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See detailCharacterization of the Sporulation-Related Gamma-D-Glutamyl-(L)Meso-Diaminopimelic-Acid-Hydrolysing Peptidase I of Bacillus Sphaericus NCTC 9602 as a Member of the Metallo(Zinc) Carboxypeptidase A Family. Modular Design of the Protein
Hourdou, Marie-Laure; Guinand, Micheline; Vacheron, Marie-Jeanne et al

in Biochemical Journal (1993), 292(Pt 2), 563-570

The sporulation-related gamma-D-glutamyl-(L)meso-diaminopimelic-acid-hydrolysing peptidase I of Bacillus sphaericus NCTC 9602 has been analysed by proton-induced X-ray emission. It contains 1 equivalent ... [more ▼]

The sporulation-related gamma-D-glutamyl-(L)meso-diaminopimelic-acid-hydrolysing peptidase I of Bacillus sphaericus NCTC 9602 has been analysed by proton-induced X-ray emission. It contains 1 equivalent Zn2+ per mol of protein. As derived from gene cloning and sequencing, the B. sphaericus Zn peptidase I is a two-module protein. A 100-amino-acid-residue N-terminal domain consisting of two tandem segments of similar sequences, is fused to a 296-amino-acid-residue C-terminal catalytic domain. The catalytic domain belongs to the Zn carboxypeptidase A family, the closest match being observed with the Streptomyces griseus carboxypeptidase [Narahashi (1990) J. Biochem. 107, 879-886] and with the family prototype, bovine carboxypeptidase A. The catalytic domain of the B. sphaericus peptidase I possesses, distributed along the amino-acid sequence, peptide segments, a triad His162-Glu165-His307 and a dyad Tyr347-Glu366 that are equivalent to secondary structures, the zinc-binding triad His69-Glu72-His196 and the catalytic dyad Tyr248-Glu270 of bovine carboxypeptidase A respectively. The N-terminal repeats of the B. sphaericus peptidase I have similarity with the C-terminal repeats of the Enterococcus hirae muramidase 2, the Streptococcus (now Enterococcus) faecalis autolysin and the Bacillus phi PZA and phi 29 lysozymes, to which a role in the recognition of a particular moiety of the bacterial cell envelope has been tentatively assigned. Detergents enhance considerably the specific activity of the B. sphaericus peptidase I. [less ▲]

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See detailCharacterization of the transcription start site of the ACTH receptor gene: presence of an intronic sequence in the 5'-flanking region.
Naville, D.; Barjhoux, L.; Jaillard, C. et al

in Molecular and cellular endocrinology (1994), 106(1-2), 131-5

Corticotropin (ACTH) regulates glucocorticoid production through specific receptors on the adrenal cortex. Analysis of the ACTH receptor mRNA in human adrenal has revealed the presence of five transcripts ... [more ▼]

Corticotropin (ACTH) regulates glucocorticoid production through specific receptors on the adrenal cortex. Analysis of the ACTH receptor mRNA in human adrenal has revealed the presence of five transcripts ranging from 1.8 to 11 kilobases (kb). Characterization of the 5'-untranslated regions (UTRs) of the ACTH receptor mRNA demonstrated the presence of one major initiation site of transcription 177 bp away from the ATG codon. Analysis of this 5' sequence showed a perfect alignment with the previously described genomic sequence until position -128 bp from the ATG. The upstream 49-bp sequence was divergent, suggesting the occurrence of a splicing and indicating the presence of an intronic sequence in the UTRs, as well as the presence of an upstream exon containing this 49-bp sequence and located at least 1.8 kb away from the exon encoding the protein. [less ▲]

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See detailCharacterization of the tropospheric small-scale activity
Brenot, Hugues; Warnant, René ULg

Report (2008)

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See detailCharacterization of the Trypsin-Solubilized Penicillin-Binding Proteins of Enterococcus hirae (Streptococcus faecium)
El Kharroubi, Aboubaker; Jacques, Philippe ULg; Piras, Graziella et al

in Actor, Paul; Daneo-Moore, Lolita; Higgins, Michael L. (Eds.) et al Antibiotic Inhibition of Bacterial Cell Surface Assembly and Function (1988)

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See detailCharacterization of the tumor micro-environment after administration of glucocorticoids to understand their radiosensitization effect
Crokart; Jordan; Baudelet et al

Poster (2004, November 26)

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See detailCharacterization of the zebrafish beta-cell transcriptome by RNA-seq
Manfroid, Isabelle ULg; Tarifeno, Estefania; Voz, Marianne ULg et al

Poster (2013, May 27)

The loss of pancreatic insulin-producing cells (beta-cells) is a hallmark of diabetes and more knowledge is needed to find new treatments. Thus, it is crucial to identify novel regulatory genes ... [more ▼]

The loss of pancreatic insulin-producing cells (beta-cells) is a hallmark of diabetes and more knowledge is needed to find new treatments. Thus, it is crucial to identify novel regulatory genes specifically expressed in this pancreatic cell subtype. In the present study, the main pancreatic islet was dissected from transgenic Tg(insulin:GFP) adult zebrafish and beta-cells were selectively recovered by FACS with 98% of purity. Illumina RNA-seq was used to sequence the transcriptome. 20 millions of sequenced reads (paired-end) were obtained, aligned on the zebrafish genome and assembled into transcripts (Tophat/Cufflinks softwares). The zebrafish beta-cells transcriptome includes all known regulatory genes involved in beta-cell differentiation such as pdx1, mnx1, pax6b, neuroD, isl1, insm1, as well as Hopx and Hdac9 genes, both recently identified in human beta-cells. In contrast, the alpha-cell specific transcription factor arx and the acinar marker ptf1a were not detected, confirming the high purity of our beta-cell preparation. Interestingly, many miRNAs were detected, such as dre-mir-375 and dre-mir-7, as well as several lncRNA recently described at embryonic stages. We are currently applying the same approach to the Tg(somatostatin:GFP) and Tg(glucagon:GFP) transgenic lines in to characterize the transcriptome of delta- and alpha-cells. The comparison of these different data will allow us to identify coding and non-coding genes specifically expressed in the different endocrine subtype cells, paving the way for further functional studies. [less ▲]

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See detailCharacterization of the β Phase Decomposition in Ti-5Al-5Mo-5V-3Cr at Slow Heating Rates
Contrepois, Quentin ULg; Carton Marc; Lecomte-Beckers, Jacqueline ULg

in Open Journal of Metal (2011), 1

The influence of slow heating rates: 2, 5, 10 and 30°C/min (0.033, 0.083, 0.166 and 0.50°C/s) on the β phase decomposition of Ti-5Al-5Mo-5V-3Cr (Ti-5553) during continuous heating were characterized by ... [more ▼]

The influence of slow heating rates: 2, 5, 10 and 30°C/min (0.033, 0.083, 0.166 and 0.50°C/s) on the β phase decomposition of Ti-5Al-5Mo-5V-3Cr (Ti-5553) during continuous heating were characterized by differen-tial scanning calorimetry (DSC) analysis, light microscopy, scanning electron microscopy, X-ray diffraction and hardness testing. Starting microstructure was the β phase obtained by heating the Ti-5553 above the Tβ temperature and a water quench. Results show that heating rate has a significant impact on the precipitation mechanisms and on the β→α transformation in this range of heating rates. The main formation of α precipi-tates occurs between 500 and 600°Cat all heating rates tested. A heating at 2°C/min produces very fine and homogeneously distributed α plate precipitates which have nucleated on the nanometer size ωiso precipitates. The ωiso precipitates between 350 and 400°C. At higher heating rates 10, 15 or 30°C/min, the amount of pre-cipitation of ωiso is lower so an additional formation of nanometer size precipitates occurs between 450 and 500°C. It is supposed that both precipitates act as nucleation sites for α phase precipitation. The resultant mi-crostructure consists in a fine intragranular distribution of α precipitates and a coarser precipitation of α at the grain boundaries. It is shown that the precipitation of ωiso phase retards or prevents the precipitation of nanometer size precipitates occurring between 450 and 500°C. This cannot be generalized to all the β-metastable titanium alloys since Ti-LCB does not exhibit the same heating rate dependence on DSC curves. [less ▲]

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See detailCharacterization of thin layers into concrete with Ground Penetrating Radar
Van der Wielen, Audrey ULg

Doctoral thesis (2014)

The Ground Penetrating Radar (GPR) is a non-destructive method used for roads and buildings inspection: it is well adapted to detect the different layers constituting the structures. The objective of this ... [more ▼]

The Ground Penetrating Radar (GPR) is a non-destructive method used for roads and buildings inspection: it is well adapted to detect the different layers constituting the structures. The objective of this thesis is to develop a method for a fast determination of the properties of a buried layer on the basis of surface GPR measurements. The observation of the GPR waves propagation in numerical simulations led to develop an analytical model, which could be applied to the experimental determination of laboratory layered structures. [less ▲]

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See detailCharacterization of thixoforming process of 100Cr6 steel
Solek, Krzysztof; Rassili, Ahmed ULg; Pierret, Jean-Christophe ULg et al

in Transactions of nonferrous metals society of China (2010), 20(special 3), 916-920

The main goal of this work is an analysis of the semi-industrial process of steel thixoforming. The process was carried out using industrial equipment. This equipment consists of a heating device ... [more ▼]

The main goal of this work is an analysis of the semi-industrial process of steel thixoforming. The process was carried out using industrial equipment. This equipment consists of a heating device, industrial robots and a hydraulic press. The globular microstructure ensuring thixotropic properties was obtained using the SIMA method. It is one of the simpler and cheaper methods which could be easy applied in case of steel alloys. In this work the hot forged rods, commercially produced from 100Cr6 steel, were used. The first part of the work concerned the determination of the proper temperature range, for thixoforming of 100Cr6 steel. Next, some heating tests were carried out in order to obtain as uniform temperature distribution as possible. Heating process was executed using inductive heating. Microstructure analysis of heated samples reveals globular particles surrounded by liquid phase. Next the thixoforming process was carried out using closed-die forming technique. Completely filled die cavity and good microstructure of the part show that applied process parameters were properly selected. [less ▲]

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See detailCharacterization of three new carboxylic ester hydrolases isolated by functional screening of a forest-soil metagenomic library
Biver, Sophie ULg; Vandenbol, Micheline ULg

in Journal of Industrial Microbiology & Biotechnology (2013), 40(2), 191-200

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See detailCharacterization of tillage effects on the spatial variation of soil properties using ground-penetrating radar and electromagnetic induction
Jonard, François; Mahmoudzadeh, Mohammad; Roisin, Christian et al

in Geoderma (2013), 207

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See detailCharacterization of Toxins within Crude Venoms by Combined Use of Fourier Transform Mass Spectrometry and Cloning
Quinton, Loïc ULg; Le Caër, Jean-Pierre; Phan, Gilles et al

in Analytical Chemistry (2005), 77(20), 6630-6639

The standard analytical procedure for screening the proteomic profile of a venom often relies on an appropriate combination of sample extraction, electrophoresis, reversed- phase high-performance liquid ... [more ▼]

The standard analytical procedure for screening the proteomic profile of a venom often relies on an appropriate combination of sample extraction, electrophoresis, reversed- phase high-performance liquid chromatography, mass spectrometry, and Edman degradation. We present in this study a new approach for venom screening based on Fourier transform mass spectrometry (FTMS) analysis directly on the crude venom. The venom chosen is a unique sample from Atractaspis irregularis, a species never studied at the molecular level previously. This snake belongs to the Atractaspidae family that is known to produce highly toxic venoms containing endothelin-like peptides called sarafotoxins (SRTXs). Nanoelectrospray- FTMS spectrum of the crude venom allowed the identification of 60 distinct compounds with molecular masses from 600 to 14 000 Da, which would have been impossible without the resolution of this kind of instrument. De novo sequencing within the entire venom confirmed the sequences of two new families of sarafotoxins, whose precursors had been cloned, and allowed the characterization of a third one. One particularly interesting point was that the propolypeptides appeared processed not in one unique compound, but rather in different length molecules ranging from 15 for the shorter to 30 amino acids for the longer. Moreover, our results clearly establish that in the case of A. irregularis only one copy of mature sarafotoxin emerges from each precursor, which is a totally different organization in comparison of other precursors of SRTXs. [less ▲]

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See detailCharacterization of tridimensional mixed cultures of mouse B16 melanoma cells and 3T3 fibroblasts
Coucke, Paul; Siwek, Brigitte; Munaut, Carine ULg et al

Conference (1990, September)

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See detailCharacterization of TRIP-assisted multiphase steel surface topography by atomic force microscopy
Ros-Yanez, Tanya; Houbaert, Yvan; Mertens, Anne ULg

in Materials Characterization (2001), 47

Transformation-induced plasticity (TRIP)-assisted multiphase steels have a complicated microstructure consisting of different phases, mainly ferrite, retained austenite, bainite and martensite. Atomic ... [more ▼]

Transformation-induced plasticity (TRIP)-assisted multiphase steels have a complicated microstructure consisting of different phases, mainly ferrite, retained austenite, bainite and martensite. Atomic force microscopy has been used for the phase identification and characterization of the phases in this kind of steel. A series of tests has been made on a C-Mn-Si and a C-Mn-Al TRIP-assisted steel after two different heat treatments: intercritical annealing followed by quench, and intercritical annealing followed by aging. After the aging process, the C-Mn-Al alloy was tempered in order to make metallographic observation easier, except the samples for mechanical testing, XRD or Mössbauer spectroscopy. It has been possible to identify the different phases and their topographic characteristics and to study their morphology using atomic force microscopy. The fine and complex microstructures of TRIP-assisted multiphase steels require improvements of the existing observation techniques, like electron backscattered diffraction and atomic force microscopy. Results of these techniques are presented. [less ▲]

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See detailCharacterization of tumor heterogeneity using bioimage informatics and 3D computational modeling
Stamatelos, Spyros; Carlier, Aurélie ULg; Kim, Eugene et al

Conference (2013, September 25)

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See detailCharacterization of Tunisian pomegranatenext term (Punica granatum L.) cultivars using amplified fragment length polymorphism analysis
Jbir, Rania; Hasnaoui, Nejib ULg; Mars, Messaoud et al

in Scientia Horticulturae (2008), 115(3), 231-237

The amplified fragment length polymorphism (AFLP) analysis of DNA was used to characterize 34 previous termpomegranatenext term cultivars. By using a combination of six primers, a total of 327 markers ... [more ▼]

The amplified fragment length polymorphism (AFLP) analysis of DNA was used to characterize 34 previous termpomegranatenext term cultivars. By using a combination of six primers, a total of 327 markers were scored with a mean of 57.5. The high percentage of polymorphic bands (ppb) of 94.7 and the resolving power (Rp) collective rate value of 129.14 were scored. Data proved that the tested primers were informative to discriminate among cultivars and to survey the genetic diversity in this fruit crop. It has been assumed that the local previous termpomegranatenext term germplasm is characterized by a typically continuous genetic diversity. The derived dendrogram proved that cultivars are clustered independently from their geographical origin and their denomination. In addition, AFLP permitted the generation of a nearly unlimited number of molecular markers that are reliable in differentiating the cultivars and/or the polyclonal varieties. [less ▲]

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See detailCharacterization of two Acacia gums and their fractions using a Langmuir film balance
Fauconnier, Marie-Laure ULg; Blecker, Christophe ULg; Groyne, J. et al

in Journal of Agricultural and Food Chemistry (2000), 48(7), 2709-2712

The mechanical properties of monolayers from two Acacia gums [Acacia senegal (L.) Willd. and Acacia seyal Del.] and their three fractions isolated by hydrophobic interaction chromatography were studied ... [more ▼]

The mechanical properties of monolayers from two Acacia gums [Acacia senegal (L.) Willd. and Acacia seyal Del.] and their three fractions isolated by hydrophobic interaction chromatography were studied with a Langmuir film balance to obtain a more complete understanding of their action mode. The analysis of compression isotherms revealed that A. senegal gums globally exhibit better interfacial properties than A. seyal ones. The behavior of the whole gums appeared to be strongly influenced by their arabinogalactan-protein complex. [less ▲]

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See detailCharacterization of two genes encoding the mitochondrial alternative oxidase in Chlamydomonas reinhardtii
Dinant, Monique; Baurain, Denis ULg; Coosemans, Nadine ULg et al

in Current Genetics (2001), 39(2), 101-108

Two cDNA clones (AOX1 and AOX2) and the corresponding genes encoding the alternative oxidases (AOXs) from Chlamydomonas reinhardtii were isolated and sequenced. The cDNAs, AOX1 and AOX2, contained open ... [more ▼]

Two cDNA clones (AOX1 and AOX2) and the corresponding genes encoding the alternative oxidases (AOXs) from Chlamydomonas reinhardtii were isolated and sequenced. The cDNAs, AOX1 and AOX2, contained open reading frames (ORFs) encoding putative proteins of 360 amino acids and 347 amino acids, respectively. For each of the ORFs, a potential mitochondrial-targeting sequence was found in the 5'-end regions. In comparison to AOX enzymes from plants and fungi, the predicted amino acid sequences of the ORFs showed their highest degree of identity with proteins from Aspergillus niger (38.1% and 37.2%) and Ajellomyces capsulatus (37% and 34.9%). Several residues supposed either to be Fe ligands or to be involved in the ubiquinol-binding site were fully conserved in both C. reinhardtii putative AOX proteins. In contrast, a cysteine residue conserved in the sequences of all higher plants and probably involved in the regulation of the enzyme activity was missing both from the AOX1 and AOX2 amino acid sequences and from protein sequences from various other microorganisms. The transcriptional expression of the AOX1 and AOX2 genes in wild-type cells and in mutant cells deficient in mitochondrial complex III activity was also investigated. [less ▲]

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See detailCharacterization of two structural aberrations in the horse by FISH with BAC clones.
Durkin, Keith ULg; Raudsepp, T; Chowdhary, B.P.

in Chromosome Research : An International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology (2006), 14

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