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See detailCharacterization of lactogen receptor-binding site 1 of human prolactin
Kinet, Sandrina; Goffin, Vincent; Mainfroid, Véronique et al

in Journal of Biological Chemistry (1996), 271(24), 14353-60

Prolactin (PRL) binds to two molecules of PRL receptor (PRLR) through two regions referred to as binding sites 1 and 2. Although binding site 1 has been generally assigned to the pocket delimited by helix ... [more ▼]

Prolactin (PRL) binds to two molecules of PRL receptor (PRLR) through two regions referred to as binding sites 1 and 2. Although binding site 1 has been generally assigned to the pocket delimited by helix 1, helix 4, and the second half of loop 1, the residues involved in receptor binding have not yet all been precisely identified. In an earlier alanine-scanning mutational study, we identified three major binding determinants in loop 1 of human PRL (hPRL) (Goffin, V., Norman, M. & Martial, J. A.(1992) Mol. Endocrinol. 6, 1381-1392). Here we focus on the two other regions that form binding site 1, namely helices 1 and 4. Putative binding residues, selected on the basis of a three-dimensional model of hPRL constructed in this laboratory, were mutated to alanine, and recombinant hPRL mutants produced in Escherichia coli were tested for their ability to bind to the PRLR and to stimulate Nb2 cell proliferation. We thus identified nine single mutations (three in helix 1 and six in helix 4) whose effect was to reduce both binding and mitogenic activity by more than half as compared with wild-type hPRL, indicating the functional involvement of the corresponding residues. Adding these to the three binding determinants identified in loop 1, we now propose a complete picture of PRLR-binding site 1 of hPRL. As we earlier hypothesized, the binding site 1 determinants of hPRL differ from those of human growth hormone, a hPRL homolog. [less ▲]

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See detailCharacterization of marine CaCO3 particles in the context of Global Change
Aerts, Katrien; Godoi, Ricardo; Harlay, Jérôme ULg et al

Poster (2003, January 07)

The expected increase in greenhouse gasses concentrations as a result of human activity is leading to significant climate change in the coming years. The fate of the anthropogenic CO2 has been intensively ... [more ▼]

The expected increase in greenhouse gasses concentrations as a result of human activity is leading to significant climate change in the coming years. The fate of the anthropogenic CO2 has been intensively studied. Being the largest reservoir of reactive carbon, the ocean acts as an important sink for anthropogenic CO2 and plays a significant role on the global biogeochemical cycle of carbon and its perturbations. There remain, however, large uncertainties concerning the uptake of carbon by the ocean, mainly due to insufficient knowledge of processes controlling the carbonate chemistry in surface waters. The effects of precipitation of calcium carbonate by calcifying organisms in the euphotic zone and redissolution of their skeletons have not been fully taken into account. This precipitation-dissolution process affects both the concentration of dissolved inorganic carbon and of total alkalinity and plays thus a significant role in the buffering capacity of seawater and its potential to act as a sink or a source of CO2 for the atmosphere. We aim to study the processes associated with the oceanic production and dissolution of CaCO3 in order to quantify the role of calcifying phytoplanktonic organisms in sequestering CO2. The calcareous skeletons of Coccolithophores, which comprise one of the main groups of calcifying organisms in the photic zone of the ocean, are analysed. The gut content of copepods, which grazes on coccolithophores is examined by different methods like Electron Probe Micro-Analysis (EPMA) and Micro X-Ray Fluorescence (M-XRF) in order to detect the possible dissolution features at the calcite surface. Automatic EPMA is used for the characterization of individual particles from ocean samples for their composition, morphology and size. Subsequent statistical processing techniques classifies the particles into specific particle clusters. [less ▲]

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See detailCharacterization of material for cement production in Mbuji-Mayi area (DR Congo)
Matamba Jibikila, Raphaël ULg; Pirard, Eric ULg

in ACCTA johannesburg 2013 (2013)

Although the City of Mbuji-Mayi (DR Congo) is rich in limestone and clay deposits, cement and other building materials are still being imported sometimes by plane from Kinshasa, Zambia and Tanzania. A ... [more ▼]

Although the City of Mbuji-Mayi (DR Congo) is rich in limestone and clay deposits, cement and other building materials are still being imported sometimes by plane from Kinshasa, Zambia and Tanzania. A consequence of this situation is low consumption of cement and concrete (less than 1kg per capita) and a lack of modern and sustainable infrastructures. Our study provides a qualitative assessment of all cement raw materials available in the region, including limestone and clay in order to produce hydraulic binder with optimized technical and environmental performances. Chemical and mineralogical characterizations were done using XRF spectroscopy and X-ray diffraction (XRD). Thermal analysis, electron microscopy, and mechanical characterizations are planned to assess the performance of the concrete and mortar manufactured with the binder produced. Finally, the ultimate goal is to produce a local hydraulic binder whose physical and mechanical characteristics are similar to those of Portland cement, but whose production is expected to consume less energy and emit less CO2. [less ▲]

Detailed reference viewed: 59 (2 ULg)
See detailCharacterization of menin from leucocytes of normal and men 1-affected individuals
Poncin, J.; Closset, J.; Legros, J.-J. et al

in 7th International workshop on Multiple Endocrine Neoplasia - Abstract book (1999)

Detailed reference viewed: 8 (2 ULg)
See detailCharacterization of menin from leucocytes of normal and men-1 affected individuals
Poncin, Jacques ULg; Closset, Jean ULg; Legros, Jean-Jacques ULg et al

in 81st Annual Meeting of the Endocrine society - Abstract book (1999)

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See detailCharacterization of menin from normal individuals and MEN1 patients
Poncin, Jacques ULg; Closset, J.; Legros, J. J. et al

in 6th International Pituitary Congress - Abstract book (1999)

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See detailCharacterization of methicilin-resistant Staphylococcus pseudintermedius isolated from dogs and cats
Bardiau, M; Yamazaki, K; Ote, I et al

in Microbiology and Immunology (2013), 57

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See detailCharacterization of methicillin-resistant Staphylococcus pseudintermedius isolated from dogs and cats
Bardiau, Marjorie ULg; Yamazaki, Kazuko; Ote, Isabelle et al

in Microbiology and Immunology (2013)

Detailed reference viewed: 37 (7 ULg)
See detailCharacterization of modern Prussian blue pigments by Mössbauer spectroscopy and synchrotron radiation
Samain, Louise ULg; Silversmit, Geert; Sougrati, Moulay T. et al

Conference (2009, November 27)

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See detailCharacterization of monoclonal antibodies against Hemolysin Alpha of Staphylococcus aureus
Crasson, Oscar ULg; Rhazi, Noureddine; Kinet, Marie et al

Poster (2013, February 26)

In this study, vaccine-based targeting of Hemolysin Alpha (HLA) provides protection against lethal staphylococcal infection in a murine model system, suggesting that a monoclonal antibody-based therapy ... [more ▼]

In this study, vaccine-based targeting of Hemolysin Alpha (HLA) provides protection against lethal staphylococcal infection in a murine model system, suggesting that a monoclonal antibody-based therapy may likewise prove to be efficacious for prevention and treatment of this infection. We report the generation of four distinct anti-α-hemolysin monoclonal antibodies (MAbs) that antagonize toxin activity in vitro and protecting experimental animals against lethal S. aureus infection. [less ▲]

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See detailCharacterization of monoclonal antibodies against stromelysin-3 and their use to evaluate stromelysin-3 levels in breast carcinoma by semi-quantitative immunohistochemistry
Santavicca, M.; Noël, Agnès ULg; Chenard, M. P. et al

in International Journal of Cancer = Journal International du Cancer (1995), 64(5), 336-341

Stromelysin-3 (ST3) is a matrix metalloproteinase which is expressed in fibroblastic cells of most human invasive carcinomas and represents a potential new prognostic indicator. Expression of recombinant ... [more ▼]

Stromelysin-3 (ST3) is a matrix metalloproteinase which is expressed in fibroblastic cells of most human invasive carcinomas and represents a potential new prognostic indicator. Expression of recombinant ST3 forms in Escherichia coli from cDNA constructs indicated that high levels of expression were achieved when the ST3 pro-domain was deleted. The putative mature form of ST3 thus produced and recovered from bacterial inclusion bodies was used to prepare monoclonal antibodies (MAbs) against ST3 by immunization of BALB/C mice. Ten hybridomas producing MAbs against ST3 were obtained and analyzed for their ability to detect endogenous ST3 in breast cancer and in conditioned media from human fibroblasts. One of these MAbs (5ST-4A9) was found to be suitable for the routine detection of ST3 on breast cancer tissue sections, thus opening the possibility to evaluate ST3 prognostic value in breast cancer using semi-quantitative immunohistochemistry [less ▲]

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See detailCharacterization of Monoclonal Antibodies to Bovine Viral Diarrhoea Virus: Evidence of a Neutralizing Activity against Gp48 in the Presence of Goat Anti-Mouse Immunoglobulin Serum
Boulanger, D.; Waxweiler, Sophie; Karelle, L. et al

in Journal of General Virology (1991), 72(5), 1195-1198

Twenty-one monoclonal antibodies (MAbs) directed against the NY-1 and the Osloss-c strains of bovine viral diarrhoea virus were produced and characterized by indirect immunofluorescence assay ... [more ▼]

Twenty-one monoclonal antibodies (MAbs) directed against the NY-1 and the Osloss-c strains of bovine viral diarrhoea virus were produced and characterized by indirect immunofluorescence assay, radioimmunoprecipitation and neutralization tests. Fourteen MAbs directed against the NY-1 strain recognized the gp48 and showed a weak neutralizing activity in the presence of goat anti-mouse immunoglobulin serum. [less ▲]

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See detailCharacterization of monovarietal virgin olive oils from introduced cultivars in eastern Morocco
Mansouri, Farid ULg; Benmoumen, A.; Richard, Gaetan ULg et al

in Rivista Italiana Sostanze Grasse (2016), 93

The aim of this study was to characterize monovarietal virgin olive oils (VOOs) of new high-density planting system of three European cultivars (Arbequina, Arbosana and Koroneiki), recently introduced in ... [more ▼]

The aim of this study was to characterize monovarietal virgin olive oils (VOOs) of new high-density planting system of three European cultivars (Arbequina, Arbosana and Koroneiki), recently introduced in eastern Morocco. VOOs’ characterization has been carried out by analyzing several parameters, such as quality indexes, fatty acid contents, minor components, and olive oils’ oxidative stability index (OSI). In this study, we have also conducted a comparison between these monovarietal VOOs and olive oils of autochthones cultivar Picholine marocaine. Significant differences between the analyzed VOOs were highlighted. Koroneiki’s VOO had a high phenols content (493.66 mg/kg) and, consequently, the best oxidative stability (94.83 h); Arbrosana’s VOO was distinguished by its abundance of α-tocopherol (460.07 mg/kg) and by an intermediate OSI (64.83 h). In addition, results showed, firstly, that in all the analyzed oils decarboxymethyl ligstroside aglycone and decarboxymethyl oleuropein aglycone were the main phenolic compounds, and, secondly, that VOOs of Koroneiki and Arbosana seem to have similar profiles, with a high content of natural antioxidants and a high oleic/linoleic ratio, thus boasting a better shelf life. [less ▲]

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See detailCharacterization of multi-walled carbon nanotube dispersion in resorcinol-formaldehyde aerogel
Haghgoo, Majid; Yousefi, Ali Akbar; Zohouriaan Mehr, Mohammad Jalal et al

in Microporous and Mesoporous Materials (2014), 184

Detailed reference viewed: 37 (1 ULg)
See detailCharacterization of multi-walled carbon nanotubes by image analysis
Gommes, Cédric ULg; Bossuot, Christophe ULg; Pirard, Jean-Paul ULg

Scientific conference (2002, October)

Detailed reference viewed: 17 (2 ULg)
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See detailA characterization of multidimensional S-automatic sequences
Charlier, Emilie ULg; Kärki, Tomi ULg; Rigo, Michel ULg

in Actes des rencontres du CIRM, 1 (2009)

An infinite word is S-automatic if, for all n ≥ 0, its (n + 1)st letter is the output of a deterministic automaton fed with the representation of n in the considered numeration system S. In this extended ... [more ▼]

An infinite word is S-automatic if, for all n ≥ 0, its (n + 1)st letter is the output of a deterministic automaton fed with the representation of n in the considered numeration system S. In this extended abstract, we consider an analogous definition in a multidimensional setting and present the connection to the shape-symmetric infinite words introduced by Arnaud Maes. More precisely, for d ≥ 2, we state that a multidimensional infinite word x : N^d → \Sigma over a finite alphabet \Sigma is S-automatic for some abstract numeration system S built on a regular language containing the empty word if and only if x is the image by a coding of a shape-symmetric infinite word. [less ▲]

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See detailCharacterization of mycosubtilin, a natural antifungal agent, in membrane biomimetic systems
Nasir, Mehmet Nail ULg; Kouzayha, Achraf; Wattraint, Olivier et al

Poster (2008)

The emergence of several diseases which affects immune system (HIV infections, few cancers) and the expansion of immunosuppressive and chemical anticancer therapies increase mycosis frequency. Treatments ... [more ▼]

The emergence of several diseases which affects immune system (HIV infections, few cancers) and the expansion of immunosuppressive and chemical anticancer therapies increase mycosis frequency. Treatments currently used to cure mycoses meet problems such as antifungal agent toxicity or pathogenic strain resistance. Thus there is increasing interest for new medical applications of antifungal molecules. In this context, we investigated the behaviour of an antifungal lipopeptide, mycosubtilin, on cytoplasmic membranes. In this aim, biomimetic systems of biological membranes, such as lipid monolayers at the air-water interface and lipid multilayers were used. The interactions of mycosubtilin with these biomimetic systems were examined by different methods (kinetic isotherms, Brewster angle microscopy, FTIR spectroscopy and 1H HR- Magic Angle Spinning NMR). We demonstrated an original behaviour of mycosubtilin in the presence of sterol monolayer, suggesting a mycosubtilin-sterol affinity. We showed as well that mycosubtilin can interact with C=O ester residues and alkyl chains of phospholipids. Moreover, mycosubtilin has a preference for condensed phospholipid domains. [less ▲]

Detailed reference viewed: 62 (1 ULg)
See detailCharacterization of nanometer-scale roughness in SBA-15 mesoporous silica using image analysis of electron tomograms
Friedrich, Heiner; Gommes, Cédric ULg; de Jongh, Petra et al

Conference (2009, September)

Detailed reference viewed: 26 (0 ULg)
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See detailCharacterization of Nappe Vibration on a Weir
Lodomez, Maurine ULg; Pirotton, Michel ULg; Dewals, Benjamin ULg et al

in Sustainable Hydraulics in the Era of Global Change (2016)

The nappe vibration phenomenon may affect numerous prototype structures, such as gates and spillways, when they operate under low head conditions. This phenomenon, which is far not well understood and ... [more ▼]

The nappe vibration phenomenon may affect numerous prototype structures, such as gates and spillways, when they operate under low head conditions. This phenomenon, which is far not well understood and poorly controlled, produces frequency oscillations in the thin flow nappe cascading downstream of the weir. These oscillations result in a disturbing noise production that increases the environmental and societal impacts of the hydraulic structure. Given limited information available regarding the physical processes of this phenomenon, a detailed investigation has been undertaken to characterize the flow for free-overfall structures where nappe vibration may be of concern. The research conducted on a prototype-scale linear weir model (weir length of 3.5 m and a fall height of 3 m) at the Engineering Hydraulics laboratory of the University of Liège enable to describe the flow and nappe vibration by using image and sound analysis. The paper presents the first quantitative results of the study. [less ▲]

Detailed reference viewed: 19 (1 ULg)