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See detailCellular thiamine status is coupled to function of mitochondrial 2-oxoglutarate dehydrogenase
Mkrtchyan, Garik; Graf, Anastasia; Bettendorff, Lucien ULg et al

in Neurochemistry International (2016), 101

Decreased thiamine and reduced activity of thiamine diphosphate (ThDP)-dependent 2-oxoglutarate dehydrogenase (OGDH) cause neurodegeneration. We hypothesized on concerted cell-specific regulation of the ... [more ▼]

Decreased thiamine and reduced activity of thiamine diphosphate (ThDP)-dependent 2-oxoglutarate dehydrogenase (OGDH) cause neurodegeneration. We hypothesized on concerted cell-specific regulation of the thiamine metabolism and ThDP-dependent reactions. We identified a smaller thiamine pool, a lower expression of the mitochondrial ThDP transporter, and a higher expression of OGDH in rat astrocytes versus neuroblastoma N2A. According to the data, the astrocytic OGDH may be up-regulated by an increase in intracellular ThDP, while the neuroblastomal OGDH functions at full ThDP saturation. Indeed, in rat astrocytes and brain cortex, OGDH inhibition by succinyl phosphonate (SP) enlarged the pool of thiamine compounds. Increased ThDP level in response to the OGDH inhibition presumably up-regulated the enzyme to compensate for a decrease in reducing power which occurred in SP-treated astrocytes. Under the same SP treatment of N2A cells, their thiamine pool and reducing power were unchanged, although SP action was evident from accumulation of glutamate. The presented data indicate that functional interplay between OGDH, other proteins of the tricarbocylic acid cycle and proteins of thiamine metabolism is an important determinant of physiology-specific networks and their homeostatic mechanisms. [less ▲]

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See detailCellular Uptake Of Antennapedia Penetratin Peptides Is A Two-Step Process In Which Phase Transfer Precedes A Tryptophan-Dependent Translocation
Dom, G.; Shaw-Jackson, C.; Matis, C. et al

in Nucleic Acids Research (2003), 31(2), 556-61

Several homeodomains and homeodomain-containing proteins enter live cells through a receptor- and energy-independent mechanism. Translocation through biological membranes is conferred by the third alpha ... [more ▼]

Several homeodomains and homeodomain-containing proteins enter live cells through a receptor- and energy-independent mechanism. Translocation through biological membranes is conferred by the third alpha-helix of the homeodomain, also known as Penetratin. Biophysical studies demonstrate that entry of Penetratin into cells requires its binding to surface lipids but that binding and translocation are differentially affected by modifications of some physico-chemical properties of the peptide, like helical amphipathicity or net charge. This suggests that the plasma membrane lipid composition affects the internalization of Penetratin and that internalization requires both lipid binding and other specific properties. Using a phase transfer assay, it is shown that negatively charged lipids promote the transfer of Penetratin from a hydrophilic into a hydrophobic environment, probably through charge neutralization. Accordingly, transfer into a hydrophobic milieu can also be obtained in the absence of negatively charged lipids, by the addition of DNA oligonucleotides. Strikingly, phase transfer by charge neutralization was also observed with a variant peptide of same charge and hydrophobicity in which the tryptophan at position 6 was replaced by a phenylalanine. However, Penetratin, but not its mutant version, is internalized by live cells. This underscores that charge neutralization and phase transfer represent only a first step in the internalization process and that further crossing of a biological membrane necessitates the critical tryptophan residue at position 6. [less ▲]

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See detailCellular uptake of liposomes monitored by confocal microscopy and flow cytometry
Ducat, Emilie ULg; Evrard, Brigitte ULg; Peulen, Olivier ULg et al

in Journal of Drug Delivery Science and Technology [=JDDST] (2011), 21(6), 469-477

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See detailCellular uptake of long-circulating pH-sensitive liposomes: evaluation of the liposome and its encapsulated material penetration in cancer cells
Ducat, Emilie ULg; Deprez, Julie ULg; Peulen, Olivier ULg et al

in Drug Discovery Today (2010, December), 15(23-24), 1079-1114

Print 3G, a peptidic antagonist of oncoprotein involved in breast cancer, could reduce the angiogenic development of breast tumors, leading to tumor dormancy. The necessity of intravenous administration ... [more ▼]

Print 3G, a peptidic antagonist of oncoprotein involved in breast cancer, could reduce the angiogenic development of breast tumors, leading to tumor dormancy. The necessity of intravenous administration of Print 3G led to the development of long-circulating liposomes as drug carriers. Pegylated liposomes, too large to be collected by fenestrated organs, accumulate passively in solid tumors thanks to the EPR effect. The strategy was to combine the protective properties of PEG with the transfection properties of pH-sensitive lipids which could promote the uptake of liposomes by cells and avoid lysosomal sequestration and degradation of entrapped materials such as peptides. In this study, we compare two formulations in terms of cellular uptake using confocal microscopy. The first one is composed of SPC:CHOL:mPEG-750-DSPE (47:47:6), used as "standard" liposomes, and the second one of DOPE:CHEMS:CHOL:mPEG750-DSPE (43:21:30:6), used as pH-sensitive liposomes. First, we evaluated the penetration of an encapsulated model molecule, calcein, in Hs578t human breast cancer epithelial cells. When calcein was encapsulated in standard liposomes, its penetration was effective only in few cells. On the contrary, a majority of cells were fluorescent when calcein-loaded pH-sensitive liposomes were applied for 3 hours on cells. Secondly, we studied the penetration of liposomes themselves in Hs578t cells using 25-[(nitrobenzoxadiazolyl)methylamino]nor-cholesterol (NBD-CHOL) as a fluorescent marker of the phospholipid membrane. The obtained results were comparable to those obtained with calcein: a higher penetration of liposome was observed for pH-sensitive liposomes. Finally, the cellular uptake of liposomes using both NBD-CHOL and rhodamine encapsulated in the inner cavity of vesicles was evaluated with Hs578t cells and compared with WI26 human diploid lung fibroblast cells. Thanks to this experiment, we could follow simultaneously the cell distribution of the encapsulated material and of the liposome itself. Confocal pictures obtained with pH-sensitive liposomes on both WI26 and Hs578t cells allow us to visualize the co-localized red and green colors of rhodamine and NBD-CHOL, with a higher concentrated area near the nucleus. In comparison with "standard" liposomes, we observed a higher penetration of the encapsulated material and of the liposome itself in breast cancer cells. Moreover, we visualized a colocalization near the nucleus of liposomes components. Concerning results obtained with fibroblastic cells, there was no difference in terms of cellular uptake between the two formulations. In perspective, we would like to compare these results, obtained with model molecules, with experiments performed with biotinylated Print 3G to assess its cellular distribution. Moreover, it would be interesting to correlate results obtained with confocal microscopy with a possible increase of the peptide efficacy against cancer cells when it is encapsulated in long-circulating pH-sensitive liposomes. [less ▲]

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See detailA cellulase from a psychrophilic microorganism: 3D structures of its native form and its complex with cellobiose
Violot, S.; Gouet, P.; Haser, Richard et al

Poster (2002)

Detailed reference viewed: 56 (0 ULg)
See detailCellulase hydrolysis Reactor : Effects of Agitation on cellulase activity.
Van Rollenghem, I.; Paquot, Michel ULg; Parajo, C. et al

Poster (1986, June)

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See detailCellulase involvement in the bacterial cellulose biosynthesis
Delsaute, Maud ULg; Berlemont, Renaud ULg; Bauvois, Cédric et al

Poster (2012)

Detailed reference viewed: 49 (11 ULg)
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See detailCellulase involvement in the cellulose biosynthesis of Pseudomonas stutzeri
Delsaute, Maud ULg; Berlemont, Renaud ULg; Paulus, Virginie et al

Poster (2011)

Detailed reference viewed: 34 (6 ULg)
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See detailCellulases catalysed cellulose polymerisation
Delsaute, Maud ULg; Berlemont, Renaud ULg; Renson, Thomas ULg et al

Poster (2010)

Detailed reference viewed: 40 (26 ULg)
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See detailCellule "Base De Données"
Govaerts, P.; Corten, P.; Costermans, D. et al

Report (2001)

Detailed reference viewed: 33 (5 ULg)
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See detailLa cellule beta dans le diabète de type II: coupable ou victime?
SCHEEN, André ULg; Paquot, Nicolas ULg; Lefebvre, P. J.

in Journées Annuelles de Diabetologie de l'Hôtel-Dieu (1991)

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See detailUne cellule d'encadrement pour les producteurs transformateurs
Helleputte, Murielle ULg; De Laubier, Juliette ULg; Di Tanna, Sybille ULg et al

in Wallonie Elevages (2012), 4

La Cellule Qualité Produits Fermiers possède un guichet unique auquel le producteur-transformateur peut s'adresser pour toute question relative à son projet de diversification. En fonction de la demande ... [more ▼]

La Cellule Qualité Produits Fermiers possède un guichet unique auquel le producteur-transformateur peut s'adresser pour toute question relative à son projet de diversification. En fonction de la demande, il est dirigé vers le ou les pôles compétents. [less ▲]

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See detailLa Cellule qualité des produits fermiers
Helleputte, Murielle ULg; Sindic, Marianne ULg

in Les filières bovines dans la tourmente : produire plus et mieux avec moins. (2009)

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See detailLa Cellule Qualité Produits Fermiers partenaire de diversification. Bilan microbiologique des produits laitiers artisanaux
Sanchez-Alcaraz, Maria-Thérésa ULg; Helleputte, Murielle ULg; Godrie, Thérèse ULg et al

in 16ème Carrefour des Productions animales : la filière laitière bovine est-elle durable? (2011, March)

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See detailCellules Bien-être. Repères : EvalCBE.1
Vandoorne, Chantal ULg

Diverse speeche and writing (2012)

Detailed reference viewed: 12 (0 ULg)