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See detailComparative study of commercially available anti-alpha-synuclein antibodies.
Croisier, E.; MRes, D Elfant; Deprez, Manuel ULg et al

in Neuropathology & Applied Neurobiology (2006), 32(3), 351-6

Immunohistochemistry for alpha-synuclein has become the histological technique of choice for the diagnosis for Parkinson's disease, Dementia with Lewy bodies and Multiple System Atrophy (http://www.ICDNS ... [more ▼]

Immunohistochemistry for alpha-synuclein has become the histological technique of choice for the diagnosis for Parkinson's disease, Dementia with Lewy bodies and Multiple System Atrophy (http://www.ICDNS.org). Nevertheless, no standardised protocol has been proposed. We have reviewed 242 of the 270 studies published until June 2005 that mentioned immunohistochemistry for anti-alpha synuclein on human tissue and we found that only 75 (31%) used commercial antibodies. We also noted that protocols, particularly dilution and antigen unmasking, varied between studies, even when the same antibody was employed. In order to establish a standardised protocol for alpha-synuclein immunohistochemistry, which can be applied in diagnostic neuropathology we tested seven commercial monoclonal antibodies in brains of subjects with Parkinson's disease, dementia with Lewy bodies, multiple system atrophy, multiple sclerosis with incidental Lewy bodies and aged-matched normal brain and determined for each antibody the best suited protocol for antigen unmasking. We evaluated the intensity of immunolabelling in Lewy bodies, neuropil threads, dendrites, pre-synaptic terminals, granular cytoplasmic positivity, peri-axonal positivity, glial inclusions and non-specific immunolabelling. Although our results showed that all the antibodies detected alpha-synuclein inclusions, differences were noted between antibodies, particularly with regard to the detection of glial inclusions. From our study, the best antibodies of the seven tested appeared to be those directed against amino acids 116-131 and 15-123 and we suggest them to be used in routine diagnostic practice for alpha-synucleinopathies. [less ▲]

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See detailComparative study of copper surface treatment with self-assembled monolayers of aliphatic thiol, dithiol and dithiocarboxylic acid
Denayer, Jessica ULg; Delhalle, J.; Mekhalif, Z.

in Journal of Electroanalytical Chemistry (2009), 637(1-2), 43-49

The stability of alkanethiol self-assembled monolayers (SAMs) on metallic substrates, in particular copper, is an important aspect for their application. Some technologies, such as lithographic patterning ... [more ▼]

The stability of alkanethiol self-assembled monolayers (SAMs) on metallic substrates, in particular copper, is an important aspect for their application. Some technologies, such as lithographic patterning, can potentially benefit from the use of a transient easily removal protective coatings. Other applications like corrosion resistance, and thin-film lubrication request rather robust and stable films. The aim of this work consists in a comparative investigation of three organothiols (n-tetradecanethiol or RSH, 2-dodecylpropane-1,3-dithiol or R(SH)2 and n-tetradecanedithiocarboxylic or RS2H) on their ability to form SAMs on electrochemically reduced polycrystalline copper substrates and their respective stability. Characterizations of the SAMs are carried out using contact angle goniometry, XPS, PM-IRRAS and electrochemical studies (CV, LSV and cathodic desorption). R(SH)2 leads to monolayers with lower molecular organization than in the case of RSH and RS2H molecules. R(SH)2 monolayers are found the more stable when subjected to both anodic and cathodic desorption tests. © 2009 Elsevier B.V. All rights reserved. [less ▲]

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See detailComparative study of coronary flow reserve, coronary anatomy and results of radionuclide exercise tests in patients with coronary artery disease.
Legrand, Victor ULg; Mancini, G. B.; Bates, E. R. et al

in Journal of the American College of Cardiology (1986), 8(5), 1022-32

A comparative assessment of regional coronary flow reserve, quantitative percent diameter coronary stenosis and exercise-induced perfusion and wall motion abnormalities was performed in 39 patients with ... [more ▼]

A comparative assessment of regional coronary flow reserve, quantitative percent diameter coronary stenosis and exercise-induced perfusion and wall motion abnormalities was performed in 39 patients with coronary artery disease. Coronary flow reserve was determined by a digital angiographic technique utilizing contrast medium as the hyperemic agent. Percent diameter stenosis was calculated by an automated quantification program applied to orthogonal cineangiograms. Thallium-201 scintigraphy and radionuclide ventriculography were used to assess regional perfusion and wall motion abnormalities, respectively, at rest and during exercise. In Group A, 19 patients without transmural infarction or collateral vessels, coronary flow reserve was inversely related to percent diameter stenosis (r = -0.61, p less than 0.0001), and scintigraphic abnormalities occurred only in vascular distributions with a coronary flow reserve of less than 2.00. There was a strong relation among abnormal regional exercise results, stenoses greater than 50% and reactive hyperemia of less than 2.00. Patients with multivessel disease, however, often had normal exercise scintigrams in regions associated with greater than 50% stenosis and low coronary flow reserve when other regions had a lower coronary flow reserve or higher grade stenosis, or both. In Group B, 20 patients with angiographically visible collateral vessels, 12 of whom had prior myocardial infarction, coronary flow reserve correlated less well with percent diameter stenosis than in Group A (r = -0.47, p less than 0.004). As in Group A patients, there was a significant relation between abnormal exercise test results and stenoses greater than 50%. However, reactive hyperemia values were generally lower than in Group A, and positive exercise stress results were strongly correlated only with highly impaired flow reserves of 1.3 or less. In Group B patients, the coronary flow reserve of vessels with less than 50% stenosis was significantly lower than that of similar vessels in Group A patients (2.40 +/- 0.79 versus 1.56 +/- 0.43; p less than 0.0002). It is concluded that: there is a general relation between quantitative percent diameter stenosis and reactive hyperemia that is not of sufficient precision to allow accurate prediction of coronary flow reserve in individual cases; exercise scintigraphic abnormalities are usually associated with low coronary flow reserve, and the relation between these two functional tests is stronger than the relation between exercise test results and quantitative percent diameter stenosis.(ABSTRACT TRUNCATED AT 400 WORDS) [less ▲]

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See detailComparative study of decyl thiocyanate and decanethiol self-assembled monolayers on gold substrates
Dreesen, Laurent ULg; Volcke, C.; Sartenaer, Y. et al

in Surface Science (2006), 600

In a recent paper Ciszek et al. [J.W. Ciszek, M.P. Stewart, J.M. Tour, J. Am. Chem. Soc. 126 2004) 13172] showed that organic thiocyanates may be an interesting alternative to the use of thiols for ... [more ▼]

In a recent paper Ciszek et al. [J.W. Ciszek, M.P. Stewart, J.M. Tour, J. Am. Chem. Soc. 126 2004) 13172] showed that organic thiocyanates may be an interesting alternative to the use of thiols for thiolate assemblies. We use scanning tunnelling microscopy (STM), infrared reflection absorption and sum-frequency generation spectroscopies (IRRAS and SFG) in order to study the adsorption properties of decyl thiocyanates (DTCN) and compare them to the decanethiol (DT) ones. Firstly, IRRAS measurements show that DTCN molecules form self-assembled monolayers (SAMs) on gold via a thiolate link with the metallic substrate. Secondly, the DTCN SAM on gold is less ordered than the DT one as highlighted by SFG spectroscopy. Indeed, the intensities of the methyl vibration modes vanish while the methylene ones increase when DTCN molecules are adsorbed on the substrate instead of DT. We explain the differences in SAMs quality on the basis of STM measurements which reveal differences in molecular order and packing. [less ▲]

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See detailComparative study of decyl thiocyanate and decanethiol self-assembled monolayers on gold substrates
Dreesen, Laurent ULg; Volcke, Cédric; Sartenaer, Yannick et al

Poster (2005, September)

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See detailComparative study of dichlorodiphenyldichloroethylene in blood and semen of two young male populations: Lack of relationship to infertility, but evidence of high exposure of the mothers
Charlier, Corinne ULg; Foidart, Jean-Michel ULg

in Reproductive Toxicology (2005), 20(2, Jul-Aug), 215-220

The purpose of the study was to investigate the possible effect of an environmental organochlorine, dichlorodiphenyldichloroethylene (p,p'-DDE), on male fertility assessed by main sperm variables ... [more ▼]

The purpose of the study was to investigate the possible effect of an environmental organochlorine, dichlorodiphenyldichloroethylene (p,p'-DDE), on male fertility assessed by main sperm variables, conducted through a case-control study on young men attending the andrology laboratory in the context of infertility investigation in the couple. Blood and semen samples were obtained from 73 young men considered as fertile based on semen analysis (controls) and 82 classified as subfertile or unfertile (cases). Standard clinical analysis of semen was performed and identification and quantification of p,p'-DDE in serum and in seminal plasma were done by gas chromatography. No p,p'-DDE was detected in the seminal plasma of either group. Blood concentration of p,p'-DDE in both groups was very low and did not differ between cases and controls; however, blood samples were obtained from 23 mothers in the control group, and from 19 in the case group, and p,p'-DDE serum level was significantly higher in the mothers of subfertile men. These data suggest that male infertility could be associated with exposure of the mothers to p,p'-DDE with deleterious effects restricted to intra-uterine life and thus undetected in blood or seminal plasma of subfertile men. [less ▲]

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See detailComparative study of different lipases acting on milk fat globule membrane monolayers.
Danthine, Sabine ULg; Blecker, Christophe ULg; Deroanne, Claude

Poster (2003, September)

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See detailComparative study of durability test methods for pellets and briquettes
Temmerman, Michaël ULg; Rabier, Fabienne; Daugbjerg Jensen, Peter et al

in Biomass & Bioenergy (2006)

Different methods for the determination of the mechanical durability (DU) of pellets and briquettes were compared by international round robin tests including different laboratories. The DUs of five ... [more ▼]

Different methods for the determination of the mechanical durability (DU) of pellets and briquettes were compared by international round robin tests including different laboratories. The DUs of five briquette and 26 pellet types were determined. For briquettes, different rotation numbers of a prototype tumbler and a calculated DU index are compared. For pellets testing, the study compares two standard methods, a tumbling device according to ASAE S 269.4, the Lignotester according to O¨ NORM M 7135 and a second tumbling method with a prototype tumbler. For the tested methods, the repeatability, the reproducibility and the required minimum number of replications to achieve given accuracy levels were calculated. Additionally, this study evaluates the relation between DU and particle density. The results show for both pellets and briquettes, that the measured DU values and their variability are influenced by the applied method. Moreover, the variability of the results depend on the biofuel itself. For briquettes of DU above 90%, five replications lead to an accuracy of 2%, while 39 replications are needed to achieve an accuracy of 10%, when briquettes of DU below 90% are tested. For pellets, the tumbling device described by the ASAE standard allows to reach acceptable accuracy levels (1%) with a limited number of replications. Finally, for the tested pellets and briquettes no relation between DU and particle density was found. [less ▲]

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See detailComparative study of egg quality traits in two Belgian local breeds and two commercial lines of chickens
Moula, Nassim ULg; Antoine-Moussiaux, Nicolas ULg; Decuypere, Eddy et al

in Archiv Für Geglügelkunde = European Poultry Science = Revue de Science Avicole Européenne (2010), 74(3), 164-171

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See detailA comparative study of embryogenic and non-embryogenic cell cultures in Picea abies (L.) Karst.
Du Jardin, Patrick ULg; Chandelier, A.; Chauveau, F. et al

Book published by Elsevier (1996)

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See detailComparative study of four diagnostic methods of enzootic bovine leukemia.
Mammerickx, Marc; Burny, Arsène; Dekegel, D. et al

in Zentralblatt für Veterinarmedizin. Reihe B = Journal of Veterinary Medicine. Series B (1977), 24

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See detailComparative study of four diagnostic methods of enzootic bovine leukemia.
Mammerickx, Marc; Burny, Arsène; Dekegel, D. et al

in Burny, Arsène (Ed.) Bovine leucosis: various methods of molecular virology. (1977)

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See detailComparative study of four safflower oils (Carthamus tinctorius) varieties grown in eastern of Morocco
Ben Moumen, A.; Mansouri, F.; Zraibi, L. et al

Poster (2013, April)

Safflower (Carthamus tinctorius) is an oilseed crop, which, for many years, has been grown on a relatively small scale in parts of North Africa and Middle East (Purdy and al., 1959).

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See detailComparative study of four safflower oils (Carthamus tinctorius) varieties grown in eastern of Morocco
Ben Moumen, A.; Mansouri, F.; Zraibi, L. et al

in Book of Proceedings InsideFood Symposium (2013, April)

In this study, four safflower varieties, originating from Spain (Rancho), India (Sharda) and Morocco (Cartamar and Cartafri) cultivated at the experimental station of OUJDA (semi-arid region of Eastern ... [more ▼]

In this study, four safflower varieties, originating from Spain (Rancho), India (Sharda) and Morocco (Cartamar and Cartafri) cultivated at the experimental station of OUJDA (semi-arid region of Eastern Morocco) were evaluated for their oil yield and seed oil quality. The analysis of results revealed significant differences among varieties for all traits. Indeed, our result show that seeds of the four varieties have important oil content ranged between 35.38% (Rancho) and 28.84% (Cartamar). Characterization of these oils showed a low value of acidity index less than 0.7% for all the varieties but significant differences in their peroxide values ranged between 4.5 meq O2/Kg (Cartamar) and 31.21 mEqO2/Kg (Rancho) and high value of phenolic content between 143 ppm (Rancho) and 97 ppm (Sharda). Analysis of the fatty acids shows that linoleic acid is the main fatty acid ranged between 77.94% (Cartamar) and 79.98% (Sharda). Oils of those four varieties can be classified in the linoleic acid group. The analysis of triglycerides by HPLC shows 10 molecular species of TAG (LLL, LPL, LLO, PLO, LLS, POP, OOO, POO, SOO, PPL). The trilinoleate (LLL) is the main molecular species with more than 50%. Safflower seed oil cultivated in eastern Morocco presents good physicochemical proprieties (polyphenols and Omega 6 richness); therefore, safflower culture could be suggested for all areas of Morocco, including the areas of low pluviometry, while the potential for culture of other oilseeds is more limited. [less ▲]

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See detailComparative study of gold and silver based nanobiosensors
Lismont, Marjorie ULg; Dreesen, Laurent ULg

Poster (2011, May 25)

Due to their particular optical properties, resulting from the surface plasmon resonance (SPR) phenomenon, silver and gold nanoparticles (NPs) can be used as robust tools for optical biosensing [1 ... [more ▼]

Due to their particular optical properties, resulting from the surface plasmon resonance (SPR) phenomenon, silver and gold nanoparticles (NPs) can be used as robust tools for optical biosensing [1]. Optical SPR biosensors are able to measure complex formation in real time. Indeed, the SPR absorption spectrum band of the NPs is sensitive to the shape, size, inter-particle distance and composition of the NP as well as the dielectric properties of the surrounding medium [2]. Due to the sensitivity of SPR to the local dielectric environment, plasmonic NPs can act as transducers that convert small changes in the local refractive index and the inter-particle distance into spectral shifts and broadening in the absorption spectra bands [3]. In our study, the prototypical biocytin-avidin interaction was used to study gradual changes with time in the absorption spectra bands of biotinylated 10 nm silver and gold NPs as a function of added avidin. After avidin addition, a SPR red-shift and a broadening of the SPR bands were observed with both NPs. These optical changes evolved with time and reached their final values after around 45 min for each system. The maximum SPR red-shifts were equal to 25 nm and 15 nm for silver NPs and gold NPs, respectively. The detection limit, described as the lowest concentration for clear identification of wavelength shift due to biomolecular recognition, is determined to be 4 nM for both silver and gold NPs. The specificity of the biocytin-avidin biosensors was checked by replacing avidin by BSA. When BSA was added, we observed a SPR band shift which was smaller than the detection limit of 3 nm, attesting the biosensor selectivity. Our work demonstrates the superiority of Ag over Au NPs for the elaboration of biosensors based on SPR. [less ▲]

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See detailComparative Study of High Bio-Availability Glaphenine and Paracetamol in Cervical and Lumbar Arthrosis
Urbin Choffray, D.; Crielaard, Jean-Michel ULg; Albert, Adelin ULg et al

in Clinical Rheumatology (1987), 6(4), 518-25

Seventy-eight patients, 40 suffering from cervical arthrosis and 38 from lumbar arthrosis, received for a period of two weeks paracetamol or a new preparation of glaphenine, in a double blind study. The ... [more ▼]

Seventy-eight patients, 40 suffering from cervical arthrosis and 38 from lumbar arthrosis, received for a period of two weeks paracetamol or a new preparation of glaphenine, in a double blind study. The two drugs were found to have the same efficiency on the subjective parameters but high bioavailability glaphenine seems to have an effectiveness in the range of articulatory movements, which is not found with paracetamol. The side effects were quite equivalent. [less ▲]

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See detailA comparative study of isokinetic training using two brands of dynamometers
Croisier, Jean-Louis ULg; Herve, M. N.; Forthomme, Bénédicte ULg

in Abstract Book of the XIV International Congress on Sports Rehabilitation and Traumatology: «The Accelerated Rehabilitation of the Injured Athlete» (2005, April)

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See detailComparative study of mature and zymogen mite cysteine protease stability and pH unfolding.
Chevigne, A.; Dumez, Marie-Eve ULg; Dumoulin, Mireille ULg et al

in Biochimica et Biophysica Acta (2010), 1800(9), 937-945

BACKGROUND: Papain-like proteases (CA1) are synthesized as inactive precursors carrying an N-terminal propeptide, which is further removed under acidic conditions to generate active enzymes. METHODS: To ... [more ▼]

BACKGROUND: Papain-like proteases (CA1) are synthesized as inactive precursors carrying an N-terminal propeptide, which is further removed under acidic conditions to generate active enzymes. METHODS: To have a better insight into the mechanism of activation of this protease family, we compared the pH unfolding of the zymogen and the mature form of the mite cysteine protease Der p 1. RESULTS: We showed that the presence of the propeptide does not significantly influence the pH-induced unfolding of the catalytic domain but does affect its fluorescence properties by modifying the exposure of the tryptophan 192 to the solvent. In addition, we demonstrated that the propeptide displays weaker pH stability than the protease domain confirming that the unfolding of the propeptide is the key event in the activation process of the zymogen. GENERAL SIGNIFICANCE: Finally, we show, using thermal denaturation and enzymatic activity measurements, that whatever the pH value, the propeptide does not stabilize the structure of the catalytic domain but very interestingly, prevents its autolysis. [less ▲]

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