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See detailComparative evaluation of five hygroscopic condenser humidifiers during short-term postoperative mechanical ventilation
Sottiaux, Thierry; Meurice, F.; Mignolet, Ghislaine ULg et al

in Acta Anaesthesiologica Italica & Anaesthesia and Intensive Care in Italy (1997), 48(1-2), 59-68

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See detailComparative Evaluation of Four Detectors in the High-Performance Liquid Chromatographic Analysis of Chiral Nonaromatic Alcohols
Toussaint, B.; Duchateau, A. L.; van der Wal, S. et al

in Journal of Chromatographic Science (2000), 38(10), 450-7

A comparative evaluation of ultraviolet, polarimetric, refractive index, and evaporative light-scattering detection coupled with high-performance liquid chromatography has been developed for the ... [more ▼]

A comparative evaluation of ultraviolet, polarimetric, refractive index, and evaporative light-scattering detection coupled with high-performance liquid chromatography has been developed for the separation and quantitation of the enantiomers of chiral nonaromatic alcohols, some of which are intermediates in the synthesis of chiral drugs. (R,S)-3-tert-butylamino-1,2-propanediol; (R,S)-glycidol; and (R,S)-1-(4-morpholino)-2-octanol are selected as model compounds in order to compare the detection sensitivity and the linearity of the response with the four detectors. Separation of the enantiomers is performed using chiral stationary phases in normal-phase liquid chromatography. A one-day validation is achieved for (S)-3-tert-butylamino-1,2-propanediol with each detector, and limits of quantitation are determined for the three compounds. Advantages and limitations of the four detectors are discussed. [less ▲]

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See detailComparative evaluation of four detectors in the HPLC analysis of chiral non-aromatic alcohols
Toussaint, B.; Duchateau, A. L. L.; Van der Wal, S. et al

Conference (2000)

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See detailComparative evaluation of Fungitest, Neosensitabs and broth microdilution method for yeasts susceptibility testing
Hayette, Marie-Pierre ULg; Bolland, Pascal; Seidel, Laurence ULg et al

Poster (1999, May)

The need of a simple and reliable method for routine yeasts susceptibility testing led us to evaluate two commercially available methods. We investigated the in vitro susceptibility of 67 clinical ... [more ▼]

The need of a simple and reliable method for routine yeasts susceptibility testing led us to evaluate two commercially available methods. We investigated the in vitro susceptibility of 67 clinical isolates (26 C. albicans, 32 C. glabrata, 4 C. krusei, 2 C. tropicalis, 2 C. kefyr and 1 S. cerevisiae) to 6 drugs flucytosine (FC), amphotericin B (A), fluconazole (FZ), itraconazole (IT), kétoconazole (K), miconazole (M), comparing two methods Fungitest (Sanofi Pasteur) and Neosensitabs (Rosco). A broth microdilution adaptation from the NCCLS-M27A procedure was used as reference method. Fungitests consist of individually packed 16 wells microplates containing 6 drugs at two critical concentrations in buffered medium. Reading was performed after 24 and 48h incubation. Neosensitabs is an agar diffusion method on Shadomy agar using antifungals tablets. Reading was performed after 24h. For all strains Neosensitabs was in concordance with NCCLS M27-A for FC (94%), A (98%), FZ (55%) I (53%) with p<0.05. Fungitest correlated with NCCLS method for all antifungals after 24 and 48h incubation time (p<0.05) with respectively 95/95% for FC, 100/100% for A, 80/76% for FZ, 81/55% for I and 93/75% for K, and 88/81 for M. Candida glabrata gave the poorest result with Neosensitabs with 28% concordance for FZand 39% for I; therefore the method can't be recommended for this species. Fungitest concordance observed was 55% for I after 48h. Our results suggest that Fungitest is appropriate for routine yeast susceptibility testing. However itraconazole testing has to be improved with this method. [less ▲]

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See detailComparative evaluation of impulse oscillometry and a monofrequency forced oscillation technique in clinically healthy calves undergoing bronchochallenges
Reinhold, Petra; MacLeod, D.; Lekeux, Pierre ULg

in Research in Veterinary Science (1996), 61

Multifrequency impulse oscillometry (IOS) was compared with a monofrequency forced oscillation technique (MFO) in calves undergoing experimentally induced bronchoconstriction and subsequent ... [more ▼]

Multifrequency impulse oscillometry (IOS) was compared with a monofrequency forced oscillation technique (MFO) in calves undergoing experimentally induced bronchoconstriction and subsequent bronchodilatation. The dynamic lung compliance (Cdyn) was also measured by conventional methods. For each test, the baseline mean and the responses to saline, a bronchoconstrictive agent (carbachol) and a bronchodilator (fenoterolhydrobromide) were calculated. Using the IOS, the information was markedly frequency-dependent. The resistance (R) and the magnitude of respiratory impedance (Z) were only sensitive at 5 Hz, leading to negative frequency dependence of these parameters as an indicator of peripheral airway obstruction. A high sensitivity for reactance (X) and phase angle phi values was observed between 5 and 20 Hz. For MFO (10 Hz), the parameters Ros (which includes resistive and capacitive components of the respiratory system), phase shift (psi), and the oscillatory derived compliance of the respiratory system (Crs) were of the greatest clinical potential. Crs showed a significant coefficient of linear correlation (r = 0.88, P < 0.001) with Cdyn. At the 10 Hz test frequency similar results were observed with MFO and IOS, suggesting that for healthy calves the measurement effect of an impulse is not significant. With respect to peripheral airway calibre, a test frequency less than 10 Hz appeared to be most sensitive and least variable. [less ▲]

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See detailComparative evaluation of local poultry breeds status in Algeria, Vietnam and the Democratic Republic of Congo
Moula, Nassim ULg; Farnir, Frédéric ULg; Abdellah, Salhi et al

in The Role of Poultry in Improving Human Welfare (2012, September)

Local chicken breeds contribute significantly to the world production of meat and eggs. Indigenous breeds represent 80% of the world poultry population. However, the majority of these breeds has not been ... [more ▼]

Local chicken breeds contribute significantly to the world production of meat and eggs. Indigenous breeds represent 80% of the world poultry population. However, the majority of these breeds has not been recorded and studied. About 40% of poultry breeds have an unknown risk status. Hence, considerable efforts are necessary to evaluate them. Obviously, managing animal genetic resources requires the identification of the concerned phenotypes, population sizes, their geographical distribution, and their genetic diversity within and between breeds, using molecular biology methods. Nevertheless, the thorough understanding of the breeding contexts within which they are found is an oft-neglected prerequisite to set up sustainable management strategy of these resources. In the framework of our studies, the characterization of local poultry populations and their breeding systems have been carried out in Northeastern Algeria, Northern Vietnam and Southwestern Congo (DRC). A large phenotypic diversity has been revealed in each region of study, contributing different insights into the concept of local breed. The breeding systems have in common multiple objectives (nutritional, financial, and socio-cultural). Women and children played an important role in family poultry farming. The latter elements are, however, subject to variations between the regions of study and within them. Thus, solutions aiming at the promotion of biodiversity in poultry need to be tailored in accordance with the uniqueness of each breeding context. [less ▲]

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See detailComparative evaluation of three heat and moisture exchangers during short-term postoperative mechanical ventilation
Sottiaux, Thierry; Mignolet, Ghislaine ULg; Damas, Pierre ULg et al

in CHEST (1993), 104(1), 220-224

This study compared performance of three heat and moisture exchangers (HME) during short-term postoperative mechanical ventilation. Temperature and absolute humidity (AH) were measured at various points ... [more ▼]

This study compared performance of three heat and moisture exchangers (HME) during short-term postoperative mechanical ventilation. Temperature and absolute humidity (AH) were measured at various points of the ventilatory circuit. There was no statistical difference between the groups, regarding ambient and body To, body weight, fraction of inspired oxygen, tidal volume, and respiratory rate. Only the hygroscopic HME (groups 2 and 3) provide adequate conditioning with regard to AH and To of the inspiratory gases. The performance of hydrophobic HME (group 1) was inferior and appears to be unsatisfactory. Indirect evaluation (variations of inspiratory gases and tracheal temperatures, AH of the expired gases) confirmed the superiority of the hygroscopic HME. These data suggest that humidification of inspiratory gases with a hygroscopic HME is a defensible practice during short-term postoperative mechanical ventilation. Performance of hydrophobic HME may be weak and can expose the patient to an unacceptable risk of endotracheal tube occlusion. [less ▲]

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See detailComparative Fe and Zn K-edge X-ray absorption spectroscopic study of the ferroxidase centres of human H-chain ferritin and bacterioferritin from Desulfovibrio desulfuricans.
Toussaint, Louise ULg

in Journal of Biological Inorganic Chemistry : A Publication of the Society of Biological Inorganic Chemistry (2009), 14(1), 35-49

Iron uptake by the ubiquitous iron-storage protein ferritin involves the oxidation of two Fe(II) ions located at the highly conserved dinuclear ‘‘ferroxidase centre’’ in individual subunits. We have ... [more ▼]

Iron uptake by the ubiquitous iron-storage protein ferritin involves the oxidation of two Fe(II) ions located at the highly conserved dinuclear ‘‘ferroxidase centre’’ in individual subunits. We have measured X-ray absorption spectra of four mutants (K86Q, K86Q/E27D, K86Q/E107D, and K86Q/E27D/E107D, involving variations of Glu to Asp on either or both sides of the dinuclear ferroxidase site) of recombinant human H-chain ferritin (rHuHF) in their complexes with reactive Fe(II) and redoxinactive Zn(II). The results for Fe–rHuHf are compared with those for recombinant Desulfovibrio desulfuricans bacterioferritin (DdBfr) in three states: oxidised, reduced, and oxidised/Chelex -treated. The X-ray absorption nearedge region of the spectrum allows the oxidation state of the iron ions to be assessed. Extended X-ray absorption fine structure simulations have yielded accurate geometric information that represents an important refinement of the crystal structure of DdBfr; most metal–ligand bonds are shortened and there is a decrease in ionic radius going from the Fe(II) to the Fe(III) state. The Chelex -treated sample is found to be partly mineralised, giving an indication of the state of iron in the cycled-oxidised (reduced, then oxidised) form of DdBfr, where the crystal structure shows the dinuclear site to be only half occupied. In the case of rHuHF the complexes with Zn(II) reveal a surprising similarity between the variants, indicating that the rHuHf dinuclear site is rigid. In spite of this, the rHuHf complexes with Fe(II) show a variation in reactivity that is reflected in the iron oxidation states and coordination geometries. [less ▲]

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See detailComparative fluxes of HCO3- and Si from glaciated and non-glaciated terrain during the last deglaciation.
Jones, I. W.; Munhoven, Guy ULg; Tranter, M.

in Tranter, M.; Armstrong, R.; Brun, E. (Eds.) et al Interactions Between the Cryosphere, Climate and Greenhouse Gases (1999)

There is current interest in the riverine fluxes of bicarbonate and Si at the last glacial maximum (LGM), since modelling suggests that these were higher than today (Gibbs and Kump, 1994; Froelich et al ... [more ▼]

There is current interest in the riverine fluxes of bicarbonate and Si at the last glacial maximum (LGM), since modelling suggests that these were higher than today (Gibbs and Kump, 1994; Froelich et al., 1992). If this is the case, removal of atmospheric CO2 by silicate weathering is also likely to have been greater at the LGM (Munhoven and Francois, 1996), so contributing to the lower atmospheric CO2 recorded by ice cores (Barnola et al., 1987). To date, the magnitude of glacial chemical erosion on bicarbonate and Si fluxes at the LGM is poorly quantified, and the locus of the inferred doubled terrestrial Si flux is unknown. This paper aims to provide first estimates of the relative fluxes of bicarbonate and Si fluxes from ice-free and glaciated terrain for five time steps between the LGM (21ka), by extending the modelling approach of Gibbs and Kump (1994) and incorporating new data on glacial solute fluxes (Tranter et al., submitted). [less ▲]

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See detailComparative genome organization of vertebrates. The First International Workshop on Comparative Genome Organization.
Andersson, L.; Archibald, A.; Ashburner, M. et al

in Mammalian Genome : Official Journal of the International Mammalian Genome Society (1996), 7(10), 717-34

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See detailA comparative Genome-Wide Association Interaction study using BOOST and MB-MDR algorithms on Ankylosing Spondylitis
Bessonov, Kyrylo ULg

Poster (2013, April 29)

Genome-Wide Association (GWA) studies have gained popularity after the completion of the Human Genome Project and advancement of high-throughput technologies. These studies aim to scan thousands of ... [more ▼]

Genome-Wide Association (GWA) studies have gained popularity after the completion of the Human Genome Project and advancement of high-throughput technologies. These studies aim to scan thousands of genomic variations (e.g., SNPs) for their association to phenotypic variables (i.e. traits), such as disease related phenotypes, with the hope of extracting biologically and clinically relevant information. Understanding of genetic, environmental as well as other components of the disease brings the key insights into disease pathology and approaches us closer to the ultimate goal - personalized medicine. In this work we rely on a minimal GWAI protocol for genome-wide epistasis detection using SNPs, as developed in our lab [6][9]. Using the advanced non-parametric Model-Based Multifactor Dimensionality Reduction (MB-MDR) method [1] and BOolean Operation-based Screening and Testing (BOOST) algorithms [4][*] for detection of statistically significant epistatic SNP-SNP interactions, we investigate the effect of exhaustive (BOOST) and non-exhaustive (MB-MDR) marker processing strategies, LD effects, as well as different adjustment schemes for lower-order effects (i.e. epistasis). Our approach was tested on Ankylosing Spondylitis (AS) data as provided by the WTCCC2 consortium [1]. AS is a long-term / chronic disease characterized by inflammation of the joints between the spinal bones. Non-steroidal anti-inflammatory drugs calming down the immune system inflammatory responses are used as a treatment but there is no permanent cure for AS. The disease has also a strong environmental component and affects 3.5 - 13 per 1,000 people in USA [5] [less ▲]

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See detailComparative genomics to investigate the evolutionary development of the genus Bifidobacterium
Lugli, Gabriele; Milani, Christian; Turroni, Francesca et al

in Applied and Environmental Microbiology (2014)

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See detailComparative haemodynamic effects of intravenous flecainide in patients with and without heart failure and with and without beta-blocker therapy.
Legrand, Victor ULg; Materne, P.; Vandormael, M. et al

in European heart journal (1985), 6(8), 664-71

The haemodynamic effects of flecainide were compared in three different subsets of patients with documented coronary disease. Ten patients (A) had no heart failure, 5 patients were on beta blockers (B ... [more ▼]

The haemodynamic effects of flecainide were compared in three different subsets of patients with documented coronary disease. Ten patients (A) had no heart failure, 5 patients were on beta blockers (B) and 5 patients had overt heart failure (C). Flecainide was associated with negative inotropic effects that were relatively more pronounced in patients with left ventricular dysfunction: pulmonary wedge pressure increased by 27% in A, by 31% in B and by 42% in C; left ventricular stroke volume and stroke work decreased respectively by 10 and 12% in A, 21 and 19% in B, 26 and 28% in C. Ejection fraction decreased by 9% in A, 13% in B and 20% in C, in relation with an increase in end systolic volume (+9% in A, +10% in B and +5% in C). Absolute changes, however, were not significantly different from one group to another except for the increase of systemic vascular resistance which was more pronounced in C as compared with the other groups. The myocardial depression was also confirmed by the fall in dP/dt that was maximal at the end of injection; dP/dt remained depressed 15 min later despite some improvement. Flecainide thus exerts negative inotropic effects that are maximal at the end of infusion and may be of importance in patients with established left ventricular dysfunction. [less ▲]

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See detailComparative imaging of the podotrochlear apparatus in the horse
Schneider, Nicole ULg

Master of advanced studies dissertation (2000)

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See detailComparative immunohistochemical study of herpes-simplex and varicella-zoster infections
Nikkels, Arjen ULg; Debrus, S.; Sadzot-Delvaux, Catherine ULg et al

in Virchows Archiv. A : Pathological Anatomy and Histopathology (1993), 422(2), 121-126

Herpes simplex (HSV) and varicella-zoster (VZV) skin infections share so many histological similarities that distinguishing between them may prove to be impossible. We developed and characterized a new ... [more ▼]

Herpes simplex (HSV) and varicella-zoster (VZV) skin infections share so many histological similarities that distinguishing between them may prove to be impossible. We developed and characterized a new monoclonal antibody, VL8, IgG kappa isotype, directed to the VZV envelope glycoprotein gpI. Immunohistochemistry with VL8 appeared highly sensitive and specific on formalin-fixed paraffin-embedded biopsies and a clear-cut distinction between HSV and VZV infections was possible. The pattern of VL8 immunolabelling in VZV infections was strikingly different from that found in HSV infections studied with polyclonal antibodies to HSV I and II. Double immunolabelling revealed the VL8 positivity of sebaceous cells, endothelial cells, Mac 387-and CD68-positive monocyte-macrophages, and factor XIIIa-positive perivascular, perineural and interstitial dendrocytes. Intracytoplasmic VL8 labelling of endothelial cells and perivascular dendrocytes was found at the site of leukocytoclastic vasculitis. [less ▲]

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See detailComparative In Vitro Activity of Amphotericine B, Itraconazole, Voriconazole and Posaconazole against Aspergillus fumigatus
Hayette, Marie-Pierre ULg; Amadore, Agatha; Seidel, Laurence ULg et al

Poster (2001, December)

Background. New azoles have been successfully used as treatment of invasive aspergillosis. The purpose of this study was to compare the in vitro activity of posaconazole (Posa) with that of amphotericin B ... [more ▼]

Background. New azoles have been successfully used as treatment of invasive aspergillosis. The purpose of this study was to compare the in vitro activity of posaconazole (Posa) with that of amphotericin B (AmB), itraconazole (Itra) and voriconazole (Vor) against A. fumigatus isolates according to NCCLS method (M38-P), and to compare visual and spectrophotometric readings for MIC determination. Methods. A total of 106 A. fumigatus isolates were selected as follows: 88 clinical isolates from colonized patients, 18 from patients with invasive aspergillosis and 7 environmental isolates. Their in vitro susceptibility was evaluated by the NCCLS microdilution method (M38-P) in RPMI 1640 medium. Determination of results was made by visual and spectrophotometric readings (630 nm) after 48 hours incubation at 35 degreesC. Three A. fumigatus reference strains (IHEM 5734, 6149 and 13935) were included as control. Results. 1. Geometric mean MICs/MIC90 (microg/ml) obtained by visual reading were respectively 0.66/1 (AmB), 0.37/0.5 (Itra), 0.27/0.5 (Vor) and 0.02/0.03 (Posa). 2. MIC values were comparable by spectrophotometric and by visual readings for all antifungal agents tested (p>.05) and did not depend on the isolates origin (p>.05). 3. Posaconazole had the lowest MICs (p< 0.001). 4. The itraconazole-resistant reference strain did not give cross resistance with voriconazole and posaconazole. CONCLUSIONS: Among azoles, posaconazole had a better in vitro activity against A. fumigatus than did voriconazole or itraconazole. Spectrophotometric reading could replace the less standardized visual reading for NCCLS microdilution method and MIC values obtained were comparable among all A. fumigatus isolates. [less ▲]

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