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See detailThe complete amino acid sequence of the Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase of streptomyces albus G
Joris, Bernard ULg; Van Beeumen, Jozef; Casagrande, Fabiana et al

in European Journal of Biochemistry (1983), 130(1), 53-69

The 22076-Mr Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase of Streptomyces abuls G effectively catalyses the transfer of the N alpha, N epsilon-diacetyl-L-lysyl-D-alanyl electrophilic group ... [more ▼]

The 22076-Mr Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase of Streptomyces abuls G effectively catalyses the transfer of the N alpha, N epsilon-diacetyl-L-lysyl-D-alanyl electrophilic group of the standard tripeptide substrate N alpha, N epsilon-diacetyl-L-lysyl-D-alanyl-D-alanine to water. It also performs a weak beta-lactamase activity, hydrolysing penicillin into penicilloate at a very low rate. This protein consists of 212 amino acid residues in a single polypeptide chain. The N terminus is partially blocked as a result of the cyclization of the dipeptide Asn-Gly into anhydroaspartylglycine imide. The protein has been fragmented by cyanogen bromide into five fragments whose sequences have been determined via appropriate subcleavages with various proteases. The ordering of the cyanogen bromide peptide fragments has been carried out (a) by submitting the S-carboxymethylated protein to complete tryptic digestion and labelling the methionine-containing peptides thus obtained with iodo[14C]-acetamide, and (b) by submitting to limited tryptic digestion the S-[2-(4'-pyridyl)ethyl]-cysteine protein whose amino groups have been blocked by reaction with exo-cis-3,6-endoxo-delta 4-tetrahydrophthalic anhydride prior to digestion. The protein contains six cysteine residues in the form of three disulfide bridges. No homology is found by comparing this peptidase with other Zn2+-containing enzymes (carboxypeptidase A, thermolysin, carbonic anhydrase B and alcohol dehydrogenase) and several completely or partially sequenced, serine-containing D-alanyl-D-alanine-cleaving peptidases and Zn2+/serine-containing beta-lactamases. [less ▲]

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See detailComplete bifurcation behaviour of aeroelastic systems with freeplay
Dimitriadis, Grigorios ULg

in Proceedings of the 52nd AIAA/ASME/ASCE/AHS/ASC Structures, Structural Dynamics and Materials Conference (2011, April)

Over the last couple of decades, a significant amount of research has been carried out on the aeroelastic behaviour of aeroelastic systems with freeplay. It has been established that such systems can ... [more ▼]

Over the last couple of decades, a significant amount of research has been carried out on the aeroelastic behaviour of aeroelastic systems with freeplay. It has been established that such systems can undergo Limit Cycle Oscillations (LCO), both periodic and aperiodic. It has also been shown that several LCOs can occur at the same flight conditions, depending on initial conditions. A lot of the work has been applied to a pitch-plunge airfoil with a control surface and freeplay in the control rotation spring but, even for this simple model, the complete LCO behaviour has not been calculated. In this work, a combined approach using equivalent linearization, a shooting-based numerical continuation scheme and branch following is used to calculate the full bifurcation behaviour of such a system. It is shown that the primary LCO branches depend on the underlying linear systems but that there are two branching points from which secondary periodic solution branches emanate and wrap themselves around the primary branches. Up to 13 different LCOs can coexist at a single flight condition. The system undergoes Hopf, fold, flip and Neimark-Sacker bifurcations and the proposed solution method can identify and all of them. [less ▲]

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See detailComplete Dna-Sequence Of Yeast Chromosome-Xi
Dujon, Bernard; Esteban, Pf.; Fernandes, L. et al

in Nature (1994), 369(6479),

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See detailA complete fuzzy decision tree technique
Olaru, C.; Wehenkel, Louis ULg

in Fuzzy Sets and Systems (2003), 138(2), 221-254

In this paper, a new method of fuzzy decision trees called soft decision trees (SDT) is presented. This method combines tree growing and pruning, to determine the structure of the soft decision tree, with ... [more ▼]

In this paper, a new method of fuzzy decision trees called soft decision trees (SDT) is presented. This method combines tree growing and pruning, to determine the structure of the soft decision tree, with refitting and backfitting, to improve its generalization capabilities. The method is explained and motivated and its behavior is first analyzed empirically on 3 large databases in terms of classification error rate, model complexity and CPU time. A comparative study on 11 standard UCI Repository databases then shows that the soft decision trees produced by this method are significantly more accurate than standard decision trees. Moreover, a global model variance study shows a much lower variance for soft decision trees than for standard trees as a direct cause of the improved accuracy. (C) 2003 Elsevier B.V. All rights reserved. [less ▲]

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See detailComplete genome sequence of a novel bovine norovirus: Evidence for slow genetic evolution in genogroup III genotype 2 noroviruses
Mauroy, Axel ULg; Scipioni, A.; Mathijs, E. et al

in Journal of Virology (2012), 86(22), 12449-12450

A new genogroup III genotype 2 bovine norovirus, B309/2003/BE, was entirely sequenced and genetically compared to the original Newbury2/1976/UK strain and to Dumfries/1994/UK, detected in 1976 and 1994 ... [more ▼]

A new genogroup III genotype 2 bovine norovirus, B309/2003/BE, was entirely sequenced and genetically compared to the original Newbury2/1976/UK strain and to Dumfries/1994/UK, detected in 1976 and 1994, respectively. Interestingly, except in welldefined coding regions (N-terminal protein, 3A-like protease, hypervariable region of the capsid protein, and C-terminal part of the minor structural protein), very low genetic differences were noted between the entire genomes of these three strains along a 30-year-long period. It allowed some hypotheses of hotspots of genetic evolution through a low genetic evolution background in genotype 2 genogroup III bovine noroviruses. © 2012, American Society for Microbiology. [less ▲]

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See detailThe Complete Genome Sequence Of The Gram-Positive Bacterium Bacillus Subtilis
Kunst, F.; Ogasawara, N.; Moszer, I. et al

in Nature (1997), 390(6657), 249-256

Bacillus subtilis is the best-characterized member of the Gram-positive bacteria. Its genome of 4,214,810 base pairs comprises 4,100 protein-coding genes. Of these protein-coding genes, 53% are ... [more ▼]

Bacillus subtilis is the best-characterized member of the Gram-positive bacteria. Its genome of 4,214,810 base pairs comprises 4,100 protein-coding genes. Of these protein-coding genes, 53% are represented once, while a quarter of the genome corresponds to several gene families that have been greatly expanded by gene duplication, the largest family containing 77 putative ATP-binding transport proteins. In addition, a large proportion of the genetic capacity is devoted to the utilization of a variety of carbon sources, including many plant-derived molecules. The identification of five signal peptidase genes, as well as several genes for components of the secretion apparatus, is important given the capacity of Bacillus strains to secrete large amounts of industrially important enzymes. Many of the genes are involved in the synthesis of secondary metabolites, including antibiotics, that are more typically associated with Streptomyces species. The genome contains at least ten prophages or remnants of prophages, indicating that bacteriophage infection has played an important evolutionary role in horizontal gene transfer, in particular in the propagation of bacterial pathogenesis. [less ▲]

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See detaila complete insect from the Late Devonian period - supplementary information
Garrouste, Romain; Clément, Gaël; Nel, Patricia et al

in Nature (2012)

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See detailA complete insect from the Late Devonian period
Garrouste, Romain; Clément, Gaël; Nel, Patricial et al

in Nature (2012), 488

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See detailComplete Nucleotide Sequence Of Saccharomyces Cerevisiae Chromosome X
Galibert, F.; Alexandraki, D.; Baur, A. et al

in Embo Journal (1996), 15(9),

Detailed reference viewed: 11 (3 ULg)
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See detailA Complete Open-Source Solution for Electromagnetic Field Computation
Geuzaine, Christophe ULg; Dular, Patrick ULg; Remacle, J.-F.

in Proceedings of the 12th IEEE Conference on Electromagnetic Field Computation, CEFC 2006 (2006)

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See detailComplete Resistance to Gonadotropin Hormone Releasing Hormone (Gn-RH) Agonist Therapy for Metastatic Prostate Cancer
Valdes Socin, Hernan Gonzalo ULg; Waltregny, David ULg; Beckers, Albert ULg

in European Neuroendocrine Association - Liège, 22-25 septembre 2010 (2010, September)

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See detailCompletely inelastic ball
Gilet, Tristan ULg; Vandewalle, Nicolas ULg; Dorbolo, Stéphane ULg

in Physical Review. E : Statistical, Nonlinear, and Soft Matter Physics (2009)

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See detailCompletion of biological networks: the output kernel trees approach
Geurts, Pierre ULg; Touleimat, Nizar; Dutreix, Marie et al

in Proceedings of the the Workshop on Probabilistic Modeling and Machine Learning in Structural and Systems Biology (2006)

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See detailA complex anterior mediastinal mass: demonstration of pericardial haemangioma by dynamic MRI (2003:10b).
NCHIMI LONGANG, Alain ULg; Ghaye, B.; Szapiro, D. et al

in European Radiology (2004), 14(1), 160-3

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See detailA complex composed of at least two HeLa nuclear proteins protects preferentially one DNA strand of the simple (gt)n(ga)m containing region of intron 2 in HLA-DRB genes.
Maueler, Winfried; Frank, G.; Muller, Marc ULg et al

in Journal of Cellular Biochemistry (1994), 56(1), 74-85

Electrophoretic mobility shift assays reveal that HeLa nuclear proteins bind fast and with measurable affinity to target DNAs containing mixed simple repetitive (gt)n(ga)m stretches. Preincubation of the ... [more ▼]

Electrophoretic mobility shift assays reveal that HeLa nuclear proteins bind fast and with measurable affinity to target DNAs containing mixed simple repetitive (gt)n(ga)m stretches. Preincubation of the proteins at elevated temperature prevents the formation of the major DNA/protein complex in favour of several distinct assemblies. A similar pattern of retarded bands was observed employing higher salt concentrations in the binding reaction. Thus conformational changes of different proteins appear to influence the complex rather than alternating DNA structures. Separation of the total nuclear extract into a water soluble and an insoluble protein fraction leads to a complete loss of target DNA binding capability of the fractions. The binding capacity is restored by combining the two fractions suggesting that at least two protein components are necessary to form a complex with the target sequence. The proteins can be differentiated into heat sensitive, water soluble and temperature stable, water insoluble, respectively. Furthermore, specifically binding polypeptides are not detectable by Southwestern analyses, probably because the essential components are separated during electrophoresis. DNase I footprint analyses yield four different protein binding regions only on the (gt)n(ga)m harbouring strand. The footprints cover larger portions of the mixed simple repeat in addition to a portion 5' of the (gt)n part. Hence at least two nuclear protein components of unknown biological function have to be present simultaneously to protect preferentially the (gt)n(ga)m-containing strand of intron 2 in HLA-DRB genes. [less ▲]

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See detailComplex dynamics and targeted energy transfer in linear oscillators coupled to multi-degree-of-freedom essentially nonlinear attachments
Tsakirtzis, Stylianos; Panagopoulos, Panagiotis; Kerschen, Gaëtan ULg et al

in Nonlinear Dynamics (2007), 48(3), 285-318

We study the dynamics of a system of coupled linear oscillators with a multi-DOF end attachment with essential (nonlinearizable) stiffness nonlinearities. We show numerically that the multi-DOF attachment ... [more ▼]

We study the dynamics of a system of coupled linear oscillators with a multi-DOF end attachment with essential (nonlinearizable) stiffness nonlinearities. We show numerically that the multi-DOF attachment can passively absorb broadband energy from the linear system in a one-way, irreversible fashion, acting in essence as nonlinear energy sink (NES). Strong passive targeted energy transfer from the linear to the nonlinear subsystem is possible over wide frequency and energy ranges. In an effort to study the dynamics of the coupled system of oscillators, we study numerically and analytically the periodic orbits of the corresponding undamped and unforced hamiltonian system with asymptotics and reduction. We prove the existence of a family of countable infinity of periodic orbits that result from combined parametric and external resonance interactions of the masses of the NES. We numerically demonstrate that the topological structure of the periodic orbits in the frequency-energy plane of the hamiltonian system greatly influences the strength of targeted energy transfer in the damped system and, to a great extent, governs the overall transient damped dynamics. This work may be regarded as a contribution towards proving the efficacy the utilizing essentially nonlinear attachments as passive broadband boundary controllers. [less ▲]

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See detailComplex dynamics of a linear oscillator with a nonlinear attachment
Lee, Young Sup; Kerschen, Gaëtan ULg; Vakakis, Alexander F. et al

in Fifth EUROMECH Nonlinear Dynamics Conference, Eindhoven, 2005 (2005, August)

We study the dynamics of a two-degree-of-freedom (DOF) nonlinear system consisting of a grounded linear oscillator coupled to a light mass by means of an essentially nonlinear (nonlinearizable) stiffness ... [more ▼]

We study the dynamics of a two-degree-of-freedom (DOF) nonlinear system consisting of a grounded linear oscillator coupled to a light mass by means of an essentially nonlinear (nonlinearizable) stiffness. We show that the structure of periodic orbits of the undamped system greatly influences the damped dynamics, as it causes complicated transitions between modes in the damped transient motion. In addition, there is the possibility of strong passive energy transfer (energy pumping) from the linear oscillator to the nonlinear attachment if certain periodic orbits of the undamped dynamics are excited by the initial conditions.. [less ▲]

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See detailComplex dynamics of a nonlinear aerospace structure: experimental identification and modal interactions
Noël, Jean-Philippe ULg; Renson, Ludovic ULg; Kerschen, Gaëtan ULg

in Journal of Sound & Vibration (2014), 333

Nonlinear system identification is a challenging task in view of the complexity and wide variety of nonlinear phenomena. The present paper addresses the identification of a real-life aerospace structure ... [more ▼]

Nonlinear system identification is a challenging task in view of the complexity and wide variety of nonlinear phenomena. The present paper addresses the identification of a real-life aerospace structure possessing a strongly nonlinear component with multiple mechanical stops. The complete identification procedure, from nonlinearity detection and characterization to parameter estimation, is carried out based upon experimental data. The combined use of various analysis techniques, such as the wavelet transform and the restoring force surface method, brings different perspectives to the dynamics. Specifically, the structure is shown to exhibit particularly interesting nonlinear behaviors, including jumps, modal interactions, force relaxation and chattering during impacts on the mechanical stops. [less ▲]

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