Browsing
     by title


0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

or enter first few letters:   
OK
Full Text
Peer Reviewed
See detailCharacterization of a single strong tissue-specific enhancer downstream from the three human genes encoding placental lactogen
Jacquemin, Patrick; Oury, Cécile ULg; Peers, Bernard ULg et al

in Molecular & Cellular Biology (1994), 14(1), 93-103

The human genes coding for growth hormone (hGH) and placental lactogen (choriosomatomammotropic hormone [hCS]) are clustered on chromosome 17 in the following order: 5' hGH-N hCS-L hCS-A hGH-V hCS-B 3 ... [more ▼]

The human genes coding for growth hormone (hGH) and placental lactogen (choriosomatomammotropic hormone [hCS]) are clustered on chromosome 17 in the following order: 5' hGH-N hCS-L hCS-A hGH-V hCS-B 3'. So far, a single placenta-specific enhancer has been identified in the locus, 2 kb downstream from the hCS-B gene, and shown to comprise one in vitro binding site for a nuclear protein. We here provide evidence that the hCS-B enhancer is more complex: (i) protection against DNase I digestion in the 3' flanking region of the hCS-B gene reveals four binding sites (DF-1, DF-2, DF-3, and DF-4) for nuclear proteins from either placental or HeLa cells, and (ii) placenta-specific enhancer activity can be fully exerted in transient expression experiments by a 126-bp fragment comprising the DF-3 and DF-4 protein-binding sites. By dissecting this region, we show that enhancer activity is mediated by a synergy between DF-3 and DF-4. Competitions with various oligonucleotides in footprinting and gel retardation experiments indicate that the same protein or set of proteins, different in HeLa and placenta cell nuclei, interacts with sites DF-2, DF-3, and DF-4. We also studied the regions of the hCS-L and hCS-A genes which are highly similar to the hCS-B enhancer. Although they each present the same four protein-binding sites, they exhibit only minor enhancer activity. [less ▲]

Detailed reference viewed: 22 (2 ULg)
See detailCharacterization of acetification enzymes from thermotolerant acetic acid bacteria, Acetobacter pasteurianus VM 136.
Mouanda, P.; Thonart, Philippe ULg; Tanazefti, H. et al

in Mededelingen Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen Universiteit Gent (1999), 64(5a),

Detailed reference viewed: 12 (0 ULg)
Full Text
Peer Reviewed
See detailCharacterization of Adulteration of "Espinheira Santa" ( Maytenus ilicifolia and Maytenus aquifolium, Celastraceae) Hydroalcoholic Extracts with Sorocea bomplandii ( Moraceae) by High-Performance Thin Layer Chromatography
Vilegas, Janete H.Y.; Lanças, Fernando M.; Wauters, Jean-Noël ULg et al

in Phytochemical Analysis (1998), 9

A HPTLC procedure for the characterization of adulteration of " espinheira santa ( Maytenus sp) with Sorocea bomplandii by monitoring their flavonoid content with the aid of diphenylboric reagent, is ... [more ▼]

A HPTLC procedure for the characterization of adulteration of " espinheira santa ( Maytenus sp) with Sorocea bomplandii by monitoring their flavonoid content with the aid of diphenylboric reagent, is presented. HPTLC data showed the predominance of higly glycosated quercetin and kaempferol derivatives. The derivated flavonoids were shown to be best markers for distinguishing between authentic and adulterated drug samples, with several advantages over triterpene analysis [less ▲]

Detailed reference viewed: 36 (2 ULg)
Full Text
See detailCHARACTERIZATION OF AGRICULTURAL SPRAY BY DIGITAL ANALYSIS OF SHADOWGRAPHY IMAGES
De Cock, Nicolas ULg; Massinon, Mathieu ULg; Lebeau, Frédéric ULg

Poster (2013, February 08)

Agricultural sprays are among the most common two-phase flows studied because their characteristics determine the efficiency of treatment as well as environmental contamination. At present, the relevance ... [more ▼]

Agricultural sprays are among the most common two-phase flows studied because their characteristics determine the efficiency of treatment as well as environmental contamination. At present, the relevance of different characterization techniques of the highly polydispersed sprays used in this application remains controversial. Digital analysis of shadowgraphy images presents an attractive option for the characterization of both velocity and size of droplets present in the spray. This study presents an overview of the contrast problems inherent in the use of backlighted images and propose appropriate solution to ensure the quality of measurements. Generally, background light presents heterogeneities associated with light sources and optical arrangement. These can be solved by substracting from each images a composite background. An other particular focus is given to one major drawback of volumetric lighting, the presence of out focus droplets. These droplets have to be removed during the image analysis process because the measurement of their diameters can't be done accuratly. The rejection of these out of focus objects is based on a quantitative parameter which was calibrated with a obliquely shot monodispersed spray. The final step of the image processing is determining velocity of the droplet by tracking a same droplet on two successive images. The tracking algorithm is based on the size of the droplet, its more probable displacement and its direction. [less ▲]

Detailed reference viewed: 39 (12 ULg)
Full Text
Peer Reviewed
See detailCharacterization of Alder Phytophthora Isolates from Wallonia and Development of SCAR Primers for their Specific Detection
De Merlier, D.; Chandelier, A.; Debruxelles, N. et al

in Journal of Phytopathology (2005), 153(2), 99-107

Isolates of alder Phytophthora were collected in the southern part of Belgium on riverbanks planted with Alnus glutinosa and A. incana. They were compared with strains isolated in other European countries ... [more ▼]

Isolates of alder Phytophthora were collected in the southern part of Belgium on riverbanks planted with Alnus glutinosa and A. incana. They were compared with strains isolated in other European countries in terms of maximum temperature for growth, oogonia shape, pathogenicity on Alnus seedlings and genetic traits. Using both molecular techniques [random amplified polymorphic DNA (RAPD) and random amplified microsatellite (RAMS)], two groups of isolates were identified, the first group being further divided into two subgroups, Ia and Ib, using RAPD. Most of the Walloon alder Phytophthora isolates as well as the standard type from UK (formally designated P. alni subsp. alni) fell into group Ia. One isolate was classified in group Ib with the German and Dutch variants (P. alni subsp. multiformis), while three isolates were placed with the Swedish variant (P. alni subsp. uniformis) in group II. In terms of morphological properties, isolates from groups Ia and Ib developed colonies with a felt-like appearance and usually produced numerous oogonia, varying from wavy to warty after 1 week (group Ia) or 2–3 weeks (Ib) in darkness. In contrast, colonies from group II isolates were generally irregular, and smooth oogonia were produced in low quantities after approximately 1 month in culture. A polymerase chain reaction (PCR) using sequence-characterized amplification region (SCAR) primers derived from a polymorphic amplification product generated with a RAPD primer was developed for the specific detection of alder Phytophthora. The specificity and sensitivity of this test are discussed here. [less ▲]

Detailed reference viewed: 50 (2 ULg)
See detailCharacterization of algae to produce biofuels
Vigeolas, Hélène ULg

Scientific conference (2012, August 27)

Detailed reference viewed: 26 (4 ULg)
Full Text
Peer Reviewed
See detailCharacterization of almond kernel oils of five almonds varieties cultivated in Eastern Morocco
Houmy, Nadia; Mansouri, F.; Benmoumen, A. et al

in Cahiers Options Méditerranéennes (2016)

This study focuses on characterization of almondkernel oils extrated mechanically from five sweet almond varieties (Marcona, Fournat, Ferragnes, Ferraduel and Beldi), cultivated in eastern Morocco. Oil ... [more ▼]

This study focuses on characterization of almondkernel oils extrated mechanically from five sweet almond varieties (Marcona, Fournat, Ferragnes, Ferraduel and Beldi), cultivated in eastern Morocco. Oil content, physicochemical parameters, triacylglycerol and fatty acid compositions were determined. [less ▲]

Detailed reference viewed: 21 (3 ULg)
Full Text
See detailCharacterization of almond kernel oils of five almonds varieties cultivated in Eastern Morocco
Houmy, Nadia ULg; Mansouri, Farid; Ben Moumen, Abdessamad et al

Poster (2015, May 03)

This study focuses on characterization of almond kernel oils extracted mechanically from five sweet almond varieties Marcona, Fournat, Ferragnes, Ferraduel and Beldi), cultivated in eastern Morocco. Oil ... [more ▼]

This study focuses on characterization of almond kernel oils extracted mechanically from five sweet almond varieties Marcona, Fournat, Ferragnes, Ferraduel and Beldi), cultivated in eastern Morocco. Oil content, physicochemical parameters, triacylglycerol and fatty acid compositions were determined. Analyzed oils showed low acidity values that range between 0.77 – 0.88 %, peroxide values range between 6.43 – 16.39 meq/kg and Iodine values range between 98.42 – 103.90%. The principal fatty acid of almond kernel oils is oleic acid (C18:1); oils of Ferragnes-Ferraduel and Beldi varieties show higher values of C18:1 respectively of 72.87 and 71.62 %, however Fournat almond kernel oil shows the lowest content of C18:1 (63.54%). HPLC analysis of triglycerides was carried out, and results show that analyzed almond kernel oils are characterized by the dominance of trioleylglycerol (OOO) that contents range between a minimum of 31.48 % for Fournat’s oil and 43.82% for Ferragnes-Ferraduel’s oil. The oxidative stability of almond kernel oils was determined by rancimat tests as the induction period (IP, h recorded by a 743 Rancimat apparatus Metrohm, Switzerland). Results show that stability, of almond kernel oils is clearly influenced by the almond variety; Oxidative stability of tested almond kernel oils ranged between an IP = 20.28 h for Marcona oil and an IP =27.55 h for Ferragnes-Ferraduel. [less ▲]

Detailed reference viewed: 41 (6 ULg)
Full Text
Peer Reviewed
See detailCharacterization of amylolysin, a novel lantibiotic from Bacillus amyloliquefaciens GA1
Arguelles Arias, Anthony ULg; Ongena, Marc ULg; Devreese, Bart et al

in PLoS ONE (2013), 8(12),

Background: Lantibiotics are heat-stable peptides characterized by the presence of thioether amino acid lanthionine and methyllanthionine. They are capable to inhibit the growth of Gram-positive bacteria ... [more ▼]

Background: Lantibiotics are heat-stable peptides characterized by the presence of thioether amino acid lanthionine and methyllanthionine. They are capable to inhibit the growth of Gram-positive bacteria, including Listeria monocytogenes, Staphylococcus aureus or Bacillus cereus, the causative agents of food-borne diseases or nosocomial infections. Lantibiotic biosynthetic machinery is encoded by gene cluster composed by a structural gene that codes for a pre-lantibiotic peptide and other genes involved in pre-lantibiotic modifications, regulation, export and immunity. Methodology/Findings: Bacillus amyloliquefaciens GA1 was found to produce an antimicrobial peptide, named amylolysin, active on an array of Gram-positive bacteria, including methicillin resistant S. aureus. Genome characterization led to the identification of a putative lantibiotic gene cluster that comprises a structural gene (amlA) and genes involved in modification (amlM), transport (amlT), regulation (amlKR) and immunity (amlFE). Disruption of amlA led to loss of biological activity, confirming thus that the identified gene cluster is related to amylolysin synthesis. MALDI-TOF and LC-MS analysis on purified amylolysin demonstrated that this latter corresponds to a novel lantibiotic not described to date. The ability of amylolysin to interact in vitro with the lipid II, the carrier of peptidoglycan monomers across the cytoplasmic membrane and the presence of a unique modification gene suggest that the identified peptide belongs to the group B lantibiotic. Amylolysin immunity seems to be driven by only two AmlF and AmlE proteins, which is uncommon within the Bacillus genus. Conclusion/Significance: Apart from mersacidin produced by Bacillus amyloliquefaciens strains Y2 and HIL Y-85,544728, reports on the synthesis of type B-lantibiotic in this species are scarce. This study reports on a genetic and structural characterization of another representative of the type B lantibiotic in B. amyloliquefaciens. Copyright: © 2013 Arguelles Arias et al. [less ▲]

Detailed reference viewed: 53 (21 ULg)
Full Text
Peer Reviewed
See detailCharacterization of an antibody panel for immunohistochemical analysis of canine muscle cells
Gofflot, Stéphanie ULg; Kischel, Philippe ULg; Thielen, Caroline ULg et al

in Veterinary Immunology and Immunopathology (2008), 125(3-4), 225-33

Immunohistochemistry is an indispensable tool in the assessment and characterization of lineage-specific differentiation of grafted cells in cell-based-therapy. This strategy is under investigation for ... [more ▼]

Immunohistochemistry is an indispensable tool in the assessment and characterization of lineage-specific differentiation of grafted cells in cell-based-therapy. This strategy is under investigation for the treatment of many muscle disorders and different animals such as dogs are used as models to study the tissue regeneration. The aim of the present study was to characterize an antibody panel for the analysis of canine muscle cells, useful in routinely processed formalin-fixed paraffin-embedded tissues. Overall, 12 antibodies (8 mouse monoclonal and 4 goat polyclonal), validated for use on human tissues tested for cross-reactivity on canine smooth muscle (bladder, intestine, and uterus), skeletal muscle and heart. Specific staining was achieved with eight antibodies, of which six were cytoplasmic markers (desmin, HDAC8, MHC, SMA, Troponin I and Troponin T) and two were cardiac nuclear markers (GATA-4 and Nkx-2.5). This antibody panel may be useful not only for the evaluation of cell-based therapies in muscle disorders, but also for the evaluation of canine soft tissue neoplasms in veterinary pathology. [less ▲]

Detailed reference viewed: 31 (10 ULg)
Full Text
Peer Reviewed
See detailCharacterization of an Enterococcus Hirae Penicillin-Binding Protein 3 with Low Penicillin Affinity
Piras, Graziella; El Kharroubi, Aboubaker; van Beeumen, Jozef et al

in Journal of Bacteriology (1990), 172(12), 6856-6862

Enterococcus hirae S185, a clinical isolate from swine intestine, exhibits a relatively high resistance to penicillin and contains two 77-kDa penicillin-binding proteins 3 of high (PBP 3s) and low (PBP 3r ... [more ▼]

Enterococcus hirae S185, a clinical isolate from swine intestine, exhibits a relatively high resistance to penicillin and contains two 77-kDa penicillin-binding proteins 3 of high (PBP 3s) and low (PBP 3r) affinity to penicillin, respectively. A laboratory mutant S185r has been obtained which overproduces PBP 3r and has a highly increased resistance to penicillin. Peptide fragments specifically produced by trypsin and SV8 protease digestions of PBP 3r were isolated, and the amino acid sequences of their amino terminal regions were determined. On the basis of these sequences, oligonucleotides were synthesized and used as primers to generate, by polymerization chain reaction, a 233-bp DNA fragment the sequence of which translated into a 73-amino-acid peptide segment of PBP 3r. These structural data led to the conclusion that the E. hirae PBP 3r and the methicillin-resistant staphylococcal PBP 2' are members of the same class of high-Mr PBPs. As shown by immunological tests, PBP 3r is not related to PBP 3s but, in contrast, is related to the 71-kDa PBP 5 of low penicillin affinity which is responsible for penicillin resistance in E. hirae ATCC 9790 and R40. [less ▲]

Detailed reference viewed: 18 (5 ULg)
See detailCharacterization of an in vivo model of VZV latency in the nervous system
Sadzot-Delvaux, Catherine ULg; Nikkels, Arjen ULg; Debrus, S. et al

Conference (1994)

Detailed reference viewed: 7 (0 ULg)
Full Text
Peer Reviewed
See detailCharacterization of an Insulating Material with regards to ECCS. Recommendations for the Fire Safety of Steel Structures
Bruls, Aloïs; Cajot, Louis-Guy; Franssen, Jean-Marc ULg

in Journal of Constructional Steel Research (1988), 9(2), 111-135

How to characterize an insulating product by an apparent constant thermal conductivity

Detailed reference viewed: 48 (5 ULg)
Full Text
See detailCharacterization of an internal type-II NADH dehydrogenase from Chlamydomonas reinhardtii mitochondria
Remacle, Claire ULg

in 15th International Conference on the Cell & Molecular Biology of Chlamydomonas (2012, June)

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailCharacterization of an original model of myocardial infarction provoked by coronary artery thrombosis induced by ferric chloride in pig
Dogné, Jean-Michel ULg; Rolin, Stéphanie; Petein, Michel et al

in Thrombosis Research (2005), 116(5), 431-442

Background: Great advances have been made in the prevention of thrombotic disorders by developments of new pharmacological and surgical treatments. Animal models of arterial thrombosis have largely ... [more ▼]

Background: Great advances have been made in the prevention of thrombotic disorders by developments of new pharmacological and surgical treatments. Animal models of arterial thrombosis have largely contributed to the discovery and to the validation of original treatments. The purpose of the present work was to develop and validate an original model of acute myocardial infarction provoked in pig by thrombosis of the left anterior descending (LAD) coronary artery induced by topical application of ferric chloride solution. Methods and results: Myocardial infarction, resulting from an occlusive and adherent mixed thrombus formed in the LAD coronary artery, was examined at macroscopic level using dual staining technique (Evans blue dye; triphenyltetrazolium chloride) and at microscopic level using conventional histological analyses and immunohistochemical detection of desmin. Biochemical markers (troponin T and ATP), platelet reactivity and standard hemodynamic parameters (such as stroke volume, ejection fraction, stroke work and cardiac output) have also been evaluated. From these analyses, it was demonstrated that each pig developed a transmural area of irreversible damage mainly located in the anteroseptal region of the left ventricle. The more progressive development of coronary artery occlusion, as compared to an abrupt Ligation, was accompanied by a correspondingly progressive impairment in hemodynamics. Conclusion: We conclude that this original porcine model of myocardial infarction is quite close to clinical pathophysiological conditions, such as thrombus formation occurring after atherosclerotic plaque rupture. This certainly constitutes a further argument in favour of this model to assess pharmaceutical or mechanical support of an acutely ischemic heart. (c) 2005 Elsevier Ltd. All rights reserved. [less ▲]

Detailed reference viewed: 82 (26 ULg)