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See detailCharacterization of menin from normal individuals and MEN1 patients
Poncin, Jacques ULg; Closset, J.; Legros, J. J. et al

in 6th International Pituitary Congress - Abstract book (1999)

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See detailCharacterization of methicilin-resistant Staphylococcus pseudintermedius isolated from dogs and cats
Bardiau, M; Yamazaki, K; Ote, I et al

in Microbiology and Immunology (2013), 57

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See detailCharacterization of methicillin-resistant Staphylococcus pseudintermedius isolated from dogs and cats
Bardiau, Marjorie ULg; Yamazaki, Kazuko; Ote, Isabelle et al

in Microbiology and Immunology (2013)

Detailed reference viewed: 37 (7 ULg)
See detailCharacterization of modern Prussian blue pigments by Mössbauer spectroscopy and synchrotron radiation
Samain, Louise ULg; Silversmit, Geert; Sougrati, Moulay T. et al

Conference (2009, November 27)

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See detailCharacterization of monoclonal antibodies against Hemolysin Alpha of Staphylococcus aureus
Crasson, Oscar ULg; Rhazi, Noureddine; Kinet, Marie et al

Poster (2013, February 26)

In this study, vaccine-based targeting of Hemolysin Alpha (HLA) provides protection against lethal staphylococcal infection in a murine model system, suggesting that a monoclonal antibody-based therapy ... [more ▼]

In this study, vaccine-based targeting of Hemolysin Alpha (HLA) provides protection against lethal staphylococcal infection in a murine model system, suggesting that a monoclonal antibody-based therapy may likewise prove to be efficacious for prevention and treatment of this infection. We report the generation of four distinct anti-α-hemolysin monoclonal antibodies (MAbs) that antagonize toxin activity in vitro and protecting experimental animals against lethal S. aureus infection. [less ▲]

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See detailCharacterization of monoclonal antibodies against stromelysin-3 and their use to evaluate stromelysin-3 levels in breast carcinoma by semi-quantitative immunohistochemistry
Santavicca, M.; Noël, Agnès ULg; Chenard, M. P. et al

in International Journal of Cancer = Journal International du Cancer (1995), 64(5), 336-341

Stromelysin-3 (ST3) is a matrix metalloproteinase which is expressed in fibroblastic cells of most human invasive carcinomas and represents a potential new prognostic indicator. Expression of recombinant ... [more ▼]

Stromelysin-3 (ST3) is a matrix metalloproteinase which is expressed in fibroblastic cells of most human invasive carcinomas and represents a potential new prognostic indicator. Expression of recombinant ST3 forms in Escherichia coli from cDNA constructs indicated that high levels of expression were achieved when the ST3 pro-domain was deleted. The putative mature form of ST3 thus produced and recovered from bacterial inclusion bodies was used to prepare monoclonal antibodies (MAbs) against ST3 by immunization of BALB/C mice. Ten hybridomas producing MAbs against ST3 were obtained and analyzed for their ability to detect endogenous ST3 in breast cancer and in conditioned media from human fibroblasts. One of these MAbs (5ST-4A9) was found to be suitable for the routine detection of ST3 on breast cancer tissue sections, thus opening the possibility to evaluate ST3 prognostic value in breast cancer using semi-quantitative immunohistochemistry [less ▲]

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See detailCharacterization of Monoclonal Antibodies to Bovine Viral Diarrhoea Virus: Evidence of a Neutralizing Activity against Gp48 in the Presence of Goat Anti-Mouse Immunoglobulin Serum
Boulanger, D.; Waxweiler, Sophie; Karelle, L. et al

in Journal of General Virology (1991), 72(5), 1195-1198

Twenty-one monoclonal antibodies (MAbs) directed against the NY-1 and the Osloss-c strains of bovine viral diarrhoea virus were produced and characterized by indirect immunofluorescence assay ... [more ▼]

Twenty-one monoclonal antibodies (MAbs) directed against the NY-1 and the Osloss-c strains of bovine viral diarrhoea virus were produced and characterized by indirect immunofluorescence assay, radioimmunoprecipitation and neutralization tests. Fourteen MAbs directed against the NY-1 strain recognized the gp48 and showed a weak neutralizing activity in the presence of goat anti-mouse immunoglobulin serum. [less ▲]

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See detailCharacterization of monovarietal virgin olive oils from introduced cultivars in eastern Morocco
Mansouri, Farid ULg; Benmoumen, A.; Richard, Gaetan ULg et al

in Rivista Italiana Sostanze Grasse (2016), 93

The aim of this study was to characterize monovarietal virgin olive oils (VOOs) of new high-density planting system of three European cultivars (Arbequina, Arbosana and Koroneiki), recently introduced in ... [more ▼]

The aim of this study was to characterize monovarietal virgin olive oils (VOOs) of new high-density planting system of three European cultivars (Arbequina, Arbosana and Koroneiki), recently introduced in eastern Morocco. VOOs’ characterization has been carried out by analyzing several parameters, such as quality indexes, fatty acid contents, minor components, and olive oils’ oxidative stability index (OSI). In this study, we have also conducted a comparison between these monovarietal VOOs and olive oils of autochthones cultivar Picholine marocaine. Significant differences between the analyzed VOOs were highlighted. Koroneiki’s VOO had a high phenols content (493.66 mg/kg) and, consequently, the best oxidative stability (94.83 h); Arbrosana’s VOO was distinguished by its abundance of α-tocopherol (460.07 mg/kg) and by an intermediate OSI (64.83 h). In addition, results showed, firstly, that in all the analyzed oils decarboxymethyl ligstroside aglycone and decarboxymethyl oleuropein aglycone were the main phenolic compounds, and, secondly, that VOOs of Koroneiki and Arbosana seem to have similar profiles, with a high content of natural antioxidants and a high oleic/linoleic ratio, thus boasting a better shelf life. [less ▲]

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See detailCharacterization of multi-walled carbon nanotube dispersion in resorcinol-formaldehyde aerogel
Haghgoo, Majid; Yousefi, Ali Akbar; Zohouriaan Mehr, Mohammad Jalal et al

in Microporous and Mesoporous Materials (2014), 184

Detailed reference viewed: 37 (1 ULg)
See detailCharacterization of multi-walled carbon nanotubes by image analysis
Gommes, Cédric ULg; Bossuot, Christophe ULg; Pirard, Jean-Paul ULg

Scientific conference (2002, October)

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See detailA characterization of multidimensional S-automatic sequences
Charlier, Emilie ULg; Kärki, Tomi ULg; Rigo, Michel ULg

in Actes des rencontres du CIRM, 1 (2009)

An infinite word is S-automatic if, for all n ≥ 0, its (n + 1)st letter is the output of a deterministic automaton fed with the representation of n in the considered numeration system S. In this extended ... [more ▼]

An infinite word is S-automatic if, for all n ≥ 0, its (n + 1)st letter is the output of a deterministic automaton fed with the representation of n in the considered numeration system S. In this extended abstract, we consider an analogous definition in a multidimensional setting and present the connection to the shape-symmetric infinite words introduced by Arnaud Maes. More precisely, for d ≥ 2, we state that a multidimensional infinite word x : N^d → \Sigma over a finite alphabet \Sigma is S-automatic for some abstract numeration system S built on a regular language containing the empty word if and only if x is the image by a coding of a shape-symmetric infinite word. [less ▲]

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See detailCharacterization of mycosubtilin, a natural antifungal agent, in membrane biomimetic systems
Nasir, Mehmet Nail ULg; Kouzayha, Achraf; Wattraint, Olivier et al

Poster (2008)

The emergence of several diseases which affects immune system (HIV infections, few cancers) and the expansion of immunosuppressive and chemical anticancer therapies increase mycosis frequency. Treatments ... [more ▼]

The emergence of several diseases which affects immune system (HIV infections, few cancers) and the expansion of immunosuppressive and chemical anticancer therapies increase mycosis frequency. Treatments currently used to cure mycoses meet problems such as antifungal agent toxicity or pathogenic strain resistance. Thus there is increasing interest for new medical applications of antifungal molecules. In this context, we investigated the behaviour of an antifungal lipopeptide, mycosubtilin, on cytoplasmic membranes. In this aim, biomimetic systems of biological membranes, such as lipid monolayers at the air-water interface and lipid multilayers were used. The interactions of mycosubtilin with these biomimetic systems were examined by different methods (kinetic isotherms, Brewster angle microscopy, FTIR spectroscopy and 1H HR- Magic Angle Spinning NMR). We demonstrated an original behaviour of mycosubtilin in the presence of sterol monolayer, suggesting a mycosubtilin-sterol affinity. We showed as well that mycosubtilin can interact with C=O ester residues and alkyl chains of phospholipids. Moreover, mycosubtilin has a preference for condensed phospholipid domains. [less ▲]

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See detailCharacterization of nanometer-scale roughness in SBA-15 mesoporous silica using image analysis of electron tomograms
Friedrich, Heiner; Gommes, Cédric ULg; de Jongh, Petra et al

Conference (2009, September)

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See detailCharacterization of Nappe Vibration on a Weir
Lodomez, Maurine ULg; Pirotton, Michel ULg; Dewals, Benjamin ULg et al

in Sustainable Hydraulics in the Era of Global Change (2016)

The nappe vibration phenomenon may affect numerous prototype structures, such as gates and spillways, when they operate under low head conditions. This phenomenon, which is far not well understood and ... [more ▼]

The nappe vibration phenomenon may affect numerous prototype structures, such as gates and spillways, when they operate under low head conditions. This phenomenon, which is far not well understood and poorly controlled, produces frequency oscillations in the thin flow nappe cascading downstream of the weir. These oscillations result in a disturbing noise production that increases the environmental and societal impacts of the hydraulic structure. Given limited information available regarding the physical processes of this phenomenon, a detailed investigation has been undertaken to characterize the flow for free-overfall structures where nappe vibration may be of concern. The research conducted on a prototype-scale linear weir model (weir length of 3.5 m and a fall height of 3 m) at the Engineering Hydraulics laboratory of the University of Liège enable to describe the flow and nappe vibration by using image and sound analysis. The paper presents the first quantitative results of the study. [less ▲]

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See detailCharacterization of Nest Sites of Chimpanzees (Pan troglodytes schweinfurthii) in Kibira National Park, Burundi
Hakizimana, Dismas; Hambuckers, Alain ULg; Brotcorne, Fany ULg et al

in African Primates (2015), 10

Abstract: Kibira National Park is the only site in Burundi that harbors a large number of chimpanzees (Pan troglodytes schweinfurthii). While information on factors influencing the selection of nest sites ... [more ▼]

Abstract: Kibira National Park is the only site in Burundi that harbors a large number of chimpanzees (Pan troglodytes schweinfurthii). While information on factors influencing the selection of nest sites by chimpanzees is available for other locations of the species’ range, this information is lacking for Kibira National Park. This is mainly due to the political troubles that prevailed in the country from 1993 until 2007, making study there difficult. To better protect this chimpanzee population, it is crucial to survey nest sites to identify the tree species, physical characteristics of the trees and habitat type that chimpanzees preferentially use for nesting. Therefore, in this study, we investigated: 1) the tree species used by chimpanzees for building their nests; 2) nest tree availability in the study area; 3) whether chimpanzee selection of a nest tree is based on physical characteristics such as diameter at breast height, lowest branch height, tree size and crown height; and 4) whether chimpanzees choose their nest sites according to topography and canopy types. We collected data monthly along 16 transects of 3 km each, from September 2011 to February 2013 (18 months). However, data related to the measurements of nests and nest trees were collected for only the last 12 months, from March 2012 to February 2013. We identified tree species used for nesting, and measured physical characteristics of trees used as opposed to surrounding trees unused. The results showed that chimpanzees select certain tree species to build their nests. Among the 32 species of trees bearing nests, chimpanzees used 12 species significantly more frequently than expected and 11 species significantly less frequently than expected. In addition, trees bearing nests were significantly larger and taller than the surrounding trees and had higher lowest branch and bigger canopies. [less ▲]

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See detailCharacterization of new bacterial glycoside hydrolases isolated from agricultural soils using a functional metagenomic approach
Biver, Sophie ULg; Dubois, Benjamin; Stroobants, Aurore ULg et al

Poster (2013, June 10)

Microorganisms play key roles in soil ecosystem functioning, notably through their ability to degrade plant cell wall polymers. For this, bacteria and fungi produce various enzymes such as cellulases ... [more ▼]

Microorganisms play key roles in soil ecosystem functioning, notably through their ability to degrade plant cell wall polymers. For this, bacteria and fungi produce various enzymes such as cellulases, xylanases, glucosidases, esterases or laccases. Finding new enzymes hydrolyzing cellulose, hemicellulose or lignin is not only interesting for a better understanding of the roles of the soil microflora still largely unknown but these enzymes are also useful for various biotechnological applications such as the production of renewable energy from lignocellulosic material. So here, we used a functional metagenomic approach to isolate new bacterial β-glucosidases, which were then biochemically characterized. The new enzymes were identified by functional analysis of agricultural-soil metagenomic libraries hosted in Escherichia coli and screened on medium containing esculin. After sequence analysis and preliminary estimation of the activity of the new β-glucosidases using p-nitrophenol derivatives on intact bacterial cells, the coding sequences of three of them were cloned into a bacterial expression vector so as to overproduce and purify them by affinity chromatography. The chosen enzymes show only 52-64% sequence identity to known family 3 (GH3) or 1 (GH1) glycoside hydrolases of different phyla (Actinobacteria, Acidobacteria and Proteobacteria). Analysis of the E. coli cells expressing each of them revealed that both GH1 proteins (ASEsc9 and ASEsc10) are thermophilic enzymes more active at mildly acidic to neutral pH while the GH3 enzyme (ASEsc6) is an alkaline, mesophilic, β-glucosidase also displaying xylosidase activity. Their coding sequences have been cloned in fusion with a carboxy-terminal His-tag and placed under the control of the IPTG-inducible promoter of the pET-30b vector. The proteins will be overproduced and purified for further characterization. [less ▲]

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See detailCharacterization of new fluorescent siderophores produced by Pseudomonas sp.
Jacques, Philippe ULg; Hautot, D.; Roblain, D. et al

Poster (1989, May)

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See detailCharacterization of non pigmented B16 melanoma cell-derived cytotoxic factors.
Siwek, B.; De Pauw-Gillet, Marie-Claire ULg; Quetin-Leclercq, J. et al

in Chemico-Biological Interactions (1997), 103(1), 59-73

We analyzed and tried to characterize substance(s) responsible for cytotoxic activities detected in culture media conditioned by non pigmented B16 melanoma cells (NPB16). The different cytological tests ... [more ▼]

We analyzed and tried to characterize substance(s) responsible for cytotoxic activities detected in culture media conditioned by non pigmented B16 melanoma cells (NPB16). The different cytological tests used showed that ultrafiltrated conditioned media (CM U1 fraction) contained several cytotoxic factors with a Mw lower than 1000 Da. These factors seemed to act either directly or indirectly on cell membranes, mitochondria, on the cell cycle and on protein and DNA synthesis. A cytotoxic activity could be found even after high dilution of CM U1. These cytotoxic factors were rapidly released by B16 cells in culture, independently of cell confluence. Their activities in the treated cells were also very fast and the cytotoxic effects were irreversible after only a few hours of treatment. These factors were not intermediate products during melanogenesis, neither polyamines, nor proteases. At least one of them seemed to be a small acidic and basic stable peptide without disulfide bounds but not heat stable. The synthesis of at least one of these cytotoxic factors was inhibited by cycloheximide and the cytotoxic activity was partially destroyed by pronase and trypsin, but not by pepsin. The cytotoxicity was not modified by copper complexants or free radical inhibitors (bovine serum albumin (BSA), tyrosine, superoxyde dismutase (SOD), catalase, vitamin E). Furthermore the levels of glutathione peroxydase activity and reduced glutathione did not change after treatment by CM U1 as compared to controls. [less ▲]

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See detailCharacterization of nonlinear aeroservoelastic behaviour
Dimitriadis, Grigorios ULg; Cooper, Jonathan Edware

in RTO-MP-036 Structural Aspects of Flexible Aircraft Control (1999, October)

The characterisation of the behaviour of nonlinear aeroelastic systems has become a very important re- search topic. Nevertheless, most of the work carried out to date concerns the development of unsteady ... [more ▼]

The characterisation of the behaviour of nonlinear aeroelastic systems has become a very important re- search topic. Nevertheless, most of the work carried out to date concerns the development of unsteady CFD solutions in the transonic region. Important though this work is, there is also a need for research which aims at understanding the behaviour of non- linear systems, particularly the occurance of Limit Cycle Oscillations (LCOs). The purpose of this pa- per is to study the stability of a simple aeroservoelas- tic system with nonlinearities in the control system. The work considers both structural and control law nonlinearities and assesses the stability of the system response by use of bifurcation diagrams. It is shown that simple feedback systems designed to increase the stability of the linearised system also stabilise the nonlinear system, although their effects can be less pronounced. Additionally, a nonlinear control law designed to limit the control surface pitch response was found to increase the flutter speed considerably by forcing the system to undergo limit cycle oscilla- tions instead of fluttering. Finally, friction was found to affect the damping of the system but not its sta- bility, as long as the amplitude of the frictional force is low enough not to cause stoppages in the motion. [less ▲]

Detailed reference viewed: 24 (5 ULg)