulfatase et mesuré les activités ARS dans des transformants porteurs de la construction chimérique. Nous avons ainsi montré que le promoteur Aox1 est insensible à la plupart des inducteurs classiques de l'AOX, parmi lesquels des agents de stress, des inhibiteurs respiratoires et des métabolites. En revanche, l'expression du gène Aox1 répond à la nature de la source d'azote, sa transcription étant réprimée par l'ammonium et stimulée par le nitrate. De plus, en milieu contenant du nitrate, l'inactivation de la nitrate réductase (première enzyme de la voie d'assimilation du nitrate) conduit à une expression du gène Aox1 encore plus importante. Nous avons en outre observé que cette stimulation par le nitrate se répercute aux niveaux protéique et respiratoire. Une étude de délétion de la région promotrice Aox1 indique qu'un segment court (de −253 à +59 par rapport à l'origine de transcription) est suffisant pour assurer la transcription et la régulation du gène, mais que son expression maximale requiert également des éléments distaux. Aucun motif nucléotidique susceptible d'intervenir dans l'expression du gène Aox1 n'a été identifié à l'issue d'une analyse bioinformatique du promoteur. L'effet de la nature de la source d'azote sur l'expression de l'AOX est interprété sous l'angle d'une optimisation de la synthèse d'ATP mitochondrial sans modification de l'activité respiratoire, en relation avec un possible accroissement de la production d'ATP photosynthétique lorsque le nitrate est utilisé comme source d'azote. [less ▲]

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See detailClonal chromosome aberrations in Philadelphia-negative cells from chronic myelocytic leukemia patients treated with imatinib mesylate: report of two cases.
Herens, Christian ULg; Baron, Frédéric ULg; Croisiau, Christiane et al

in Cancer Genetics & Cytogenetics (2003), 147(1), 78-80

Imatinib mesylate (tested as STI571), an abl kinase inhibitor, induces sustained, complete hematologic and cytogenetic responses in chronic myelocytic leukemia (CML) patients; however, emergence of clonal ... [more ▼]

Imatinib mesylate (tested as STI571), an abl kinase inhibitor, induces sustained, complete hematologic and cytogenetic responses in chronic myelocytic leukemia (CML) patients; however, emergence of clonal chromosomal aberrations in Philadelphia-negative (Ph-) cells during treatment has been reported. We describe two CML patients in chronic phase who presented with complete cytogenetic responses during imatinib mesylate therapy but developed new clonal chromosomal rearrangements in Ph- cells. The first patient presented with a duplication of chromosome 1, dup(1)(q21q42), and the second showed two new clonal aberrations consisting of inv(1)(q12q32) and del(7)(q22) in the same clone. [less ▲]

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See detailClonal diversity and metallo-beta-lactamase production in clinical isolates of Stenotrophomonas maltophilia
Mercuri, Paola ULg; Ishii, Y.; Ma, L. et al

in Microbial Drug Resistance : Mechanism, Epidemiology, & Disease (2002), 8(3), 193-200

Stenotrophomonas maltophilia is a nosocomial pathogen with an intrinsic broad-spectrum resistance to beta-lactam compounds and other antibacterial agents. It produces two chromosomal beta-lactamases: a ... [more ▼]

Stenotrophomonas maltophilia is a nosocomial pathogen with an intrinsic broad-spectrum resistance to beta-lactam compounds and other antibacterial agents. It produces two chromosomal beta-lactamases: a clavulanic acid-sensitive class A (L2) and a tetrameric carbapenemase (L1 or BlaS). We screened 40 S. maltophilia multidrug-resistant clinical isolates recovered between 1995 and 1998 in the Varese Hospital (Italy) for the presence of the metallo-beta-lactamase. The isolates were investigated by phenotypic profiling (enzymatic activity and antibiotic resistance pattern) and molecular methods such as PCR and pulsed-field gel electrophoresis (PFGE) to reveal intraspecies diversity. For the tested S. maltophilia strains, we showed that the beta-lactamase production could be induced by the presence of imipenem (50 mug/ml) in the culture media. Addition of 1 mM dipicolinic acid completely inhibited the hydrolysis of imipenem but decreased that nitrocefin in a strain-dependent manner. Full activity of crude extract towards imipenem could be restored by addition of 1 mM ZnCl2. Finally, the gene encoding the carbapenem-hydrolyzing beta-lactamase from S. maltophilia ULA-511 and 39/95, a clinical strain, were isolated and sequenced. These two strains have a different profile of multidrug resistance. The two metallo-beta-lactamases were found to be isologous. The difference of sensitivity of these two strains was associated to the level of production of the metallo-beta-lactamase. [less ▲]

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See detailCloned Human Polyomavirus JC Can Transform Human Amnion Cells
Howley, P. M.; Rentier-Delrue, Françoise ULg; Heilman, C. A. et al

in Journal of Virology (1980), 36

The genome of the human polyomavirus JC (Mad-1 strain) was molecularly cloned in Escherichia coli by using the plasmid vector pBR322. Recombinant DNA molecules were constructed with the entire JC genome ... [more ▼]

The genome of the human polyomavirus JC (Mad-1 strain) was molecularly cloned in Escherichia coli by using the plasmid vector pBR322. Recombinant DNA molecules were constructed with the entire JC genome inserted either at its unique EcoRI site at 0.0 map units or at its unique BamHI site at 0.51 map units. Viral DNA from each of these recombinant plasmids was capable of transforming human amnion cells, and cell lines established from transformed foci were positive for JC tumor antigen as assayed by indirect immunofluorescence. [less ▲]

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See detailThe Clones’ Apprenticeship: Ishiguro’s Never Let Me Go as a Bildungsroman
Guesse, Carole ULg

in Anglica : An International Journal of English Studies (2016), 25(1), 155-169

This article considers as a Bildungsroman the 2005 novel Never Let Me Go by Kazuo Ishiguro, which depicts the education of young clones in a boarding school in a 1990s uchronic England. It studies the ... [more ▼]

This article considers as a Bildungsroman the 2005 novel Never Let Me Go by Kazuo Ishiguro, which depicts the education of young clones in a boarding school in a 1990s uchronic England. It studies the main theoretical works about this type of writing in order to isolate some of its defining characteristics and then evaluate the possibility of an analogy between the fictional developments of humans and clones. It concludes that, even though the Bildunsgroman has strong ties with the changing nineteenth-century society, it has been adapted to other – even non-existing – environments. [less ▲]

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See detailClonidine and ketanserin both are effective treatment for postanesthetic shivering.
Joris, Jean ULg; Banache, Maryse; Bonnet, Francis et al

in Anesthesiology (1993), 79(3), 532-9

BACKGROUND: Although meperidine is an effective treatment of postanesthetic shivering, its mechanism of action remains unknown. Investigation of other drugs might help clarify the mechanisms by which ... [more ▼]

BACKGROUND: Although meperidine is an effective treatment of postanesthetic shivering, its mechanism of action remains unknown. Investigation of other drugs might help clarify the mechanisms by which shivering can be controlled. Accordingly, we investigated the efficacy of clonidine, an alpha 2-adrenergic agonist, and ketanserin, a 5-hydroxytryptamine antagonist, in treating postanesthetic shivering. METHODS: First, 54 patients shivering after general anesthesia were allocated randomly to receive an intravenous bolus of saline, 150 micrograms clonidine, or 10 mg ketanserin. A second study explored the dose-dependence of clonidine. Forty shivering patients were given saline or clonidine, 37.5, 75, or 150 micrograms. RESULTS: The duration of shivering was significantly shorter in those given clonidine (2.1 +/- 0.9 min) than in the other two groups and shorter in the ketanserin group (4.3 +/- 0.9 min) than in the saline group (12.0 +/- 1.6 min). Clonidine and ketanserin significantly decreased systolic arterial blood pressure when compared to saline. Core rewarming was significantly slower in the clonidine group. In the second study, 37.5 micrograms clonidine was no more effective than saline. Two minutes after treatment, 150 micrograms obliterated shivering in all patients. Five minutes after treatment, all patients given 75 micrograms had stopped shivering. Systolic arterial pressure and heart rate decreased significantly in patients given 75 and 150 micrograms clonidine. CONCLUSIONS: Clonidine (150 micrograms) and ketanserin (10 mg) both are effective treatment for postanesthetic shivering. The effect of clonidine on shivering is dose-dependent: whereas 37.5 micrograms had no effect, 75 micrograms clonidine stopped shivering within 5 min. [less ▲]

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See detailClonidine test and MMPI scales in major depression
Hansenne, Michel ULg; PITCHOT, William ULg; Gonzalez, Moreno et al

in European Psychiatry (1992), 7

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See detailThe clonidine test in posttraumatic stress disorder.
Hansenne, Michel ULg; Pitchot, William ULg; Ansseau, Marc ULg

in American Journal of Psychiatry (The) (1991), 148(6), 810-1

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See detailCloning and amplified expression in Streptomyces lividans of a gene encoding extracellular β-lactamase from Streptomyces albus G
Dehottay, Philippe; Dusart, Jean; Duez, Colette ULg et al

in Gene (1986), 42(1), 31-36

A 4.9-kb DNA fragment containing the bla gene for the extracellular beta-lactamase (BLA) of Streptomyces albus G was cloned in Streptomyces lividans using the conjugative, low-copy-number plasmid pIJ61 as ... [more ▼]

A 4.9-kb DNA fragment containing the bla gene for the extracellular beta-lactamase (BLA) of Streptomyces albus G was cloned in Streptomyces lividans using the conjugative, low-copy-number plasmid pIJ61 as vector. No expression of bla was observed when this DNA fragment was introduced into Escherichia coli HB101 on a plasmid vector. A 1.5-kb PstI-SstI fragment containing the bla gene was cloned in S. lividans on the nonconjugative, high-copy-number plasmid pIJ702. A tenfold higher yield of BLA was obtained from S. lividans carrying this plasmid than from S. albus G grown under optimal production conditions. The BLA from the clone reacts with beta-iodopenicillanate according to a branched pathway which is characteristic of the original S. albus G BLA enzyme. [less ▲]

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See detailCloning and amplified expression in Streptomyces lividans of the gene encoding the extracellular β-lactamase from Streptomyces cacaoi
Lenzini, Mauro V; Nojima, Shiego; Dusart, Jean et al

in Journal of General Microbiology (1987), 133(10), 2915-2920

A 19 kb SphI DNA fragment containing the gene for the extracellular active-site serine beta-lactamase of Streptomyces cacaoi KCC-SO352 was cloned in Streptomyces lividans TK24 using the high-copy-number ... [more ▼]

A 19 kb SphI DNA fragment containing the gene for the extracellular active-site serine beta-lactamase of Streptomyces cacaoi KCC-SO352 was cloned in Streptomyces lividans TK24 using the high-copy-number plasmid pIJ702 as vector. A 30-fold higher yield of beta-lactamase was obtained from S. lividans strain ML1, carrying the recombinant plasmid pDML51, than from S. cacaoi grown under optimal production conditions. In all respects (molecular mass, isoelectric point, kinetics of inhibition by beta-iodopenicillanate) the overproduced S. lividans ML1 beta-lactamase was identical to the original S. cacaoi enzyme. A considerable reduction of beta-lactamase production was caused by elimination of a 12.8 kb portion of the 19 kb DNA fragment by cleavage at an internal SphI site located more than 3 kb upstream of the beta-lactamase structural gene. The beta-lactamase gene was located within a 1.8 NcoI-BclI fragment but when this fragment was cloned in S. lividans pIJ702, the resulting strain produced hardly any more beta-lactamase than the original S. cacaoi. [less ▲]

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See detailCloning and amplified expression in Streptomyces lividans of the gene encoding the extracellular β-lactamase of Actinomadura R39
Fraipont, Claudine ULg; Duez, Colette ULg; Matagne, André ULg et al

in Biochemical Journal (1989), 262(3), 849-854

By using the promoter-probe plasmid pIJ424, genomic DNA fragments of Actinomadura R39 were shown to have promoter activity in Streptomyces lividans. The same 100-200-copy-number plasmid was used to clone ... [more ▼]

By using the promoter-probe plasmid pIJ424, genomic DNA fragments of Actinomadura R39 were shown to have promoter activity in Streptomyces lividans. The same 100-200-copy-number plasmid was used to clone in S. lividans TK24, the gene that encodes the Actinomadura R39, β-lactamase. Gene cloning resulted in an amplified expression of the , β lactamase when compared with the amounts of enzyme produced by the original strain (1 mg versus 0.008 mg . litre of culture-1). [less ▲]

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See detailCloning and characterisation of the primary structure of the sheep lymphocyte function-associated antigen-1 alpha subunit
Fett, Thomas ULg; Zecchinon, Laurent ULg; Baise, Etienne ULg et al

in Molecular Immunology (2005), 42(12), 1503-1508

The leukocyte integrins play a critical role in a number of cellular adhesive interactions during the immune response. The ovine cDNA encoding CD1 1a, the predominant a subunit of the beta(2)-integrin ... [more ▼]

The leukocyte integrins play a critical role in a number of cellular adhesive interactions during the immune response. The ovine cDNA encoding CD1 1a, the predominant a subunit of the beta(2)-integrin family, was sequenced and compared with the human, bovine and murine sequences. Despite some focal differences, it shares all the main characteristics of its known mammalian homologues. Along with the ovine CD18-encoding cDNA, which is available for a few months, the sequence data provided here will allow the Ovis aries beta(2)-integrin CD1 1a/CD18 (LEA-1, alpha(L)beta 2) expression in vitro as a tool to examine the specificities of inflammation in the ovine species. (c) 2005 Elsevier Ltd. All rights reserved. [less ▲]

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